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Protein_selection.md

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Protein selection

Difference between immunological fluorescence and fluorescence protein fusions.

Immunolabelling Fluorescent protein
probe palette larger, including organic dyes smaller
number of photons higher when using organic dye lower
Required condition harsh light

Kaede

rsEGFP

rsEGFP analysis

mEos

This protein does not suffer from oligomerization, small photon budgets or poor photostability.