diff --git a/h5pyd/_hl/files.py b/h5pyd/_hl/files.py
index 63b00c1e..a3a642ca 100644
--- a/h5pyd/_hl/files.py
+++ b/h5pyd/_hl/files.py
@@ -190,6 +190,10 @@ def __init__(self, domain, mode=None, endpoint=None, username=None, password=Non
                 elif "hs_endpoint" in cfg:
                     endpoint = cfg["hs_endpoint"]
 
+            # remove the trailing slash on endpoint if it exists
+            if endpoint.endswith('/'):
+                endpoint = endpoint.strip('/')
+                
             if username is None:
                 if "H5SERV_USERNAME" in os.environ:
                     username = os.environ["H5SERV_USERNAME"]