Host factor data were obtained from the Host_Factors_Summary.xlsx spreadsheet.
These host factors were the targets in the network flow analysis.
The sheet Comparison of RNAi Screens, And was used to create initial gene list files.
The first five columns are screen hits compiled from other researchers.
Mehle lab screens for influenza host factors are provided in the Our Hits sheet.
A sorted, concatenated list of the above screening results was then mapped to ENSP, filtered to only include the ENSP identifiers present in the STRINGdb network (experimental edges only), and named mehle_targets_ensp.txt.
That file was used as the --targets-file in flow.py.
mehle_validation_sources.txt is a short list of genes whose pathway activities are known.
It was used to verify that the flow method produces networks consistent with the well-studied pathways those genes are members of.
mehle_validation_sources_ensp.txt is the above file after mapping from HGNC to ENSP identifiers.