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Hi,
Using SURVIVOR v1.0.7 I've noticed that the size of translocations given in the VCF file generated by SURVIVOR simSV is not calculated correctly. For example, here is my .params file entry for TRA:
and here's the BED and VCF file entries generated for two TRA events:
BED:
Chr1 1366162 Chr3 1731342 TRA
Chr1 1376151 Chr3 1741331 TRA
Chr2 1896233 Chr3 1742907 TRA
Chr2 1906139 Chr3 1752813 TRA
VCF:
Chr1 1366162 TRA12SURVIVOR N <TRA> . LowQual PRECISE;SVTYPE=TRA;SVMETHOD=SURVIVOR_sim;CHR2=Chr3;END=1731342;SVLEN=365180 GT:GL:GQ:FT:RC:DR:DV:RR:RV 1/1
Chr1 1376151 TRA12SURVIVOR N <TRA> . LowQual PRECISE;SVTYPE=TRA;SVMETHOD=SURVIVOR_sim;CHR2=Chr3;END=1741331;SVLEN=365180 GT:GL:GQ:FT:RC:DR:DV:RR:RV 1/1
Chr2 1896233 TRA13SURVIVOR N <TRA> . LowQual PRECISE;SVTYPE=TRA;SVMETHOD=SURVIVOR_sim;CHR2=Chr3;END=1742907;SVLEN=-153326 GT:GL:GQ:FT:RC:DR:DV:RR:RV 1/1
Chr2 1906139 TRA13SURVIVOR N <TRA> . LowQual PRECISE;SVTYPE=TRA;SVMETHOD=SURVIVOR_sim;CHR2=Chr3;END=1752813;SVLEN=-153326 GT:GL:GQ:FT:RC:DR:DV:RR:RV 1/1
The TRA lengths should be 9989 for the first TRA and 9906 for the second TRA. What's happening is that the SVLEN is being calculated by subtracting the start position on 'CHR2' (the receiving chromosome) from the VCF 'POS' coordinate.
For example, in the first TRA between Chr1 and Chr3, it's subtracting 1366162 from 1731342 and calculating it as an SVLEN of 365180 and this is also why there's a negative value for the second TRA between Chr2 and Chr3 (1742907 - 1896233 = -153326).
Cheers,
Graham
The text was updated successfully, but these errors were encountered:
Hi,
Using SURVIVOR v1.0.7 I've noticed that the size of translocations given in the VCF file generated by
SURVIVOR simSV
is not calculated correctly. For example, here is my .params file entry for TRA:and here's the BED and VCF file entries generated for two TRA events:
BED:
VCF:
The TRA lengths should be 9989 for the first TRA and 9906 for the second TRA. What's happening is that the SVLEN is being calculated by subtracting the start position on 'CHR2' (the receiving chromosome) from the VCF 'POS' coordinate.
For example, in the first TRA between Chr1 and Chr3, it's subtracting 1366162 from 1731342 and calculating it as an SVLEN of 365180 and this is also why there's a negative value for the second TRA between Chr2 and Chr3 (1742907 - 1896233 = -153326).
Cheers,
Graham
The text was updated successfully, but these errors were encountered: