Tools to assist with designing multiple RT-PCR primers for full-length isoform amplification.
Package is currently alpha-quality and requires a bit manual setup to run.
This package builds on primer3 and Primer3-py.
Untergasser, Andreas, et al. "Primer3—new capabilities and interfaces." Nucleic acids research 40.15 (2012): e115-e115. doi: 10.1093/nar/gks596
Licensed under GPL v2 to comply with the primer3 license.
CFLAGS=-std=c99 pip install git+https://github.com/diekhans/PrimerS-JuJu.git
primer3-py needs -std=c99, at least on some systems.
-
available from http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64/
- bigBedNamedItems
- bigBedToBed
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available from http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64/blat/
- gfPcr
- gfServer (if not using an assembly in the UCSC browser)