diff --git a/README.md b/README.md index ac652a9..7ad4fb5 100644 --- a/README.md +++ b/README.md @@ -29,12 +29,10 @@ The "Draw Measurement" plugin then allows you to draw a line with the calibrated 1. Open FIJI 1. Open an image file taken at the desired maginification with a measurment marker. e.g. Open a photo of your micrometer slide taken with your 40x objective 1. Zoom in on the photo to view the micrometer scale -1. Draw a line `ROI` (Region Of Interest) along the calibration measurment feature. e.g. along the micrometer - +1. Draw a line `ROI` (Region Of Interest) along the calibration measurment feature. e.g. along the micrometer 1. Navigate to and select `Analyze > Set Scale` 1. The "Distance in Pixels" will already be set by your line ROI -1. Type in the "Known Distance" from your measurement feature, mine is 5μm - +1. Type in the "Known Distance" from your measurement feature, mine is 5μm 1. Record the resulting "Scale" value, e.g. 31.1716 pixel/unit, where unit is cm, mm, μm, etc 1. The "Scale" value will be used in your `Microscope_Calibrations_user_settings.py` file, so recored both a name and the scale value. e.g: ``` @@ -67,9 +65,8 @@ The "Draw Measurement" plugin then allows you to draw a line with the calibrated 1. Re-start the FIJI application. This will allow the application to register the changes you made to the plugin **note** _for any subsquent changes, you will have to save the file, quit the application and re-open it to see the changes_ 1. Open an image -1. Run `Analyze > Microscope Measurment Tools > Choose Microscope Calibration` and see a pop-up window that shows the new names and calibration values you set in `Microscope_Calibrations_user_settings.py`. - -1. You may also apply the same scale and scale bar to all images you have open, but selecting the checkboxes. Doing so will open another pop-up, where you can see how the scale will look. In the example below, the bar is set to 10μm, white text and placed in the lower right corner +1. Run `Analyze > Microscope Measurment Tools > Choose Microscope Calibration` and see a pop-up window that shows the new names and calibration values you set in `Microscope_Calibrations_user_settings.py`. +1. You may also apply the same scale and scale bar to all images you have open, but selecting the checkboxes. Doing so will open another pop-up, where you can see how the scale will look. In the example below, the bar is set to 10μm, white text and placed in the lower right corner ## 📈 Usage