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Error in binning and derep #559

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srishti8724 opened this issue Oct 29, 2024 · 0 comments
Open

Error in binning and derep #559

srishti8724 opened this issue Oct 29, 2024 · 0 comments

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@srishti8724
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I have 9 soil samples. I am doing analysis of shotgun metagenomic data which near about 25 millions reads in each sample. Now after quality control I have tried megahit for assembly. I have done coassembly and then I have done metabat2, maxbin and concoct but it gives near 180 bins all together. After derep things, It gives hardly 5 bins of good quality. I have searched many literature and all the stuffs but I cant understand what I can do to solve the issue. my project is focusing pesticide degrading gene identification.

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