diff --git a/ha-na-nextstrain/2022-02-23-seasonal-flu-ha-na-reassortment.ipynb b/ha-na-nextstrain/2022-02-23-seasonal-flu-ha-na-reassortment.ipynb
index 1cfc4d14..c2d7647c 100644
--- a/ha-na-nextstrain/2022-02-23-seasonal-flu-ha-na-reassortment.ipynb
+++ b/ha-na-nextstrain/2022-02-23-seasonal-flu-ha-na-reassortment.ipynb
@@ -75,6 +75,7 @@
     "output_mds_png = snakemake.output.HANAFullChartBrushableMDSPNG\n",
     "output_tsne_html = snakemake.output.HANAFullChartBrushableTSNEHTML\n",
     "output_tsne_png = snakemake.output.HANAFullChartBrushableTSNEPNG\n",
+    "output_tsne_mcc_counts_png = snakemake.output.tsne_mcc_counts\n",
     "output_umap_html = snakemake.output.HANAFullChartBrushableUMAPHTML\n",
     "output_umap_png = snakemake.output.HANAFullChartBrushableUMAPPNG\n",
     "output_ha_na_html = snakemake.output.HANAChartHTML\n",
@@ -964,6 +965,158 @@
     "tsne_final_chart.save(output_tsne_png, format=\"png\", scale_factor=2.0)"
    ]
   },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "aacebf2a",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_MCC_counts = embeddings_df.loc[\n",
+    "    (\n",
+    "        (embeddings_df[\"is_internal_node\"] == False) &\n",
+    "        (embeddings_df[\"MCC\"] != \"unassigned\") &\n",
+    "        (embeddings_df[\"t-sne_concatenated_label\"] != -1)\n",
+    "    ),\n",
+    "    [\"MCC\", \"t-sne_concatenated_label\"]\n",
+    "].value_counts().reset_index(name=\"count\")"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "39522c1f",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_MCC_counts[\"MCC_number\"] = tsne_MCC_counts[\"MCC\"].apply(lambda mcc: int(mcc.split(\"_\")[-1]))"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "9e794f98",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_MCC_counts = tsne_MCC_counts.sort_values([\"t-sne_concatenated_label\", \"MCC_number\"])"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "246332c3",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_MCC_counts[\"t-sne_concatenated_label\"] = tsne_MCC_counts[\"t-sne_concatenated_label\"].astype(int).astype(str)"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "3b586e9b",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_MCC_counts"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "cde59df4",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_mcc_counts_chart = alt.Chart(tsne_MCC_counts.query(\"count >= 10\")).mark_circle().encode(\n",
+    "    x=alt.X(\"MCC:N\", title=\"Reassortment group from TreeKnit\", sort={\"field\": \"MCC_number\"}),\n",
+    "    y=alt.Y(\"t-sne_concatenated_label:N\", title=\"Cluster from t-SNE of HA and NA\", sort=None),\n",
+    "    size=\"count:Q\",\n",
+    ")\n",
+    "tsne_mcc_counts_chart"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "6b1ea4e2",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_mcc_counts_chart.save(output_tsne_mcc_counts_png, format=\"png\", scale_factor=2.0)"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "efb96a6b",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_MCC_counts[\"MCC\"].drop_duplicates().shape"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "2a1459d4",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "embeddings_df.head()"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "f3b31327",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_clade_counts = embeddings_df.loc[\n",
+    "    (\n",
+    "        (embeddings_df[\"is_internal_node\"] == False) &\n",
+    "        (embeddings_df[\"t-sne_concatenated_label\"] != -1)\n",
+    "    ),\n",
+    "    [\"clade_membership\", \"t-sne_concatenated_label\"]\n",
+    "].value_counts().reset_index(name=\"count\")"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "c828c993",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_clade_counts[\"t-sne_concatenated_label\"] = tsne_clade_counts[\"t-sne_concatenated_label\"].astype(int).astype(str)"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "2c24b8a0",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "tsne_clade_counts"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "id": "1550932d",
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "alt.Chart(tsne_clade_counts.query(\"count >= 10\")).mark_circle().encode(\n",
+    "    x=\"clade_membership:N\",\n",
+    "    y=\"t-sne_concatenated_label:N\",\n",
+    "    size=\"count:Q\",\n",
+    ")"
+   ]
+  },
   {
    "cell_type": "markdown",
    "id": "ce97a775",
diff --git a/ha-na-nextstrain/Snakefile b/ha-na-nextstrain/Snakefile
index 2cb18a84..d8163509 100644
--- a/ha-na-nextstrain/Snakefile
+++ b/ha-na-nextstrain/Snakefile
@@ -758,6 +758,7 @@ rule seasonal_flu_reassortment_create_notebook_docs:
         HANAFullChartBrushableMDSPNG = "manuscript/figures/flu-2016-2018-ha-na-mds-by-cluster.png",
         HANAFullChartBrushableTSNEHTML = "manuscript/figures/flu-2016-2018-ha-na-tsne-by-cluster.html",
         HANAFullChartBrushableTSNEPNG = "manuscript/figures/flu-2016-2018-ha-na-tsne-by-cluster.png",
+        tsne_mcc_counts = "manuscript/figures/flu-2016-2018-ha-na-tsne-mcc-counts.png",
         HANAFullChartBrushableUMAPHTML = "manuscript/figures/flu-2016-2018-ha-na-umap-by-cluster.html",
         HANAFullChartBrushableUMAPPNG = "manuscript/figures/flu-2016-2018-ha-na-umap-by-cluster.png",
     params: