diff --git a/.github/workflows/python-app.yml b/.github/workflows/python-app.yml index 0bb8d43..b63d89d 100644 --- a/.github/workflows/python-app.yml +++ b/.github/workflows/python-app.yml @@ -28,7 +28,6 @@ jobs: python -m pip install --upgrade pip pip install flake8 pytest if [ -f requirements.txt ]; then pip install -r requirements.txt; fi - python setup.py install - name: Lint with flake8 run: | # stop the build if there are Python syntax errors or undefined names @@ -37,6 +36,6 @@ jobs: flake8 . --count --exit-zero --max-complexity=10 --max-line-length=127 --statistics - name: Test with unittest run: | - cd tests/ + python setup.py install python -m unittest diff --git a/.github/workflows/python-package.yml b/.github/workflows/python-package.yml index cf78424..e7be7b3 100644 --- a/.github/workflows/python-package.yml +++ b/.github/workflows/python-package.yml @@ -29,7 +29,6 @@ jobs: python -m pip install --upgrade pip python -m pip install flake8 pytest if [ -f requirements.txt ]; then pip install -r requirements.txt; fi - python setup.py install - name: Lint with flake8 run: | # stop the build if there are Python syntax errors or undefined names @@ -38,13 +37,13 @@ jobs: flake8 . --count --exit-zero --max-complexity=10 --max-line-length=127 --statistics - name: Test with unittest run: | - cd tests/ + python setup.py install python -m unittest - name: Test commandline tool help run: | ibaqpy --help - name: Test commandline normalization features run: | - rm -rfv tests/PXD003947/PXD003947-peptides-norm.csv - rm -rfv tests/PXD003947/PXD003947-peptides-norm.parquet - ibaqpy features2peptides --parquet tests/PXD003947/PXD003947-feature.parquet --sdrf tests/PXD003947/PXD003947.sdrf.tsv --min_aa 7 --min_unique 2 --remove_ids data/contaminants_ids.tsv --remove_decoy_contaminants --remove_low_frequency_peptides --output tests/PXD003947/PXD003947-peptides-norm.csv --skip_normalization --nmethod median --pnmethod max_min --log2 --save_parquet \ No newline at end of file + rm -rfv tests/example/PXD017834-ibaq-norm.csv + rm -rfv tests/example/PXD017834-peptides-norm.csv + ibaqpy features2peptides --parquet tests/example/feature.parquet --sdrf tests/example/PXD017834-TMT.sdrf.tsv --min_aa 7 --min_unique 2 --remove_ids data/contaminants_ids.tsv --remove_decoy_contaminants --remove_low_frequency_peptides --output tests/example/PXD017834-peptides-norm.csv --skip_normalization --nmethod median --pnmethod max_min --log2 --save_parquet \ No newline at end of file diff --git a/.gitignore b/.gitignore index ed58c0e..5a3dace 100644 --- a/.gitignore +++ b/.gitignore @@ -15,4 +15,5 @@ venv /tests/PXD003947/PXD003947-peptides-norm.csv /tests/PXD003947/PXD003947-peptides-norm.parquet /build/ -/dist/ \ No newline at end of file +/dist/ +/**/__pycache__/ \ No newline at end of file diff --git a/data/__init__.py b/data/__init__.py new file mode 100644 index 0000000..e69de29 diff --git a/ibaqpy/ibaq/peptide_normalization.py b/ibaqpy/ibaq/peptide_normalization.py index f24fbf3..3c5973d 100644 --- a/ibaqpy/ibaq/peptide_normalization.py +++ b/ibaqpy/ibaq/peptide_normalization.py @@ -327,30 +327,30 @@ def low_frequency_peptides(self, percentage=0.2) -> tuple: """Return peptides with low frequency""" f_table = self.parquet_db.sql( """ - SELECT "sequence","protein_accessions",COUNT(DISTINCT sample_accession) as "count" from parquet_db - GROUP BY "sequence","protein_accessions" + SELECT "sequence","pg_accessions",COUNT(DISTINCT sample_accession) as "count" from parquet_db + GROUP BY "sequence","pg_accessions" """ ).df() try: - f_table["protein_accessions"] = f_table["protein_accessions"].apply( + f_table["pg_accessions"] = f_table["pg_accessions"].apply( lambda x: x[0].split("|")[1] ) except IndexError: - f_table["protein_accessions"] = f_table["protein_accessions"].apply( + f_table["pg_accessions"] = f_table["pg_accessions"].apply( lambda x: x[0] ) except Exception as e: print(e) exit( - "Some errors occurred when parsing protein_accessions column in feature parquet!" + "Some errors occurred when parsing pg_accessions column in feature parquet!" ) - f_table.set_index(["sequence", "protein_accessions"], inplace=True) + f_table.set_index(["sequence", "pg_accessions"], inplace=True) f_table.drop( f_table[f_table["count"] >= (percentage * len(self.samples))].index, inplace=True, ) f_table.reset_index(inplace=True) - return tuple(zip(f_table["protein_accessions"], f_table["sequence"])) + return tuple(zip(f_table["pg_accessions"], f_table["sequence"])) @staticmethod def csv2parquet(csv): diff --git a/ibaqpy/ibaq/utils.py b/ibaqpy/ibaq/utils.py index f9cecc1..310c1ab 100644 --- a/ibaqpy/ibaq/utils.py +++ b/ibaqpy/ibaq/utils.py @@ -10,7 +10,7 @@ from sklearn.decomposition import PCA from sklearn.impute import KNNImputer -#from combat.pycombat import pycombat +from combat.pycombat import pycombat logging.basicConfig( format="%(asctime)s [%(funcName)s] - %(message)s", level=logging.DEBUG diff --git a/tests/PXD003947/PXD003947-feature.parquet b/tests/PXD003947/PXD003947-feature.parquet deleted file mode 100644 index 90e84c1..0000000 Binary files a/tests/PXD003947/PXD003947-feature.parquet and /dev/null differ diff --git a/tests/PXD003947/PXD003947.sdrf.tsv b/tests/PXD003947/PXD003947.sdrf.tsv deleted file mode 100644 index fcce355..0000000 --- a/tests/PXD003947/PXD003947.sdrf.tsv +++ /dev/null @@ -1,109 +0,0 @@ -source name characteristics[organism] characteristics[organism part] characteristics[disease] characteristics[ancestry category] characteristics[age] characteristics[developmental stage] characteristics[sex] characteristics[cell line] characteristics[cell type] characteristics[biological replicate] Material Type assay name Technology Type comment[label] comment[data file] comment[file uri] comment[technical replicate] comment[fraction identifier] comment[MS2 mass analyzer] comment[instrument] comment[modification parameters] comment[modification parameters] comment[modification parameters] comment[cleavage agent details] comment[dissociation method] comment[precursor mass tolerance] comment[fragment mass tolerance] factor value[organism part] -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 1 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07437.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07437.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 2 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07439.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07439.raw 1 2 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 3 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07440.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07440.raw 1 3 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 4 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07441.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07441.raw 1 4 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 5 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07442.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07442.raw 1 5 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 6 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07443.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07443.raw 1 6 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 7 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07444.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07444.raw 1 7 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 8 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07445.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07445.raw 1 8 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 9 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07446.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07446.raw 1 9 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 10 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07447.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07447.raw 1 10 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 11 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07448.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07448.raw 1 11 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 12 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07449.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07449.raw 1 12 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 13 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07450.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07450.raw 1 13 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 14 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07451.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07451.raw 1 14 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 15 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07452.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07452.raw 1 15 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 16 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07453.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07453.raw 1 16 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 17 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07454.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07454.raw 1 17 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 18 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07455.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07455.raw 1 18 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 19 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07456.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07456.raw 1 19 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 20 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07457.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07457.raw 1 20 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 21 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07458.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07458.raw 1 21 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 22 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07459.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07459.raw 1 22 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 23 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07460.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07460.raw 1 23 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-1 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 24 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07461.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07461.raw 1 24 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 25 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07559.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07559.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 26 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07567.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07567.raw 1 2 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 27 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07568.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07568.raw 1 3 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 28 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07569.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07569.raw 1 4 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 29 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07570.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07570.raw 1 5 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 30 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07571.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07571.raw 1 6 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 31 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07572.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07572.raw 1 7 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 32 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07573.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07573.raw 1 8 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 33 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07574.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07574.raw 1 9 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 34 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07575.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07575.raw 1 10 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 35 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07576.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07576.raw 1 11 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 36 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07577.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07577.raw 1 12 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 37 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07578.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07578.raw 1 13 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 38 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07579.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07579.raw 1 14 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 39 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07580.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07580.raw 1 15 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 40 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07581.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07581.raw 1 16 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 41 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07582.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07582.raw 1 17 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 42 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07583.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07583.raw 1 18 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 43 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07584.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07584.raw 1 19 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 44 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07585.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07585.raw 1 20 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 45 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07586.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07586.raw 1 21 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 46 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07587.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07587.raw 1 22 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 47 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07588.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07588.raw 1 23 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 48 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07589.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07589.raw 1 24 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 49 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07590.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07590.raw 1 25 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 50 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07591.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07591.raw 1 26 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 51 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07592.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07592.raw 1 27 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 52 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07593.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07593.raw 1 28 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-2 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 53 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QE07594.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QE07594.raw 1 29 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 54 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006847.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006847.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 55 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006848.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006848.raw 1 2 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 56 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006849.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006849.raw 1 3 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 57 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006850.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006850.raw 1 4 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 58 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006851.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006851.raw 1 5 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 59 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006852.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006852.raw 1 6 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 60 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006853.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006853.raw 1 7 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 61 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006854.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006854.raw 1 8 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 62 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006855.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006855.raw 1 9 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 63 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006856.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006856.raw 1 10 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 64 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006857.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006857.raw 1 11 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 65 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006860.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006860.raw 1 12 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 66 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006861.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006861.raw 1 13 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 67 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006862.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006862.raw 1 14 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 68 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006863.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006863.raw 1 15 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 69 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006864.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006864.raw 1 16 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 70 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006865.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006865.raw 1 17 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 71 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006866.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006866.raw 1 18 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 72 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006867.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006867.raw 1 19 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-3 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 73 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006868.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006868.raw 1 20 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 74 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006872.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006872.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 75 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006873.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006873.raw 1 2 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 76 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006874.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006874.raw 1 3 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 77 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006875.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006875.raw 1 4 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 78 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006876.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006876.raw 1 5 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 79 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006877.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006877.raw 1 6 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 80 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006878.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006878.raw 1 7 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 81 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006879.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006879.raw 1 8 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 82 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006880.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006880.raw 1 9 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 83 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006881.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006881.raw 1 10 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 84 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006884.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006884.raw 1 11 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 85 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006885.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006885.raw 1 12 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 86 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006886.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006886.raw 1 13 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 87 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006887.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006887.raw 1 14 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 88 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006888.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006888.raw 1 15 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 89 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006889.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006889.raw 1 16 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 90 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006890.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006890.raw 1 17 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 91 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006891.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006891.raw 1 18 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 92 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006894.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006894.raw 1 19 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-4 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 93 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEx2_006895.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEx2_006895.raw 1 20 AC=MS:1000484; NT=Orbitrap NT=Q Exactive;AC=MS:1001911 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-5 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 94 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA151114_03.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA151114_03.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-6 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 95 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA151114_05.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA151114_05.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-7 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 96 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA151114_07.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA151114_07.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-8 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 97 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA151114_09.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA151114_09.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-9 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 98 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA151119_89.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA151119_89.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 100 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_03.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_03.raw 1 1 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 101 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_05.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_05.raw 1 2 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 102 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_07.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_07.raw 1 3 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 103 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_11.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_11.raw 1 4 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 104 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_32.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_32.raw 1 5 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 105 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_34.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_34.raw 1 6 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 106 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_36.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_36.raw 1 7 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 107 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_38.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_38.raw 1 8 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 108 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_40.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_40.raw 1 9 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon -PXD003947-Sample-11 Homo sapiens Spermatozoon normal not available not available not available not available not applicable not applicable 1 cell run 109 proteomic profiling by mass spectrometry AC=MS:1002038;NT=label free sample QEMMA160105_42.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003947/QEMMA160105_42.raw 1 10 AC=MS:1000484; NT=Orbitrap NT=Q Exactive Plus;AC=MS:1002634 NT=Acetyl;AC=UNIMOD:1;PP=Protein N-term;MT=Variable NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 NT=Carbamidomethyl;AC=UNIMOD:4;TA=C;MT=Fixed NT=Trypsin;AC=MS:1001251 AC=MS:1000422;NT=HCD 5 ppm 20 ppm Spermatozoon diff --git a/tests/__init__.py b/tests/__init__.py new file mode 100644 index 0000000..e69de29 diff --git a/tests/common.py b/tests/common.py new file mode 100644 index 0000000..1df635b --- /dev/null +++ b/tests/common.py @@ -0,0 +1,7 @@ +from pathlib import Path + +TEST_DATA_ROOT = Path(__file__).parent / "example" + +def datafile(path: str): + path = str(path) + return str(TEST_DATA_ROOT / path) \ No newline at end of file diff --git a/tests/PXD003947/Homo-sapiens-uniprot-reviewed-contaminants-decoy-202210.fasta b/tests/example/Homo-sapiens-uniprot-reviewed-contaminants-decoy-202210.fasta similarity index 100% rename from tests/PXD003947/Homo-sapiens-uniprot-reviewed-contaminants-decoy-202210.fasta rename to tests/example/Homo-sapiens-uniprot-reviewed-contaminants-decoy-202210.fasta diff --git a/tests/example/PXD017834-TMT.sdrf.tsv b/tests/example/PXD017834-TMT.sdrf.tsv new file mode 100644 index 0000000..871390a --- /dev/null +++ b/tests/example/PXD017834-TMT.sdrf.tsv @@ -0,0 +1,109 @@ +source name characteristics[organism] characteristics[organism part] characteristics[cell type] characteristics[ancestry category] characteristics[age] characteristics[sex] characteristics[developmental stage] characteristics[disease] characteristics[individual] characteristics[biological replicate] characteristics[depletion] assay name Technology Type comment[data file] comment[file uri] comment[fraction identifier] comment[label] comment[technical replicate] comment[cleavage agent details] comment[instrument] comment[modification parameters] comment[modification parameters] comment[modification parameters] comment[modification parameters] comment[precursor mass tolerance] comment[fragment mass tolerance] factor value[organism part] +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 1 proteomic profiling by mass spectrometer TMT_R1F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F1.raw 1 TMT126 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 1 proteomic profiling by mass spectrometer TMT_R1F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F1.raw 1 TMT127 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 1 proteomic profiling by mass spectrometer TMT_R1F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F1.raw 1 TMT128 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 1 proteomic profiling by mass spectrometer TMT_R1F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F1.raw 1 TMT129 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 1 proteomic profiling by mass spectrometer TMT_R1F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F1.raw 1 TMT130 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 1 proteomic profiling by mass spectrometer TMT_R1F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F1.raw 1 TMT131 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 2 proteomic profiling by mass spectrometer TMT_R1F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F2.raw 2 TMT126 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 2 proteomic profiling by mass spectrometer TMT_R1F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F2.raw 2 TMT127 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 2 proteomic profiling by mass spectrometer TMT_R1F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F2.raw 2 TMT128 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 2 proteomic profiling by mass spectrometer TMT_R1F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F2.raw 2 TMT129 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 2 proteomic profiling by mass spectrometer TMT_R1F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F2.raw 2 TMT130 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 2 proteomic profiling by mass spectrometer TMT_R1F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F2.raw 2 TMT131 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 3 proteomic profiling by mass spectrometer TMT_R1F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F3.raw 3 TMT126 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 3 proteomic profiling by mass spectrometer TMT_R1F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F3.raw 3 TMT127 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 3 proteomic profiling by mass spectrometer TMT_R1F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F3.raw 3 TMT128 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 3 proteomic profiling by mass spectrometer TMT_R1F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F3.raw 3 TMT129 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 3 proteomic profiling by mass spectrometer TMT_R1F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F3.raw 3 TMT130 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 3 proteomic profiling by mass spectrometer TMT_R1F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F3.raw 3 TMT131 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 4 proteomic profiling by mass spectrometer TMT_R1F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F4.raw 4 TMT126 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 4 proteomic profiling by mass spectrometer TMT_R1F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F4.raw 4 TMT127 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 4 proteomic profiling by mass spectrometer TMT_R1F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F4.raw 4 TMT128 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 4 proteomic profiling by mass spectrometer TMT_R1F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F4.raw 4 TMT129 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 4 proteomic profiling by mass spectrometer TMT_R1F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F4.raw 4 TMT130 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 4 proteomic profiling by mass spectrometer TMT_R1F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F4.raw 4 TMT131 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 5 proteomic profiling by mass spectrometer TMT_R1F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F5.raw 5 TMT126 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 5 proteomic profiling by mass spectrometer TMT_R1F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F5.raw 5 TMT127 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 5 proteomic profiling by mass spectrometer TMT_R1F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F5.raw 5 TMT128 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 5 proteomic profiling by mass spectrometer TMT_R1F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F5.raw 5 TMT129 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 5 proteomic profiling by mass spectrometer TMT_R1F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F5.raw 5 TMT130 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 5 proteomic profiling by mass spectrometer TMT_R1F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F5.raw 5 TMT131 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 6 proteomic profiling by mass spectrometer TMT_R1F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F6.raw 6 TMT126 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 6 proteomic profiling by mass spectrometer TMT_R1F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F6.raw 6 TMT127 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 6 proteomic profiling by mass spectrometer TMT_R1F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F6.raw 6 TMT128 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 6 proteomic profiling by mass spectrometer TMT_R1F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F6.raw 6 TMT129 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 6 proteomic profiling by mass spectrometer TMT_R1F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F6.raw 6 TMT130 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 6 proteomic profiling by mass spectrometer TMT_R1F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R1F6.raw 6 TMT131 1 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 7 proteomic profiling by mass spectrometer TMT_R2F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F1.raw 1 TMT126 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 7 proteomic profiling by mass spectrometer TMT_R2F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F1.raw 1 TMT127 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 7 proteomic profiling by mass spectrometer TMT_R2F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F1.raw 1 TMT128 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 7 proteomic profiling by mass spectrometer TMT_R2F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F1.raw 1 TMT129 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 7 proteomic profiling by mass spectrometer TMT_R2F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F1.raw 1 TMT130 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 7 proteomic profiling by mass spectrometer TMT_R2F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F1.raw 1 TMT131 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 8 proteomic profiling by mass spectrometer TMT_R2F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F2.raw 2 TMT126 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 8 proteomic profiling by mass spectrometer TMT_R2F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F2.raw 2 TMT127 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 8 proteomic profiling by mass spectrometer TMT_R2F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F2.raw 2 TMT128 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 8 proteomic profiling by mass spectrometer TMT_R2F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F2.raw 2 TMT129 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 8 proteomic profiling by mass spectrometer TMT_R2F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F2.raw 2 TMT130 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 8 proteomic profiling by mass spectrometer TMT_R2F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F2.raw 2 TMT131 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 9 proteomic profiling by mass spectrometer TMT_R2F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F3.raw 3 TMT126 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 9 proteomic profiling by mass spectrometer TMT_R2F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F3.raw 3 TMT127 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 9 proteomic profiling by mass spectrometer TMT_R2F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F3.raw 3 TMT128 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 9 proteomic profiling by mass spectrometer TMT_R2F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F3.raw 3 TMT129 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 9 proteomic profiling by mass spectrometer TMT_R2F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F3.raw 3 TMT130 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 9 proteomic profiling by mass spectrometer TMT_R2F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F3.raw 3 TMT131 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 10 proteomic profiling by mass spectrometer TMT_R2F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F4.raw 4 TMT126 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 10 proteomic profiling by mass spectrometer TMT_R2F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F4.raw 4 TMT127 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 10 proteomic profiling by mass spectrometer TMT_R2F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F4.raw 4 TMT128 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 10 proteomic profiling by mass spectrometer TMT_R2F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F4.raw 4 TMT129 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 10 proteomic profiling by mass spectrometer TMT_R2F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F4.raw 4 TMT130 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 10 proteomic profiling by mass spectrometer TMT_R2F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F4.raw 4 TMT131 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 11 proteomic profiling by mass spectrometer TMT_R2F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F5.raw 5 TMT126 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 11 proteomic profiling by mass spectrometer TMT_R2F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F5.raw 5 TMT127 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 11 proteomic profiling by mass spectrometer TMT_R2F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F5.raw 5 TMT128 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 11 proteomic profiling by mass spectrometer TMT_R2F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F5.raw 5 TMT129 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 11 proteomic profiling by mass spectrometer TMT_R2F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F5.raw 5 TMT130 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 11 proteomic profiling by mass spectrometer TMT_R2F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F5.raw 5 TMT131 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 12 proteomic profiling by mass spectrometer TMT_R2F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F6.raw 6 TMT126 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 12 proteomic profiling by mass spectrometer TMT_R2F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F6.raw 6 TMT127 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 12 proteomic profiling by mass spectrometer TMT_R2F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F6.raw 6 TMT128 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 12 proteomic profiling by mass spectrometer TMT_R2F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F6.raw 6 TMT129 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 12 proteomic profiling by mass spectrometer TMT_R2F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F6.raw 6 TMT130 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 12 proteomic profiling by mass spectrometer TMT_R2F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R2F6.raw 6 TMT131 2 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 13 proteomic profiling by mass spectrometer TMT_R3F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F1.raw 1 TMT126 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 13 proteomic profiling by mass spectrometer TMT_R3F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F1.raw 1 TMT127 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 13 proteomic profiling by mass spectrometer TMT_R3F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F1.raw 1 TMT128 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 13 proteomic profiling by mass spectrometer TMT_R3F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F1.raw 1 TMT129 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 13 proteomic profiling by mass spectrometer TMT_R3F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F1.raw 1 TMT130 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 13 proteomic profiling by mass spectrometer TMT_R3F1.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F1.raw 1 TMT131 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 14 proteomic profiling by mass spectrometer TMT_R3F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F2.raw 2 TMT126 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 14 proteomic profiling by mass spectrometer TMT_R3F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F2.raw 2 TMT127 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 14 proteomic profiling by mass spectrometer TMT_R3F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F2.raw 2 TMT128 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 14 proteomic profiling by mass spectrometer TMT_R3F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F2.raw 2 TMT129 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 14 proteomic profiling by mass spectrometer TMT_R3F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F2.raw 2 TMT130 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 14 proteomic profiling by mass spectrometer TMT_R3F2.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F2.raw 2 TMT131 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 15 proteomic profiling by mass spectrometer TMT_R3F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F3.raw 3 TMT126 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 15 proteomic profiling by mass spectrometer TMT_R3F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F3.raw 3 TMT127 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 15 proteomic profiling by mass spectrometer TMT_R3F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F3.raw 3 TMT128 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 15 proteomic profiling by mass spectrometer TMT_R3F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F3.raw 3 TMT129 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 15 proteomic profiling by mass spectrometer TMT_R3F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F3.raw 3 TMT130 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 15 proteomic profiling by mass spectrometer TMT_R3F3.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F3.raw 3 TMT131 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 16 proteomic profiling by mass spectrometer TMT_R3F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F4.raw 4 TMT126 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 16 proteomic profiling by mass spectrometer TMT_R3F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F4.raw 4 TMT127 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 16 proteomic profiling by mass spectrometer TMT_R3F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F4.raw 4 TMT128 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 16 proteomic profiling by mass spectrometer TMT_R3F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F4.raw 4 TMT129 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 16 proteomic profiling by mass spectrometer TMT_R3F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F4.raw 4 TMT130 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 16 proteomic profiling by mass spectrometer TMT_R3F4.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F4.raw 4 TMT131 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 17 proteomic profiling by mass spectrometer TMT_R3F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F5.raw 5 TMT126 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 17 proteomic profiling by mass spectrometer TMT_R3F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F5.raw 5 TMT127 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 17 proteomic profiling by mass spectrometer TMT_R3F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F5.raw 5 TMT128 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 17 proteomic profiling by mass spectrometer TMT_R3F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F5.raw 5 TMT129 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 17 proteomic profiling by mass spectrometer TMT_R3F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F5.raw 5 TMT130 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 17 proteomic profiling by mass spectrometer TMT_R3F5.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F5.raw 5 TMT131 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-1 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 1 depletion run 18 proteomic profiling by mass spectrometer TMT_R3F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F6.raw 6 TMT126 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-2 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 2 depletion run 18 proteomic profiling by mass spectrometer TMT_R3F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F6.raw 6 TMT127 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-3 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 3 depletion run 18 proteomic profiling by mass spectrometer TMT_R3F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F6.raw 6 TMT128 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-4 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 4 depletion run 18 proteomic profiling by mass spectrometer TMT_R3F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F6.raw 6 TMT129 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-5 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 5 depletion run 18 proteomic profiling by mass spectrometer TMT_R3F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F6.raw 6 TMT130 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma +PXD017834-Sample-6 Homo Sapiens Blood plasma not available not available not available not available not available not available not available 6 depletion run 18 proteomic profiling by mass spectrometer TMT_R3F6.raw ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017834/TMT_R3F6.raw 6 TMT131 3 NT=Trypsin; AC=MS:1001251 NT=Orbitrap Fusion;AC=MS:1002416 NT=TMT6plex;TA=K;MT=Variable;AC=UNIMOD:737 NT=TMT6plex;PP=Any N-term;MT=Variable;AC=UNIMOD:737 NT=Carbamidomethyl;TA=C;MT=fixed;AC=UNIMOD:4 NT=Oxidation;MT=variable;TA=M;AC=UNIMOD:35 10 ppm 0.05 Da Blood Plasma \ No newline at end of file diff --git a/tests/example/feature.parquet b/tests/example/feature.parquet new file mode 100644 index 0000000..f9f7f6d Binary files /dev/null and b/tests/example/feature.parquet differ diff --git a/tests/test_ibaqpy.py b/tests/test_ibaqpy.py index 903101f..ba993d7 100644 --- a/tests/test_ibaqpy.py +++ b/tests/test_ibaqpy.py @@ -1,41 +1,40 @@ from unittest import TestCase - from ibaqpy.ibaq.peptide_normalization import peptide_normalization from ibaqpy.ibaq.compute_ibaq import ibaq_compute - +from .common import datafile class TestIbaqpy(TestCase): def test_feature_assembly(self): args = { - "parquet": __package__ + "PXD003947/PXD003947-feature.parquet", - "sdrf": __package__ + "PXD003947/PXD003947.sdrf.tsv", + "parquet": datafile("feature.parquet"), + "sdrf": datafile("PXD017834-TMT.sdrf.tsv"), "min_aa": 7, "min_unique": 2, - "remove_ids": __package__ + "../data/contaminants_ids.tsv", + "remove_ids": None, "remove_decoy_contaminants": True, "remove_low_frequency_peptides": True, - "output": __package__ + "PXD003947/PXD003947-peptides-norm.csv", + "output": datafile("PXD017834-peptides-norm.csv"), "skip_normalization": False, "nmethod": "median", "pnmethod": "max_min", "log2": True, "save_parquet": True, } + print(__package__) print(args) peptide_normalization(**args) def test_ibaq_compute(self): args = { - "fasta": __package__ - + "PXD003947/Homo-sapiens-uniprot-reviewed-contaminants-decoy-202210.fasta", - "peptides": __package__ + "PXD003947/PXD003947-peptides-norm.csv", + "fasta": datafile("Homo-sapiens-uniprot-reviewed-contaminants-decoy-202210.fasta"), + "peptides": datafile("PXD017834-peptides-norm.csv"), "enzyme": "Trypsin", "normalize": True, "min_aa": 7, "max_aa": 30, - "output": __package__ + "PXD003947/PXD003947-ibaq-norm.csv", + "output": datafile("PXD017834-ibaq-norm.csv"), "verbose": True, - "qc_report": __package__ + "PXD003947/IBAQ-QCprofile.pdf", + "qc_report": datafile("IBAQ-QCprofile.pdf"), } print(args) ibaq_compute(**args)