diff --git a/pyiceberg/io/pyarrow.py b/pyiceberg/io/pyarrow.py
index d2432e80e7..f554f35369 100644
--- a/pyiceberg/io/pyarrow.py
+++ b/pyiceberg/io/pyarrow.py
@@ -362,6 +362,12 @@ def _initialize_fs(self, scheme: str, netloc: Optional[str] = None) -> FileSyste
                 "region": get_first_property_value(self.properties, S3_REGION, AWS_REGION),
             }
 
+            # Override the default s3.region if netloc(bucket) resolves to a different region
+            try:
+                client_kwargs["region"] = resolve_s3_region(netloc)
+            except (OSError, TypeError):
+                pass
+
             if proxy_uri := self.properties.get(S3_PROXY_URI):
                 client_kwargs["proxy_options"] = proxy_uri
 
@@ -377,12 +383,6 @@ def _initialize_fs(self, scheme: str, netloc: Optional[str] = None) -> FileSyste
             if force_virtual_addressing := self.properties.get(S3_FORCE_VIRTUAL_ADDRESSING):
                 client_kwargs["force_virtual_addressing"] = property_as_bool(self.properties, force_virtual_addressing, False)
 
-            # Override the default s3.region if netloc(bucket) resolves to a different region
-            try:
-                client_kwargs["region"] = resolve_s3_region(netloc)
-            except (OSError, TypeError):
-                pass
-
             return S3FileSystem(**client_kwargs)
         elif scheme in ("hdfs", "viewfs"):
             from pyarrow.fs import HadoopFileSystem