diff --git a/.gitignore b/.gitignore
index 25a4804ab3..88e3b74c62 100644
--- a/.gitignore
+++ b/.gitignore
@@ -26,3 +26,4 @@ tools/test-schema/node_modules
 site
 .DS_Store
 src/molecule/_version.py
+.vscode
diff --git a/src/molecule/test/b_functional/test_command.py b/src/molecule/test/b_functional/test_command.py
index 8e86d87c86..b87ca05fdb 100644
--- a/src/molecule/test/b_functional/test_command.py
+++ b/src/molecule/test/b_functional/test_command.py
@@ -20,6 +20,7 @@
 from __future__ import annotations
 
 import os
+import subprocess
 
 import pytest
 from pytest import FixtureRequest
@@ -336,6 +337,39 @@ def test_sample_collection() -> None:
     )
 
 
+def test_sample_collection_venv_with_gitignore() -> None:
+    op = subprocess.run(
+        "python -m venv venv \
+        && source venv/bin/activate \
+        && pip install ../../../../../ \
+        && molecule list \
+        && deactivate \
+        && rm -rf source venv",
+        shell=True,
+        cwd="src/molecule/test/resources/sample-collection-venv-with-gitignore",
+        check=True,
+    )
+    assert op.returncode == 0
+
+
+def test_sample_collection_venv_without_gitignore() -> None:
+    with pytest.raises(subprocess.CalledProcessError) as e:
+        subprocess.run(
+            "python -m venv venv \
+            && source venv/bin/activate \
+            && pip install ../../../../../ \
+            && molecule list \
+            && deactivate \
+            && rm -rf source venv",
+            shell=True,
+            cwd="src/molecule/test/resources/sample-collection-venv-without-gitignore",
+            check=True,
+        )
+        assert e.value.returncode == 1
+        assert "The scenario config file" in e.value.stderr
+        assert "has been modified since the scenario was created" in e.value.stderr
+
+
 def test_podman() -> None:
     assert (
         run_command(
diff --git a/src/molecule/test/resources/sample-collection-venv-with-gitignore/.gitignore b/src/molecule/test/resources/sample-collection-venv-with-gitignore/.gitignore
new file mode 100644
index 0000000000..ca944a00d1
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-with-gitignore/.gitignore
@@ -0,0 +1,9 @@
+# Environments
+.env
+.venv
+env/
+venv/
+ENV/
+env.bak/
+venv.bak/
+source/
diff --git a/src/molecule/test/resources/sample-collection-venv-with-gitignore/extensions/molecule/default/converge.yml b/src/molecule/test/resources/sample-collection-venv-with-gitignore/extensions/molecule/default/converge.yml
new file mode 100644
index 0000000000..73f7cfb3c3
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-with-gitignore/extensions/molecule/default/converge.yml
@@ -0,0 +1,7 @@
+---
+- name: Converge
+  hosts: localhost
+  tasks:
+    - name: "Include sample role from current collection"
+      ansible.builtin.include_role:
+        name: acme.goodies.get_rich
diff --git a/src/molecule/test/resources/sample-collection-venv-with-gitignore/extensions/molecule/default/molecule.yml b/src/molecule/test/resources/sample-collection-venv-with-gitignore/extensions/molecule/default/molecule.yml
new file mode 100644
index 0000000000..1cec4936a1
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-with-gitignore/extensions/molecule/default/molecule.yml
@@ -0,0 +1,11 @@
+---
+dependency:
+  name: galaxy
+driver:
+  name: default
+platforms:
+  - name: instance
+provisioner:
+  name: ansible
+verifier:
+  name: ansible
diff --git a/src/molecule/test/resources/sample-collection-venv-with-gitignore/galaxy.yml b/src/molecule/test/resources/sample-collection-venv-with-gitignore/galaxy.yml
new file mode 100644
index 0000000000..2a7e1164d3
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-with-gitignore/galaxy.yml
@@ -0,0 +1,31 @@
+name: goodies
+namespace: acme
+version: 1.0.0
+readme: README.md
+authors:
+  - Red Hat
+description: Acme Goodies Collection
+build_ignore:
+  - "*.egg-info"
+  - .DS_Store
+  - .eggs
+  - .gitignore
+  - .mypy_cache
+  - .pytest_cache
+  - .stestr
+  - .stestr.conf
+  - .tox
+  - .vscode
+  - MANIFEST.in
+  - build
+  - dist
+  - doc
+  - report.html
+  - setup.cfg
+  - setup.py
+  - "tests/unit/*.*"
+  - README.rst
+  - tox.ini
+
+repository: https://opendev.org/openstack/tripleo-repos
+license_file: LICENSE
diff --git a/src/molecule/test/resources/sample-collection-venv-with-gitignore/roles/get_rich/tasks/main.yml b/src/molecule/test/resources/sample-collection-venv-with-gitignore/roles/get_rich/tasks/main.yml
new file mode 100644
index 0000000000..cf8063a148
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-with-gitignore/roles/get_rich/tasks/main.yml
@@ -0,0 +1,3 @@
+- name: "some task inside foo.bar collection"
+  ansible.builtin.debug:
+    msg: "hello world!"
diff --git a/src/molecule/test/resources/sample-collection-venv-without-gitignore/extensions/molecule/default/converge.yml b/src/molecule/test/resources/sample-collection-venv-without-gitignore/extensions/molecule/default/converge.yml
new file mode 100644
index 0000000000..73f7cfb3c3
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-without-gitignore/extensions/molecule/default/converge.yml
@@ -0,0 +1,7 @@
+---
+- name: Converge
+  hosts: localhost
+  tasks:
+    - name: "Include sample role from current collection"
+      ansible.builtin.include_role:
+        name: acme.goodies.get_rich
diff --git a/src/molecule/test/resources/sample-collection-venv-without-gitignore/extensions/molecule/default/molecule.yml b/src/molecule/test/resources/sample-collection-venv-without-gitignore/extensions/molecule/default/molecule.yml
new file mode 100644
index 0000000000..1cec4936a1
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-without-gitignore/extensions/molecule/default/molecule.yml
@@ -0,0 +1,11 @@
+---
+dependency:
+  name: galaxy
+driver:
+  name: default
+platforms:
+  - name: instance
+provisioner:
+  name: ansible
+verifier:
+  name: ansible
diff --git a/src/molecule/test/resources/sample-collection-venv-without-gitignore/galaxy.yml b/src/molecule/test/resources/sample-collection-venv-without-gitignore/galaxy.yml
new file mode 100644
index 0000000000..2a7e1164d3
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-without-gitignore/galaxy.yml
@@ -0,0 +1,31 @@
+name: goodies
+namespace: acme
+version: 1.0.0
+readme: README.md
+authors:
+  - Red Hat
+description: Acme Goodies Collection
+build_ignore:
+  - "*.egg-info"
+  - .DS_Store
+  - .eggs
+  - .gitignore
+  - .mypy_cache
+  - .pytest_cache
+  - .stestr
+  - .stestr.conf
+  - .tox
+  - .vscode
+  - MANIFEST.in
+  - build
+  - dist
+  - doc
+  - report.html
+  - setup.cfg
+  - setup.py
+  - "tests/unit/*.*"
+  - README.rst
+  - tox.ini
+
+repository: https://opendev.org/openstack/tripleo-repos
+license_file: LICENSE
diff --git a/src/molecule/test/resources/sample-collection-venv-without-gitignore/roles/get_rich/tasks/main.yml b/src/molecule/test/resources/sample-collection-venv-without-gitignore/roles/get_rich/tasks/main.yml
new file mode 100644
index 0000000000..cf8063a148
--- /dev/null
+++ b/src/molecule/test/resources/sample-collection-venv-without-gitignore/roles/get_rich/tasks/main.yml
@@ -0,0 +1,3 @@
+- name: "some task inside foo.bar collection"
+  ansible.builtin.debug:
+    msg: "hello world!"