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Is normalization necessary for differential analysis? #48

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xiaowenchenjax opened this issue Feb 3, 2023 · 0 comments
Open

Is normalization necessary for differential analysis? #48

xiaowenchenjax opened this issue Feb 3, 2023 · 0 comments

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@xiaowenchenjax
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Dear Developer,
I have a question about normalization, I want to identify different analysis for lipidomics data,
when I used PQN to normalize my data, I got very limited different lipids in case vs control.

  1. Someone suggested me I can use the raw data, is it correct? I am quite new for lipidomics data.

  2. In each dataset, only ~70 lipids, I am thinking about using p value not adjusted p value to select different lipids. Is my thought correct?

Thanks.
Happy Good weekend.
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