How does sequencing depth and cell composition affect RSS outcome?

[Jiayi-Zheng]

Hello, I'm doing comparison on a control and a treatment scRNA-seq dataset, however, the control dataset has a significant lower sequencing depth in comparison to the treatment dataset due to some technical issue at the time of sequencing. I wonder how would that affect the result?

Meanwhile, how does cell type composition in different dataset affect RSS? For example, if I want to compare the top TF in a celltype across different datasets, however, different datasets have somewhat different composition. Would that make this RSS ranking incomparable?

Thank you!