diff --git a/lib/compat/wordpress-6.1/class-wp-theme-json-6-1.php b/lib/compat/wordpress-6.1/class-wp-theme-json-6-1.php
index 68e0dd32187af..c6c41f35ecfc6 100644
--- a/lib/compat/wordpress-6.1/class-wp-theme-json-6-1.php
+++ b/lib/compat/wordpress-6.1/class-wp-theme-json-6-1.php
@@ -825,10 +825,20 @@ function( $pseudo_selector ) use ( $selector ) {
 		if ( static::ROOT_BLOCK_SELECTOR === $selector ) {
 
 			if ( $use_root_padding ) {
+				// Top and bottom padding are applied to the outer block container.
 				$block_rules .= '.wp-site-blocks { padding-top: var(--wp--style--root--padding-top); padding-bottom: var(--wp--style--root--padding-bottom); }';
+				// Right and left padding are applied to the first container with `.has-global-padding` class.
 				$block_rules .= '.has-global-padding { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }';
+				// Nested containers with `.has-global-padding` class do not get padding.
+				$block_rules .= '.has-global-padding :where(.has-global-padding) { padding-right: 0; padding-left: 0; }';
+				// Alignfull children of the container with left and right padding have negative margins so they can still be full width.
 				$block_rules .= '.has-global-padding > .alignfull { margin-right: calc(var(--wp--style--root--padding-right) * -1); margin-left: calc(var(--wp--style--root--padding-left) * -1); }';
-				$block_rules .= '.has-global-padding > .alignfull > :where([class*="wp-block-"]:not(.alignfull):not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }';
+				// The above rule is negated for alignfull children of nested containers.
+				$block_rules .= '.has-global-padding :where(.has-global-padding) > .alignfull { margin-right: 0; margin-left: 0; }';
+				// Some of the children of alignfull blocks without content width should also get padding: text blocks and non-alignfull container blocks.
+				$block_rules .= '.has-global-padding > .alignfull:where(:not(.has-global-padding)) > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }';
+				// The above rule also has to be negated for blocks inside nested `.has-global-padding` blocks.
+				$block_rules .= '.has-global-padding :where(.has-global-padding) > .alignfull:where(:not(.has-global-padding)) > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: 0; padding-left: 0; }';
 			}
 
 			$block_rules .= '.wp-site-blocks > .alignleft { float: left; margin-right: 2em; }';
diff --git a/packages/edit-site/src/components/global-styles/use-global-styles-output.js b/packages/edit-site/src/components/global-styles/use-global-styles-output.js
index 831ea5aa23ba7..189fb3ebbb258 100644
--- a/packages/edit-site/src/components/global-styles/use-global-styles-output.js
+++ b/packages/edit-site/src/components/global-styles/use-global-styles-output.js
@@ -605,8 +605,13 @@ export const toStyles = (
 	}
 
 	if ( useRootPaddingAlign ) {
-		ruleset +=
-			'padding-right: 0; padding-left: 0; padding-top: var(--wp--style--root--padding-top); padding-bottom: var(--wp--style--root--padding-bottom) } .has-global-padding { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); } .has-global-padding > .alignfull { margin-right: calc(var(--wp--style--root--padding-right) * -1); margin-left: calc(var(--wp--style--root--padding-left) * -1); } .has-global-padding > .alignfull > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left);';
+		ruleset += `padding-right: 0; padding-left: 0; padding-top: var(--wp--style--root--padding-top); padding-bottom: var(--wp--style--root--padding-bottom) } 
+			.has-global-padding { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); } 
+			.has-global-padding :where(.has-global-padding) { padding-right: 0; padding-left: 0; } 
+			.has-global-padding > .alignfull { margin-right: calc(var(--wp--style--root--padding-right) * -1); margin-left: calc(var(--wp--style--root--padding-left) * -1); } 
+			.has-global-padding :where(.has-global-padding) > .alignfull { margin-right: 0; margin-left: 0; } 
+			.has-global-padding > .alignfull:where(:not(.has-global-padding)) > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); } 
+			.has-global-padding :where(.has-global-padding) > .alignfull:where(:not(.has-global-padding)) > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: 0; padding-left: 0;`;
 	}
 
 	ruleset += '}';
diff --git a/phpunit/class-wp-theme-json-test.php b/phpunit/class-wp-theme-json-test.php
index ca1748e620755..a9e5eb14f8df7 100644
--- a/phpunit/class-wp-theme-json-test.php
+++ b/phpunit/class-wp-theme-json-test.php
@@ -1149,7 +1149,7 @@ function test_get_styles_for_block_with_padding_aware_alignments() {
 			'selector' => 'body',
 		);
 
-		$expected = 'body{--wp--style--root--padding-top: 10px;--wp--style--root--padding-right: 12px;--wp--style--root--padding-bottom: 10px;--wp--style--root--padding-left: 12px;}body { margin: 0;}.wp-site-blocks { padding-top: var(--wp--style--root--padding-top); padding-bottom: var(--wp--style--root--padding-bottom); }.has-global-padding { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }.has-global-padding > .alignfull { margin-right: calc(var(--wp--style--root--padding-right) * -1); margin-left: calc(var(--wp--style--root--padding-left) * -1); }.has-global-padding > .alignfull > :where([class*="wp-block-"]:not(.alignfull):not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }.wp-site-blocks > .alignleft { float: left; margin-right: 2em; }.wp-site-blocks > .alignright { float: right; margin-left: 2em; }.wp-site-blocks > .aligncenter { justify-content: center; margin-left: auto; margin-right: auto; }';
+		$expected = 'body{--wp--style--root--padding-top: 10px;--wp--style--root--padding-right: 12px;--wp--style--root--padding-bottom: 10px;--wp--style--root--padding-left: 12px;}body { margin: 0;}.wp-site-blocks { padding-top: var(--wp--style--root--padding-top); padding-bottom: var(--wp--style--root--padding-bottom); }.has-global-padding { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }.has-global-padding :where(.has-global-padding) { padding-right: 0; padding-left: 0; }.has-global-padding > .alignfull { margin-right: calc(var(--wp--style--root--padding-right) * -1); margin-left: calc(var(--wp--style--root--padding-left) * -1); }.has-global-padding :where(.has-global-padding) > .alignfull { margin-right: 0; margin-left: 0; }.has-global-padding > .alignfull:where(:not(.has-global-padding)) > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: var(--wp--style--root--padding-right); padding-left: var(--wp--style--root--padding-left); }.has-global-padding :where(.has-global-padding) > .alignfull:where(:not(.has-global-padding)) > :where([class*="wp-block-"]:not(.alignfull):not([class*="__"]),p,h1,h2,h3,h4,h5,h6,ul,ol) { padding-right: 0; padding-left: 0; }.wp-site-blocks > .alignleft { float: left; margin-right: 2em; }.wp-site-blocks > .alignright { float: right; margin-left: 2em; }.wp-site-blocks > .aligncenter { justify-content: center; margin-left: auto; margin-right: auto; }';
 		$this->assertEquals( $expected, $theme_json->get_styles_for_block( $metadata ) );
 	}