GPR curation for Transport reactions (15)

[JHL-452b]

Current behavior:

50 genes are discovered to be uncorrelated with their corresponding reactions in Transport reactions.

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000102743	Q9Y619	MAR06314: H+ + histidine + ornithine --> H+ + histidine + ornithine	ENSG00000102743 or ENSG00000120329	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000102743 encodes a mitochondrial ornithine-citrulline antiporter which facilitates the exchange of ornithine and other amino acids like citrulline, arginine, and lysine along with protons (H+), critical for the urea cycle. However, the reaction provided involves H+ with histidine and ornithine, which is not mentioned among the catalytic activities or functions of the gene as specified in the annotations. Therefore, the gene is not related to the provided reaction.	No
ENSG00000125454	Q9HC21	MAR07827: dTDP + dUTP --> dTDP + dUTP	ENSG00000125454	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000125454 encodes a mitochondrial transporter that mediates the uptake of thiamine diphosphate into mitochondria, with its catalytic activity involving the exchange of thiamine diphosphate and thiamine phosphate. However, the reaction provided involves the conversion of dTDP and dUTP, which are different substrates unrelated to thiamine metabolism, indicating that the gene and the reaction are not related.	No
ENSG00000125454	Q9HC21	MAR07836: dTDP + dTTP --> dTDP + dTTP	ENSG00000125454	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000125454 encodes a mitochondrial transporter responsible for the uptake of thiamine diphosphate into mitochondria, and it exhibits a specific catalytic activity related to the exchange of thiamine diphosphate and thiamine phosphate. The reaction provided, "dTDP + dTTP --> dTDP + dTTP," does not involve these compounds or the described function of the gene, indicating no relevance between the two.	No
ENSG00000125454	Q9HC21	MAR07844: dADP + dCTP --> dADP + dCTP	ENSG00000125454	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000125454 encodes a mitochondrial transporter that mediates the uptake of thiamine diphosphate into mitochondria and facilitates an antiporter activity involving thiamine diphosphate and thiamine phosphate. The catalytic action described involves thiamine diphosphate and thiamine phosphate, while the reaction provided involves dADP and dCTP, which are unrelated to thiamine metabolism. Therefore, there is no connection between the gene's annotated function and the reaction given.	No
ENSG00000184343	Q9UPE1	MAR03008: ATP + H2O + propanoyl-CoA --> ADP + H+ + Pi + propanoyl-CoA	ENSG00000067829 or ENSG00000067840 or ENSG00000101986 or ENSG00000130821 or ENSG00000130822 or ENSG00000184343 or ENSG00000185825	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000184343 codes for a serine/arginine-rich protein-specific kinase, which phosphorylates serine and threonine residues in protein substrates. However, the reaction provided involves the conversion of ATP, H2O, and propanoyl-CoA to ADP, H+, Pi, and propanoyl-CoA, which is not related to the described kinase activity of phosphorylating protein serine/threonine residues. Therefore, the noted phosphorylation activity of the gene product does not align with the biochemical transformation described in the reaction.	No
ENSG00000130821	P48029	MAR03014: arachidonyl-CoA + ATP + H2O --> ADP + arachidonyl-CoA + H+ + Pi	ENSG00000067829 or ENSG00000067840 or ENSG00000101986 or ENSG00000130821 or ENSG00000130822 or ENSG00000184343 or ENSG00000185825	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000130821 encodes for a creatine:sodium symporter involved in the uptake of creatine and its transport across the blood-brain barrier. The catalytic activity of this gene involves the transport of chloride, creatine, and sodium ions. The reaction provided involves the conversion of arachidonyl-CoA, ATP, and water to ADP, arachidonyl-CoA, proton, and phosphate, which is unrelated to the function of the creatine:sodium symporter produced by this gene.	No
ENSG00000120253	Q8NFH3	MAR07796: UTP <=> UTP	(ENSG00000030066 and ENSG00000069248 and ENSG00000075188 and ENSG00000110713 and ENSG00000111581 and ENSG00000120253 and ENSG00000125450) or ENSG00000093000 or ENSG00000095319 or ENSG00000102900 or ENSG00000108559 or ENSG00000113569 or ENSG00000124789 or ENSG00000126883 or ENSG00000132182 or ENSG00000136243 or ENSG00000138750 or ENSG00000139496 or ENSG00000143552 or ENSG00000153201 or ENSG00000155561 or ENSG00000163002 or ENSG00000198088 or ENSG00000213024	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000120253 encodes a component of the Nup107-160 subcomplex of the nuclear pore complex (NPC), which is involved in NPC assembly, kinetochore microtubule attachment, mitotic progression, and chromosome segregation. It does not have any documented catalytic activity or any direct involvement in the reaction UTP <=> UTP.	No
ENSG00000205060	Q969S0	MAR04125: UDP-xylose + UMP <=> UDP-xylose + UMP	ENSG00000205060	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000205060 encodes an antiporter that transports nucleotide sugars across the endoplasmic reticulum membrane, specifically involved in exchanging UDP-alpha-D-glucuronate (UDP-GlcA) with UDP-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) or UDP-alpha-D-xylose (UDP-Xyl). The reactions catalyzed by this gene involve these specific nucleotide sugars. However, the reaction provided (UDP-xylose + UMP <=> UDP-xylose + UMP) does not involve the transport or exchange of nucleotide sugars across the ER membrane but rather a simple equilibrium reaction involving UDP-xylose and UMP, which is unrelated to the gene's described functions and catalytic activities.	No
ENSG00000205060	Q969S0	MAR08857: UDP-glucose + UMP <=> UDP-glucose + UMP	ENSG00000117620 or ENSG00000130958 or ENSG00000205060	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000205060 encodes an antiporter that transports nucleotide sugars across the endoplasmic reticulum (ER) membrane, specifically exchanging UDP-alpha-D-glucuronate and UDP-N-acetyl-alpha-D-glucosamine or UDP-alpha-D-xylose. In contrast, the reaction provided involves UDP-glucose and UMP, which are not substrates for the antiporter encoded by this gene. Therefore, the gene's function does not align with the reaction's substrates or mechanism.	No
ENSG00000163295	P09923	MAR08969: FMN + H2O --> Pi + riboflavin	ENSG00000163295	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000163295 encodes an enzyme responsible for alkaline phosphatase activity, which hydrolyzes phosphate monoesters to produce an alcohol and phosphate. The provided reaction, however, involves the hydrolysis of FMN (flavin mononucleotide) to produce phosphate (Pi) and riboflavin, which does not involve a phosphate monoester and does not align with the annotated function of the gene.	Not sure, keep it now.
ENSG00000023839	Q92887	MAR09882: ATP + folate + H2O --> ADP + folate + H+ + Pi	ENSG00000005471 or ENSG00000023839 or ENSG00000103222 or ENSG00000114770 or ENSG00000118777 or ENSG00000121270 or ENSG00000125257	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. According to the provided annotation for the gene ENSG00000023839, its function pertains to ATP-dependent transport of a wide variety of conjugated organic anions, drugs, and toxicants across cell membranes. It specifically mediates the excretion of mono- and bis-glucuronidated bilirubin, 17beta-estradiol glucuronide, leukotriene C4, and other substrates, but it does not mention any involvement with folate. Moreover, the reaction provided involves the catalysis of ATP and folate, which falls outside the documented activities of the gene. Therefore, the gene is not relevant to the ATP + folate reaction.	Not sure, keep it now.
ENSG00000073734	O95342	MAR11380: 5beta-cholestane-3alpha,7alpha,26-triol + ATP + H2O --> 5beta-cholestane-3alpha,7alpha,26-triol + ADP + H+ + Pi	ENSG00000073734 or ENSG00000108846 or ENSG00000121270	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000073734 encodes a protein that catalyzes the ATP-dependent transport of bile salts and other compounds across the canalicular membrane of hepatocytes, playing a role in bile acid and lipid homeostasis. The reaction provided (5beta-cholestane-3alpha,7alpha,26-triol + ATP + H2O --> 5beta-cholestane-3alpha,7alpha,26-triol + ADP + H+ + Pi) does not involve the transport of bile salts or related compounds, nor does it align with the known catalytic activities associated with the gene's protein function as described.	Yes
ENSG00000100170	P13866	MAR11410: galactose + 2.0 H+ <=> galactose + 2.0 H+	ENSG00000100170	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000100170 encodes for a Na(+)-coupled sugar symporter, which actively transports D-glucose and D-galactose along with Na(+) ions across the plasma membrane, driven by the Na(+) electrochemical gradient. The catalytic activities involve the transport of D-glucose or D-galactose coupled with Na(+), unlike the provided reaction, which involves a galactose and proton (H+) exchange. Thus, the gene's function does not align with the given reaction's description involving H+ ions.	No
ENSG00000165970	Q9Y345	MAR11456: chloride + isovalerylglycine + 3.0 Na+ <=> chloride + isovalerylglycine + 3.0 Na+	ENSG00000165970	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165970 codes for a sodium- and chloride-dependent glycine transporter involved in the reuptake of glycine for terminating neurotransmission at glycinergic synapses. The provided reaction involves the molecule isovalerylglycine and does not pertain to the reuptake or transport of glycine as outlined by the gene's function. Therefore, the gene's annotated function does not match the provided reaction.	No
ENSG00000173638	P41440	MAR11647: 20alpha-hydroxy-4-pregnen-3-one + hydroxide <=> 20alpha-hydroxy-4-pregnen-3-one + hydroxide	ENSG00000173638	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000173638 encodes an antiporter that imports reduced folates or a subset of cyclic dinucleotides and exports organic anions. The catalytic activities described for the antiporter include reactions involving folates and cyclic dinucleotides like 2',3'-cGAMP and 3',3'-cGAMP. However, the provided reaction involves "20alpha-hydroxy-4-pregnen-3-one" and hydroxide, which are not substrates of the antiporter encoded by this gene. Therefore, the gene and the reaction are not related.	No
ENSG00000142583	P22732	MAR11779: D-glucarate <=> D-glucarate	ENSG00000142583	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000142583 encodes a protein functioning as a fructose transporter with specific reference to its activity in transporting D-fructose across the cell membrane. The annotated catalytic activity explicitly states the reaction D-fructose(out) = D-fructose(in). However, the given reaction involving D-glucarate <=> D-glucarate does not involve fructose transport and falls outside the functional range of the gene. Therefore, the gene and the reaction are not related.	No
ENSG00000112759	Q99808	MAR11801: quinonoid dihydrobiopterin <=> quinonoid dihydrobiopterin	ENSG00000112759 or ENSG00000174669	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000112759 functions as a Na(+)-independent transporter involved in the transport of nucleosides and nucleobases, such as adenosine, guanosine, inosine, uridine, thymidine, and cytidine. This includes transporting purine and pyrimidine nucleobases across cellular membranes, maintaining cellular homeostasis, and regulating extracellular inosine levels. The reactions catalyzed by this gene involve the exchange of specific nucleosides and nucleobases in and out of cells. However, the reaction provided involves quinonoid dihydrobiopterin, which is unrelated to the transport activities of nucleosides and nucleobases facilitated by the gene. Therefore, the gene and the reaction are not related.	No
ENSG00000131686	P23280	MAR00190: ATP + H2O + lauric acid --> ADP + H+ + lauric acid + Pi	ENSG00000074410 or ENSG00000104267 or ENSG00000107159 or ENSG00000118298 or ENSG00000131686 or ENSG00000133742 or ENSG00000159593 or ENSG00000164879 or ENSG00000165029 or ENSG00000166747 or ENSG00000167434 or ENSG00000168748 or ENSG00000169239 or ENSG00000174990 or ENSG00000185015	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000131686 encodes for an enzyme that catalyzes the reversible hydration of carbon dioxide, as indicated in its function and catalytic activity. This enzyme's activity involves the conversion of H(+) and hydrogencarbonate to CO2 and H2O. In contrast, the reaction provided involves the conversion of ATP, H2O, and lauric acid to ADP, H+, lauric acid, and Pi. These two reactions involve entirely different substrates and products, indicating that the gene product does not participate in or influence the provided reaction.	No
ENSG00000165029	O95477	MAR00215: ATP + H2O + pentadecylic acid --> ADP + H+ + pentadecylic acid + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165029 encodes a protein that catalyzes the translocation of specific phospholipids, such as phosphatidylcholine and phosphatidylserine, as well as sphingomyelin and cholesterol across membranes, using ATP hydrolysis. However, the reaction provided involves ATP, H2O, and pentadecylic acid, which is not a substrate mentioned in the gene's known catalytic activities. Therefore, the gene's function does not align with the provided reaction.	No
ENSG00000165029	O95477	MAR00231: ATP + H2O + palmitolate --> ADP + H+ + palmitolate + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165029 encodes a protein that catalyzes the translocation of specific phospholipids such as phosphatidylcholine, phosphatidylserine, sphingomyelin, and cholesterol across membranes, coupled with the hydrolysis of ATP. The described reactions involve transferring these phospholipids and cholesterol between intracellular and extracellular spaces. However, the reaction provided (ATP + H2O + palmitolate --> ADP + H+ + palmitolate + Pi) involves palmitolate, which is not mentioned among the substrates handled by this gene product. Therefore, the gene does not match the specific reaction involving palmitolate.	No
ENSG00000165029	O95477	MAR00239: ATP + H2O + margaric acid --> ADP + H+ + margaric acid + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165029 encodes a protein that specifically catalyzes the translocation of phospholipids (such as phosphatidylcholine and phosphatidylserine) and cholesterol across the membrane utilizing ATP. However, the reaction provided involves the conversion of ATP and H2O with margaric acid (a type of fatty acid) to produce ADP, H+, margaric acid, and Pi. This reaction does not involve the translocation of phospholipids or cholesterol, which are the primary substrates for the gene product, indicating the gene is not relevant to the given reaction.	No
ENSG00000165029	O95477	MAR00269: ATP + elaidate + H2O --> ADP + elaidate + H+ + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000165029 is involved in the translocation of specific phospholipids, such as phosphatidylcholine, phosphatidylserine, sphingomyelin, and cholesterol, coupled with ATP hydrolysis. It plays a role in forming nascent high-density lipoproteins (HDLs) and phospholipid transfer to apolipoproteins. The reaction provided involves ATP, elaidate, and water to produce ADP, elaidate, H+, and phosphate, which does not correspond to the specific phospholipid translocation activity described for this gene. Therefore, the gene's function does not match the reaction.	No
ENSG00000165029	O95477	MAR00277: (6Z,9Z)-octadecadienoic acid + ATP + H2O --> (6Z,9Z)-octadecadienoic acid + ADP + H+ + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000165029 encodes a protein that catalyzes the translocation of specific phospholipids and cholesterol across membranes utilizing ATP, specifically involving phosphatidylcholine, phosphatidylserine, sphingomyelin, and cholesterol. The provided reaction involves (6Z,9Z)-octadecadienoic acid, which is a type of fatty acid and not a phospholipid, hence the gene's function does not encompass the given reaction.	No
ENSG00000165029	O95477	MAR00287: ATP + eicosanoate + H2O --> ADP + eicosanoate + H+ + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165029 encodes for a protein that is involved in the translocation of specific phospholipids and cholesterol from one leaflet of the membrane to the other, utilizing ATP hydrolysis. The gene products catalyze reactions involving phosphatidylcholine, phosphatidylserine, sphingomyelin, and cholesterol, but there is no evidence or annotation indicating its involvement in the translocation of eicosanoates. Therefore, the reaction involving ATP, eicosanoate, and H2O resulting in ADP, eicosanoate, H+, and Pi is not related to the gene's annotated functions.	No
ENSG00000165029	O95477	MAR00351: ATP + H2O + linolenate --> ADP + H+ + linolenate + Pi	ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165029 functions as a catalyst for the translocation of specific phospholipids, such as phosphatidylcholine, phosphatidylserine, sphingomyelin, and cholesterol, from the cytoplasmic to the extracellular/lumenal leaflet of membranes coupled to ATP hydrolysis. The provided reaction pertains to the conversion of ATP and linolenate to ADP, H+, linolenate, and Pi, which does not involve the translocation of phospholipids or cholesterol. Therefore, the gene's function is unrelated to the reaction given.	No
ENSG00000021488	P82251	MAR01902: HCO3- + serotonin + S-glutathionyl-2-4-dinitrobenzene <=> HCO3- + serotonin + S-glutathionyl-2-4-dinitrobenzene	ENSG00000021488 or ENSG00000084453 or ENSG00000111700	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000021488 functions as a transporter complex that mediates the exchange of cationic and neutral amino acids. It specifically catalyzes reactions involving various amino acids like L-arginine, L-leucine, L-histidine, L-phenylalanine, L-cysteine, L-cystine, and L-lysine. However, the provided reaction involves HCO3-, serotonin, and S-glutathionyl-2-4-dinitrobenzene, which are not substrates or products in any of the reactions catalyzed by the transporter associated with this gene. Therefore, there is no direct relevance between the gene and the provided reaction.	No
ENSG00000021488	P82251	MAR01904: acetylcholine + glutathionyl-leukotriene C4 + HCO3- <=> acetylcholine + glutathionyl-leukotriene C4 + HCO3-	ENSG00000021488 or ENSG00000084453 or ENSG00000111700	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000021488 encodes a protein that forms a transporter complex responsible for the electrogenic exchange of cationic and neutral amino acids, such as L-arginine, L-leucine, L-histidine, L-phenylalanine, L-cysteine, and L-cystine. However, the reaction involves molecules like acetylcholine, glutathionyl-leukotriene C4, and bicarbonate ion (HCO3-), which are unrelated to the amino acid transport facilitated by the gene's protein product. Therefore, there is no functional link between the gene and the given reaction.	No
ENSG00000108932	O15403	MAR04935: acetate + H+ --> acetate + H+	ENSG00000100156 or ENSG00000108932 or ENSG00000118596 or ENSG00000141526 or ENSG00000155380 or ENSG00000168679 or ENSG00000170190	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000108932 encodes for a monocarboxylate transporter that is selective for taurine, facilitating its transport across the plasma membrane. The annotated reaction involves taurine transport, while the provided reaction describes the conversion of acetate and H+, which does not involve taurine and falls outside the gene's functional scope.	No
ENSG00000141526	O15427	MAR04935: acetate + H+ --> acetate + H+	ENSG00000100156 or ENSG00000108932 or ENSG00000118596 or ENSG00000141526 or ENSG00000155380 or ENSG00000168679 or ENSG00000170190	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000141526 encodes a proton-dependent transporter of monocarboxylates, facilitating the transport of L-lactate and pyruvate. The described reactions involve transporting (S)-lactate and pyruvate across the membrane. However, the provided reaction involves acetate and H+, which is not the same as the substrates L-lactate or pyruvate. Thus, the gene's function does not align with the provided acetate reaction.	Yes, acetate belongs to monocarboxylates which can be transported by this transporter.
ENSG00000163581	P11168	MAR04973: mannose <=> mannose	ENSG00000059804 or ENSG00000117394 or ENSG00000163581	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000163581 encodes a facilitative hexose transporter that mediates the transport of glucose, fructose, galactose, and dehydroascorbate, but it is not involved in the transport or catalysis of mannose, as indicated in the reaction provided. Therefore, this gene is not relevant to the mannose reaction.	Yes, mannose belongs to hexose which can be transported by this transporter.
ENSG00000100156	O95907	MAR05018: (R)-3-hydroxybutanoate + H+ --> (R)-3-hydroxybutanoate + H+	ENSG00000100156 or ENSG00000108932 or ENSG00000118596 or ENSG00000141526 or ENSG00000155380 or ENSG00000168679 or ENSG00000170190	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000100156 is annotated to be involved in the transport of (S)-lactate and H+ across the retinal pigment epithelium, playing a role in pH and ion homeostasis of the outer retina. The reaction provided, however, involves (R)-3-hydroxybutanoate and H+, which is different from (S)-lactate. Therefore, the gene's function does not relate to the reaction specified.	No
ENSG00000167703	Q8N370	MAR05070: histidine <=> histidine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000167703 encodes a uniporter that specifically mediates the transport of L-phenylalanine, L-methionine, and L-branched-chain amino acids such as L-leucine and L-isoleucine, using facilitated diffusion. However, the provided reaction involves the transport of histidine, which is not one of the amino acids mentioned in the gene's described function or catalytic activities. Therefore, based on the annotation, the gene and the reaction are not related.	No
ENSG00000139514	P30825	MAR05070: histidine <=> histidine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000139514 encodes a high-affinity, low-capacity permease involved in the transport of the cationic amino acids arginine, lysine, and ornithine in non-hepatic tissues. It facilitates the inward and outward transport of L-arginine, L-lysine, L-ornithine, and L-homoarginine. However, it does not facilitate the transport of histidine, which is the reaction mentioned. Therefore, the gene is not relevant to the histidine transport reaction.	No
ENSG00000165349	Q8WY07	MAR05074: tyrosine <=> tyrosine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000112394 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: ENSG00000165349 gene mediates the uptake of cationic L-amino acids such as L-arginine, L-lysine, and L-ornithine but does not catalyze the conversion or transportation of tyrosine. The reactions associated with the gene specifically involve L-arginine, L-lysine, and L-ornithine, as evidenced by the cited references. Therefore, it does not have any functional or catalytic role related to the reaction involving tyrosine.	No
ENSG00000139514	P30825	MAR05074: tyrosine <=> tyrosine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000112394 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000139514 encodes a high-affinity, low-capacity permease involved in the transport of specific cationic amino acids, including arginine, lysine, ornithine, and homoarginine, as evidenced by various PubMed sources. The annotated catalytic activities of this gene product do not include any reaction involving tyrosine. Therefore, the gene and the provided tyrosine reaction are not related.	No
ENSG00000165349	Q8WY07	MAR05076: glycine --> glycine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000130876 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000164363 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000165349 encodes a protein that functions as a uniporter responsible for the uptake of cationic L-amino acids such as L-arginine, L-lysine, and L-ornithine, through a sodium ions- and pH-independent mechanism involving passive diffusion. It facilitates the translocation of these specific amino acids across the membrane, as evidenced by the cited study. However, the reaction provided involves only glycine, which is not one of the substrates mentioned in the gene's functional or catalytic activity description. Therefore, the gene's function does not align with the given reaction involving glycine.	No
ENSG00000165349	Q8WY07	MAR05078: glutamine <=> glutamine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000165349 encodes for a uniporter that specifically mediates the uptake of cationic L-amino acids such as L-arginine, L-lysine, and L-ornithine. The catalytic activities annotated for this gene involve the transport of these specific amino acids and are not related to the reaction involving glutamine. Therefore, based on the annotation of the gene, it does not participate in the reaction converting glutamine.	No
ENSG00000003989	P52569	MAR05078: glutamine <=> glutamine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000003989 is a permease involved in the transport of cationic amino acids such as L-arginine, L-lysine, L-ornithine, and L-homoarginine. The catalytic activities described for the gene involve the transport of these specific amino acids across cellular membranes. However, the reaction provided is related to the transport of glutamine, which is not mentioned as a substrate for the permease function of this gene. Therefore, the gene does not participate in the glutamine transport reaction.	No
ENSG00000149150	O75387	MAR05080: tryptophan <=> tryptophan	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000112394 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000149150 mediates the transport of specific neutral amino acids such as L-leucine, L-isoleucine, L-valine, and L-phenylalanine, as indicated by its function and multiple catalytic activities. The annotated catalytic activities do not include the transport or reaction involving tryptophan. Therefore, the gene does not relate to the reaction involving tryptophan.	Yes, tryptophan is a neutral amino acid.
ENSG00000165349	Q8WY07	MAR05088: asparagine --> asparagine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165349 is involved in mediating the uptake of cationic L-amino acids such as L-arginine, L-lysine, and L-ornithine through passive diffusion, as evidenced by catalytic activities documented in various publications. However, the provided reaction involves asparagine, which is not a cationic L-amino acid and falls outside the transport activities mediated by the gene ENSG00000165349. Therefore, the gene does not influence the asparagine reaction.	No
ENSG00000149150	O75387	MAR05088: asparagine --> asparagine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000149150 encodes a protein that transports neutral amino acids such as L-leucine, L-isoleucine, L-valine, and L-phenylalanine. Its transport activity is sodium ions-independent and electroneutral, mediated by facilitated diffusion. The catalytic activities listed involve the transport of L-leucine, L-isoleucine, L-methionine, L-phenylalanine, and L-valine. However, the reaction provided involves the conversion of asparagine to asparagine, which is unrelated to the function and catalytic activities of the gene ENSG00000149150. Therefore, the gene is not relevant to the reaction provided.	Yes, asparagine is a neutral amino acid.
ENSG00000149150	O75387	MAR05091: threonine --> threonine	(ENSG00000092068 and ENSG00000168003) or ENSG00000003989 or ENSG00000103257 or ENSG00000123643 or ENSG00000130876 or ENSG00000139514 or ENSG00000149150 or ENSG00000155465 or ENSG00000165349 or ENSG00000167703	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000149150 encodes a transporter protein that facilitates the transport of neutral amino acids such as L-leucine, L-isoleucine, L-valine, L-phenylalanine, and L-methionine, among others, across cell membranes. The specific reactions mentioned in the annotation involve the transport of these amino acids. However, the provided reaction involves threonine, which is not listed among the substrates transported by the protein encoded by the gene ENSG00000149150. Therefore, the gene is not relevant to the reaction involving threonine.	Yes
ENSG00000139209	Q969I6	MAR05315: leucine + Na+ --> leucine + Na+	ENSG00000003989 or ENSG00000072041 or ENSG00000111371 or ENSG00000134294 or ENSG00000139209 or ENSG00000139514 or ENSG00000165349 or ENSG00000168003 or ENSG00000174358	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000139209 encodes a symporter involved in the transport of neutral amino acids and sodium ions across the cell membrane. It specifically transports smaller amino acids such as glycine, L-alanine, L-serine, L-asparagine, L-threonine, L-cysteine, L-histidine, L-proline, L-glutamine, and L-methionine. The reaction provided involves leucine and Na+, which is not listed among the substrates that the symporter encoded by the gene ENSG00000139209 transports. Therefore, the gene is not relevant to the reaction involving leucine and Na+.	Yes
ENSG00000111371	Q9H2H9	MAR05319: Na+ + phenylalanine --> Na+ + phenylalanine	ENSG00000003989 or ENSG00000111371 or ENSG00000134294 or ENSG00000139209 or ENSG00000139514 or ENSG00000165349 or ENSG00000174358	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000111371 encodes a symporter that cotransports short-chain neutral amino acids and sodium ions across the cell membrane. It shows catalytic activity in the transport of amino acids like L-glutamine, L-alanine, L-asparagine, L-histidine, L-serine, L-cysteine, L-methionine, glycine, L-threonine, and L-proline in the presence of Na+. However, the reaction Na+ + phenylalanine --> Na+ + phenylalanine does not correspond to the catalytic activities described for this gene because phenylalanine is not listed among the amino acids transported by this symporter.	No
ENSG00000154025	A0PJK1	MAR05450: glucose + Na+ --> glucose + Na+	ENSG00000117834 or ENSG00000140675 or ENSG00000148942 or ENSG00000154025 or ENSG00000158865	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000154025 encodes for a Na+-coupled sugar symporter that specifically transports D-mannose and D-fructose, with no mention of glucose transport activity. The specified reactions involve D-mannose and D-fructopyranose, whereas the provided reaction involves glucose, indicating that the transport activity of the gene product does not relate to the transport of glucose and Na+.	Not sure, keep it now.
ENSG00000103257	Q01650	MAR05467: glycine + proline <=> glycine + proline	ENSG00000103257	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000103257 encodes for a protein that functions primarily as a high-affinity transporter mediating the uptake and exchange of large neutral amino acids, thyroid hormones, and other selective substances across membranes. It does not have any known catalytic activity related to the reaction involving glycine and proline. The described functions and catalytic activities of the gene do not include any involvement with glycine and proline, thereby making it not relevant to the given reaction.	No
ENSG00000165029	O95477	MAR05540: homoserine + phenylalanine <=> homoserine + phenylalanine	ENSG00000103257 or ENSG00000165029	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165029 encodes a protein that functions in the translocation of specific phospholipids and cholesterol across cellular membranes using ATP hydrolysis. Its annotated catalytic activities involve reactions with phospholipids (such as phosphatidylcholine, phosphatidylserine, and sphingomyelin) and cholesterol. However, the reaction provided is a simple exchange reaction involving homoserine and phenylalanine, which is unrelated to the lipid translocation or ATP-dependent activities carried out by the gene's protein product. Therefore, the gene does not participate in the given reaction.	No
ENSG00000103257	Q01650	MAR05575: homoserine + threonine <=> homoserine + threonine	ENSG00000103257	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000103257 encodes a protein that functions as a sodium-independent, high-affinity transporter mediating the uptake and exchange of large neutral amino acids, thyroid hormones, and certain toxins across cell membranes. The specific reactions facilitated by this gene involve amino acids such as phenylalanine, tryptophan, histidine, leucine, isoleucine, valine, tyrosine, methionine, and alanine, as well as thyroid hormones. However, the provided reaction involves homoserine and threonine, which are not substrates associated with the transport activities of the protein encoded by this gene. Therefore, the gene is not relevant to the reaction involving homoserine and threonine.	No
ENSG00000103257	Q01650	MAR05588: glutamine + glycine + Na+ --> glutamine + glycine + Na+	ENSG00000103064 or ENSG00000103257	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000103257 encodes a protein that functions primarily as a transporter of large neutral amino acids and thyroid hormones across cell membranes in a sodium-independent manner. The reaction provided involves the transfer of glutamine and glycine along with sodium ions, which falls under the category of sodium-dependent transport processes. Therefore, based on its annotated function, the gene ENSG00000103257 does not relate to the reaction described.	No
ENSG00000103064	Q92536	MAR05606: alanine + Na+ + tryptophan --> alanine + Na+ + tryptophan	ENSG00000103064 or ENSG00000103257	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000103064 encodes a protein functioning as an antiporter primarily involved in the exchange of cationic and neutral amino acids such as L-arginine, L-lysine, L-leucine, L-glutamine, and others, often coupled with sodium ions. The given reaction (alanine + Na+ + tryptophan --> alanine + Na+ + tryptophan) does not involve these amino acids and does not describe an antiport exchange mechanism, indicating the gene's products do not participate in this reaction.	No

[hhl-24]

keep gene ENSG00000163295 for MAR08969: FMN + H2O --> Pi + riboflavin, encoded protein P09923 has catalytic activity(a phosphate monoester + H2O = an alcohol + phosphate).

for MAR11410, the gene ENSG00000100170 encodes a transporter that facilitates the movement of galactose using sodium ions (Na+) instead of hydrogen ions (H+), thereby it seems to modify the reaction.

[JHL-452b]

Aha, It looks like we got some same opinions about ENSG00000163295 for MAR08969 and ENSG00000100170 for MAR11410, which both checked in last column of the table. Do you have any other disagreements?

[hhl-24]

Others seem fine to me.