GPR curation for Glycosphingolipid metabolism and Glycosylphosphatidylinositol (GPI)-anchor biosynthesis (1)

[JHL-452b]

Current behavior:

26 genes are discovered to be uncorrelated with their corresponding reactions in Glycosphingolipid metabolism and Glycosylphosphatidylinositol (GPI)-anchor biosynthesis.

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000174684	O43505	MAR00889: paragloboside + UDP-N-acetylglucosamine --> H+ + nLc5Cer + UDP	ENSG00000170340 or ENSG00000174684 or ENSG00000176383 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000174684 encodes for a beta-1,4-glucuronyltransferase involved in the O-mannosylation of alpha-dystroglycan (DAG1), where it transfers a glucuronic acid residue onto a xylose acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer. This function is related to the synthesis of phosphorylated O-mannosyl glycan, necessary for binding extracellular proteins with high affinity. In contrast, the provided reaction involves the conversion of paragloboside to nLc5Cer, which is unrelated to the function of the gene, as it involves different substrates and products. The gene's activity is specific to the O-mannosylation process, not the synthesis pathway described in the provided reaction.	No
ENSG00000102595	Q9NYU1	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000102595 encodes for a protein involved in the quality control of protein folding in the endoplasmic reticulum by reglucosylating misfolded glycoproteins. Its catalytic activity involves the reglucosylation of N-glycans on misfolded proteins. However, the provided reaction describes the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide, H+, and UDP, which does not involve N-glycan reglucosylation nor is it related to the protein folding quality control process. Therefore, the gene's function and catalytic activity do not relate to the provided reaction.	No
ENSG00000134910	P46977	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The function and catalytic activity of the gene ENSG00000134910 are associated with the transfer of a glycan to an asparagine residue in nascent polypeptide chains during N-glycosylation. This process involves the oligosaccharyl transferase (OST) complex and does not relate to the reaction described, which involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide, H+, and UDP. The specific reaction described entails sphingolipid metabolism, not protein N-glycosylation.	No
ENSG00000163527	Q8TCJ2	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000163527 encodes the catalytic subunit of the oligosaccharyl transferase (OST) complex, which is involved in the process of N-glycosylation of nascent polypeptides in the endoplasmic reticulum. Its function is to transfer a glycan from dolichol-pyrophosphate to an asparagine residue within a specific consensus motif. In contrast, the provided reaction describes the transfer of a galactose from UDP-galactose to D-galactosyl-N-acylsphingosine to form digalactosylceramide. These reactions involve different substrates, products, enzymes, and biological processes, thus making the gene not relevant to the provided reaction.	No
ENSG00000119227	Q86VD9	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000119227 codes for a mannosyltransferase involved in glycosylphosphatidylinositol (GPI) anchor biosynthesis, specifically transferring a fourth mannose to trimannosyl-GPIs. The reaction provided involves the transformation of D-galactosyl-N-acylsphingosine and UDP-galactose into digalactosylceramide, H+, and UDP, which does not involve GPI anchor biosynthesis or mannosyltransferase activity. Thus, the gene and the reaction are not related.	No
ENSG00000177191	Q7Z7M8	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000177191 encodes an enzyme involved in the elongation of specific branch structures of multiantennary N-glycans and shows strong activity towards tetraantennary N-glycans and 2,6 triantennary glycans. The reaction described involves the synthesis of digalactosylceramide from D-galactosyl-N-acylsphingosine and UDP-galactose, which is not related to the function of the enzyme encoded by the gene.	No
ENSG00000069943	Q92521	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000069943 encodes a mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis, transferring the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly. The provided reaction involves D-galactosyl-N-acylsphingosine and UDP-galactose to form digalactosylceramide, H+, and UDP, which is unrelated to the function of the gene mentioned.	No
ENSG00000175711	Q67FW5	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000175711 codes for a glycosyltransferase that galactosylates queuosine(34) in tRNA(Tyr), using UDP-alpha-D-galactose. The provided reaction involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose into digalactosylceramide, H+, and UDP, which is unrelated to the specific tRNA modification described for the gene's product.	No
ENSG00000139044	Q6L9W6	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000139044 encodes an enzyme that transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl, forming N,N'-diacetyllactosediamine with a beta-1,4-linkage in N-linked and possibly O-linked glycans. However, the reaction provided involves D-galactosyl-N-acylsphingosine and UDP-galactose to form digalactosylceramide, which is not the same substrate or product involved in the gene's annotated catalytic activity. Therefore, the gene and the reaction are not related.	No
ENSG00000156966	Q8NFL0	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000156966 encodes for an enzyme that transfers N-acetyl glucosamine (GlcNAc) via a beta1->3 linkage from UDP-GlcNAc to the non-reducing terminal galactose (Gal) in the synthesis of keratan sulfate proteoglycans, primarily affecting corneal transparency and tissue architecture. In contrast, the reaction provided involves the transfer of galactose from UDP-galactose to D-galactosyl-N-acylsphingosine to form digalactosylceramide, which is a different biochemical process unrelated to the function of the gene product described.	No
ENSG00000060642	Q9NUD9	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000060642 encodes an enzyme that functions as an alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. This means it transfers a mannose to the glycosylphosphatidylinositol (GPI) during GPI precursor assembly. However, the provided reaction involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide, H+, and UDP, which involves galactosyltransferase activity, not mannosyltransferase activity. Therefore, the function of the gene does not align with the reaction described.	No
ENSG00000162885	Q8NCR0	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000162885 encodes a Beta-1,3-N-acetylgalactosaminyltransferase that participates in the synthesis of a specific carbohydrate structure on N- and O-glycans and is involved in alpha-dystroglycan glycosylation. Its catalytic activity involves the formation of a trisaccharide structure on protein O-mannose. On the other hand, the provided reaction describes the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide. The two processes involve different enzymes, substrates, and products, confirming that the gene and the reaction are unrelated.	No
ENSG00000172461	Q9Y231	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000172461 encodes a protein that catalyzes the transfer of fucose from GDP-beta-L-fucose to N-acetyl glucosamine within glycan structures. In contrast, the reaction provided involves the transfer of galactose from UDP-galactose to D-galactosyl-N-acylsphingosine to form digalactosylceramide, which does not involve fucose or the specific substrates and products catalyzed by the gene's protein. Therefore, the gene is not relevant to the reaction provided.	No
ENSG00000176383	Q9C0J1	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000176383 codes for a Beta-1,3-N-acetylglucosaminyltransferase that specifically functions in synthesizing poly-N-acetyllactosamine through the addition of N-acetyl-beta-D-glucosaminyl residues to beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivatives. This is evidenced by its catalytic activity which involves the reaction between a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative and UDP-N-acetyl-alpha-D-glucosamine. However, the reaction provided¡ªD-galactosyl-N-acylsphingosine + UDP-galactose forming digalactosylceramide¡ªis entirely different in terms of substrates, products, and type of glycosylation, indicating no involvement of the Beta-1,3-N-acetylglucosaminyltransferase encoded by ENSG00000176383.	No
ENSG00000147162	O15294	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000147162 encodes a protein that functions as an O-GlcNAc transferase, catalyzing the transfer of an N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues of proteins, which is different from the reaction involving the transfer of galactose to ceramide to form digalactosylceramide. Thus, the gene's function does not match the provided reaction.	No
ENSG00000136731	Q9NYU2	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000136731 encodes a protein that recognizes glycoproteins with minor folding defects and reglucosylates single N-glycans near the misfolded part of the protein, functioning primarily in protein quality control within the endoplasmic reticulum. The catalytic activity described for the gene's product involves the reglucosylation of specific N-glycans. In contrast, the reaction provided involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide, H+, and UDP, which is unrelated to the function and catalytic activity of the gene's product. Therefore, the gene and the reaction are not related.	No
ENSG00000180549	Q11130	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000180549 encodes for an enzyme that specifically catalyzes the transfer of L-fucose from GDP-beta-L-fucose to various N-acetyl glucosamine-containing substrates, primarily in the synthesis of sialyl Lewis X structures and related compounds. However, the provided reaction involves the transfer of D-galactose from UDP-galactose to D-galactosyl-N-acylsphingosine, producing digalactosylceramide. This indicates a different enzymatic activity and substrate specificity, unrelated to the fucosyltransferase function of the gene product in question. Therefore, the gene is not relevant to the reaction provided.	No
ENSG00000085998	Q8WZA1	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000085998 is involved in O-mannosyl glycosylation, specifically catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Its catalytic activity involves the synthesis of GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr. In contrast, the provided reaction involves D-galactosyl-N-acylsphingosine and UDP-galactose, resulting in the formation of digalactosylceramide and H+. These reactions occur in different biochemical pathways, and the enzyme encoded by ENSG00000085998 does not act on the substrates or products involved in the given reaction.	No
ENSG00000143315	Q9H3S5	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000143315 encodes a mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis, which transfers mannose to GlcN-acyl-PI. In contrast, the reaction described involves D-galactosyl-N-acylsphingosine and UDP-galactose, and results in the production of digalactosylceramide, H+, and UDP. The functions and substrates involved in the gene¡¯s activity and the reaction do not match.	No
ENSG00000198488	Q6ZMB0	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000198488 encodes an enzyme, Beta-1,3-N-acetylglucosaminyltransferase, which is involved in the synthesis of mucin-type O-glycans. Its primary function is to catalyze the addition of N-acetylglucosamine to existing glycan structures on proteins. The provided reactions involve this enzyme's activity with substrates such as UDP-N-acetyl-alpha-D-glucosamine and protein-linked N-acetyl-alpha-D-galactosaminyl. However, the unrelated reaction provided (D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP) pertains to a different enzymatic activity involving sphingolipids and does not align with the specific function and substrates of the gene ENSG00000198488.	No
ENSG00000167889	Q3V5L5	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000167889 encodes a glycosyltransferase that specifically catalyzes the transfer of N-acetylglucosamine (GlcNAc) to mannose residues in N-glycans and O-mannosyl glycans. It does not participate in reactions involving the transfer of galactose. The provided reaction involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide and H+, which is not related to the activities of the glycosyltransferase encoded by ENSG00000167889. Therefore, the gene and the reaction are not related.	Not True
ENSG00000134910	P46977	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000134910 is involved in the N-glycosylation process, catalyzing the transfer of a glycan from dolichol-pyrophosphate to an asparagine residue in nascent polypeptide chains. Its catalytic activity specifically involves the formation of N(4)-(oligosaccharide-(1->4)-N-acetyl-beta-D-glucosaminyl) asparagine-linked peptides. The given reaction, however, involves the synthesis of digalactosylceramide from D-galactosyl-N-acylsphingosine and UDP-galactose, which is not related to the protein N-glycosylation pathway or the substrate specificity of the gene's encoded enzyme. Therefore, the gene and the reaction are not related.	No
ENSG00000069943	Q92521	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000069943 encodes a mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis, specifically transferring the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly. The reaction described involves D-galactosyl-N-acylsphingosine and UDP-galactose to form digalactosylceramide, which is unrelated to the function of the gene product.	No
ENSG00000162885	Q8NCR0	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000162885 encodes a protein that functions as a Beta-1,3-N-acetylgalactosaminyltransferase, which is involved in synthesizing the carbohydrate structure GalNAc-beta-1-3GlcNAc on N- and O-glycans and is specifically involved in the glycosylation of alpha-dystroglycan. Its catalytic activity includes the transfer of N-acetylgalactosamine onto a glycoprotein substrate using UDP-N-acetyl-alpha-D-galactosamine as a donor. However, the provided reaction involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose into digalactosylceramide and UDP, which does not involve N-acetylgalactosamine transferase activity and pertains to a different substrate and product. Thus, the specific function and catalytic activity of the gene do not match the provided reaction.	No
ENSG00000176383	Q9C0J1	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000176383 encodes for a Beta-1,3-N-acetylglucosaminyltransferase, which is involved in the synthesis of poly-N-acetyllactosamine and has activity for type 2 oligosaccharides. The catalytic activity described involves transferring N-acetylglucosamine to a specific substrate. In contrast, the reaction provided involves the synthesis of digalactosylceramide from D-galactosyl-N-acylsphingosine and UDP-galactose, which does not align with the gene's annotated function, substrate specificity, or catalytic activity.	No
ENSG00000136731	Q9NYU2	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000136731 is involved in the reglucosylation of glycoproteins with minor folding defects, aiding in protein folding quality control in the endoplasmic reticulum. The catalytic activity described involves the addition of a glucose residue to N-glycans on misfolded proteins. In contrast, the reaction provided converts D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide, H+, and UDP, which involves the synthesis of digalactosylceramide and is unrelated to the gene's function and catalytic activity associated with protein folding and reglucosylation.	No

[JHL-452b]

My knowledge about glycosphingolipid metabolism is very limited, so I really hope that someone with relevant background can help review it. 👀🌹

[hhl-24]

ALL of these seem fine to me.

[JHL-452b]

Could you please help us to review this issue? @feiranl

[feiranl]

@edkerk Ed, Could you also help review this issue due to your expertise in lipid metabolism, Thanks!

[feiranl]

As far as I can see I would say the GPT review and manual check are correct.

[edkerk]

I'll have a final check as well, tbd next week

[edkerk]

LGTM!