diff --git a/Tutorials-space/t_slat/index.html b/Tutorials-space/t_slat/index.html
index d224ef3..9913fa0 100644
--- a/Tutorials-space/t_slat/index.html
+++ b/Tutorials-space/t_slat/index.html
@@ -2266,7 +2266,7 @@ <h2 id="visualization-of-alignment">Visualization of alignment<a class="anchor-l
            <span class="n">width</span><span class="o">=</span><span class="mi">1200</span><span class="p">,</span>
            <span class="n">height</span><span class="o">=</span><span class="mi">1000</span><span class="p">,</span>
            <span class="n">return_fig</span><span class="o">=</span><span class="kc">True</span><span class="p">)</span>
-<span class="c1">#fig.show()</span>
+<span class="n">fig</span><span class="o">.</span><span class="n">show</span><span class="p">()</span>
 </pre></div>
 <div class="clipboard-copy-txt" id="cell-14">fig=Sankey_multi(adata_li=[adata1,adata2],
              prefix_li=['E11.5','E12.5'],
@@ -2282,7 +2282,7 @@ <h2 id="visualization-of-alignment">Visualization of alignment<a class="anchor-l
            width=1200,
            height=1000,
            return_fig=True)
-#fig.show()</div>
+fig.show()</div>
 </div>
 </div>
 </div>
@@ -2324,6 +2324,33 @@ <h2 id="visualization-of-alignment">Visualization of alignment<a class="anchor-l
 </div>
 </div>
 </div>
+</div><div class="jp-Cell jp-CodeCell jp-Notebook-cell jp-mod-noOutputs">
+<div class="jp-Cell jp-CodeCell jp-Notebook-cell jp-mod-noOutputs">
+<div class="jp-Cell-inputWrapper">
+<div class="jp-Collapser jp-InputCollapser jp-Cell-inputCollapser">
+</div>
+<div class="jp-InputArea jp-Cell-inputArea">
+<div class="jp-InputPrompt jp-InputArea-prompt">In [34]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
+<div class="CodeMirror cm-s-jupyter">
+<div class="zeroclipboard-container">
+<clipboard-copy for="cell-15">
+<div>
+<span class="notice" hidden="">Copied!</span>
+<svg aria-hidden="true" class="clipboard-copy-icon" data-view-component="true" height="20" version="1.1" viewbox="0 0 16 16" width="20">
+<path d="M0 6.75C0 5.784.784 5 1.75 5h1.5a.75.75 0 010 1.5h-1.5a.25.25 0 00-.25.25v7.5c0 .138.112.25.25.25h7.5a.25.25 0 00.25-.25v-1.5a.75.75 0 011.5 0v1.5A1.75 1.75 0 019.25 16h-7.5A1.75 1.75 0 010 14.25v-7.5z" fill="currentColor" fill-rule="evenodd"></path>
+<path d="M5 1.75C5 .784 5.784 0 6.75 0h7.5C15.216 0 16 .784 16 1.75v7.5A1.75 1.75 0 0114.25 11h-7.5A1.75 1.75 0 015 9.25v-7.5zm1.75-.25a.25.25 0 00-.25.25v7.5c0 .138.112.25.25.25h7.5a.25.25 0 00.25-.25v-7.5a.25.25 0 00-.25-.25h-7.5z" fill="currentColor" fill-rule="evenodd"></path>
+</svg>
+</div>
+</clipboard-copy>
+</div>
+<div class="highlight-ipynb hl-python"><pre><span></span><span class="n">fig</span><span class="o">.</span><span class="n">write_html</span><span class="p">(</span><span class="s2">"slat_sankey.html"</span><span class="p">)</span>
+</pre></div>
+<div class="clipboard-copy-txt" id="cell-15">fig.write_html("slat_sankey.html")</div>
+</div>
+</div>
+</div>
+</div>
+</div>
 </div>
 <div class="jp-Cell jp-MarkdownCell jp-Notebook-cell">
 <div class="jp-Cell-inputWrapper">
@@ -2345,7 +2372,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [27]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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@@ -2374,7 +2401,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                          <span class="p">}</span>
                         <span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-15">color_dict1=dict(zip(adata1.obs['annotation'].cat.categories,
+<div class="clipboard-copy-txt" id="cell-16">color_dict1=dict(zip(adata1.obs['annotation'].cat.categories,
                     adata1.uns['annotation_colors'].tolist()))
 adata1_df = pd.DataFrame({'index':range(embd0.shape[0]),
                           'x': adata1.obsm['spatial'][:,0],
@@ -2406,7 +2433,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [28]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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@@ -2421,7 +2448,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                                  <span class="n">subsample_size</span><span class="o">=</span><span class="mi">10000</span><span class="p">,</span> <span class="n">highlight_line</span> <span class="o">=</span> <span class="p">[</span><span class="s1">'blue'</span><span class="p">],</span> <span class="n">scale_coordinate</span> <span class="o">=</span> <span class="kc">True</span> <span class="p">)</span>
 <span class="n">kidney_align</span><span class="o">.</span><span class="n">draw_3D</span><span class="p">(</span><span class="n">size</span><span class="o">=</span> <span class="p">[</span><span class="mi">6</span><span class="p">,</span> <span class="mi">6</span><span class="p">],</span> <span class="n">line_width</span> <span class="o">=</span><span class="mf">0.8</span><span class="p">,</span> <span class="n">point_size</span><span class="o">=</span><span class="p">[</span><span class="mf">0.6</span><span class="p">,</span><span class="mf">0.6</span><span class="p">],</span> <span class="n">hide_axis</span><span class="o">=</span><span class="kc">True</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-16">kidney_align = match_3D_celltype(adata1_df, adata2_df, matching, meta='celltype', 
+<div class="clipboard-copy-txt" id="cell-17">kidney_align = match_3D_celltype(adata1_df, adata2_df, matching, meta='celltype', 
                                  highlight_celltype = [['Urogenital ridge'],['Kidney','Ovary']],
                                  subsample_size=10000, highlight_line = ['blue'], scale_coordinate = True )
 kidney_align.draw_3D(size= [6, 6], line_width =0.8, point_size=[0.6,0.6], hide_axis=True)</div>
@@ -2472,7 +2499,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [15]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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@@ -2498,7 +2525,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
     <span class="c1">#query_obs=adata2_df.loc[adata2_df['celltype']==query_cell,'target_obs_names'].tolist()</span>
     <span class="k">return</span> <span class="n">query_adata</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-17">def cal_matching_cell(target_adata,query_adata,matching,query_cell,clusters='annotation',):
+<div class="clipboard-copy-txt" id="cell-18">def cal_matching_cell(target_adata,query_adata,matching,query_cell,clusters='annotation',):
     adata1_df = pd.DataFrame({'index':range(target_adata.shape[0]),
                           'x': target_adata.obsm['spatial'][:,0],
                           'y': target_adata.obsm['spatial'][:,1],
@@ -2540,7 +2567,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [21]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
 <div class="zeroclipboard-container">
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@@ -2556,7 +2583,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                               <span class="n">query_cell</span><span class="o">=</span><span class="s1">'Kidney'</span><span class="p">,</span><span class="n">clusters</span><span class="o">=</span><span class="s1">'annotation'</span><span class="p">)</span>
 <span class="n">query_adata</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-18">query_adata=cal_matching_cell(target_adata=adata1,
+<div class="clipboard-copy-txt" id="cell-19">query_adata=cal_matching_cell(target_adata=adata1,
                               query_adata=adata2,
                               matching=matching,
                               query_cell='Kidney',clusters='annotation')
@@ -2592,7 +2619,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [17]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
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@@ -2605,7 +2632,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="highlight-ipynb hl-python"><pre><span></span><span class="n">adata1</span><span class="o">.</span><span class="n">obs</span><span class="p">[</span><span class="s1">'kidney_anno'</span><span class="p">]</span><span class="o">=</span><span class="s1">''</span>
 <span class="n">adata1</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">loc</span><span class="p">[</span><span class="n">query_adata</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">index</span><span class="p">,</span><span class="s1">'kidney_anno'</span><span class="p">]</span><span class="o">=</span><span class="n">query_adata</span><span class="o">.</span><span class="n">obs</span><span class="p">[</span><span class="s1">'annotation'</span><span class="p">]</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-19">adata1.obs['kidney_anno']=''
+<div class="clipboard-copy-txt" id="cell-20">adata1.obs['kidney_anno']=''
 adata1.obs.loc[query_adata.obs.index,'kidney_anno']=query_adata.obs['annotation']</div>
 </div>
 </div>
@@ -2621,7 +2648,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [18]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
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@@ -2634,7 +2661,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="highlight-ipynb hl-python"><pre><span></span><span class="n">sc</span><span class="o">.</span><span class="n">pl</span><span class="o">.</span><span class="n">spatial</span><span class="p">(</span><span class="n">adata1</span><span class="p">,</span> <span class="n">color</span><span class="o">=</span><span class="s1">'kidney_anno'</span><span class="p">,</span> <span class="n">spot_size</span><span class="o">=</span><span class="mi">3</span><span class="p">,</span>
              <span class="n">palette</span><span class="o">=</span><span class="p">[</span><span class="s1">'#F5F5F5'</span><span class="p">,</span><span class="s1">'#ff7f0e'</span><span class="p">,</span> <span class="s1">'green'</span><span class="p">,])</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-20">sc.pl.spatial(adata1, color='kidney_anno', spot_size=3,
+<div class="clipboard-copy-txt" id="cell-21">sc.pl.spatial(adata1, color='kidney_anno', spot_size=3,
              palette=['#F5F5F5','#ff7f0e', 'green',])</div>
 </div>
 </div>
@@ -2673,7 +2700,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [22]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
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@@ -2693,7 +2720,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <span class="n">ov</span><span class="o">.</span><span class="n">utils</span><span class="o">.</span><span class="n">cluster</span><span class="p">(</span><span class="n">kidney_lineage_ad</span><span class="p">,</span><span class="n">method</span><span class="o">=</span><span class="s1">'leiden'</span><span class="p">,</span><span class="n">resolution</span><span class="o">=</span><span class="mi">1</span><span class="p">)</span>
 <span class="n">ov</span><span class="o">.</span><span class="n">pp</span><span class="o">.</span><span class="n">umap</span><span class="p">(</span><span class="n">kidney_lineage_ad</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-21">kidney_lineage_ad=sc.concat([query_adata,adata2[adata2.obs['annotation']=='Kidney']],merge='same')
+<div class="clipboard-copy-txt" id="cell-22">kidney_lineage_ad=sc.concat([query_adata,adata2[adata2.obs['annotation']=='Kidney']],merge='same')
 kidney_lineage_ad=ov.pp.preprocess(kidney_lineage_ad,mode='shiftlog|pearson',n_HVGs=3000,target_sum=1e4)
 kidney_lineage_ad.raw = kidney_lineage_ad
 kidney_lineage_ad = kidney_lineage_ad[:, kidney_lineage_ad.var.highly_variable_features]
@@ -2757,7 +2784,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [23]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
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@@ -2771,7 +2798,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                <span class="n">color</span><span class="o">=</span><span class="p">[</span><span class="s1">'annotation'</span><span class="p">,</span><span class="s1">'week'</span><span class="p">,</span><span class="s1">'leiden'</span><span class="p">],</span>
                <span class="n">frameon</span><span class="o">=</span><span class="s1">'small'</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-22">ov.pl.embedding(kidney_lineage_ad,basis='X_umap',
+<div class="clipboard-copy-txt" id="cell-23">ov.pl.embedding(kidney_lineage_ad,basis='X_umap',
                color=['annotation','week','leiden'],
                frameon='small')</div>
 </div>
@@ -2800,7 +2827,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [25]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
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@@ -2816,7 +2843,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                          <span class="s1">'kidney'</span><span class="p">:[</span><span class="s1">'Wt1'</span><span class="p">,</span><span class="s1">'Wnt4'</span><span class="p">,</span><span class="s1">'Nr2f2'</span><span class="p">,</span><span class="s1">'Dach1'</span><span class="p">,</span><span class="s1">'Cd44'</span><span class="p">]}</span> <span class="p">,</span>
               <span class="s1">'leiden'</span><span class="p">,</span><span class="n">dendrogram</span><span class="o">=</span><span class="kc">False</span><span class="p">,</span><span class="n">colorbar_title</span><span class="o">=</span><span class="s1">'Expression'</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-23"># Nphs1 https://www.nature.com/articles/s41467-021-22266-1
+<div class="clipboard-copy-txt" id="cell-24"># Nphs1 https://www.nature.com/articles/s41467-021-22266-1
 sc.pl.dotplot(kidney_lineage_ad,{'nephron progenitors':['Wnt9b','Osr1','Nphs1','Lhx1','Pax2','Pax8'],
                          'metanephric':['Eya1','Shisa3','Foxc1'], 
                          'kidney':['Wt1','Wnt4','Nr2f2','Dach1','Cd44']} ,
@@ -2847,7 +2874,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [26]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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@@ -2862,7 +2889,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <span class="n">kidney_lineage_ad</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">loc</span><span class="p">[</span><span class="n">kidney_lineage_ad</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">leiden</span><span class="o">.</span><span class="n">isin</span><span class="p">([</span><span class="s1">'2'</span><span class="p">,</span><span class="s1">'3'</span><span class="p">,</span><span class="s1">'1'</span><span class="p">,</span><span class="s1">'5'</span><span class="p">]),</span><span class="s1">'re_anno'</span><span class="p">]</span> <span class="o">=</span> <span class="s1">'Metanephron progenitors (E11.5)'</span>
 <span class="n">kidney_lineage_ad</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">loc</span><span class="p">[</span><span class="n">kidney_lineage_ad</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">leiden</span><span class="o">==</span><span class="s1">'0'</span><span class="p">,</span><span class="s1">'re_anno'</span><span class="p">]</span> <span class="o">=</span> <span class="s1">'Kidney (E12.5)'</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-24">kidney_lineage_ad.obs['re_anno'] = 'Unknown'
+<div class="clipboard-copy-txt" id="cell-25">kidney_lineage_ad.obs['re_anno'] = 'Unknown'
 kidney_lineage_ad.obs.loc[kidney_lineage_ad.obs.leiden.isin(['4']),'re_anno'] = 'Nephron progenitors (E11.5)'
 kidney_lineage_ad.obs.loc[kidney_lineage_ad.obs.leiden.isin(['2','3','1','5']),'re_anno'] = 'Metanephron progenitors (E11.5)'
 kidney_lineage_ad.obs.loc[kidney_lineage_ad.obs.leiden=='0','re_anno'] = 'Kidney (E12.5)'</div>
@@ -2880,7 +2907,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
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@@ -2896,7 +2923,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                <span class="n">color</span><span class="o">=</span><span class="p">[</span><span class="s1">'annotation'</span><span class="p">,</span><span class="s1">'re_anno'</span><span class="p">],</span>
                <span class="n">frameon</span><span class="o">=</span><span class="s1">'small'</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-25"># kidney_all = kidney_all[kidney_all.obs.leiden!='3',:]
+<div class="clipboard-copy-txt" id="cell-26"># kidney_all = kidney_all[kidney_all.obs.leiden!='3',:]
 kidney_lineage_ad.obs.leiden = list(kidney_lineage_ad.obs.leiden)
 ov.pl.embedding(kidney_lineage_ad,basis='X_umap',
                color=['annotation','re_anno'],
@@ -2927,7 +2954,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [29]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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-<clipboard-copy for="cell-26">
+<clipboard-copy for="cell-27">
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@@ -2940,7 +2967,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="highlight-ipynb hl-python"><pre><span></span><span class="n">adata1</span><span class="o">.</span><span class="n">obs</span><span class="p">[</span><span class="s1">'kidney_anno'</span><span class="p">]</span><span class="o">=</span><span class="s1">''</span>
 <span class="n">adata1</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">loc</span><span class="p">[</span><span class="n">kidney_lineage_ad</span><span class="p">[</span><span class="n">kidney_lineage_ad</span><span class="o">.</span><span class="n">obs</span><span class="p">[</span><span class="s1">'week'</span><span class="p">]</span><span class="o">==</span><span class="s1">'E11.5'</span><span class="p">]</span><span class="o">.</span><span class="n">obs</span><span class="o">.</span><span class="n">index</span><span class="p">,</span><span class="s1">'kidney_anno'</span><span class="p">]</span><span class="o">=</span><span class="n">kidney_lineage_ad</span><span class="p">[</span><span class="n">kidney_lineage_ad</span><span class="o">.</span><span class="n">obs</span><span class="p">[</span><span class="s1">'week'</span><span class="p">]</span><span class="o">==</span><span class="s1">'E11.5'</span><span class="p">]</span><span class="o">.</span><span class="n">obs</span><span class="p">[</span><span class="s1">'re_anno'</span><span class="p">]</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-26">adata1.obs['kidney_anno']=''
+<div class="clipboard-copy-txt" id="cell-27">adata1.obs['kidney_anno']=''
 adata1.obs.loc[kidney_lineage_ad[kidney_lineage_ad.obs['week']=='E11.5'].obs.index,'kidney_anno']=kidney_lineage_ad[kidney_lineage_ad.obs['week']=='E11.5'].obs['re_anno']</div>
 </div>
 </div>
@@ -2956,7 +2983,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [41]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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-<clipboard-copy for="cell-27">
+<clipboard-copy for="cell-28">
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@@ -2971,7 +2998,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <span class="n">sc</span><span class="o">.</span><span class="n">pl</span><span class="o">.</span><span class="n">spatial</span><span class="p">(</span><span class="n">adata1</span><span class="p">,</span> <span class="n">color</span><span class="o">=</span><span class="s1">'kidney_anno'</span><span class="p">,</span> <span class="n">spot_size</span><span class="o">=</span><span class="mf">1.5</span><span class="p">,</span>
              <span class="n">palette</span><span class="o">=</span><span class="p">[</span><span class="s1">'#F5F5F5'</span><span class="p">,</span><span class="s1">'#ff7f0e'</span><span class="p">,</span> <span class="s1">'green'</span><span class="p">,],</span><span class="n">show</span><span class="o">=</span><span class="kc">False</span><span class="p">,</span><span class="n">ax</span><span class="o">=</span><span class="n">ax</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-27">import matplotlib.pyplot as plt
+<div class="clipboard-copy-txt" id="cell-28">import matplotlib.pyplot as plt
 fig, ax = plt.subplots(1, 1, figsize=(8, 8))
 sc.pl.spatial(adata1, color='kidney_anno', spot_size=1.5,
              palette=['#F5F5F5','#ff7f0e', 'green',],show=False,ax=ax)</div>
@@ -3018,7 +3045,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [42]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
 <div class="zeroclipboard-container">
-<clipboard-copy for="cell-28">
+<clipboard-copy for="cell-29">
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@@ -3040,7 +3067,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                    <span class="n">pval_threshold</span><span class="o">=</span><span class="mf">0.05</span><span class="p">,</span>
                    <span class="n">logp_max</span><span class="o">=</span><span class="mi">10</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-28">test_adata=kidney_lineage_ad
+<div class="clipboard-copy-txt" id="cell-29">test_adata=kidney_lineage_ad
 dds=ov.bulk.pyDEG(test_adata.to_df(layer='lognorm').T)
 dds.drop_duplicates_index()
 print('... drop_duplicates_index success')
@@ -3079,7 +3106,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [43]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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+<clipboard-copy for="cell-30">
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@@ -3092,7 +3119,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="highlight-ipynb hl-python"><pre><span></span><span class="n">dds</span><span class="o">.</span><span class="n">plot_volcano</span><span class="p">(</span><span class="n">title</span><span class="o">=</span><span class="s1">'DEG Analysis'</span><span class="p">,</span><span class="n">figsize</span><span class="o">=</span><span class="p">(</span><span class="mi">4</span><span class="p">,</span><span class="mi">4</span><span class="p">),</span>
                  <span class="n">plot_genes_num</span><span class="o">=</span><span class="mi">8</span><span class="p">,</span><span class="n">plot_genes_fontsize</span><span class="o">=</span><span class="mi">12</span><span class="p">,)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-29">dds.plot_volcano(title='DEG Analysis',figsize=(4,4),
+<div class="clipboard-copy-txt" id="cell-30">dds.plot_volcano(title='DEG Analysis',figsize=(4,4),
                  plot_genes_num=8,plot_genes_fontsize=12,)</div>
 </div>
 </div>
@@ -3126,7 +3153,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [52]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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+<clipboard-copy for="cell-31">
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@@ -3140,7 +3167,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <span class="n">down_gene</span><span class="o">=</span><span class="n">dds</span><span class="o">.</span><span class="n">result</span><span class="o">.</span><span class="n">loc</span><span class="p">[</span><span class="n">dds</span><span class="o">.</span><span class="n">result</span><span class="p">[</span><span class="s1">'sig'</span><span class="p">]</span><span class="o">==</span><span class="s1">'down'</span><span class="p">]</span><span class="o">.</span><span class="n">sort_values</span><span class="p">(</span><span class="s1">'qvalue'</span><span class="p">)[:</span><span class="mi">3</span><span class="p">]</span><span class="o">.</span><span class="n">index</span><span class="o">.</span><span class="n">tolist</span><span class="p">()</span>
 <span class="n">deg_gene</span><span class="o">=</span><span class="n">up_gene</span><span class="o">+</span><span class="n">down_gene</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-30">up_gene=dds.result.loc[dds.result['sig']=='up'].sort_values('qvalue')[:3].index.tolist()
+<div class="clipboard-copy-txt" id="cell-31">up_gene=dds.result.loc[dds.result['sig']=='up'].sort_values('qvalue')[:3].index.tolist()
 down_gene=dds.result.loc[dds.result['sig']=='down'].sort_values('qvalue')[:3].index.tolist()
 deg_gene=up_gene+down_gene</div>
 </div>
@@ -3157,7 +3184,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [53]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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+<clipboard-copy for="cell-32">
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@@ -3170,7 +3197,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="highlight-ipynb hl-python"><pre><span></span><span class="n">sc</span><span class="o">.</span><span class="n">pl</span><span class="o">.</span><span class="n">dotplot</span><span class="p">(</span><span class="n">kidney_lineage_ad</span><span class="p">,</span><span class="n">deg_gene</span><span class="p">,</span>
              <span class="n">groupby</span><span class="o">=</span><span class="s1">'re_anno'</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-31">sc.pl.dotplot(kidney_lineage_ad,deg_gene,
+<div class="clipboard-copy-txt" id="cell-32">sc.pl.dotplot(kidney_lineage_ad,deg_gene,
              groupby='re_anno')</div>
 </div>
 </div>
@@ -3209,7 +3236,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
 <div class="jp-InputPrompt jp-InputArea-prompt">In [55]:</div><div class="jp-CodeMirrorEditor jp-Editor jp-InputArea-editor" data-type="inline">
 <div class="CodeMirror cm-s-jupyter">
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+<clipboard-copy for="cell-33">
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@@ -3226,7 +3253,7 @@ <h2 id="focus-on-developing-kidney">Focus on developing Kidney<a class="anchor-l
                                 <span class="n">cmap</span><span class="o">=</span><span class="s1">'RdBu_r'</span><span class="p">,</span><span class="n">key</span><span class="o">=</span><span class="s1">'re_anno_ttest'</span><span class="p">,</span>
                                 <span class="n">standard_scale</span><span class="o">=</span><span class="s1">'var'</span><span class="p">,</span><span class="n">n_genes</span><span class="o">=</span><span class="mi">3</span><span class="p">)</span>
 </pre></div>
-<div class="clipboard-copy-txt" id="cell-32">sc.tl.dendrogram(kidney_lineage_ad,'re_anno',use_rep='scaled|original|X_pca')
+<div class="clipboard-copy-txt" id="cell-33">sc.tl.dendrogram(kidney_lineage_ad,'re_anno',use_rep='scaled|original|X_pca')
 sc.tl.rank_genes_groups(kidney_lineage_ad, 're_anno', use_rep='scaled|original|X_pca',
                         method='t-test',use_raw=False,key_added='re_anno_ttest')
 sc.pl.rank_genes_groups_dotplot(kidney_lineage_ad,groupby='re_anno',
diff --git a/sitemap.xml.gz b/sitemap.xml.gz
index 3de739d..3a95c01 100644
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