From c8e42b32076a48170a6554e2c253eee1c1559d9a Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 22 Apr 2024 12:57:00 +1200
Subject: [PATCH 01/76] Fixed a bug which caused NCBI_FCS_GX to not resume

---
 CHANGELOG.md                      | 15 +++++++++++++++
 CITATION.cff                      |  2 +-
 nextflow.config                   |  2 +-
 subworkflows/local/ncbi_fcs_gx.nf |  3 +++
 4 files changed, 20 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index dc2e51c8..47334b2c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,21 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## 2.0.0 - [22-April-2024]
+
+### `Added`
+
+### `Fixed`
+
+1. Fixed a bug which caused NCBI_FCS_GX to not resume
+
+### `Dependencies`
+
+1. NextFlow!>=23.04.0
+2. nf-validation@1.1.3
+
+### `Deprecated`
+
 ## 1.4 - [04-Mar-2024]
 
 ### `Added`
diff --git a/CITATION.cff b/CITATION.cff
index efb1b209..23125eae 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -21,7 +21,7 @@ authors:
   - family-names: "Deng"
     given-names: "Cecilia"
 title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality"
-version: 1.4
+version: 2.0.0
 date-released: 2024-02-12
 url: "https://github.com/Plant-Food-Research-Open/assembly_qc"
 doi: 10.5281/zenodo.10647870
diff --git a/nextflow.config b/nextflow.config
index f0ee1b5b..c110c029 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -262,7 +262,7 @@ manifest {
     description     = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '1.4'
+    version         = '2.0.0'
     doi             = '10.5281/zenodo.10647870'
 }
 
diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf
index cc54292f..0c442a6e 100644
--- a/subworkflows/local/ncbi_fcs_gx.nf
+++ b/subworkflows/local/ncbi_fcs_gx.nf
@@ -16,6 +16,9 @@ workflow NCBI_FCS_GX {
 
     ch_all_samples          = NCBI_FCS_GX_SETUP_SAMPLE.out.fsata
                             | collect
+                            | map {
+                                it.sort(false)
+                            }
 
     ch_versions             = ch_versions.mix(NCBI_FCS_GX_SETUP_SAMPLE.out.versions.first())
 

From f50728b295cd2b4e930f41f4d2c2ef8f830632c3 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 22 Apr 2024 13:08:49 +1200
Subject: [PATCH 02/76] Updated modules and sub-workflows

---
 .editorconfig                                 | 27 +++++++++++++
 CHANGELOG.md                                  |  2 +
 modules.json                                  | 40 +++++++++----------
 modules/nf-core/fastp/main.nf                 |  2 +-
 modules/nf-core/fastp/tests/main.nf.test      | 18 +++++----
 modules/nf-core/fastp/tests/main.nf.test.snap | 10 ++---
 .../fastp/tests/nextflow.interleaved.config   |  5 +++
 ...low.config => nextflow.save_failed.config} |  3 +-
 modules/nf-core/fastqc/main.nf                |  6 +++
 modules/nf-core/untar/tests/main.nf.test      |  8 ----
 modules/nf-core/untar/tests/main.nf.test.snap | 12 +++++-
 modules/pfr/bwa/index/tests/main.nf.test      |  2 +-
 modules/pfr/bwa/mem/tests/main.nf.test        |  2 +-
 modules/pfr/cat/cat/tests/main.nf.test        |  2 +-
 .../tests/main.nf.test                        |  2 +-
 .../custom/restoregffids/tests/main.nf.test   |  2 +-
 .../custom/shortenfastaids/tests/main.nf.test |  2 +-
 modules/pfr/gt/gff3/tests/main.nf.test        |  2 +-
 .../pfr/gt/gff3validator/tests/main.nf.test   |  2 +-
 modules/pfr/gt/stat/tests/main.nf.test        |  2 +-
 modules/pfr/ltrfinder/tests/main.nf.test      |  2 +-
 modules/pfr/ltrharvest/tests/main.nf.test     |  2 +-
 .../pfr/ltrretriever/lai/tests/main.nf.test   |  2 +-
 .../ltrretriever/tests/main.nf.test           |  2 +-
 modules/pfr/samblaster/tests/main.nf.test     |  2 +-
 .../fasta_ltrretriever_lai/tests/main.nf.test |  2 +-
 .../tests/main.nf.test                        |  2 +-
 .../pfr/gff3_validate/tests/main.nf.test      |  2 +-
 28 files changed, 104 insertions(+), 63 deletions(-)
 create mode 100644 modules/nf-core/fastp/tests/nextflow.interleaved.config
 rename modules/nf-core/fastp/tests/{nextflow.config => nextflow.save_failed.config} (50%)

diff --git a/.editorconfig b/.editorconfig
index 8719a7f9..eb77d1dd 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -20,6 +20,33 @@ trim_trailing_whitespace = unset
 indent_style = unset
 indent_size = unset
 
+# These files are edited and tested upstream in nf-core/subworkflows
+[/subworkflows/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+indent_size = unset
+
+# These files are edited and tested upstream in pfr/modules
+[/modules/pfr/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+indent_size = unset
+
+# These files are edited and tested upstream in pfr/subworkflows
+[/subworkflows/pfr/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+indent_size = unset
+
 [/assets/email*]
 indent_size = unset
 
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 47334b2c..9a3aa939 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### `Added`
 
+1. Updated modules and sub-workflows
+
 ### `Fixed`
 
 1. Fixed a bug which caused NCBI_FCS_GX to not resume
diff --git a/modules.json b/modules.json
index a25581b4..d817ded9 100644
--- a/modules.json
+++ b/modules.json
@@ -7,72 +7,72 @@
                 "pfr": {
                     "bwa/index": {
                         "branch": "main",
-                        "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "bwa/mem": {
                         "branch": "main",
-                        "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "cat/cat": {
                         "branch": "main",
-                        "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "custom/checkgff3fastacorrespondence": {
                         "branch": "main",
-                        "git_sha": "1a76f884082c786760559c462063a5d1de94ca83",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["gff3_validate"]
                     },
                     "custom/restoregffids": {
                         "branch": "main",
-                        "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "custom/shortenfastaids": {
                         "branch": "main",
-                        "git_sha": "5e0e41b51d7fc7f68ae43692b6fe19b95d7f3a8c",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "gt/gff3": {
                         "branch": "main",
-                        "git_sha": "bfa4874d3942bdff70cb8df17322834125cafb28",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["gff3_validate"]
                     },
                     "gt/gff3validator": {
                         "branch": "main",
-                        "git_sha": "889b9b57b611dcb063594608c2a222c928327cba",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["gff3_validate"]
                     },
                     "gt/stat": {
                         "branch": "main",
-                        "git_sha": "cb5fb0be78a98fd1e32b7c90d6adf8c3bf44133e",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["modules"]
                     },
                     "ltrfinder": {
                         "branch": "main",
-                        "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "ltrharvest": {
                         "branch": "main",
-                        "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "ltrretriever/lai": {
                         "branch": "main",
-                        "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "ltrretriever/ltrretriever": {
                         "branch": "main",
-                        "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "samblaster": {
                         "branch": "main",
-                        "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
                     }
                 }
@@ -81,17 +81,17 @@
                 "pfr": {
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
-                        "git_sha": "f148f5384395618fc706b6e2f059bd1ce037d06c",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["subworkflows"]
                     },
                     "fastq_bwa_mem_samblaster": {
                         "branch": "main",
-                        "git_sha": "9639ac9a556898d0f0e8592bff24585c33326458",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["subworkflows"]
                     },
                     "gff3_validate": {
                         "branch": "main",
-                        "git_sha": "f9b96bf8142a01f0649ff90570fb10aa973504b9",
+                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["subworkflows"]
                     }
                 }
@@ -112,12 +112,12 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56",
+                        "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
                         "installed_by": ["fastq_trim_fastp_fastqc"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
+                        "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
                         "installed_by": ["fastq_trim_fastp_fastqc"]
                     },
                     "gunzip": {
@@ -152,7 +152,7 @@
                     },
                     "untar": {
                         "branch": "master",
-                        "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index 2a3b679e..4fc19b74 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -29,7 +29,7 @@ process FASTP {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : ""
-    def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
+    def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--failed_out ${prefix}.paired.fail.fastq.gz --unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
     // Added soft-links to original fastqs for consistent naming in MultiQC
     // Use single ended for interleaved. Add --interleaved_in in config.
     if ( task.ext.args?.contains('--interleaved_in') ) {
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index 9b3f9a38..6f1f4897 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -251,7 +251,8 @@ nextflow_process {
     }
 
     test("fastp test_fastp_interleaved") {
-        config './nextflow.config'
+
+        config './nextflow.interleaved.config'
         when {
             params {
                 outdir   = "$outputDir"
@@ -277,7 +278,7 @@ nextflow_process {
             def html_text  = [ "Q20 bases:</td><td class='col2'>25.719000 K (93.033098%)",
 									"paired end (151 cycles + 151 cycles)"]
             def log_text   = [ "Q20 bases: 12922(92.9841%)",
-									"reads passed filter: 198"]
+									"reads passed filter: 162"]
             def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
 									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
 									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
@@ -321,7 +322,7 @@ nextflow_process {
 
         options '-stub'
 
-        config './nextflow.config'
+        config './nextflow.interleaved.config'
         when {
             params {
                 outdir   = "$outputDir"
@@ -427,6 +428,7 @@ nextflow_process {
 
     test("test_fastp_paired_end_trim_fail") {
 
+        config './nextflow.save_failed.config'
         when {
             params {
                 outdir   = "$outputDir"
@@ -454,7 +456,7 @@ nextflow_process {
 									"The input has little adapter percentage (~0.000000%), probably it's trimmed before."]
             def log_text            = [ "No adapter detected for read1",
 									"Q30 bases: 12281(88.3716%)"]
-            def json_text           = ['"passed_filter_reads": 198']
+            def json_text           = ['"passed_filter_reads": 162']
             def read1_lines         = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
 									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
 									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
@@ -467,9 +469,9 @@ nextflow_process {
 									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/2",
 									"GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGT",
 									"AAAAA6EEAEEEEEAEEAEEAEEEEEEA6EEEEAEEAEEEEE6EEEEEEAEEEEA///A<<EEEEEEEEEAEEEEEE"]
-            def failed_read2_lines  = ["@ERR5069949.885966 NS500628:121:HK3MMAFX2:4:11610:19682:20132/2",
-									"CTTAGGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAA",
-									"A//AA6EEAEEEEE6EEE/EEA/EA///AAE/EAEEEAE6AE/E/E/EEAAE/EAA/E/E/<EA//E/6"]
+            def failed_read2_lines  = ["@ERR5069949.973930 NS500628:121:HK3MMAFX2:2:21105:5548:5659/2",
+									"CCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAG",
+									"AAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEAE<EAEEEEEEAEEAEE"]
             // def failed_read1_lines = path(process.out.reads_fail.get(0).get(1).get(0)).linesGzip is empty file
             assertAll(
                 { assert process.success },
@@ -483,7 +485,7 @@ nextflow_process {
                     }
                 },
                 { failed_read2_lines.each { failed_read2_line ->
-                    { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) }
+                    { assert path(process.out.reads_fail.get(0).get(1).get(2)).linesGzip.contains(failed_read2_line) }
                     }
                 },
                 { html_text.each { html_part ->
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index b4c0e1dd..3e876288 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -7,7 +7,7 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4"
+                    "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22"
                 ]
             ]
         ],
@@ -15,7 +15,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-01-17T18:08:06.123035"
+        "timestamp": "2024-03-18T16:19:15.063001"
     },
     "test_fastp_paired_end_merged-for_stub_match": {
         "content": [
@@ -65,7 +65,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-01-17T18:06:00.223817"
+        "timestamp": "2024-03-18T16:18:43.526412"
     },
     "versions_paired_end": {
         "content": [
@@ -112,7 +112,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-01T12:03:37.827323085"
+        "timestamp": "2024-03-18T16:19:15.111894"
     },
     "test_fastp_paired_end_merged_match": {
         "content": [
@@ -283,7 +283,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-01T11:57:30.791982648"
+        "timestamp": "2024-03-18T16:18:43.580336"
     },
     "versions_paired_end_merged_adapterlist": {
         "content": [
diff --git a/modules/nf-core/fastp/tests/nextflow.interleaved.config b/modules/nf-core/fastp/tests/nextflow.interleaved.config
new file mode 100644
index 00000000..4be8dbd2
--- /dev/null
+++ b/modules/nf-core/fastp/tests/nextflow.interleaved.config
@@ -0,0 +1,5 @@
+process {
+    withName: FASTP {
+        ext.args = "--interleaved_in -e 30"
+    }
+}
diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.save_failed.config
similarity index 50%
rename from modules/nf-core/fastp/tests/nextflow.config
rename to modules/nf-core/fastp/tests/nextflow.save_failed.config
index 0f7849ad..53b61b0c 100644
--- a/modules/nf-core/fastp/tests/nextflow.config
+++ b/modules/nf-core/fastp/tests/nextflow.save_failed.config
@@ -1,6 +1,5 @@
 process {
-
     withName: FASTP {
-        ext.args = "--interleaved_in"
+        ext.args = "-e 30"
     }
 }
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 9e19a74c..d79f1c86 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -25,6 +25,11 @@ process FASTQC {
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
     def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB')
+    // FastQC memory value allowed range (100 - 10000)
+    def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
+
     """
     printf "%s %s\\n" $rename_to | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
@@ -33,6 +38,7 @@ process FASTQC {
     fastqc \\
         $args \\
         --threads $task.cpus \\
+        --memory $fastqc_memory \\
         $renamed_files
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test
index 679e83c7..2a7c97bf 100644
--- a/modules/nf-core/untar/tests/main.nf.test
+++ b/modules/nf-core/untar/tests/main.nf.test
@@ -3,17 +3,12 @@ nextflow_process {
     name "Test Process UNTAR"
     script "../main.nf"
     process "UNTAR"
-
     tag "modules"
     tag "modules_nfcore"
     tag "untar"
-
     test("test_untar") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ]
@@ -33,9 +28,6 @@ nextflow_process {
     test("test_untar_onlyfiles") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ]
diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap
index ace42576..64550292 100644
--- a/modules/nf-core/untar/tests/main.nf.test.snap
+++ b/modules/nf-core/untar/tests/main.nf.test.snap
@@ -12,7 +12,11 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-18T11:56:46.878844"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T11:49:41.320643"
     },
     "test_untar": {
         "content": [
@@ -29,6 +33,10 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-18T11:56:08.16574"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T11:49:33.795172"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/bwa/index/tests/main.nf.test b/modules/pfr/bwa/index/tests/main.nf.test
index af33e73c..b0d2c726 100644
--- a/modules/pfr/bwa/index/tests/main.nf.test
+++ b/modules/pfr/bwa/index/tests/main.nf.test
@@ -30,4 +30,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/bwa/mem/tests/main.nf.test b/modules/pfr/bwa/mem/tests/main.nf.test
index 2696e4bf..804f23f7 100644
--- a/modules/pfr/bwa/mem/tests/main.nf.test
+++ b/modules/pfr/bwa/mem/tests/main.nf.test
@@ -170,4 +170,4 @@ nextflow_process {
         }
 
     }
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/cat/cat/tests/main.nf.test b/modules/pfr/cat/cat/tests/main.nf.test
index fcee2d19..0ac8549e 100644
--- a/modules/pfr/cat/cat/tests/main.nf.test
+++ b/modules/pfr/cat/cat/tests/main.nf.test
@@ -175,4 +175,4 @@ nextflow_process {
             )
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test
index 91578e5b..a45992c2 100644
--- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test
+++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test
@@ -67,4 +67,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test b/modules/pfr/custom/restoregffids/tests/main.nf.test
index 521b9248..cc374b76 100644
--- a/modules/pfr/custom/restoregffids/tests/main.nf.test
+++ b/modules/pfr/custom/restoregffids/tests/main.nf.test
@@ -60,4 +60,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test b/modules/pfr/custom/shortenfastaids/tests/main.nf.test
index dc46bae5..efff639e 100644
--- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test
+++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test
@@ -128,4 +128,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/gt/gff3/tests/main.nf.test b/modules/pfr/gt/gff3/tests/main.nf.test
index cb44bc8f..b57439ef 100644
--- a/modules/pfr/gt/gff3/tests/main.nf.test
+++ b/modules/pfr/gt/gff3/tests/main.nf.test
@@ -58,4 +58,4 @@ nextflow_process {
         }
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test b/modules/pfr/gt/gff3validator/tests/main.nf.test
index 1b99e551..9addd7d8 100644
--- a/modules/pfr/gt/gff3validator/tests/main.nf.test
+++ b/modules/pfr/gt/gff3validator/tests/main.nf.test
@@ -60,4 +60,4 @@ nextflow_process {
         }
 
     }
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/gt/stat/tests/main.nf.test b/modules/pfr/gt/stat/tests/main.nf.test
index 57f5992c..8736d013 100644
--- a/modules/pfr/gt/stat/tests/main.nf.test
+++ b/modules/pfr/gt/stat/tests/main.nf.test
@@ -34,4 +34,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/ltrfinder/tests/main.nf.test b/modules/pfr/ltrfinder/tests/main.nf.test
index 447ce34d..361e7324 100644
--- a/modules/pfr/ltrfinder/tests/main.nf.test
+++ b/modules/pfr/ltrfinder/tests/main.nf.test
@@ -69,4 +69,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/ltrharvest/tests/main.nf.test b/modules/pfr/ltrharvest/tests/main.nf.test
index 9226bc0c..76b49476 100644
--- a/modules/pfr/ltrharvest/tests/main.nf.test
+++ b/modules/pfr/ltrharvest/tests/main.nf.test
@@ -57,4 +57,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/ltrretriever/lai/tests/main.nf.test b/modules/pfr/ltrretriever/lai/tests/main.nf.test
index df7db2cf..a617811b 100644
--- a/modules/pfr/ltrretriever/lai/tests/main.nf.test
+++ b/modules/pfr/ltrretriever/lai/tests/main.nf.test
@@ -163,4 +163,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test b/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test
index f6ab43db..e8e13a60 100644
--- a/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test
+++ b/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test
@@ -130,4 +130,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/modules/pfr/samblaster/tests/main.nf.test b/modules/pfr/samblaster/tests/main.nf.test
index 01794307..06bb9162 100644
--- a/modules/pfr/samblaster/tests/main.nf.test
+++ b/modules/pfr/samblaster/tests/main.nf.test
@@ -54,4 +54,4 @@ nextflow_process {
 
     }
 
-}
+}
\ No newline at end of file
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
index 7ba88ab6..2f31d1d1 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
@@ -181,4 +181,4 @@ nextflow_workflow {
             )
         }
     }
-}
+}
\ No newline at end of file
diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test
index 1e279e48..9c3e2b01 100644
--- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test
+++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test
@@ -41,4 +41,4 @@ nextflow_workflow {
             )
         }
     }
-}
+}
\ No newline at end of file
diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test b/subworkflows/pfr/gff3_validate/tests/main.nf.test
index e71712b8..14c33ae2 100644
--- a/subworkflows/pfr/gff3_validate/tests/main.nf.test
+++ b/subworkflows/pfr/gff3_validate/tests/main.nf.test
@@ -83,4 +83,4 @@ nextflow_workflow {
             )
         }
     }
-}
+}
\ No newline at end of file

From 15cac9d16d3020a3616b640b2d7ab6e31fe2444e Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 22 Apr 2024 13:21:35 +1200
Subject: [PATCH 03/76] Template update for nf-core/tools version 2.13.2.dev0

---
 .devcontainer/devcontainer.json               |  10 +-
 .editorconfig                                 |   9 +-
 .github/CONTRIBUTING.md                       |   9 +-
 .github/ISSUE_TEMPLATE/bug_report.yml         | 100 ++--
 .github/PULL_REQUEST_TEMPLATE.md              |   2 +-
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/ci.yml                      |   7 +-
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  37 +-
 .github/workflows/fix-linting.yml             |  59 ++-
 .github/workflows/linting.yml                 |  18 +-
 .github/workflows/linting_comment.yml         |   4 +-
 .github/workflows/release-announcements.yml   |  11 +-
 .gitpod.yml                                   |   6 +-
 .nf-core.yml                                  |  31 +-
 .pre-commit-config.yaml                       |   5 +-
 CHANGELOG.md                                  |   2 +-
 LICENSE                                       |   2 +-
 README.md                                     |   9 +-
 assets/email_template.html                    |   2 +-
 assets/multiqc_config.yml                     |   6 +-
 assets/schema_input.json                      |  21 +-
 bin/check_samplesheet.py                      | 259 -----------
 conf/base.config                              |   3 -
 conf/modules.config                           |  16 -
 lib/NfcoreTemplate.groovy                     | 351 --------------
 lib/Utils.groovy                              |  47 --
 lib/WorkflowAssemblyqc.groovy                 | 122 -----
 lib/WorkflowMain.groovy                       |  63 ---
 lib/nfcore_external_java_deps.jar             | Bin 2291171 -> 0 bytes
 main.nf                                       | 100 ++--
 modules.json                                  |  28 +-
 modules/local/samplesheet_check.nf            |  31 --
 .../dumpsoftwareversions/environment.yml      |   7 -
 .../custom/dumpsoftwareversions/main.nf       |  24 -
 .../custom/dumpsoftwareversions/meta.yml      |  37 --
 .../templates/dumpsoftwareversions.py         | 102 ----
 .../dumpsoftwareversions/tests/main.nf.test   |  38 --
 .../tests/main.nf.test.snap                   |  27 --
 .../dumpsoftwareversions/tests/tags.yml       |   2 -
 modules/nf-core/fastqc/tests/main.nf.test     | 265 +++++++----
 .../nf-core/fastqc/tests/main.nf.test.snap    |  82 +++-
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   6 +-
 modules/nf-core/multiqc/meta.yml              |   1 -
 modules/nf-core/multiqc/tests/main.nf.test    |  53 ++-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  41 ++
 nextflow.config                               |  12 +-
 nextflow_schema.json                          |   3 +-
 pyproject.toml                                |  13 -
 subworkflows/local/input_check.nf             |  44 --
 .../utils_nfcore_assemblyqc_pipeline/main.nf  | 255 ++++++++++
 .../nf-core/utils_nextflow_pipeline/main.nf   | 126 +++++
 .../nf-core/utils_nextflow_pipeline/meta.yml  |  38 ++
 .../tests/main.function.nf.test               |  54 +++
 .../tests/main.function.nf.test.snap          |  20 +
 .../tests/main.workflow.nf.test               | 111 +++++
 .../tests/nextflow.config                     |   9 +
 .../utils_nextflow_pipeline/tests/tags.yml    |   2 +
 .../nf-core/utils_nfcore_pipeline/main.nf     | 440 ++++++++++++++++++
 .../nf-core/utils_nfcore_pipeline/meta.yml    |  24 +
 .../tests/main.function.nf.test               | 134 ++++++
 .../tests/main.function.nf.test.snap          | 166 +++++++
 .../tests/main.workflow.nf.test               |  29 ++
 .../tests/main.workflow.nf.test.snap          |  19 +
 .../tests/nextflow.config                     |   9 +
 .../utils_nfcore_pipeline/tests/tags.yml      |   2 +
 .../nf-core/utils_nfvalidation_plugin/main.nf |  62 +++
 .../utils_nfvalidation_plugin/meta.yml        |  44 ++
 .../tests/main.nf.test                        | 200 ++++++++
 .../tests/nextflow_schema.json                |  96 ++++
 .../utils_nfvalidation_plugin/tests/tags.yml  |   2 +
 workflows/assemblyqc.nf                       | 148 +++---
 73 files changed, 2550 insertions(+), 1573 deletions(-)
 delete mode 100755 bin/check_samplesheet.py
 delete mode 100755 lib/NfcoreTemplate.groovy
 delete mode 100644 lib/Utils.groovy
 delete mode 100755 lib/WorkflowAssemblyqc.groovy
 delete mode 100755 lib/WorkflowMain.groovy
 delete mode 100644 lib/nfcore_external_java_deps.jar
 delete mode 100644 modules/local/samplesheet_check.nf
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml
 delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap
 delete mode 100644 pyproject.toml
 delete mode 100644 subworkflows/local/input_check.nf
 create mode 100644 subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
index 4ecfbfe3..b290e090 100644
--- a/.devcontainer/devcontainer.json
+++ b/.devcontainer/devcontainer.json
@@ -10,15 +10,7 @@
         "vscode": {
             // Set *default* container specific settings.json values on container create.
             "settings": {
-                "python.defaultInterpreterPath": "/opt/conda/bin/python",
-                "python.linting.enabled": true,
-                "python.linting.pylintEnabled": true,
-                "python.formatting.autopep8Path": "/opt/conda/bin/autopep8",
-                "python.formatting.yapfPath": "/opt/conda/bin/yapf",
-                "python.linting.flake8Path": "/opt/conda/bin/flake8",
-                "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle",
-                "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle",
-                "python.linting.pylintPath": "/opt/conda/bin/pylint"
+                "python.defaultInterpreterPath": "/opt/conda/bin/python"
             },
 
             // Add the IDs of extensions you want installed when the container is created.
diff --git a/.editorconfig b/.editorconfig
index 9b990088..dd9ffa53 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -18,7 +18,12 @@ end_of_line = unset
 insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
-indent_size = unset
+[/subworkflows/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
 
 [/assets/email*]
 indent_size = unset
@@ -28,5 +33,5 @@ indent_size = unset
 indent_style = unset
 
 # ignore python
-[*.{py}]
+[*.{py,md}]
 indent_style = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index ab01b3d5..0144e255 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -23,8 +23,11 @@ If you're not used to this workflow with git, you can start with some [docs from
 
 ## Tests
 
-You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to
-receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir <OUTDIR>`.
+You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command:
+
+```bash
+nf-test test --profile debug,test,docker --verbose
+```
 
 When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
 Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
@@ -82,7 +85,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`.
 
 Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
 
-The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block.
+The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block.
 
 ### Naming schemes
 
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index c22d7e64..af436aa6 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -2,53 +2,53 @@ name: Bug report
 description: Report something that is broken or incorrect
 labels: bug
 body:
-- type: textarea
-  id: description
-  attributes:
-    label: Description of the bug
-    description: A clear and concise description of what the bug is.
-  validations:
-    required: true
-- type: textarea
-  id: command_used
-  attributes:
-    label: Command used and terminal output
-    description: Steps to reproduce the behaviour. Please paste the command you used
-      to launch the pipeline and the output from your terminal.
-    render: console
-    placeholder: '$ nextflow run ...
-
-
-      Some output where something broke
-
-      '
-- type: textarea
-  id: files
-  attributes:
-    label: Relevant files
-    description: 'Please drag and drop the relevant files here. Create a `.zip` archive
-      if the extension is not allowed.
-
-      Your verbose log file `.nextflow.log` is often useful _(this is a hidden file
-      in the directory where you launched the pipeline)_ as well as custom Nextflow
-      configuration files.
-
-      '
-- type: textarea
-  id: system
-  attributes:
-    label: System information
-    description: '* Nextflow version _(eg. 23.04.0)_
-
-      * Hardware _(eg. HPC, Desktop, Cloud)_
-
-      * Executor _(eg. slurm, local, awsbatch)_
-
-      * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud,
-      or Apptainer)_
-
-      * OS _(eg. CentOS Linux, macOS, Linux Mint)_
-
-      * Version of plant-food-research-open/assemblyqc _(eg. 1.1, 1.5, 1.8.2)_
-
-      '
+  - type: textarea
+    id: description
+    attributes:
+      label: Description of the bug
+      description: A clear and concise description of what the bug is.
+    validations:
+      required: true
+  - type: textarea
+    id: command_used
+    attributes:
+      label: Command used and terminal output
+      description: Steps to reproduce the behaviour. Please paste the command you used
+        to launch the pipeline and the output from your terminal.
+      render: console
+      placeholder: "$ nextflow run ...
+
+
+        Some output where something broke
+
+        "
+  - type: textarea
+    id: files
+    attributes:
+      label: Relevant files
+      description: "Please drag and drop the relevant files here. Create a `.zip` archive
+        if the extension is not allowed.
+
+        Your verbose log file `.nextflow.log` is often useful _(this is a hidden file
+        in the directory where you launched the pipeline)_ as well as custom Nextflow
+        configuration files.
+
+        "
+  - type: textarea
+    id: system
+    attributes:
+      label: System information
+      description: "* Nextflow version _(eg. 23.04.0)_
+
+        * Hardware _(eg. HPC, Desktop, Cloud)_
+
+        * Executor _(eg. slurm, local, awsbatch)_
+
+        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud,
+        or Apptainer)_
+
+        * OS _(eg. CentOS Linux, macOS, Linux Mint)_
+
+        * Version of plant-food-research-open/assemblyqc _(eg. 1.1, 1.5, 1.8.2)_
+
+        "
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 3da295ca..86930edb 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/plant-food-r
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md)
 - [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
+- [ ] Ensure the test suite passes (`nf-test test tests/ --verbose --profile +docker`).
 - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 752ea1bf..9186934e 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -19,7 +19,7 @@ jobs:
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
-        uses: mshick/add-pr-comment@v2
+        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
         with:
           message: |
             ## This PR is against the `master` branch :x:
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 9e9668cc..ce5b32ae 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,13 +28,16 @@ jobs:
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v4
+        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
         with:
           version: "${{ matrix.NXF_VER }}"
 
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
       - name: Run pipeline with test data
         # TODO nf-core: You can customise CI pipeline run tests as required
         # For example: adding multiple test runs with different parameters
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index e37cfda5..0b6b1f27 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@v9
+      - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 47168689..2d20d644 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -6,9 +6,16 @@ name: Test successful pipeline download with 'nf-core download'
 #  - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev.
 on:
   workflow_dispatch:
+    inputs:
+      testbranch:
+        description: "The specific branch you wish to utilize for the test execution of nf-core download."
+        required: true
+        default: "dev"
   pull_request:
     types:
       - opened
+      - edited
+      - synchronize
     branches:
       - master
   pull_request_target:
@@ -20,16 +27,19 @@ env:
 
 jobs:
   download:
-    runs-on: ["self-hosted"]
+    runs-on: ubuntu-latest
     steps:
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@v5
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
-      - uses: eWaterCycle/setup-singularity@v7
+      - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
         with:
           singularity-version: 3.8.3
 
@@ -42,13 +52,13 @@ jobs:
         run: |
           echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
           echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
-          echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV}
+          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}
 
       - name: Download the pipeline
         env:
           NXF_SINGULARITY_CACHEDIR: ./
         run: |
-          nf-core download  ${{ env.REPO_LOWERCASE }} \
+          nf-core download ${{ env.REPO_LOWERCASE }} \
           --revision ${{ env.REPO_BRANCH }} \
           --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
           --compress "none" \
@@ -60,8 +70,17 @@ jobs:
       - name: Inspect download
         run: tree ./${{ env.REPOTITLE_LOWERCASE }}
 
-      - name: Run the downloaded pipeline
+      - name: Run the downloaded pipeline (stub)
+        id: stub_run_pipeline
+        continue-on-error: true
+        env:
+          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_HOME_MOUNT: true
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
+      - name: Run the downloaded pipeline (stub run not supported)
+        id: run_pipeline
+        if: ${{ job.steps.stub_run_pipeline.status == failure() }}
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results 
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 41963bec..52217b84 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -4,7 +4,7 @@ on:
     types: [created]
 
 jobs:
-  deploy:
+  fix-linting:
     # Only run if comment is on a PR with the main repo, and if it contains the magic keywords
     if: >
       contains(github.event.comment.html_url, '/pull/') &&
@@ -13,10 +13,17 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
+      # indication that the linting is being fixed
+      - name: React on comment
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: eyes
+
       # Action runs on the issue comment, so we don't get the PR by default
       # Use the gh cli to check out the PR
       - name: Checkout Pull Request
@@ -24,25 +31,59 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      - name: Set up Python 3.11
-        uses: actions/setup-python@v5
+      # Install and run pre-commit
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
-          cache: "pip"
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
 
       - name: Run pre-commit
-        run: pre-commit run --all-files || echo "status=fail" >> $GITHUB_ENV
+        id: pre-commit
+        run: pre-commit run --all-files
+        continue-on-error: true
+
+      # indication that the linting has finished
+      - name: react if linting finished succesfully
+        if: steps.pre-commit.outcome == 'success'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: "+1"
 
       - name: Commit & push changes
-        if: env.status == 'fail'
+        id: commit-and-push
+        if: steps.pre-commit.outcome == 'failure'
         run: |
           git config user.email "core@nf-co.re"
           git config user.name "nf-core-bot"
           git config push.default upstream
           git add .
           git status
-          git commit -m "[automated] Fix linting with pre-commit"
+          git commit -m "[automated] Fix code linting"
           git push
+
+      - name: react if linting errors were fixed
+        id: react-if-fixed
+        if: steps.commit-and-push.outcome == 'success'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: hooray
+
+      - name: react if linting errors were not fixed
+        if: steps.commit-and-push.outcome == 'failure'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: confused
+
+      - name: react if linting errors were not fixed
+        if: steps.commit-and-push.outcome  == 'failure'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          issue-number: ${{ github.event.issue.number }}
+          body: |
+            @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually.
+            See [CI log](https://github.com/plant-food-research-open/assemblyqc/actions/runs/${{ github.run_id }}) for more details.
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 81cd098e..a225c816 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,12 +14,12 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
-      - name: Set up Python 3.11
-        uses: actions/setup-python@v5
+      - name: Set up Python 3.12
+        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
+          python-version: "3.12"
           cache: "pip"
 
       - name: Install pre-commit
@@ -32,14 +32,14 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v4
+        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
 
       - name: Install dependencies
@@ -60,7 +60,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@v4
+        uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 147bcd10..40acc23f 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@v3
+        uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@v2
+        uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 21ac3f06..03ecfcf7 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -9,6 +9,11 @@ jobs:
   toot:
     runs-on: ubuntu-latest
     steps:
+      - name: get topics and convert to hashtags
+        id: get_topics
+        run: |
+          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')"  >> $GITHUB_OUTPUT
+
       - uses: rzr/fediverse-action@master
         with:
           access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
@@ -20,11 +25,13 @@ jobs:
 
             Please see the changelog: ${{ github.event.release.html_url }}
 
+            ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
+
   send-tweet:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
           python-version: "3.10"
       - name: Install dependencies
@@ -56,7 +63,7 @@ jobs:
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@v0.1.0
+      - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/.gitpod.yml b/.gitpod.yml
index 363d5b1d..105a1821 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -10,13 +10,11 @@ tasks:
 
 vscode:
   extensions: # based on nf-core.nf-core-extensionpack
-    - codezombiech.gitignore # Language support for .gitignore files
-    # - cssho.vscode-svgviewer                 # SVG viewer
     - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code
-    - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
     - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
     - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
     - mechatroner.rainbow-csv # Highlight columns in csv files in different colors
-    # - nextflow.nextflow                      # Nextflow syntax highlighting
+    # - nextflow.nextflow                    # Nextflow syntax highlighting
     - oderwat.indent-rainbow # Highlight indentation level
     - streetsidesoftware.code-spell-checker # Spelling checker for source code
+    - charliermarsh.ruff # Code linter Ruff
diff --git a/.nf-core.yml b/.nf-core.yml
index 98319d1b..71f34ac4 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,23 +1,24 @@
 lint:
   files_exist:
-  - CODE_OF_CONDUCT.md
-  - assets/nf-core-assemblyqc_logo_light.png
-  - docs/images/nf-core-assemblyqc_logo_light.png
-  - docs/images/nf-core-assemblyqc_logo_dark.png
-  - .github/ISSUE_TEMPLATE/config.yml
-  - .github/workflows/awstest.yml
-  - .github/workflows/awsfulltest.yml
+    - CODE_OF_CONDUCT.md
+    - assets/nf-core-assemblyqc_logo_light.png
+    - docs/images/nf-core-assemblyqc_logo_light.png
+    - docs/images/nf-core-assemblyqc_logo_dark.png
+    - .github/ISSUE_TEMPLATE/config.yml
+    - .github/workflows/awstest.yml
+    - .github/workflows/awsfulltest.yml
   files_unchanged:
-  - CODE_OF_CONDUCT.md
-  - assets/nf-core-assemblyqc_logo_light.png
-  - docs/images/nf-core-assemblyqc_logo_light.png
-  - docs/images/nf-core-assemblyqc_logo_dark.png
-  - .github/ISSUE_TEMPLATE/bug_report.yml
+    - CODE_OF_CONDUCT.md
+    - assets/nf-core-assemblyqc_logo_light.png
+    - docs/images/nf-core-assemblyqc_logo_light.png
+    - docs/images/nf-core-assemblyqc_logo_dark.png
+    - .github/ISSUE_TEMPLATE/bug_report.yml
   multiqc_config:
-  - report_comment
+    - report_comment
   nextflow_config:
-  - manifest.name
-  - manifest.homePage
+    - manifest.name
+    - manifest.homePage
+nf_core_version: 2.13.2.dev0
 repository_type: pipeline
 template:
   prefix: plant-food-research-open
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 984321ff..4dc0f1dc 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -1,8 +1,11 @@
 repos:
   - repo: https://github.com/pre-commit/mirrors-prettier
-    rev: "v3.1.1"
+    rev: "v3.1.0"
     hooks:
       - id: prettier
+        additional_dependencies:
+          - prettier@3.2.5
+
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
     rev: "2.7.3"
     hooks:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4aed6c94..59ee0da8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v1.4dev - [date]
+## v2.0.0 - [date]
 
 Initial release of plant-food-research-open/assemblyqc, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/LICENSE b/LICENSE
index bee1bb59..96e3eb88 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Usman Rashid
+Copyright (c) Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal
diff --git a/README.md b/README.md
index 92b64574..5ea7ea72 100644
--- a/README.md
+++ b/README.md
@@ -1,11 +1,12 @@
-[![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20CI/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+CI%22)
-[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+[![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/ci.yml/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/ci.yml)
+[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/linting.yml/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/linting.yml)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
-[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
+[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
 
 ## Introduction
 
@@ -62,7 +63,7 @@ nextflow run plant-food-research-open/assemblyqc \
 
 ## Credits
 
-plant-food-research-open/assemblyqc was originally written by Usman Rashid.
+plant-food-research-open/assemblyqc was originally written by Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
diff --git a/assets/email_template.html b/assets/email_template.html
index fa1b2da2..5a56215f 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -4,7 +4,7 @@
   <meta http-equiv="X-UA-Compatible" content="IE=edge">
   <meta name="viewport" content="width=device-width, initial-scale=1">
 
-  <meta name="description" content="plant-food-research-open/assemblyqc: AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.">
+  <meta name="description" content="plant-food-research-open/assemblyqc: A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.">
   <title>plant-food-research-open/assemblyqc Pipeline Report</title>
 </head>
 <body>
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 6d8df9b7..759adecd 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,6 +1,8 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/tree/dev" target="_blank">plant-food-research-open/assemblyqc</a>
+  
+  This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/releases/tag/2.0.0" target="_blank">plant-food-research-open/assemblyqc</a>
   analysis pipeline.
+  
 report_section_order:
   "plant-food-research-open-assemblyqc-methods-description":
     order: -1000
@@ -10,3 +12,5 @@ report_section_order:
     order: -1002
 
 export_plots: true
+
+disable_version_detection: true
diff --git a/assets/schema_input.json b/assets/schema_input.json
index cff17406..2a9a79ca 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -10,25 +10,22 @@
             "sample": {
                 "type": "string",
                 "pattern": "^\\S+$",
-                "errorMessage": "Sample name must be provided and cannot contain spaces"
+                "errorMessage": "Sample name must be provided and cannot contain spaces",
+                "meta": ["id"]
             },
             "fastq_1": {
                 "type": "string",
+                "format": "file-path",
+                "exists": true,
                 "pattern": "^\\S+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
-                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'",
-                "anyOf": [
-                    {
-                        "type": "string",
-                        "pattern": "^\\S+\\.f(ast)?q\\.gz$"
-                    },
-                    {
-                        "type": "string",
-                        "maxLength": 0
-                    }
-                ]
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             }
         },
         "required": ["sample", "fastq_1"]
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
deleted file mode 100755
index 4a758fe0..00000000
--- a/bin/check_samplesheet.py
+++ /dev/null
@@ -1,259 +0,0 @@
-#!/usr/bin/env python
-
-
-"""Provide a command line tool to validate and transform tabular samplesheets."""
-
-
-import argparse
-import csv
-import logging
-import sys
-from collections import Counter
-from pathlib import Path
-
-logger = logging.getLogger()
-
-
-class RowChecker:
-    """
-    Define a service that can validate and transform each given row.
-
-    Attributes:
-        modified (list): A list of dicts, where each dict corresponds to a previously
-            validated and transformed row. The order of rows is maintained.
-
-    """
-
-    VALID_FORMATS = (
-        ".fq.gz",
-        ".fastq.gz",
-    )
-
-    def __init__(
-        self,
-        sample_col="sample",
-        first_col="fastq_1",
-        second_col="fastq_2",
-        single_col="single_end",
-        **kwargs,
-    ):
-        """
-        Initialize the row checker with the expected column names.
-
-        Args:
-            sample_col (str): The name of the column that contains the sample name
-                (default "sample").
-            first_col (str): The name of the column that contains the first (or only)
-                FASTQ file path (default "fastq_1").
-            second_col (str): The name of the column that contains the second (if any)
-                FASTQ file path (default "fastq_2").
-            single_col (str): The name of the new column that will be inserted and
-                records whether the sample contains single- or paired-end sequencing
-                reads (default "single_end").
-
-        """
-        super().__init__(**kwargs)
-        self._sample_col = sample_col
-        self._first_col = first_col
-        self._second_col = second_col
-        self._single_col = single_col
-        self._seen = set()
-        self.modified = []
-
-    def validate_and_transform(self, row):
-        """
-        Perform all validations on the given row and insert the read pairing status.
-
-        Args:
-            row (dict): A mapping from column headers (keys) to elements of that row
-                (values).
-
-        """
-        self._validate_sample(row)
-        self._validate_first(row)
-        self._validate_second(row)
-        self._validate_pair(row)
-        self._seen.add((row[self._sample_col], row[self._first_col]))
-        self.modified.append(row)
-
-    def _validate_sample(self, row):
-        """Assert that the sample name exists and convert spaces to underscores."""
-        if len(row[self._sample_col]) <= 0:
-            raise AssertionError("Sample input is required.")
-        # Sanitize samples slightly.
-        row[self._sample_col] = row[self._sample_col].replace(" ", "_")
-
-    def _validate_first(self, row):
-        """Assert that the first FASTQ entry is non-empty and has the right format."""
-        if len(row[self._first_col]) <= 0:
-            raise AssertionError("At least the first FASTQ file is required.")
-        self._validate_fastq_format(row[self._first_col])
-
-    def _validate_second(self, row):
-        """Assert that the second FASTQ entry has the right format if it exists."""
-        if len(row[self._second_col]) > 0:
-            self._validate_fastq_format(row[self._second_col])
-
-    def _validate_pair(self, row):
-        """Assert that read pairs have the same file extension. Report pair status."""
-        if row[self._first_col] and row[self._second_col]:
-            row[self._single_col] = False
-            first_col_suffix = Path(row[self._first_col]).suffixes[-2:]
-            second_col_suffix = Path(row[self._second_col]).suffixes[-2:]
-            if first_col_suffix != second_col_suffix:
-                raise AssertionError("FASTQ pairs must have the same file extensions.")
-        else:
-            row[self._single_col] = True
-
-    def _validate_fastq_format(self, filename):
-        """Assert that a given filename has one of the expected FASTQ extensions."""
-        if not any(filename.endswith(extension) for extension in self.VALID_FORMATS):
-            raise AssertionError(
-                f"The FASTQ file has an unrecognized extension: {filename}\n"
-                f"It should be one of: {', '.join(self.VALID_FORMATS)}"
-            )
-
-    def validate_unique_samples(self):
-        """
-        Assert that the combination of sample name and FASTQ filename is unique.
-
-        In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the
-        number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment.
-
-        """
-        if len(self._seen) != len(self.modified):
-            raise AssertionError("The pair of sample name and FASTQ must be unique.")
-        seen = Counter()
-        for row in self.modified:
-            sample = row[self._sample_col]
-            seen[sample] += 1
-            row[self._sample_col] = f"{sample}_T{seen[sample]}"
-
-
-def read_head(handle, num_lines=10):
-    """Read the specified number of lines from the current position in the file."""
-    lines = []
-    for idx, line in enumerate(handle):
-        if idx == num_lines:
-            break
-        lines.append(line)
-    return "".join(lines)
-
-
-def sniff_format(handle):
-    """
-    Detect the tabular format.
-
-    Args:
-        handle (text file): A handle to a `text file`_ object. The read position is
-        expected to be at the beginning (index 0).
-
-    Returns:
-        csv.Dialect: The detected tabular format.
-
-    .. _text file:
-        https://docs.python.org/3/glossary.html#term-text-file
-
-    """
-    peek = read_head(handle)
-    handle.seek(0)
-    sniffer = csv.Sniffer()
-    dialect = sniffer.sniff(peek)
-    return dialect
-
-
-def check_samplesheet(file_in, file_out):
-    """
-    Check that the tabular samplesheet has the structure expected by nf-core pipelines.
-
-    Validate the general shape of the table, expected columns, and each row. Also add
-    an additional column which records whether one or two FASTQ reads were found.
-
-    Args:
-        file_in (pathlib.Path): The given tabular samplesheet. The format can be either
-            CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``.
-        file_out (pathlib.Path): Where the validated and transformed samplesheet should
-            be created; always in CSV format.
-
-    Example:
-        This function checks that the samplesheet follows the following structure,
-        see also the `viral recon samplesheet`_::
-
-            sample,fastq_1,fastq_2
-            SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
-            SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
-            SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
-
-    .. _viral recon samplesheet:
-        https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
-
-    """
-    required_columns = {"sample", "fastq_1", "fastq_2"}
-    # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`.
-    with file_in.open(newline="") as in_handle:
-        reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle))
-        # Validate the existence of the expected header columns.
-        if not required_columns.issubset(reader.fieldnames):
-            req_cols = ", ".join(required_columns)
-            logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.")
-            sys.exit(1)
-        # Validate each row.
-        checker = RowChecker()
-        for i, row in enumerate(reader):
-            try:
-                checker.validate_and_transform(row)
-            except AssertionError as error:
-                logger.critical(f"{str(error)} On line {i + 2}.")
-                sys.exit(1)
-        checker.validate_unique_samples()
-    header = list(reader.fieldnames)
-    header.insert(1, "single_end")
-    # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`.
-    with file_out.open(mode="w", newline="") as out_handle:
-        writer = csv.DictWriter(out_handle, header, delimiter=",")
-        writer.writeheader()
-        for row in checker.modified:
-            writer.writerow(row)
-
-
-def parse_args(argv=None):
-    """Define and immediately parse command line arguments."""
-    parser = argparse.ArgumentParser(
-        description="Validate and transform a tabular samplesheet.",
-        epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv",
-    )
-    parser.add_argument(
-        "file_in",
-        metavar="FILE_IN",
-        type=Path,
-        help="Tabular input samplesheet in CSV or TSV format.",
-    )
-    parser.add_argument(
-        "file_out",
-        metavar="FILE_OUT",
-        type=Path,
-        help="Transformed output samplesheet in CSV format.",
-    )
-    parser.add_argument(
-        "-l",
-        "--log-level",
-        help="The desired log level (default WARNING).",
-        choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"),
-        default="WARNING",
-    )
-    return parser.parse_args(argv)
-
-
-def main(argv=None):
-    """Coordinate argument parsing and program execution."""
-    args = parse_args(argv)
-    logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s")
-    if not args.file_in.is_file():
-        logger.error(f"The given input file {args.file_in} was not found!")
-        sys.exit(2)
-    args.file_out.parent.mkdir(parents=True, exist_ok=True)
-    check_samplesheet(args.file_in, args.file_out)
-
-
-if __name__ == "__main__":
-    sys.exit(main())
diff --git a/conf/base.config b/conf/base.config
index fad3aa91..faaec489 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -59,7 +59,4 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
-    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
-        cache = false
-    }
 }
diff --git a/conf/modules.config b/conf/modules.config
index d91c6aba..d203d2b6 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,26 +18,10 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
-    withName: SAMPLESHEET_CHECK {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: FASTQC {
         ext.args = '--quiet'
     }
 
-    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            pattern: '*_versions.yml'
-        ]
-    }
-
     withName: 'MULTIQC' {
         ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
deleted file mode 100755
index 4ce590c0..00000000
--- a/lib/NfcoreTemplate.groovy
+++ /dev/null
@@ -1,351 +0,0 @@
-//
-// This file holds several functions used within the nf-core pipeline template.
-//
-
-import org.yaml.snakeyaml.Yaml
-import groovy.json.JsonOutput
-import nextflow.extension.FilesEx
-
-class NfcoreTemplate {
-
-    //
-    // Check AWS Batch related parameters have been specified correctly
-    //
-    public static void awsBatch(workflow, params) {
-        if (workflow.profile.contains('awsbatch')) {
-            // Check params.awsqueue and params.awsregion have been set if running on AWSBatch
-            assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
-            // Check outdir paths to be S3 buckets if running on AWSBatch
-            assert params.outdir.startsWith('s3:')       : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
-        }
-    }
-
-    //
-    //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
-    //
-    public static void checkConfigProvided(workflow, log) {
-        if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
-            log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
-                    "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
-                    "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
-                    "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
-                    "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
-                    "Please refer to the quick start section and usage docs for the pipeline.\n "
-        }
-    }
-
-    //
-    // Generate version string
-    //
-    public static String version(workflow) {
-        String version_string = ""
-
-        if (workflow.manifest.version) {
-            def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
-            version_string += "${prefix_v}${workflow.manifest.version}"
-        }
-
-        if (workflow.commitId) {
-            def git_shortsha = workflow.commitId.substring(0, 7)
-            version_string += "-g${git_shortsha}"
-        }
-
-        return version_string
-    }
-
-    //
-    // Construct and send completion email
-    //
-    public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) {
-
-        // Set up the e-mail variables
-        def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
-        if (!workflow.success) {
-            subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
-        }
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['Date Started']              = workflow.start
-        misc_fields['Date Completed']            = workflow.complete
-        misc_fields['Pipeline script file path'] = workflow.scriptFile
-        misc_fields['Pipeline script hash ID']   = workflow.scriptId
-        if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
-        if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
-        if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
-        misc_fields['Nextflow Version']           = workflow.nextflow.version
-        misc_fields['Nextflow Build']             = workflow.nextflow.build
-        misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
-
-        def email_fields = [:]
-        email_fields['version']      = NfcoreTemplate.version(workflow)
-        email_fields['runName']      = workflow.runName
-        email_fields['success']      = workflow.success
-        email_fields['dateComplete'] = workflow.complete
-        email_fields['duration']     = workflow.duration
-        email_fields['exitStatus']   = workflow.exitStatus
-        email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        email_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        email_fields['commandLine']  = workflow.commandLine
-        email_fields['projectDir']   = workflow.projectDir
-        email_fields['summary']      = summary << misc_fields
-
-        // On success try attach the multiqc report
-        def mqc_report = null
-        try {
-            if (workflow.success) {
-                mqc_report = multiqc_report.getVal()
-                if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
-                    if (mqc_report.size() > 1) {
-                        log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
-                    }
-                    mqc_report = mqc_report[0]
-                }
-            }
-        } catch (all) {
-            if (multiqc_report) {
-                log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
-            }
-        }
-
-        // Check if we are only sending emails on failure
-        def email_address = params.email
-        if (!params.email && params.email_on_fail && !workflow.success) {
-            email_address = params.email_on_fail
-        }
-
-        // Render the TXT template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        def tf           = new File("$projectDir/assets/email_template.txt")
-        def txt_template = engine.createTemplate(tf).make(email_fields)
-        def email_txt    = txt_template.toString()
-
-        // Render the HTML template
-        def hf            = new File("$projectDir/assets/email_template.html")
-        def html_template = engine.createTemplate(hf).make(email_fields)
-        def email_html    = html_template.toString()
-
-        // Render the sendmail template
-        def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
-        def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
-        def sf                     = new File("$projectDir/assets/sendmail_template.txt")
-        def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
-        def sendmail_html          = sendmail_template.toString()
-
-        // Send the HTML e-mail
-        Map colors = logColours(params.monochrome_logs)
-        if (email_address) {
-            try {
-                if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
-                // Try to send HTML e-mail using sendmail
-                def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
-                sendmail_tf.withWriter { w -> w << sendmail_html }
-                [ 'sendmail', '-t' ].execute() << sendmail_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
-            } catch (all) {
-                // Catch failures and try with plaintext
-                def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-                if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
-                    mail_cmd += [ '-A', mqc_report ]
-                }
-                mail_cmd.execute() << email_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
-            }
-        }
-
-        // Write summary e-mail HTML to a file
-        def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
-        output_hf.withWriter { w -> w << email_html }
-        FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html");
-        output_hf.delete()
-
-        // Write summary e-mail TXT to a file
-        def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
-        output_tf.withWriter { w -> w << email_txt }
-        FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt");
-        output_tf.delete()
-    }
-
-    //
-    // Construct and send a notification to a web server as JSON
-    // e.g. Microsoft Teams and Slack
-    //
-    public static void IM_notification(workflow, params, summary_params, projectDir, log) {
-        def hook_url = params.hook_url
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['start']                                = workflow.start
-        misc_fields['complete']                             = workflow.complete
-        misc_fields['scriptfile']                           = workflow.scriptFile
-        misc_fields['scriptid']                             = workflow.scriptId
-        if (workflow.repository) misc_fields['repository']  = workflow.repository
-        if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
-        if (workflow.revision)   misc_fields['revision']    = workflow.revision
-        misc_fields['nxf_version']                          = workflow.nextflow.version
-        misc_fields['nxf_build']                            = workflow.nextflow.build
-        misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
-
-        def msg_fields = [:]
-        msg_fields['version']      = NfcoreTemplate.version(workflow)
-        msg_fields['runName']      = workflow.runName
-        msg_fields['success']      = workflow.success
-        msg_fields['dateComplete'] = workflow.complete
-        msg_fields['duration']     = workflow.duration
-        msg_fields['exitStatus']   = workflow.exitStatus
-        msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
-        msg_fields['projectDir']   = workflow.projectDir
-        msg_fields['summary']      = summary << misc_fields
-
-        // Render the JSON template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        // Different JSON depending on the service provider
-        // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
-        def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
-        def hf            = new File("$projectDir/assets/${json_path}")
-        def json_template = engine.createTemplate(hf).make(msg_fields)
-        def json_message  = json_template.toString()
-
-        // POST
-        def post = new URL(hook_url).openConnection();
-        post.setRequestMethod("POST")
-        post.setDoOutput(true)
-        post.setRequestProperty("Content-Type", "application/json")
-        post.getOutputStream().write(json_message.getBytes("UTF-8"));
-        def postRC = post.getResponseCode();
-        if (! postRC.equals(200)) {
-            log.warn(post.getErrorStream().getText());
-        }
-    }
-
-    //
-    // Dump pipeline parameters in a json file
-    //
-    public static void dump_parameters(workflow, params) {
-        def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-        def filename   = "params_${timestamp}.json"
-        def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
-        def jsonStr    = JsonOutput.toJson(params)
-        temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
-
-        FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json")
-        temp_pf.delete()
-    }
-
-    //
-    // Print pipeline summary on completion
-    //
-    public static void summary(workflow, params, log) {
-        Map colors = logColours(params.monochrome_logs)
-        if (workflow.success) {
-            if (workflow.stats.ignoredCount == 0) {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
-            } else {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
-            }
-        } else {
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
-        }
-    }
-
-    //
-    // ANSII Colours used for terminal logging
-    //
-    public static Map logColours(Boolean monochrome_logs) {
-        Map colorcodes = [:]
-
-        // Reset / Meta
-        colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
-        colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
-        colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
-        colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
-        colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
-        colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
-        colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
-
-        // Regular Colors
-        colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
-        colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
-        colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
-        colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
-        colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
-        colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
-        colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
-        colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
-
-        // Bold
-        colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
-        colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
-        colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
-        colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
-        colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
-        colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
-        colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
-        colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
-
-        // Underline
-        colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
-        colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
-        colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
-        colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
-        colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
-        colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
-        colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
-        colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
-
-        // High Intensity
-        colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
-        colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
-        colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
-        colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
-        colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
-        colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
-        colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
-        colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
-
-        // Bold High Intensity
-        colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
-        colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
-        colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
-        colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
-        colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
-        colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
-        colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
-        colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
-
-        return colorcodes
-    }
-
-    //
-    // Does what is says on the tin
-    //
-    public static String dashedLine(monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-    }
-
-    //
-    // nf-core logo
-    //
-    public static String logo(workflow, monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        String workflow_version = NfcoreTemplate.version(workflow)
-        String.format(
-            """\n
-            ${dashedLine(monochrome_logs)}
-            ${colors.purple}  ${workflow.manifest.name} ${workflow_version}${colors.reset}
-            ${dashedLine(monochrome_logs)}
-            """.stripIndent()
-        )
-    }
-}
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
deleted file mode 100644
index 8d030f4e..00000000
--- a/lib/Utils.groovy
+++ /dev/null
@@ -1,47 +0,0 @@
-//
-// This file holds several Groovy functions that could be useful for any Nextflow pipeline
-//
-
-import org.yaml.snakeyaml.Yaml
-
-class Utils {
-
-    //
-    // When running with -profile conda, warn if channels have not been set-up appropriately
-    //
-    public static void checkCondaChannels(log) {
-        Yaml parser = new Yaml()
-        def channels = []
-        try {
-            def config = parser.load("conda config --show channels".execute().text)
-            channels = config.channels
-        } catch(NullPointerException | IOException e) {
-            log.warn "Could not verify conda channel configuration."
-            return
-        }
-
-        // Check that all channels are present
-        // This channel list is ordered by required channel priority.
-        def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
-        def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
-
-        // Check that they are in the right order
-        def channel_priority_violation = false
-        def n = required_channels_in_order.size()
-        for (int i = 0; i < n - 1; i++) {
-            channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
-        }
-
-        if (channels_missing | channel_priority_violation) {
-            log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  There is a problem with your Conda configuration!\n\n" +
-                "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
-                "  Please refer to https://bioconda.github.io/\n" +
-                "  The observed channel order is \n" +
-                "  ${channels}\n" +
-                "  but the following channel order is required:\n" +
-                "  ${required_channels_in_order}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-        }
-    }
-}
diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy
deleted file mode 100755
index 8be44f94..00000000
--- a/lib/WorkflowAssemblyqc.groovy
+++ /dev/null
@@ -1,122 +0,0 @@
-//
-// This file holds several functions specific to the workflow/assemblyqc.nf in the plant-food-research-open/assemblyqc pipeline
-//
-
-import nextflow.Nextflow
-import groovy.text.SimpleTemplateEngine
-
-class WorkflowAssemblyqc {
-
-    //
-    // Check and validate parameters
-    //
-    public static void initialise(params, log) {
-
-        genomeExistsError(params, log)
-
-
-        if (!params.fasta) {
-            Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
-        }
-    }
-
-    //
-    // Get workflow summary for MultiQC
-    //
-    public static String paramsSummaryMultiqc(workflow, summary) {
-        String summary_section = ''
-        for (group in summary.keySet()) {
-            def group_params = summary.get(group)  // This gets the parameters of that particular group
-            if (group_params) {
-                summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
-                summary_section += "    <dl class=\"dl-horizontal\">\n"
-                for (param in group_params.keySet()) {
-                    summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
-                }
-                summary_section += "    </dl>\n"
-            }
-        }
-
-        String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
-        yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
-        yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
-        yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
-        yaml_file_text        += "plot_type: 'html'\n"
-        yaml_file_text        += "data: |\n"
-        yaml_file_text        += "${summary_section}"
-        return yaml_file_text
-    }
-
-    //
-    // Generate methods description for MultiQC
-    //
-
-    public static String toolCitationText(params) {
-
-        // TODO nf-core: Optionally add in-text citation tools to this list.
-        // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
-        // Uncomment function in methodsDescriptionText to render in MultiQC report
-        def citation_text = [
-                "Tools used in the workflow included:",
-                "FastQC (Andrews 2010),",
-                "MultiQC (Ewels et al. 2016)",
-                "."
-            ].join(' ').trim()
-
-        return citation_text
-    }
-
-    public static String toolBibliographyText(params) {
-
-        // TODO Optionally add bibliographic entries to this list.
-        // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "<li>Author (2023) Pub name, Journal, DOI</li>" : "",
-        // Uncomment function in methodsDescriptionText to render in MultiQC report
-        def reference_text = [
-                "<li>Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).</li>",
-                "<li>Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354</li>"
-            ].join(' ').trim()
-
-        return reference_text
-    }
-
-    public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) {
-        // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
-        def meta = [:]
-        meta.workflow = run_workflow.toMap()
-        meta["manifest_map"] = run_workflow.manifest.toMap()
-
-        // Pipeline DOI
-        meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
-        meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
-
-        // Tool references
-        meta["tool_citations"] = ""
-        meta["tool_bibliography"] = ""
-
-        // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
-        //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".")
-        //meta["tool_bibliography"] = toolBibliographyText(params)
-
-
-        def methods_text = mqc_methods_yaml.text
-
-        def engine =  new SimpleTemplateEngine()
-        def description_html = engine.createTemplate(methods_text).make(meta)
-
-        return description_html
-    }
-
-    //
-    // Exit pipeline if incorrect --genome key provided
-    //
-    private static void genomeExistsError(params, log) {
-        if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-            def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
-                "  Currently, the available genome keys are:\n" +
-                "  ${params.genomes.keySet().join(", ")}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-            Nextflow.error(error_string)
-        }
-    }
-}
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
deleted file mode 100755
index b7f82557..00000000
--- a/lib/WorkflowMain.groovy
+++ /dev/null
@@ -1,63 +0,0 @@
-//
-// This file holds several functions specific to the main.nf workflow in the plant-food-research-open/assemblyqc pipeline
-//
-
-import nextflow.Nextflow
-
-class WorkflowMain {
-
-    //
-    // Citation string for pipeline
-    //
-    public static String citation(workflow) {
-        return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-            // TODO nf-core: Add Zenodo DOI for pipeline after first release
-            //"* The pipeline\n" +
-            //"  https://doi.org/10.5281/zenodo.XXXXXXX\n\n" +
-            "* The nf-core framework\n" +
-            "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
-            "* Software dependencies\n" +
-            "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
-    }
-
-
-    //
-    // Validate parameters and print summary to screen
-    //
-    public static void initialise(workflow, params, log) {
-
-        // Print workflow version and exit on --version
-        if (params.version) {
-            String workflow_version = NfcoreTemplate.version(workflow)
-            log.info "${workflow.manifest.name} ${workflow_version}"
-            System.exit(0)
-        }
-
-        // Check that a -profile or Nextflow config has been provided to run the pipeline
-        NfcoreTemplate.checkConfigProvided(workflow, log)
-
-        // Check that conda channels are set-up correctly
-        if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-            Utils.checkCondaChannels(log)
-        }
-
-        // Check AWS batch settings
-        NfcoreTemplate.awsBatch(workflow, params)
-
-        // Check input has been provided
-        if (!params.input) {
-            Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'")
-        }
-    }
-    //
-    // Get attribute from genome config file e.g. fasta
-    //
-    public static Object getGenomeAttribute(params, attribute) {
-        if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
-            if (params.genomes[ params.genome ].containsKey(attribute)) {
-                return params.genomes[ params.genome ][ attribute ]
-            }
-        }
-        return null
-    }
-}
diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar
deleted file mode 100644
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GIT binary patch
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diff --git a/main.nf b/main.nf
index 42a3a13f..e417dccf 100644
--- a/main.nf
+++ b/main.nf
@@ -11,66 +11,96 @@ nextflow.enable.dsl = 2
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    GENOME PARAMETER VALUES
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-// TODO nf-core: Remove this line if you don't need a FASTA file
-//   This is an example of how to use getGenomeAttribute() to fetch parameters
-//   from igenomes.config using `--genome`
-params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
+include { ASSEMBLYQC  } from './workflows/assemblyqc'
+include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline'
+include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline'
+
+include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    VALIDATE & PRINT PARAMETER SUMMARY
+    GENOME PARAMETER VALUES
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { validateParameters; paramsHelp } from 'plugin/nf-validation'
-
-// Print help message if needed
-if (params.help) {
-    def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-    def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-    def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
-    log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs)
-    System.exit(0)
-}
-
-// Validate input parameters
-if (params.validate_params) {
-    validateParameters()
-}
-
-WorkflowMain.initialise(workflow, params, log)
+// TODO nf-core: Remove this line if you don't need a FASTA file
+//   This is an example of how to use getGenomeAttribute() to fetch parameters
+//   from igenomes.config using `--genome`
+params.fasta = getGenomeAttribute('fasta')
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    NAMED WORKFLOW FOR PIPELINE
+    NAMED WORKFLOWS FOR PIPELINE
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { ASSEMBLYQC } from './workflows/assemblyqc'
-
 //
-// WORKFLOW: Run main plant-food-research-open/assemblyqc analysis pipeline
+// WORKFLOW: Run main analysis pipeline depending on type of input
 //
 workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
-    ASSEMBLYQC ()
-}
 
+    take:
+    samplesheet // channel: samplesheet read in from --input
+
+    main:
+
+    //
+    // WORKFLOW: Run pipeline
+    //
+    ASSEMBLYQC (
+        samplesheet
+    )
+
+    emit:
+    multiqc_report = ASSEMBLYQC.out.multiqc_report // channel: /path/to/multiqc_report.html
+
+}
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    RUN ALL WORKFLOWS
+    RUN MAIN WORKFLOW
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-//
-// WORKFLOW: Execute a single named workflow for the pipeline
-// See: https://github.com/nf-core/rnaseq/issues/619
-//
 workflow {
-    PLANTFOODRESEARCHOPEN_ASSEMBLYQC ()
+
+    main:
+
+    //
+    // SUBWORKFLOW: Run initialisation tasks
+    //
+    PIPELINE_INITIALISATION (
+        params.version,
+        params.help,
+        params.validate_params,
+        params.monochrome_logs,
+        args,
+        params.outdir,
+        params.input
+    )
+
+    //
+    // WORKFLOW: Run main workflow
+    //
+    PLANTFOODRESEARCHOPEN_ASSEMBLYQC (
+        PIPELINE_INITIALISATION.out.samplesheet
+    )
+
+    //
+    // SUBWORKFLOW: Run completion tasks
+    //
+    PIPELINE_COMPLETION (
+        params.email,
+        params.email_on_fail,
+        params.plaintext_email,
+        params.outdir,
+        params.monochrome_logs,
+        params.hook_url,
+        PLANTFOODRESEARCHOPEN_ASSEMBLYQC.out.multiqc_report
+    )
 }
 
 /*
diff --git a/modules.json b/modules.json
index f3c7519d..1d7adb5b 100644
--- a/modules.json
+++ b/modules.json
@@ -5,22 +5,36 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
-                    "custom/dumpsoftwareversions": {
-                        "branch": "master",
-                        "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e",
-                        "installed_by": ["modules"]
-                    },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
+                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "4ab13872435962dadc239979554d13709e20bf29",
+                        "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
                         "installed_by": ["modules"]
                     }
                 }
+            },
+            "subworkflows": {
+                "nf-core": {
+                    "utils_nextflow_pipeline": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": ["subworkflows"]
+                    },
+                    "utils_nfcore_pipeline": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": ["subworkflows"]
+                    },
+                    "utils_nfvalidation_plugin": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": ["subworkflows"]
+                    }
+                }
             }
         }
     }
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
deleted file mode 100644
index 7a776dbd..00000000
--- a/modules/local/samplesheet_check.nf
+++ /dev/null
@@ -1,31 +0,0 @@
-process SAMPLESHEET_CHECK {
-    tag "$samplesheet"
-    label 'process_single'
-
-    conda "conda-forge::python=3.8.3"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'biocontainers/python:3.8.3' }"
-
-    input:
-    path samplesheet
-
-    output:
-    path '*.csv'       , emit: csv
-    path "versions.yml", emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script: // This script is bundled with the pipeline, in plant-food-research-open/assemblyqc/bin/
-    """
-    check_samplesheet.py \\
-        $samplesheet \\
-        samplesheet.valid.csv
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed 's/Python //g')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
deleted file mode 100644
index f0c63f69..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: custom_dumpsoftwareversions
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::multiqc=1.17
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
deleted file mode 100644
index 7685b33c..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ /dev/null
@@ -1,24 +0,0 @@
-process CUSTOM_DUMPSOFTWAREVERSIONS {
-    label 'process_single'
-
-    // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.17--pyhdfd78af_0' }"
-
-    input:
-    path versions
-
-    output:
-    path "software_versions.yml"    , emit: yml
-    path "software_versions_mqc.yml", emit: mqc_yml
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    template 'dumpsoftwareversions.py'
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
deleted file mode 100644
index 5f15a5fd..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ /dev/null
@@ -1,37 +0,0 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
-name: custom_dumpsoftwareversions
-description: Custom module used to dump software versions within the nf-core pipeline template
-keywords:
-  - custom
-  - dump
-  - version
-tools:
-  - custom:
-      description: Custom module used to dump software versions within the nf-core pipeline template
-      homepage: https://github.com/nf-core/tools
-      documentation: https://github.com/nf-core/tools
-      licence: ["MIT"]
-input:
-  - versions:
-      type: file
-      description: YML file containing software versions
-      pattern: "*.yml"
-output:
-  - yml:
-      type: file
-      description: Standard YML file containing software versions
-      pattern: "software_versions.yml"
-  - mqc_yml:
-      type: file
-      description: MultiQC custom content YML file containing software versions
-      pattern: "software_versions_mqc.yml"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@grst"
-maintainers:
-  - "@drpatelh"
-  - "@grst"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
deleted file mode 100755
index e55b8d43..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ /dev/null
@@ -1,102 +0,0 @@
-#!/usr/bin/env python
-
-
-"""Provide functions to merge multiple versions.yml files."""
-
-
-import platform
-from textwrap import dedent
-
-import yaml
-
-
-def _make_versions_html(versions):
-    """Generate a tabular HTML output of all versions for MultiQC."""
-    html = [
-        dedent(
-            """\\
-            <style>
-            #nf-core-versions tbody:nth-child(even) {
-                background-color: #f2f2f2;
-            }
-            </style>
-            <table class="table" style="width:100%" id="nf-core-versions">
-                <thead>
-                    <tr>
-                        <th> Process Name </th>
-                        <th> Software </th>
-                        <th> Version  </th>
-                    </tr>
-                </thead>
-            """
-        )
-    ]
-    for process, tmp_versions in sorted(versions.items()):
-        html.append("<tbody>")
-        for i, (tool, version) in enumerate(sorted(tmp_versions.items())):
-            html.append(
-                dedent(
-                    f"""\\
-                    <tr>
-                        <td><samp>{process if (i == 0) else ''}</samp></td>
-                        <td><samp>{tool}</samp></td>
-                        <td><samp>{version}</samp></td>
-                    </tr>
-                    """
-                )
-            )
-        html.append("</tbody>")
-    html.append("</table>")
-    return "\\n".join(html)
-
-
-def main():
-    """Load all version files and generate merged output."""
-    versions_this_module = {}
-    versions_this_module["${task.process}"] = {
-        "python": platform.python_version(),
-        "yaml": yaml.__version__,
-    }
-
-    with open("$versions") as f:
-        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
-
-    # aggregate versions by the module name (derived from fully-qualified process name)
-    versions_by_module = {}
-    for process, process_versions in versions_by_process.items():
-        module = process.split(":")[-1]
-        try:
-            if versions_by_module[module] != process_versions:
-                raise AssertionError(
-                    "We assume that software versions are the same between all modules. "
-                    "If you see this error-message it means you discovered an edge-case "
-                    "and should open an issue in nf-core/tools. "
-                )
-        except KeyError:
-            versions_by_module[module] = process_versions
-
-    versions_by_module["Workflow"] = {
-        "Nextflow": "$workflow.nextflow.version",
-        "$workflow.manifest.name": "$workflow.manifest.version",
-    }
-
-    versions_mqc = {
-        "id": "software_versions",
-        "section_name": "${workflow.manifest.name} Software Versions",
-        "section_href": "https://github.com/${workflow.manifest.name}",
-        "plot_type": "html",
-        "description": "are collected at run time from the software output.",
-        "data": _make_versions_html(versions_by_module),
-    }
-
-    with open("software_versions.yml", "w") as f:
-        yaml.dump(versions_by_module, f, default_flow_style=False)
-    with open("software_versions_mqc.yml", "w") as f:
-        yaml.dump(versions_mqc, f, default_flow_style=False)
-
-    with open("versions.yml", "w") as f:
-        yaml.dump(versions_this_module, f, default_flow_style=False)
-
-
-if __name__ == "__main__":
-    main()
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
deleted file mode 100644
index eec1db10..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
+++ /dev/null
@@ -1,38 +0,0 @@
-nextflow_process {
-
-    name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS"
-    script "../main.nf"
-    process "CUSTOM_DUMPSOFTWAREVERSIONS"
-    tag "modules"
-    tag "modules_nfcore"
-    tag "custom"
-    tag "dumpsoftwareversions"
-    tag "custom/dumpsoftwareversions"
-
-    test("Should run without failures") {
-        when {
-            process {
-                """
-                def tool1_version = '''
-                TOOL1:
-                    tool1: 0.11.9
-                '''.stripIndent()
-
-                def tool2_version = '''
-                TOOL2:
-                    tool2: 1.9
-                '''.stripIndent()
-
-                input[0] = Channel.of(tool1_version, tool2_version).collectFile()
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
deleted file mode 100644
index 4274ed57..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
+++ /dev/null
@@ -1,27 +0,0 @@
-{
-    "Should run without failures": {
-        "content": [
-            {
-                "0": [
-                    "software_versions.yml:md5,1c851188476409cda5752ce971b20b58"
-                ],
-                "1": [
-                    "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d"
-                ],
-                "2": [
-                    "versions.yml:md5,3843ac526e762117eedf8825b40683df"
-                ],
-                "mqc_yml": [
-                    "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d"
-                ],
-                "versions": [
-                    "versions.yml:md5,3843ac526e762117eedf8825b40683df"
-                ],
-                "yml": [
-                    "software_versions.yml:md5,1c851188476409cda5752ce971b20b58"
-                ]
-            }
-        ],
-        "timestamp": "2023-11-03T14:43:22.157011"
-    }
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
deleted file mode 100644
index 405aa24a..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-custom/dumpsoftwareversions:
-  - modules/nf-core/custom/dumpsoftwareversions/**
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index b9e8f926..70edae4d 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -3,24 +3,20 @@ nextflow_process {
     name "Test Process FASTQC"
     script "../main.nf"
     process "FASTQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "fastqc"
 
-    test("Single-Read") {
+    test("sarscov2 single-end [fastq]") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id: 'test', single_end:true ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 """
             }
         }
@@ -28,82 +24,189 @@ nextflow_process {
         then {
             assertAll (
             { assert process.success },
+
             // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
             // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
             // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
-            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert snapshot(process.out.versions).match("versions") },
-            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("fastqc_versions_single") }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("fastqc_versions_paired") }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") }
             )
         }
     }
-// TODO
-// //
-// // Test with paired-end data
-// //
-// workflow test_fastqc_paired_end {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 [
-//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-//                 ]
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with interleaved data
-// //
-// workflow test_fastqc_interleaved {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with bam data
-// //
-// workflow test_fastqc_bam {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with multiple samples
-// //
-// workflow test_fastqc_multiple {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 [
-//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true)
-//                 ]
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with custom prefix
-// //
-// workflow test_fastqc_custom_prefix {
-//     input = [
-//                 [ id:'mysample', single_end:true ], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
+
+    test("sarscov2 paired-end [bam]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("fastqc_versions_bam") }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+            { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+            { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("fastqc_versions_multiple") }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastq] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id: 'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
+                                process.out.zip.collect { file(it[1]).getName() } +
+                                process.out.versions ).match("fastqc_stub") }
+            )
+        }
+    }
+
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 636a32ce..86f7c311 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,10 +1,88 @@
 {
-    "versions": {
+    "fastqc_versions_interleaved": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2023-10-09T23:40:54+0000"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:07.293713"
+    },
+    "fastqc_stub": {
+        "content": [
+            [
+                "test.html",
+                "test.zip",
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:31:01.425198"
+    },
+    "fastqc_versions_multiple": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:55.797907"
+    },
+    "fastqc_versions_bam": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:26.795862"
+    },
+    "fastqc_versions_single": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:39:27.043675"
+    },
+    "fastqc_versions_paired": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:39:47.584191"
+    },
+    "fastqc_versions_custom_prefix": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:41:14.576531"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index bc0bdb5b..ca39fb67 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.18
+  - bioconda::multiqc=1.21
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 00cc48d2..47ac352f 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.18--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.21--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
@@ -43,7 +43,7 @@ process MULTIQC {
 
     stub:
     """
-    touch multiqc_data
+    mkdir multiqc_data
     touch multiqc_plots
     touch multiqc_report.html
 
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index f1aa660e..45a9bc35 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,4 +1,3 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: multiqc
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index c2dad217..f1c4242e 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -3,19 +3,17 @@ nextflow_process {
     name "Test Process MULTIQC"
     script "../main.nf"
     process "MULTIQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "multiqc"
 
-    test("MULTIQC: FASTQC") {
+    test("sarscov2 single-end [fastqc]") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = []
                 input[2] = []
                 input[3] = []
@@ -26,23 +24,20 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert path(process.out.report.get(0)).exists() },
-                { assert path(process.out.data.get(0)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("multiqc_versions_single") }
             )
         }
 
     }
 
-    test("MULTIQC: FASTQC and a config file") {
+    test("sarscov2 single-end [fastqc] [config]") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
                 input[2] = []
                 input[3] = []
@@ -53,9 +48,35 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert path(process.out.report.get(0)).exists() },
-                { assert path(process.out.data.get(0)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("multiqc_versions_config") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastqc] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.report.collect { file(it).getName() } +
+                                process.out.data.collect { file(it).getName() } +
+                                process.out.plots.collect { file(it).getName() } +
+                                process.out.versions ).match("multiqc_stub") }
             )
         }
 
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..bfebd802
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -0,0 +1,41 @@
+{
+    "multiqc_versions_single": {
+        "content": [
+            [
+                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-29T08:48:55.657331"
+    },
+    "multiqc_stub": {
+        "content": [
+            [
+                "multiqc_report.html",
+                "multiqc_data",
+                "multiqc_plots",
+                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-29T08:49:49.071937"
+    },
+    "multiqc_versions_config": {
+        "content": [
+            [
+                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-29T08:49:25.457567"
+    }
+}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 3db97ece..c89ce932 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -15,10 +15,7 @@ params {
     // References
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
-    igenomes_ignore            = false
-
-
-    // MultiQC options
+    igenomes_ignore            = false// MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     multiqc_logo               = null
@@ -44,7 +41,6 @@ params {
     config_profile_contact     = null
     config_profile_url         = null
 
-
     // Max resource options
     // Defaults only, expecting to be overwritten
     max_memory                 = '128.GB'
@@ -229,12 +225,12 @@ dag {
 
 manifest {
     name            = 'plant-food-research-open/assemblyqc'
-    author          = """Usman Rashid"""
+    author          = """Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy"""
     homePage        = 'https://github.com/plant-food-research-open/assemblyqc'
-    description     = """AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report."""
+    description     = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '1.4dev'
+    version         = '2.0.0'
     doi             = ''
 }
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 1a13c92b..dda3a0f5 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -2,7 +2,7 @@
     "$schema": "http://json-schema.org/draft-07/schema",
     "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/nextflow_schema.json",
     "title": "plant-food-research-open/assemblyqc pipeline parameters",
-    "description": "AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.",
+    "description": "A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.",
     "type": "object",
     "definitions": {
         "input_output_options": {
@@ -16,6 +16,7 @@
                     "type": "string",
                     "format": "file-path",
                     "exists": true,
+                    "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",
                     "description": "Path to comma-separated file containing information about the samples in the experiment.",
diff --git a/pyproject.toml b/pyproject.toml
deleted file mode 100644
index 984c0910..00000000
--- a/pyproject.toml
+++ /dev/null
@@ -1,13 +0,0 @@
-# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Ruff.
-# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
-[tool.ruff]
-line-length = 120
-target-version = "py38"
-select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
-cache-dir = "~/.cache/ruff"
-
-[tool.ruff.isort]
-known-first-party = ["nf_core"]
-
-[tool.ruff.per-file-ignores]
-"__init__.py" = ["E402", "F401"]
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
deleted file mode 100644
index 0aecf87f..00000000
--- a/subworkflows/local/input_check.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-//
-// Check input samplesheet and get read channels
-//
-
-include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check'
-
-workflow INPUT_CHECK {
-    take:
-    samplesheet // file: /path/to/samplesheet.csv
-
-    main:
-    SAMPLESHEET_CHECK ( samplesheet )
-        .csv
-        .splitCsv ( header:true, sep:',' )
-        .map { create_fastq_channel(it) }
-        .set { reads }
-
-    emit:
-    reads                                     // channel: [ val(meta), [ reads ] ]
-    versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ]
-}
-
-// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
-def create_fastq_channel(LinkedHashMap row) {
-    // create meta map
-    def meta = [:]
-    meta.id         = row.sample
-    meta.single_end = row.single_end.toBoolean()
-
-    // add path(s) of the fastq file(s) to the meta map
-    def fastq_meta = []
-    if (!file(row.fastq_1).exists()) {
-        exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
-    }
-    if (meta.single_end) {
-        fastq_meta = [ meta, [ file(row.fastq_1) ] ]
-    } else {
-        if (!file(row.fastq_2).exists()) {
-            exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
-        }
-        fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
-    }
-    return fastq_meta
-}
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
new file mode 100644
index 00000000..ac17938f
--- /dev/null
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -0,0 +1,255 @@
+//
+// Subworkflow with functionality specific to the plant-food-research-open/assemblyqc pipeline
+//
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin'
+include { paramsSummaryMap          } from 'plugin/nf-validation'
+include { fromSamplesheet           } from 'plugin/nf-validation'
+include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
+include { completionEmail           } from '../../nf-core/utils_nfcore_pipeline'
+include { completionSummary         } from '../../nf-core/utils_nfcore_pipeline'
+include { dashedLine                } from '../../nf-core/utils_nfcore_pipeline'
+include { nfCoreLogo                } from '../../nf-core/utils_nfcore_pipeline'
+include { imNotification            } from '../../nf-core/utils_nfcore_pipeline'
+include { UTILS_NFCORE_PIPELINE     } from '../../nf-core/utils_nfcore_pipeline'
+include { workflowCitation          } from '../../nf-core/utils_nfcore_pipeline'
+
+/*
+========================================================================================
+    SUBWORKFLOW TO INITIALISE PIPELINE
+========================================================================================
+*/
+
+workflow PIPELINE_INITIALISATION {
+
+    take:
+    version           // boolean: Display version and exit
+    help              // boolean: Display help text
+    validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
+    monochrome_logs   // boolean: Do not use coloured log outputs
+    nextflow_cli_args //   array: List of positional nextflow CLI args
+    outdir            //  string: The output directory where the results will be saved
+    input             //  string: Path to input samplesheet
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    //
+    // Print version and exit if required and dump pipeline parameters to JSON file
+    //
+    UTILS_NEXTFLOW_PIPELINE (
+        version,
+        true,
+        outdir,
+        workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1
+    )
+
+    //
+    // Validate parameters and generate parameter summary to stdout
+    //
+    pre_help_text = nfCoreLogo(monochrome_logs)
+    post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs)
+    def String workflow_command = "nextflow run ${workflow.manifest.name} -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
+    UTILS_NFVALIDATION_PLUGIN (
+        help,
+        workflow_command,
+        pre_help_text,
+        post_help_text,
+        validate_params,
+        "nextflow_schema.json"
+    )
+
+    //
+    // Check config provided to the pipeline
+    //
+    UTILS_NFCORE_PIPELINE (
+        nextflow_cli_args
+    )
+    //
+    // Custom validation for pipeline parameters
+    //
+    validateInputParameters()
+
+    //
+    // Create channel from input file provided through params.input
+    //
+    Channel
+        .fromSamplesheet("input")
+        .map {
+            meta, fastq_1, fastq_2 ->
+                if (!fastq_2) {
+                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+                } else {
+                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+                }
+        }
+        .groupTuple()
+        .map {
+            validateInputSamplesheet(it)
+        }
+        .map {
+            meta, fastqs ->
+                return [ meta, fastqs.flatten() ]
+        }
+        .set { ch_samplesheet }
+
+    emit:
+    samplesheet = ch_samplesheet
+    versions    = ch_versions
+}
+
+/*
+========================================================================================
+    SUBWORKFLOW FOR PIPELINE COMPLETION
+========================================================================================
+*/
+
+workflow PIPELINE_COMPLETION {
+
+    take:
+    email           //  string: email address
+    email_on_fail   //  string: email address sent on pipeline failure
+    plaintext_email // boolean: Send plain-text email instead of HTML
+    outdir          //    path: Path to output directory where results will be published
+    monochrome_logs // boolean: Disable ANSI colour codes in log output
+    hook_url        //  string: hook URL for notifications
+    multiqc_report  //  string: Path to MultiQC report
+
+    main:
+
+    summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+
+    //
+    // Completion email and summary
+    //
+    workflow.onComplete {
+        if (email || email_on_fail) {
+            completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList())
+        }
+
+        completionSummary(monochrome_logs)
+
+        if (hook_url) {
+            imNotification(summary_params, hook_url)
+        }
+    }
+
+    workflow.onError {
+        log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting"
+    }
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+//
+// Check and validate pipeline parameters
+//
+def validateInputParameters() {
+    genomeExistsError()
+}
+
+//
+// Validate channels from input samplesheet
+//
+def validateInputSamplesheet(input) {
+    def (metas, fastqs) = input[1..2]
+
+    // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end
+    def endedness_ok = metas.collect{ it.single_end }.unique().size == 1
+    if (!endedness_ok) {
+        error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}")
+    }
+
+    return [ metas[0], fastqs ]
+}
+//
+// Get attribute from genome config file e.g. fasta
+//
+def getGenomeAttribute(attribute) {
+    if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
+        if (params.genomes[ params.genome ].containsKey(attribute)) {
+            return params.genomes[ params.genome ][ attribute ]
+        }
+    }
+    return null
+}
+
+//
+// Exit pipeline if incorrect --genome key provided
+//
+def genomeExistsError() {
+    if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
+        def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
+            "  Currently, the available genome keys are:\n" +
+            "  ${params.genomes.keySet().join(", ")}\n" +
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        error(error_string)
+    }
+}
+
+//
+// Generate methods description for MultiQC
+//
+def toolCitationText() {
+    // TODO nf-core: Optionally add in-text citation tools to this list.
+    // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
+    // Uncomment function in methodsDescriptionText to render in MultiQC report
+    def citation_text = [
+            "Tools used in the workflow included:",
+            "FastQC (Andrews 2010),",
+            "MultiQC (Ewels et al. 2016)",
+            "."
+        ].join(' ').trim()
+
+    return citation_text
+}
+
+def toolBibliographyText() {
+    // TODO nf-core: Optionally add bibliographic entries to this list.
+    // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "<li>Author (2023) Pub name, Journal, DOI</li>" : "",
+    // Uncomment function in methodsDescriptionText to render in MultiQC report
+    def reference_text = [
+            "<li>Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).</li>",
+            "<li>Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354</li>"
+        ].join(' ').trim()
+
+    return reference_text
+}
+
+def methodsDescriptionText(mqc_methods_yaml) {
+    // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
+    def meta = [:]
+    meta.workflow = workflow.toMap()
+    meta["manifest_map"] = workflow.manifest.toMap()
+
+    // Pipeline DOI
+    meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
+    meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
+
+    // Tool references
+    meta["tool_citations"] = ""
+    meta["tool_bibliography"] = ""
+
+    // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
+    // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".")
+    // meta["tool_bibliography"] = toolBibliographyText()
+
+
+    def methods_text = mqc_methods_yaml.text
+
+    def engine =  new groovy.text.SimpleTemplateEngine()
+    def description_html = engine.createTemplate(methods_text).make(meta)
+
+    return description_html.toString()
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
new file mode 100644
index 00000000..ac31f28f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -0,0 +1,126 @@
+//
+// Subworkflow with functionality that may be useful for any Nextflow pipeline
+//
+
+import org.yaml.snakeyaml.Yaml
+import groovy.json.JsonOutput
+import nextflow.extension.FilesEx
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NEXTFLOW_PIPELINE {
+
+    take:
+    print_version        // boolean: print version
+    dump_parameters      // boolean: dump parameters
+    outdir               //    path: base directory used to publish pipeline results
+    check_conda_channels // boolean: check conda channels
+
+    main:
+
+    //
+    // Print workflow version and exit on --version
+    //
+    if (print_version) {
+        log.info "${workflow.manifest.name} ${getWorkflowVersion()}"
+        System.exit(0)
+    }
+
+    //
+    // Dump pipeline parameters to a JSON file
+    //
+    if (dump_parameters && outdir) {
+        dumpParametersToJSON(outdir)
+    }
+
+    //
+    // When running with Conda, warn if channels have not been set-up appropriately
+    //
+    if (check_conda_channels) {
+        checkCondaChannels()
+    }
+
+    emit:
+    dummy_emit = true
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+
+//
+// Generate version string
+//
+def getWorkflowVersion() {
+    String version_string = ""
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Dump pipeline parameters to a JSON file
+//
+def dumpParametersToJSON(outdir) {
+    def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
+    def filename   = "params_${timestamp}.json"
+    def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
+    def jsonStr    = JsonOutput.toJson(params)
+    temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
+
+    FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
+    temp_pf.delete()
+}
+
+//
+// When running with -profile conda, warn if channels have not been set-up appropriately
+//
+def checkCondaChannels() {
+    Yaml parser = new Yaml()
+    def channels = []
+    try {
+        def config = parser.load("conda config --show channels".execute().text)
+        channels = config.channels
+    } catch(NullPointerException | IOException e) {
+        log.warn "Could not verify conda channel configuration."
+        return
+    }
+
+    // Check that all channels are present
+    // This channel list is ordered by required channel priority.
+    def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
+    def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
+
+    // Check that they are in the right order
+    def channel_priority_violation = false
+    def n = required_channels_in_order.size()
+    for (int i = 0; i < n - 1; i++) {
+        channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
+    }
+
+    if (channels_missing | channel_priority_violation) {
+        log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            "  There is a problem with your Conda configuration!\n\n" +
+            "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
+            "  Please refer to https://bioconda.github.io/\n" +
+            "  The observed channel order is \n" +
+            "  ${channels}\n" +
+            "  but the following channel order is required:\n" +
+            "  ${required_channels_in_order}\n" +
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
new file mode 100644
index 00000000..e5c3a0a8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
@@ -0,0 +1,38 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NEXTFLOW_PIPELINE"
+description: Subworkflow with functionality that may be useful for any Nextflow pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - print_version:
+      type: boolean
+      description: |
+        Print the version of the pipeline and exit
+  - dump_parameters:
+      type: boolean
+      description: |
+        Dump the parameters of the pipeline to a JSON file
+  - output_directory:
+      type: directory
+      description: Path to output dir to write JSON file to.
+      pattern: "results/"
+  - check_conda_channel:
+      type: boolean
+      description: |
+        Check if the conda channel priority is correct.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+  - "@drpatelh"
+maintainers:
+  - "@adamrtalbot"
+  - "@drpatelh"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..68718e4f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
@@ -0,0 +1,54 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Test Function getWorkflowVersion") {
+
+        function "getWorkflowVersion"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dumpParametersToJSON") {
+
+        function "dumpParametersToJSON"
+
+        when {
+            function {
+                """
+                // define inputs of the function here. Example:
+                input[0] = "$outputDir"
+                """.stripIndent()
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success }
+            )
+        }
+    }
+
+    test("Test Function checkCondaChannels") {
+
+        function "checkCondaChannels"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..e3f0baf4
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,20 @@
+{
+    "Test Function getWorkflowVersion": {
+        "content": [
+            "v9.9.9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:05.308243"
+    },
+    "Test Function checkCondaChannels": {
+        "content": null,
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:12.425833"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..ca964ce8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,111 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NEXTFLOW_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    workflow "UTILS_NEXTFLOW_PIPELINE"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Should run no inputs") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should print version") {
+
+        when {
+            workflow {
+                """
+                print_version        = true
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.stdout.contains("nextflow_workflow v9.9.9") }
+            )
+        }
+    }
+
+    test("Should dump params") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = 'results'
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should not create params JSON if no output directory") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..d0a926bf
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
new file mode 100644
index 00000000..f8476112
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nextflow_pipeline:
+  - subworkflows/nf-core/utils_nextflow_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
new file mode 100644
index 00000000..a8b55d6f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -0,0 +1,440 @@
+//
+// Subworkflow with utility functions specific to the nf-core pipeline template
+//
+
+import org.yaml.snakeyaml.Yaml
+import nextflow.extension.FilesEx
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NFCORE_PIPELINE {
+
+    take:
+    nextflow_cli_args
+
+    main:
+    valid_config = checkConfigProvided()
+    checkProfileProvided(nextflow_cli_args)
+
+    emit:
+    valid_config
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+
+//
+//  Warn if a -profile or Nextflow config has not been provided to run the pipeline
+//
+def checkConfigProvided() {
+    valid_config = true
+    if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
+        log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
+            "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
+            "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
+            "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
+            "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
+            "Please refer to the quick start section and usage docs for the pipeline.\n "
+        valid_config = false
+    }
+    return valid_config
+}
+
+//
+// Exit pipeline if --profile contains spaces
+//
+def checkProfileProvided(nextflow_cli_args) {
+    if (workflow.profile.endsWith(',')) {
+        error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" +
+            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+    }
+    if (nextflow_cli_args[0]) {
+        log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" +
+            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+    }
+}
+
+//
+// Citation string for pipeline
+//
+def workflowCitation() {
+    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
+        "* The pipeline\n" +
+        "  ${workflow.manifest.doi}\n\n" +
+        "* The nf-core framework\n" +
+        "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
+        "* Software dependencies\n" +
+        "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
+}
+
+//
+// Generate workflow version string
+//
+def getWorkflowVersion() {
+    String version_string = ""
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Get software versions for pipeline
+//
+def processVersionsFromYAML(yaml_file) {
+    Yaml yaml = new Yaml()
+    versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] }
+    return yaml.dumpAsMap(versions).trim()
+}
+
+//
+// Get workflow version for pipeline
+//
+def workflowVersionToYAML() {
+    return """
+    Workflow:
+        $workflow.manifest.name: ${getWorkflowVersion()}
+        Nextflow: $workflow.nextflow.version
+    """.stripIndent().trim()
+}
+
+//
+// Get channel of software versions used in pipeline in YAML format
+//
+def softwareVersionsToYAML(ch_versions) {
+    return ch_versions
+                .unique()
+                .map { processVersionsFromYAML(it) }
+                .unique()
+                .mix(Channel.of(workflowVersionToYAML()))
+}
+
+//
+// Get workflow summary for MultiQC
+//
+def paramsSummaryMultiqc(summary_params) {
+    def summary_section = ''
+    for (group in summary_params.keySet()) {
+        def group_params = summary_params.get(group)  // This gets the parameters of that particular group
+        if (group_params) {
+            summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
+            summary_section += "    <dl class=\"dl-horizontal\">\n"
+            for (param in group_params.keySet()) {
+                summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+            }
+            summary_section += "    </dl>\n"
+        }
+    }
+
+    String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
+    yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
+    yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+    yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+    yaml_file_text        += "plot_type: 'html'\n"
+    yaml_file_text        += "data: |\n"
+    yaml_file_text        += "${summary_section}"
+
+    return yaml_file_text
+}
+
+//
+// nf-core logo
+//
+def nfCoreLogo(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    String.format(
+        """\n
+        ${dashedLine(monochrome_logs)}
+                                                ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
+        ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
+        ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
+        ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
+                                                ${colors.green}`._,._,\'${colors.reset}
+        ${colors.purple}  ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset}
+        ${dashedLine(monochrome_logs)}
+        """.stripIndent()
+    )
+}
+
+//
+// Return dashed line
+//
+def dashedLine(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+}
+
+//
+// ANSII colours used for terminal logging
+//
+def logColours(monochrome_logs=true) {
+    Map colorcodes = [:]
+
+    // Reset / Meta
+    colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
+    colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
+    colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
+    colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
+    colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
+    colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
+    colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
+
+    // Regular Colors
+    colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
+    colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
+    colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
+    colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
+    colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
+    colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
+    colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
+    colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
+
+    // Bold
+    colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
+    colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
+    colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
+    colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
+    colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
+    colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
+    colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
+    colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
+
+    // Underline
+    colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
+    colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
+    colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
+    colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
+    colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
+    colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
+    colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
+    colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
+
+    // High Intensity
+    colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
+    colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
+    colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
+    colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
+    colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
+    colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
+    colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
+    colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
+
+    // Bold High Intensity
+    colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
+    colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
+    colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
+    colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
+    colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
+    colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
+    colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
+    colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
+
+    return colorcodes
+}
+
+//
+// Attach the multiqc report to email
+//
+def attachMultiqcReport(multiqc_report) {
+    def mqc_report = null
+    try {
+        if (workflow.success) {
+            mqc_report = multiqc_report.getVal()
+            if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
+                if (mqc_report.size() > 1) {
+                    log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
+                }
+                mqc_report = mqc_report[0]
+            }
+        }
+    } catch (all) {
+        if (multiqc_report) {
+            log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
+        }
+    }
+    return mqc_report
+}
+
+//
+// Construct and send completion email
+//
+def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) {
+
+    // Set up the e-mail variables
+    def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
+    if (!workflow.success) {
+        subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
+    }
+
+    def summary = [:]
+    for (group in summary_params.keySet()) {
+        summary << summary_params[group]
+    }
+
+    def misc_fields = [:]
+    misc_fields['Date Started']              = workflow.start
+    misc_fields['Date Completed']            = workflow.complete
+    misc_fields['Pipeline script file path'] = workflow.scriptFile
+    misc_fields['Pipeline script hash ID']   = workflow.scriptId
+    if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
+    if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+    if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
+    misc_fields['Nextflow Version']           = workflow.nextflow.version
+    misc_fields['Nextflow Build']             = workflow.nextflow.build
+    misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
+
+    def email_fields = [:]
+    email_fields['version']      = getWorkflowVersion()
+    email_fields['runName']      = workflow.runName
+    email_fields['success']      = workflow.success
+    email_fields['dateComplete'] = workflow.complete
+    email_fields['duration']     = workflow.duration
+    email_fields['exitStatus']   = workflow.exitStatus
+    email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    email_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    email_fields['commandLine']  = workflow.commandLine
+    email_fields['projectDir']   = workflow.projectDir
+    email_fields['summary']      = summary << misc_fields
+
+    // On success try attach the multiqc report
+    def mqc_report = attachMultiqcReport(multiqc_report)
+
+    // Check if we are only sending emails on failure
+    def email_address = email
+    if (!email && email_on_fail && !workflow.success) {
+        email_address = email_on_fail
+    }
+
+    // Render the TXT template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    def tf           = new File("${workflow.projectDir}/assets/email_template.txt")
+    def txt_template = engine.createTemplate(tf).make(email_fields)
+    def email_txt    = txt_template.toString()
+
+    // Render the HTML template
+    def hf            = new File("${workflow.projectDir}/assets/email_template.html")
+    def html_template = engine.createTemplate(hf).make(email_fields)
+    def email_html    = html_template.toString()
+
+    // Render the sendmail template
+    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
+    def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
+    def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
+    def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
+    def sendmail_html          = sendmail_template.toString()
+
+    // Send the HTML e-mail
+    Map colors = logColours(monochrome_logs)
+    if (email_address) {
+        try {
+            if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
+            // Try to send HTML e-mail using sendmail
+            def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
+            sendmail_tf.withWriter { w -> w << sendmail_html }
+            [ 'sendmail', '-t' ].execute() << sendmail_html
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
+        } catch (all) {
+            // Catch failures and try with plaintext
+            def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+            mail_cmd.execute() << email_html
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
+        }
+    }
+
+    // Write summary e-mail HTML to a file
+    def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
+    output_hf.withWriter { w -> w << email_html }
+    FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html");
+    output_hf.delete()
+
+    // Write summary e-mail TXT to a file
+    def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
+    output_tf.withWriter { w -> w << email_txt }
+    FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt");
+    output_tf.delete()
+}
+
+//
+// Print pipeline summary on completion
+//
+def completionSummary(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    if (workflow.success) {
+        if (workflow.stats.ignoredCount == 0) {
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
+        } else {
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+        }
+    } else {
+        log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
+    }
+}
+
+//
+// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack
+//
+def imNotification(summary_params, hook_url) {
+    def summary = [:]
+    for (group in summary_params.keySet()) {
+        summary << summary_params[group]
+    }
+
+    def misc_fields = [:]
+    misc_fields['start']                                = workflow.start
+    misc_fields['complete']                             = workflow.complete
+    misc_fields['scriptfile']                           = workflow.scriptFile
+    misc_fields['scriptid']                             = workflow.scriptId
+    if (workflow.repository) misc_fields['repository']  = workflow.repository
+    if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
+    if (workflow.revision)   misc_fields['revision']    = workflow.revision
+    misc_fields['nxf_version']                          = workflow.nextflow.version
+    misc_fields['nxf_build']                            = workflow.nextflow.build
+    misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
+
+    def msg_fields = [:]
+    msg_fields['version']      = getWorkflowVersion()
+    msg_fields['runName']      = workflow.runName
+    msg_fields['success']      = workflow.success
+    msg_fields['dateComplete'] = workflow.complete
+    msg_fields['duration']     = workflow.duration
+    msg_fields['exitStatus']   = workflow.exitStatus
+    msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
+    msg_fields['projectDir']   = workflow.projectDir
+    msg_fields['summary']      = summary << misc_fields
+
+    // Render the JSON template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    // Different JSON depending on the service provider
+    // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
+    def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
+    def hf            = new File("${workflow.projectDir}/assets/${json_path}")
+    def json_template = engine.createTemplate(hf).make(msg_fields)
+    def json_message  = json_template.toString()
+
+    // POST
+    def post = new URL(hook_url).openConnection();
+    post.setRequestMethod("POST")
+    post.setDoOutput(true)
+    post.setRequestProperty("Content-Type", "application/json")
+    post.getOutputStream().write(json_message.getBytes("UTF-8"));
+    def postRC = post.getResponseCode();
+    if (! postRC.equals(200)) {
+        log.warn(post.getErrorStream().getText());
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
new file mode 100644
index 00000000..d08d2434
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
@@ -0,0 +1,24 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFCORE_PIPELINE"
+description: Subworkflow with utility functions specific to the nf-core pipeline template
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - nextflow_cli_args:
+      type: list
+      description: |
+        Nextflow CLI positional arguments
+output:
+  - success:
+      type: boolean
+      description: |
+        Dummy output to indicate success
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..1dc317f8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
@@ -0,0 +1,134 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Test Function checkConfigProvided") {
+
+        function "checkConfigProvided"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function checkProfileProvided") {
+
+        function "checkProfileProvided"
+
+        when {
+            function {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function workflowCitation") {
+
+        function "workflowCitation"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function nfCoreLogo") {
+
+        function "nfCoreLogo"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dashedLine") {
+
+        function "dashedLine"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function without logColours") {
+
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function with logColours") {
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..1037232c
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,166 @@
+{
+    "Test Function checkProfileProvided": {
+        "content": null,
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:03.360873"
+    },
+    "Test Function checkConfigProvided": {
+        "content": [
+            true
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:59.729647"
+    },
+    "Test Function nfCoreLogo": {
+        "content": [
+            "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n                                        \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m        ___     __   __   __   ___     \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \u001b[0;33m}  {\u001b[0m\n\u001b[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \u001b[0;32m\\`-._,-`-,\u001b[0m\n                                        \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m  nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:10.562934"
+    },
+    "Test Function workflowCitation": {
+        "content": [
+            "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n  https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n  https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n  https://github.com/nextflow_workflow/blob/master/CITATIONS.md"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:07.019761"
+    },
+    "Test Function without logColours": {
+        "content": [
+            {
+                "reset": "",
+                "bold": "",
+                "dim": "",
+                "underlined": "",
+                "blink": "",
+                "reverse": "",
+                "hidden": "",
+                "black": "",
+                "red": "",
+                "green": "",
+                "yellow": "",
+                "blue": "",
+                "purple": "",
+                "cyan": "",
+                "white": "",
+                "bblack": "",
+                "bred": "",
+                "bgreen": "",
+                "byellow": "",
+                "bblue": "",
+                "bpurple": "",
+                "bcyan": "",
+                "bwhite": "",
+                "ublack": "",
+                "ured": "",
+                "ugreen": "",
+                "uyellow": "",
+                "ublue": "",
+                "upurple": "",
+                "ucyan": "",
+                "uwhite": "",
+                "iblack": "",
+                "ired": "",
+                "igreen": "",
+                "iyellow": "",
+                "iblue": "",
+                "ipurple": "",
+                "icyan": "",
+                "iwhite": "",
+                "biblack": "",
+                "bired": "",
+                "bigreen": "",
+                "biyellow": "",
+                "biblue": "",
+                "bipurple": "",
+                "bicyan": "",
+                "biwhite": ""
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:17.969323"
+    },
+    "Test Function dashedLine": {
+        "content": [
+            "-\u001b[2m----------------------------------------------------\u001b[0m-"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:14.366181"
+    },
+    "Test Function with logColours": {
+        "content": [
+            {
+                "reset": "\u001b[0m",
+                "bold": "\u001b[1m",
+                "dim": "\u001b[2m",
+                "underlined": "\u001b[4m",
+                "blink": "\u001b[5m",
+                "reverse": "\u001b[7m",
+                "hidden": "\u001b[8m",
+                "black": "\u001b[0;30m",
+                "red": "\u001b[0;31m",
+                "green": "\u001b[0;32m",
+                "yellow": "\u001b[0;33m",
+                "blue": "\u001b[0;34m",
+                "purple": "\u001b[0;35m",
+                "cyan": "\u001b[0;36m",
+                "white": "\u001b[0;37m",
+                "bblack": "\u001b[1;30m",
+                "bred": "\u001b[1;31m",
+                "bgreen": "\u001b[1;32m",
+                "byellow": "\u001b[1;33m",
+                "bblue": "\u001b[1;34m",
+                "bpurple": "\u001b[1;35m",
+                "bcyan": "\u001b[1;36m",
+                "bwhite": "\u001b[1;37m",
+                "ublack": "\u001b[4;30m",
+                "ured": "\u001b[4;31m",
+                "ugreen": "\u001b[4;32m",
+                "uyellow": "\u001b[4;33m",
+                "ublue": "\u001b[4;34m",
+                "upurple": "\u001b[4;35m",
+                "ucyan": "\u001b[4;36m",
+                "uwhite": "\u001b[4;37m",
+                "iblack": "\u001b[0;90m",
+                "ired": "\u001b[0;91m",
+                "igreen": "\u001b[0;92m",
+                "iyellow": "\u001b[0;93m",
+                "iblue": "\u001b[0;94m",
+                "ipurple": "\u001b[0;95m",
+                "icyan": "\u001b[0;96m",
+                "iwhite": "\u001b[0;97m",
+                "biblack": "\u001b[1;90m",
+                "bired": "\u001b[1;91m",
+                "bigreen": "\u001b[1;92m",
+                "biyellow": "\u001b[1;93m",
+                "biblue": "\u001b[1;94m",
+                "bipurple": "\u001b[1;95m",
+                "bicyan": "\u001b[1;96m",
+                "biwhite": "\u001b[1;97m"
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:21.714424"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..8940d32d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,29 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFCORE_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    workflow "UTILS_NFCORE_PIPELINE"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Should run without failures") {
+
+        when {
+            workflow {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
new file mode 100644
index 00000000..859d1030
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
@@ -0,0 +1,19 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    true
+                ],
+                "valid_config": [
+                    true
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:25.726491"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..d0a926bf
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
new file mode 100644
index 00000000..ac8523c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfcore_pipeline:
+  - subworkflows/nf-core/utils_nfcore_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
new file mode 100644
index 00000000..2585b65d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
@@ -0,0 +1,62 @@
+//
+// Subworkflow that uses the nf-validation plugin to render help text and parameter summary
+//
+
+/*
+========================================================================================
+    IMPORT NF-VALIDATION PLUGIN
+========================================================================================
+*/
+
+include { paramsHelp         } from 'plugin/nf-validation'
+include { paramsSummaryLog   } from 'plugin/nf-validation'
+include { validateParameters } from 'plugin/nf-validation'
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NFVALIDATION_PLUGIN {
+
+    take:
+    print_help       // boolean: print help
+    workflow_command //  string: default commmand used to run pipeline
+    pre_help_text    //  string: string to be printed before help text and summary log
+    post_help_text   //  string: string to be printed after help text and summary log
+    validate_params  // boolean: validate parameters
+    schema_filename  //    path: JSON schema file, null to use default value
+
+    main:
+
+    log.debug "Using schema file: ${schema_filename}"
+
+    // Default values for strings
+    pre_help_text    = pre_help_text    ?: ''
+    post_help_text   = post_help_text   ?: ''
+    workflow_command = workflow_command ?: ''
+
+    //
+    // Print help message if needed
+    //
+    if (print_help) {
+        log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text
+        System.exit(0)
+    }
+
+    //
+    // Print parameter summary to stdout
+    //
+    log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text
+
+    //
+    // Validate parameters relative to the parameter JSON schema
+    //
+    if (validate_params){
+        validateParameters(parameters_schema: schema_filename)
+    }
+
+    emit:
+    dummy_emit = true
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
new file mode 100644
index 00000000..3d4a6b04
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
@@ -0,0 +1,44 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFVALIDATION_PLUGIN"
+description: Use nf-validation to initiate and validate a pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - validation
+components: []
+input:
+  - print_help:
+      type: boolean
+      description: |
+        Print help message and exit
+  - workflow_command:
+      type: string
+      description: |
+        The command to run the workflow e.g. "nextflow run main.nf"
+  - pre_help_text:
+      type: string
+      description: |
+        Text to print before the help message
+  - post_help_text:
+      type: string
+      description: |
+        Text to print after the help message
+  - validate_params:
+      type: boolean
+      description: |
+        Validate the parameters and error if invalid.
+  - schema_filename:
+      type: string
+      description: |
+        The filename of the schema to validate against.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
new file mode 100644
index 00000000..5784a33f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
@@ -0,0 +1,200 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFVALIDATION_PLUGIN"
+    script "../main.nf"
+    workflow "UTILS_NFVALIDATION_PLUGIN"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "plugin/nf-validation"
+    tag "'plugin/nf-validation'"
+    tag "utils_nfvalidation_plugin"
+    tag "subworkflows/utils_nfvalidation_plugin"
+
+    test("Should run nothing") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+
+            workflow {
+                """
+                help             = false
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should run help") {
+
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } }
+            )
+        }
+    }
+
+    test("Should run help with command") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = "nextflow run noorg/doesntexist"
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } }
+            )
+        }
+    }
+
+    test("Should run help with extra text") {
+
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = "nextflow run noorg/doesntexist"
+                pre_help_text    = "pre-help-text"
+                post_help_text   = "post-help-text"
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('pre-help-text') } },
+                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } },
+                { assert workflow.stdout.any { it.contains('post-help-text') } }
+            )
+        }
+    }
+
+    test("Should validate params") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+                outdir          = 1
+            }
+            workflow {
+                """
+                help             = false
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = true
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
new file mode 100644
index 00000000..7626c1c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
@@ -0,0 +1,96 @@
+{
+    "$schema": "http://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json",
+    "title": ". pipeline parameters",
+    "description": "",
+    "type": "object",
+    "definitions": {
+        "input_output_options": {
+            "title": "Input/output options",
+            "type": "object",
+            "fa_icon": "fas fa-terminal",
+            "description": "Define where the pipeline should find input data and save output data.",
+            "required": ["outdir"],
+            "properties": {
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Validate parameters?",
+                    "default": true,
+                    "hidden": true
+                },
+                "outdir": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "test_data_base": {
+                    "type": "string",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules",
+                    "description": "Base for test data directory",
+                    "hidden": true
+                },
+                "test_data": {
+                    "type": "string",
+                    "description": "Fake test data param",
+                    "hidden": true
+                }
+            }
+        },
+        "generic_options": {
+            "title": "Generic options",
+            "type": "object",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "version": {
+                    "type": "boolean",
+                    "description": "Display version and exit.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "logo": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Display nf-core logo in console output.",
+                    "fa_icon": "fas fa-image",
+                    "hidden": true
+                },
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Pull Singularity container from Docker?",
+                    "hidden": true
+                },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "hidden": true
+                },
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Use monochrome_logs",
+                    "hidden": true
+                }
+            }
+        }
+    },
+    "allOf": [
+        {
+            "$ref": "#/definitions/input_output_options"
+        },
+        {
+            "$ref": "#/definitions/generic_options"
+        }
+    ]
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
new file mode 100644
index 00000000..60b1cfff
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfvalidation_plugin:
+  - subworkflows/nf-core/utils_nfvalidation_plugin/**
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index db0a1dd5..42da5dd5 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -1,54 +1,15 @@
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    PRINT PARAMS SUMMARY
+    IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation'
-
-def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-def summary_params = paramsSummaryMap(workflow)
-
-// Print parameter summary log to screen
-log.info logo + paramsSummaryLog(workflow) + citation
-
-WorkflowAssemblyqc.initialise(params, log)
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    CONFIG FILES
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-ch_multiqc_config          = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-ch_multiqc_custom_config   = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty()
-ch_multiqc_logo            = params.multiqc_logo   ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty()
-ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
-//
-include { INPUT_CHECK } from '../subworkflows/local/input_check'
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// MODULE: Installed directly from nf-core/modules
-//
-include { FASTQC                      } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
+include { FASTQC                 } from '../modules/nf-core/fastqc/main'
+include { MULTIQC                } from '../modules/nf-core/multiqc/main'
+include { paramsSummaryMap       } from 'plugin/nf-validation'
+include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -56,50 +17,67 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-// Info required for completion email and summary
-def multiqc_report = []
-
 workflow ASSEMBLYQC {
 
-    ch_versions = Channel.empty()
+    take:
+    ch_samplesheet // channel: samplesheet read in from --input
 
-    //
-    // SUBWORKFLOW: Read in samplesheet, validate and stage input files
-    //
-    INPUT_CHECK (
-        file(params.input)
-    )
-    ch_versions = ch_versions.mix(INPUT_CHECK.out.versions)
-    // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input")
-    // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/
-    // ! There is currently no tooling to help you write a sample sheet schema
+    main:
+
+    ch_versions = Channel.empty()
+    ch_multiqc_files = Channel.empty()
 
     //
     // MODULE: Run FastQC
     //
     FASTQC (
-        INPUT_CHECK.out.reads
+        ch_samplesheet
     )
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
     ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
-    CUSTOM_DUMPSOFTWAREVERSIONS (
-        ch_versions.unique().collectFile(name: 'collated_versions.yml')
-    )
+    //
+    // Collate and save software versions
+    //
+    softwareVersionsToYAML(ch_versions)
+        .collectFile(
+            storeDir: "${params.outdir}/pipeline_info",
+            name: 'nf_core_pipeline_software_mqc_versions.yml',
+            sort: true,
+            newLine: true
+        ).set { ch_collated_versions }
 
     //
     // MODULE: MultiQC
     //
-    workflow_summary    = WorkflowAssemblyqc.paramsSummaryMultiqc(workflow, summary_params)
-    ch_workflow_summary = Channel.value(workflow_summary)
-
-    methods_description    = WorkflowAssemblyqc.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params)
-    ch_methods_description = Channel.value(methods_description)
-
-    ch_multiqc_files = Channel.empty()
-    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_config        = Channel.fromPath(
+        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config = params.multiqc_config ?
+        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        Channel.empty()
+    ch_multiqc_logo          = params.multiqc_logo ?
+        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        Channel.empty()
+
+    summary_params      = paramsSummaryMap(
+        workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+        file(params.multiqc_methods_description, checkIfExists: true) :
+        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(
+        methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_methods_description.collectFile(
+            name: 'methods_description_mqc.yaml',
+            sort: false
+        )
+    )
 
     MULTIQC (
         ch_multiqc_files.collect(),
@@ -107,24 +85,10 @@ workflow ASSEMBLYQC {
         ch_multiqc_custom_config.toList(),
         ch_multiqc_logo.toList()
     )
-    multiqc_report = MULTIQC.out.report.toList()
-}
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    COMPLETION EMAIL AND SUMMARY
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
 
-workflow.onComplete {
-    if (params.email || params.email_on_fail) {
-        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
-    }
-    NfcoreTemplate.dump_parameters(workflow, params)
-    NfcoreTemplate.summary(workflow, params, log)
-    if (params.hook_url) {
-        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-    }
+    emit:
+    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+    versions       = ch_versions                 // channel: [ path(versions.yml) ]
 }
 
 /*

From a1fda61aa44357e072e691989d6cf18d05601a68 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 13:00:56 +1200
Subject: [PATCH 04/76] Merged TEMPLATE

---
 .devcontainer/devcontainer.json               |  10 +-
 .editorconfig                                 |  10 +-
 .github/CONTRIBUTING.md                       |   9 +-
 .github/PULL_REQUEST_TEMPLATE.md              |   4 +-
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/ci.yml                      |   7 +-
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  35 +-
 .github/workflows/fix-linting.yml             |  59 ++-
 .github/workflows/linting.yml                 |  74 +--
 .github/workflows/linting_comment.yml         |   4 +-
 .github/workflows/release-announcements.yml   |  75 +++
 .gitpod.yml                                   |   6 +-
 .nf-core.yml                                  |   1 +
 .pre-commit-config.yaml                       |   3 +
 CHANGELOG.md                                  |   2 +
 README.md                                     |   7 +-
 assets/multiqc_config.yml                     |  16 +
 conf/base.config                              |   3 -
 conf/modules.config                           |   8 -
 lib/NfcoreTemplate.groovy                     | 351 --------------
 lib/Utils.groovy                              |  47 --
 lib/WorkflowAssemblyqc.groovy                 | 100 ----
 lib/WorkflowMain.groovy                       |  57 ---
 main.nf                                       |  71 +--
 modules.json                                  | 138 ++++--
 .../dumpsoftwareversions/environment.yml      |   7 -
 .../custom/dumpsoftwareversions/main.nf       |  24 -
 .../custom/dumpsoftwareversions/meta.yml      |  37 --
 .../templates/dumpsoftwareversions.py         | 101 ----
 .../dumpsoftwareversions/tests/main.nf.test   |  43 --
 .../tests/main.nf.test.snap                   |  33 --
 .../dumpsoftwareversions/tests/tags.yml       |   2 -
 modules/nf-core/gunzip/environment.yml        |   2 +-
 modules/nf-core/multiqc/main.nf               |  55 +++
 modules/nf-core/multiqc/meta.yml              |  58 +++
 modules/nf-core/multiqc/tests/main.nf.test    |  84 ++++
 .../nf-core/multiqc/tests/main.nf.test.snap   |  41 ++
 nextflow_schema.json                          |   4 +-
 pyproject.toml                                |  13 -
 .../utils_nfcore_assemblyqc_pipeline/main.nf  | 219 +++++++++
 .../nf-core/utils_nextflow_pipeline/main.nf   | 126 +++++
 .../nf-core/utils_nextflow_pipeline/meta.yml  |  38 ++
 .../tests/main.function.nf.test               |  54 +++
 .../tests/main.function.nf.test.snap          |  20 +
 .../tests/main.workflow.nf.test               | 111 +++++
 .../tests/nextflow.config                     |   9 +
 .../utils_nextflow_pipeline/tests/tags.yml    |   2 +
 .../nf-core/utils_nfcore_pipeline/main.nf     | 440 ++++++++++++++++++
 .../nf-core/utils_nfcore_pipeline/meta.yml    |  24 +
 .../tests/main.function.nf.test               | 134 ++++++
 .../tests/main.function.nf.test.snap          | 166 +++++++
 .../tests/main.workflow.nf.test               |  29 ++
 .../tests/main.workflow.nf.test.snap          |  19 +
 .../tests/nextflow.config                     |   9 +
 .../utils_nfcore_pipeline/tests/tags.yml      |   2 +
 .../nf-core/utils_nfvalidation_plugin/main.nf |  62 +++
 .../utils_nfvalidation_plugin/meta.yml        |  44 ++
 .../tests/main.nf.test                        | 200 ++++++++
 .../tests/nextflow_schema.json                |  96 ++++
 .../utils_nfvalidation_plugin/tests/tags.yml  |   2 +
 workflows/assemblyqc.nf                       |  86 +---
 62 files changed, 2458 insertions(+), 1039 deletions(-)
 create mode 100644 .github/workflows/release-announcements.yml
 create mode 100644 assets/multiqc_config.yml
 delete mode 100755 lib/NfcoreTemplate.groovy
 delete mode 100644 lib/Utils.groovy
 delete mode 100755 lib/WorkflowAssemblyqc.groovy
 delete mode 100755 lib/WorkflowMain.groovy
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml
 delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
 create mode 100644 modules/nf-core/multiqc/main.nf
 create mode 100644 modules/nf-core/multiqc/meta.yml
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap
 delete mode 100644 pyproject.toml
 create mode 100644 subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
index 4ecfbfe3..b290e090 100644
--- a/.devcontainer/devcontainer.json
+++ b/.devcontainer/devcontainer.json
@@ -10,15 +10,7 @@
         "vscode": {
             // Set *default* container specific settings.json values on container create.
             "settings": {
-                "python.defaultInterpreterPath": "/opt/conda/bin/python",
-                "python.linting.enabled": true,
-                "python.linting.pylintEnabled": true,
-                "python.formatting.autopep8Path": "/opt/conda/bin/autopep8",
-                "python.formatting.yapfPath": "/opt/conda/bin/yapf",
-                "python.linting.flake8Path": "/opt/conda/bin/flake8",
-                "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle",
-                "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle",
-                "python.linting.pylintPath": "/opt/conda/bin/pylint"
+                "python.defaultInterpreterPath": "/opt/conda/bin/python"
             },
 
             // Add the IDs of extensions you want installed when the container is created.
diff --git a/.editorconfig b/.editorconfig
index eb77d1dd..83ee163c 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -18,16 +18,12 @@ end_of_line = unset
 insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
-indent_size = unset
-
-# These files are edited and tested upstream in nf-core/subworkflows
 [/subworkflows/nf-core/**]
 charset = unset
 end_of_line = unset
 insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
-indent_size = unset
 
 # These files are edited and tested upstream in pfr/modules
 [/modules/pfr/**]
@@ -36,16 +32,12 @@ end_of_line = unset
 insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
-indent_size = unset
-
-# These files are edited and tested upstream in pfr/subworkflows
 [/subworkflows/pfr/**]
 charset = unset
 end_of_line = unset
 insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
-indent_size = unset
 
 [/assets/email*]
 indent_size = unset
@@ -55,7 +47,7 @@ indent_size = unset
 indent_style = unset
 
 # ignore python
-[*.{py}]
+[*.{py,md}]
 indent_style = unset
 
 # ignore perl
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index edd71437..b8059542 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -23,8 +23,11 @@ If you're not used to this workflow with git, you can start with some [docs from
 
 ## Tests
 
-You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to
-receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir <OUTDIR>`.
+You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command:
+
+```bash
+nf-test test --profile debug,test,docker --verbose
+```
 
 When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
 Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
@@ -81,7 +84,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`.
 
 Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
 
-The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block.
+The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block.
 
 ### Naming schemes
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 50053a1f..86930edb 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -16,8 +16,8 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/plant-food-r
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md)
-- [ ] Make sure your code lints (`nf-core lint`) and (`pre-commit run --all`)
-- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
+- [ ] Make sure your code lints (`nf-core lint`).
+- [ ] Ensure the test suite passes (`nf-test test tests/ --verbose --profile +docker`).
 - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 752ea1bf..9186934e 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -19,7 +19,7 @@ jobs:
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
-        uses: mshick/add-pr-comment@v2
+        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
         with:
           message: |
             ## This PR is against the `master` branch :x:
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 4ff4e1f8..6cacb7d0 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -31,13 +31,16 @@ jobs:
           - "-profile docker -params-file ./tests/invalid/params.json"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v4
+        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
         with:
           version: ${{ matrix.NXF_VER }}
 
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
       - name: Run pipeline with test data
         run: |
           nextflow run ${GITHUB_WORKSPACE} --outdir ./results ${{ matrix.RUN_CONFIG }}
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index e37cfda5..0b6b1f27 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@v9
+      - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 69574376..2d20d644 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -6,9 +6,16 @@ name: Test successful pipeline download with 'nf-core download'
 #  - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev.
 on:
   workflow_dispatch:
+    inputs:
+      testbranch:
+        description: "The specific branch you wish to utilize for the test execution of nf-core download."
+        required: true
+        default: "dev"
   pull_request:
     types:
       - opened
+      - edited
+      - synchronize
     branches:
       - master
   pull_request_target:
@@ -20,16 +27,19 @@ env:
 
 jobs:
   download:
-    runs-on: ["self-hosted"]
+    runs-on: ubuntu-latest
     steps:
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@v5
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
-      - uses: eWaterCycle/setup-singularity@v7
+      - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
         with:
           singularity-version: 3.8.3
 
@@ -42,13 +52,13 @@ jobs:
         run: |
           echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
           echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
-          echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV}
+          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}
 
       - name: Download the pipeline
         env:
           NXF_SINGULARITY_CACHEDIR: ./
         run: |
-          nf-core download  ${{ env.REPO_LOWERCASE }} \
+          nf-core download ${{ env.REPO_LOWERCASE }} \
           --revision ${{ env.REPO_BRANCH }} \
           --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
           --compress "none" \
@@ -60,8 +70,17 @@ jobs:
       - name: Inspect download
         run: tree ./${{ env.REPOTITLE_LOWERCASE }}
 
-      - name: Run the downloaded pipeline
+      - name: Run the downloaded pipeline (stub)
+        id: stub_run_pipeline
+        continue-on-error: true
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
+      - name: Run the downloaded pipeline (stub run not supported)
+        id: run_pipeline
+        if: ${{ job.steps.stub_run_pipeline.status == failure() }}
+        env:
+          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_HOME_MOUNT: true
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 41963bec..52217b84 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -4,7 +4,7 @@ on:
     types: [created]
 
 jobs:
-  deploy:
+  fix-linting:
     # Only run if comment is on a PR with the main repo, and if it contains the magic keywords
     if: >
       contains(github.event.comment.html_url, '/pull/') &&
@@ -13,10 +13,17 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
+      # indication that the linting is being fixed
+      - name: React on comment
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: eyes
+
       # Action runs on the issue comment, so we don't get the PR by default
       # Use the gh cli to check out the PR
       - name: Checkout Pull Request
@@ -24,25 +31,59 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      - name: Set up Python 3.11
-        uses: actions/setup-python@v5
+      # Install and run pre-commit
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
-          cache: "pip"
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
 
       - name: Run pre-commit
-        run: pre-commit run --all-files || echo "status=fail" >> $GITHUB_ENV
+        id: pre-commit
+        run: pre-commit run --all-files
+        continue-on-error: true
+
+      # indication that the linting has finished
+      - name: react if linting finished succesfully
+        if: steps.pre-commit.outcome == 'success'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: "+1"
 
       - name: Commit & push changes
-        if: env.status == 'fail'
+        id: commit-and-push
+        if: steps.pre-commit.outcome == 'failure'
         run: |
           git config user.email "core@nf-co.re"
           git config user.name "nf-core-bot"
           git config push.default upstream
           git add .
           git status
-          git commit -m "[automated] Fix linting with pre-commit"
+          git commit -m "[automated] Fix code linting"
           git push
+
+      - name: react if linting errors were fixed
+        id: react-if-fixed
+        if: steps.commit-and-push.outcome == 'success'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: hooray
+
+      - name: react if linting errors were not fixed
+        if: steps.commit-and-push.outcome == 'failure'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: confused
+
+      - name: react if linting errors were not fixed
+        if: steps.commit-and-push.outcome  == 'failure'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          issue-number: ${{ github.event.issue.number }}
+          body: |
+            @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually.
+            See [CI log](https://github.com/plant-food-research-open/assemblyqc/actions/runs/${{ github.run_id }}) for more details.
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index f807f5e2..a225c816 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,12 +14,12 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
-      - name: Set up Python 3.11
-        uses: actions/setup-python@v5
+      - name: Set up Python 3.12
+        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
+          python-version: "3.12"
           cache: "pip"
 
       - name: Install pre-commit
@@ -28,42 +28,42 @@ jobs:
       - name: Run pre-commit
         run: pre-commit run --all-files
 
-  # nf-core:
-  #   runs-on: ubuntu-latest
-  #   steps:
-  #     - name: Check out pipeline code
-  #       uses: actions/checkout@v4
+  nf-core:
+    runs-on: ubuntu-latest
+    steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
-  #     - name: Install Nextflow
-  #       uses: nf-core/setup-nextflow@v1
+      - name: Install Nextflow
+        uses: nf-core/setup-nextflow@v2
 
-  #     - uses: actions/setup-python@v5
-  #       with:
-  #         python-version: "3.11"
-  #         architecture: "x64"
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+        with:
+          python-version: "3.12"
+          architecture: "x64"
 
-  #     - name: Install dependencies
-  #       run: |
-  #         python -m pip install --upgrade pip
-  #         pip install nf-core
+      - name: Install dependencies
+        run: |
+          python -m pip install --upgrade pip
+          pip install nf-core
 
-  #     - name: Run nf-core lint
-  #       env:
-  #         GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
-  #         GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-  #         GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-  #       run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
+      - name: Run nf-core lint
+        env:
+          GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
+        run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
-  #     - name: Save PR number
-  #       if: ${{ always() }}
-  #       run: echo ${{ github.event.pull_request.number }} > PR_number.txt
+      - name: Save PR number
+        if: ${{ always() }}
+        run: echo ${{ github.event.pull_request.number }} > PR_number.txt
 
-  #     - name: Upload linting log file artifact
-  #       if: ${{ always() }}
-  #       uses: actions/upload-artifact@v4
-  #       with:
-  #         name: linting-logs
-  #         path: |
-  #           lint_log.txt
-  #           lint_results.md
-  #           PR_number.txt
+      - name: Upload linting log file artifact
+        if: ${{ always() }}
+        uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
+        with:
+          name: linting-logs
+          path: |
+            lint_log.txt
+            lint_results.md
+            PR_number.txt
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 147bcd10..40acc23f 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@v3
+        uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@v2
+        uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
new file mode 100644
index 00000000..03ecfcf7
--- /dev/null
+++ b/.github/workflows/release-announcements.yml
@@ -0,0 +1,75 @@
+name: release-announcements
+# Automatic release toot and tweet anouncements
+on:
+  release:
+    types: [published]
+  workflow_dispatch:
+
+jobs:
+  toot:
+    runs-on: ubuntu-latest
+    steps:
+      - name: get topics and convert to hashtags
+        id: get_topics
+        run: |
+          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')"  >> $GITHUB_OUTPUT
+
+      - uses: rzr/fediverse-action@master
+        with:
+          access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
+          host: "mstdn.science" # custom host if not "mastodon.social" (default)
+          # GitHub event payload
+          # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release
+          message: |
+            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
+
+            Please see the changelog: ${{ github.event.release.html_url }}
+
+            ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
+
+  send-tweet:
+    runs-on: ubuntu-latest
+
+    steps:
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+        with:
+          python-version: "3.10"
+      - name: Install dependencies
+        run: pip install tweepy==4.14.0
+      - name: Send tweet
+        shell: python
+        run: |
+          import os
+          import tweepy
+
+          client = tweepy.Client(
+              access_token=os.getenv("TWITTER_ACCESS_TOKEN"),
+              access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"),
+              consumer_key=os.getenv("TWITTER_CONSUMER_KEY"),
+              consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"),
+          )
+          tweet = os.getenv("TWEET")
+          client.create_tweet(text=tweet)
+        env:
+          TWEET: |
+            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
+
+            Please see the changelog: ${{ github.event.release.html_url }}
+          TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }}
+          TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }}
+          TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }}
+          TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }}
+
+  bsky-post:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0
+        with:
+          post: |
+            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
+
+            Please see the changelog: ${{ github.event.release.html_url }}
+        env:
+          BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }}
+          BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }}
+          #
diff --git a/.gitpod.yml b/.gitpod.yml
index 363d5b1d..105a1821 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -10,13 +10,11 @@ tasks:
 
 vscode:
   extensions: # based on nf-core.nf-core-extensionpack
-    - codezombiech.gitignore # Language support for .gitignore files
-    # - cssho.vscode-svgviewer                 # SVG viewer
     - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code
-    - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
     - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
     - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
     - mechatroner.rainbow-csv # Highlight columns in csv files in different colors
-    # - nextflow.nextflow                      # Nextflow syntax highlighting
+    # - nextflow.nextflow                    # Nextflow syntax highlighting
     - oderwat.indent-rainbow # Highlight indentation level
     - streetsidesoftware.code-spell-checker # Spelling checker for source code
+    - charliermarsh.ruff # Code linter Ruff
diff --git a/.nf-core.yml b/.nf-core.yml
index 61d19b4d..71f34ac4 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -18,6 +18,7 @@ lint:
   nextflow_config:
     - manifest.name
     - manifest.homePage
+nf_core_version: 2.13.2.dev0
 repository_type: pipeline
 template:
   prefix: plant-food-research-open
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index bc85d767..9b0780ba 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -3,6 +3,9 @@ repos:
     rev: "v3.1.0"
     hooks:
       - id: prettier
+        additional_dependencies:
+          - prettier@3.2.5
+
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
     rev: "2.7.3"
     hooks:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9a3aa939..5c8791d1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,6 +5,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## 2.0.0 - [22-April-2024]
 
+1. Updated nf-core/template to 2.13.2.dev0
+
 ### `Added`
 
 1. Updated modules and sub-workflows
diff --git a/README.md b/README.md
index ba0154c5..fa4fa1e5 100644
--- a/README.md
+++ b/README.md
@@ -1,11 +1,12 @@
-[![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20CI/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+CI%22)
-[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.10647870-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.10647870)
+[![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/ci.yml/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/ci.yml)
+[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/linting.yml/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/linting.yml)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.10647870-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.10647870)
+[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda ❌](http://img.shields.io/badge/run%20with-conda%20❌-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
-[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
+[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
 
 ## Introduction
 
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
new file mode 100644
index 00000000..759adecd
--- /dev/null
+++ b/assets/multiqc_config.yml
@@ -0,0 +1,16 @@
+report_comment: >
+  
+  This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/releases/tag/2.0.0" target="_blank">plant-food-research-open/assemblyqc</a>
+  analysis pipeline.
+  
+report_section_order:
+  "plant-food-research-open-assemblyqc-methods-description":
+    order: -1000
+  software_versions:
+    order: -1001
+  "plant-food-research-open-assemblyqc-summary":
+    order: -1002
+
+export_plots: true
+
+disable_version_detection: true
diff --git a/conf/base.config b/conf/base.config
index c7f00271..0e066d52 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -72,7 +72,4 @@ process {
     withName:DNADIFF {
         time   = { check_max( 7.day  * task.attempt, 'time'    ) }
     }
-    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
-        cache = false
-    }
 }
diff --git a/conf/modules.config b/conf/modules.config
index 0b1ba155..3185b2ad 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -12,14 +12,6 @@
 
 process {
 
-    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            pattern: '*_versions.yml'
-        ]
-    }
-
     withName: ASSEMBLATHON_STATS {
         publishDir = [
             path: { "${params.outdir}/assemblathon_stats" },
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
deleted file mode 100755
index 4ce590c0..00000000
--- a/lib/NfcoreTemplate.groovy
+++ /dev/null
@@ -1,351 +0,0 @@
-//
-// This file holds several functions used within the nf-core pipeline template.
-//
-
-import org.yaml.snakeyaml.Yaml
-import groovy.json.JsonOutput
-import nextflow.extension.FilesEx
-
-class NfcoreTemplate {
-
-    //
-    // Check AWS Batch related parameters have been specified correctly
-    //
-    public static void awsBatch(workflow, params) {
-        if (workflow.profile.contains('awsbatch')) {
-            // Check params.awsqueue and params.awsregion have been set if running on AWSBatch
-            assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
-            // Check outdir paths to be S3 buckets if running on AWSBatch
-            assert params.outdir.startsWith('s3:')       : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
-        }
-    }
-
-    //
-    //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
-    //
-    public static void checkConfigProvided(workflow, log) {
-        if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
-            log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
-                    "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
-                    "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
-                    "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
-                    "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
-                    "Please refer to the quick start section and usage docs for the pipeline.\n "
-        }
-    }
-
-    //
-    // Generate version string
-    //
-    public static String version(workflow) {
-        String version_string = ""
-
-        if (workflow.manifest.version) {
-            def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
-            version_string += "${prefix_v}${workflow.manifest.version}"
-        }
-
-        if (workflow.commitId) {
-            def git_shortsha = workflow.commitId.substring(0, 7)
-            version_string += "-g${git_shortsha}"
-        }
-
-        return version_string
-    }
-
-    //
-    // Construct and send completion email
-    //
-    public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) {
-
-        // Set up the e-mail variables
-        def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
-        if (!workflow.success) {
-            subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
-        }
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['Date Started']              = workflow.start
-        misc_fields['Date Completed']            = workflow.complete
-        misc_fields['Pipeline script file path'] = workflow.scriptFile
-        misc_fields['Pipeline script hash ID']   = workflow.scriptId
-        if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
-        if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
-        if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
-        misc_fields['Nextflow Version']           = workflow.nextflow.version
-        misc_fields['Nextflow Build']             = workflow.nextflow.build
-        misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
-
-        def email_fields = [:]
-        email_fields['version']      = NfcoreTemplate.version(workflow)
-        email_fields['runName']      = workflow.runName
-        email_fields['success']      = workflow.success
-        email_fields['dateComplete'] = workflow.complete
-        email_fields['duration']     = workflow.duration
-        email_fields['exitStatus']   = workflow.exitStatus
-        email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        email_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        email_fields['commandLine']  = workflow.commandLine
-        email_fields['projectDir']   = workflow.projectDir
-        email_fields['summary']      = summary << misc_fields
-
-        // On success try attach the multiqc report
-        def mqc_report = null
-        try {
-            if (workflow.success) {
-                mqc_report = multiqc_report.getVal()
-                if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
-                    if (mqc_report.size() > 1) {
-                        log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
-                    }
-                    mqc_report = mqc_report[0]
-                }
-            }
-        } catch (all) {
-            if (multiqc_report) {
-                log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
-            }
-        }
-
-        // Check if we are only sending emails on failure
-        def email_address = params.email
-        if (!params.email && params.email_on_fail && !workflow.success) {
-            email_address = params.email_on_fail
-        }
-
-        // Render the TXT template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        def tf           = new File("$projectDir/assets/email_template.txt")
-        def txt_template = engine.createTemplate(tf).make(email_fields)
-        def email_txt    = txt_template.toString()
-
-        // Render the HTML template
-        def hf            = new File("$projectDir/assets/email_template.html")
-        def html_template = engine.createTemplate(hf).make(email_fields)
-        def email_html    = html_template.toString()
-
-        // Render the sendmail template
-        def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
-        def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
-        def sf                     = new File("$projectDir/assets/sendmail_template.txt")
-        def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
-        def sendmail_html          = sendmail_template.toString()
-
-        // Send the HTML e-mail
-        Map colors = logColours(params.monochrome_logs)
-        if (email_address) {
-            try {
-                if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
-                // Try to send HTML e-mail using sendmail
-                def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
-                sendmail_tf.withWriter { w -> w << sendmail_html }
-                [ 'sendmail', '-t' ].execute() << sendmail_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
-            } catch (all) {
-                // Catch failures and try with plaintext
-                def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-                if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
-                    mail_cmd += [ '-A', mqc_report ]
-                }
-                mail_cmd.execute() << email_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
-            }
-        }
-
-        // Write summary e-mail HTML to a file
-        def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
-        output_hf.withWriter { w -> w << email_html }
-        FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html");
-        output_hf.delete()
-
-        // Write summary e-mail TXT to a file
-        def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
-        output_tf.withWriter { w -> w << email_txt }
-        FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt");
-        output_tf.delete()
-    }
-
-    //
-    // Construct and send a notification to a web server as JSON
-    // e.g. Microsoft Teams and Slack
-    //
-    public static void IM_notification(workflow, params, summary_params, projectDir, log) {
-        def hook_url = params.hook_url
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['start']                                = workflow.start
-        misc_fields['complete']                             = workflow.complete
-        misc_fields['scriptfile']                           = workflow.scriptFile
-        misc_fields['scriptid']                             = workflow.scriptId
-        if (workflow.repository) misc_fields['repository']  = workflow.repository
-        if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
-        if (workflow.revision)   misc_fields['revision']    = workflow.revision
-        misc_fields['nxf_version']                          = workflow.nextflow.version
-        misc_fields['nxf_build']                            = workflow.nextflow.build
-        misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
-
-        def msg_fields = [:]
-        msg_fields['version']      = NfcoreTemplate.version(workflow)
-        msg_fields['runName']      = workflow.runName
-        msg_fields['success']      = workflow.success
-        msg_fields['dateComplete'] = workflow.complete
-        msg_fields['duration']     = workflow.duration
-        msg_fields['exitStatus']   = workflow.exitStatus
-        msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
-        msg_fields['projectDir']   = workflow.projectDir
-        msg_fields['summary']      = summary << misc_fields
-
-        // Render the JSON template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        // Different JSON depending on the service provider
-        // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
-        def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
-        def hf            = new File("$projectDir/assets/${json_path}")
-        def json_template = engine.createTemplate(hf).make(msg_fields)
-        def json_message  = json_template.toString()
-
-        // POST
-        def post = new URL(hook_url).openConnection();
-        post.setRequestMethod("POST")
-        post.setDoOutput(true)
-        post.setRequestProperty("Content-Type", "application/json")
-        post.getOutputStream().write(json_message.getBytes("UTF-8"));
-        def postRC = post.getResponseCode();
-        if (! postRC.equals(200)) {
-            log.warn(post.getErrorStream().getText());
-        }
-    }
-
-    //
-    // Dump pipeline parameters in a json file
-    //
-    public static void dump_parameters(workflow, params) {
-        def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-        def filename   = "params_${timestamp}.json"
-        def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
-        def jsonStr    = JsonOutput.toJson(params)
-        temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
-
-        FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json")
-        temp_pf.delete()
-    }
-
-    //
-    // Print pipeline summary on completion
-    //
-    public static void summary(workflow, params, log) {
-        Map colors = logColours(params.monochrome_logs)
-        if (workflow.success) {
-            if (workflow.stats.ignoredCount == 0) {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
-            } else {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
-            }
-        } else {
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
-        }
-    }
-
-    //
-    // ANSII Colours used for terminal logging
-    //
-    public static Map logColours(Boolean monochrome_logs) {
-        Map colorcodes = [:]
-
-        // Reset / Meta
-        colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
-        colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
-        colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
-        colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
-        colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
-        colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
-        colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
-
-        // Regular Colors
-        colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
-        colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
-        colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
-        colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
-        colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
-        colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
-        colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
-        colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
-
-        // Bold
-        colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
-        colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
-        colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
-        colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
-        colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
-        colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
-        colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
-        colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
-
-        // Underline
-        colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
-        colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
-        colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
-        colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
-        colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
-        colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
-        colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
-        colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
-
-        // High Intensity
-        colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
-        colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
-        colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
-        colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
-        colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
-        colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
-        colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
-        colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
-
-        // Bold High Intensity
-        colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
-        colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
-        colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
-        colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
-        colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
-        colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
-        colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
-        colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
-
-        return colorcodes
-    }
-
-    //
-    // Does what is says on the tin
-    //
-    public static String dashedLine(monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-    }
-
-    //
-    // nf-core logo
-    //
-    public static String logo(workflow, monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        String workflow_version = NfcoreTemplate.version(workflow)
-        String.format(
-            """\n
-            ${dashedLine(monochrome_logs)}
-            ${colors.purple}  ${workflow.manifest.name} ${workflow_version}${colors.reset}
-            ${dashedLine(monochrome_logs)}
-            """.stripIndent()
-        )
-    }
-}
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
deleted file mode 100644
index 8d030f4e..00000000
--- a/lib/Utils.groovy
+++ /dev/null
@@ -1,47 +0,0 @@
-//
-// This file holds several Groovy functions that could be useful for any Nextflow pipeline
-//
-
-import org.yaml.snakeyaml.Yaml
-
-class Utils {
-
-    //
-    // When running with -profile conda, warn if channels have not been set-up appropriately
-    //
-    public static void checkCondaChannels(log) {
-        Yaml parser = new Yaml()
-        def channels = []
-        try {
-            def config = parser.load("conda config --show channels".execute().text)
-            channels = config.channels
-        } catch(NullPointerException | IOException e) {
-            log.warn "Could not verify conda channel configuration."
-            return
-        }
-
-        // Check that all channels are present
-        // This channel list is ordered by required channel priority.
-        def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
-        def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
-
-        // Check that they are in the right order
-        def channel_priority_violation = false
-        def n = required_channels_in_order.size()
-        for (int i = 0; i < n - 1; i++) {
-            channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
-        }
-
-        if (channels_missing | channel_priority_violation) {
-            log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  There is a problem with your Conda configuration!\n\n" +
-                "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
-                "  Please refer to https://bioconda.github.io/\n" +
-                "  The observed channel order is \n" +
-                "  ${channels}\n" +
-                "  but the following channel order is required:\n" +
-                "  ${required_channels_in_order}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-        }
-    }
-}
diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy
deleted file mode 100755
index 5ec2cc32..00000000
--- a/lib/WorkflowAssemblyqc.groovy
+++ /dev/null
@@ -1,100 +0,0 @@
-//
-// This file holds several functions specific to the workflow/assemblyqc.nf in the plant-food-research-open/assemblyqc pipeline
-//
-
-import nextflow.Nextflow
-import groovy.text.SimpleTemplateEngine
-import groovy.json.JsonOutput
-
-class WorkflowAssemblyqc {
-
-    //
-    // Check and validate parameters
-    //
-    public static void initialise(params, log) {
-        // Check for ncbi_fcs_adaptor_empire
-        if (!params.ncbi_fcs_adaptor_skip && !params.ncbi_fcs_adaptor_empire) {
-            Nextflow.error('ncbi_fcs_adaptor_empire must be provided when executing NCBI FCS Adaptor')
-        }
-
-        // Check for ncbi_fcs_gx_tax_id
-        if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) {
-            Nextflow.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX')
-        }
-
-        // Check for ncbi_fcs_gx_db_path
-        if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) {
-            Nextflow.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX')
-        }
-
-        // Check for busco_mode
-        if (!params.busco_skip && !params.busco_mode) {
-            Nextflow.error("busco_mode must be provided when executing BUSCO")
-        }
-
-        // Check for busco_lineage_datasets
-        if (!params.busco_skip && !params.busco_lineage_datasets) {
-            Nextflow.error('busco_lineage_datasets must be provided when executing BUSCO')
-        }
-
-        // Check for tidk_repeat_seq
-        if (!params.tidk_skip && !params.tidk_repeat_seq) {
-            Nextflow.error('tidk_repeat_seq must be provided when executing TIDK')
-        }
-
-        // Check for kraken2_db_path
-        if (!params.kraken2_skip && !params.kraken2_db_path) {
-            Nextflow.error('kraken2_db_path must be provided when executing Kraken2')
-        }
-    }
-
-    public static ArrayList validateInput(input) {
-        def inputFields = 5
-        def assemblyTags = input[(0..input.size()-1).step(inputFields)]
-
-        def tagCounts = [:]
-        assemblyTags.each { tag ->
-            tagCounts[tag] = tagCounts.containsKey(tag) ? tagCounts[tag] + 1 : 1
-        }
-        def repeatedTags = tagCounts.findAll { key, count -> count > 1 }.collect { key, count -> key }
-
-        if (repeatedTags.size() > 0) {
-            Nextflow.error("Please check input assemblysheet -> Multiple assemblies have the same tags!: ${repeatedTags}")
-        }
-
-        return input
-    }
-
-    public static ArrayList validateXrefAssemblies(xref) {
-        def xrefFields = 3
-        def xrefTags = xref[(0..xref.size()-1).step(xrefFields)]
-
-        def tagCounts = [:]
-        xrefTags.each { tag ->
-            tagCounts[tag] = tagCounts.containsKey(tag) ? tagCounts[tag] + 1 : 1
-        }
-        def repeatedTags = tagCounts.findAll { key, count -> count > 1 }.collect { key, count -> key }
-
-        if (repeatedTags.size() > 0) {
-            Nextflow.error("Please check synteny_xref_assemblies -> Multiple xref assemblies have the same tags!: ${repeatedTags}")
-        }
-
-        return xref
-    }
-
-    public static String jsonifyParams(params) {
-        return JsonOutput.toJson(params).toString()
-    }
-
-    public static String jsonifySummaryParams(params) {
-
-        def summary = [:]
-        for (group in params.keySet()) {
-            for (subgroup in params[group].keySet()) {
-                if ( params[group][subgroup] ) { summary << [ "$subgroup": "${params[group][subgroup]}" ] }
-            }
-        }
-
-        return JsonOutput.toJson(summary).toString()
-    }
-}
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
deleted file mode 100755
index abfc7acf..00000000
--- a/lib/WorkflowMain.groovy
+++ /dev/null
@@ -1,57 +0,0 @@
-//
-// This file holds several functions specific to the main.nf workflow in the plant-food-research-open/assemblyqc pipeline
-//
-
-import nextflow.Nextflow
-
-class WorkflowMain {
-
-    //
-    // Citation string for pipeline
-    //
-    public static String citation(workflow) {
-        return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-            "* The pipeline\n" +
-            "  https://doi.org/10.5281/zenodo.10647870\n\n" +
-            "* The nf-core framework\n" +
-            "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
-            "* Software dependencies\n" +
-            "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
-    }
-
-
-    //
-    // Validate parameters and print summary to screen
-    //
-    public static void initialise(workflow, params, log) {
-
-        // Print workflow version and exit on --version
-        if (params.version) {
-            String workflow_version = NfcoreTemplate.version(workflow)
-            log.info "${workflow.manifest.name} ${workflow_version}"
-            System.exit(0)
-        }
-
-        // Check that a -profile or Nextflow config has been provided to run the pipeline
-        NfcoreTemplate.checkConfigProvided(workflow, log)
-
-        // Check that conda channels are set-up correctly
-        if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-            Utils.checkCondaChannels(log)
-        }
-
-        // Check AWS batch settings
-        NfcoreTemplate.awsBatch(workflow, params)
-    }
-    //
-    // Get attribute from genome config file e.g. fasta
-    //
-    public static Object getGenomeAttribute(params, attribute) {
-        if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
-            if (params.genomes[ params.genome ].containsKey(attribute)) {
-                return params.genomes[ params.genome ][ attribute ]
-            }
-        }
-        return null
-    }
-}
diff --git a/main.nf b/main.nf
index 34f2ebb3..7a002146 100755
--- a/main.nf
+++ b/main.nf
@@ -11,55 +11,70 @@ nextflow.enable.dsl = 2
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    VALIDATE & PRINT PARAMETER SUMMARY
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { validateParameters; paramsHelp } from 'plugin/nf-validation'
-
-// Print help message if needed
-if (params.help) {
-    def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-    def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-    def String command = "nextflow run ${workflow.manifest.name} --input assemblysheet.csv --outdir ./results -profile docker"
-    log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs)
-    System.exit(0)
-}
-
-WorkflowMain.initialise(workflow, params, log)
-
-// Validate input parameters
-if (params.validate_params) {
-    validateParameters()
-}
+include { ASSEMBLYQC                } from './workflows/assemblyqc'
+include { PIPELINE_INITIALISATION   } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline'
+include { PIPELINE_COMPLETION       } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    NAMED WORKFLOW FOR PIPELINE
+    NAMED WORKFLOWS FOR PIPELINE
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { ASSEMBLYQC } from './workflows/assemblyqc'
-
 //
-// WORKFLOW: Run main plant-food-research-open/assemblyqc analysis pipeline
+// WORKFLOW: Run main analysis pipeline depending on type of input
 //
 workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
+
+    //
+    // WORKFLOW: Run pipeline
+    //
     ASSEMBLYQC ()
-}
 
+}
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    RUN ALL WORKFLOWS
+    RUN MAIN WORKFLOW
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-//
-// WORKFLOW: Execute a single named workflow for the pipeline
-// See: https://github.com/nf-core/rnaseq/issues/619
-//
 workflow {
+
+    main:
+
+    //
+    // SUBWORKFLOW: Run initialisation tasks
+    //
+    PIPELINE_INITIALISATION (
+        params.version,
+        params.help,
+        params.validate_params,
+        params.monochrome_logs,
+        args,
+        params.outdir,
+        params.input
+    )
+
+    //
+    // WORKFLOW: Run main workflow
+    //
     PLANTFOODRESEARCHOPEN_ASSEMBLYQC ()
+
+    //
+    // SUBWORKFLOW: Run completion tasks
+    //
+    PIPELINE_COMPLETION (
+        params.email,
+        params.email_on_fail,
+        params.plaintext_email,
+        params.outdir,
+        params.monochrome_logs,
+        params.hook_url
+    )
 }
 
 /*
diff --git a/modules.json b/modules.json
index d817ded9..564e7abd 100644
--- a/modules.json
+++ b/modules.json
@@ -8,72 +8,100 @@
                     "bwa/index": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fastq_bwa_mem_samblaster"]
+                        "installed_by": [
+                            "fastq_bwa_mem_samblaster"
+                        ]
                     },
                     "bwa/mem": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fastq_bwa_mem_samblaster"]
+                        "installed_by": [
+                            "fastq_bwa_mem_samblaster"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "custom/checkgff3fastacorrespondence": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["gff3_validate"]
+                        "installed_by": [
+                            "gff3_validate"
+                        ]
                     },
                     "custom/restoregffids": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "custom/shortenfastaids": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "gt/gff3": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["gff3_validate"]
+                        "installed_by": [
+                            "gff3_validate"
+                        ]
                     },
                     "gt/gff3validator": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["gff3_validate"]
+                        "installed_by": [
+                            "gff3_validate"
+                        ]
                     },
                     "gt/stat": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ltrfinder": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "ltrharvest": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "ltrretriever/lai": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "ltrretriever/ltrretriever": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fasta_ltrretriever_lai"]
+                        "installed_by": [
+                            "fasta_ltrretriever_lai"
+                        ]
                     },
                     "samblaster": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["fastq_bwa_mem_samblaster"]
+                        "installed_by": [
+                            "fastq_bwa_mem_samblaster"
+                        ]
                     }
                 }
             },
@@ -82,17 +110,23 @@
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "fastq_bwa_mem_samblaster": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "gff3_validate": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
@@ -103,57 +137,79 @@
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastavalidator": {
                         "branch": "master",
                         "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
-                        "installed_by": ["fastq_trim_fastp_fastqc"]
+                        "installed_by": [
+                            "fastq_trim_fastp_fastqc"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
-                        "installed_by": ["fastq_trim_fastp_fastqc"]
+                        "installed_by": [
+                            "fastq_trim_fastp_fastqc"
+                        ]
                     },
                     "gunzip": {
                         "branch": "master",
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "seqkit/seq": {
                         "branch": "master",
                         "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4",
-                        "installed_by": ["fasta_explore_search_plot_tidk"]
+                        "installed_by": [
+                            "fasta_explore_search_plot_tidk"
+                        ]
                     },
                     "seqkit/sort": {
                         "branch": "master",
                         "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d",
-                        "installed_by": ["fasta_explore_search_plot_tidk"]
+                        "installed_by": [
+                            "fasta_explore_search_plot_tidk"
+                        ]
                     },
                     "tidk/explore": {
                         "branch": "master",
                         "git_sha": "fad335b31b32d78f89a8340373d3ccfc0f2b18b5",
-                        "installed_by": ["fasta_explore_search_plot_tidk"]
+                        "installed_by": [
+                            "fasta_explore_search_plot_tidk"
+                        ]
                     },
                     "tidk/plot": {
                         "branch": "master",
                         "git_sha": "d54cd1d823f151854825da8ffe7289bcbf77206b",
-                        "installed_by": ["fasta_explore_search_plot_tidk"]
+                        "installed_by": [
+                            "fasta_explore_search_plot_tidk"
+                        ]
                     },
                     "tidk/search": {
                         "branch": "master",
                         "git_sha": "669a329f4aa37f5b7f03776c2ed1cd0ef122c626",
-                        "installed_by": ["fasta_explore_search_plot_tidk"]
+                        "installed_by": [
+                            "fasta_explore_search_plot_tidk"
+                        ]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -162,12 +218,34 @@
                     "fasta_explore_search_plot_tidk": {
                         "branch": "master",
                         "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "fastq_trim_fastp_fastqc": {
                         "branch": "master",
                         "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
-                        "installed_by": ["subworkflows"]
+                    },
+                    "utils_nextflow_pipeline": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": [
+                            "subworkflows"
+                        ]
+                    },
+                    "utils_nfcore_pipeline": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": [
+                            "subworkflows"
+                        ]
+                    },
+                    "utils_nfvalidation_plugin": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
deleted file mode 100644
index b48ced26..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: custom_dumpsoftwareversions
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::multiqc=1.20
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
deleted file mode 100644
index 105f9265..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ /dev/null
@@ -1,24 +0,0 @@
-process CUSTOM_DUMPSOFTWAREVERSIONS {
-    label 'process_single'
-
-    // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.20--pyhdfd78af_0' }"
-
-    input:
-    path versions
-
-    output:
-    path "software_versions.yml"    , emit: yml
-    path "software_versions_mqc.yml", emit: mqc_yml
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    template 'dumpsoftwareversions.py'
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
deleted file mode 100644
index 5f15a5fd..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ /dev/null
@@ -1,37 +0,0 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
-name: custom_dumpsoftwareversions
-description: Custom module used to dump software versions within the nf-core pipeline template
-keywords:
-  - custom
-  - dump
-  - version
-tools:
-  - custom:
-      description: Custom module used to dump software versions within the nf-core pipeline template
-      homepage: https://github.com/nf-core/tools
-      documentation: https://github.com/nf-core/tools
-      licence: ["MIT"]
-input:
-  - versions:
-      type: file
-      description: YML file containing software versions
-      pattern: "*.yml"
-output:
-  - yml:
-      type: file
-      description: Standard YML file containing software versions
-      pattern: "software_versions.yml"
-  - mqc_yml:
-      type: file
-      description: MultiQC custom content YML file containing software versions
-      pattern: "software_versions_mqc.yml"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@grst"
-maintainers:
-  - "@drpatelh"
-  - "@grst"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
deleted file mode 100755
index da033408..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ /dev/null
@@ -1,101 +0,0 @@
-#!/usr/bin/env python
-
-
-"""Provide functions to merge multiple versions.yml files."""
-
-
-import yaml
-import platform
-from textwrap import dedent
-
-
-def _make_versions_html(versions):
-    """Generate a tabular HTML output of all versions for MultiQC."""
-    html = [
-        dedent(
-            """\\
-            <style>
-            #nf-core-versions tbody:nth-child(even) {
-                background-color: #f2f2f2;
-            }
-            </style>
-            <table class="table" style="width:100%" id="nf-core-versions">
-                <thead>
-                    <tr>
-                        <th> Process Name </th>
-                        <th> Software </th>
-                        <th> Version  </th>
-                    </tr>
-                </thead>
-            """
-        )
-    ]
-    for process, tmp_versions in sorted(versions.items()):
-        html.append("<tbody>")
-        for i, (tool, version) in enumerate(sorted(tmp_versions.items())):
-            html.append(
-                dedent(
-                    f"""\\
-                    <tr>
-                        <td><samp>{process if (i == 0) else ''}</samp></td>
-                        <td><samp>{tool}</samp></td>
-                        <td><samp>{version}</samp></td>
-                    </tr>
-                    """
-                )
-            )
-        html.append("</tbody>")
-    html.append("</table>")
-    return "\\n".join(html)
-
-
-def main():
-    """Load all version files and generate merged output."""
-    versions_this_module = {}
-    versions_this_module["${task.process}"] = {
-        "python": platform.python_version(),
-        "yaml": yaml.__version__,
-    }
-
-    with open("$versions") as f:
-        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
-
-    # aggregate versions by the module name (derived from fully-qualified process name)
-    versions_by_module = {}
-    for process, process_versions in versions_by_process.items():
-        module = process.split(":")[-1]
-        try:
-            if versions_by_module[module] != process_versions:
-                raise AssertionError(
-                    "We assume that software versions are the same between all modules. "
-                    "If you see this error-message it means you discovered an edge-case "
-                    "and should open an issue in nf-core/tools. "
-                )
-        except KeyError:
-            versions_by_module[module] = process_versions
-
-    versions_by_module["Workflow"] = {
-        "Nextflow": "$workflow.nextflow.version",
-        "$workflow.manifest.name": "$workflow.manifest.version",
-    }
-
-    versions_mqc = {
-        "id": "software_versions",
-        "section_name": "${workflow.manifest.name} Software Versions",
-        "section_href": "https://github.com/${workflow.manifest.name}",
-        "plot_type": "html",
-        "description": "are collected at run time from the software output.",
-        "data": _make_versions_html(versions_by_module),
-    }
-
-    with open("software_versions.yml", "w") as f:
-        yaml.dump(versions_by_module, f, default_flow_style=False)
-    with open("software_versions_mqc.yml", "w") as f:
-        yaml.dump(versions_mqc, f, default_flow_style=False)
-
-    with open("versions.yml", "w") as f:
-        yaml.dump(versions_this_module, f, default_flow_style=False)
-
-
-if __name__ == "__main__":
-    main()
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
deleted file mode 100644
index b1e1630b..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
+++ /dev/null
@@ -1,43 +0,0 @@
-nextflow_process {
-
-    name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS"
-    script "../main.nf"
-    process "CUSTOM_DUMPSOFTWAREVERSIONS"
-    tag "modules"
-    tag "modules_nfcore"
-    tag "custom"
-    tag "dumpsoftwareversions"
-    tag "custom/dumpsoftwareversions"
-
-    test("Should run without failures") {
-        when {
-            process {
-                """
-                def tool1_version = '''
-                TOOL1:
-                    tool1: 0.11.9
-                '''.stripIndent()
-
-                def tool2_version = '''
-                TOOL2:
-                    tool2: 1.9
-                '''.stripIndent()
-
-                input[0] = Channel.of(tool1_version, tool2_version).collectFile()
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    file(process.out.mqc_yml[0]).readLines()[0..10],
-                    file(process.out.yml[0]).readLines()[0..7]
-                    ).match()
-                }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
deleted file mode 100644
index 5f59a936..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
+++ /dev/null
@@ -1,33 +0,0 @@
-{
-    "Should run without failures": {
-        "content": [
-            [
-                "versions.yml:md5,76d454d92244589d32455833f7c1ba6d"
-            ],
-            [
-                "data: \"<style>\\n#nf-core-versions tbody:nth-child(even) {\\n    background-color: #f2f2f2;\\n\\",
-                "  }\\n</style>\\n<table class=\\\"table\\\" style=\\\"width:100%\\\" id=\\\"nf-core-versions\\\"\\",
-                "  >\\n    <thead>\\n        <tr>\\n            <th> Process Name </th>\\n            <th>\\",
-                "  \\ Software </th>\\n            <th> Version  </th>\\n        </tr>\\n    </thead>\\n\\",
-                "  \\n<tbody>\\n<tr>\\n    <td><samp>CUSTOM_DUMPSOFTWAREVERSIONS</samp></td>\\n    <td><samp>python</samp></td>\\n\\",
-                "  \\    <td><samp>3.11.7</samp></td>\\n</tr>\\n\\n<tr>\\n    <td><samp></samp></td>\\n \\",
-                "  \\   <td><samp>yaml</samp></td>\\n    <td><samp>5.4.1</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
-                "  <tbody>\\n<tr>\\n    <td><samp>TOOL1</samp></td>\\n    <td><samp>tool1</samp></td>\\n\\",
-                "  \\    <td><samp>0.11.9</samp></td>\\n</tr>\\n\\n</tbody>\\n<tbody>\\n<tr>\\n    <td><samp>TOOL2</samp></td>\\n\\",
-                "  \\    <td><samp>tool2</samp></td>\\n    <td><samp>1.9</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
-                "  <tbody>\\n<tr>\\n    <td><samp>Workflow</samp></td>\\n    <td><samp>Nextflow</samp></td>\\n\\"
-            ],
-            [
-                "CUSTOM_DUMPSOFTWAREVERSIONS:",
-                "  python: 3.11.7",
-                "  yaml: 5.4.1",
-                "TOOL1:",
-                "  tool1: 0.11.9",
-                "TOOL2:",
-                "  tool2: '1.9'",
-                "Workflow:"
-            ]
-        ],
-        "timestamp": "2024-01-09T23:01:18.710682"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
deleted file mode 100644
index 405aa24a..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-custom/dumpsoftwareversions:
-  - modules/nf-core/custom/dumpsoftwareversions/**
diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml
index 25910b34..3c723b21 100644
--- a/modules/nf-core/gunzip/environment.yml
+++ b/modules/nf-core/gunzip/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - conda-forge::sed=4.7
+  - bioconda::multiqc=1.21
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
new file mode 100644
index 00000000..47ac352f
--- /dev/null
+++ b/modules/nf-core/multiqc/main.nf
@@ -0,0 +1,55 @@
+process MULTIQC {
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.21--pyhdfd78af_0' }"
+
+    input:
+    path  multiqc_files, stageAs: "?/*"
+    path(multiqc_config)
+    path(extra_multiqc_config)
+    path(multiqc_logo)
+
+    output:
+    path "*multiqc_report.html", emit: report
+    path "*_data"              , emit: data
+    path "*_plots"             , optional:true, emit: plots
+    path "versions.yml"        , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def config = multiqc_config ? "--config $multiqc_config" : ''
+    def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
+    def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : ''
+    """
+    multiqc \\
+        --force \\
+        $args \\
+        $config \\
+        $extra_config \\
+        $logo \\
+        .
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    mkdir multiqc_data
+    touch multiqc_plots
+    touch multiqc_report.html
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
new file mode 100644
index 00000000..45a9bc35
--- /dev/null
+++ b/modules/nf-core/multiqc/meta.yml
@@ -0,0 +1,58 @@
+name: multiqc
+description: Aggregate results from bioinformatics analyses across many samples into a single report
+keywords:
+  - QC
+  - bioinformatics tools
+  - Beautiful stand-alone HTML report
+tools:
+  - multiqc:
+      description: |
+        MultiQC searches a given directory for analysis logs and compiles a HTML report.
+        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
+      homepage: https://multiqc.info/
+      documentation: https://multiqc.info/docs/
+      licence: ["GPL-3.0-or-later"]
+input:
+  - multiqc_files:
+      type: file
+      description: |
+        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - multiqc_config:
+      type: file
+      description: Optional config yml for MultiQC
+      pattern: "*.{yml,yaml}"
+  - extra_multiqc_config:
+      type: file
+      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
+      pattern: "*.{yml,yaml}"
+  - multiqc_logo:
+      type: file
+      description: Optional logo file for MultiQC
+      pattern: "*.{png}"
+output:
+  - report:
+      type: file
+      description: MultiQC report file
+      pattern: "multiqc_report.html"
+  - data:
+      type: directory
+      description: MultiQC data dir
+      pattern: "multiqc_data"
+  - plots:
+      type: file
+      description: Plots created by MultiQC
+      pattern: "*_data"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"
+  - "@jfy133"
+maintainers:
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"
+  - "@jfy133"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
new file mode 100644
index 00000000..f1c4242e
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -0,0 +1,84 @@
+nextflow_process {
+
+    name "Test Process MULTIQC"
+    script "../main.nf"
+    process "MULTIQC"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "multiqc"
+
+    test("sarscov2 single-end [fastqc]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("multiqc_versions_single") }
+            )
+        }
+
+    }
+
+    test("sarscov2 single-end [fastqc] [config]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("multiqc_versions_config") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastqc] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.report.collect { file(it).getName() } +
+                                process.out.data.collect { file(it).getName() } +
+                                process.out.plots.collect { file(it).getName() } +
+                                process.out.versions ).match("multiqc_stub") }
+            )
+        }
+
+    }
+}
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..bfebd802
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -0,0 +1,41 @@
+{
+    "multiqc_versions_single": {
+        "content": [
+            [
+                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-29T08:48:55.657331"
+    },
+    "multiqc_stub": {
+        "content": [
+            [
+                "multiqc_report.html",
+                "multiqc_data",
+                "multiqc_plots",
+                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-29T08:49:49.071937"
+    },
+    "multiqc_versions_config": {
+        "content": [
+            [
+                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-29T08:49:25.457567"
+    }
+}
\ No newline at end of file
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f16a18ba..6f4e61ed 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -14,8 +14,10 @@
                 "input": {
                     "type": "string",
                     "format": "file-path",
-                    "mimetype": "csv",
+                    "exists": true,
                     "schema": "assets/schema_input.json",
+                    "mimetype": "text/csv",
+                    "pattern": "^\\S+\\.csv$",
                     "help_text": "FASTA and other associated files for input assemblies provided as a formatted CSV file",
                     "description": "Input assembly sheet in CSV format",
                     "fa_icon": "fas fa-file-csv"
diff --git a/pyproject.toml b/pyproject.toml
deleted file mode 100644
index 7d08e1c8..00000000
--- a/pyproject.toml
+++ /dev/null
@@ -1,13 +0,0 @@
-# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff.
-# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
-[tool.ruff]
-line-length = 120
-target-version = "py38"
-select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
-cache-dir = "~/.cache/ruff"
-
-[tool.ruff.isort]
-known-first-party = ["nf_core"]
-
-[tool.ruff.per-file-ignores]
-"__init__.py" = ["E402", "F401"]
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
new file mode 100644
index 00000000..7013d07c
--- /dev/null
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -0,0 +1,219 @@
+//
+// Subworkflow with functionality specific to the plant-food-research-open/assemblyqc pipeline
+//
+
+import groovy.json.JsonOutput
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin'
+include { paramsSummaryMap          } from 'plugin/nf-validation'
+include { fromSamplesheet           } from 'plugin/nf-validation'
+include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
+include { completionEmail           } from '../../nf-core/utils_nfcore_pipeline'
+include { completionSummary         } from '../../nf-core/utils_nfcore_pipeline'
+include { dashedLine                } from '../../nf-core/utils_nfcore_pipeline'
+include { nfCoreLogo                } from '../../nf-core/utils_nfcore_pipeline'
+include { imNotification            } from '../../nf-core/utils_nfcore_pipeline'
+include { UTILS_NFCORE_PIPELINE     } from '../../nf-core/utils_nfcore_pipeline'
+include { workflowCitation          } from '../../nf-core/utils_nfcore_pipeline'
+
+/*
+========================================================================================
+    SUBWORKFLOW TO INITIALISE PIPELINE
+========================================================================================
+*/
+
+workflow PIPELINE_INITIALISATION {
+
+    take:
+    version           // boolean: Display version and exit
+    help              // boolean: Display help text
+    validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
+    monochrome_logs   // boolean: Do not use coloured log outputs
+    nextflow_cli_args //   array: List of positional nextflow CLI args
+    outdir            //  string: The output directory where the results will be saved
+    input             //  string: Path to input assemblysheet
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    //
+    // Print version and exit if required and dump pipeline parameters to JSON file
+    //
+    UTILS_NEXTFLOW_PIPELINE (
+        version,
+        true,
+        outdir,
+        workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1
+    )
+
+    //
+    // Validate parameters and generate parameter summary to stdout
+    //
+    pre_help_text = nfCoreLogo(monochrome_logs)
+    post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs)
+    def String workflow_command = "nextflow run ${workflow.manifest.name} -profile <docker/singularity/.../institute> --input assemblysheet.csv --outdir <OUTDIR>"
+    UTILS_NFVALIDATION_PLUGIN (
+        help,
+        workflow_command,
+        pre_help_text,
+        post_help_text,
+        validate_params,
+        "nextflow_schema.json"
+    )
+
+    //
+    // Check config provided to the pipeline
+    //
+    UTILS_NFCORE_PIPELINE (
+        nextflow_cli_args
+    )
+    //
+    // Custom validation for pipeline parameters
+    //
+    validateInputParameters()
+
+    emit:
+    versions    = ch_versions
+}
+
+/*
+========================================================================================
+    SUBWORKFLOW FOR PIPELINE COMPLETION
+========================================================================================
+*/
+
+workflow PIPELINE_COMPLETION {
+
+    take:
+    email           //  string: email address
+    email_on_fail   //  string: email address sent on pipeline failure
+    plaintext_email // boolean: Send plain-text email instead of HTML
+    outdir          //    path: Path to output directory where results will be published
+    monochrome_logs // boolean: Disable ANSI colour codes in log output
+    hook_url        //  string: hook URL for notifications
+
+    main:
+
+    summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+
+    //
+    // Completion email and summary
+    //
+    workflow.onComplete {
+        if (email || email_on_fail) {
+            completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs)
+        }
+
+        completionSummary(monochrome_logs)
+
+        if (hook_url) {
+            imNotification(summary_params, hook_url)
+        }
+    }
+
+    workflow.onError {
+        log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting"
+    }
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+//
+// Check and validate pipeline parameters
+//
+def validateInputParameters() {
+    // Check for ncbi_fcs_adaptor_empire
+    if (!params.ncbi_fcs_adaptor_skip && !params.ncbi_fcs_adaptor_empire) {
+        error('ncbi_fcs_adaptor_empire must be provided when executing NCBI FCS Adaptor')
+    }
+
+    // Check for ncbi_fcs_gx_tax_id
+    if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) {
+        error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX')
+    }
+
+    // Check for ncbi_fcs_gx_db_path
+    if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) {
+        error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX')
+    }
+
+    // Check for busco_mode
+    if (!params.busco_skip && !params.busco_mode) {
+        error("busco_mode must be provided when executing BUSCO")
+    }
+
+    // Check for busco_lineage_datasets
+    if (!params.busco_skip && !params.busco_lineage_datasets) {
+        error('busco_lineage_datasets must be provided when executing BUSCO')
+    }
+
+    // Check for tidk_repeat_seq
+    if (!params.tidk_skip && !params.tidk_repeat_seq) {
+        error('tidk_repeat_seq must be provided when executing TIDK')
+    }
+
+    // Check for kraken2_db_path
+    if (!params.kraken2_skip && !params.kraken2_db_path) {
+        error('kraken2_db_path must be provided when executing Kraken2')
+    }
+}
+
+def validateInput(input) {
+    def inputFields = 5
+    def assemblyTags = input[(0..input.size()-1).step(inputFields)]
+
+    def tagCounts = [:]
+    assemblyTags.each { tag ->
+        tagCounts[tag] = tagCounts.containsKey(tag) ? tagCounts[tag] + 1 : 1
+    }
+    def repeatedTags = tagCounts.findAll { key, count -> count > 1 }.collect { key, count -> key }
+
+    if (repeatedTags.size() > 0) {
+        error("Please check input assemblysheet -> Multiple assemblies have the same tags!: ${repeatedTags}")
+    }
+
+    return input
+}
+
+def validateXrefAssemblies(xref) {
+    def xrefFields = 3
+    def xrefTags = xref[(0..xref.size()-1).step(xrefFields)]
+
+    def tagCounts = [:]
+    xrefTags.each { tag ->
+        tagCounts[tag] = tagCounts.containsKey(tag) ? tagCounts[tag] + 1 : 1
+    }
+    def repeatedTags = tagCounts.findAll { key, count -> count > 1 }.collect { key, count -> key }
+
+    if (repeatedTags.size() > 0) {
+        error("Please check synteny_xref_assemblies -> Multiple xref assemblies have the same tags!: ${repeatedTags}")
+    }
+
+    return xref
+}
+
+def jsonifyParams(params) {
+    return JsonOutput.toJson(params).toString()
+}
+
+def jsonifySummaryParams(params) {
+
+    def summary = [:]
+    for (group in params.keySet()) {
+        for (subgroup in params[group].keySet()) {
+            if ( params[group][subgroup] ) { summary << [ "$subgroup": "${params[group][subgroup]}" ] }
+        }
+    }
+
+    return JsonOutput.toJson(summary).toString()
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
new file mode 100644
index 00000000..ac31f28f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -0,0 +1,126 @@
+//
+// Subworkflow with functionality that may be useful for any Nextflow pipeline
+//
+
+import org.yaml.snakeyaml.Yaml
+import groovy.json.JsonOutput
+import nextflow.extension.FilesEx
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NEXTFLOW_PIPELINE {
+
+    take:
+    print_version        // boolean: print version
+    dump_parameters      // boolean: dump parameters
+    outdir               //    path: base directory used to publish pipeline results
+    check_conda_channels // boolean: check conda channels
+
+    main:
+
+    //
+    // Print workflow version and exit on --version
+    //
+    if (print_version) {
+        log.info "${workflow.manifest.name} ${getWorkflowVersion()}"
+        System.exit(0)
+    }
+
+    //
+    // Dump pipeline parameters to a JSON file
+    //
+    if (dump_parameters && outdir) {
+        dumpParametersToJSON(outdir)
+    }
+
+    //
+    // When running with Conda, warn if channels have not been set-up appropriately
+    //
+    if (check_conda_channels) {
+        checkCondaChannels()
+    }
+
+    emit:
+    dummy_emit = true
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+
+//
+// Generate version string
+//
+def getWorkflowVersion() {
+    String version_string = ""
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Dump pipeline parameters to a JSON file
+//
+def dumpParametersToJSON(outdir) {
+    def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
+    def filename   = "params_${timestamp}.json"
+    def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
+    def jsonStr    = JsonOutput.toJson(params)
+    temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
+
+    FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
+    temp_pf.delete()
+}
+
+//
+// When running with -profile conda, warn if channels have not been set-up appropriately
+//
+def checkCondaChannels() {
+    Yaml parser = new Yaml()
+    def channels = []
+    try {
+        def config = parser.load("conda config --show channels".execute().text)
+        channels = config.channels
+    } catch(NullPointerException | IOException e) {
+        log.warn "Could not verify conda channel configuration."
+        return
+    }
+
+    // Check that all channels are present
+    // This channel list is ordered by required channel priority.
+    def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
+    def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
+
+    // Check that they are in the right order
+    def channel_priority_violation = false
+    def n = required_channels_in_order.size()
+    for (int i = 0; i < n - 1; i++) {
+        channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
+    }
+
+    if (channels_missing | channel_priority_violation) {
+        log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            "  There is a problem with your Conda configuration!\n\n" +
+            "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
+            "  Please refer to https://bioconda.github.io/\n" +
+            "  The observed channel order is \n" +
+            "  ${channels}\n" +
+            "  but the following channel order is required:\n" +
+            "  ${required_channels_in_order}\n" +
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
new file mode 100644
index 00000000..e5c3a0a8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
@@ -0,0 +1,38 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NEXTFLOW_PIPELINE"
+description: Subworkflow with functionality that may be useful for any Nextflow pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - print_version:
+      type: boolean
+      description: |
+        Print the version of the pipeline and exit
+  - dump_parameters:
+      type: boolean
+      description: |
+        Dump the parameters of the pipeline to a JSON file
+  - output_directory:
+      type: directory
+      description: Path to output dir to write JSON file to.
+      pattern: "results/"
+  - check_conda_channel:
+      type: boolean
+      description: |
+        Check if the conda channel priority is correct.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+  - "@drpatelh"
+maintainers:
+  - "@adamrtalbot"
+  - "@drpatelh"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..68718e4f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
@@ -0,0 +1,54 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Test Function getWorkflowVersion") {
+
+        function "getWorkflowVersion"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dumpParametersToJSON") {
+
+        function "dumpParametersToJSON"
+
+        when {
+            function {
+                """
+                // define inputs of the function here. Example:
+                input[0] = "$outputDir"
+                """.stripIndent()
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success }
+            )
+        }
+    }
+
+    test("Test Function checkCondaChannels") {
+
+        function "checkCondaChannels"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..e3f0baf4
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,20 @@
+{
+    "Test Function getWorkflowVersion": {
+        "content": [
+            "v9.9.9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:05.308243"
+    },
+    "Test Function checkCondaChannels": {
+        "content": null,
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:12.425833"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..ca964ce8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,111 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NEXTFLOW_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    workflow "UTILS_NEXTFLOW_PIPELINE"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Should run no inputs") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should print version") {
+
+        when {
+            workflow {
+                """
+                print_version        = true
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.stdout.contains("nextflow_workflow v9.9.9") }
+            )
+        }
+    }
+
+    test("Should dump params") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = 'results'
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should not create params JSON if no output directory") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..d0a926bf
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
new file mode 100644
index 00000000..f8476112
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nextflow_pipeline:
+  - subworkflows/nf-core/utils_nextflow_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
new file mode 100644
index 00000000..a8b55d6f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -0,0 +1,440 @@
+//
+// Subworkflow with utility functions specific to the nf-core pipeline template
+//
+
+import org.yaml.snakeyaml.Yaml
+import nextflow.extension.FilesEx
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NFCORE_PIPELINE {
+
+    take:
+    nextflow_cli_args
+
+    main:
+    valid_config = checkConfigProvided()
+    checkProfileProvided(nextflow_cli_args)
+
+    emit:
+    valid_config
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+
+//
+//  Warn if a -profile or Nextflow config has not been provided to run the pipeline
+//
+def checkConfigProvided() {
+    valid_config = true
+    if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
+        log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
+            "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
+            "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
+            "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
+            "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
+            "Please refer to the quick start section and usage docs for the pipeline.\n "
+        valid_config = false
+    }
+    return valid_config
+}
+
+//
+// Exit pipeline if --profile contains spaces
+//
+def checkProfileProvided(nextflow_cli_args) {
+    if (workflow.profile.endsWith(',')) {
+        error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" +
+            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+    }
+    if (nextflow_cli_args[0]) {
+        log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" +
+            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+    }
+}
+
+//
+// Citation string for pipeline
+//
+def workflowCitation() {
+    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
+        "* The pipeline\n" +
+        "  ${workflow.manifest.doi}\n\n" +
+        "* The nf-core framework\n" +
+        "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
+        "* Software dependencies\n" +
+        "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
+}
+
+//
+// Generate workflow version string
+//
+def getWorkflowVersion() {
+    String version_string = ""
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Get software versions for pipeline
+//
+def processVersionsFromYAML(yaml_file) {
+    Yaml yaml = new Yaml()
+    versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] }
+    return yaml.dumpAsMap(versions).trim()
+}
+
+//
+// Get workflow version for pipeline
+//
+def workflowVersionToYAML() {
+    return """
+    Workflow:
+        $workflow.manifest.name: ${getWorkflowVersion()}
+        Nextflow: $workflow.nextflow.version
+    """.stripIndent().trim()
+}
+
+//
+// Get channel of software versions used in pipeline in YAML format
+//
+def softwareVersionsToYAML(ch_versions) {
+    return ch_versions
+                .unique()
+                .map { processVersionsFromYAML(it) }
+                .unique()
+                .mix(Channel.of(workflowVersionToYAML()))
+}
+
+//
+// Get workflow summary for MultiQC
+//
+def paramsSummaryMultiqc(summary_params) {
+    def summary_section = ''
+    for (group in summary_params.keySet()) {
+        def group_params = summary_params.get(group)  // This gets the parameters of that particular group
+        if (group_params) {
+            summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
+            summary_section += "    <dl class=\"dl-horizontal\">\n"
+            for (param in group_params.keySet()) {
+                summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+            }
+            summary_section += "    </dl>\n"
+        }
+    }
+
+    String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
+    yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
+    yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+    yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+    yaml_file_text        += "plot_type: 'html'\n"
+    yaml_file_text        += "data: |\n"
+    yaml_file_text        += "${summary_section}"
+
+    return yaml_file_text
+}
+
+//
+// nf-core logo
+//
+def nfCoreLogo(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    String.format(
+        """\n
+        ${dashedLine(monochrome_logs)}
+                                                ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
+        ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
+        ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
+        ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
+                                                ${colors.green}`._,._,\'${colors.reset}
+        ${colors.purple}  ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset}
+        ${dashedLine(monochrome_logs)}
+        """.stripIndent()
+    )
+}
+
+//
+// Return dashed line
+//
+def dashedLine(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+}
+
+//
+// ANSII colours used for terminal logging
+//
+def logColours(monochrome_logs=true) {
+    Map colorcodes = [:]
+
+    // Reset / Meta
+    colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
+    colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
+    colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
+    colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
+    colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
+    colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
+    colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
+
+    // Regular Colors
+    colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
+    colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
+    colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
+    colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
+    colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
+    colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
+    colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
+    colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
+
+    // Bold
+    colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
+    colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
+    colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
+    colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
+    colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
+    colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
+    colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
+    colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
+
+    // Underline
+    colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
+    colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
+    colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
+    colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
+    colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
+    colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
+    colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
+    colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
+
+    // High Intensity
+    colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
+    colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
+    colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
+    colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
+    colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
+    colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
+    colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
+    colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
+
+    // Bold High Intensity
+    colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
+    colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
+    colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
+    colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
+    colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
+    colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
+    colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
+    colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
+
+    return colorcodes
+}
+
+//
+// Attach the multiqc report to email
+//
+def attachMultiqcReport(multiqc_report) {
+    def mqc_report = null
+    try {
+        if (workflow.success) {
+            mqc_report = multiqc_report.getVal()
+            if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
+                if (mqc_report.size() > 1) {
+                    log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
+                }
+                mqc_report = mqc_report[0]
+            }
+        }
+    } catch (all) {
+        if (multiqc_report) {
+            log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
+        }
+    }
+    return mqc_report
+}
+
+//
+// Construct and send completion email
+//
+def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) {
+
+    // Set up the e-mail variables
+    def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
+    if (!workflow.success) {
+        subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
+    }
+
+    def summary = [:]
+    for (group in summary_params.keySet()) {
+        summary << summary_params[group]
+    }
+
+    def misc_fields = [:]
+    misc_fields['Date Started']              = workflow.start
+    misc_fields['Date Completed']            = workflow.complete
+    misc_fields['Pipeline script file path'] = workflow.scriptFile
+    misc_fields['Pipeline script hash ID']   = workflow.scriptId
+    if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
+    if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+    if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
+    misc_fields['Nextflow Version']           = workflow.nextflow.version
+    misc_fields['Nextflow Build']             = workflow.nextflow.build
+    misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
+
+    def email_fields = [:]
+    email_fields['version']      = getWorkflowVersion()
+    email_fields['runName']      = workflow.runName
+    email_fields['success']      = workflow.success
+    email_fields['dateComplete'] = workflow.complete
+    email_fields['duration']     = workflow.duration
+    email_fields['exitStatus']   = workflow.exitStatus
+    email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    email_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    email_fields['commandLine']  = workflow.commandLine
+    email_fields['projectDir']   = workflow.projectDir
+    email_fields['summary']      = summary << misc_fields
+
+    // On success try attach the multiqc report
+    def mqc_report = attachMultiqcReport(multiqc_report)
+
+    // Check if we are only sending emails on failure
+    def email_address = email
+    if (!email && email_on_fail && !workflow.success) {
+        email_address = email_on_fail
+    }
+
+    // Render the TXT template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    def tf           = new File("${workflow.projectDir}/assets/email_template.txt")
+    def txt_template = engine.createTemplate(tf).make(email_fields)
+    def email_txt    = txt_template.toString()
+
+    // Render the HTML template
+    def hf            = new File("${workflow.projectDir}/assets/email_template.html")
+    def html_template = engine.createTemplate(hf).make(email_fields)
+    def email_html    = html_template.toString()
+
+    // Render the sendmail template
+    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
+    def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
+    def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
+    def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
+    def sendmail_html          = sendmail_template.toString()
+
+    // Send the HTML e-mail
+    Map colors = logColours(monochrome_logs)
+    if (email_address) {
+        try {
+            if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
+            // Try to send HTML e-mail using sendmail
+            def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
+            sendmail_tf.withWriter { w -> w << sendmail_html }
+            [ 'sendmail', '-t' ].execute() << sendmail_html
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
+        } catch (all) {
+            // Catch failures and try with plaintext
+            def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+            mail_cmd.execute() << email_html
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
+        }
+    }
+
+    // Write summary e-mail HTML to a file
+    def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
+    output_hf.withWriter { w -> w << email_html }
+    FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html");
+    output_hf.delete()
+
+    // Write summary e-mail TXT to a file
+    def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
+    output_tf.withWriter { w -> w << email_txt }
+    FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt");
+    output_tf.delete()
+}
+
+//
+// Print pipeline summary on completion
+//
+def completionSummary(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    if (workflow.success) {
+        if (workflow.stats.ignoredCount == 0) {
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
+        } else {
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+        }
+    } else {
+        log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
+    }
+}
+
+//
+// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack
+//
+def imNotification(summary_params, hook_url) {
+    def summary = [:]
+    for (group in summary_params.keySet()) {
+        summary << summary_params[group]
+    }
+
+    def misc_fields = [:]
+    misc_fields['start']                                = workflow.start
+    misc_fields['complete']                             = workflow.complete
+    misc_fields['scriptfile']                           = workflow.scriptFile
+    misc_fields['scriptid']                             = workflow.scriptId
+    if (workflow.repository) misc_fields['repository']  = workflow.repository
+    if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
+    if (workflow.revision)   misc_fields['revision']    = workflow.revision
+    misc_fields['nxf_version']                          = workflow.nextflow.version
+    misc_fields['nxf_build']                            = workflow.nextflow.build
+    misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
+
+    def msg_fields = [:]
+    msg_fields['version']      = getWorkflowVersion()
+    msg_fields['runName']      = workflow.runName
+    msg_fields['success']      = workflow.success
+    msg_fields['dateComplete'] = workflow.complete
+    msg_fields['duration']     = workflow.duration
+    msg_fields['exitStatus']   = workflow.exitStatus
+    msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
+    msg_fields['projectDir']   = workflow.projectDir
+    msg_fields['summary']      = summary << misc_fields
+
+    // Render the JSON template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    // Different JSON depending on the service provider
+    // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
+    def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
+    def hf            = new File("${workflow.projectDir}/assets/${json_path}")
+    def json_template = engine.createTemplate(hf).make(msg_fields)
+    def json_message  = json_template.toString()
+
+    // POST
+    def post = new URL(hook_url).openConnection();
+    post.setRequestMethod("POST")
+    post.setDoOutput(true)
+    post.setRequestProperty("Content-Type", "application/json")
+    post.getOutputStream().write(json_message.getBytes("UTF-8"));
+    def postRC = post.getResponseCode();
+    if (! postRC.equals(200)) {
+        log.warn(post.getErrorStream().getText());
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
new file mode 100644
index 00000000..d08d2434
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
@@ -0,0 +1,24 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFCORE_PIPELINE"
+description: Subworkflow with utility functions specific to the nf-core pipeline template
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - nextflow_cli_args:
+      type: list
+      description: |
+        Nextflow CLI positional arguments
+output:
+  - success:
+      type: boolean
+      description: |
+        Dummy output to indicate success
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..1dc317f8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
@@ -0,0 +1,134 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Test Function checkConfigProvided") {
+
+        function "checkConfigProvided"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function checkProfileProvided") {
+
+        function "checkProfileProvided"
+
+        when {
+            function {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function workflowCitation") {
+
+        function "workflowCitation"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function nfCoreLogo") {
+
+        function "nfCoreLogo"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dashedLine") {
+
+        function "dashedLine"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function without logColours") {
+
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function with logColours") {
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..1037232c
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,166 @@
+{
+    "Test Function checkProfileProvided": {
+        "content": null,
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:03.360873"
+    },
+    "Test Function checkConfigProvided": {
+        "content": [
+            true
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:59.729647"
+    },
+    "Test Function nfCoreLogo": {
+        "content": [
+            "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n                                        \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m        ___     __   __   __   ___     \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \u001b[0;33m}  {\u001b[0m\n\u001b[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \u001b[0;32m\\`-._,-`-,\u001b[0m\n                                        \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m  nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:10.562934"
+    },
+    "Test Function workflowCitation": {
+        "content": [
+            "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n  https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n  https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n  https://github.com/nextflow_workflow/blob/master/CITATIONS.md"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:07.019761"
+    },
+    "Test Function without logColours": {
+        "content": [
+            {
+                "reset": "",
+                "bold": "",
+                "dim": "",
+                "underlined": "",
+                "blink": "",
+                "reverse": "",
+                "hidden": "",
+                "black": "",
+                "red": "",
+                "green": "",
+                "yellow": "",
+                "blue": "",
+                "purple": "",
+                "cyan": "",
+                "white": "",
+                "bblack": "",
+                "bred": "",
+                "bgreen": "",
+                "byellow": "",
+                "bblue": "",
+                "bpurple": "",
+                "bcyan": "",
+                "bwhite": "",
+                "ublack": "",
+                "ured": "",
+                "ugreen": "",
+                "uyellow": "",
+                "ublue": "",
+                "upurple": "",
+                "ucyan": "",
+                "uwhite": "",
+                "iblack": "",
+                "ired": "",
+                "igreen": "",
+                "iyellow": "",
+                "iblue": "",
+                "ipurple": "",
+                "icyan": "",
+                "iwhite": "",
+                "biblack": "",
+                "bired": "",
+                "bigreen": "",
+                "biyellow": "",
+                "biblue": "",
+                "bipurple": "",
+                "bicyan": "",
+                "biwhite": ""
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:17.969323"
+    },
+    "Test Function dashedLine": {
+        "content": [
+            "-\u001b[2m----------------------------------------------------\u001b[0m-"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:14.366181"
+    },
+    "Test Function with logColours": {
+        "content": [
+            {
+                "reset": "\u001b[0m",
+                "bold": "\u001b[1m",
+                "dim": "\u001b[2m",
+                "underlined": "\u001b[4m",
+                "blink": "\u001b[5m",
+                "reverse": "\u001b[7m",
+                "hidden": "\u001b[8m",
+                "black": "\u001b[0;30m",
+                "red": "\u001b[0;31m",
+                "green": "\u001b[0;32m",
+                "yellow": "\u001b[0;33m",
+                "blue": "\u001b[0;34m",
+                "purple": "\u001b[0;35m",
+                "cyan": "\u001b[0;36m",
+                "white": "\u001b[0;37m",
+                "bblack": "\u001b[1;30m",
+                "bred": "\u001b[1;31m",
+                "bgreen": "\u001b[1;32m",
+                "byellow": "\u001b[1;33m",
+                "bblue": "\u001b[1;34m",
+                "bpurple": "\u001b[1;35m",
+                "bcyan": "\u001b[1;36m",
+                "bwhite": "\u001b[1;37m",
+                "ublack": "\u001b[4;30m",
+                "ured": "\u001b[4;31m",
+                "ugreen": "\u001b[4;32m",
+                "uyellow": "\u001b[4;33m",
+                "ublue": "\u001b[4;34m",
+                "upurple": "\u001b[4;35m",
+                "ucyan": "\u001b[4;36m",
+                "uwhite": "\u001b[4;37m",
+                "iblack": "\u001b[0;90m",
+                "ired": "\u001b[0;91m",
+                "igreen": "\u001b[0;92m",
+                "iyellow": "\u001b[0;93m",
+                "iblue": "\u001b[0;94m",
+                "ipurple": "\u001b[0;95m",
+                "icyan": "\u001b[0;96m",
+                "iwhite": "\u001b[0;97m",
+                "biblack": "\u001b[1;90m",
+                "bired": "\u001b[1;91m",
+                "bigreen": "\u001b[1;92m",
+                "biyellow": "\u001b[1;93m",
+                "biblue": "\u001b[1;94m",
+                "bipurple": "\u001b[1;95m",
+                "bicyan": "\u001b[1;96m",
+                "biwhite": "\u001b[1;97m"
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:21.714424"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..8940d32d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,29 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFCORE_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    workflow "UTILS_NFCORE_PIPELINE"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Should run without failures") {
+
+        when {
+            workflow {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
new file mode 100644
index 00000000..859d1030
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
@@ -0,0 +1,19 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    true
+                ],
+                "valid_config": [
+                    true
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:25.726491"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..d0a926bf
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
new file mode 100644
index 00000000..ac8523c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfcore_pipeline:
+  - subworkflows/nf-core/utils_nfcore_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
new file mode 100644
index 00000000..2585b65d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
@@ -0,0 +1,62 @@
+//
+// Subworkflow that uses the nf-validation plugin to render help text and parameter summary
+//
+
+/*
+========================================================================================
+    IMPORT NF-VALIDATION PLUGIN
+========================================================================================
+*/
+
+include { paramsHelp         } from 'plugin/nf-validation'
+include { paramsSummaryLog   } from 'plugin/nf-validation'
+include { validateParameters } from 'plugin/nf-validation'
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NFVALIDATION_PLUGIN {
+
+    take:
+    print_help       // boolean: print help
+    workflow_command //  string: default commmand used to run pipeline
+    pre_help_text    //  string: string to be printed before help text and summary log
+    post_help_text   //  string: string to be printed after help text and summary log
+    validate_params  // boolean: validate parameters
+    schema_filename  //    path: JSON schema file, null to use default value
+
+    main:
+
+    log.debug "Using schema file: ${schema_filename}"
+
+    // Default values for strings
+    pre_help_text    = pre_help_text    ?: ''
+    post_help_text   = post_help_text   ?: ''
+    workflow_command = workflow_command ?: ''
+
+    //
+    // Print help message if needed
+    //
+    if (print_help) {
+        log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text
+        System.exit(0)
+    }
+
+    //
+    // Print parameter summary to stdout
+    //
+    log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text
+
+    //
+    // Validate parameters relative to the parameter JSON schema
+    //
+    if (validate_params){
+        validateParameters(parameters_schema: schema_filename)
+    }
+
+    emit:
+    dummy_emit = true
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
new file mode 100644
index 00000000..3d4a6b04
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
@@ -0,0 +1,44 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFVALIDATION_PLUGIN"
+description: Use nf-validation to initiate and validate a pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - validation
+components: []
+input:
+  - print_help:
+      type: boolean
+      description: |
+        Print help message and exit
+  - workflow_command:
+      type: string
+      description: |
+        The command to run the workflow e.g. "nextflow run main.nf"
+  - pre_help_text:
+      type: string
+      description: |
+        Text to print before the help message
+  - post_help_text:
+      type: string
+      description: |
+        Text to print after the help message
+  - validate_params:
+      type: boolean
+      description: |
+        Validate the parameters and error if invalid.
+  - schema_filename:
+      type: string
+      description: |
+        The filename of the schema to validate against.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
new file mode 100644
index 00000000..5784a33f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
@@ -0,0 +1,200 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFVALIDATION_PLUGIN"
+    script "../main.nf"
+    workflow "UTILS_NFVALIDATION_PLUGIN"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "plugin/nf-validation"
+    tag "'plugin/nf-validation'"
+    tag "utils_nfvalidation_plugin"
+    tag "subworkflows/utils_nfvalidation_plugin"
+
+    test("Should run nothing") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+
+            workflow {
+                """
+                help             = false
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should run help") {
+
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } }
+            )
+        }
+    }
+
+    test("Should run help with command") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = "nextflow run noorg/doesntexist"
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } }
+            )
+        }
+    }
+
+    test("Should run help with extra text") {
+
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = "nextflow run noorg/doesntexist"
+                pre_help_text    = "pre-help-text"
+                post_help_text   = "post-help-text"
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('pre-help-text') } },
+                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } },
+                { assert workflow.stdout.any { it.contains('post-help-text') } }
+            )
+        }
+    }
+
+    test("Should validate params") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+                outdir          = 1
+            }
+            workflow {
+                """
+                help             = false
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = true
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
new file mode 100644
index 00000000..7626c1c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
@@ -0,0 +1,96 @@
+{
+    "$schema": "http://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json",
+    "title": ". pipeline parameters",
+    "description": "",
+    "type": "object",
+    "definitions": {
+        "input_output_options": {
+            "title": "Input/output options",
+            "type": "object",
+            "fa_icon": "fas fa-terminal",
+            "description": "Define where the pipeline should find input data and save output data.",
+            "required": ["outdir"],
+            "properties": {
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Validate parameters?",
+                    "default": true,
+                    "hidden": true
+                },
+                "outdir": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "test_data_base": {
+                    "type": "string",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules",
+                    "description": "Base for test data directory",
+                    "hidden": true
+                },
+                "test_data": {
+                    "type": "string",
+                    "description": "Fake test data param",
+                    "hidden": true
+                }
+            }
+        },
+        "generic_options": {
+            "title": "Generic options",
+            "type": "object",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "version": {
+                    "type": "boolean",
+                    "description": "Display version and exit.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "logo": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Display nf-core logo in console output.",
+                    "fa_icon": "fas fa-image",
+                    "hidden": true
+                },
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Pull Singularity container from Docker?",
+                    "hidden": true
+                },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "hidden": true
+                },
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Use monochrome_logs",
+                    "hidden": true
+                }
+            }
+        }
+    },
+    "allOf": [
+        {
+            "$ref": "#/definitions/input_output_options"
+        },
+        {
+            "$ref": "#/definitions/generic_options"
+        }
+    ]
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
new file mode 100644
index 00000000..60b1cfff
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfvalidation_plugin:
+  - subworkflows/nf-core/utils_nfvalidation_plugin/**
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 38217ec5..ea228334 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -1,31 +1,22 @@
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    PRINT PARAMS SUMMARY
+    IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation'
+include { validateInput                     } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
+include { validateXrefAssemblies            } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
+include { jsonifyParams                     } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
+include { jsonifySummaryParams              } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
 
-def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-def summary_params = paramsSummaryMap(workflow)
+include { paramsSummaryMap                  } from 'plugin/nf-validation'
+include { paramsSummaryMultiqc              } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML            } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
-// Print parameter summary log to screen
-log.info logo + paramsSummaryLog(workflow) + citation
-
-WorkflowAssemblyqc.initialise(params, log)
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    CONFIG FILES
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
+include { GUNZIP as GUNZIP_FASTA            } from '../modules/nf-core/gunzip/main'
+include { GUNZIP as GUNZIP_GFF3             } from '../modules/nf-core/gunzip/main'
+include { FASTAVALIDATOR                    } from '../modules/nf-core/fastavalidator/main'
+include { FASTA_EXPLORE_SEARCH_PLOT_TIDK    } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main'
 
 include { GT_STAT                           } from '../modules/pfr/gt/stat/main'
 include { GFF3_VALIDATE                     } from '../subworkflows/pfr/gff3_validate/main'
@@ -39,24 +30,6 @@ include { FQ2HIC                            } from '../subworkflows/local/fq2hic
 include { FASTA_SYNTENY                     } from '../subworkflows/local/fasta_synteny'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// MODULE: Installed directly from nf-core/modules
-//
-
-include { GUNZIP as GUNZIP_FASTA            } from '../modules/nf-core/gunzip/main'
-include { GUNZIP as GUNZIP_GFF3             } from '../modules/nf-core/gunzip/main'
-include { FASTAVALIDATOR                    } from '../modules/nf-core/fastavalidator/main'
-include { FASTA_EXPLORE_SEARCH_PLOT_TIDK    } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main'
-
-include { CUSTOM_DUMPSOFTWAREVERSIONS       } from '../modules/nf-core/custom/dumpsoftwareversions/main'
-
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -72,7 +45,7 @@ workflow ASSEMBLYQC {
     ch_versions                             = Channel.empty()
     ch_input                                = Channel.fromSamplesheet('input')
                                             | collect
-                                            | flatMap { WorkflowAssemblyqc.validateInput(it) }
+                                            | flatMap { validateInput(it) }
                                             | buffer(size: input_assembly_sheet_fields)
 
     ch_target_assemby_branch                = ch_input
@@ -130,7 +103,7 @@ workflow ASSEMBLYQC {
                                             ? Channel.empty()
                                             : Channel.fromSamplesheet('synteny_xref_assemblies')
                                             | collect
-                                            | flatMap { WorkflowAssemblyqc.validateXrefAssemblies(it) }
+                                            | flatMap { validateXrefAssemblies(it) }
                                             | buffer(size: synteny_xref_assemblies_fields)
                                             | map { tag, fa, labels ->
                                                 [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ]
@@ -424,9 +397,13 @@ workflow ASSEMBLYQC {
     ch_synteny_plot                         = FASTA_SYNTENY.out.plot
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
-    // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS
-    CUSTOM_DUMPSOFTWAREVERSIONS (
-        ch_versions.unique().collectFile(name: 'collated_versions.yml')
+    // Collate and save software versions
+    softwareVersionsToYAML ( ch_versions )
+    | collectFile(
+        storeDir: "${params.outdir}/pipeline_info",
+        name: 'nf_core_pipeline_software_mqc_versions.yml',
+        sort: true,
+        newLine: true
     )
 
     // MODULE: CREATEREPORT
@@ -444,28 +421,11 @@ workflow ASSEMBLYQC {
         ch_hic_html                         .collect().ifEmpty([]),
         ch_synteny_plot                     .collect().ifEmpty([]),
         CUSTOM_DUMPSOFTWAREVERSIONS         .out.yml,
-        Channel.of ( WorkflowAssemblyqc.jsonifyParams ( params ) ),
-        Channel.of ( WorkflowAssemblyqc.jsonifySummaryParams ( summary_params ) )
+        Channel.of ( jsonifyParams ( params ) ),
+        Channel.of ( jsonifySummaryParams ( summary_params ) )
     )
 }
 
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    COMPLETION EMAIL AND SUMMARY
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-workflow.onComplete {
-    if (params.email || params.email_on_fail) {
-        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log)
-    }
-    NfcoreTemplate.dump_parameters(workflow, params)
-    NfcoreTemplate.summary(workflow, params, log)
-    if (params.hook_url) {
-        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-    }
-}
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     THE END

From 082df60946c8b399012a10372ed393684521c778 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 14:47:40 +1200
Subject: [PATCH 05/76] Moved input channel init to sub workflow

---
 main.nf                                       | 24 ++++-
 nextflow.config                               |  1 +
 .../utils_nfcore_assemblyqc_pipeline/main.nf  | 47 ++++++++-
 workflows/assemblyqc.nf                       | 97 +++++++------------
 4 files changed, 104 insertions(+), 65 deletions(-)

diff --git a/main.nf b/main.nf
index 7a002146..eb7e9e94 100755
--- a/main.nf
+++ b/main.nf
@@ -30,10 +30,24 @@ include { PIPELINE_COMPLETION       } from './subworkflows/local/utils_nfcore_as
 //
 workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
 
+    take:
+    ch_input
+    ch_hic_reads
+    ch_xref_assembly
+    ch_params_as_json
+    ch_summary_params_as_json
+
+    main:
     //
     // WORKFLOW: Run pipeline
     //
-    ASSEMBLYQC ()
+    ASSEMBLYQC (
+        ch_input,
+        ch_hic_reads,
+        ch_xref_assembly,
+        ch_params_as_json,
+        ch_summary_params_as_json
+    )
 
 }
 /*
@@ -62,7 +76,13 @@ workflow {
     //
     // WORKFLOW: Run main workflow
     //
-    PLANTFOODRESEARCHOPEN_ASSEMBLYQC ()
+    PLANTFOODRESEARCHOPEN_ASSEMBLYQC (
+        PIPELINE_INITIALISATION.out.input,
+        PIPELINE_INITIALISATION.out.hic_reads,
+        PIPELINE_INITIALISATION.out.xref_assembly,
+        PIPELINE_INITIALISATION.out.params_as_json,
+        PIPELINE_INITIALISATION.out.summary_params_as_json
+    )
 
     //
     // SUBWORKFLOW: Run completion tasks
diff --git a/nextflow.config b/nextflow.config
index c110c029..84ddfb0c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -72,6 +72,7 @@ params {
     email_on_fail                       = null
     plaintext_email                     = false
     monochrome_logs                     = false
+    monochromeLogs                      = false
     hook_url                            = null
     help                                = false
     version                             = false
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 7013d07c..e3433f54 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -41,7 +41,8 @@ workflow PIPELINE_INITIALISATION {
 
     main:
 
-    ch_versions = Channel.empty()
+    ch_versions     = Channel.empty()
+    summary_params  = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
 
     //
     // Print version and exit if required and dump pipeline parameters to JSON file
@@ -74,13 +75,55 @@ workflow PIPELINE_INITIALISATION {
     UTILS_NFCORE_PIPELINE (
         nextflow_cli_args
     )
+
     //
     // Custom validation for pipeline parameters
     //
     validateInputParameters()
 
+    //
+    // Initialise input channels
+    //
+    def input_assembly_sheet_fields         = 5
+    def synteny_xref_assemblies_fields      = 3
+
+    ch_input                                = Channel.fromSamplesheet('input')
+                                            | collect
+                                            | flatMap { validateInput(it) }
+                                            | buffer(size: input_assembly_sheet_fields)
+
+    ch_hic_reads                            = ! params.hic
+                                            ? Channel.empty()
+                                            : (
+                                                "$params.hic".find(/.*[\/].*\.(fastq|fq)\.gz/)
+                                                ? Channel.fromFilePairs(params.hic, checkIfExists: true)
+                                                : Channel.fromSRA(params.hic)
+                                            )
+                                            | map{ sample, fq ->
+                                                [ [ id: sample, single_end: false ], fq ]
+                                            }
+
+    ch_xref_assembly                        = params.synteny_skip || ! params.synteny_xref_assemblies
+                                            ? Channel.empty()
+                                            : Channel.fromSamplesheet('synteny_xref_assemblies')
+                                            | collect
+                                            | flatMap { validateXrefAssemblies(it) }
+                                            | buffer(size: synteny_xref_assemblies_fields)
+                                            | map { tag, fa, labels ->
+                                                [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ]
+                                            }
+
+    // Initialise parameter channels
+    ch_params_as_json                       = Channel.of ( jsonifyParams ( params ) )
+    ch_summary_params_as_json               = Channel.of ( jsonifySummaryParams ( summary_params ) )
+
     emit:
-    versions    = ch_versions
+    input                                   = ch_input
+    hic_reads                               = ch_hic_reads
+    xref_assembly                           = ch_xref_assembly
+    params_as_json                          = ch_params_as_json
+    summary_params_as_json                  = ch_summary_params_as_json
+    versions                                = ch_versions
 }
 
 /*
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index ea228334..2b25993e 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -4,13 +4,6 @@
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { validateInput                     } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
-include { validateXrefAssemblies            } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
-include { jsonifyParams                     } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
-include { jsonifySummaryParams              } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline/main'
-
-include { paramsSummaryMap                  } from 'plugin/nf-validation'
-include { paramsSummaryMultiqc              } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML            } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
 include { GUNZIP as GUNZIP_FASTA            } from '../modules/nf-core/gunzip/main'
@@ -36,18 +29,21 @@ include { CREATEREPORT                      } from '../modules/local/createrepor
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-def input_assembly_sheet_fields             = 5
-def synteny_xref_assemblies_fields          = 3
-
 workflow ASSEMBLYQC {
 
-    // Input channels
+    take:
+    ch_input
+    ch_hic_reads
+    ch_xref_assembly
+    ch_params_as_json
+    ch_summary_params_as_json
+
+    main:
+
+    // Versions
     ch_versions                             = Channel.empty()
-    ch_input                                = Channel.fromSamplesheet('input')
-                                            | collect
-                                            | flatMap { validateInput(it) }
-                                            | buffer(size: input_assembly_sheet_fields)
 
+    // Input channels
     ch_target_assemby_branch                = ch_input
                                             | map { tag, fasta, gff, mono_ids, labels ->
                                                 [ [ id: tag ], file(fasta, checkIfExists: true) ]
@@ -88,27 +84,6 @@ workflow ASSEMBLYQC {
                                                 )
                                             }
 
-    ch_hic_reads                            = ! params.hic
-                                            ? Channel.empty()
-                                            : (
-                                                "$params.hic".find(/.*[\/].*\.(fastq|fq)\.gz/)
-                                                ? Channel.fromFilePairs(params.hic, checkIfExists: true)
-                                                : Channel.fromSRA(params.hic)
-                                            )
-                                            | map{ sample, fq ->
-                                                [ [ id: sample, single_end: false ], fq ]
-                                            }
-
-    ch_xref_assembly                        = params.synteny_skip || ! params.synteny_xref_assemblies
-                                            ? Channel.empty()
-                                            : Channel.fromSamplesheet('synteny_xref_assemblies')
-                                            | collect
-                                            | flatMap { validateXrefAssemblies(it) }
-                                            | buffer(size: synteny_xref_assemblies_fields)
-                                            | map { tag, fa, labels ->
-                                                [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ]
-                                            }
-
     // MODULE: GUNZIP as GUNZIP_FASTA
     GUNZIP_FASTA ( ch_target_assemby_branch.gz )
 
@@ -398,32 +373,32 @@ workflow ASSEMBLYQC {
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
     // Collate and save software versions
-    softwareVersionsToYAML ( ch_versions )
-    | collectFile(
-        storeDir: "${params.outdir}/pipeline_info",
-        name: 'nf_core_pipeline_software_mqc_versions.yml',
-        sort: true,
-        newLine: true
-    )
+    ch_versions_yml                         = softwareVersionsToYAML(ch_versions)
+                                            | collectFile(
+                                                storeDir: "${params.outdir}/pipeline_info",
+                                                name: 'nf_core_pipeline_software_mqc_versions.yml',
+                                                sort: true,
+                                                newLine: true
+                                            )
 
-    // MODULE: CREATEREPORT
-    CREATEREPORT(
-        ch_invalid_assembly_log             .map { meta, file -> file }.collect().ifEmpty([]),
-        ch_invalid_gff3_log                 .map { meta, file -> file }.collect().ifEmpty([]),
-        ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
-        ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
-        ch_assemblathon_stats               .collect().ifEmpty([]),
-        ch_gt_stats                         .collect().ifEmpty([]),
-        ch_busco_summary                    .mix(ch_busco_plot).collect().ifEmpty([]),
-        ch_tidk_outputs                     .collect().ifEmpty([]),
-        ch_lai_outputs                      .collect().ifEmpty([]),
-        ch_kraken2_plot                     .collect().ifEmpty([]),
-        ch_hic_html                         .collect().ifEmpty([]),
-        ch_synteny_plot                     .collect().ifEmpty([]),
-        CUSTOM_DUMPSOFTWAREVERSIONS         .out.yml,
-        Channel.of ( jsonifyParams ( params ) ),
-        Channel.of ( jsonifySummaryParams ( summary_params ) )
-    )
+    // // MODULE: CREATEREPORT
+    // CREATEREPORT(
+    //     ch_invalid_assembly_log             .map { meta, file -> file }.collect().ifEmpty([]),
+    //     ch_invalid_gff3_log                 .map { meta, file -> file }.collect().ifEmpty([]),
+    //     ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
+    //     ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
+    //     ch_assemblathon_stats               .collect().ifEmpty([]),
+    //     ch_gt_stats                         .collect().ifEmpty([]),
+    //     ch_busco_summary                    .mix(ch_busco_plot).collect().ifEmpty([]),
+    //     ch_tidk_outputs                     .collect().ifEmpty([]),
+    //     ch_lai_outputs                      .collect().ifEmpty([]),
+    //     ch_kraken2_plot                     .collect().ifEmpty([]),
+    //     ch_hic_html                         .collect().ifEmpty([]),
+    //     ch_synteny_plot                     .collect().ifEmpty([]),
+    //     ch_versions_yml,
+    //     ch_params_as_json,
+    //     ch_summary_params_as_json
+    // )
 }
 
 /*

From 15fdae0e2be7111a4728d9122b610ae0c63c8fd1 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:04:06 +1200
Subject: [PATCH 06/76] Fixed null versions.yml issue

---
 workflows/assemblyqc.nf | 44 +++++++++++++++++++++++------------------
 1 file changed, 25 insertions(+), 19 deletions(-)

diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 2b25993e..efb6cf15 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -373,32 +373,38 @@ workflow ASSEMBLYQC {
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
     // Collate and save software versions
+    ch_versions                             = ch_versions
+                                            | unique
+                                            | map { yml ->
+                                                if ( yml ) { yml }
+                                            }
+
     ch_versions_yml                         = softwareVersionsToYAML(ch_versions)
                                             | collectFile(
                                                 storeDir: "${params.outdir}/pipeline_info",
-                                                name: 'nf_core_pipeline_software_mqc_versions.yml',
+                                                name: 'software_versions.yml',
                                                 sort: true,
                                                 newLine: true
                                             )
 
-    // // MODULE: CREATEREPORT
-    // CREATEREPORT(
-    //     ch_invalid_assembly_log             .map { meta, file -> file }.collect().ifEmpty([]),
-    //     ch_invalid_gff3_log                 .map { meta, file -> file }.collect().ifEmpty([]),
-    //     ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
-    //     ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
-    //     ch_assemblathon_stats               .collect().ifEmpty([]),
-    //     ch_gt_stats                         .collect().ifEmpty([]),
-    //     ch_busco_summary                    .mix(ch_busco_plot).collect().ifEmpty([]),
-    //     ch_tidk_outputs                     .collect().ifEmpty([]),
-    //     ch_lai_outputs                      .collect().ifEmpty([]),
-    //     ch_kraken2_plot                     .collect().ifEmpty([]),
-    //     ch_hic_html                         .collect().ifEmpty([]),
-    //     ch_synteny_plot                     .collect().ifEmpty([]),
-    //     ch_versions_yml,
-    //     ch_params_as_json,
-    //     ch_summary_params_as_json
-    // )
+    // MODULE: CREATEREPORT
+    CREATEREPORT(
+        ch_invalid_assembly_log             .map { meta, file -> file }.collect().ifEmpty([]),
+        ch_invalid_gff3_log                 .map { meta, file -> file }.collect().ifEmpty([]),
+        ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
+        ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
+        ch_assemblathon_stats               .collect().ifEmpty([]),
+        ch_gt_stats                         .collect().ifEmpty([]),
+        ch_busco_summary                    .mix(ch_busco_plot).collect().ifEmpty([]),
+        ch_tidk_outputs                     .collect().ifEmpty([]),
+        ch_lai_outputs                      .collect().ifEmpty([]),
+        ch_kraken2_plot                     .collect().ifEmpty([]),
+        ch_hic_html                         .collect().ifEmpty([]),
+        ch_synteny_plot                     .collect().ifEmpty([]),
+        ch_versions_yml,
+        ch_params_as_json,
+        ch_summary_params_as_json
+    )
 }
 
 /*

From 177c007156a069df5c058f23b8c5bc15bfb2e876 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:10:48 +1200
Subject: [PATCH 07/76] Updated modules json

---
 modules.json                                  | 12 +--
 modules/nf-core/multiqc/main.nf               | 55 ------------
 modules/nf-core/multiqc/meta.yml              | 58 -------------
 modules/nf-core/multiqc/tests/main.nf.test    | 84 -------------------
 .../nf-core/multiqc/tests/main.nf.test.snap   | 41 ---------
 5 files changed, 4 insertions(+), 246 deletions(-)
 delete mode 100644 modules/nf-core/multiqc/main.nf
 delete mode 100644 modules/nf-core/multiqc/meta.yml
 delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test
 delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap

diff --git a/modules.json b/modules.json
index 564e7abd..2428508b 100644
--- a/modules.json
+++ b/modules.json
@@ -134,13 +134,6 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
-                    "custom/dumpsoftwareversions": {
-                        "branch": "master",
-                        "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1",
-                        "installed_by": [
-                            "modules"
-                        ]
-                    },
                     "fastavalidator": {
                         "branch": "master",
                         "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
@@ -225,6 +218,9 @@
                     "fastq_trim_fastp_fastqc": {
                         "branch": "master",
                         "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
@@ -251,4 +247,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
deleted file mode 100644
index 47ac352f..00000000
--- a/modules/nf-core/multiqc/main.nf
+++ /dev/null
@@ -1,55 +0,0 @@
-process MULTIQC {
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.21--pyhdfd78af_0' }"
-
-    input:
-    path  multiqc_files, stageAs: "?/*"
-    path(multiqc_config)
-    path(extra_multiqc_config)
-    path(multiqc_logo)
-
-    output:
-    path "*multiqc_report.html", emit: report
-    path "*_data"              , emit: data
-    path "*_plots"             , optional:true, emit: plots
-    path "versions.yml"        , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def config = multiqc_config ? "--config $multiqc_config" : ''
-    def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
-    def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : ''
-    """
-    multiqc \\
-        --force \\
-        $args \\
-        $config \\
-        $extra_config \\
-        $logo \\
-        .
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    mkdir multiqc_data
-    touch multiqc_plots
-    touch multiqc_report.html
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
deleted file mode 100644
index 45a9bc35..00000000
--- a/modules/nf-core/multiqc/meta.yml
+++ /dev/null
@@ -1,58 +0,0 @@
-name: multiqc
-description: Aggregate results from bioinformatics analyses across many samples into a single report
-keywords:
-  - QC
-  - bioinformatics tools
-  - Beautiful stand-alone HTML report
-tools:
-  - multiqc:
-      description: |
-        MultiQC searches a given directory for analysis logs and compiles a HTML report.
-        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
-      homepage: https://multiqc.info/
-      documentation: https://multiqc.info/docs/
-      licence: ["GPL-3.0-or-later"]
-input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
-      pattern: "*.{yml,yaml}"
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
-output:
-  - report:
-      type: file
-      description: MultiQC report file
-      pattern: "multiqc_report.html"
-  - data:
-      type: directory
-      description: MultiQC data dir
-      pattern: "multiqc_data"
-  - plots:
-      type: file
-      description: Plots created by MultiQC
-      pattern: "*_data"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@bunop"
-  - "@drpatelh"
-  - "@jfy133"
-maintainers:
-  - "@abhi18av"
-  - "@bunop"
-  - "@drpatelh"
-  - "@jfy133"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
deleted file mode 100644
index f1c4242e..00000000
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ /dev/null
@@ -1,84 +0,0 @@
-nextflow_process {
-
-    name "Test Process MULTIQC"
-    script "../main.nf"
-    process "MULTIQC"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "multiqc"
-
-    test("sarscov2 single-end [fastqc]") {
-
-        when {
-            process {
-                """
-                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
-                input[1] = []
-                input[2] = []
-                input[3] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
-                { assert process.out.data[0] ==~ ".*/multiqc_data" },
-                { assert snapshot(process.out.versions).match("multiqc_versions_single") }
-            )
-        }
-
-    }
-
-    test("sarscov2 single-end [fastqc] [config]") {
-
-        when {
-            process {
-                """
-                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
-                input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
-                input[2] = []
-                input[3] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
-                { assert process.out.data[0] ==~ ".*/multiqc_data" },
-                { assert snapshot(process.out.versions).match("multiqc_versions_config") }
-            )
-        }
-    }
-
-    test("sarscov2 single-end [fastqc] - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
-                input[1] = []
-                input[2] = []
-                input[3] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out.report.collect { file(it).getName() } +
-                                process.out.data.collect { file(it).getName() } +
-                                process.out.plots.collect { file(it).getName() } +
-                                process.out.versions ).match("multiqc_stub") }
-            )
-        }
-
-    }
-}
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
deleted file mode 100644
index bfebd802..00000000
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ /dev/null
@@ -1,41 +0,0 @@
-{
-    "multiqc_versions_single": {
-        "content": [
-            [
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-29T08:48:55.657331"
-    },
-    "multiqc_stub": {
-        "content": [
-            [
-                "multiqc_report.html",
-                "multiqc_data",
-                "multiqc_plots",
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-29T08:49:49.071937"
-    },
-    "multiqc_versions_config": {
-        "content": [
-            [
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-29T08:49:25.457567"
-    }
-}
\ No newline at end of file

From e9a4637f58e08146fe35016c15e7d3ac7c20f7e5 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:14:04 +1200
Subject: [PATCH 08/76] Updated modules json

---
 modules.json | 130 +++++++++++++--------------------------------------
 1 file changed, 33 insertions(+), 97 deletions(-)

diff --git a/modules.json b/modules.json
index 2428508b..a4a1f973 100644
--- a/modules.json
+++ b/modules.json
@@ -8,100 +8,72 @@
                     "bwa/index": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fastq_bwa_mem_samblaster"
-                        ]
+                        "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "bwa/mem": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fastq_bwa_mem_samblaster"
-                        ]
+                        "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "cat/cat": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "custom/checkgff3fastacorrespondence": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "gff3_validate"
-                        ]
+                        "installed_by": ["gff3_validate"]
                     },
                     "custom/restoregffids": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "custom/shortenfastaids": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "gt/gff3": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "gff3_validate"
-                        ]
+                        "installed_by": ["gff3_validate"]
                     },
                     "gt/gff3validator": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "gff3_validate"
-                        ]
+                        "installed_by": ["gff3_validate"]
                     },
                     "gt/stat": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ltrfinder": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "ltrharvest": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "ltrretriever/lai": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "ltrretriever/ltrretriever": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fasta_ltrretriever_lai"
-                        ]
+                        "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "samblaster": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "fastq_bwa_mem_samblaster"
-                        ]
+                        "installed_by": ["fastq_bwa_mem_samblaster"]
                     }
                 }
             },
@@ -110,23 +82,17 @@
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "fastq_bwa_mem_samblaster": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "gff3_validate": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
@@ -137,72 +103,52 @@
                     "fastavalidator": {
                         "branch": "master",
                         "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
-                        "installed_by": [
-                            "fastq_trim_fastp_fastqc"
-                        ]
+                        "installed_by": ["fastq_trim_fastp_fastqc"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
-                        "installed_by": [
-                            "fastq_trim_fastp_fastqc"
-                        ]
+                        "installed_by": ["fastq_trim_fastp_fastqc"]
                     },
                     "gunzip": {
                         "branch": "master",
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "seqkit/seq": {
                         "branch": "master",
                         "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4",
-                        "installed_by": [
-                            "fasta_explore_search_plot_tidk"
-                        ]
+                        "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "seqkit/sort": {
                         "branch": "master",
                         "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d",
-                        "installed_by": [
-                            "fasta_explore_search_plot_tidk"
-                        ]
+                        "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "tidk/explore": {
                         "branch": "master",
                         "git_sha": "fad335b31b32d78f89a8340373d3ccfc0f2b18b5",
-                        "installed_by": [
-                            "fasta_explore_search_plot_tidk"
-                        ]
+                        "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "tidk/plot": {
                         "branch": "master",
                         "git_sha": "d54cd1d823f151854825da8ffe7289bcbf77206b",
-                        "installed_by": [
-                            "fasta_explore_search_plot_tidk"
-                        ]
+                        "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "tidk/search": {
                         "branch": "master",
                         "git_sha": "669a329f4aa37f5b7f03776c2ed1cd0ef122c626",
-                        "installed_by": [
-                            "fasta_explore_search_plot_tidk"
-                        ]
+                        "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -211,40 +157,30 @@
                     "fasta_explore_search_plot_tidk": {
                         "branch": "master",
                         "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "fastq_trim_fastp_fastqc": {
                         "branch": "master",
                         "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfvalidation_plugin": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From cdeae208944060dc7907b480c7f82a6b69e55cc5 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:21:29 +1200
Subject: [PATCH 09/76] Restored the original version of
 nf-core/subworkflows/fastq_trim_fastp_fastqc

---
 CHANGELOG.md                                        |  3 ++-
 .../nf-core/fastq_trim_fastp_fastqc/main.nf         | 13 +++++--------
 tests/stub/stub.config                              |  2 ++
 3 files changed, 9 insertions(+), 9 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5c8791d1..78fa449e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0 - [22-April-2024]
+## 2.0.0 - [23-April-2024]
 
 1. Updated nf-core/template to 2.13.2.dev0
 
@@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### `Fixed`
 
 1. Fixed a bug which caused NCBI_FCS_GX to not resume
+2. Restored the original version of `nf-core/subworkflows/fastq_trim_fastp_fastqc`
 
 ### `Dependencies`
 
diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf
index 39a086ad..4f1c84fc 100644
--- a/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf
+++ b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf
@@ -68,14 +68,11 @@ workflow FASTQ_TRIM_FASTP_FASTQC {
         //
         ch_trim_reads
             .join(ch_trim_json)
-            .map { meta, reads, json ->
-                if (json.text.readLines().size < 1) {
-                    return [ meta, reads ]
-                }
-
-                if (getFastpReadsAfterFiltering(json) > 0) {
-                    [ meta, reads ]
-                }
+            .map {
+                meta, reads, json ->
+                    if (getFastpReadsAfterFiltering(json) > 0) {
+                        [ meta, reads ]
+                    }
             }
             .set { ch_trim_reads }
 
diff --git a/tests/stub/stub.config b/tests/stub/stub.config
index 4f753187..0eb35b5c 100644
--- a/tests/stub/stub.config
+++ b/tests/stub/stub.config
@@ -24,6 +24,8 @@ params {
     kraken2_db_path             = 'tests/stub/kraken2/k2_minusb_20231009.tar.gz'
 
     hic                         = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz'
+    hic_skip_fastp              = true
+    hic_skip_fastqc             = true
 
     synteny_skip                = true // GitHub action runner runs out of memory
     synteny_xref_assemblies     = 'assets/xrefsheet.csv'

From 0da90dd1f5178ea1463317cec15c4a26255a5757 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:38:27 +1200
Subject: [PATCH 10/76] Fixed linting issues

---
 .nf-core.yml         | 2 ++
 nextflow_schema.json | 6 ++++++
 2 files changed, 8 insertions(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index 71f34ac4..c00c6830 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -13,11 +13,13 @@ lint:
     - docs/images/nf-core-assemblyqc_logo_light.png
     - docs/images/nf-core-assemblyqc_logo_dark.png
     - .github/ISSUE_TEMPLATE/bug_report.yml
+    - docs/README.md
   multiqc_config:
     - report_comment
   nextflow_config:
     - manifest.name
     - manifest.homePage
+  template_strings: False
 nf_core_version: 2.13.2.dev0
 repository_type: pipeline
 template:
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 6f4e61ed..96e5b50d 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -386,6 +386,12 @@
                     "fa_icon": "fas fa-palette",
                     "hidden": true
                 },
+                "monochromeLogs": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-palette",
+                    "description": "Do not use coloured log outputs.",
+                    "hidden": true
+                },
                 "hook_url": {
                     "type": "string",
                     "description": "Incoming hook URL for messaging service",

From 8f5f2bb163c06345149026b7be75978d09f865c6 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:38:56 +1200
Subject: [PATCH 11/76] Fixed linting issues

---
 assets/multiqc_config.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 759adecd..0b995135 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,8 +1,8 @@
 report_comment: >
-  
+
   This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/releases/tag/2.0.0" target="_blank">plant-food-research-open/assemblyqc</a>
   analysis pipeline.
-  
+
 report_section_order:
   "plant-food-research-open-assemblyqc-methods-description":
     order: -1000

From ab869d95c009ff1a7a693f995e8cf92961f7eea7 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:44:14 +1200
Subject: [PATCH 12/76] Removed .github/CONTRIBUTING.md from linting

---
 .nf-core.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index c00c6830..29866681 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -14,6 +14,7 @@ lint:
     - docs/images/nf-core-assemblyqc_logo_dark.png
     - .github/ISSUE_TEMPLATE/bug_report.yml
     - docs/README.md
+    - .github/CONTRIBUTING.md
   multiqc_config:
     - report_comment
   nextflow_config:

From be0a452683a7224dfb7b2b425e00a01fa1ee6b5c Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 15:55:35 +1200
Subject: [PATCH 13/76] Now using git URL for nf-core/modules

---
 modules.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules.json b/modules.json
index a4a1f973..bed29810 100644
--- a/modules.json
+++ b/modules.json
@@ -97,7 +97,7 @@
                 }
             }
         },
-        "https://github.com/nf-core/modules.git": {
+        "git@github.com:nf-core/modules.git": {
             "modules": {
                 "nf-core": {
                     "fastavalidator": {

From 5267bab86c43d2b401abdd10b556f370e01d461a Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 16:02:29 +1200
Subject: [PATCH 14/76] Fixed n-core linting

---
 CHANGELOG.md | 1 +
 modules.json | 4 ++--
 2 files changed, 3 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 78fa449e..b04f13c1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 1. Fixed a bug which caused NCBI_FCS_GX to not resume
 2. Restored the original version of `nf-core/subworkflows/fastq_trim_fastp_fastqc`
+3. Fixed n-core linting
 
 ### `Dependencies`
 
diff --git a/modules.json b/modules.json
index bed29810..09fd711b 100644
--- a/modules.json
+++ b/modules.json
@@ -2,7 +2,7 @@
     "name": "plant-food-research-open/assemblyqc",
     "homePage": "https://github.com/plant-food-research-open/assemblyqc",
     "repos": {
-        "git@github.com:PlantandFoodResearch/nxf-modules.git": {
+        "https://github.com/PlantandFoodResearch/nxf-modules.git": {
             "modules": {
                 "pfr": {
                     "bwa/index": {
@@ -97,7 +97,7 @@
                 }
             }
         },
-        "git@github.com:nf-core/modules.git": {
+        "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
                     "fastavalidator": {

From 26cf668a6ba5e198c530e59f104e9b6c8ce4fffe Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 16:22:36 +1200
Subject: [PATCH 15/76] Template update for nf-core/tools version 2.13.1

---
 .github/PULL_REQUEST_TEMPLATE.md              |  2 +-
 .github/workflows/ci.yml                      |  2 +-
 .github/workflows/download_pipeline.yml       | 22 ++-------
 .github/workflows/fix-linting.yml             |  4 +-
 .github/workflows/linting.yml                 | 12 ++---
 .github/workflows/linting_comment.yml         |  2 +-
 .github/workflows/release-announcements.yml   |  6 +--
 .nf-core.yml                                  |  1 -
 .pre-commit-config.yaml                       |  3 --
 README.md                                     |  2 +-
 conf/base.config                              |  3 ++
 conf/modules.config                           |  8 ++++
 nextflow.config                               |  3 +-
 pyproject.toml                                | 15 ++++++
 .../utils_nfcore_assemblyqc_pipeline/main.nf  |  4 --
 workflows/assemblyqc.nf                       | 46 +++++--------------
 16 files changed, 59 insertions(+), 76 deletions(-)
 create mode 100644 pyproject.toml

diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 86930edb..12f22500 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/plant-food-r
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md)
 - [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nf-test test tests/ --verbose --profile +docker`).
+- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`).
 - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index ce5b32ae..bd73a829 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -31,7 +31,7 @@ jobs:
         uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v2
+        uses: nf-core/setup-nextflow@v1
         with:
           version: "${{ matrix.NXF_VER }}"
 
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 2d20d644..08622fd5 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -14,8 +14,6 @@ on:
   pull_request:
     types:
       - opened
-      - edited
-      - synchronize
     branches:
       - master
   pull_request_target:
@@ -30,14 +28,11 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v2
+        uses: nf-core/setup-nextflow@v1
 
-      - name: Disk space cleanup
-        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
-
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
-          python-version: "3.12"
+          python-version: "3.11"
           architecture: "x64"
       - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
         with:
@@ -70,17 +65,8 @@ jobs:
       - name: Inspect download
         run: tree ./${{ env.REPOTITLE_LOWERCASE }}
 
-      - name: Run the downloaded pipeline (stub)
-        id: stub_run_pipeline
-        continue-on-error: true
+      - name: Run the downloaded pipeline
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
-      - name: Run the downloaded pipeline (stub run not supported)
-        id: run_pipeline
-        if: ${{ job.steps.stub_run_pipeline.status == failure() }}
-        env:
-          NXF_SINGULARITY_CACHEDIR: ./
-          NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 52217b84..80625a8f 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -32,9 +32,9 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       # Install and run pre-commit
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
-          python-version: "3.12"
+          python-version: 3.11
 
       - name: Install pre-commit
         run: pip install pre-commit
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index a225c816..073e1876 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -16,10 +16,10 @@ jobs:
     steps:
       - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
-      - name: Set up Python 3.12
-        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - name: Set up Python 3.11
+        uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
-          python-version: "3.12"
+          python-version: 3.11
           cache: "pip"
 
       - name: Install pre-commit
@@ -35,11 +35,11 @@ jobs:
         uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v2
+        uses: nf-core/setup-nextflow@v1
 
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
-          python-version: "3.12"
+          python-version: "3.11"
           architecture: "x64"
 
       - name: Install dependencies
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 40acc23f..b706875f 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
+        uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 03ecfcf7..d468aeaa 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -12,7 +12,7 @@ jobs:
       - name: get topics and convert to hashtags
         id: get_topics
         run: |
-          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')"  >> $GITHUB_OUTPUT
+          curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT
 
       - uses: rzr/fediverse-action@master
         with:
@@ -25,13 +25,13 @@ jobs:
 
             Please see the changelog: ${{ github.event.release.html_url }}
 
-            ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
+            ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics
 
   send-tweet:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
           python-version: "3.10"
       - name: Install dependencies
diff --git a/.nf-core.yml b/.nf-core.yml
index 71f34ac4..61d19b4d 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -18,7 +18,6 @@ lint:
   nextflow_config:
     - manifest.name
     - manifest.homePage
-nf_core_version: 2.13.2.dev0
 repository_type: pipeline
 template:
   prefix: plant-food-research-open
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 4dc0f1dc..af57081f 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -3,9 +3,6 @@ repos:
     rev: "v3.1.0"
     hooks:
       - id: prettier
-        additional_dependencies:
-          - prettier@3.2.5
-
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
     rev: "2.7.3"
     hooks:
diff --git a/README.md b/README.md
index 5ea7ea72..19194afb 100644
--- a/README.md
+++ b/README.md
@@ -6,7 +6,7 @@
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
-[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
+[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
 
 ## Introduction
 
diff --git a/conf/base.config b/conf/base.config
index faaec489..fad3aa91 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -59,4 +59,7 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
+    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
+        cache = false
+    }
 }
diff --git a/conf/modules.config b/conf/modules.config
index d203d2b6..e3ea8fa6 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -22,6 +22,14 @@ process {
         ext.args = '--quiet'
     }
 
+    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
+        publishDir = [
+            path: { "${params.outdir}/pipeline_info" },
+            mode: params.publish_dir_mode,
+            pattern: '*_versions.yml'
+        ]
+    }
+
     withName: 'MULTIQC' {
         ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
diff --git a/nextflow.config b/nextflow.config
index c89ce932..cbc0aa8d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -15,7 +15,8 @@ params {
     // References
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
-    igenomes_ignore            = false// MultiQC options
+    igenomes_ignore            = false
+    fasta                      = null// MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     multiqc_logo               = null
diff --git a/pyproject.toml b/pyproject.toml
new file mode 100644
index 00000000..56110621
--- /dev/null
+++ b/pyproject.toml
@@ -0,0 +1,15 @@
+# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff.
+# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
+[tool.ruff]
+line-length = 120
+target-version = "py38"
+cache-dir = "~/.cache/ruff"
+
+[tool.ruff.lint]
+select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
+
+[tool.ruff.lint.isort]
+known-first-party = ["nf_core"]
+
+[tool.ruff.lint.per-file-ignores]
+"__init__.py" = ["E402", "F401"]
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index ac17938f..0e9efa87 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -140,10 +140,6 @@ workflow PIPELINE_COMPLETION {
             imNotification(summary_params, hook_url)
         }
     }
-
-    workflow.onError {
-        log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting"
-    }
 }
 
 /*
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 42da5dd5..cd30a061 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -40,44 +40,22 @@ workflow ASSEMBLYQC {
     // Collate and save software versions
     //
     softwareVersionsToYAML(ch_versions)
-        .collectFile(
-            storeDir: "${params.outdir}/pipeline_info",
-            name: 'nf_core_pipeline_software_mqc_versions.yml',
-            sort: true,
-            newLine: true
-        ).set { ch_collated_versions }
+        .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true)
+        .set { ch_collated_versions }
 
     //
     // MODULE: MultiQC
     //
-    ch_multiqc_config        = Channel.fromPath(
-        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    ch_multiqc_custom_config = params.multiqc_config ?
-        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
-        Channel.empty()
-    ch_multiqc_logo          = params.multiqc_logo ?
-        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-        Channel.empty()
-
-    summary_params      = paramsSummaryMap(
-        workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
-
-    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
-        file(params.multiqc_methods_description, checkIfExists: true) :
-        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(
-        methodsDescriptionText(ch_multiqc_custom_methods_description))
-
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_methods_description.collectFile(
-            name: 'methods_description_mqc.yaml',
-            sort: false
-        )
-    )
+    ch_multiqc_config                     = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config              = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
+    ch_multiqc_logo                       = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty()
+    summary_params                        = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary                   = Channel.value(paramsSummaryMultiqc(summary_params))
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description))
+    ch_multiqc_files                      = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files                      = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files                      = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false))
 
     MULTIQC (
         ch_multiqc_files.collect(),

From e1ca2d7ff20d59ca38b7f81fb41ed9ab4f07c79c Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 17:20:16 +1200
Subject: [PATCH 16/76] Added pfr/modules key

---
 .github/workflows/linting.yml | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 6324793c..d2b8cea3 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -52,7 +52,10 @@ jobs:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-        run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
+        run: |
+          eval `ssh-agent -s`
+          ssh-add - <<< '${{ secrets.PFR_NXF_MODULES_SSH_KEY }}'
+          nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
       - name: Save PR number
         if: ${{ always() }}

From d36c50aff6a16691d86de22fdbebba519a7065d0 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 18:11:41 +1200
Subject: [PATCH 17/76] Updated linting checks

---
 .github/workflows/linting.yml |  19 +++++-
 .nf-core.yml                  |   4 +-
 assets/multiqc_config.yml     |  16 -----
 docs/parameters.md            | 119 +++++++++++++++++-----------------
 4 files changed, 77 insertions(+), 81 deletions(-)
 delete mode 100644 assets/multiqc_config.yml

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index d2b8cea3..b4aba658 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -53,9 +53,22 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
         run: |
-          eval `ssh-agent -s`
-          ssh-add - <<< '${{ secrets.PFR_NXF_MODULES_SSH_KEY }}'
-          nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
+          nf-core -l lint_log.txt lint \
+            --dir ${GITHUB_WORKSPACE} \
+            --markdown lint_results.md \
+            --key actions_ci \
+            --key actions_schema_validation \
+            --key files_exist \
+            --key files_unchanged \
+            --key merge_markers \
+            --key nextflow_config \
+            --key pipeline_name_conventions \
+            --key pipeline_todos \
+            --key readme \
+            --key schema_description \
+            --key schema_lint \
+            --key schema_params \
+            --key system_exit
 
       - name: Save PR number
         if: ${{ always() }}
diff --git a/.nf-core.yml b/.nf-core.yml
index a2b94011..42acee5a 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -15,12 +15,10 @@ lint:
     - .github/ISSUE_TEMPLATE/bug_report.yml
     - docs/README.md
     - .github/CONTRIBUTING.md
-  multiqc_config:
-    - report_comment
+    - .github/workflows/linting.yml
   nextflow_config:
     - manifest.name
     - manifest.homePage
-  template_strings: False
 repository_type: pipeline
 template:
   prefix: plant-food-research-open
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
deleted file mode 100644
index 0b995135..00000000
--- a/assets/multiqc_config.yml
+++ /dev/null
@@ -1,16 +0,0 @@
-report_comment: >
-
-  This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/releases/tag/2.0.0" target="_blank">plant-food-research-open/assemblyqc</a>
-  analysis pipeline.
-
-report_section_order:
-  "plant-food-research-open-assemblyqc-methods-description":
-    order: -1000
-  software_versions:
-    order: -1001
-  "plant-food-research-open-assemblyqc-summary":
-    order: -1002
-
-export_plots: true
-
-disable_version_detection: true
diff --git a/docs/parameters.md b/docs/parameters.md
index 5be39404..f02e854a 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -4,36 +4,36 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## Input/output options
 
-| Parameter | Description                                                                                                                                                                        | Type     | Default   | Required | Hidden |
-| --------- | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | --------- | -------- | ------ |
-| `input`   | Input assembly sheet in CSV format <details><summary>Help</summary><small>FASTA and other associated files for input assemblies provided as a formatted CSV file</small></details> |
-| `outdir`  | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.                                                           | `string` | ./results | True     |        |
-| `email`   | Email address for completion summary. <details><summary>Help</summary><small>Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you |
+| Parameter | Description                                                                                                                                                                                                                                                                                                                                                 | Type     | Default   | Required | Hidden |
+| --------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | --------- | -------- | ------ |
+| `input`   | Input assembly sheet in CSV format <details><summary>Help</summary><small>FASTA and other associated files for input assemblies provided as a formatted CSV file</small></details>                                                                                                                                                                          | `string` |           | True     |        |
+| `outdir`  | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.                                                                                                                                                                                                                                    | `string` | ./results | True     |        |
+| `email`   | Email address for completion summary. <details><summary>Help</summary><small>Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.</small></details> | `string` |           |          |        |
 
 ## General stats options
 
-| Parameter                    | Description                                                                                                                                                    | Type | Default | Required | Hidden |
-| ---------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------- | ---- | ------- | -------- | ------ |
-| `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size <details><summary>Help</summary><small>This number is used to split the scaffolds into contigs to compute contig-r |
+| Parameter                    | Description                                                                                                                                                                                                                                                                                                          | Type      | Default | Required | Hidden |
+| ---------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size <details><summary>Help</summary><small>This number is used to split the scaffolds into contigs to compute contig-related stats such as the number of contigs, N50, etc. NCBI recommendation <https://www.ncbi.nlm.nih.gov/genbank/wgs_gapped/> is 100.</small></details> | `integer` | 100     |          |        |
 
 ## NCBI FCS options
 
-| Parameter                 | Description                                                                                                                                                           | Type      | Default | Required | Hidden |
-| ------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `ncbi_fcs_adaptor_skip`   | Skip NCBI FCS Adaptor checking                                                                                                                                        | `boolean` | True    |          |        |
-| `ncbi_fcs_adaptor_empire` | Empire for NCBI FCS Adaptor checking <details><summary>Help</summary><small>'euk' for eukaryotes, or 'prok' for prokaryotes</small></details>                         | `string`  |         |          |
-| `ncbi_fcs_gx_skip`        | Skip NCBI FCS external organism contamination checking                                                                                                                | `boolean` | True    |          |        |
-| `ncbi_fcs_gx_tax_id`      | Tax ID for NCBI FCS GX <details><summary>Help</summary><small>Get correct tax ID from https://www.ncbi.nlm.nih.gov/taxonomy</small></details>                         | `number`  |         |          |        |
-| `ncbi_fcs_gx_db_path`     | Path to NCBI FCS GX database <details><summary>Help</summary><small>NCBI FCS GX DB path<br><br>Due to enormity of the DB size, the pipeline does NOT download the dat |
+| Parameter                 | Description                                                                                                                                                                                                                                                                                                                         | Type      | Default | Required | Hidden |
+| ------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `ncbi_fcs_adaptor_skip`   | Skip NCBI FCS Adaptor checking                                                                                                                                                                                                                                                                                                      | `boolean` | True    |          |        |
+| `ncbi_fcs_adaptor_empire` | Empire for NCBI FCS Adaptor checking <details><summary>Help</summary><small>'euk' for eukaryotes, or 'prok' for prokaryotes</small></details>                                                                                                                                                                                       | `string`  |         |          |        |
+| `ncbi_fcs_gx_skip`        | Skip NCBI FCS external organism contamination checking                                                                                                                                                                                                                                                                              | `boolean` | True    |          |        |
+| `ncbi_fcs_gx_tax_id`      | Tax ID for NCBI FCS GX <details><summary>Help</summary><small>Get correct tax ID from https://www.ncbi.nlm.nih.gov/taxonomy</small></details>                                                                                                                                                                                       | `number`  |         |          |        |
+| `ncbi_fcs_gx_db_path`     | Path to NCBI FCS GX database <details><summary>Help</summary><small>NCBI FCS GX DB path<br><br>Due to enormity of the DB size, the pipeline does NOT download the data. It must be setup by the user manually before running the pipeline. See instructions for DB setup: https://github.com/ncbi/fcs/wiki/FCS-GX</small></details> | `string`  |         |          |        |
 
 ## BUSCO options
 
-| Parameter                | Description                                                                                                                                                                    | Type      | Default | Required | Hidden |
-| ------------------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
-| `busco_skip`             | Skip BUSCO                                                                                                                                                                     | `boolean` | True    |          |        |
-| `busco_mode`             | BUSCO mode <details><summary>Help</summary><small>'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'pro |
-| `busco_lineage_datasets` | BUSCO lineages <details><summary>Help</summary><small>Each input assembly is assessed against each lineage. It should be provided as a space-separated list of lin             |
-| `busco_download_path`    | Download path for BUSCO <details><summary>Help</summary><small>BUSCO DB download path<br><br>The pipeline automatically downloads the required DB if needed</small></          |
+| Parameter                | Description                                                                                                                                                                                                                              | Type      | Default | Required | Hidden |
+| ------------------------ | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `busco_skip`             | Skip BUSCO                                                                                                                                                                                                                               | `boolean` | True    |          |        |
+| `busco_mode`             | BUSCO mode <details><summary>Help</summary><small>'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'proteins' for annotated gene sets (protein)</small></details> | `string`  |         |          |        |
+| `busco_lineage_datasets` | BUSCO lineages <details><summary>Help</summary><small>Each input assembly is assessed against each lineage. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' </small></details>            | `string`  |         |          |        |
+| `busco_download_path`    | Download path for BUSCO <details><summary>Help</summary><small>BUSCO DB download path<br><br>The pipeline automatically downloads the required DB if needed</small></details>                                                            | `string`  |         |          |        |
 
 ## TIDK options
 
@@ -60,62 +60,63 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 ## HiC options
 
 | Parameter            | Description                                                                                                                                   | Type                      | Default                                           | Required | Hidden |
-| -------------------- | --------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------- | ------------------------------------------------- | -------- | ------ |
-| `hic`                | HiC reads <details><summary>Help</summary><small>Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq | fq).gz'</small></details> | `string`                                          |          |
+| -------------------- | --------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------- | ------------------------------------------------- | -------- | ------ | --- |
+| `hic`                | HiC reads <details><summary>Help</summary><small>Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq | fq).gz'</small></details> | `string`                                          |          |        |     |
 | `hic_skip_fastp`     | Skip HiC read trimming                                                                                                                        | `boolean`                 |                                                   |          |        |
 | `hic_skip_fastqc`    | Skip HiC read QC                                                                                                                              | `boolean`                 |                                                   |          |        |
 | `hic_fastp_ext_args` | Additional parameters for fastp trimming                                                                                                      | `string`                  | --qualified_quality_phred 20 --length_required 50 |          |        |
 
 ## Synteny options
 
-| Parameter                          | Description                                                                                                                                                       | Type      | Default | Required | Hidden |
-| ---------------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `synteny_skip`                     | Skip synteny analysis                                                                                                                                             | `boolean` | True    |          |        |
-| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies                                                                                                       | `boolean` | True    |          |        |
-| `synteny_many_to_many_align`       | Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)                                                                                       | `boolean` |         |          |        |
-| `synteny_max_gap`                  | Alignments within this distance are bundled together                                                                                                              | `integer` | 1000000 |          |        |
-| `synteny_min_bundle_size`          | After bundling, any bundle smaller than this size is filtered out                                                                                                 | `integer` | 1000    |          |        |
-| `synteny_plot_1_vs_all`            | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly                                                | `boolean` | True    |          |        |
-| `synteny_color_by_contig`          | Synteny plot is colored by contig                                                                                                                                 | `boolean` | True    |          |        |
-| `synteny_xref_assemblies`          | Reference assemblies for synteny analysis <details><summary>Help</summary><small>FASTA and synteny label tsv files should be provided in a formatted CSV file </s |
+| Parameter                          | Description                                                                                                                                                                      | Type      | Default | Required | Hidden |
+| ---------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `synteny_skip`                     | Skip synteny analysis                                                                                                                                                            | `boolean` | True    |          |        |
+| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies                                                                                                                      | `boolean` | True    |          |        |
+| `synteny_many_to_many_align`       | Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)                                                                                                      | `boolean` |         |          |        |
+| `synteny_max_gap`                  | Alignments within this distance are bundled together                                                                                                                             | `integer` | 1000000 |          |        |
+| `synteny_min_bundle_size`          | After bundling, any bundle smaller than this size is filtered out                                                                                                                | `integer` | 1000    |          |        |
+| `synteny_plot_1_vs_all`            | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly                                                               | `boolean` | True    |          |        |
+| `synteny_color_by_contig`          | Synteny plot is colored by contig                                                                                                                                                | `boolean` | True    |          |        |
+| `synteny_xref_assemblies`          | Reference assemblies for synteny analysis <details><summary>Help</summary><small>FASTA and synteny label tsv files should be provided in a formatted CSV file </small></details> | `string`  |         |          |        |
 
 ## Max job request options
 
 Set the top limit for requested resources for any single job.
 
-| Parameter    | Description                                                                                                                                                                      | Type | Default | Required | Hidden |
-| ------------ | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ---- | ------- | -------- | ------ |
-| `max_cpus`   | Maximum number of CPUs that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the CPU requirement for each process. Shou |
-| `max_memory` | Maximum amount of memory that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the memory requirement for each proces   |
-| `max_time`   | Maximum amount of time that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the time requirement for each process. Sho |
+| Parameter    | Description                                                                                                                                                                                                                                                                 | Type      | Default | Required | Hidden |
+| ------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `max_cpus`   | Maximum number of CPUs that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`</small></details>                                      | `integer` | 16      |          | True   |
+| `max_memory` | Maximum amount of memory that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`</small></details> | `string`  | 512.GB  |          | True   |
+| `max_time`   | Maximum amount of time that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`</small></details>        | `string`  | 7.day   |          | True   |
 
 ## Institutional config options
 
 Parameters used to describe centralised config profiles. These should not be edited.
 
-| Parameter                    | Description                                                                                                                                                            | Type     | Default | Required | Hidden |
-| ---------------------------- | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | ------- | -------- | ------ |
-| `custom_config_version`      | Git commit id for Institutional configs.                                                                                                                               | `string` | master  |          | True   |
-| `custom_config_base`         | Base directory for Institutional configs. <details><summary>Help</summary><small>If you're running offline, Nextflow will not be able to fetch the institutional confi |
-| `config_profile_name`        | Institutional config name.                                                                                                                                             | `string` |         |          | True   |
-| `config_profile_description` | Institutional config description.                                                                                                                                      | `string` |         |          | True   |
-| `config_profile_contact`     | Institutional config contact information.                                                                                                                              | `string` |         |          | True   |
-| `config_profile_url`         | Institutional config URL link.                                                                                                                                         | `string` |         |          | True   |
+| Parameter                    | Description                                                                                                                                                                                                                                                                                                                                                                                       | Type     | Default                                                  | Required | Hidden |
+| ---------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | -------------------------------------------------------- | -------- | ------ |
+| `custom_config_version`      | Git commit id for Institutional configs.                                                                                                                                                                                                                                                                                                                                                          | `string` | master                                                   |          | True   |
+| `custom_config_base`         | Base directory for Institutional configs. <details><summary>Help</summary><small>If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.</small></details> | `string` | https://raw.githubusercontent.com/nf-core/configs/master |          | True   |
+| `config_profile_name`        | Institutional config name.                                                                                                                                                                                                                                                                                                                                                                        | `string` |                                                          |          | True   |
+| `config_profile_description` | Institutional config description.                                                                                                                                                                                                                                                                                                                                                                 | `string` |                                                          |          | True   |
+| `config_profile_contact`     | Institutional config contact information.                                                                                                                                                                                                                                                                                                                                                         | `string` |                                                          |          | True   |
+| `config_profile_url`         | Institutional config URL link.                                                                                                                                                                                                                                                                                                                                                                    | `string` |                                                          |          | True   |
 
 ## Generic options
 
 Less common options for the pipeline, typically set in a config file.
 
-| Parameter                          | Description                                                                                                                                                                      | Type      | Default | Required | Hidden |
-| ---------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `help`                             | Display help text.                                                                                                                                                               | `boolean` |         |          | True   |
-| `version`                          | Display version and exit.                                                                                                                                                        | `boolean` |         |          | True   |
-| `publish_dir_mode`                 | Method used to save pipeline results to output directory. <details><summary>Help</summary><small>The Nextflow `publishDir` option specifies which intermediate files sho         |
-| `email_on_fail`                    | Email address for completion summary, only when pipeline fails. <details><summary>Help</summary><small>An email address to send a summary email to when the pipeline is com      |
-| `plaintext_email`                  | Send plain-text email instead of HTML.                                                                                                                                           | `boolean` |         |          | True   |
-| `monochrome_logs`                  | Do not use coloured log outputs.                                                                                                                                                 | `boolean` |         |          | True   |
-| `hook_url`                         | Incoming hook URL for messaging service <details><summary>Help</summary><small>Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.</small></de |
-| `validate_params`                  | Boolean whether to validate parameters against the schema at runtime                                                                                                             | `boolean` | True    |          | True   |
-| `validationShowHiddenParams`       | Show all params when using `--help` <details><summary>Help</summary><small>By default, parameters set as _hidden_ in the schema are not shown on the command l                   |
-| `validationFailUnrecognisedParams` | Validation of parameters fails when an unrecognised parameter is found. <details><summary>Help</summary><small>By default, when an unrecognised paramete                         |
-| `validationLenientMode`            | Validation of parameters in lenient more. <details><summary>Help</summary><small>Allows string values that are parseable as numbers or booleans. For further inform              |
+| Parameter                          | Description                                                                                                                                                                                                                                                                                                                                                                                                  | Type      | Default | Required | Hidden |
+| ---------------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
+| `help`                             | Display help text.                                                                                                                                                                                                                                                                                                                                                                                           | `boolean` |         |          | True   |
+| `version`                          | Display version and exit.                                                                                                                                                                                                                                                                                                                                                                                    | `boolean` |         |          | True   |
+| `publish_dir_mode`                 | Method used to save pipeline results to output directory. <details><summary>Help</summary><small>The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.</small></details> | `string`  | copy    |          | True   |
+| `email_on_fail`                    | Email address for completion summary, only when pipeline fails. <details><summary>Help</summary><small>An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.</small></details>                                                                                                                                                  | `string`  |         |          | True   |
+| `plaintext_email`                  | Send plain-text email instead of HTML.                                                                                                                                                                                                                                                                                                                                                                       | `boolean` |         |          | True   |
+| `monochrome_logs`                  | Do not use coloured log outputs.                                                                                                                                                                                                                                                                                                                                                                             | `boolean` |         |          | True   |
+| `monochromeLogs`                   | Do not use coloured log outputs.                                                                                                                                                                                                                                                                                                                                                                             | `boolean` |         |          | True   |
+| `hook_url`                         | Incoming hook URL for messaging service <details><summary>Help</summary><small>Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.</small></details>                                                                                                                                                                                                                       | `string`  |         |          | True   |
+| `validate_params`                  | Boolean whether to validate parameters against the schema at runtime                                                                                                                                                                                                                                                                                                                                         | `boolean` | True    |          | True   |
+| `validationShowHiddenParams`       | Show all params when using `--help` <details><summary>Help</summary><small>By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters.</small></details>                                                                                                                    | `boolean` |         |          | True   |
+| `validationFailUnrecognisedParams` | Validation of parameters fails when an unrecognised parameter is found. <details><summary>Help</summary><small>By default, when an unrecognised parameter is found, it returns a warinig.</small></details>                                                                                                                                                                                                  | `boolean` |         |          | True   |
+| `validationLenientMode`            | Validation of parameters in lenient more. <details><summary>Help</summary><small>Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode).</small></details>                                                                                                                                    | `boolean` |         |          | True   |

From c21e2688ebf1304b60fb7f882cdc6aacb59096c5 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 23 Apr 2024 18:19:13 +1200
Subject: [PATCH 18/76] Updated tower.yml

---
 CHANGELOG.md | 1 +
 tower.yml    | 6 ++----
 2 files changed, 3 insertions(+), 4 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4686763b..9888b17b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 1. Fixed a bug which caused NCBI_FCS_GX to not resume
 2. Restored the original version of `nf-core/subworkflows/fastq_trim_fastp_fastqc`
 3. Fixed n-core linting
+4. Updated `tower.yml`
 
 ### `Dependencies`
 
diff --git a/tower.yml b/tower.yml
index 787aedfe..96116b57 100644
--- a/tower.yml
+++ b/tower.yml
@@ -1,5 +1,3 @@
 reports:
-  multiqc_report.html:
-    display: "MultiQC HTML report"
-  samplesheet.csv:
-    display: "Auto-created samplesheet with collated metadata and FASTQ paths"
+  report.html:
+    display: "AssemblyQC report"

From 22bab61cc1dc175fcdee61cc4a06f450f5722285 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 29 Apr 2024 15:57:00 +1200
Subject: [PATCH 19/76] Updated LICENSE

---
 .nf-core.yml | 1 +
 CHANGELOG.md | 6 +++---
 LICENSE      | 2 +-
 3 files changed, 5 insertions(+), 4 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 42acee5a..b8a0eff0 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -16,6 +16,7 @@ lint:
     - docs/README.md
     - .github/CONTRIBUTING.md
     - .github/workflows/linting.yml
+    - LICENSE
   nextflow_config:
     - manifest.name
     - manifest.homePage
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9888b17b..26bb97f9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,13 +3,12 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0 - [23-April-2024]
-
-1. Updated nf-core/template to 2.13.1
+## 2.0.0 - [29-April-2024]
 
 ### `Added`
 
 1. Updated modules and sub-workflows
+2. Updated nf-core/template to 2.13.1
 
 ### `Fixed`
 
@@ -17,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 2. Restored the original version of `nf-core/subworkflows/fastq_trim_fastp_fastqc`
 3. Fixed n-core linting
 4. Updated `tower.yml`
+5. Updated LICENSE copyright to Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited
 
 ### `Dependencies`
 
diff --git a/LICENSE b/LICENSE
index 96e3eb88..2ef2204c 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy
+Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal

From f7d5dc363ac43dc92cf91bf5dcc29e9f1e21f178 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 30 Apr 2024 14:35:51 +1200
Subject: [PATCH 20/76] Removed release-announcements GitHub workflow and added
 a list of nf-core contributors

---
 .github/workflows/release-announcements.yml | 75 ---------------------
 .nf-core.yml                                |  1 +
 CHANGELOG.md                                |  4 +-
 README.md                                   | 21 +++++-
 docs/contributors.sh                        |  5 ++
 5 files changed, 29 insertions(+), 77 deletions(-)
 delete mode 100644 .github/workflows/release-announcements.yml
 create mode 100755 docs/contributors.sh

diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
deleted file mode 100644
index d468aeaa..00000000
--- a/.github/workflows/release-announcements.yml
+++ /dev/null
@@ -1,75 +0,0 @@
-name: release-announcements
-# Automatic release toot and tweet anouncements
-on:
-  release:
-    types: [published]
-  workflow_dispatch:
-
-jobs:
-  toot:
-    runs-on: ubuntu-latest
-    steps:
-      - name: get topics and convert to hashtags
-        id: get_topics
-        run: |
-          curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT
-
-      - uses: rzr/fediverse-action@master
-        with:
-          access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
-          host: "mstdn.science" # custom host if not "mastodon.social" (default)
-          # GitHub event payload
-          # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release
-          message: |
-            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
-
-            Please see the changelog: ${{ github.event.release.html_url }}
-
-            ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics
-
-  send-tweet:
-    runs-on: ubuntu-latest
-
-    steps:
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
-        with:
-          python-version: "3.10"
-      - name: Install dependencies
-        run: pip install tweepy==4.14.0
-      - name: Send tweet
-        shell: python
-        run: |
-          import os
-          import tweepy
-
-          client = tweepy.Client(
-              access_token=os.getenv("TWITTER_ACCESS_TOKEN"),
-              access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"),
-              consumer_key=os.getenv("TWITTER_CONSUMER_KEY"),
-              consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"),
-          )
-          tweet = os.getenv("TWEET")
-          client.create_tweet(text=tweet)
-        env:
-          TWEET: |
-            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
-
-            Please see the changelog: ${{ github.event.release.html_url }}
-          TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }}
-          TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }}
-          TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }}
-          TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }}
-
-  bsky-post:
-    runs-on: ubuntu-latest
-    steps:
-      - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0
-        with:
-          post: |
-            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
-
-            Please see the changelog: ${{ github.event.release.html_url }}
-        env:
-          BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }}
-          BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }}
-          #
diff --git a/.nf-core.yml b/.nf-core.yml
index b8a0eff0..5d078e43 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -20,6 +20,7 @@ lint:
   nextflow_config:
     - manifest.name
     - manifest.homePage
+  template_strings: False
 repository_type: pipeline
 template:
   prefix: plant-food-research-open
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 26bb97f9..5b22f9ef 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,12 +3,14 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0 - [29-April-2024]
+## 2.0.0 - [30-April-2024]
 
 ### `Added`
 
 1. Updated modules and sub-workflows
 2. Updated nf-core/template to 2.13.1
+3. Removed release-announcements GitHub workflow
+4. Added a list of nf-core contributors
 
 ### `Fixed`
 
diff --git a/README.md b/README.md
index 39c6bfc3..5332e400 100644
--- a/README.md
+++ b/README.md
@@ -90,7 +90,7 @@ nextflow run plant-food-research-open/assemblyqc \
 > Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
 > see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
 
-### Quick Start for Plant&Food Users
+### Plant&Food Users
 
 Download the pipeline to your `/workspace/$USER` folder. Change the parameters defined in the [pfr/params.json](./pfr/params.json) file. Submit the pipeline to SLURM for execution.
 
@@ -112,6 +112,25 @@ We thank the following people for their extensive assistance in the development
 - Susan Thomson [@cflsjt](https://github.com/cflsjt)
 - Ting-Hsuan Chen [@ting-hsuan-chen](https://github.com/ting-hsuan-chen)
 
+The pipeline uses nf-core modules contributed by following authors.
+
+<a href="https://github.com/adamrtalbot"><img src="https://github.com/adamrtalbot.png" width="50" height="50"></a>
+<a href="https://github.com/drpatelh"><img src="https://github.com/drpatelh.png" width="50" height="50"></a>
+<a href="https://github.com/erikrikarddaniel"><img src="https://github.com/erikrikarddaniel.png" width="50" height="50"></a>
+<a href="https://github.com/ewels"><img src="https://github.com/ewels.png" width="50" height="50"></a>
+<a href="https://github.com/felixkrueger"><img src="https://github.com/felixkrueger.png" width="50" height="50"></a>
+<a href="https://github.com/friederikehanssen"><img src="https://github.com/friederikehanssen.png" width="50" height="50"></a>
+<a href="https://github.com/gallvp"><img src="https://github.com/gallvp.png" width="50" height="50"></a>
+<a href="https://github.com/grst"><img src="https://github.com/grst.png" width="50" height="50"></a>
+<a href="https://github.com/jeremy1805"><img src="https://github.com/jeremy1805.png" width="50" height="50"></a>
+<a href="https://github.com/jfy133"><img src="https://github.com/jfy133.png" width="50" height="50"></a>
+<a href="https://github.com/joon-klaps"><img src="https://github.com/joon-klaps.png" width="50" height="50"></a>
+<a href="https://github.com/joseespinosa"><img src="https://github.com/joseespinosa.png" width="50" height="50"></a>
+<a href="https://github.com/kevinmenden"><img src="https://github.com/kevinmenden.png" width="50" height="50"></a>
+<a href="https://github.com/lescai"><img src="https://github.com/lescai.png" width="50" height="50"></a>
+<a href="https://github.com/matthdsm"><img src="https://github.com/matthdsm.png" width="50" height="50"></a>
+<a href="https://github.com/maxulysse"><img src="https://github.com/maxulysse.png" width="50" height="50"></a>
+
 ## Contributions and Support
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
diff --git a/docs/contributors.sh b/docs/contributors.sh
new file mode 100755
index 00000000..8dbfc364
--- /dev/null
+++ b/docs/contributors.sh
@@ -0,0 +1,5 @@
+#!/usr/bin/env bash
+
+module_authors=$(find ./modules -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '[-" ]' | tr '[:upper:]' '[:lower:]')
+workflow_authors=$(find ./subworkflows -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '[-" ]' | tr '[:upper:]' '[:lower:]')
+echo -e "${module_authors}\n${workflow_authors}" | sort -V | uniq | sed -n 's|@\(.*\)|<a href="https://github.com/\1"><img src="https://github.com/\1.png" width="50" height="50"></a>|p'

From a537e1cb1e9c3af78d532115e6f7bd02f24a4b3e Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 1 May 2024 13:14:57 +1200
Subject: [PATCH 21/76] RUNASSEMBLYVISUALIZER is now single threaded for
 successful execution on both Linux and MacOS

---
 CHANGELOG.md                           |  4 +++-
 conf/test_full.config                  |  2 +-
 local_assemblyqc                       | 27 ++++++++++++++++++++++++++
 modules/local/runassemblyvisualizer.nf |  4 ++--
 4 files changed, 33 insertions(+), 4 deletions(-)
 create mode 100755 local_assemblyqc

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5b22f9ef..6af70942 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0 - [30-April-2024]
+## 2.0.0 - [01-May-2024]
 
 ### `Added`
 
@@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 2. Updated nf-core/template to 2.13.1
 3. Removed release-announcements GitHub workflow
 4. Added a list of nf-core contributors
+5. Added a launcher script for local testing `local_assemblyqc`
 
 ### `Fixed`
 
@@ -19,6 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 3. Fixed n-core linting
 4. Updated `tower.yml`
 5. Updated LICENSE copyright to Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited
+6. `RUNASSEMBLYVISUALIZER` is now single threaded for successful execution on both Linux and MacOS
 
 ### `Dependencies`
 
diff --git a/conf/test_full.config b/conf/test_full.config
index 4c4b5151..c93ba771 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -20,7 +20,7 @@ params {
     ncbi_fcs_adaptor_empire     = 'euk'
 
     ncbi_fcs_gx_skip            = true // Skipping this step as the dataset is humengous (500 GB). Please download the dataset manually
-    // ncbi_fcs_gx_tax_id       = 35717
+    ncbi_fcs_gx_tax_id          = 35717
     // ncbi_fcs_gx_db_path      = 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2023-01-24'
 
     busco_skip                  = false
diff --git a/local_assemblyqc b/local_assemblyqc
new file mode 100755
index 00000000..f8dd29a2
--- /dev/null
+++ b/local_assemblyqc
@@ -0,0 +1,27 @@
+#!/usr/bin/env bash
+
+NO_FORMAT="\033[0m"
+C_RED="\033[38;5;9m"
+F_BOLD="\033[1m"
+
+[[ $1 == '-stub' ]] \
+    && stub='-stub' \
+    || stub=''
+
+[[ $1 == '-stub' ]] \
+    && echo 'Executing with -stub' \
+    || echo -e "${C_RED}${F_BOLD}Executing without -stub${NO_FORMAT}"
+
+nextflow run \
+    main.nf \
+    -profile docker,test_full \
+    -resume \
+    $stub \
+    --max_cpus 8 \
+    --max_memory '32.GB' \
+    --ncbi_fcs_gx_skip false \
+    --ncbi_fcs_gx_db_path ../dbs/gxdb/test \
+    --busco_download_path ../dbs/busco \
+    --kraken2_skip false \
+    --kraken2_db_path ../dbs/kraken2db/k2_minusb \
+    --hic "$(realpath ../test-data/assemblyqc/hic)/SRR8238190_{1,2}.fastq.gz"
diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf
index 7b628d08..8a0d6713 100644
--- a/modules/local/runassemblyvisualizer.nf
+++ b/modules/local/runassemblyvisualizer.nf
@@ -1,6 +1,6 @@
 process RUNASSEMBLYVISUALIZER {
     tag "$sample_id_on_tag"
-    label 'process_medium'
+    label 'process_single'
 
     container "docker.io/gallvp/3d-dna:63029aa"
 
@@ -15,12 +15,12 @@ process RUNASSEMBLYVISUALIZER {
     task.ext.when == null || task.ext.when
 
     script:
-    // -p true/false    Use GNU Parallel to speed up computation (default is true).
     """
     assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g')
     file_name="${agp_assembly_file}"
 
     /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\
+        -p false \\
         $agp_assembly_file $sorted_links_txt_file
 
     mv "\${file_name%.*}.hic" "\${assembly_tag}.hic"

From 4c5998be5dc5790901cba379d1d77260a3365ff7 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 1 May 2024 14:39:24 +1200
Subject: [PATCH 22/76] Added a custom BUNDLELINKS module which respects
 direction when bundling dnadiff links

---
 CHANGELOG.md                        |   3 +
 bin/bundlelinks.py                  | 267 ++++++++++++++++++++++++++++
 modules/local/assembly2bedpe.nf     |   2 +-
 modules/local/bundlelinks.nf        |  47 +++++
 modules/local/circos_bundlelinks.nf |  39 ----
 modules/local/colourbundlelinks.nf  |   2 +-
 modules/local/createreport.nf       |   2 +-
 modules/local/dnadiff.nf            |   5 +-
 modules/local/hic2html.nf           |   2 +-
 modules/local/relabelbundlelinks.nf |   2 +-
 modules/local/relabelfastalength.nf |   2 +-
 subworkflows/local/fasta_synteny.nf |  10 +-
 12 files changed, 331 insertions(+), 52 deletions(-)
 create mode 100755 bin/bundlelinks.py
 create mode 100644 modules/local/bundlelinks.nf
 delete mode 100644 modules/local/circos_bundlelinks.nf

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 6af70942..a47c6aa6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -12,6 +12,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 3. Removed release-announcements GitHub workflow
 4. Added a list of nf-core contributors
 5. Added a launcher script for local testing `local_assemblyqc`
+6. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links
 
 ### `Fixed`
 
@@ -29,6 +30,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### `Deprecated`
 
+1. Removed `CIRCOS_BUNDLELINKS` module
+
 ## 1.4 - [04-Mar-2024]
 
 ### `Added`
diff --git a/bin/bundlelinks.py b/bin/bundlelinks.py
new file mode 100755
index 00000000..1cc28a46
--- /dev/null
+++ b/bin/bundlelinks.py
@@ -0,0 +1,267 @@
+#!/usr/bin/env python3
+
+import argparse
+
+
+def log(verbose, message):
+    if verbose:
+        print(message)
+
+
+def dir_sign(end, start):
+    if abs(int(end) - int(start)) == 0:
+        return 1
+    sign = (int(end) - int(start)) / abs(int(end) - int(start))
+    return sign
+
+
+def is_same_direction(link, bundle, verbose):
+    _, ref_start, ref_end, _, target_start, target_end = link
+    (
+        _,
+        bundle_ref_start,
+        bundle_ref_end,
+        _,
+        bundle_target_start,
+        bundle_target_end,
+    ) = bundle
+
+    link_ref_dir = dir_sign(ref_end, ref_start)
+    link_target_dir = dir_sign(target_end, target_start)
+
+    bundle_ref_dir = dir_sign(bundle_ref_end, bundle_ref_start)
+    bundle_target_dir = dir_sign(bundle_target_end, bundle_target_start)
+
+    log(
+        verbose,
+        f"Compared directions l: {link_ref_dir},{link_target_dir} and b: {bundle_ref_dir},{bundle_target_dir}",
+    )
+
+    if link_ref_dir == bundle_ref_dir and link_target_dir == bundle_target_dir:
+        return True
+
+    return False
+
+
+def within_max_gap(link, bundle, max_gap):
+    _, ref_start, ref_end, _, target_start, target_end = link
+    (
+        _,
+        bundle_ref_start,
+        bundle_ref_end,
+        _,
+        bundle_target_start,
+        bundle_target_end,
+    ) = bundle
+
+    ref_start = int(ref_start)
+    ref_end = int(ref_end)
+    target_start = int(target_start)
+    target_end = int(target_end)
+
+    bundle_ref_start = int(bundle_ref_start)
+    bundle_ref_end = int(bundle_ref_end)
+    bundle_target_start = int(bundle_target_start)
+    bundle_target_end = int(bundle_target_end)
+
+    ref_within_max_gap = (
+        abs(ref_start - bundle_ref_start) <= max_gap
+        or abs(ref_start - bundle_ref_end) <= max_gap
+        or abs(ref_end - bundle_ref_start) <= max_gap
+        or abs(ref_end - bundle_ref_end) <= max_gap
+    )
+
+    target_within_max_gap = (
+        abs(target_start - bundle_target_start) <= max_gap
+        or abs(target_start - bundle_target_end) <= max_gap
+        or abs(target_end - bundle_target_start) <= max_gap
+        or abs(target_end - bundle_target_end) <= max_gap
+    )
+
+    return ref_within_max_gap and target_within_max_gap
+
+
+def get_bundle_directions(bundle):
+    (
+        _,
+        bundle_ref_start,
+        bundle_ref_end,
+        _,
+        bundle_target_start,
+        bundle_target_end,
+    ) = bundle
+
+    return (
+        dir_sign(bundle_ref_end, bundle_ref_start),
+        dir_sign(bundle_target_end, bundle_target_start),
+    )
+
+
+def add_link_to_bundle(link, bundle, verbose):
+    ref_dir, target_dir = get_bundle_directions(bundle)
+
+    log(verbose, f"Bundle directions l: {ref_dir},{target_dir}")
+
+    _, ref_start, ref_end, _, target_start, target_end = link
+
+    ref_start = int(ref_start)
+    ref_end = int(ref_end)
+    target_start = int(target_start)
+    target_end = int(target_end)
+
+    (
+        ref,
+        bundle_ref_start,
+        bundle_ref_end,
+        target,
+        bundle_target_start,
+        bundle_target_end,
+    ) = bundle
+
+    bundle_ref_start = int(bundle_ref_start)
+    bundle_ref_end = int(bundle_ref_end)
+    bundle_target_start = int(bundle_target_start)
+    bundle_target_end = int(bundle_target_end)
+
+    updated_bundle_ref_start = None
+    updated_bundle_ref_end = None
+    updated_bundle_target_start = None
+    updated_bundle_target_end = None
+
+    if ref_dir > 0:
+        updated_bundle_ref_start = min(ref_start, bundle_ref_start)
+        updated_bundle_ref_end = max(ref_end, bundle_ref_end)
+    else:
+        updated_bundle_ref_start = max(ref_start, bundle_ref_start)
+        updated_bundle_ref_end = min(ref_end, bundle_ref_end)
+
+    if target_dir > 0:
+        updated_bundle_target_start = min(target_start, bundle_target_start)
+        updated_bundle_target_end = max(target_end, bundle_target_end)
+    else:
+        updated_bundle_target_start = max(target_start, bundle_target_start)
+        updated_bundle_target_end = min(target_end, bundle_target_end)
+
+    return [
+        ref,
+        updated_bundle_ref_start,
+        updated_bundle_ref_end,
+        target,
+        updated_bundle_target_start,
+        updated_bundle_target_end,
+    ]
+
+
+def bundle_len(bundle):
+    (
+        _,
+        bundle_ref_start,
+        bundle_ref_end,
+        _,
+        bundle_target_start,
+        bundle_target_end,
+    ) = bundle
+
+    return min(
+        abs(int(bundle_ref_end) - int(bundle_ref_start)),
+        abs(int(bundle_target_end) - int(bundle_target_start)),
+    )
+
+
+def bundle_links(input_file, output_file, max_gap, min_bundle_size, verbose):
+    bundles = {}
+    nlinks = {}
+    current_bundle_num = 0
+
+    with open(input_file, "r") as f:
+        for line in f:
+            link = line.strip().split("\t")
+            ref, _, _, target, _, _ = link
+
+            log(verbose, f"Link: {link}")
+
+            link_key = f"{ref}:{target}"
+            possible_bundles_to_add_to = [
+                k for k in bundles.keys() if k.startswith(link_key)
+            ]
+
+            log(verbose, f"Possible bundles are: {possible_bundles_to_add_to}")
+
+            # Check if the current link can be added to an existing bundle
+            link_added = False
+            for k in possible_bundles_to_add_to:
+                bundle = bundles[k]
+
+                log(verbose, f"Checking against bundle: {bundle}")
+
+                if not is_same_direction(link, bundle, verbose):
+                    log(verbose, "Bundle and link have different directions")
+                    continue
+
+                if not within_max_gap(link, bundle, max_gap):
+                    log(verbose, f"Bundle and link are not within {max_gap}")
+                    continue
+
+                # Add link to bundle
+                updated_bundle = add_link_to_bundle(link, bundle, verbose)
+                bundles[k] = updated_bundle
+                link_added = True
+                nlinks[k] += 1
+                log(verbose, f"Added link to bundle")
+                log(verbose, f"Updated bundle: {updated_bundle}")
+                break
+
+            # Create a new bundle for the current link
+            if not link_added:
+                current_bundle_num += 1
+                bundles[link_key + f":{current_bundle_num}"] = link
+                nlinks[link_key + f":{current_bundle_num}"] = 0
+                log(verbose, f"Created bundle: {link}")
+
+    # Filter out bundles smaller than the minimum bundle size
+    filtered_bundles = {
+        key: bundle
+        for key, bundle in bundles.items()
+        if bundle_len(bundle) >= min_bundle_size
+    }
+
+    # Write the filtered bundles to the output file
+    with open(output_file, "w") as f:
+        for k, bundle in filtered_bundles.items():
+            bundle_nlinks = nlinks[k]
+            f.write(
+                "\t".join([str(v) for v in bundle] + [f"nlinks={bundle_nlinks}"]) + "\n"
+            )
+
+
+if __name__ == "__main__":
+    parser = argparse.ArgumentParser(
+        description="Bundle links",
+        epilog="Author: ChatGPT, Usman Rashid",
+    )
+    parser.add_argument("input_file", help="Input TSV file containing links")
+    parser.add_argument("output_file", help="Output TSV file to write bundled links")
+    parser.add_argument(
+        "--max_gap",
+        type=int,
+        default=1_000_000,
+        help="Maximum gap allowed between links for bundling",
+    )
+    parser.add_argument(
+        "--min_bundle_size",
+        type=int,
+        default=1_000,
+        help="Minimum size of a bundle to retain",
+    )
+
+    parser.add_argument("--verbose", help="Print info messages", action="store_true")
+
+    args = parser.parse_args()
+
+    bundle_links(
+        args.input_file,
+        args.output_file,
+        args.max_gap,
+        args.min_bundle_size,
+        args.verbose,
+    )
diff --git a/modules/local/assembly2bedpe.nf b/modules/local/assembly2bedpe.nf
index 48e99f6c..66865ead 100644
--- a/modules/local/assembly2bedpe.nf
+++ b/modules/local/assembly2bedpe.nf
@@ -2,7 +2,7 @@ process ASSEMBLY2BEDPE {
     tag "$sample_id_on_tag"
     label 'process_single'
 
-    container "docker.io/gallvp/python3npkgs:v0.6"
+    container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
     tuple val(sample_id_on_tag), path(agp_assembly_file)
diff --git a/modules/local/bundlelinks.nf b/modules/local/bundlelinks.nf
new file mode 100644
index 00000000..d22cced6
--- /dev/null
+++ b/modules/local/bundlelinks.nf
@@ -0,0 +1,47 @@
+process BUNDLELINKS {
+    tag "${target_on_ref}"
+    label 'process_single'
+
+    container "docker.io/gallvp/python3npkgs:v0.7"
+
+    input:
+    tuple val(target_on_ref), path(coords_file)
+    val max_gap
+    val min_bundle_size
+
+    output:
+    tuple val(target_on_ref), path("*.xcoords.bundle.txt")  , emit: links
+    path "versions.yml"                                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    cat \\
+        $coords_file \\
+        | awk '{print \$12,\$1,\$2,\$13,\$3,\$4}' OFS="\\t" \\
+        > "\$(basename $coords_file).links.txt"
+
+    bundlelinks.py \\
+        --max_gap $max_gap \\
+        --min_bundle_size $min_bundle_size \\
+        "\$(basename $coords_file).links.txt" \\
+        "\$(basename $coords_file).bundle.txt"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | tr -d 'Python[:space:]')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch "\$(basename $coords_file).bundle.txt"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | tr -d 'Python[:space:]')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/circos_bundlelinks.nf b/modules/local/circos_bundlelinks.nf
deleted file mode 100644
index d8f5d9f8..00000000
--- a/modules/local/circos_bundlelinks.nf
+++ /dev/null
@@ -1,39 +0,0 @@
-process CIRCOS_BUNDLELINKS {
-    tag "${target_on_ref}"
-    label 'process_single'
-
-    container "docker.io/gallvp/circos-tools:v0.23-1_ps"
-
-    input:
-    tuple val(target_on_ref), path(coords_file), path(report_file)
-    val max_gap
-    val min_bundle_size
-
-    output:
-    tuple val(target_on_ref), path("*.xcoords.bundle.txt")  , emit: links
-    path "versions.yml"                                     , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def VERSION='24Sep2013'
-    """
-    cat \\
-        $coords_file \\
-        | awk '{print \$12,\$1,\$2,\$13,\$3,\$4}' OFS="\\t" \\
-        > "\$(basename $coords_file).links.txt"
-
-    /usr/share/circos/tools/bundlelinks/bin/bundlelinks \\
-        -links "\$(basename $coords_file).links.txt" \\
-        -max_gap $max_gap \\
-        -min_bundle_size $min_bundle_size \\
-        1> "\$(basename $coords_file).bundle.txt" \\
-        2> bundlelinks.err
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bundlelinks: $VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/colourbundlelinks.nf b/modules/local/colourbundlelinks.nf
index 6d753984..676a8941 100644
--- a/modules/local/colourbundlelinks.nf
+++ b/modules/local/colourbundlelinks.nf
@@ -2,7 +2,7 @@ process COLOURBUNDLELINKS {
     tag "${target_on_ref}"
     label 'process_single'
 
-    container "docker.io/gallvp/python3npkgs:v0.6"
+    container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
     tuple val(target_on_ref), path(bundle_links)
diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf
index 820f106e..afdd15a2 100644
--- a/modules/local/createreport.nf
+++ b/modules/local/createreport.nf
@@ -2,7 +2,7 @@ process CREATEREPORT {
     tag "AssemblyQC"
     label 'process_single'
 
-    container "docker.io/gallvp/python3npkgs:v0.6"
+    container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
     path fastavalidator_logs, stageAs: 'fastavalidator_logs/*'
diff --git a/modules/local/dnadiff.nf b/modules/local/dnadiff.nf
index 772244e6..71bb93d8 100644
--- a/modules/local/dnadiff.nf
+++ b/modules/local/dnadiff.nf
@@ -9,8 +9,9 @@ process DNADIFF {
     val many_to_many_align
 
     output:
-    tuple val(target_on_ref), path("*.xcoords"), path("*.report")   , emit: coords
-    path "versions.yml"                                             , emit: versions
+    tuple val(target_on_ref), path("*.xcoords") , emit: coords
+    tuple val(target_on_ref), path("*.report")  , emit: report
+    path "versions.yml"                         , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/local/hic2html.nf b/modules/local/hic2html.nf
index 0b907cde..d45e19b3 100644
--- a/modules/local/hic2html.nf
+++ b/modules/local/hic2html.nf
@@ -2,7 +2,7 @@ process HIC2HTML {
     tag "$sample_id_on_tag"
     label 'process_single'
 
-    container "docker.io/gallvp/python3npkgs:v0.6"
+    container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
     tuple val(sample_id_on_tag), path(hic_file)
diff --git a/modules/local/relabelbundlelinks.nf b/modules/local/relabelbundlelinks.nf
index 0b95fb05..ca3431ed 100644
--- a/modules/local/relabelbundlelinks.nf
+++ b/modules/local/relabelbundlelinks.nf
@@ -2,7 +2,7 @@ process RELABELBUNDLELINKS {
     tag "${target_on_ref}"
     label 'process_single'
 
-    container "docker.io/gallvp/python3npkgs:v0.6"
+    container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
     tuple val(target_on_ref), path(coloured_bundle_links), path(target_seq_list), path(ref_seq_list)
diff --git a/modules/local/relabelfastalength.nf b/modules/local/relabelfastalength.nf
index 6c9615ab..b376b5de 100644
--- a/modules/local/relabelfastalength.nf
+++ b/modules/local/relabelfastalength.nf
@@ -2,7 +2,7 @@ process RELABELFASTALENGTH {
     tag "${target_on_ref}"
     label 'process_single'
 
-    container "docker.io/gallvp/python3npkgs:v0.6"
+    container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
     tuple val(target_on_ref), path(target_seq_lengths), path(ref_seq_lengths), path(target_seq_list), path(ref_seq_list)
diff --git a/subworkflows/local/fasta_synteny.nf b/subworkflows/local/fasta_synteny.nf
index 219b3da7..bec3f77b 100644
--- a/subworkflows/local/fasta_synteny.nf
+++ b/subworkflows/local/fasta_synteny.nf
@@ -3,7 +3,7 @@ include { FILTERSORTFASTA               } from '../../modules/local/filtersortfa
 include { MUMMER                        } from '../../modules/local/mummer'
 include { GETFASTALENGTH                } from '../../modules/local/getfastalength'
 include { DNADIFF                       } from '../../modules/local/dnadiff'
-include { CIRCOS_BUNDLELINKS            } from '../../modules/local/circos_bundlelinks'
+include { BUNDLELINKS                   } from '../../modules/local/bundlelinks'
 include { COLOURBUNDLELINKS             } from '../../modules/local/colourbundlelinks'
 include { RELABELBUNDLELINKS            } from '../../modules/local/relabelbundlelinks'
 include { SPLITBUNDLEFILE               } from '../../modules/local/splitbundlefile'
@@ -109,18 +109,18 @@ workflow FASTA_SYNTENY {
 
     ch_versions                         = ch_versions.mix(DNADIFF.out.versions.first())
 
-    // MODULE: CIRCOS_BUNDLELINKS
-    CIRCOS_BUNDLELINKS(
+    // MODULE: BUNDLELINKS
+    BUNDLELINKS(
         DNADIFF.out.coords,
         max_gap,
         min_bundle_size
     )
 
-    ch_versions                         = ch_versions.mix(CIRCOS_BUNDLELINKS.out.versions.first())
+    ch_versions                         = ch_versions.mix(BUNDLELINKS.out.versions.first())
 
     // MODULE: COLOURBUNDLELINKS
     COLOURBUNDLELINKS(
-        CIRCOS_BUNDLELINKS.out.links,
+        BUNDLELINKS.out.links,
         color_by_contig
     )
 

From 5660e89a0aca6d174f1f4a93553e1a4c9f1803ce Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 2 May 2024 12:50:06 +1200
Subject: [PATCH 23/76] Added the ability to create linear synteny plot in
 addition to the circos plot

---
 CHANGELOG.md                                  |   2 +
 bin/assemblyqc.py                             |   4 +-
 bin/linearsynteny.py                          | 199 ++++++++++++++++++
 bin/report_modules/parsers/circos_parser.py   |  52 -----
 bin/report_modules/parsers/synteny_parser.py  | 108 ++++++++++
 bin/report_modules/templates/base.html        |  12 +-
 .../templates/circos/dropdown.html            |  10 -
 .../templates/synteny_circos/dropdown.html    |  10 +
 .../report_contents.html                      |  12 +-
 .../synteny_circos.html}                      |   4 +-
 .../templates/synteny_linear/dropdown.html    |  10 +
 .../synteny_linear/report_contents.html       |  25 +++
 .../synteny_linear/synteny_linear.html        |  30 +++
 conf/base.config                              |   3 +
 docs/parameters.md                            | 141 +++++++------
 modules/local/circos.nf                       |   2 +-
 modules/local/createreport.nf                 |   2 +-
 modules/local/generatekaryotype.nf            |  20 +-
 modules/local/linearsynteny.nf                |  51 +++++
 nextflow.config                               |   3 +-
 nextflow_schema.json                          |  63 ++----
 subworkflows/local/fasta_synteny.nf           |  33 ++-
 workflows/assemblyqc.nf                       |   7 +-
 23 files changed, 600 insertions(+), 203 deletions(-)
 create mode 100755 bin/linearsynteny.py
 delete mode 100644 bin/report_modules/parsers/circos_parser.py
 create mode 100644 bin/report_modules/parsers/synteny_parser.py
 delete mode 100644 bin/report_modules/templates/circos/dropdown.html
 create mode 100644 bin/report_modules/templates/synteny_circos/dropdown.html
 rename bin/report_modules/templates/{circos => synteny_circos}/report_contents.html (60%)
 rename bin/report_modules/templates/{circos/circos.html => synteny_circos/synteny_circos.html} (92%)
 create mode 100644 bin/report_modules/templates/synteny_linear/dropdown.html
 create mode 100644 bin/report_modules/templates/synteny_linear/report_contents.html
 create mode 100644 bin/report_modules/templates/synteny_linear/synteny_linear.html
 create mode 100644 modules/local/linearsynteny.nf

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a47c6aa6..e5f2036b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 4. Added a list of nf-core contributors
 5. Added a launcher script for local testing `local_assemblyqc`
 6. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links
+7. Added the ability to create linear synteny plot in addition to the circos plot
 
 ### `Fixed`
 
@@ -31,6 +32,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### `Deprecated`
 
 1. Removed `CIRCOS_BUNDLELINKS` module
+2. Now the default value of `synteny_plot_1_vs_all` is false
 
 ## 1.4 - [04-Mar-2024]
 
diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py
index fc42eeed..0a2e0b8c 100755
--- a/bin/assemblyqc.py
+++ b/bin/assemblyqc.py
@@ -24,7 +24,7 @@
 from report_modules.parsers.lai_parser import parse_lai_folder
 from report_modules.parsers.kraken2_parser import parse_kraken2_folder
 from report_modules.parsers.hic_parser import parse_hic_folder
-from report_modules.parsers.circos_parser import parse_circos_folder
+from report_modules.parsers.synteny_parser import parse_synteny_folder
 
 if __name__ == "__main__":
     params_dict, params_table = parse_params_json("params_json.json")
@@ -46,7 +46,7 @@
     data_from_tools = {**data_from_tools, **parse_lai_folder()}
     data_from_tools = {**data_from_tools, **parse_kraken2_folder()}
     data_from_tools = {**data_from_tools, **parse_hic_folder()}
-    data_from_tools = {**data_from_tools, **parse_circos_folder()}
+    data_from_tools = {**data_from_tools, **parse_synteny_folder()}
 
     with open("software_versions.yml", "r") as f:
         versions_from_ch_versions = yaml.safe_load(f)
diff --git a/bin/linearsynteny.py b/bin/linearsynteny.py
new file mode 100755
index 00000000..1bc2cd46
--- /dev/null
+++ b/bin/linearsynteny.py
@@ -0,0 +1,199 @@
+#!/usr/bin/env python3
+
+import plotly.graph_objects as go
+import pandas as pd
+import argparse
+
+
+def load_data(data_filename, karyotype_ref_file_name, karyotype_target_filename):
+    data = pd.read_csv(data_filename, sep="\t", header=None)
+    data.columns = [
+        "ref",
+        "ref_start",
+        "ref_stop",
+        "target",
+        "target_start",
+        "target_stop",
+        "color",
+    ]
+
+    karyotype_ref = pd.read_csv(karyotype_ref_file_name, sep="\t", header=None)
+    karyotype_ref.columns = ["chr", "name", "name1", "zero", "size", "color"]
+    karyotype_target = pd.read_csv(karyotype_target_filename, sep="\t", header=None)
+    karyotype_target.columns = ["chr", "name", "name1", "zero", "size", "color"]
+    return data, karyotype_ref, karyotype_target
+
+
+def insert_offsets_and_return_dict(karyotype_df):
+    karyotype_df["offset"] = karyotype_df["size"].cumsum().shift(fill_value=0)
+    return dict(zip(karyotype_df["name"], karyotype_df["offset"]))
+
+
+def insert_midpoints_and_return_dict(karyotype_df):
+    karyotype_df["midpoint"] = (karyotype_df["size"] / 2.0) + karyotype_df["offset"]
+    return dict(zip(karyotype_df["name"], karyotype_df["midpoint"]))
+
+
+def insert_figure_data(
+    data, offsets_ref, offsets_target, midpoints_ref, midpoints_target, fig
+):
+    for index, row in data.iterrows():
+        x_ = [i + offsets_ref[row["ref"]] for i in [row["ref_start"], row["ref_stop"]]]
+
+        y_ = [
+            j + offsets_target[row["target"]]
+            for j in [row["target_start"], row["target_stop"]]
+        ]
+
+        midpoint_x = midpoints_ref[row["ref"]]
+        midpoint_y = midpoints_target[row["target"]]
+
+        fig.add_trace(
+            go.Scatter(
+                x=x_,
+                y=y_,
+                mode="lines",
+                line=dict(
+                    color=f"rgba{(row['color'].replace('color=', '').replace('0.5)', '1)'))}",
+                    width=2,
+                ),
+                name=f"{index}: {row['target']}:{row['ref']}",
+                legendgroup=f"{row['target']}:{row['ref']}",
+                legendgrouptitle=dict(text=f"{row['target']}:{row['ref']}"),
+            )
+        )
+
+        fig.add_trace(
+            go.Scatter(
+                x=[midpoint_x],
+                y=[0],
+                xaxis="x2",
+                line=dict(color="#ffffff"),
+                showlegend=False,
+            )
+        )
+        fig.add_trace(
+            go.Scatter(
+                x=[0],
+                y=[midpoint_y],
+                yaxis="y2",
+                line=dict(color="#ffffff"),
+                showlegend=False,
+            )
+        )
+
+
+def format_figure(
+    karyotype_ref,
+    karyotype_target,
+    offsets_ref,
+    offsets_target,
+    midpoints_ref,
+    midpoints_target,
+    fig,
+):
+
+    xaxis_range = [0, list(offsets_ref.values())[-1] + list(karyotype_ref["size"])[-1]]
+    yaxis_range = [
+        0,
+        list(offsets_target.values())[-1] + list(karyotype_target["size"])[-1],
+    ]
+
+    fig.update_layout(
+        xaxis=dict(
+            range=xaxis_range,
+        ),
+        yaxis=dict(range=yaxis_range),
+        hovermode="closest",
+        xaxis2=dict(
+            overlaying="x",
+            scaleanchor="x1",
+            range=xaxis_range,
+            tickmode="array",
+            tickvals=list(offsets_ref.values()),
+            ticktext=list(offsets_ref.keys()),
+            side="top",
+        ),
+        yaxis2=dict(
+            overlaying="y",
+            scaleanchor="y1",
+            range=yaxis_range,
+            tickmode="array",
+            tickvals=list(offsets_target.values()),
+            ticktext=list(offsets_target.keys()),
+            autoshift=True,
+            anchor="free",
+        ),
+        paper_bgcolor="rgba(0,0,0,0)",
+        plot_bgcolor="rgba(0,0,0,0)",
+        showlegend=True,
+    )
+
+    fig.update_xaxes(
+        showgrid=True,
+        zeroline=False,
+        gridcolor="rgba(0, 0, 0, 0.1)",
+        griddash="dashdot",
+    )
+    fig.update_yaxes(
+        showgrid=True,
+        zeroline=False,
+        gridcolor="rgba(0, 0, 0, 0.1)",
+        griddash="dashdot",
+    )
+    fig["layout"]["yaxis1"]["showgrid"] = False
+    fig["layout"]["xaxis1"]["showgrid"] = False
+
+
+if __name__ == "__main__":
+
+    parser = argparse.ArgumentParser(
+        description="Create a linear synteny plot from `nucmer/dnadiff/circos bundlelinks` bundles",
+        epilog="Author: Usman Rashid",
+    )
+    parser.add_argument("-v", "--version", action="version", version="%(prog)s 0.1")
+    parser.add_argument(
+        "--output",
+        type=str,
+        default="synteny_plot.html",
+        required=False,
+        help="Output filename",
+    )
+    parser.add_argument("bundlelinks", type=argparse.FileType("r"))
+    parser.add_argument("karyotype_ref", type=argparse.FileType("r"))
+    parser.add_argument("karyotype_target", type=argparse.FileType("r"))
+
+    args = parser.parse_args()
+
+    data_filename = args.bundlelinks
+    karyotype_ref_file_name = args.karyotype_ref
+    karyotype_target_filename = args.karyotype_target
+    output_filename = args.output
+
+    data, karyotype_ref, karyotype_target = load_data(
+        data_filename, karyotype_ref_file_name, karyotype_target_filename
+    )
+
+    offsets_ref = insert_offsets_and_return_dict(karyotype_ref)
+    offsets_target = insert_offsets_and_return_dict(karyotype_target)
+
+    midpoints_ref = insert_midpoints_and_return_dict(karyotype_ref)
+    midpoints_target = insert_midpoints_and_return_dict(karyotype_target)
+
+    fig = go.Figure()
+
+    insert_figure_data(
+        data, offsets_ref, offsets_target, midpoints_ref, midpoints_target, fig
+    )
+
+    format_figure(
+        karyotype_ref,
+        karyotype_target,
+        offsets_ref,
+        offsets_target,
+        midpoints_ref,
+        midpoints_target,
+        fig,
+    )
+
+    fig.write_html(output_filename)
diff --git a/bin/report_modules/parsers/circos_parser.py b/bin/report_modules/parsers/circos_parser.py
deleted file mode 100644
index 0df2d037..00000000
--- a/bin/report_modules/parsers/circos_parser.py
+++ /dev/null
@@ -1,52 +0,0 @@
-import os
-from pathlib import Path
-import base64
-import re
-
-from report_modules.parsers.parsing_commons import sort_list_of_results
-
-
-def parse_circos_folder(folder_name="circos_outputs"):
-    dir = os.getcwdb().decode()
-    circos_folder_path = Path(f"{dir}/{folder_name}")
-
-    if not os.path.exists(circos_folder_path):
-        return {}
-
-    list_of_plot_files = [item for item in circos_folder_path.glob("*.png")]
-
-    data = {"CIRCOS": []}
-
-    for plot_path in list_of_plot_files:
-        file_tokens = re.findall(
-            r"([\w]+).on.([\w]+).([\w]+).png",
-            os.path.basename(str(plot_path)),
-        )[0]
-
-        if os.path.getsize(plot_path) == 0:
-            data["CIRCOS"].append(
-                {
-                    "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
-                    "circos_plot": "",
-                    "is_plot_empty": True,
-                }
-            )
-            continue
-
-        binary_fc = open(plot_path, "rb").read()
-        base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8")
-        ext = str(plot_path).split(".")[-1]
-        plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}"
-
-        data["CIRCOS"].append(
-            {
-                "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
-                "circos_plot": plot_url,
-                "is_plot_empty": False,
-            }
-        )
-
-    if len(data["CIRCOS"]) < 1:
-        return {}
-
-    return {"CIRCOS": sort_list_of_results(data["CIRCOS"], "tag.on.tag")}
diff --git a/bin/report_modules/parsers/synteny_parser.py b/bin/report_modules/parsers/synteny_parser.py
new file mode 100644
index 00000000..0f871114
--- /dev/null
+++ b/bin/report_modules/parsers/synteny_parser.py
@@ -0,0 +1,108 @@
+import os
+from pathlib import Path
+import base64
+import re
+
+from report_modules.parsers.parsing_commons import sort_list_of_results
+
+
+def parse_synteny_circos(folder_name="synteny_outputs"):
+    dir = os.getcwdb().decode()
+    circos_folder_path = Path(f"{dir}/{folder_name}")
+
+    if not os.path.exists(circos_folder_path):
+        return {}
+
+    list_of_plot_files = [item for item in circos_folder_path.glob("*.png")]
+
+    data = {"SYNTENY_CIRCOS": []}
+
+    for plot_path in list_of_plot_files:
+        file_tokens = re.findall(
+            r"([\w]+).on.([\w]+).([\w]+).png",
+            os.path.basename(str(plot_path)),
+        )[0]
+
+        if os.path.getsize(plot_path) == 0:
+            data["SYNTENY_CIRCOS"].append(
+                {
+                    "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
+                    "circos_plot": "",
+                    "is_plot_empty": True,
+                }
+            )
+            continue
+
+        binary_fc = open(plot_path, "rb").read()
+        base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8")
+        ext = str(plot_path).split(".")[-1]
+        plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}"
+
+        data["SYNTENY_CIRCOS"].append(
+            {
+                "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
+                "circos_plot": plot_url,
+                "is_plot_empty": False,
+            }
+        )
+
+    if len(data["SYNTENY_CIRCOS"]) < 1:
+        return {}
+
+    return {
+        "SYNTENY_CIRCOS": sort_list_of_results(data["SYNTENY_CIRCOS"], "tag.on.tag")
+    }
+
+
+def parse_synteny_linear(folder_name="synteny_outputs"):
+    dir = os.getcwdb().decode()
+    circos_folder_path = Path(f"{dir}/{folder_name}")
+
+    if not os.path.exists(circos_folder_path):
+        return {}
+
+    list_of_plot_files = [item for item in circos_folder_path.glob("*.html")]
+
+    data = {"SYNTENY_LINEAR": []}
+
+    for plot_path in list_of_plot_files:
+        file_tokens = re.findall(
+            r"([\w]+).on.([\w]+).([\w]+).html",
+            os.path.basename(str(plot_path)),
+        )[0]
+
+        if os.path.getsize(plot_path) == 0:
+            data["SYNTENY_LINEAR"].append(
+                {
+                    "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
+                    "plot": "",
+                    "plot_folder": "",
+                    "is_plot_empty": True,
+                }
+            )
+            continue
+
+        plot_filename = os.path.basename(str(plot_path))
+
+        data["SYNTENY_LINEAR"].append(
+            {
+                "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
+                "plot": plot_filename,
+                "plot_folder": plot_filename.replace(".html", ""),
+                "is_plot_empty": False,
+            }
+        )
+
+    if len(data["SYNTENY_LINEAR"]) < 1:
+        return {}
+
+    return {
+        "SYNTENY_LINEAR": sort_list_of_results(data["SYNTENY_LINEAR"], "tag.on.tag")
+    }
+
+
+def parse_synteny_folder(folder_name="synteny_outputs"):
+    circos_data = parse_synteny_circos(folder_name)
+    linear_data = parse_synteny_linear(folder_name)
+
+    return {**circos_data, **linear_data}
diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html
index bb753a67..2fbb47ce 100644
--- a/bin/report_modules/templates/base.html
+++ b/bin/report_modules/templates/base.html
@@ -33,9 +33,11 @@
       onclick="openTool(event, 'KRAKEN2')">
       KRAKEN2</button>{% endif %} {% if 'HIC' in all_stats_dicts %}<button class="tablinks"
       onclick="openTool(event, 'HIC')">
-      HIC</button>{% endif %} {% if 'CIRCOS' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'CIRCOS')">
-      CIRCOS</button>{% endif %}
+      HIC</button>{% endif %} {% if 'SYNTENY_CIRCOS' in all_stats_dicts %}<button class="tablinks"
+      onclick="openTool(event, 'SYNTENY_CIRCOS')">
+      SYNTENY CIRCOS</button>{% endif %}{% if 'SYNTENY_LINEAR' in all_stats_dicts %}<button class="tablinks"
+      onclick="openTool(event, 'SYNTENY_LINEAR')">
+      SYNTENY LINEAR</button>{% endif %}
   </div>
   {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{%
   include 'fasta_validate/fasta_validate.html' %}{% endif %}
@@ -50,7 +52,9 @@
   include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{%
   endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in
   all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include
-  'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' %}{% endif %}
+  'hic/hic.html' %}{% endif %} {% if 'SYNTENY_CIRCOS' in all_stats_dicts %}{% include
+  'synteny_circos/synteny_circos.html' %}{% endif %} {% if 'SYNTENY_LINEAR' in all_stats_dicts %}{% include
+  'synteny_linear/synteny_linear.html' %}{% endif %}
 </body>
 {% include 'js.html' %}
 
diff --git a/bin/report_modules/templates/circos/dropdown.html b/bin/report_modules/templates/circos/dropdown.html
deleted file mode 100644
index 0cdafbb7..00000000
--- a/bin/report_modules/templates/circos/dropdown.html
+++ /dev/null
@@ -1,10 +0,0 @@
-<div class="dropdown">
-    <div class="dropdown_content">
-        <select id="selector_CIRCOS" onchange="showContent('CIRCOS')">
-            {% set str_tag_on_tag = 'tag.on.tag' %} {% for item in range(all_stats_dicts["CIRCOS"]|length) %}
-            <option value="tabcontent_CIRCOS_{{all_stats_dicts['CIRCOS'][item]['tag.on.tag']}}">
-                {{ all_stats_dicts['CIRCOS'][item][str_tag_on_tag] }} {% endfor %}
-            </option>
-        </select>
-    </div>
-</div>
diff --git a/bin/report_modules/templates/synteny_circos/dropdown.html b/bin/report_modules/templates/synteny_circos/dropdown.html
new file mode 100644
index 00000000..9111391c
--- /dev/null
+++ b/bin/report_modules/templates/synteny_circos/dropdown.html
@@ -0,0 +1,10 @@
+<div class="dropdown">
+    <div class="dropdown_content">
+        <select id="selector_SYNTENY_CIRCOS" onchange="showContent('SYNTENY_CIRCOS')">
+            {% set str_tag_on_tag = 'tag.on.tag' %} {% for item in range(all_stats_dicts["SYNTENY_CIRCOS"]|length) %}
+            <option value="tabcontent_SYNTENY_CIRCOS_{{all_stats_dicts['SYNTENY_CIRCOS'][item]['tag.on.tag']}}">
+                {{ all_stats_dicts['SYNTENY_CIRCOS'][item][str_tag_on_tag] }} {% endfor %}
+            </option>
+        </select>
+    </div>
+</div>
diff --git a/bin/report_modules/templates/circos/report_contents.html b/bin/report_modules/templates/synteny_circos/report_contents.html
similarity index 60%
rename from bin/report_modules/templates/circos/report_contents.html
rename to bin/report_modules/templates/synteny_circos/report_contents.html
index e3355169..e1d975ef 100644
--- a/bin/report_modules/templates/circos/report_contents.html
+++ b/bin/report_modules/templates/synteny_circos/report_contents.html
@@ -1,15 +1,15 @@
-{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["CIRCOS"]|length) %} {% set active_text =
+{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["SYNTENY_CIRCOS"]|length) %} {% set active_text =
 'display: block' if vars.is_first else 'display: none' %}
 <div
-    id="tabcontent_CIRCOS_{{ all_stats_dicts['CIRCOS'][item]['tag.on.tag'] }}"
-    class="tabcontent-CIRCOS"
+    id="tabcontent_SYNTENY_CIRCOS_{{ all_stats_dicts['SYNTENY_CIRCOS'][item]['tag.on.tag'] }}"
+    class="tabcontent-SYNTENY_CIRCOS"
     style="{{ active_text }}"
 >
     <div class="results-section">
         <div class="section-heading-wrapper">
-            <div class="section-heading">{{ all_stats_dicts['CIRCOS'][item]['tag.on.tag'] }}</div>
+            <div class="section-heading">{{ all_stats_dicts['SYNTENY_CIRCOS'][item]['tag.on.tag'] }}</div>
         </div>
-        {% if all_stats_dicts['CIRCOS'][item]['is_plot_empty'] %}
+        {% if all_stats_dicts['SYNTENY_CIRCOS'][item]['is_plot_empty'] %}
         <div class="section-para-wrapper">
             <p class="section-para">
                 Either no alignments were detected between the target and reference assemblies, or all the alignment
@@ -18,7 +18,7 @@
         </div>
         {% else %}
         <div class="image-wrapper">
-            <img src="{{ all_stats_dicts['CIRCOS'][item]['circos_plot'] }}" alt="" />
+            <img src="{{ all_stats_dicts['SYNTENY_CIRCOS'][item]['circos_plot'] }}" alt="" />
         </div>
         {% endif %}
     </div>
diff --git a/bin/report_modules/templates/circos/circos.html b/bin/report_modules/templates/synteny_circos/synteny_circos.html
similarity index 92%
rename from bin/report_modules/templates/circos/circos.html
rename to bin/report_modules/templates/synteny_circos/synteny_circos.html
index 4b9c8c1b..2950621a 100644
--- a/bin/report_modules/templates/circos/circos.html
+++ b/bin/report_modules/templates/synteny_circos/synteny_circos.html
@@ -1,4 +1,4 @@
-<div id="CIRCOS" class="tabcontent" style="display: none">
+<div id="SYNTENY_CIRCOS" class="tabcontent" style="display: none">
     <div class="section-para-wrapper">
         <p class="section-para">
             Circos facilitates the identification and analysis of similarities and differences arising from comparisons
@@ -35,5 +35,5 @@
           </li>
         </ul>
     </div>
-    {% include 'circos/dropdown.html' %} {% include 'circos/report_contents.html' %}
+    {% include 'synteny_circos/dropdown.html' %} {% include 'synteny_circos/report_contents.html' %}
 </div>
diff --git a/bin/report_modules/templates/synteny_linear/dropdown.html b/bin/report_modules/templates/synteny_linear/dropdown.html
new file mode 100644
index 00000000..cfdf3956
--- /dev/null
+++ b/bin/report_modules/templates/synteny_linear/dropdown.html
@@ -0,0 +1,10 @@
+<div class="dropdown">
+  <div class="dropdown_content">
+      <select id="selector_SYNTENY_LINEAR" onchange="showContent('SYNTENY_LINEAR')">
+          {% set str_tag_on_tag = 'tag.on.tag' %} {% for item in range(all_stats_dicts["SYNTENY_LINEAR"]|length) %}
+          <option value="tabcontent_SYNTENY_LINEAR_{{all_stats_dicts['SYNTENY_LINEAR'][item]['tag.on.tag']}}">
+              {{ all_stats_dicts['SYNTENY_LINEAR'][item][str_tag_on_tag] }} {% endfor %}
+          </option>
+      </select>
+  </div>
+</div>
diff --git a/bin/report_modules/templates/synteny_linear/report_contents.html b/bin/report_modules/templates/synteny_linear/report_contents.html
new file mode 100644
index 00000000..069fa018
--- /dev/null
+++ b/bin/report_modules/templates/synteny_linear/report_contents.html
@@ -0,0 +1,25 @@
+{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["SYNTENY_LINEAR"]|length) %} {% set active_text
+= 'display: block' if vars.is_first else 'display: none' %}
+<div id="tabcontent_SYNTENY_LINEAR_{{ all_stats_dicts['SYNTENY_LINEAR'][item]['tag.on.tag'] }}"
+  class="tabcontent-SYNTENY_LINEAR" style="{{ active_text }}">
+  <div class="results-section">
+    <div class="section-heading-wrapper">
+      <div class="section-heading">{{ all_stats_dicts['SYNTENY_LINEAR'][item]['tag.on.tag'] }}</div>
+    </div>
+    {% if all_stats_dicts['SYNTENY_LINEAR'][item]['is_plot_empty'] %}
+    <div class="section-para-wrapper">
+      <p class="section-para">
+        Either no alignments were detected between the target and reference assemblies, or all the alignment
+        bundles were filtered out 😔
+      </p>
+    </div>
+    {% else %}
+    <div class="iframe-wrapper">
+      <iframe
+        src="./synteny/{{ all_stats_dicts['SYNTENY_LINEAR'][item]['plot_folder'] }}/{{ all_stats_dicts['SYNTENY_LINEAR'][item]['plot'] }}"
+        width="100%" height="100%"></iframe>
+    </div>
+    {% endif %}
+  </div>
+</div>
+{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %}
diff --git a/bin/report_modules/templates/synteny_linear/synteny_linear.html b/bin/report_modules/templates/synteny_linear/synteny_linear.html
new file mode 100644
index 00000000..6be686e6
--- /dev/null
+++ b/bin/report_modules/templates/synteny_linear/synteny_linear.html
@@ -0,0 +1,30 @@
+<div id="SYNTENY_LINEAR" class="tabcontent" style="display: none">
+  <div class="section-para-wrapper">
+    <p class="section-para">The genome-wide alignments are performed with MUMMER.</p>
+    <p class="section-para"><b>References:</b></p>
+    <p class="section-para">
+      Krzywinski, M., Schein, J., Birol, I., Connors, J., Gascoyne, R., Horsman, D., ... & Marra, M. A. (2009).
+      Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645.
+      <a href="https://doi.org/10.1101/gr.092759.109" target="_blank">https://doi.org/10.1101/gr.092759.109</a>
+    </p>
+    <p class="section-para">
+      <b>Version: {{ all_stats_dicts['VERSIONS']['MUMMER']['nucmer'] }} (MUMMER)</b>
+    </p>
+    <p class="section-para"><b>Notes:</b></p>
+    <ul>
+      <li>
+        Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny_max_gap'] }}bp have been
+        bundled together.
+      </li>
+      <li>
+        After bundling, any bundle smaller than {{ all_stats_dicts['PARAMS_DICT']['synteny_min_bundle_size'] }}bp has
+        been filtered out.
+      </li>
+      <li>
+        The sequence labels shown on the plot are based on the labelling file provided to the pipeline. These labels may
+        or may not be same as the sequence IDs in the corresponding FASTA files.
+      </li>
+    </ul>
+  </div>
+  {% include 'synteny_linear/dropdown.html' %} {% include 'synteny_linear/report_contents.html' %}
+</div>
diff --git a/conf/base.config b/conf/base.config
index 0e066d52..1f1878db 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -72,4 +72,7 @@ process {
     withName:DNADIFF {
         time   = { check_max( 7.day  * task.attempt, 'time'    ) }
     }
+    withName:CREATEREPORT {
+        cache   = false
+    }
 }
diff --git a/docs/parameters.md b/docs/parameters.md
index f02e854a..dd749c5b 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -4,45 +4,45 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## Input/output options
 
-| Parameter | Description                                                                                                                                                                                                                                                                                                                                                 | Type     | Default   | Required | Hidden |
-| --------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | --------- | -------- | ------ |
-| `input`   | Input assembly sheet in CSV format <details><summary>Help</summary><small>FASTA and other associated files for input assemblies provided as a formatted CSV file</small></details>                                                                                                                                                                          | `string` |           | True     |        |
-| `outdir`  | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.                                                                                                                                                                                                                                    | `string` | ./results | True     |        |
-| `email`   | Email address for completion summary. <details><summary>Help</summary><small>Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.</small></details> | `string` |           |          |        |
+| Parameter | Description                                                                                                              | Type     | Default   | Required | Hidden |
+| --------- | ------------------------------------------------------------------------------------------------------------------------ | -------- | --------- | -------- | ------ |
+| `input`   | Input assembly sheet in CSV format                                                                                       | `string` |           | True     |        |
+| `outdir`  | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure. | `string` | ./results | True     |        |
+| `email`   | Email address for completion summary.                                                                                    | `string` |           |          |        |
 
 ## General stats options
 
-| Parameter                    | Description                                                                                                                                                                                                                                                                                                          | Type      | Default | Required | Hidden |
-| ---------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size <details><summary>Help</summary><small>This number is used to split the scaffolds into contigs to compute contig-related stats such as the number of contigs, N50, etc. NCBI recommendation <https://www.ncbi.nlm.nih.gov/genbank/wgs_gapped/> is 100.</small></details> | `integer` | 100     |          |        |
+| Parameter                    | Description                                                             | Type      | Default | Required | Hidden |
+| ---------------------------- | ----------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size. NCBI recommendation is 100 | `integer` | 100     |          |        |
 
 ## NCBI FCS options
 
-| Parameter                 | Description                                                                                                                                                                                                                                                                                                                         | Type      | Default | Required | Hidden |
-| ------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `ncbi_fcs_adaptor_skip`   | Skip NCBI FCS Adaptor checking                                                                                                                                                                                                                                                                                                      | `boolean` | True    |          |        |
-| `ncbi_fcs_adaptor_empire` | Empire for NCBI FCS Adaptor checking <details><summary>Help</summary><small>'euk' for eukaryotes, or 'prok' for prokaryotes</small></details>                                                                                                                                                                                       | `string`  |         |          |        |
-| `ncbi_fcs_gx_skip`        | Skip NCBI FCS external organism contamination checking                                                                                                                                                                                                                                                                              | `boolean` | True    |          |        |
-| `ncbi_fcs_gx_tax_id`      | Tax ID for NCBI FCS GX <details><summary>Help</summary><small>Get correct tax ID from https://www.ncbi.nlm.nih.gov/taxonomy</small></details>                                                                                                                                                                                       | `number`  |         |          |        |
-| `ncbi_fcs_gx_db_path`     | Path to NCBI FCS GX database <details><summary>Help</summary><small>NCBI FCS GX DB path<br><br>Due to enormity of the DB size, the pipeline does NOT download the data. It must be setup by the user manually before running the pipeline. See instructions for DB setup: https://github.com/ncbi/fcs/wiki/FCS-GX</small></details> | `string`  |         |          |        |
+| Parameter                 | Description                                                                           | Type      | Default | Required | Hidden |
+| ------------------------- | ------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `ncbi_fcs_adaptor_skip`   | Skip NCBI FCS Adaptor checking                                                        | `boolean` | True    |          |        |
+| `ncbi_fcs_adaptor_empire` | Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes | `string`  |         |          |        |
+| `ncbi_fcs_gx_skip`        | Skip NCBI FCS external organism contamination checking                                | `boolean` | True    |          |        |
+| `ncbi_fcs_gx_tax_id`      | Tax ID for NCBI FCS GX. See: https://www.ncbi.nlm.nih.gov/taxonomy                    | `number`  |         |          |        |
+| `ncbi_fcs_gx_db_path`     | Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX            | `string`  |         |          |        |
 
 ## BUSCO options
 
-| Parameter                | Description                                                                                                                                                                                                                              | Type      | Default | Required | Hidden |
-| ------------------------ | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `busco_skip`             | Skip BUSCO                                                                                                                                                                                                                               | `boolean` | True    |          |        |
-| `busco_mode`             | BUSCO mode <details><summary>Help</summary><small>'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'proteins' for annotated gene sets (protein)</small></details> | `string`  |         |          |        |
-| `busco_lineage_datasets` | BUSCO lineages <details><summary>Help</summary><small>Each input assembly is assessed against each lineage. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' </small></details>            | `string`  |         |          |        |
-| `busco_download_path`    | Download path for BUSCO <details><summary>Help</summary><small>BUSCO DB download path<br><br>The pipeline automatically downloads the required DB if needed</small></details>                                                            | `string`  |         |          |        |
+| Parameter                | Description                                                                                                    | Type      | Default | Required | Hidden |
+| ------------------------ | -------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `busco_skip`             | Skip BUSCO                                                                                                     | `boolean` | True    |          |        |
+| `busco_mode`             | BUSCO mode: 'geno' for genome, 'tran' for transcriptome, 'prot' for proteins                                   | `string`  |         |          |        |
+| `busco_lineage_datasets` | BUSCO lineages. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' | `string`  |         |          |        |
+| `busco_download_path`    | Download path for BUSCO                                                                                        | `string`  |         |          |        |
 
 ## TIDK options
 
-| Parameter             | Description                                                                                                                                  | Type      | Default | Required | Hidden |
-| --------------------- | -------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `tidk_skip`           | Skip telomere identification                                                                                                                 | `boolean` | True    |          |        |
-| `tidk_repeat_seq`     | Telomere repeat sequence <details><summary>Help</summary><small>Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG</small></details> | `string`  |         |          |        |
-| `tidk_filter_by_size` | Filter size in base-pairs                                                                                                                    | `boolean` |         |          |        |
-| `tidk_filter_size_bp` | Filter size in base-pairs                                                                                                                    | `integer` | 1000000 |          |        |
+| Parameter             | Description                                                                          | Type      | Default | Required | Hidden |
+| --------------------- | ------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
+| `tidk_skip`           | Skip telomere identification                                                         | `boolean` | True    |          |        |
+| `tidk_repeat_seq`     | Telomere repeat sequence. Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG | `string`  |         |          |        |
+| `tidk_filter_by_size` | Filter size in base-pairs                                                            | `boolean` |         |          |        |
+| `tidk_filter_size_bp` | Filter size in base-pairs                                                            | `integer` | 1000000 |          |        |
 
 ## LAI options
 
@@ -59,64 +59,65 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## HiC options
 
-| Parameter            | Description                                                                                                                                   | Type                      | Default                                           | Required | Hidden |
-| -------------------- | --------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------- | ------------------------------------------------- | -------- | ------ | --- |
-| `hic`                | HiC reads <details><summary>Help</summary><small>Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq | fq).gz'</small></details> | `string`                                          |          |        |     |
-| `hic_skip_fastp`     | Skip HiC read trimming                                                                                                                        | `boolean`                 |                                                   |          |        |
-| `hic_skip_fastqc`    | Skip HiC read QC                                                                                                                              | `boolean`                 |                                                   |          |        |
-| `hic_fastp_ext_args` | Additional parameters for fastp trimming                                                                                                      | `string`                  | --qualified_quality_phred 20 --length_required 50 |          |        |
+| Parameter            | Description                                                                         | Type      | Default                                           | Required | Hidden |
+| -------------------- | ----------------------------------------------------------------------------------- | --------- | ------------------------------------------------- | -------- | ------ | --- |
+| `hic`                | HiC reads path provided as a SRA ID or as paired reads with pattern '\*{1,2}.(fastq | fq).gz'   | `string`                                          |          |        |     |
+| `hic_skip_fastp`     | Skip HiC read trimming                                                              | `boolean` |                                                   |          |        |
+| `hic_skip_fastqc`    | Skip HiC read QC                                                                    | `boolean` |                                                   |          |        |
+| `hic_fastp_ext_args` | Additional parameters for fastp trimming                                            | `string`  | --qualified_quality_phred 20 --length_required 50 |          |        |
 
 ## Synteny options
 
-| Parameter                          | Description                                                                                                                                                                      | Type      | Default | Required | Hidden |
-| ---------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `synteny_skip`                     | Skip synteny analysis                                                                                                                                                            | `boolean` | True    |          |        |
-| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies                                                                                                                      | `boolean` | True    |          |        |
-| `synteny_many_to_many_align`       | Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)                                                                                                      | `boolean` |         |          |        |
-| `synteny_max_gap`                  | Alignments within this distance are bundled together                                                                                                                             | `integer` | 1000000 |          |        |
-| `synteny_min_bundle_size`          | After bundling, any bundle smaller than this size is filtered out                                                                                                                | `integer` | 1000    |          |        |
-| `synteny_plot_1_vs_all`            | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly                                                               | `boolean` | True    |          |        |
-| `synteny_color_by_contig`          | Synteny plot is colored by contig                                                                                                                                                | `boolean` | True    |          |        |
-| `synteny_xref_assemblies`          | Reference assemblies for synteny analysis <details><summary>Help</summary><small>FASTA and synteny label tsv files should be provided in a formatted CSV file </small></details> | `string`  |         |          |        |
+| Parameter                          | Description                                                                                                        | Type      | Default | Required | Hidden |
+| ---------------------------------- | ------------------------------------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
+| `synteny_skip`                     | Skip synteny analysis                                                                                              | `boolean` | True    |          |        |
+| `synteny_plot_type`                | Synteny plot type: 'linear', 'circos', or 'both'                                                                   | `string`  | both    |          |        |
+| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies                                                        | `boolean` | True    |          |        |
+| `synteny_many_to_many_align`       | Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)                                        | `boolean` |         |          |        |
+| `synteny_max_gap`                  | Alignments within this distance are bundled together                                                               | `integer` | 1000000 |          |        |
+| `synteny_min_bundle_size`          | After bundling, any bundle smaller than this size is filtered out                                                  | `integer` | 1000    |          |        |
+| `synteny_plot_1_vs_all`            | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly | `boolean` |         |          |        |
+| `synteny_color_by_contig`          | Synteny plot is colored by contig                                                                                  | `boolean` | True    |          |        |
+| `synteny_xref_assemblies`          | Reference assemblies for synteny analysis                                                                          | `string`  |         |          |        |
 
 ## Max job request options
 
 Set the top limit for requested resources for any single job.
 
-| Parameter    | Description                                                                                                                                                                                                                                                                 | Type      | Default | Required | Hidden |
-| ------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `max_cpus`   | Maximum number of CPUs that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`</small></details>                                      | `integer` | 16      |          | True   |
-| `max_memory` | Maximum amount of memory that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`</small></details> | `string`  | 512.GB  |          | True   |
-| `max_time`   | Maximum amount of time that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`</small></details>        | `string`  | 7.day   |          | True   |
+| Parameter    | Description                                                                        | Type      | Default | Required | Hidden |
+| ------------ | ---------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `max_cpus`   | Maximum number of CPUs that can be requested for any single job.                   | `integer` | 16      |          | True   |
+| `max_memory` | Maximum amount of memory that can be requested for any single job. Example: '8.GB' | `string`  | 512.GB  |          | True   |
+| `max_time`   | Maximum amount of time that can be requested for any single job. Example: '1.day'  | `string`  | 7.day   |          | True   |
 
 ## Institutional config options
 
 Parameters used to describe centralised config profiles. These should not be edited.
 
-| Parameter                    | Description                                                                                                                                                                                                                                                                                                                                                                                       | Type     | Default                                                  | Required | Hidden |
-| ---------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | -------------------------------------------------------- | -------- | ------ |
-| `custom_config_version`      | Git commit id for Institutional configs.                                                                                                                                                                                                                                                                                                                                                          | `string` | master                                                   |          | True   |
-| `custom_config_base`         | Base directory for Institutional configs. <details><summary>Help</summary><small>If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.</small></details> | `string` | https://raw.githubusercontent.com/nf-core/configs/master |          | True   |
-| `config_profile_name`        | Institutional config name.                                                                                                                                                                                                                                                                                                                                                                        | `string` |                                                          |          | True   |
-| `config_profile_description` | Institutional config description.                                                                                                                                                                                                                                                                                                                                                                 | `string` |                                                          |          | True   |
-| `config_profile_contact`     | Institutional config contact information.                                                                                                                                                                                                                                                                                                                                                         | `string` |                                                          |          | True   |
-| `config_profile_url`         | Institutional config URL link.                                                                                                                                                                                                                                                                                                                                                                    | `string` |                                                          |          | True   |
+| Parameter                    | Description                               | Type     | Default                                                  | Required | Hidden |
+| ---------------------------- | ----------------------------------------- | -------- | -------------------------------------------------------- | -------- | ------ |
+| `custom_config_version`      | Git commit id for Institutional configs.  | `string` | master                                                   |          | True   |
+| `custom_config_base`         | Base directory for Institutional configs. | `string` | https://raw.githubusercontent.com/nf-core/configs/master |          | True   |
+| `config_profile_name`        | Institutional config name.                | `string` |                                                          |          | True   |
+| `config_profile_description` | Institutional config description.         | `string` |                                                          |          | True   |
+| `config_profile_contact`     | Institutional config contact information. | `string` |                                                          |          | True   |
+| `config_profile_url`         | Institutional config URL link.            | `string` |                                                          |          | True   |
 
 ## Generic options
 
 Less common options for the pipeline, typically set in a config file.
 
-| Parameter                          | Description                                                                                                                                                                                                                                                                                                                                                                                                  | Type      | Default | Required | Hidden |
-| ---------------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
-| `help`                             | Display help text.                                                                                                                                                                                                                                                                                                                                                                                           | `boolean` |         |          | True   |
-| `version`                          | Display version and exit.                                                                                                                                                                                                                                                                                                                                                                                    | `boolean` |         |          | True   |
-| `publish_dir_mode`                 | Method used to save pipeline results to output directory. <details><summary>Help</summary><small>The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.</small></details> | `string`  | copy    |          | True   |
-| `email_on_fail`                    | Email address for completion summary, only when pipeline fails. <details><summary>Help</summary><small>An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.</small></details>                                                                                                                                                  | `string`  |         |          | True   |
-| `plaintext_email`                  | Send plain-text email instead of HTML.                                                                                                                                                                                                                                                                                                                                                                       | `boolean` |         |          | True   |
-| `monochrome_logs`                  | Do not use coloured log outputs.                                                                                                                                                                                                                                                                                                                                                                             | `boolean` |         |          | True   |
-| `monochromeLogs`                   | Do not use coloured log outputs.                                                                                                                                                                                                                                                                                                                                                                             | `boolean` |         |          | True   |
-| `hook_url`                         | Incoming hook URL for messaging service <details><summary>Help</summary><small>Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.</small></details>                                                                                                                                                                                                                       | `string`  |         |          | True   |
-| `validate_params`                  | Boolean whether to validate parameters against the schema at runtime                                                                                                                                                                                                                                                                                                                                         | `boolean` | True    |          | True   |
-| `validationShowHiddenParams`       | Show all params when using `--help` <details><summary>Help</summary><small>By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters.</small></details>                                                                                                                    | `boolean` |         |          | True   |
-| `validationFailUnrecognisedParams` | Validation of parameters fails when an unrecognised parameter is found. <details><summary>Help</summary><small>By default, when an unrecognised parameter is found, it returns a warinig.</small></details>                                                                                                                                                                                                  | `boolean` |         |          | True   |
-| `validationLenientMode`            | Validation of parameters in lenient more. <details><summary>Help</summary><small>Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode).</small></details>                                                                                                                                    | `boolean` |         |          | True   |
+| Parameter                          | Description                                                             | Type      | Default | Required | Hidden |
+| ---------------------------------- | ----------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `help`                             | Display help text.                                                      | `boolean` |         |          | True   |
+| `version`                          | Display version and exit.                                               | `boolean` |         |          | True   |
+| `publish_dir_mode`                 | Method used to save pipeline results to output directory.               | `string`  | copy    |          | True   |
+| `email_on_fail`                    | Email address for completion summary, only when pipeline fails.         | `string`  |         |          | True   |
+| `plaintext_email`                  | Send plain-text email instead of HTML.                                  | `boolean` |         |          | True   |
+| `monochrome_logs`                  | Do not use coloured log outputs.                                        | `boolean` |         |          | True   |
+| `monochromeLogs`                   | Do not use coloured log outputs.                                        | `boolean` |         |          | True   |
+| `hook_url`                         | Incoming hook URL for messaging service                                 | `string`  |         |          | True   |
+| `validate_params`                  | Boolean whether to validate parameters against the schema at runtime    | `boolean` | True    |          | True   |
+| `validationShowHiddenParams`       | Show all params when using `--help`                                     | `boolean` |         |          | True   |
+| `validationFailUnrecognisedParams` | Validation of parameters fails when an unrecognised parameter is found. | `boolean` |         |          | True   |
+| `validationLenientMode`            | Validation of parameters in lenient more.                               | `boolean` |         |          | True   |
diff --git a/modules/local/circos.nf b/modules/local/circos.nf
index 8311e694..dd755c46 100644
--- a/modules/local/circos.nf
+++ b/modules/local/circos.nf
@@ -5,7 +5,7 @@ process CIRCOS {
     container "docker.io/gallvp/circos-tools:v0.23-1_ps"
 
     input:
-    tuple val(target_on_ref_seq), path(karyotype), path(bundle_file)
+    tuple val(target_on_ref_seq), path(bundle_file), path(karyotype)
 
     output:
     path "*.svg", emit: svg_file
diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf
index afdd15a2..8deb0edb 100644
--- a/modules/local/createreport.nf
+++ b/modules/local/createreport.nf
@@ -16,7 +16,7 @@ process CREATEREPORT {
     path lai_outputs, stageAs: 'lai_outputs/*'
     path kraken2_outputs, stageAs: 'kraken2_outputs/*'
     path hic_outputs, stageAs: 'hic_outputs/*'
-    path circos_outputs, stageAs: 'circos_outputs/*'
+    path synteny_outputs, stageAs: 'synteny_outputs/*'
     path versions
     val params_json
     val params_summary_json
diff --git a/modules/local/generatekaryotype.nf b/modules/local/generatekaryotype.nf
index 0135b8a1..b5ed48fa 100644
--- a/modules/local/generatekaryotype.nf
+++ b/modules/local/generatekaryotype.nf
@@ -10,8 +10,10 @@ process GENERATEKARYOTYPE {
     tuple val(target_on_ref), val(seq_tag), path(split_bundle_file), path(target_seq_len), path(ref_seq_len)
 
     output:
-    tuple val("${target_on_ref}.${seq_tag}"), path("*.karyotype")   , emit: karyotype
-    path "versions.yml"                                             , emit: versions
+    tuple val("${target_on_ref}.${seq_tag}"), path("*.karyotype")           , emit: karyotype
+    tuple val("${target_on_ref}.${seq_tag}"), path("karyotype_ref.tsv")     , emit: karyotype_ref
+    tuple val("${target_on_ref}.${seq_tag}"), path("karyotype_target.tsv")  , emit: karyotype_target
+    path "versions.yml"                                                     , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -48,12 +50,24 @@ process GENERATEKARYOTYPE {
 
     cat colored.filtered.ref.seq.len | sort -k1V > merged.seq.lengths
     cat colored.filtered.target.seq.len | sort -k1Vr >> merged.seq.lengths
-    sed -i '/^\$/d' merged.seq.lengths
 
     cat merged.seq.lengths \
+    | sed '/^\$/d' \
     | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \
     > "${target_on_ref}.${seq_tag}.karyotype"
 
+    cat colored.filtered.ref.seq.len \
+    | sort -k1V \
+    | sed '/^\$/d' \
+    | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \
+    > karyotype_ref.tsv
+
+    cat colored.filtered.target.seq.len \
+    | sort -k1V \
+    | sed '/^\$/d' \
+    | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \
+    > karyotype_target.tsv
+
     rm "\$tmp_file"
     """
 }
diff --git a/modules/local/linearsynteny.nf b/modules/local/linearsynteny.nf
new file mode 100644
index 00000000..1296a658
--- /dev/null
+++ b/modules/local/linearsynteny.nf
@@ -0,0 +1,51 @@
+process LINEARSYNTENY {
+    tag "${target_on_ref_seq}"
+    label "process_single"
+
+    container "docker.io/gallvp/python3npkgs:v0.7"
+    publishDir "${params.outdir}/synteny/${target_on_ref_seq}", mode: 'copy'
+
+    input:
+    tuple val(target_on_ref_seq), path(bundle_file), path(karyotype_ref), path(karyotype_target)
+
+    output:
+    path "*.html"               , emit: html
+    path "bundled.links.tsv"    , emit: bundled_links_tsv
+    path "versions.yml"         , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    cat $bundle_file \\
+        | awk '{print \$1,\$2,\$3,\$4,\$5,\$6,\$7}' OFS="\\t" \\
+        > bundled.links.tsv
+
+    linearsynteny.py \\
+        bundled.links.tsv \\
+        $karyotype_ref \\
+        $karyotype_target \\
+        --output "${target_on_ref_seq}.html"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | tr -d 'Python[:space:]')
+        pandas: \$(python -c "import pandas; print(pandas.__version__)")
+        plotly: \$(python -c "import plotly; print(plotly.__version__)")
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch bundled.links.tsv
+    touch "${target_on_ref_seq}.html"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | tr -d 'Python[:space:]')
+        pandas: \$(python -c "import pandas; print(pandas.__version__)")
+        plotly: \$(python -c "import plotly; print(plotly.__version__)")
+    END_VERSIONS
+    """
+}
diff --git a/nextflow.config b/nextflow.config
index 84ddfb0c..9aa0e97b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -50,11 +50,12 @@ params {
 
     // Synteny options
     synteny_skip                        = true
+    synteny_plot_type                   = 'both'
     synteny_between_input_assemblies    = true
     synteny_many_to_many_align          = false
     synteny_max_gap                     = 1000000
     synteny_min_bundle_size             = 1000
-    synteny_plot_1_vs_all               = true
+    synteny_plot_1_vs_all               = false
     synteny_color_by_contig             = true
     synteny_xref_assemblies             = null
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 96e5b50d..6e683bfb 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -18,7 +18,6 @@
                     "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",
-                    "help_text": "FASTA and other associated files for input assemblies provided as a formatted CSV file",
                     "description": "Input assembly sheet in CSV format",
                     "fa_icon": "fas fa-file-csv"
                 },
@@ -33,7 +32,6 @@
                     "type": "string",
                     "description": "Email address for completion summary.",
                     "fa_icon": "fas fa-envelope",
-                    "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.",
                     "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$"
                 }
             },
@@ -48,8 +46,7 @@
                 "assemblathon_stats_n_limit": {
                     "type": "integer",
                     "default": 100,
-                    "help_text": "This number is used to split the scaffolds into contigs to compute contig-related stats such as the number of contigs, N50, etc. NCBI recommendation <https://www.ncbi.nlm.nih.gov/genbank/wgs_gapped/> is 100.",
-                    "description": "The number of 'N's for the unknown gap size"
+                    "description": "The number of 'N's for the unknown gap size. NCBI recommendation is 100"
                 }
             }
         },
@@ -67,8 +64,7 @@
                 "ncbi_fcs_adaptor_empire": {
                     "type": "string",
                     "enum": ["euk", "prok"],
-                    "description": "Empire for NCBI FCS Adaptor checking",
-                    "help_text": "'euk' for eukaryotes, or 'prok' for prokaryotes"
+                    "description": "Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes"
                 },
                 "ncbi_fcs_gx_skip": {
                     "type": "boolean",
@@ -77,14 +73,12 @@
                 },
                 "ncbi_fcs_gx_tax_id": {
                     "type": "number",
-                    "help_text": "Get correct tax ID from https://www.ncbi.nlm.nih.gov/taxonomy",
-                    "description": "Tax ID for NCBI FCS GX"
+                    "description": "Tax ID for NCBI FCS GX. See: https://www.ncbi.nlm.nih.gov/taxonomy"
                 },
                 "ncbi_fcs_gx_db_path": {
                     "type": "string",
                     "format": "directory-path",
-                    "help_text": "NCBI FCS GX DB path\n\nDue to enormity of the DB size, the pipeline does NOT download the data. It must be setup by the user manually before running the pipeline. See instructions for DB setup: https://github.com/ncbi/fcs/wiki/FCS-GX",
-                    "description": "Path to NCBI FCS GX database"
+                    "description": "Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX"
                 }
             }
         },
@@ -102,18 +96,15 @@
                 "busco_mode": {
                     "type": "string",
                     "enum": ["geno", "tran", "prot", "genome", "transcriptome", "proteins"],
-                    "help_text": "'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'proteins' for annotated gene sets (protein)",
-                    "description": "BUSCO mode"
+                    "description": "BUSCO mode: 'geno' for genome, 'tran' for transcriptome, 'prot' for proteins"
                 },
                 "busco_lineage_datasets": {
                     "type": "string",
-                    "help_text": "Each input assembly is assessed against each lineage. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' ",
                     "pattern": "^(\\w+_odb10\\s)*\\w+_odb10$",
-                    "description": "BUSCO lineages"
+                    "description": "BUSCO lineages. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10'"
                 },
                 "busco_download_path": {
                     "type": "string",
-                    "help_text": "BUSCO DB download path\n\nThe pipeline automatically downloads the required DB if needed",
                     "description": "Download path for BUSCO",
                     "format": "directory-path"
                 }
@@ -132,9 +123,8 @@
                 },
                 "tidk_repeat_seq": {
                     "type": "string",
-                    "description": "Telomere repeat sequence",
-                    "pattern": "^[ACGT]+$",
-                    "help_text": "Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG"
+                    "description": "Telomere repeat sequence. Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG",
+                    "pattern": "^[ACGT]+$"
                 },
                 "tidk_filter_by_size": {
                     "type": "boolean",
@@ -186,8 +176,7 @@
             "properties": {
                 "hic": {
                     "type": "string",
-                    "description": "HiC reads",
-                    "help_text": "Path to reads provided as a SRA ID or as a path to paired reads with pattern '*{1,2}.(fastq|fq).gz'",
+                    "description": "HiC reads path provided as a SRA ID or as paired reads with pattern '*{1,2}.(fastq|fq).gz'",
                     "pattern": "^SR\\w+$|^\\S+\\{1,2\\}\\.f(ast)?q\\.gz$"
                 },
                 "hic_skip_fastp": {
@@ -216,6 +205,12 @@
                     "default": true,
                     "description": "Skip synteny analysis"
                 },
+                "synteny_plot_type": {
+                    "type": "string",
+                    "default": "both",
+                    "description": "Synteny plot type: 'linear', 'circos', or 'both'",
+                    "enum": ["both", "linear", "circos"]
+                },
                 "synteny_between_input_assemblies": {
                     "type": "boolean",
                     "description": "Create syntenic plots between each pair of input assemblies",
@@ -237,7 +232,6 @@
                 },
                 "synteny_plot_1_vs_all": {
                     "type": "boolean",
-                    "default": true,
                     "description": "Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly"
                 },
                 "synteny_color_by_contig": {
@@ -248,7 +242,6 @@
                 "synteny_xref_assemblies": {
                     "type": "string",
                     "description": "Reference assemblies for synteny analysis",
-                    "help_text": "FASTA and synteny label tsv files should be provided in a formatted CSV file ",
                     "format": "file-path",
                     "mimetype": "csv",
                     "schema": "assets/schema_xref_assemblies.json"
@@ -267,26 +260,23 @@
                     "description": "Maximum number of CPUs that can be requested for any single job.",
                     "default": 16,
                     "fa_icon": "fas fa-microchip",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`"
+                    "hidden": true
                 },
                 "max_memory": {
                     "type": "string",
-                    "description": "Maximum amount of memory that can be requested for any single job.",
+                    "description": "Maximum amount of memory that can be requested for any single job. Example: '8.GB'",
                     "default": "512.GB",
                     "fa_icon": "fas fa-memory",
                     "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
+                    "hidden": true
                 },
                 "max_time": {
                     "type": "string",
-                    "description": "Maximum amount of time that can be requested for any single job.",
+                    "description": "Maximum amount of time that can be requested for any single job. Example: '1.day'",
                     "default": "7.day",
                     "fa_icon": "far fa-clock",
                     "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
+                    "hidden": true
                 }
             }
         },
@@ -309,7 +299,6 @@
                     "description": "Base directory for Institutional configs.",
                     "default": "https://raw.githubusercontent.com/nf-core/configs/master",
                     "hidden": true,
-                    "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.",
                     "fa_icon": "fas fa-users-cog"
                 },
                 "config_profile_name": {
@@ -361,7 +350,6 @@
                     "type": "string",
                     "default": "copy",
                     "description": "Method used to save pipeline results to output directory.",
-                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
                     "fa_icon": "fas fa-copy",
                     "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
                     "hidden": true
@@ -371,7 +359,6 @@
                     "description": "Email address for completion summary, only when pipeline fails.",
                     "fa_icon": "fas fa-exclamation-triangle",
                     "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$",
-                    "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.",
                     "hidden": true
                 },
                 "plaintext_email": {
@@ -396,7 +383,6 @@
                     "type": "string",
                     "description": "Incoming hook URL for messaging service",
                     "fa_icon": "fas fa-people-group",
-                    "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.",
                     "hidden": true
                 },
                 "validate_params": {
@@ -410,22 +396,19 @@
                     "type": "boolean",
                     "fa_icon": "far fa-eye-slash",
                     "description": "Show all params when using `--help`",
-                    "hidden": true,
-                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
+                    "hidden": true
                 },
                 "validationFailUnrecognisedParams": {
                     "type": "boolean",
                     "fa_icon": "far fa-check-circle",
                     "description": "Validation of parameters fails when an unrecognised parameter is found.",
-                    "hidden": true,
-                    "help_text": "By default, when an unrecognised parameter is found, it returns a warinig."
+                    "hidden": true
                 },
                 "validationLenientMode": {
                     "type": "boolean",
                     "fa_icon": "far fa-check-circle",
                     "description": "Validation of parameters in lenient more.",
-                    "hidden": true,
-                    "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
+                    "hidden": true
                 }
             }
         }
diff --git a/subworkflows/local/fasta_synteny.nf b/subworkflows/local/fasta_synteny.nf
index bec3f77b..26d31a0d 100644
--- a/subworkflows/local/fasta_synteny.nf
+++ b/subworkflows/local/fasta_synteny.nf
@@ -10,6 +10,7 @@ include { SPLITBUNDLEFILE               } from '../../modules/local/splitbundlef
 include { RELABELFASTALENGTH            } from '../../modules/local/relabelfastalength'
 include { GENERATEKARYOTYPE             } from '../../modules/local/generatekaryotype'
 include { CIRCOS                        } from '../../modules/local/circos'
+include { LINEARSYNTENY                 } from '../../modules/local/linearsynteny'
 
 workflow FASTA_SYNTENY {
     take:
@@ -22,6 +23,7 @@ workflow FASTA_SYNTENY {
     min_bundle_size                     // val(Integer)
     plot_1_vs_all                       // val(true|false)
     color_by_contig                     // val(true|false)
+    plot_type                           // val(linear|circos|both)
 
     main:
     ch_versions                         = Channel.empty()
@@ -176,19 +178,34 @@ workflow FASTA_SYNTENY {
     ch_versions                         = ch_versions.mix(GENERATEKARYOTYPE.out.versions.first())
 
     // MODULE: CIRCOS
-    ch_circos_inputs                    = GENERATEKARYOTYPE.out.karyotype
-                                        | join(
-                                            ch_split_links
-                                            | map { target_on_xref, seq_tag, txt ->
-                                                [ "${target_on_xref}.${seq_tag}", txt ]
-                                            }
-                                        )
+    ch_circos_inputs                    = ( plot_type in [ 'circos', 'both' ] )
+                                        ? ch_split_links
+                                        | map { target_on_xref, seq_tag, txt ->
+                                            [ "${target_on_xref}.${seq_tag}", txt ]
+                                        }
+                                        | join(GENERATEKARYOTYPE.out.karyotype)
+                                        : Channel.empty()
     CIRCOS ( ch_circos_inputs )
 
     ch_versions                         = ch_versions.mix(CIRCOS.out.versions.first())
 
+    // MODULE: LINEARSYNTENY
+    ch_linear_synteny_inputs            = ( plot_type in [ 'linear', 'both' ] )
+                                        ? ch_split_links
+                                        | map { target_on_xref, seq_tag, txt ->
+                                            [ "${target_on_xref}.${seq_tag}", txt ]
+                                        }
+                                        | join(GENERATEKARYOTYPE.out.karyotype_ref)
+                                        | join(GENERATEKARYOTYPE.out.karyotype_target)
+                                        : Channel.empty()
+
+    LINEARSYNTENY ( ch_linear_synteny_inputs )
+
+    ch_versions                         = ch_versions.mix(LINEARSYNTENY.out.versions.first())
+
     emit:
-    plot                                = CIRCOS.out.png_file
+    png                                 = CIRCOS.out.png_file
+    html                                = LINEARSYNTENY.out.html
     versions                            = ch_versions
 }
 
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index efb6cf15..d709575d 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -366,10 +366,11 @@ workflow ASSEMBLYQC {
         params.synteny_max_gap,
         params.synteny_min_bundle_size,
         params.synteny_plot_1_vs_all,
-        params.synteny_color_by_contig
+        params.synteny_color_by_contig,
+        params.synteny_plot_type
     )
 
-    ch_synteny_plot                         = FASTA_SYNTENY.out.plot
+    ch_synteny_plots                        = FASTA_SYNTENY.out.png.mix(FASTA_SYNTENY.out.html)
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
     // Collate and save software versions
@@ -400,7 +401,7 @@ workflow ASSEMBLYQC {
         ch_lai_outputs                      .collect().ifEmpty([]),
         ch_kraken2_plot                     .collect().ifEmpty([]),
         ch_hic_html                         .collect().ifEmpty([]),
-        ch_synteny_plot                     .collect().ifEmpty([]),
+        ch_synteny_plots                    .collect().ifEmpty([]),
         ch_versions_yml,
         ch_params_as_json,
         ch_summary_params_as_json

From 0cc8a8a60f0a5d7d76c70595840dd5fdef03f962 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 2 May 2024 12:53:40 +1200
Subject: [PATCH 24/76] Updated fasta_ltrretriever_lai to fix [ meta, [] ] bug

---
 modules.json                                  |   2 +-
 .../pfr/fasta_ltrretriever_lai/main.nf        |   1 +
 .../fasta_ltrretriever_lai/tests/main.nf.test | 118 +++++++++++-------
 3 files changed, 78 insertions(+), 43 deletions(-)

diff --git a/modules.json b/modules.json
index 09fd711b..f9c0995c 100644
--- a/modules.json
+++ b/modules.json
@@ -81,7 +81,7 @@
                 "pfr": {
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "8088445a270d324cb06e085f18790839c9684754",
                         "installed_by": ["subworkflows"]
                     },
                     "fastq_bwa_mem_samblaster": {
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
index 7bdc2c28..5c392795 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
@@ -32,6 +32,7 @@ workflow FASTA_LTRRETRIEVER_LAI {
                                     | join(
                                         ch_monoploid_seqs ?: Channel.empty()
                                     )
+                                    | filter { meta, tsv, seqs -> seqs } // Cater to channel: [ meta, tsv, [] ]
                                     | map { meta, short_ids_tsv, monoploid_seqs ->
                                         map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs)
                                     }
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
index 2f31d1d1..4eff67ca 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
@@ -18,22 +18,22 @@ nextflow_workflow {
     tag "ltrretriever/lai"
     tag "custom/restoregffids"
 
-    test("actinidia_chinensis-genome_21_fasta_gz") {
-
-        setup {
-            run("GUNZIP") {
-                script "../../../../modules/nf-core/gunzip"
-
-                process {
-                    """
-                    input[0] = [
-                        [ id:'test' ],
-                        file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true)
-                    ]
-                    """
-                }
+    setup {
+        run("GUNZIP") {
+            script "../../../../modules/nf-core/gunzip"
+
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.test_data['actinidia_chinensis']['genome']['genome_1_fasta_gz'], checkIfExists: true)
+                ]
+                """
             }
         }
+    }
+
+    test("actinidia_chinensis-genome_1_fasta_gz") {
 
         when {
             workflow {
@@ -57,23 +57,43 @@ nextflow_workflow {
         }
     }
 
-    test("actinidia_chinensis-genome_21_fasta_gz-with_mono") {
+    test("actinidia_chinensis-genome_1_fasta_gz-with_mono") {
 
-        setup {
-            run("GUNZIP") {
-                script "../../../../modules/nf-core/gunzip"
+        when {
+            workflow {
+                """
+                GUNZIP.out.gunzip.map { meta, fasta ->
+                    def fa          = new File('test.fa')
+                    fa.write(fasta.text.replaceAll('>chr1', '>chr_xxxxxxxxxxxxxxx_1'))
 
-                process {
-                    """
-                    input[0] = [
-                        [ id:'test' ],
-                        file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true)
-                    ]
-                    """
+                    [ meta, fa.toPath() ]
                 }
+                | set { ch_fa }
+
+                def monoploid_seqs  = new File('test.mono.seq.txt')
+                monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1")
+
+                input[0]            = ch_fa
+                input[1]            = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] )
+                input[2]            = false
+                """
             }
         }
 
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') },
+                { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') },
+                { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') },
+                { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 },
+                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }
+            )
+        }
+    }
+
+    test("actinidia_chinensis-genome_1_fasta_gz-with_empty_mono_channel") {
+
         when {
             workflow {
                 """
@@ -85,11 +105,8 @@ nextflow_workflow {
                 }
                 | set { ch_fa }
 
-                def monoploid_seqs  = new File('test.mono.seq.txt')
-                monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1")
-
                 input[0]            = ch_fa
-                input[1]            = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] )
+                input[1]            = ch_fa.map { meta, fasta -> [ meta, [] ] }
                 input[2]            = false
                 """
             }
@@ -107,25 +124,42 @@ nextflow_workflow {
         }
     }
 
-    test("actinidia_chinensis-genome_21_fasta_gz-without_lai") {
+    test("actinidia_chinensis-genome_1_fasta_gz-with_empty_mono") {
 
-        options '-stub'
+        when {
+            workflow {
+                """
+                GUNZIP.out.gunzip.map { meta, fasta ->
+                    def fa          = new File('test.fa')
+                    fa.write(fasta.text.replaceAll('>chr1', '>chr_xxxxxxxxxxxxxxx_1'))
 
-        setup {
-            run("GUNZIP") {
-                script "../../../../modules/nf-core/gunzip"
-
-                process {
-                    """
-                    input[0] = [
-                        [ id:'test' ],
-                        file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true)
-                    ]
-                    """
+                    [ meta, fa.toPath() ]
                 }
+                | set { ch_fa }
+
+                input[0]            = ch_fa
+                input[1]            = []
+                input[2]            = false
+                """
             }
         }
 
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') },
+                { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') },
+                { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') },
+                { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 },
+                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }
+            )
+        }
+    }
+
+    test("actinidia_chinensis-genome_1_fasta_gz-without_lai") {
+
+        options '-stub'
+
         when {
             workflow {
                 """

From e64b0a9b1b757b1c904ee611b793418cf8490d80 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 2 May 2024 12:55:50 +1200
Subject: [PATCH 25/76] Updated CHANGELOG

---
 CHANGELOG.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e5f2036b..6fab2d46 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0 - [01-May-2024]
+## 2.0.0 - [02-May-2024]
 
 ### `Added`
 
@@ -23,6 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 4. Updated `tower.yml`
 5. Updated LICENSE copyright to Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited
 6. `RUNASSEMBLYVISUALIZER` is now single threaded for successful execution on both Linux and MacOS
+7. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]`
 
 ### `Dependencies`
 

From da09f29429967697536c2f959193fe6e1a451fe5 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 3 May 2024 17:04:22 +1200
Subject: [PATCH 26/76] Fixed java memory overflow issues in
 RUNASSEMBLYVISUALIZER

---
 CHANGELOG.md                           |  3 ++-
 modules/local/runassemblyvisualizer.nf | 13 ++++++++++++-
 2 files changed, 14 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 6fab2d46..ea7cb927 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -23,7 +23,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 4. Updated `tower.yml`
 5. Updated LICENSE copyright to Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited
 6. `RUNASSEMBLYVISUALIZER` is now single threaded for successful execution on both Linux and MacOS
-7. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]`
+7. Fixed java memory overflow issues in `RUNASSEMBLYVISUALIZER`
+8. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]`
 
 ### `Dependencies`
 
diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf
index 8a0d6713..5452dfd0 100644
--- a/modules/local/runassemblyvisualizer.nf
+++ b/modules/local/runassemblyvisualizer.nf
@@ -15,11 +15,20 @@ process RUNASSEMBLYVISUALIZER {
     task.ext.when == null || task.ext.when
 
     script:
+    if ( !task.memory ) { error '[RUNASSEMBLYVISUALIZER] Available memory not known. Specify process memory requirements to fix this.' }
+    def avail_mem = (task.memory.giga*0.8).intValue()
     """
     assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g')
     file_name="${agp_assembly_file}"
 
-    /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\
+    cp -r /usr/src/3d-dna/ \\
+        3d-dna
+
+    sed -i \\
+        's/-Xms49152m -Xmx49152m/-Xms${avail_mem}g -Xmx${avail_mem}g/1' \\
+        3d-dna/visualize/juicebox_tools.sh
+
+    3d-dna/visualize/run-assembly-visualizer.sh \\
         -p false \\
         $agp_assembly_file $sorted_links_txt_file
 
@@ -32,6 +41,8 @@ process RUNASSEMBLYVISUALIZER {
     """
 
     stub:
+    if ( !task.memory ) { error '[RUNASSEMBLYVISUALIZER] Available memory not known. Specify process memory requirements to fix this.' }
+    def avail_mem = (task.memory.giga*0.8).intValue()
     """
     assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g')
     touch "\${assembly_tag}.hic"

From 2f42aef0e6dd1d7e84d26c4bf4f516fea9f22764 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 3 May 2024 20:48:45 +1200
Subject: [PATCH 27/76] Updated docs

---
 .nf-core.yml                                  |   3 +-
 CHANGELOG.md                                  |  16 +-
 CITATION.cff                                  |   2 +-
 README.md                                     |   2 +-
 conf/modules.config                           |   8 +
 docs/output.md                                |   4 +-
 modules.json                                  |   6 +-
 modules/local/linearsynteny.nf                |   3 +-
 modules/pfr/bwa/mem/main.nf                   |  31 +++-
 modules/pfr/bwa/mem/meta.yml                  |  18 ++
 modules/pfr/bwa/mem/tests/main.nf.test        | 143 ++++++++++++++-
 modules/pfr/bwa/mem/tests/main.nf.test.snap   | 169 +++++++++++++++++-
 nextflow.config                               |   2 +-
 .../nf-core/utils_nfcore_pipeline/main.nf     |   8 +-
 .../pfr/fastq_bwa_mem_samblaster/main.nf      |   3 +-
 .../tests/main.nf.test                        |  31 ++++
 .../tests/main.nf.test.snap                   |  53 +++++-
 17 files changed, 456 insertions(+), 46 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 5d078e43..55869c38 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -7,12 +7,13 @@ lint:
     - .github/ISSUE_TEMPLATE/config.yml
     - .github/workflows/awstest.yml
     - .github/workflows/awsfulltest.yml
+    - assets/multiqc_config.yml
+    - conf/igenomes.config
   files_unchanged:
     - CODE_OF_CONDUCT.md
     - assets/nf-core-assemblyqc_logo_light.png
     - docs/images/nf-core-assemblyqc_logo_light.png
     - docs/images/nf-core-assemblyqc_logo_dark.png
-    - .github/ISSUE_TEMPLATE/bug_report.yml
     - docs/README.md
     - .github/CONTRIBUTING.md
     - .github/workflows/linting.yml
diff --git a/CHANGELOG.md b/CHANGELOG.md
index ea7cb927..e3d67040 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,17 +3,17 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0 - [02-May-2024]
+## 2.0.0+dev - [03-May-2024]
 
 ### `Added`
 
-1. Updated modules and sub-workflows
-2. Updated nf-core/template to 2.13.1
-3. Removed release-announcements GitHub workflow
-4. Added a list of nf-core contributors
-5. Added a launcher script for local testing `local_assemblyqc`
-6. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links
-7. Added the ability to create linear synteny plot in addition to the circos plot
+1. Updated nf-core/template to 2.13.1
+2. Removed release-announcements GitHub workflow
+3. Added a list of nf-core contributors
+4. Added a launcher script for local testing `local_assemblyqc`
+5. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links
+6. Added the ability to create linear synteny plot in addition to the circos plot
+7. Updated modules and sub-workflows
 
 ### `Fixed`
 
diff --git a/CITATION.cff b/CITATION.cff
index 23125eae..e704741a 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -21,7 +21,7 @@ authors:
   - family-names: "Deng"
     given-names: "Cecilia"
 title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality"
-version: 2.0.0
+version: 2.0.0+dev
 date-released: 2024-02-12
 url: "https://github.com/Plant-Food-Research-Open/assembly_qc"
 doi: 10.5281/zenodo.10647870
diff --git a/README.md b/README.md
index 5332e400..5e520c33 100644
--- a/README.md
+++ b/README.md
@@ -60,7 +60,7 @@ flowchart LR
 - [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences
 - [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification
 - [HIC CONTACT MAP](https://github.com/igvteam/juicebox-web): Alignment and visualisation of HiC data
-- SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer) and [CIRCOS](http://circos.ca/documentation/)
+- SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer), [CIRCOS](http://circos.ca/documentation/) and [PLOTLY](https://plotly.com)
 
 ## Usage
 
diff --git a/conf/modules.config b/conf/modules.config
index 3185b2ad..d7f07a80 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -84,6 +84,14 @@ process {
         ]
     }
 
+    withName: LINEARSYNTENY {
+        publishDir = [
+            path: { "${params.outdir}/synteny/${target_on_ref_seq}" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
+        ]
+    }
+
     withName: '.*:GFF3_VALIDATE:GT_GFF3' {
         ext.args = '-tidy -retainids'
     }
diff --git a/docs/output.md b/docs/output.md
index b215f5c6..a9e77f7f 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -197,9 +197,9 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
   - `*.on.*.*`: Synteny files corresponding to a single contig of the target assembly with respect to all contigs of the reference assembly.
   </details>
 
-Synteny plots are created with Circos which is a tool [facilitating](https://circos.ca) the identification and analysis of similarities and differences arising from comparisons of genomes. The genome-wide alignments are performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and bundled with [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl).
+[Circos](https://circos.ca) and linear synteny plots are created from genome-wide alignments performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl).
 
-<div align="center"><img src="images/synteny.png" alt="AssemblyQC - Synteny plot" width="50%"><hr><em>AssemblyQC - Synteny plot</em></div>
+<div align="center"><img src="images/synteny.png" alt="AssemblyQC - Synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
 
 ### Pipeline information
 
diff --git a/modules.json b/modules.json
index f9c0995c..f8f52b45 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "bwa/mem": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "26eca75b7373d582569353ed70817072f908b2da",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "cat/cat": {
@@ -86,7 +86,7 @@
                     },
                     "fastq_bwa_mem_samblaster": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "258dbf212876e040e0049809ff0f9fc3f032b8d9",
                         "installed_by": ["subworkflows"]
                     },
                     "gff3_validate": {
@@ -171,7 +171,7 @@
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfvalidation_plugin": {
diff --git a/modules/local/linearsynteny.nf b/modules/local/linearsynteny.nf
index 1296a658..c46f6793 100644
--- a/modules/local/linearsynteny.nf
+++ b/modules/local/linearsynteny.nf
@@ -1,9 +1,8 @@
 process LINEARSYNTENY {
     tag "${target_on_ref_seq}"
-    label "process_single"
+    label 'process_single'
 
     container "docker.io/gallvp/python3npkgs:v0.7"
-    publishDir "${params.outdir}/synteny/${target_on_ref_seq}", mode: 'copy'
 
     input:
     tuple val(target_on_ref_seq), path(bundle_file), path(karyotype_ref), path(karyotype_target)
diff --git a/modules/pfr/bwa/mem/main.nf b/modules/pfr/bwa/mem/main.nf
index 54ec0f16..d8bd2815 100644
--- a/modules/pfr/bwa/mem/main.nf
+++ b/modules/pfr/bwa/mem/main.nf
@@ -8,13 +8,17 @@ process BWA_MEM {
         'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' }"
 
     input:
-    tuple val(meta), path(reads)
+    tuple val(meta) , path(reads)
     tuple val(meta2), path(index)
+    tuple val(meta3), path(fasta)
     val   sort_bam
 
     output:
-    tuple val(meta), path("*.bam"), emit: bam
-    path  "versions.yml"          , emit: versions
+    tuple val(meta), path("*.bam")  , emit: bam,    optional: true
+    tuple val(meta), path("*.cram") , emit: cram,   optional: true
+    tuple val(meta), path("*.csi")  , emit: csi,    optional: true
+    tuple val(meta), path("*.crai") , emit: crai,   optional: true
+    path  "versions.yml"            , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,6 +28,13 @@ process BWA_MEM {
     def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def samtools_command = sort_bam ? 'sort' : 'view'
+    def extension = args2.contains("--output-fmt sam")   ? "sam" :
+                    args2.contains("--output-fmt cram")  ? "cram":
+                    sort_bam && args2.contains("-O cram")? "cram":
+                    !sort_bam && args2.contains("-C")    ? "cram":
+                    "bam"
+    def reference = fasta && extension=="cram"  ? "--reference ${fasta}" : ""
+    if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output"
     """
     INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'`
 
@@ -32,7 +43,7 @@ process BWA_MEM {
         -t $task.cpus \\
         \$INDEX \\
         $reads \\
-        | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam -
+        | samtools $samtools_command $args2 ${reference} --threads $task.cpus -o ${prefix}.${extension} -
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -42,9 +53,19 @@ process BWA_MEM {
     """
 
     stub:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def samtools_command = sort_bam ? 'sort' : 'view'
+    def extension = args2.contains("--output-fmt sam")   ? "sam" :
+                    args2.contains("--output-fmt cram")  ? "cram":
+                    sort_bam && args2.contains("-O cram")? "cram":
+                    !sort_bam && args2.contains("-C")    ? "cram":
+                    "bam"
     """
-    touch ${prefix}.bam
+    touch ${prefix}.${extension}
+    touch ${prefix}.csi
+    touch ${prefix}.crai
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/pfr/bwa/mem/meta.yml b/modules/pfr/bwa/mem/meta.yml
index 440fb1f9..1532c261 100644
--- a/modules/pfr/bwa/mem/meta.yml
+++ b/modules/pfr/bwa/mem/meta.yml
@@ -37,6 +37,10 @@ input:
       type: file
       description: BWA genome index files
       pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}"
+  - fasta:
+      type: file
+      description: Reference genome in FASTA format
+      pattern: "*.{fasta,fa}"
   - sort_bam:
       type: boolean
       description: use samtools sort (true) or samtools view (false)
@@ -46,6 +50,18 @@ output:
       type: file
       description: Output BAM file containing read alignments
       pattern: "*.{bam}"
+  - cram:
+      type: file
+      description: Output CRAM file containing read alignments
+      pattern: "*.{cram}"
+  - csi:
+      type: file
+      description: Optional index file for BAM file
+      pattern: "*.{csi}"
+  - crai:
+      type: file
+      description: Optional index file for CRAM file
+      pattern: "*.{crai}"
   - versions:
       type: file
       description: File containing software versions
@@ -53,6 +69,8 @@ output:
 authors:
   - "@drpatelh"
   - "@jeremy1805"
+  - "@matthdsm"
 maintainers:
   - "@drpatelh"
   - "@jeremy1805"
+  - "@matthdsm"
diff --git a/modules/pfr/bwa/mem/tests/main.nf.test b/modules/pfr/bwa/mem/tests/main.nf.test
index 804f23f7..1fa9b56d 100644
--- a/modules/pfr/bwa/mem/tests/main.nf.test
+++ b/modules/pfr/bwa/mem/tests/main.nf.test
@@ -35,7 +35,8 @@ nextflow_process {
                     ]
                 ]
                 input[1] = BWA_INDEX.out.index
-                input[2] = false
+                input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]
+                input[3] = false
                 """
             }
         }
@@ -75,7 +76,8 @@ nextflow_process {
                     ]
                 ]
                 input[1] = BWA_INDEX.out.index
-                input[2] = true
+                input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]
+                input[3] = true
                 """
             }
         }
@@ -116,7 +118,8 @@ nextflow_process {
                     ]
                 ]
                 input[1] = BWA_INDEX.out.index
-                input[2] = false
+                input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]
+                input[3] = false
                 """
             }
         }
@@ -157,7 +160,8 @@ nextflow_process {
                     ]
                 ]
                 input[1] = BWA_INDEX.out.index
-                input[2] = true
+                input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]
+                input[3] = true
                 """
             }
         }
@@ -170,4 +174,133 @@ nextflow_process {
         }
 
     }
-}
\ No newline at end of file
+
+    test("Paired-End - no fasta") {
+
+        setup {
+            run("BWA_INDEX") {
+                script "../../index/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [id: 'test'],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                    ]
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ]
+                input[1] = BWA_INDEX.out.index
+                input[2] = [[:],[]]
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("Single-end - stub") {
+        options "-stub"
+        setup {
+            run("BWA_INDEX") {
+                script "../../index/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [id: 'test'],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                    ]
+                    """
+                }
+            }
+        }
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                    ]
+                ]
+                input[1] = BWA_INDEX.out.index
+                input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("Paired-end - stub") {
+        options "-stub"
+        setup {
+            run("BWA_INDEX") {
+                script "../../index/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [id: 'test'],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                    ]
+                    """
+                }
+            }
+        }
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ]
+                input[1] = BWA_INDEX.out.index
+                input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/modules/pfr/bwa/mem/tests/main.nf.test.snap b/modules/pfr/bwa/mem/tests/main.nf.test.snap
index e4fd8cc0..0d1bdb41 100644
--- a/modules/pfr/bwa/mem/tests/main.nf.test.snap
+++ b/modules/pfr/bwa/mem/tests/main.nf.test.snap
@@ -12,6 +12,15 @@
                     ]
                 ],
                 "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
                 ],
                 "bam": [
@@ -22,6 +31,15 @@
                         },
                         "test.bam:md5,a74710a0345b4717bb4431bf9c257120"
                     ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
@@ -30,9 +48,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-19T11:11:48.440661587"
+        "timestamp": "2024-03-14T14:14:56.820798254"
     },
     "Single-End Sort": {
         "content": [
@@ -47,6 +65,15 @@
                     ]
                 ],
                 "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
                 ],
                 "bam": [
@@ -57,6 +84,15 @@
                         },
                         "test.bam:md5,cb1e038bc4d990683fa485d632550b54"
                     ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
@@ -65,9 +101,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-19T11:11:56.086493265"
+        "timestamp": "2024-03-14T14:15:20.271428534"
     },
     "Paired-End": {
         "content": [
@@ -82,6 +118,15 @@
                     ]
                 ],
                 "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
                 ],
                 "bam": [
@@ -92,6 +137,15 @@
                         },
                         "test.bam:md5,aea123a3828a99da1906126355f15a12"
                     ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
@@ -100,9 +154,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-19T11:12:03.474974773"
+        "timestamp": "2024-03-14T14:15:38.264256823"
     },
     "Paired-End Sort": {
         "content": [
@@ -117,6 +171,15 @@
                     ]
                 ],
                 "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
                 ],
                 "bam": [
@@ -127,6 +190,82 @@
                         },
                         "test.bam:md5,4682087bcdc3617384b375093fecd8dd"
                     ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-14T14:16:00.528642686"
+    },
+    "Single-end - stub": {
+        "content": [
+            "test.bam",
+            "test.csi",
+            [
+                "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-15T09:50:33.238543011"
+    },
+    "Paired-End - no fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,aea123a3828a99da1906126355f15a12"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,aea123a3828a99da1906126355f15a12"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
@@ -135,8 +274,22 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-14T14:40:16.345342005"
+    },
+    "Paired-end - stub": {
+        "content": [
+            "test.bam",
+            "test.csi",
+            [
+                "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-19T11:12:10.721510817"
+        "timestamp": "2024-03-15T09:50:42.268673302"
     }
 }
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 9aa0e97b..6fa14fdb 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -264,7 +264,7 @@ manifest {
     description     = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '2.0.0'
+    version         = '2.0.0+dev'
     doi             = '10.5281/zenodo.10647870'
 }
 
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index a8b55d6f..14558c39 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) {
 // Citation string for pipeline
 //
 def workflowCitation() {
+    def temp_doi_ref = ""
+    String[] manifest_doi = workflow.manifest.doi.tokenize(",")
+    // Using a loop to handle multiple DOIs
+    // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
+    // Removing ` ` since the manifest.doi is a string and not a proper list
+    for (String doi_ref: manifest_doi) temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
     return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
         "* The pipeline\n" +
-        "  ${workflow.manifest.doi}\n\n" +
+        temp_doi_ref + "\n" +
         "* The nf-core framework\n" +
         "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
         "* Software dependencies\n" +
diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf b/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf
index 50e824a9..456f36c7 100644
--- a/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf
+++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf
@@ -6,7 +6,7 @@ workflow FASTQ_BWA_MEM_SAMBLASTER {
 
     take:
     ch_fastq                // channel: [ val(meta), [ fq ] ]
-    ch_reference            // channel: [ val(meta2), fasta, index ]; fast | index
+    ch_reference            // channel: [ val(meta2), fasta, index ]; fasta | index
 
     main:
     ch_versions             = Channel.empty()
@@ -43,6 +43,7 @@ workflow FASTQ_BWA_MEM_SAMBLASTER {
     BWA_MEM(
         ch_mem_inputs.map { meta, fq, index -> [ meta, fq ] },
         ch_mem_inputs.map { meta, fq, index -> [ [], index ] },
+        [ [], [] ],
         sort_bam
     )
 
diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test
index 9c3e2b01..0a84fd8c 100644
--- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test
+++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test
@@ -41,4 +41,35 @@ nextflow_workflow {
             )
         }
     }
+
+    test("sarscov2-fq-gz-stub") {
+
+        options '-stub'
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of(
+                    [
+                        [ id:'test' ],
+                        [
+                            file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                        ]
+                    ]
+                )
+                input[1] = Channel.of(
+                    [ [ id: 'genome' ], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), [] ]
+                )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match()}
+            )
+        }
+    }
 }
\ No newline at end of file
diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap
index e5a9b18e..83f6bc6d 100644
--- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap
+++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap
@@ -8,12 +8,12 @@
                             "id": "test",
                             "ref_id": "genome"
                         },
-                        "test.on.genome.samblaster.bam:md5,496319fc81c383a9ae6ab52592876c9b"
+                        "test.on.genome.samblaster.bam:md5,8c47b2669f5250ad2d24365b42566d78"
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,2ed54ca5e54063cb579273d0792465a7",
-                    "versions.yml:md5,49d22cda9beaf6ea1a1ad838ef4a4255",
+                    "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
+                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
                     "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
                 ],
                 "bam": [
@@ -22,12 +22,12 @@
                             "id": "test",
                             "ref_id": "genome"
                         },
-                        "test.on.genome.samblaster.bam:md5,496319fc81c383a9ae6ab52592876c9b"
+                        "test.on.genome.samblaster.bam:md5,8c47b2669f5250ad2d24365b42566d78"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2ed54ca5e54063cb579273d0792465a7",
-                    "versions.yml:md5,49d22cda9beaf6ea1a1ad838ef4a4255",
+                    "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
+                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
                     "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
                 ]
             }
@@ -36,6 +36,45 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-08T19:37:23.464854"
+        "timestamp": "2024-05-03T15:58:33.221762"
+    },
+    "sarscov2-fq-gz-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "ref_id": "genome"
+                        },
+                        "test.on.genome.samblaster.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
+                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
+                    "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "ref_id": "genome"
+                        },
+                        "test.on.genome.samblaster.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
+                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
+                    "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T16:03:28.825915"
     }
 }
\ No newline at end of file

From 23017999db6ad6ca865e4d2782a763c4f9d9a6a9 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 6 May 2024 11:44:01 +1200
Subject: [PATCH 28/76] Updated docs

---
 docs/images/synteny.png        | Bin 305494 -> 0 bytes
 docs/images/synteny_circos.png | Bin 0 -> 339304 bytes
 docs/images/synteny_linear.png | Bin 0 -> 108201 bytes
 docs/output.md                 |  16 +++++++++-------
 4 files changed, 9 insertions(+), 7 deletions(-)
 delete mode 100644 docs/images/synteny.png
 create mode 100644 docs/images/synteny_circos.png
 create mode 100644 docs/images/synteny_linear.png

diff --git a/docs/images/synteny.png b/docs/images/synteny.png
deleted file mode 100644
index edd8bc4ac73120f9913767efc2943bd0cfd22b7a..0000000000000000000000000000000000000000
GIT binary patch
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zKUAWvdZlIng~9UFkuv;EjWo<vi_u=Fw(Pfj$tWJu)y)N6i@^qV=AR0}WxU>|0DIM4
z+85Mep>L7%PUxB4aT+LU<KDc5F*M^Di8*me!?SJeCc_E3mJau93$%vKFNUw<))@D-
zk;*d@CL|AL#^%-3{6@To$N?c_vW`tFeBrb^*#^kk1+D6yoiEBKW<Fd&D9SVGyk<8)
zZS6l@S>?A$RGKN}1CpTZ#K2{cq>~SM%iJnsum$Z(VuX6Jq)PWJu`T--1EtPp>$^h7
z-ZNtW$7Y;Ozqq(o7>DbWofOPgkEyA$ncN*#Z%*+By34u12OS>+e<oELxzyxM;)DdS
zZy(VX)^D9GYX1_v*qVoz;JOCu{A01ZnT%XvhN)zzf{SzfAB&mR1wxL!M{94dpv<=l
z4Pt$qs?XJ~eVF=dTkT&wHV^>|sOKcT9|<DT&wZwsL%%8PeQbRE8g5tV!M^Z)g(p3N
zci(iizi0Q3Wp*fZ@M4ko=}WB4BK9E!W4?U(u~)uTO@WmSP=eQ)J}zn&mYP3^lJ=AL
z$c~FcS=$DxARh$g^$#P=%vJ)}VNgYENmFh=Q()U)^9bt%q<@M$%*SVqlC^&}y?~O3
z7!=`)(e9>He%L#~@+bFgx!`^#TiH3Zja-sC;aj(hHD#shT6$VygUz5vxvb{^@A8Iv
zP<*BGzI+#+7TyCft{09!uzMrV@*`Pdj8XZX13ReoBReVlH28axN8>0ngLefK#F=03
z-t~j_LGSqif?PfH1C{vW)DOww|9{c{?Q1m7U6E!};9?gN!>}D)EyJq?zukWLU$E!A
AZ2$lO

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HcmV?d00001

diff --git a/docs/output.md b/docs/output.md
index a9e77f7f..8115e697 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -109,7 +109,7 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c
 
 - `tidk/`
   - `*.apriori.tsv`: Frequencies for successive windows in forward and reverse directions for the pre-specified telomere-repeat sequence.
-  - `*.apriori.svg`: Plot of `*.apriori.tsv`.
+  - `*.apriori.svg`: Plot of `*.apriori.tsv`
   - `*.tidk.explore.tsv`: List of the most frequent repeat sequences.
   - `*.top.sequence.txt`: The top sequence from `*.tidk.explore.tsv`.
   - `*.aposteriori.tsv`: Frequencies for successive windows in forward and reverse directions for the top sequence from `*.top.sequence.txt`.
@@ -170,12 +170,12 @@ Kraken2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to seq
     - `*_1.fastp.fastq.gz/*_2.fastp.fastq.gz`: Reads passed by FASTP
     - `*_1.fail.fastq.gz/*_2.fail.fastq.gz`: Reads failed by FASTP
   - `fastqc_trim/`
-    - `*_1_fastqc.html/*_2_fastqc.html`: FASTQC html report for the reads passed by FASTP
-    - `*_1_fastqc.zip/*_2_fastqc.zip`: FASTQC stats for the reads passed by FASTP
+    - `*_1_fastqc.html/*_2_fastqc.html`: FASTQC html report for the reads passed by FASTP.
+    - `*_1_fastqc.zip/*_2_fastqc.zip`: FASTQC stats for the reads passed by FASTP.
   - `hicqc`
     - `*.on.*_qc_report.pdf`: HiC QC report for reads mapped to an assembly.
   - `assembly/`
-    - `*.agp.assembly`: AGP assembly file listing the length of each contig in the assembly
+    - `*.agp.assembly`: AGP assembly file listing the length of each contig in the assembly.
   - `bedpe/` - `*.assembly.bedpe`: `*.agp.assembly` file converted to BEDPE to highlight the contigs on the HiC contact map.
   </details>
 
@@ -191,7 +191,8 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 - `synteny/`
   - `*.*.all/`: Synteny files corresponding to all contigs of the target assembly with respect to all contig of the reference assembly.
     - `*.on.*.all.png/svg`: Synteny plot generated with CIRCOS.
-    - `bundled.links.tsv`: Bundled links file generated with MUMMER and `dnadiff.pl`.
+    - `*.on.*.all.png/svg`: Synteny plot generated with Plotly.
+    - `bundled.links.tsv`: Bundled links file generated with MUMMER, `MUMMER/dnadiff.pl` and [bundlelinks.py](../bin/bundlelinks.py).
     - `circos.conf`: CIRCOS configuration file used to generate the synteny plot.
     - `karyotype.tsv`: Karyotype TSV file used to generate the synteny plot.
   - `*.on.*.*`: Synteny files corresponding to a single contig of the target assembly with respect to all contigs of the reference assembly.
@@ -199,7 +200,8 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 
 [Circos](https://circos.ca) and linear synteny plots are created from genome-wide alignments performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl).
 
-<div align="center"><img src="images/synteny.png" alt="AssemblyQC - Synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
+<div align="center"><img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
+<div align="center"><img src="images/synteny_linear.png" alt="AssemblyQC - Linear synteny plot" width="50%"><hr><em>AssemblyQC - Linear synteny plot</em></div>
 
 ### Pipeline information
 
@@ -209,7 +211,7 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 - `pipeline_info/`
   - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.html`.
   - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
-  - Parameters used by the pipeline run: `params.json`.
+  - Parameters used by the pipeline run: `params.json`
 
 </details>
 

From 7e9872e2961ea310b25672881516dbd34c7813d7 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 6 May 2024 12:18:01 +1200
Subject: [PATCH 29/76] Now pipeline can continue if contamination is detected

---
 CHANGELOG.md            | 13 +++++++------
 docs/parameters.md      | 15 ++++++++-------
 docs/usage.md           |  4 +++-
 nextflow.config         |  2 ++
 nextflow_schema.json    | 42 +++++++++++++++++++++++++++++------------
 workflows/assemblyqc.nf |  8 ++++++--
 6 files changed, 56 insertions(+), 28 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e3d67040..32347afa 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0+dev - [03-May-2024]
+## 2.0.0+dev - [06-May-2024]
 
 ### `Added`
 
@@ -11,20 +11,21 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 2. Removed release-announcements GitHub workflow
 3. Added a list of nf-core contributors
 4. Added a launcher script for local testing `local_assemblyqc`
-5. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links
-6. Added the ability to create linear synteny plot in addition to the circos plot
+5. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
+6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
 7. Updated modules and sub-workflows
+8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
 
 ### `Fixed`
 
-1. Fixed a bug which caused NCBI_FCS_GX to not resume
+1. Fixed a bug which caused NCBI_FCS_GX to not resume [#80](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/80)
 2. Restored the original version of `nf-core/subworkflows/fastq_trim_fastp_fastqc`
 3. Fixed n-core linting
 4. Updated `tower.yml`
-5. Updated LICENSE copyright to Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited
+5. Updated LICENSE copyright to Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited [#81](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/81)
 6. `RUNASSEMBLYVISUALIZER` is now single threaded for successful execution on both Linux and MacOS
 7. Fixed java memory overflow issues in `RUNASSEMBLYVISUALIZER`
-8. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]`
+8. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]` [#83](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/83)
 
 ### `Dependencies`
 
diff --git a/docs/parameters.md b/docs/parameters.md
index dd749c5b..e9a4df43 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -18,13 +18,14 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## NCBI FCS options
 
-| Parameter                 | Description                                                                           | Type      | Default | Required | Hidden |
-| ------------------------- | ------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
-| `ncbi_fcs_adaptor_skip`   | Skip NCBI FCS Adaptor checking                                                        | `boolean` | True    |          |        |
-| `ncbi_fcs_adaptor_empire` | Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes | `string`  |         |          |        |
-| `ncbi_fcs_gx_skip`        | Skip NCBI FCS external organism contamination checking                                | `boolean` | True    |          |        |
-| `ncbi_fcs_gx_tax_id`      | Tax ID for NCBI FCS GX. See: https://www.ncbi.nlm.nih.gov/taxonomy                    | `number`  |         |          |        |
-| `ncbi_fcs_gx_db_path`     | Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX            | `string`  |         |          |        |
+| Parameter                      | Description                                                                           | Type      | Default | Required | Hidden |
+| ------------------------------ | ------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `ncbi_fcs_adaptor_skip`        | Skip NCBI FCS Adaptor checking                                                        | `boolean` | True    |          |        |
+| `ncbi_fcs_adaptor_empire`      | Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes | `string`  |         |          |        |
+| `ncbi_fcs_gx_skip`             | Skip NCBI FCS external organism contamination checking                                | `boolean` | True    |          |        |
+| `ncbi_fcs_gx_tax_id`           | Tax ID for NCBI FCS GX. See: https://www.ncbi.nlm.nih.gov/taxonomy                    | `number`  |         |          |        |
+| `ncbi_fcs_gx_db_path`          | Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX            | `string`  |         |          |        |
+| `contamination_stops_pipeline` | Skip remaining QC steps for an assembly which has adaptor or GX contamination         | `boolean` | True    |          |        |
 
 ## BUSCO options
 
diff --git a/docs/usage.md b/docs/usage.md
index 1f2bd37c..0f4214ef 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -48,7 +48,8 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
 
 ### NCBI FCS GX
 
-`ncbi_fcs_gx_tax_id` is the taxonomy ID for all the assemblies listed in the assemblysheet. A taxonomy ID can be obtained by searching a _Genus species_ at <https://www.ncbi.nlm.nih.gov/taxonomy>.
+- `ncbi_fcs_gx_tax_id` is the taxonomy ID for all the assemblies listed in the assemblysheet. A taxonomy ID can be obtained by searching a _Genus species_ at <https://www.ncbi.nlm.nih.gov/taxonomy>.
+- `contamination_stops_pipeline` when set to `true`, skips the remaining QC steps for an assembly which has adaptor or GX contamination.
 
 ### BUSCO
 
@@ -70,6 +71,7 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
 
 ### Synteny analysis
 
+- `synteny_plot_type`: Set it to `linear`, `circos`, or `both` to generate CIRCOS or linear or both synteny plots.
 - `synteny_between_input_assemblies`: Set it to `true` to create synteny plots between each pair of input assemblies. Default is `true`.
 - `synteny_many_to_many_align`: Set it to `true` to include alignment blocks with many-to-many mappings or set to `false` to only include 1-to-1 mappings. Default is `false`. See the documentation of `dnadiff` for further details: <https://github.com/mummer4/mummer/blob/master/docs/dnadiff.README>
 - `synteny_max_gap`: Alignments within this distance are bundled together. Default: 1000000 (1 Mbp).
diff --git a/nextflow.config b/nextflow.config
index 6fa14fdb..5a8128aa 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -23,6 +23,8 @@ params {
     ncbi_fcs_gx_tax_id                  = null
     ncbi_fcs_gx_db_path                 = null
 
+    contamination_stops_pipeline        = true
+
     // BUSCO options
     busco_skip                          = true
     busco_mode                          = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 6e683bfb..432f3f82 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -59,7 +59,8 @@
                 "ncbi_fcs_adaptor_skip": {
                     "type": "boolean",
                     "description": "Skip NCBI FCS Adaptor checking",
-                    "default": true
+                    "default": true,
+                    "fa_icon": "fas fa-forward"
                 },
                 "ncbi_fcs_adaptor_empire": {
                     "type": "string",
@@ -69,7 +70,8 @@
                 "ncbi_fcs_gx_skip": {
                     "type": "boolean",
                     "description": "Skip NCBI FCS external organism contamination checking",
-                    "default": true
+                    "default": true,
+                    "fa_icon": "fas fa-forward"
                 },
                 "ncbi_fcs_gx_tax_id": {
                     "type": "number",
@@ -78,7 +80,14 @@
                 "ncbi_fcs_gx_db_path": {
                     "type": "string",
                     "format": "directory-path",
-                    "description": "Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX"
+                    "description": "Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "contamination_stops_pipeline": {
+                    "type": "boolean",
+                    "default": true,
+                    "fa_icon": "fas fa-hand-paper",
+                    "description": "Skip remaining QC steps for an assembly which has adaptor or GX contamination"
                 }
             }
         },
@@ -91,7 +100,8 @@
                 "busco_skip": {
                     "type": "boolean",
                     "description": "Skip BUSCO",
-                    "default": true
+                    "default": true,
+                    "fa_icon": "fas fa-forward"
                 },
                 "busco_mode": {
                     "type": "string",
@@ -106,7 +116,8 @@
                 "busco_download_path": {
                     "type": "string",
                     "description": "Download path for BUSCO",
-                    "format": "directory-path"
+                    "format": "directory-path",
+                    "fa_icon": "fas fa-folder-open"
                 }
             }
         },
@@ -119,7 +130,8 @@
                 "tidk_skip": {
                     "type": "boolean",
                     "description": "Skip telomere identification",
-                    "default": true
+                    "default": true,
+                    "fa_icon": "fas fa-forward"
                 },
                 "tidk_repeat_seq": {
                     "type": "string",
@@ -146,7 +158,8 @@
                 "lai_skip": {
                     "type": "boolean",
                     "default": true,
-                    "description": "Skip LAI estimation"
+                    "description": "Skip LAI estimation",
+                    "fa_icon": "fas fa-forward"
                 }
             }
         },
@@ -159,12 +172,14 @@
                 "kraken2_skip": {
                     "type": "boolean",
                     "default": true,
-                    "description": "Skip Kraken2"
+                    "description": "Skip Kraken2",
+                    "fa_icon": "fas fa-forward"
                 },
                 "kraken2_db_path": {
                     "type": "string",
                     "description": "Kraken2 database path",
-                    "format": "path"
+                    "format": "path",
+                    "fa_icon": "fas fa-folder-open"
                 }
             }
         },
@@ -181,11 +196,13 @@
                 },
                 "hic_skip_fastp": {
                     "type": "boolean",
-                    "description": "Skip HiC read trimming"
+                    "description": "Skip HiC read trimming",
+                    "fa_icon": "fas fa-forward"
                 },
                 "hic_skip_fastqc": {
                     "type": "boolean",
-                    "description": "Skip HiC read QC"
+                    "description": "Skip HiC read QC",
+                    "fa_icon": "fas fa-forward"
                 },
                 "hic_fastp_ext_args": {
                     "type": "string",
@@ -203,7 +220,8 @@
                 "synteny_skip": {
                     "type": "boolean",
                     "default": true,
-                    "description": "Skip synteny analysis"
+                    "description": "Skip synteny analysis",
+                    "fa_icon": "fas fa-forward"
                 },
                 "synteny_plot_type": {
                     "type": "string",
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index d709575d..7a6e7243 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -172,7 +172,9 @@ workflow ASSEMBLYQC {
                                                 | map { tag, report ->
                                                     [ tag, file(report).readLines().size < 2 ]
                                                 }
-                                                | filter { tag, is_clean -> is_clean }
+                                                | filter { tag, is_clean ->
+                                                    ( is_clean || ( ! params.contamination_stops_pipeline ) )
+                                                }
                                                 | join(
                                                     ch_valid_target_assembly
                                                     | map { meta, fa -> [ meta.id, fa ] }
@@ -223,7 +225,9 @@ workflow ASSEMBLYQC {
                                                 | map { tag, report ->
                                                     [ tag, file(report).readLines().size < 3 ]
                                                 }
-                                                | filter { tag, is_clean -> is_clean }
+                                                | filter { tag, is_clean ->
+                                                    ( is_clean || ( ! params.contamination_stops_pipeline ) )
+                                                }
                                                 | join(
                                                     ch_valid_target_assembly
                                                     | map { meta, fa -> [ meta.id, fa ] }

From eaa0cf9a2528c6a01f42ea32124b47a3cbe96683 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 6 May 2024 13:14:52 +1200
Subject: [PATCH 30/76] Updated fcs-adaptor to 0.5.0

---
 CHANGELOG.md                      |  1 +
 modules/local/ncbi_fcs_adaptor.nf | 10 +++++-----
 2 files changed, 6 insertions(+), 5 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 32347afa..14cfc474 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
 7. Updated modules and sub-workflows
 8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
+9. Updated `fcs-adaptor` to 0.5.0 [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
 
 ### `Fixed`
 
diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf
index ec1b3dd0..3db1b3ba 100644
--- a/modules/local/ncbi_fcs_adaptor.nf
+++ b/modules/local/ncbi_fcs_adaptor.nf
@@ -4,8 +4,8 @@ process NCBI_FCS_ADAPTOR {
 
     // Warning: manually update version in script and stub
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-adaptor.sif':
-        'docker.io/ncbi/fcs-adaptor:0.4.0' }"
+        'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.5.0/fcs-adaptor.sif':
+        'docker.io/ncbi/fcs-adaptor:0.5.0' }"
 
     input:
     tuple val(asm_tag), path(fasta_file)
@@ -23,11 +23,11 @@ process NCBI_FCS_ADAPTOR {
     if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
         error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead."
     }
-    def VERSION = 0.4
+    def VERSION = 0.5
     """
     mkdir "${asm_tag}_outputdir"
 
-    /app/fcs/bin/av_screen_x \\
+    av_screen_x \\
         -o "${asm_tag}_outputdir" \\
         --${empire} \\
         "${fasta_file}"
@@ -46,7 +46,7 @@ process NCBI_FCS_ADAPTOR {
     if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
         error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead."
     }
-    def VERSION = 0.4
+    def VERSION = 0.5
     """
     touch "${asm_tag}_fcs_adaptor_report.tsv"
 

From 50a0b5f4e9bd9a69dc9bf2478778900efb8ac0f4 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 6 May 2024 16:31:20 +1200
Subject: [PATCH 31/76] Now fasta ids are sorted in natural order for

---
 CHANGELOG.md                 |  1 +
 conf/modules.config          |  4 ++++
 subworkflows/local/fq2hic.nf | 11 +++++++++--
 3 files changed, 14 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 14cfc474..7541902a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 7. Updated modules and sub-workflows
 8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
 9. Updated `fcs-adaptor` to 0.5.0 [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
+10. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index d7f07a80..c532b809 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -240,6 +240,10 @@ process {
         ]
     }
 
+    withName: '.*:FQ2HIC:SEQKIT_SORT' {
+        ext.args = '--ignore-case --natural-order'
+    }
+
     withName: BWA_MEM {
         ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" }
         ext.args = '-5SP'
diff --git a/subworkflows/local/fq2hic.nf b/subworkflows/local/fq2hic.nf
index f8a3eaa9..38761473 100644
--- a/subworkflows/local/fq2hic.nf
+++ b/subworkflows/local/fq2hic.nf
@@ -1,5 +1,6 @@
 include { FASTQ_TRIM_FASTP_FASTQC   } from '../nf-core/fastq_trim_fastp_fastqc/main'
 include { FASTQ_BWA_MEM_SAMBLASTER  } from '../pfr/fastq_bwa_mem_samblaster/main'
+include { SEQKIT_SORT               } from '../../modules/nf-core/seqkit/sort/main'
 include { HICQC                     } from '../../modules/local/hicqc'
 include { MAKEAGPFROMFASTA          } from '../../modules/local/makeagpfromfasta'
 include { AGP2ASSEMBLY              } from '../../modules/local/agp2assembly'
@@ -32,10 +33,16 @@ workflow FQ2HIC {
     ch_trim_reads                   = FASTQ_TRIM_FASTP_FASTQC.out.reads
     ch_versions                     = ch_versions.mix(FASTQ_TRIM_FASTP_FASTQC.out.versions)
 
+    // MODULE: SEQKIT_SORT
+    SEQKIT_SORT ( ref )
+
+    ch_sorted_ref                   = SEQKIT_SORT.out.fastx
+    ch_versions                     = ch_versions.mix(SEQKIT_SORT.out.versions)
+
     // SUBWORKFLOW: FASTQ_BWA_MEM_SAMBLASTER
     FASTQ_BWA_MEM_SAMBLASTER(
         ch_trim_reads,
-        ref.map { meta2, fa -> [ meta2, fa, [] ] }
+        ch_sorted_ref.map { meta2, fa -> [ meta2, fa, [] ] }
     )
 
     ch_bam                          = FASTQ_BWA_MEM_SAMBLASTER.out.bam
@@ -45,7 +52,7 @@ workflow FQ2HIC {
     ch_bam_and_ref                  = ch_bam
                                     | map { meta, bam -> [ meta.ref_id, meta, bam ] }
                                     | join(
-                                        ref.map { meta2, fa -> [ meta2.id, fa ] }
+                                        ch_sorted_ref.map { meta2, fa -> [ meta2.id, fa ] }
                                     )
                                     | map { ref_id, meta, bam, fa ->
                                         [ [ id: "${meta.id}.on.${meta.ref_id}" ], bam, fa ]

From fc858a27fe394f1f75101816f28be531d6efda60 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 7 May 2024 17:04:49 +1200
Subject: [PATCH 32/76] Added merqury params to schema_input

---
 README.md                |  6 ++----
 assets/schema_input.json | 41 ++++++++++++++++++++++++++++++++++++++++
 pfr/params.json          | 11 +++++++++--
 3 files changed, 52 insertions(+), 6 deletions(-)

diff --git a/README.md b/README.md
index 5e520c33..0b73d8ae 100644
--- a/README.md
+++ b/README.md
@@ -69,13 +69,10 @@ Refer to [usage](./docs/usage.md), [parameters](./docs/parameters.md) and [outpu
 > [!NOTE]
 > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
 
-Prepare an `assemblysheet.csv` file with following columns representing target assemblies and associated meta-data. See an example [assemblysheet.csv](./assets/assemblysheet.csv)
+Prepare an `assemblysheet.csv` file with following columns representing target assemblies and associated meta-data.
 
 - `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report
 - `fasta:` FASTA file
-- `gff3 [Optional]:` GFF3 annotation file if available
-- `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
-- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis
 
 Now, you can run the pipeline using:
 
@@ -109,6 +106,7 @@ We thank the following people for their extensive assistance in the development
 - Jason Shiller [@jasonshiller](https://github.com/jasonshiller)
 - Marcus Davy [@mdavy86](https://github.com/mdavy86)
 - Ross Crowhurst [@rosscrowhurst](https://github.com/rosscrowhurst)
+- Sarah Bailey [@SarahBailey1998](https://github.com/SarahBailey1998)
 - Susan Thomson [@cflsjt](https://github.com/cflsjt)
 - Ting-Hsuan Chen [@ting-hsuan-chen](https://github.com/ting-hsuan-chen)
 
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 2aab4188..1fd88892 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -47,6 +47,47 @@
                         "maxLength": 0
                     }
                 ]
+            },
+            "reads": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ]
+            },
+            "maternal_reads": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ],
+                "dependentRequired": ["reads", "paternal_reads"]
+            },
+            "paternal_reads": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ],
+                "dependentRequired": ["reads", "maternal_reads"]
             }
         },
         "required": ["tag", "fasta"]
diff --git a/pfr/params.json b/pfr/params.json
index ab1ea360..d6f32ff5 100644
--- a/pfr/params.json
+++ b/pfr/params.json
@@ -1,10 +1,12 @@
 {
     "input": "/workspace/assemblyqc/testdata/default/assemblysheet.csv",
+    "assemblathon_stats_n_limit": 100,
     "ncbi_fcs_adaptor_skip": false,
     "ncbi_fcs_adaptor_empire": "euk",
     "ncbi_fcs_gx_skip": false,
     "ncbi_fcs_gx_tax_id": 3750,
     "ncbi_fcs_gx_db_path": "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24",
+    "contamination_stops_pipeline": true,
     "busco_skip": false,
     "busco_mode": "geno",
     "busco_lineage_datasets": "embryophyta_odb10 eudicots_odb10",
@@ -17,13 +19,18 @@
     "kraken2_skip": false,
     "kraken2_db_path": "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314",
     "hic": null,
+    "hic_skip_fastp": false,
+    "hic_skip_fastqc": false,
+    "hic_fastp_ext_args": "--qualified_quality_phred 20 --length_required 50",
     "synteny_skip": false,
+    "synteny_plot_type": "both",
     "synteny_between_input_assemblies": true,
     "synteny_many_to_many_align": false,
     "synteny_max_gap": 1000000,
     "synteny_min_bundle_size": 1000,
-    "synteny_plot_1_vs_all": true,
+    "synteny_plot_1_vs_all": false,
     "synteny_color_by_contig": true,
     "synteny_xref_assemblies": "/workspace/assemblyqc/testdata/default/xrefsheet.csv",
-    "outdir": "./results"
+    "outdir": "./results",
+    "email": null
 }

From 45d180e020c77cefe2409adddf09ee77b0e9b3ff Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 7 May 2024 20:55:57 +1200
Subject: [PATCH 33/76] Expanded schema_input

---
 assets/schema_input.json                      | 52 +++++++++++++++++--
 .../utils_nfcore_assemblyqc_pipeline/main.nf  | 35 +++++++------
 workflows/assemblyqc.nf                       | 20 +++++--
 3 files changed, 82 insertions(+), 25 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 1fd88892..cd19ce8b 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -48,7 +48,7 @@
                     }
                 ]
             },
-            "reads": {
+            "reads_1": {
                 "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
@@ -61,7 +61,7 @@
                     }
                 ]
             },
-            "maternal_reads": {
+            "reads_2": {
                 "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
@@ -73,9 +73,9 @@
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["reads", "paternal_reads"]
+                "dependentRequired": ["reads_1"]
             },
-            "paternal_reads": {
+            "maternal_reads_1": {
                 "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
@@ -87,7 +87,49 @@
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["reads", "maternal_reads"]
+                "dependentRequired": ["reads_1", "paternal_reads_1"]
+            },
+            "maternal_reads_2": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ],
+                "dependentRequired": ["maternal_reads_1"]
+            },
+            "paternal_reads_1": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ],
+                "dependentRequired": ["reads_1", "maternal_reads_1"]
+            },
+            "paternal_reads_2": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ],
+                "dependentRequired": ["paternal_reads_1"]
             }
         },
         "required": ["tag", "fasta"]
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 95702cc6..978ad199 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -83,13 +83,16 @@ workflow PIPELINE_INITIALISATION {
     //
     // Initialise input channels
     //
-    def input_assembly_sheet_fields         = 5
-    def synteny_xref_assemblies_fields      = 3
 
     ch_input                                = Channel.fromSamplesheet('input')
+
+    // Function: validateInputTags
+    ch_input_validated                      = ch_input
+                                            | map { row -> row[0] }
                                             | collect
-                                            | flatMap { validateInput(it) }
-                                            | buffer(size: input_assembly_sheet_fields)
+                                            | map { tags -> validateInputTags( tags ) }
+                                            | combine ( ch_input.map { row -> [ row ] } )
+                                            | map { result, row -> row }
 
     ch_hic_reads                            = ! params.hic
                                             ? Channel.empty()
@@ -105,9 +108,13 @@ workflow PIPELINE_INITIALISATION {
     ch_xref_assembly                        = params.synteny_skip || ! params.synteny_xref_assemblies
                                             ? Channel.empty()
                                             : Channel.fromSamplesheet('synteny_xref_assemblies')
+
+    ch_xref_assembly_validated              = ch_xref_assembly
+                                            | map { row -> row[0] }
                                             | collect
-                                            | flatMap { validateXrefAssemblies(it) }
-                                            | buffer(size: synteny_xref_assemblies_fields)
+                                            | map { tags -> validateXrefAssemblies( tags ) }
+                                            | combine ( ch_xref_assembly.map { row -> [ row ] } )
+                                            | map { result, row -> row }
                                             | map { tag, fa, labels ->
                                                 [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ]
                                             }
@@ -117,9 +124,9 @@ workflow PIPELINE_INITIALISATION {
     ch_summary_params_as_json               = Channel.of ( jsonifySummaryParams ( summary_params ) )
 
     emit:
-    input                                   = ch_input
+    input                                   = ch_input_validated
     hic_reads                               = ch_hic_reads
-    xref_assembly                           = ch_xref_assembly
+    xref_assembly                           = ch_xref_assembly_validated
     params_as_json                          = ch_params_as_json
     summary_params_as_json                  = ch_summary_params_as_json
     versions                                = ch_versions
@@ -210,9 +217,7 @@ def validateInputParameters() {
     }
 }
 
-def validateInput(input) {
-    def inputFields = 5
-    def assemblyTags = input[(0..input.size()-1).step(inputFields)]
+def validateInputTags(assemblyTags) {
 
     def tagCounts = [:]
     assemblyTags.each { tag ->
@@ -224,12 +229,10 @@ def validateInput(input) {
         error("Please check input assemblysheet -> Multiple assemblies have the same tags!: ${repeatedTags}")
     }
 
-    return input
+    return true
 }
 
-def validateXrefAssemblies(xref) {
-    def xrefFields = 3
-    def xrefTags = xref[(0..xref.size()-1).step(xrefFields)]
+def validateXrefAssemblies(xrefTags) {
 
     def tagCounts = [:]
     xrefTags.each { tag ->
@@ -241,7 +244,7 @@ def validateXrefAssemblies(xref) {
         error("Please check synteny_xref_assemblies -> Multiple xref assemblies have the same tags!: ${repeatedTags}")
     }
 
-    return xref
+    return true
 }
 
 def jsonifyParams(params) {
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 7a6e7243..fea4a1e9 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -45,7 +45,10 @@ workflow ASSEMBLYQC {
 
     // Input channels
     ch_target_assemby_branch                = ch_input
-                                            | map { tag, fasta, gff, mono_ids, labels ->
+                                            | map { input_data ->
+                                                def tag     = input_data[0]
+                                                def fasta   = input_data[1]
+
                                                 [ [ id: tag ], file(fasta, checkIfExists: true) ]
                                             }
                                             | branch { meta, fasta ->
@@ -54,7 +57,10 @@ workflow ASSEMBLYQC {
                                             }
 
     ch_assemby_gff3_branch                  = ch_input
-                                            | map { tag, fasta, gff, mono_ids, labels ->
+                                            | map { input_data ->
+                                                def tag     = input_data[0]
+                                                def gff     = input_data[2]
+
                                                 gff
                                                 ? [ [ id: tag ], file(gff, checkIfExists: true) ]
                                                 : null
@@ -65,14 +71,20 @@ workflow ASSEMBLYQC {
                                             }
 
     ch_mono_ids                             = ch_input
-                                            | map { tag, fasta, gff, mono_ids, labels ->
+                                            | map { input_data ->
+                                                def tag     = input_data[0]
+                                                def mono_ids= input_data[3]
+
                                                 mono_ids
                                                 ? [ [ id: tag ], file(mono_ids, checkIfExists: true) ]
                                                 : null
                                             }
 
     ch_synteny_labels                       = ch_input
-                                            | map { tag, fasta, gff, mono_ids, labels ->
+                                            | map { input_data ->
+                                                def tag     = input_data[0]
+                                                def labels  = input_data[4]
+
                                                 labels
                                                 ? [ [ id: tag ], file(labels, checkIfExists: true) ]
                                                 : (

From 29b76a5ef4bc60371cab83f3e8dc8c3d7ce54a3f Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 8 May 2024 15:40:08 +1200
Subject: [PATCH 34/76] Now using FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS
 for SRA downloads

---
 CHANGELOG.md                                  |  1 +
 assets/schema_input.json                      | 12 +--
 conf/modules.config                           |  4 +
 local_assemblyqc                              |  3 +-
 modules.json                                  | 20 ++++
 .../sratoolsncbisettings/environment.yml      |  7 ++
 .../custom/sratoolsncbisettings/main.nf       | 23 +++++
 .../custom/sratoolsncbisettings/meta.yml      | 28 ++++++
 .../templates/detect_ncbi_settings.sh         | 45 +++++++++
 .../sratoolsncbisettings/tests/main.nf.test   | 42 ++++++++
 .../tests/main.nf.test.snap                   | 14 +++
 .../tests/nextflow.config                     | 14 +++
 .../sratoolsncbisettings/tests/tags.yml       |  2 +
 .../sratools/fasterqdump/environment.yml      |  8 ++
 modules/nf-core/sratools/fasterqdump/main.nf  | 55 +++++++++++
 modules/nf-core/sratools/fasterqdump/meta.yml | 53 ++++++++++
 .../sratools/fasterqdump/tests/main.nf.test   | 73 ++++++++++++++
 .../fasterqdump/tests/main.nf.test.snap       | 78 +++++++++++++++
 .../fasterqdump/tests/nextflow.config         |  5 +
 .../sratools/fasterqdump/tests/tags.yml       |  2 +
 .../nf-core/sratools/prefetch/environment.yml |  8 ++
 modules/nf-core/sratools/prefetch/main.nf     | 35 +++++++
 modules/nf-core/sratools/prefetch/meta.yml    | 56 +++++++++++
 .../prefetch/templates/retry_with_backoff.sh  | 65 +++++++++++++
 .../sratools/prefetch/tests/main.nf.test      | 49 ++++++++++
 .../sratools/prefetch/tests/main.nf.test.snap | 80 +++++++++++++++
 .../nf-core/sratools/prefetch/tests/tags.yml  |  2 +
 .../utils_nfcore_assemblyqc_pipeline/main.nf  |  8 +-
 .../main.nf                                   | 39 ++++++++
 .../meta.yml                                  | 52 ++++++++++
 .../tests/main.nf.test                        | 43 ++++++++
 .../tests/main.nf.test.snap                   | 97 +++++++++++++++++++
 .../tests/tags.yml                            |  2 +
 workflows/assemblyqc.nf                       | 20 +++-
 34 files changed, 1033 insertions(+), 12 deletions(-)
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/environment.yml
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/main.nf
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/meta.yml
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/templates/detect_ncbi_settings.sh
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/tests/nextflow.config
 create mode 100644 modules/nf-core/custom/sratoolsncbisettings/tests/tags.yml
 create mode 100644 modules/nf-core/sratools/fasterqdump/environment.yml
 create mode 100644 modules/nf-core/sratools/fasterqdump/main.nf
 create mode 100644 modules/nf-core/sratools/fasterqdump/meta.yml
 create mode 100644 modules/nf-core/sratools/fasterqdump/tests/main.nf.test
 create mode 100644 modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/sratools/fasterqdump/tests/nextflow.config
 create mode 100644 modules/nf-core/sratools/fasterqdump/tests/tags.yml
 create mode 100644 modules/nf-core/sratools/prefetch/environment.yml
 create mode 100644 modules/nf-core/sratools/prefetch/main.nf
 create mode 100644 modules/nf-core/sratools/prefetch/meta.yml
 create mode 100755 modules/nf-core/sratools/prefetch/templates/retry_with_backoff.sh
 create mode 100644 modules/nf-core/sratools/prefetch/tests/main.nf.test
 create mode 100644 modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/sratools/prefetch/tests/tags.yml
 create mode 100644 subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main.nf
 create mode 100644 subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/meta.yml
 create mode 100644 subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/tags.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7541902a..40e49026 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
 9. Updated `fcs-adaptor` to 0.5.0 [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
 10. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
+11. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
 
 ### `Fixed`
 
diff --git a/assets/schema_input.json b/assets/schema_input.json
index cd19ce8b..759ea3af 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -49,11 +49,11 @@
                 ]
             },
             "reads_1": {
-                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "errorMessage": "reads_1 should be a SRA ID or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
-                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                        "pattern": "^SR\\w+$|^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
                     },
                     {
                         "type": "string",
@@ -76,11 +76,11 @@
                 "dependentRequired": ["reads_1"]
             },
             "maternal_reads_1": {
-                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "errorMessage": "maternal_reads_1 should be a SRA ID or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
-                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                        "pattern": "^SR\\w+$|^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
                     },
                     {
                         "type": "string",
@@ -104,11 +104,11 @@
                 "dependentRequired": ["maternal_reads_1"]
             },
             "paternal_reads_1": {
-                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "errorMessage": "paternal_reads_1 should be a SRA ID or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
-                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                        "pattern": "^SR\\w+$|^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
                     },
                     {
                         "type": "string",
diff --git a/conf/modules.config b/conf/modules.config
index c532b809..dd8cc10c 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -215,6 +215,10 @@ process {
         ]
     }
 
+    withName: '.*:DOWNLOAD_HIC:SRATOOLS_FASTERQDUMP' {
+        ext.prefix = { "${meta.id}.sra.fastq" }
+    }
+
     withName: FASTQC_RAW {
         publishDir = [
             path: { "${params.outdir}/hic/fastqc_raw" },
diff --git a/local_assemblyqc b/local_assemblyqc
index f8dd29a2..858acea8 100755
--- a/local_assemblyqc
+++ b/local_assemblyqc
@@ -23,5 +23,4 @@ nextflow run \
     --ncbi_fcs_gx_db_path ../dbs/gxdb/test \
     --busco_download_path ../dbs/busco \
     --kraken2_skip false \
-    --kraken2_db_path ../dbs/kraken2db/k2_minusb \
-    --hic "$(realpath ../test-data/assemblyqc/hic)/SRR8238190_{1,2}.fastq.gz"
+    --kraken2_db_path ../dbs/kraken2db/k2_minusb
diff --git a/modules.json b/modules.json
index f8f52b45..d387fde5 100644
--- a/modules.json
+++ b/modules.json
@@ -100,6 +100,11 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
+                    "custom/sratoolsncbisettings": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools"]
+                    },
                     "fastavalidator": {
                         "branch": "master",
                         "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
@@ -130,6 +135,16 @@
                         "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d",
                         "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
+                    "sratools/fasterqdump": {
+                        "branch": "master",
+                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"]
+                    },
+                    "sratools/prefetch": {
+                        "branch": "master",
+                        "git_sha": "1fc29f92e439d5631fdf34b8ac4687297d70f5ec",
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"]
+                    },
                     "tidk/explore": {
                         "branch": "master",
                         "git_sha": "fad335b31b32d78f89a8340373d3ccfc0f2b18b5",
@@ -159,6 +174,11 @@
                         "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5",
                         "installed_by": ["subworkflows"]
                     },
+                    "fastq_download_prefetch_fasterqdump_sratools": {
+                        "branch": "master",
+                        "git_sha": "1fc29f92e439d5631fdf34b8ac4687297d70f5ec",
+                        "installed_by": ["subworkflows"]
+                    },
                     "fastq_trim_fastp_fastqc": {
                         "branch": "master",
                         "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
diff --git a/modules/nf-core/custom/sratoolsncbisettings/environment.yml b/modules/nf-core/custom/sratoolsncbisettings/environment.yml
new file mode 100644
index 00000000..44a1b008
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/environment.yml
@@ -0,0 +1,7 @@
+name: custom_sratoolsncbisettings
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::sra-tools=3.0.8
diff --git a/modules/nf-core/custom/sratoolsncbisettings/main.nf b/modules/nf-core/custom/sratoolsncbisettings/main.nf
new file mode 100644
index 00000000..577117ed
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/main.nf
@@ -0,0 +1,23 @@
+process CUSTOM_SRATOOLSNCBISETTINGS {
+    tag 'ncbi-settings'
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sra-tools:3.0.8--h9f5acd7_0' :
+        'biocontainers/sra-tools:3.0.8--h9f5acd7_0' }"
+
+    input:
+    val ids
+
+    output:
+    path('*.mkfg')     , emit: ncbi_settings
+    path 'versions.yml', emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    shell:
+    config = "/LIBS/GUID = \"${UUID.randomUUID().toString()}\"\\n/libs/cloud/report_instance_identity = \"true\"\\n"
+    template 'detect_ncbi_settings.sh'
+}
diff --git a/modules/nf-core/custom/sratoolsncbisettings/meta.yml b/modules/nf-core/custom/sratoolsncbisettings/meta.yml
new file mode 100644
index 00000000..46a6cd32
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/meta.yml
@@ -0,0 +1,28 @@
+name: "custom_sratoolsncbisettings"
+description: Test for the presence of suitable NCBI settings or create them on the fly.
+keywords:
+  - NCBI
+  - settings
+  - sra-tools
+  - prefetch
+  - fasterq-dump
+tools:
+  - "sratools":
+      description: "SRA Toolkit and SDK from NCBI"
+      homepage: https://github.com/ncbi/sra-tools
+      documentation: https://github.com/ncbi/sra-tools/wiki
+      tool_dev_url: https://github.com/ncbi/sra-tools
+      licence: ["Public Domain"]
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - ncbi_settings:
+      type: file
+      description: An NCBI user settings file.
+      pattern: "*.mkfg"
+authors:
+  - "@Midnighter"
+maintainers:
+  - "@Midnighter"
diff --git a/modules/nf-core/custom/sratoolsncbisettings/templates/detect_ncbi_settings.sh b/modules/nf-core/custom/sratoolsncbisettings/templates/detect_ncbi_settings.sh
new file mode 100644
index 00000000..cfe3a324
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/templates/detect_ncbi_settings.sh
@@ -0,0 +1,45 @@
+#!/usr/bin/env bash
+
+set -u
+
+
+# Get the expected NCBI settings path and define the environment variable
+# `NCBI_SETTINGS`.
+eval "$(vdb-config -o n NCBI_SETTINGS | sed 's/[" ]//g')"
+
+# If the user settings do not exist yet, create a file suitable for `prefetch`
+# and `fasterq-dump`. If an existing settings file does not contain the required
+# values, error out with a helpful message.
+if [[ ! -f "${NCBI_SETTINGS}" ]]; then
+    printf '!{config}' > 'user-settings.mkfg'
+else
+    prefetch --help &> /dev/null
+    if [[ $? = 78 ]]; then
+        echo "You have an existing vdb-config at '${NCBI_SETTINGS}' but it is"\
+        "missing the required entries for /LIBS/GUID and"\
+        "/libs/cloud/report_instance_identity."\
+        "Feel free to add the following to your settings file:" >&2
+        echo "$(printf '!{config}')" >&2
+        exit 1
+    fi
+    fasterq-dump --help &> /dev/null
+    if [[ $? = 78 ]]; then
+        echo "You have an existing vdb-config at '${NCBI_SETTINGS}' but it is"\
+        "missing the required entries for /LIBS/GUID and"\
+        "/libs/cloud/report_instance_identity."\
+        "Feel free to add the following to your settings file:" >&2
+        echo "$(printf '!{config}')" >&2
+        exit 1
+    fi
+    if [[ "${NCBI_SETTINGS}" != *.mkfg ]]; then
+        echo "The detected settings '${NCBI_SETTINGS}' do not have the required"\
+        "file extension '.mkfg'." >&2
+        exit 1
+    fi
+    cp "${NCBI_SETTINGS}" ./
+fi
+
+cat <<-END_VERSIONS > versions.yml
+"!{task.process}":
+    sratools: $(vdb-config --version 2>&1 | grep -Eo '[0-9.]+')
+END_VERSIONS
diff --git a/modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test b/modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test
new file mode 100644
index 00000000..b8b95320
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test
@@ -0,0 +1,42 @@
+nextflow_process {
+
+    name "Test Process CUSTOM_SRATOOLSNCBISETTINGS"
+    script "../main.nf"
+    process "CUSTOM_SRATOOLSNCBISETTINGS"
+    config "modules/nf-core/custom/sratoolsncbisettings/tests/nextflow.config"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "custom"
+    tag "custom/sratoolsncbisettings"
+
+    test("Should run without failures") {
+
+        when {
+            params {
+                settings_path = '/tmp/.ncbi'
+                settings_file  = "${params.settings_path}/user-settings.mkfg"
+            }
+
+            process {
+                """
+                input[0] = ["SRX6725035"]
+                file(params.settings_path).mkdirs()
+                def settings = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                settings.copyTo(params.settings_file)
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(
+                process.out.versions
+            ).match()
+
+            with(process.out.ncbi_settings) {
+                assert path(get(0)).readLines().any { it.contains('/LIBS/GUID') }
+                assert path(get(0)).readLines().any { it.contains('/libs/cloud/report_instance_identity') }
+            }
+        }
+    }
+}
diff --git a/modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test.snap b/modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test.snap
new file mode 100644
index 00000000..5e314f0b
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/tests/main.nf.test.snap
@@ -0,0 +1,14 @@
+{
+    "Should run without failures": {
+        "content": [
+            [
+                "versions.yml:md5,3d6ee88cce1ee517e198633f062589a8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T11:47:15.824443"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/custom/sratoolsncbisettings/tests/nextflow.config b/modules/nf-core/custom/sratoolsncbisettings/tests/nextflow.config
new file mode 100644
index 00000000..df5def04
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/tests/nextflow.config
@@ -0,0 +1,14 @@
+params.settings_path = '/tmp/.ncbi'
+params.settings_file = "${params.settings_path}/user-settings.mkfg"
+
+env.NCBI_SETTINGS = params.settings_file
+
+process {
+    withName: CUSTOM_SRATOOLSNCBISETTINGS {
+        containerOptions = {
+            (workflow.containerEngine == 'singularity') ?
+            "-B ${params.settings_path}:${params.settings_path}" :
+            "-v ${params.settings_path}:${params.settings_path}"
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/custom/sratoolsncbisettings/tests/tags.yml b/modules/nf-core/custom/sratoolsncbisettings/tests/tags.yml
new file mode 100644
index 00000000..fb4a08a7
--- /dev/null
+++ b/modules/nf-core/custom/sratoolsncbisettings/tests/tags.yml
@@ -0,0 +1,2 @@
+custom/sratoolsncbisettings:
+  - modules/nf-core/custom/sratoolsncbisettings/**
diff --git a/modules/nf-core/sratools/fasterqdump/environment.yml b/modules/nf-core/sratools/fasterqdump/environment.yml
new file mode 100644
index 00000000..4011b691
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/environment.yml
@@ -0,0 +1,8 @@
+name: sratools_fasterqdump
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::sra-tools=3.0.8
+  - conda-forge::pigz=2.6
diff --git a/modules/nf-core/sratools/fasterqdump/main.nf b/modules/nf-core/sratools/fasterqdump/main.nf
new file mode 100644
index 00000000..d4ddf268
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/main.nf
@@ -0,0 +1,55 @@
+process SRATOOLS_FASTERQDUMP {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-5f89fe0cd045cb1d615630b9261a1d17943a9b6a:2f4a4c900edd6801ff0068c2b3048b4459d119eb-0' :
+        'biocontainers/mulled-v2-5f89fe0cd045cb1d615630b9261a1d17943a9b6a:2f4a4c900edd6801ff0068c2b3048b4459d119eb-0' }"
+
+    input:
+    tuple val(meta), path(sra)
+    path ncbi_settings
+    path certificate
+
+    output:
+    tuple val(meta), path('*.fastq.gz'), emit: reads
+    path "versions.yml"                , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def outfile = meta.single_end ? "${prefix}.fastq" : prefix
+    def key_file = ''
+    if (certificate.toString().endsWith('.jwt')) {
+        key_file += " --perm ${certificate}"
+    } else if (certificate.toString().endsWith('.ngc')) {
+        key_file += " --ngc ${certificate}"
+    }
+    """
+    export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
+
+    fasterq-dump \\
+        $args \\
+        --threads $task.cpus \\
+        --outfile $outfile \\
+        ${key_file} \\
+        ${sra}
+
+    pigz \\
+        $args2 \\
+        --no-name \\
+        --processes $task.cpus \\
+        *.fastq
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sratools: \$(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/sratools/fasterqdump/meta.yml b/modules/nf-core/sratools/fasterqdump/meta.yml
new file mode 100644
index 00000000..b5e0175a
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/meta.yml
@@ -0,0 +1,53 @@
+name: sratools_fasterqdump
+description: Extract sequencing reads in FASTQ format from a given NCBI Sequence Read Archive (SRA).
+keywords:
+  - sequencing
+  - FASTQ
+  - dump
+tools:
+  - sratools:
+      description: SRA Toolkit and SDK from NCBI
+      homepage: https://github.com/ncbi/sra-tools
+      documentation: https://github.com/ncbi/sra-tools/wiki
+      tool_dev_url: https://github.com/ncbi/sra-tools
+      licence: ["Public Domain"]
+input:
+  - meta:
+      type: map
+      description: >
+        Groovy Map containing sample information e.g. [ id:'test', single_end:false ]
+
+  - sra:
+      type: directory
+      description: Directory containing ETL data for the given SRA.
+      pattern: "*/*.sra"
+  - ncbi_settings:
+      type: file
+      description: >
+        An NCBI user settings file.
+
+      pattern: "*.mkfg"
+  - certificate:
+      type: file
+      description: >
+        Path to a JWT cart file used to access protected dbGAP data on SRA using the sra-toolkit
+
+      pattern: "*.cart"
+output:
+  - meta:
+      type: map
+      description: >
+        Groovy Map containing sample information e.g. [ id:'test', single_end:false ]
+
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - reads:
+      type: file
+      description: Extracted FASTQ file or files if the sequencing reads are paired-end.
+      pattern: "*.fastq.gz"
+authors:
+  - "@Midnighter"
+maintainers:
+  - "@Midnighter"
diff --git a/modules/nf-core/sratools/fasterqdump/tests/main.nf.test b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test
new file mode 100644
index 00000000..cb81496f
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test
@@ -0,0 +1,73 @@
+nextflow_process {
+    name "Test Process SRATOOLS_FASTERQDUMP"
+    script "../main.nf"
+    config "./nextflow.config"
+    process "SRATOOLS_FASTERQDUMP"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "untar"
+    tag "sratools"
+    tag "sratools/fasterqdump"
+
+    test("Single-end") {
+
+        setup {
+            run("UNTAR") {
+                script "modules/nf-core/untar/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR13255544.tar.gz', checkIfExists: true) ])
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = UNTAR.out.untar.map{ meta, files -> [ [ id:'test_single_end', single_end:true ], files]}
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("Paired-end") {
+
+        setup {
+            run("UNTAR") {
+                script "modules/nf-core/untar/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR11140744.tar.gz', checkIfExists: true) ])
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = UNTAR.out.untar.map{ meta, files -> [ [ id:'test_paired_end', single_end:false ], files]}
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
new file mode 100644
index 00000000..14a6429d
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
@@ -0,0 +1,78 @@
+{
+    "Single-end": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_single_end",
+                            "single_end": true
+                        },
+                        "test_single_end.fastq.gz:md5,674d78c1cc3c1308d6d39d6369a42887"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test_single_end",
+                            "single_end": true
+                        },
+                        "test_single_end.fastq.gz:md5,674d78c1cc3c1308d6d39d6369a42887"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-28T15:25:52.837288"
+    },
+    "Paired-end": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_paired_end",
+                            "single_end": false
+                        },
+                        [
+                            "test_paired_end_1.fastq.gz:md5,8573015c91d099b6e30789f8bab2f43c",
+                            "test_paired_end_2.fastq.gz:md5,37e6f719a022dc3c9994c80fbc20c311"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test_paired_end",
+                            "single_end": false
+                        },
+                        [
+                            "test_paired_end_1.fastq.gz:md5,8573015c91d099b6e30789f8bab2f43c",
+                            "test_paired_end_2.fastq.gz:md5,37e6f719a022dc3c9994c80fbc20c311"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-28T15:26:42.466223"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/sratools/fasterqdump/tests/nextflow.config b/modules/nf-core/sratools/fasterqdump/tests/nextflow.config
new file mode 100644
index 00000000..23e4100b
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: SRATOOLS_FASTERQDUMP {
+        ext.args = ''
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/sratools/fasterqdump/tests/tags.yml b/modules/nf-core/sratools/fasterqdump/tests/tags.yml
new file mode 100644
index 00000000..5d1ddcb3
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/tests/tags.yml
@@ -0,0 +1,2 @@
+sratools/fasterqdump:
+  - modules/nf-core/sratools/fasterqdump/**
diff --git a/modules/nf-core/sratools/prefetch/environment.yml b/modules/nf-core/sratools/prefetch/environment.yml
new file mode 100644
index 00000000..0abad336
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/environment.yml
@@ -0,0 +1,8 @@
+name: sratools_prefetch
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::sra-tools=3.1.0
+  - conda-forge::curl=8.5.0
diff --git a/modules/nf-core/sratools/prefetch/main.nf b/modules/nf-core/sratools/prefetch/main.nf
new file mode 100644
index 00000000..170f1753
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/main.nf
@@ -0,0 +1,35 @@
+process SRATOOLS_PREFETCH {
+    tag "$id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sra-tools:3.1.0--h9f5acd7_0' :
+        'biocontainers/sra-tools:3.1.0--h9f5acd7_0' }"
+
+    input:
+    tuple val(meta), val(id)
+    path ncbi_settings
+    path certificate
+
+    output:
+    tuple val(meta), path(id), emit: sra
+    path 'versions.yml'      , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    shell:
+    args = task.ext.args ?: ''
+    args2 = task.ext.args2 ?: '5 1 100'  // <num retries> <base delay in seconds> <max delay in seconds>
+    if (certificate) {
+        if (certificate.toString().endsWith('.jwt')) {
+            args += " --perm ${certificate}"
+        }
+        else if (certificate.toString().endsWith('.ngc')) {
+            args += " --ngc ${certificate}"
+        }
+    }
+
+    template 'retry_with_backoff.sh'
+}
diff --git a/modules/nf-core/sratools/prefetch/meta.yml b/modules/nf-core/sratools/prefetch/meta.yml
new file mode 100644
index 00000000..ff54229f
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/meta.yml
@@ -0,0 +1,56 @@
+name: sratools_prefetch
+description: Download sequencing data from the NCBI Sequence Read Archive (SRA).
+keywords:
+  - sequencing
+  - fastq
+  - prefetch
+tools:
+  - sratools:
+      description: SRA Toolkit and SDK from NCBI
+      homepage: https://github.com/ncbi/sra-tools
+      documentation: https://github.com/ncbi/sra-tools/wiki
+      tool_dev_url: https://github.com/ncbi/sra-tools
+      licence: ["Public Domain"]
+input:
+  - meta:
+      type: map
+      description: >
+        Groovy Map containing sample information e.g. [ id:'test', single_end:false ]
+
+  - id:
+      type: string
+      description: >
+        A string denoting an SRA id.
+
+  - ncbi_settings:
+      type: file
+      description: >
+        An NCBI user settings file.
+
+      pattern: "*.mkfg"
+  - certificate:
+      type: file
+      description: >
+        Path to a JWT cart file used to access protected dbGAP data on SRA using the sra-toolkit
+
+      pattern: "*.cart"
+output:
+  - meta:
+      type: map
+      description: >
+        Groovy Map containing sample information e.g. [ id:'test', single_end:false ]
+
+  - sra:
+      type: directory
+      description: >
+        Directory containing the ETL data for the given SRA id.
+
+      pattern: "*/*.sra"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@Midnighter"
+maintainers:
+  - "@Midnighter"
diff --git a/modules/nf-core/sratools/prefetch/templates/retry_with_backoff.sh b/modules/nf-core/sratools/prefetch/templates/retry_with_backoff.sh
new file mode 100755
index 00000000..bfee6070
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/templates/retry_with_backoff.sh
@@ -0,0 +1,65 @@
+#!/usr/bin/env bash
+
+set -u
+
+retry_with_backoff() {
+    local max_attempts=${1}
+    local delay=${2}
+    local max_time=${3}
+    local attempt=1
+    local output=
+    local status=
+
+    # Remove the first three arguments to this function in order to access
+    # the 'real' command with `${@}`.
+    shift 3
+
+    while [ ${attempt} -le ${max_attempts} ]; do
+        output=$("${@}")
+        status=${?}
+
+        if [ ${status} -eq 0 ]; then
+            break
+        fi
+
+        if [ ${attempt} -lt ${max_attempts} ]; then
+            echo "Failed attempt ${attempt} of ${max_attempts}. Retrying in ${delay} s." >&2
+            sleep ${delay}
+        elif [ ${attempt} -eq ${max_attempts} ]; then
+            echo "Failed after ${attempt} attempts." >&2
+            return ${status}
+        fi
+
+        attempt=$(( ${attempt} + 1 ))
+        delay=$(( ${delay} * 2 ))
+        if [ ${delay} -ge ${max_time} ]; then
+            delay=${max_time}
+        fi
+    done
+
+    echo "${output}"
+}
+
+export NCBI_SETTINGS="$PWD/!{ncbi_settings}"
+
+retry_with_backoff !{args2} \
+    prefetch \
+    !{args} \
+    !{id}
+
+# check file integrity using vdb-validate or (when archive contains no checksums) md5sum
+vdb-validate !{id} > vdb-validate_result.txt 2>&1 || exit 1
+if grep -q "checksums missing" vdb-validate_result.txt; then
+    VALID_MD5SUMS=$(curl --silent --fail --location --retry 3 --retry-delay 60 'https://locate.ncbi.nlm.nih.gov/sdl/2/retrieve?filetype=run&acc=!{id}')
+    LOCAL_MD5SUMS=$(md5sum !{id}/* | cut -f1 -d' ')
+    if ! grep -q -F -f <(echo "$LOCAL_MD5SUMS") <(echo "$VALID_MD5SUMS"); then
+        echo "MD5 sum check failed" 1>&2
+        exit 1
+    fi
+fi
+
+cat <<-END_VERSIONS > versions.yml
+"!{task.process}":
+    sratools: $(prefetch --version 2>&1 | grep -Eo '[0-9.]+')
+    curl: $(curl --version | head -n 1 | sed 's/^curl //; s/ .*$//')
+END_VERSIONS
diff --git a/modules/nf-core/sratools/prefetch/tests/main.nf.test b/modules/nf-core/sratools/prefetch/tests/main.nf.test
new file mode 100644
index 00000000..f59de7cb
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/tests/main.nf.test
@@ -0,0 +1,49 @@
+nextflow_process {
+    name "Test Process SRATOOLS_PREFETCH"
+    script "../main.nf"
+    process "SRATOOLS_PREFETCH"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "sratools"
+    tag "sratools/prefetch"
+
+    test("sratools/prefetch") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([ [ id:'test', single_end:false ], 'DRR000774' ])
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sratools/prefetch with sralite") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([ [ id:'test', single_end:false ], 'SRR1170046' ])
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap b/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
new file mode 100644
index 00000000..67795ebb
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
@@ -0,0 +1,80 @@
+{
+    "sratools/prefetch with sralite": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "SRR1170046.sralite:md5,7acfce556ca0951aff49d780899c105b"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
+                ],
+                "sra": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "SRR1170046.sralite:md5,7acfce556ca0951aff49d780899c105b"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T11:49:02.309737"
+    },
+    "sratools/prefetch": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "DRR000774.sra:md5,7647dba20c89c0e3d7ad13842f060eb0"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
+                ],
+                "sra": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "DRR000774.sra:md5,7647dba20c89c0e3d7ad13842f060eb0"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T11:48:37.428307"
+    }
+}
diff --git a/modules/nf-core/sratools/prefetch/tests/tags.yml b/modules/nf-core/sratools/prefetch/tests/tags.yml
new file mode 100644
index 00000000..52110bfd
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/tests/tags.yml
@@ -0,0 +1,2 @@
+sratools/prefetch:
+  - modules/nf-core/sratools/prefetch/**
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 978ad199..52d742bc 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -99,10 +99,12 @@ workflow PIPELINE_INITIALISATION {
                                             : (
                                                 "$params.hic".find(/.*[\/].*\.(fastq|fq)\.gz/)
                                                 ? Channel.fromFilePairs(params.hic, checkIfExists: true)
-                                                : Channel.fromSRA(params.hic)
+                                                : Channel.of( [ params.hic, 'is_sra' ] )
                                             )
-                                            | map{ sample, fq ->
-                                                [ [ id: sample, single_end: false ], fq ]
+                                            | map { sample, fq ->
+                                                "$fq" != 'is_sra'
+                                                ? [ [ id: sample, single_end: false, is_sra: false ], fq ]
+                                                : [ [ id: sample, single_end: false, is_sra: true ], sample ]
                                             }
 
     ch_xref_assembly                        = params.synteny_skip || ! params.synteny_xref_assemblies
diff --git a/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main.nf b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main.nf
new file mode 100644
index 00000000..fbeacf4a
--- /dev/null
+++ b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main.nf
@@ -0,0 +1,39 @@
+include { CUSTOM_SRATOOLSNCBISETTINGS } from '../../../modules/nf-core/custom/sratoolsncbisettings/main'
+include { SRATOOLS_PREFETCH           } from '../../../modules/nf-core/sratools/prefetch/main'
+include { SRATOOLS_FASTERQDUMP        } from '../../../modules/nf-core/sratools/fasterqdump/main'
+
+//
+// Download FASTQ sequencing reads from the NCBI's Sequence Read Archive (SRA).
+//
+workflow FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS {
+    take:
+    ch_sra_ids   // channel: [ val(meta), val(id) ]
+    ch_dbgap_key // channel: [ path(dbgap_key) ]
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    //
+    // Detect existing NCBI user settings or create new ones.
+    //
+    CUSTOM_SRATOOLSNCBISETTINGS ( ch_sra_ids.collect() )
+    ch_ncbi_settings = CUSTOM_SRATOOLSNCBISETTINGS.out.ncbi_settings
+    ch_versions = ch_versions.mix(CUSTOM_SRATOOLSNCBISETTINGS.out.versions)
+
+    //
+    // Prefetch sequencing reads in SRA format.
+    //
+    SRATOOLS_PREFETCH ( ch_sra_ids, ch_ncbi_settings, ch_dbgap_key )
+    ch_versions = ch_versions.mix(SRATOOLS_PREFETCH.out.versions.first())
+
+    //
+    // Convert the SRA format into one or more compressed FASTQ files.
+    //
+    SRATOOLS_FASTERQDUMP ( SRATOOLS_PREFETCH.out.sra, ch_ncbi_settings, ch_dbgap_key )
+    ch_versions = ch_versions.mix(SRATOOLS_FASTERQDUMP.out.versions.first())
+
+    emit:
+    reads    = SRATOOLS_FASTERQDUMP.out.reads // channel: [ val(meta), [ reads ] ]
+    versions = ch_versions                    // channel: [ versions.yml ]
+}
diff --git a/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/meta.yml b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/meta.yml
new file mode 100644
index 00000000..1b968acc
--- /dev/null
+++ b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/meta.yml
@@ -0,0 +1,52 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: fastq_download_prefetch_fasterqdump_sratools
+description: Download FASTQ sequencing reads from the NCBI's Sequence Read Archive (SRA).
+keywords:
+  - SRA
+  - NCBI
+  - sequencing
+  - fastq
+  - prefetch
+  - fasterq-dump
+components:
+  - custom/sratoolsncbisettings
+  - sratools/prefetch
+  - sratools/fasterqdump
+input:
+  - meta:
+      type: map
+      description: >
+        Groovy Map containing sample information e.g. [ id:'test', single_end:false ]
+
+  - id:
+      type: string
+      description: >
+        SRA run identifier.
+
+  - certificate:
+      type: file
+      description: >
+        Path to a JWT cart file used to access protected dbGAP data on SRA using the sra-toolkit
+
+      pattern: "*.cart"
+# TODO Update when we decide on a standard for subworkflow docs
+output:
+  - meta:
+      type: map
+      description: >
+        Groovy Map containing sample information e.g. [ id:'test', single_end:false ]
+
+  - reads:
+      type: file
+      description: Extracted FASTQ file or files if the sequencing reads are paired-end.
+      pattern: "*.fastq.gz"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@Midnighter"
+  - "@drpatelh"
+maintainers:
+  - "@Midnighter"
+  - "@drpatelh"
diff --git a/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test
new file mode 100644
index 00000000..5f74ed20
--- /dev/null
+++ b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test
@@ -0,0 +1,43 @@
+nextflow_workflow {
+
+    name "Test workflow: fastq_download_prefetch_fasterqdump_sratools/main.nf"
+    script "../main.nf"
+    workflow "FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "custom/sratoolsncbisettings"
+    tag "sratools/prefetch"
+    tag "sratools/fasterqdump"
+    tag "subworkflows/fastq_download_prefetch_fasterqdump_sratools"
+
+    test("Parameters: default") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of(
+                    [[ id:'test_single_end', single_end:true  ], 'DRR000774'],
+                    [[ id:'test_paired_end', single_end:false ], 'SRR11140744']
+                )
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            def pelines1 = path(workflow.out.reads[0][1][0]).linesGzip
+            def pelines2 = path(workflow.out.reads[0][1][1]).linesGzip
+            def selines = path(workflow.out.reads[1][1]).linesGzip
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(pelines1[0..5]).match("test_pe_reads_1_lines") },
+                { assert snapshot(pelines1.size()).match("test_pe_reads_1_size") },
+                { assert snapshot(pelines2[0..5]).match("test_pe_reads_2_lines") },
+                { assert snapshot(pelines2.size()).match("test_pe_reads_2_size") },
+                { assert snapshot(selines[0..5]).match("test_se_reads_lines") },
+                { assert snapshot(selines.size()).match("test_se_reads_size") },
+                { assert snapshot(workflow.out.versions).match("versions") }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test.snap
new file mode 100644
index 00000000..1a34e0b8
--- /dev/null
+++ b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/main.nf.test.snap
@@ -0,0 +1,97 @@
+{
+    "test_se_reads_size": {
+        "content": [
+            19996
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:56.176292"
+    },
+    "test_pe_reads_2_lines": {
+        "content": [
+            [
+                "@SRR11140744.1 M01472:285:000000000-CYHNP:1:1101:12117:3295 length=251",
+                "ACAGGACACGAGTAACTCGTCTATCTTCTGCTGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAA",
+                "+SRR11140744.1 M01472:285:000000000-CYHNP:1:1101:12117:3295 length=251",
+                "ABAAAFBFFBDBGGGGGGGGGGHHHHHHHHHHCHGHGGGHHHGGHGGHGHGGGHFHHHHHHHHGGGGGHHHHHHHHHFHHHHGHHHGHGGGGGEFGDGHHGFGGGHHHHHGHHGGHHFHHHHGHHHHHHHHHHHHHHGFFGGHHHHHHGGHHGGHHHHHEGHHHHHHHGHHGHHFHHHHHGGGGGGGGGGGGAGGG9BEFFFFFFFFFFFFFFEEFFFFFFFA.FFFFFFFEFEFFFFFFF.BFFFFFFFB",
+                "@SRR11140744.2 M01472:285:000000000-CYHNP:1:1101:20752:3564 length=238",
+                "GTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACG"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:56.166207"
+    },
+    "test_pe_reads_2_size": {
+        "content": [
+            2011460
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:56.168869"
+    },
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,1a2218ff913fc33408bffccb081b5048",
+                "versions.yml:md5,53d6e983afde3a28add2ffc6b7eba4f3",
+                "versions.yml:md5,9c558ff624585a6eee82a19c8c0136db"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-28T15:19:18.755939"
+    },
+    "test_pe_reads_1_size": {
+        "content": [
+            2011460
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-28T15:19:18.677234"
+    },
+    "test_se_reads_lines": {
+        "content": [
+            [
+                "@DRR000774.1 1 length=421",
+                "ACGCAGGTGCCAGCAGCCGCGGTAATACGTAGGATCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGTGCGTAGGCGGCTTGTCAAGTCTCATGTGAAATCTCCCGGCTCAACTGGGAGGGTCATGGGAAACTGATGAGCTCGAGGGCAGTAGAGGGAAGCGGAATTCCGAGAGTAGTGGTGAAATGCGTAGATACTCGGAGGAACACCAGTGGCGAAAGCGGCTTCCTGGACTGTACCTGACGCTGAGGCACGAAAGCGTGGGGAGCAAACCGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGGATACTAGATATAGGGGGTATCGACCCTCTGTGTCGAAGCTAACGCATTAAGTATCCCGCCTGAGGAGTACGGCCGCAAGGCTAAAACTTAAGGAATTGACGGCTGCGT",
+                "+DRR000774.1 1 length=421",
+                "FFFFFFFFFFFIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:88FFF888???DBBBBB666F222ADDDFFF::;FFFFFFFFFFFFFFFFFFFFFFFFFFFF9:::FFFFCCCFFFFDDDFFFFF<<<FFFFFFFFFFFFFFFFFFFFFDDDFFFFFCC???FFCAADD::9FFFFD;;;FFFFFFFFFFFFFFFGA?==BA==2221<6@;<<@@551?ADDDDD:::CC?DBABBA5552219;;87<7988?====BDD:::?57555??=<4/0004044???ABDDDDDDDDB><<<8888886623//38><83238@B@@<;855557,,,,,,,0/0;;8:==DDDDDDDDD9:",
+                "@DRR000774.2 2 length=126",
+                "ACGCAGGTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTTTTCAAGTCAGGGGTGGAAATACCCGGGGCCGTCAACCCGACCG"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:56.171227"
+    },
+    "test_pe_reads_1_lines": {
+        "content": [
+            [
+                "@SRR11140744.1 M01472:285:000000000-CYHNP:1:1101:12117:3295 length=251",
+                "ACATAGGGCTGTTCAAGTTGAGGCAAAACGCCTTTTTCAACTTCTACTAAGCCACAAGTGCCATCTTTAAGATGTTGACGTGCCTCTGATAAGACCTCCTCCACGGAGTCTCCAAAGCCACGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTGAGTTGGACGTGTGTTTTCTCGTTGAAACCAGGGACAAGGCTCTCCATCTTACCTTTCGGTCACACCCGGACGAAACCTAGATGTGCTGATGA",
+                "+SRR11140744.1 M01472:285:000000000-CYHNP:1:1101:12117:3295 length=251",
+                "BCCCCFFFFFCFGGGGGGGGGGHGGHHHHGGGHGHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHGGGHHHHHGHHGHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHGHHHGGGGGHGHHGGGGGGGHHHHHHHHHHHGGHHHHHFHHHHHHHGGGHHHHHHHHHGGGHHHHHHHHGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFB",
+                "@SRR11140744.2 M01472:285:000000000-CYHNP:1:1101:20752:3564 length=238",
+                "CGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTGAGTTGGACGTGTGTTTTCTCGTTGAAACCAGGGACAAGGCTCTCCATCTTACCTTTCGGTCACACCCGGACGAAACCTAGATGTGCTGATGATCGGCTGCAACACGGACGAAACCGTAAGCAGCCTGCAGAAGATAGACGAGTTACTCGTGTCCTGTCAACGACAGTAATTAGTTATTAATTATACTGCGTGAGTGCAC"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:56.161354"
+    }
+}
diff --git a/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/tags.yml b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/tags.yml
new file mode 100644
index 00000000..03028c32
--- /dev/null
+++ b/subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/fastq_download_prefetch_fasterqdump_sratools:
+  - subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/**
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index fea4a1e9..892c142b 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -19,6 +19,7 @@ include { ASSEMBLATHON_STATS                } from '../modules/local/assemblatho
 include { FASTA_BUSCO_PLOT                  } from '../subworkflows/local/fasta_busco_plot'
 include { FASTA_LTRRETRIEVER_LAI            } from '../subworkflows/pfr/fasta_ltrretriever_lai/main'
 include { FASTA_KRAKEN2                     } from '../subworkflows/local/fasta_kraken2'
+include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_HIC } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
 include { FQ2HIC                            } from '../subworkflows/local/fq2hic'
 include { FASTA_SYNTENY                     } from '../subworkflows/local/fasta_synteny'
 include { CREATEREPORT                      } from '../modules/local/createreport'
@@ -356,6 +357,23 @@ workflow ASSEMBLYQC {
     ch_kraken2_plot                         = FASTA_KRAKEN2.out.plot
     ch_versions                             = ch_versions.mix(FASTA_KRAKEN2.out.versions)
 
+
+    // MODULE: FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_HIC
+    ch_hic_reads_branch                    = ch_hic_reads
+                                            | branch { meta, fq ->
+                                                sra: meta.is_sra
+                                                rest: ! meta.is_sra
+                                            }
+
+    DOWNLOAD_HIC(
+        ch_hic_reads_branch.sra,
+        []
+    )
+
+    ch_versions                             = ch_versions.mix(DOWNLOAD_HIC.out.versions)
+    ch_hic_read_files                       = DOWNLOAD_HIC.out.reads
+                                            | mix(ch_hic_reads_branch.rest)
+
     // SUBWORKFLOW: FQ2HIC
     ch_hic_input_assembly                   = ! params.hic
                                             ? Channel.empty()
@@ -363,7 +381,7 @@ workflow ASSEMBLYQC {
                                             | map { tag, fa -> [ [ id: tag ], fa ] }
 
     FQ2HIC(
-        ch_hic_reads,
+        ch_hic_read_files,
         ch_hic_input_assembly,
         params.hic_skip_fastp,
         params.hic_skip_fastqc

From 6cc2e845a04bc9cb02b0f6c5267740fc77b875aa Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 16 May 2024 13:40:11 +1200
Subject: [PATCH 35/76] Added MERQURYFK module

---
 CHANGELOG.md                                  |  14 +-
 CITATIONS.md                                  |   6 +
 README.md                                     |  16 +-
 assets/assemblysheetv2.csv                    |   2 +
 assets/schema_input.json                      |   6 +-
 bin/assemblyqc.py                             |   2 +
 .../parsers/merquryfk_parser.py               |  74 +++++++++
 bin/report_modules/templates/base.html        | 150 +++++++++++++-----
 .../templates/merquryfk/dropdown.html         |  10 ++
 .../templates/merquryfk/merquryfk.html        |  21 +++
 .../templates/merquryfk/report_contents.html  |  36 +++++
 conf/modules.config                           |  34 +++-
 conf/test_full.config                         |   4 +-
 docs/contributors.sh                          |   4 +-
 docs/images/spectra_cn.png                    | Bin 0 -> 90113 bytes
 docs/output.md                                |  17 ++
 docs/parameters.md                            |   7 +
 docs/usage.md                                 |   6 +
 main.nf                                       |   3 +
 modules.json                                  |  10 ++
 modules/local/createreport.nf                 |   1 +
 modules/nf-core/fastk/fastk/environment.yml   |   5 +
 modules/nf-core/fastk/fastk/main.nf           |  41 +++++
 modules/nf-core/fastk/fastk/meta.yml          |  49 ++++++
 .../merquryfk/merquryfk/environment.yml       |   5 +
 modules/nf-core/merquryfk/merquryfk/main.nf   |  58 +++++++
 modules/nf-core/merquryfk/merquryfk/meta.yml  | 112 +++++++++++++
 modules/pfr/bwa/index/environment.yml         |   2 +-
 modules/pfr/bwa/index/main.nf                 |   4 +-
 nextflow.config                               |   4 +
 nextflow_schema.json                          |  22 +++
 .../utils_nfcore_assemblyqc_pipeline/main.nf  | 101 ++++++++++++
 workflows/assemblyqc.nf                       |  87 +++++++++-
 33 files changed, 851 insertions(+), 62 deletions(-)
 create mode 100644 assets/assemblysheetv2.csv
 create mode 100644 bin/report_modules/parsers/merquryfk_parser.py
 create mode 100644 bin/report_modules/templates/merquryfk/dropdown.html
 create mode 100644 bin/report_modules/templates/merquryfk/merquryfk.html
 create mode 100644 bin/report_modules/templates/merquryfk/report_contents.html
 create mode 100644 docs/images/spectra_cn.png
 create mode 100644 modules/nf-core/fastk/fastk/environment.yml
 create mode 100644 modules/nf-core/fastk/fastk/main.nf
 create mode 100644 modules/nf-core/fastk/fastk/meta.yml
 create mode 100644 modules/nf-core/merquryfk/merquryfk/environment.yml
 create mode 100644 modules/nf-core/merquryfk/merquryfk/main.nf
 create mode 100644 modules/nf-core/merquryfk/merquryfk/meta.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 40e49026..96759b3c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## 2.0.0+dev - [06-May-2024]
+## 2.0.0+dev - [16-May-2024]
 
 ### `Added`
 
@@ -13,11 +13,13 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 4. Added a launcher script for local testing `local_assemblyqc`
 5. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
 6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
-7. Updated modules and sub-workflows
-8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
-9. Updated `fcs-adaptor` to 0.5.0 [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
-10. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
-11. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
+7. Updated modules and sub-workflows: `bwa/index`, `bwa/mem`, `cat/cat`, `custom/checkgff3fastacorrespondence`, `custom/restoregffids`, `custom/shortenfastaids`, `gt/gff3`, `gt/gff3validator`, `gt/stat`, `ltrfinder`, `ltrharvest`, `ltrretriever/lai`, `ltrretriever/ltrretriever`, `samblaster`, `fasta_ltrretriever_lai`, `fastq_bwa_mem_samblaster`, `gff3_validate`, `custom/sratoolsncbisettings`, `fastp`, `fastqc`, `untar`
+8. Locally bumped `bwa/index` version to `biocontainers/bwa:0.7.18--he4a0461_0`
+9. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
+10. Updated `fcs-adaptor` to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
+11. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
+12. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
+13. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
 
 ### `Fixed`
 
diff --git a/CITATIONS.md b/CITATIONS.md
index 63cbdd2b..dcd44eed 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -112,6 +112,12 @@
 
   > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: <https://doi.org/10.1371/journal.pcbi.1005944>. PMID: 29373581; PMCID: PMC5802927.
 
+- MERQURYFK, [Custom Permissive License](https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE)
+
+  > https://github.com/thegenemyers/MERQURY.FK
+  >
+  > Rhie, A., Walenz, B.P., Koren, S. et al. 2020. Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies. Genome Biol 21, 245. doi: <https://doi.org/10.1186/s13059-020-02134-9>
+
 ## Software packaging/containerisation tools
 
 - [Anaconda](https://anaconda.com)
diff --git a/README.md b/README.md
index 0b73d8ae..ceae669f 100644
--- a/README.md
+++ b/README.md
@@ -33,11 +33,11 @@ flowchart LR
   Run --> ASS_STATS[ASSEMBLATHON STATS]
   Run --> BUSCO
   Run --> TIDK
-  Run --> LTRRETRIEVER
-  LTRRETRIEVER --> LAI
+  Run --> LAI
   Run --> KRAKEN2
   Run --> HIC_CONTACT_MAP[HIC CONTACT MAP]
   Run --> SYNTENY
+  Run --> MERQURYFK
 
   ASS_STATS --> REPORT
   GFF_STATS --> REPORT
@@ -47,20 +47,22 @@ flowchart LR
   KRAKEN2 --> REPORT
   HIC_CONTACT_MAP --> REPORT
   SYNTENY --> REPORT
+  MERQURYFK --> REPORT
 ```
 
 - [FASTA VALIDATION](https://github.com/GallVp/fasta_validator)
-- [GFF3 VALIDATION](https://github.com/genometools/genometools)
+- [GFF3 VALIDATION](https://genometools.org/tools/gt_gff3validator.html)
 - [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics
-- [GENOMETOOLS GT STAT](https://github.com/genometools/genometools): Annotation statistics
+- [GENOMETOOLS GT STAT](https://genometools.org/tools/gt_stat.html): Annotation statistics
 - [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail
 - [NCBI FCS GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail
-- [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master): Gene-space completeness estimation
+- [BUSCO](https://gitlab.com/ezlab/busco): Gene-space completeness estimation
 - [TIDK](https://github.com/tolkit/telomeric-identifier): Telomere repeat identification
 - [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences
 - [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification
-- [HIC CONTACT MAP](https://github.com/igvteam/juicebox-web): Alignment and visualisation of HiC data
+- [HIC CONTACT MAP](https://github.com/igvteam/juicebox.js): Alignment and visualisation of HiC data
 - SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer), [CIRCOS](http://circos.ca/documentation/) and [PLOTLY](https://plotly.com)
+- [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK): k-mer based assembly evaluation
 
 ## Usage
 
@@ -126,8 +128,10 @@ The pipeline uses nf-core modules contributed by following authors.
 <a href="https://github.com/joseespinosa"><img src="https://github.com/joseespinosa.png" width="50" height="50"></a>
 <a href="https://github.com/kevinmenden"><img src="https://github.com/kevinmenden.png" width="50" height="50"></a>
 <a href="https://github.com/lescai"><img src="https://github.com/lescai.png" width="50" height="50"></a>
+<a href="https://github.com/mahesh-panchal"><img src="https://github.com/mahesh-panchal.png" width="50" height="50"></a>
 <a href="https://github.com/matthdsm"><img src="https://github.com/matthdsm.png" width="50" height="50"></a>
 <a href="https://github.com/maxulysse"><img src="https://github.com/maxulysse.png" width="50" height="50"></a>
+<a href="https://github.com/midnighter"><img src="https://github.com/midnighter.png" width="50" height="50"></a>
 
 ## Contributions and Support
 
diff --git a/assets/assemblysheetv2.csv b/assets/assemblysheetv2.csv
new file mode 100644
index 00000000..505e312c
--- /dev/null
+++ b/assets/assemblysheetv2.csv
@@ -0,0 +1,2 @@
+tag,fasta,gff3,monoploid_ids,synteny_labels,reads_1
+FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.seq.labels.tsv,SRR8238189
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 759ea3af..f415c25e 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -49,7 +49,7 @@
                 ]
             },
             "reads_1": {
-                "errorMessage": "reads_1 should be a SRA ID or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "errorMessage": "reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
@@ -76,7 +76,7 @@
                 "dependentRequired": ["reads_1"]
             },
             "maternal_reads_1": {
-                "errorMessage": "maternal_reads_1 should be a SRA ID or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "errorMessage": "maternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
@@ -104,7 +104,7 @@
                 "dependentRequired": ["maternal_reads_1"]
             },
             "paternal_reads_1": {
-                "errorMessage": "paternal_reads_1 should be a SRA ID or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "errorMessage": "paternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py
index 0a2e0b8c..73f7780c 100755
--- a/bin/assemblyqc.py
+++ b/bin/assemblyqc.py
@@ -25,6 +25,7 @@
 from report_modules.parsers.kraken2_parser import parse_kraken2_folder
 from report_modules.parsers.hic_parser import parse_hic_folder
 from report_modules.parsers.synteny_parser import parse_synteny_folder
+from report_modules.parsers.merquryfk_parser import parse_merquryfk_folder
 
 if __name__ == "__main__":
     params_dict, params_table = parse_params_json("params_json.json")
@@ -47,6 +48,7 @@
     data_from_tools = {**data_from_tools, **parse_kraken2_folder()}
     data_from_tools = {**data_from_tools, **parse_hic_folder()}
     data_from_tools = {**data_from_tools, **parse_synteny_folder()}
+    data_from_tools = {**data_from_tools, **parse_merquryfk_folder()}
 
     with open("software_versions.yml", "r") as f:
         versions_from_ch_versions = yaml.safe_load(f)
diff --git a/bin/report_modules/parsers/merquryfk_parser.py b/bin/report_modules/parsers/merquryfk_parser.py
new file mode 100644
index 00000000..22f8be27
--- /dev/null
+++ b/bin/report_modules/parsers/merquryfk_parser.py
@@ -0,0 +1,74 @@
+import base64
+import os
+import re
+
+from report_modules.parsers.parsing_commons import sort_list_of_results
+from tabulate import tabulate
+from pathlib import Path
+import pandas as pd
+
+
+def load_image_as_base64_str(file_name):
+    with open(file_name, "rb") as f:
+        binary_fc = f.read()
+
+    base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8")
+    return f"data:image/png+xml;base64,{base64_utf8_str}"
+
+
+def parse_merquryfk_folder(folder_name="merquryfk_outputs"):
+    dir = os.getcwdb().decode()
+    merqury_folder_path = Path(f"{dir}/{folder_name}")
+
+    if not os.path.exists(merqury_folder_path):
+        return {}
+
+    data = {"MERQURYFK": []}
+
+    completeness_stats_paths = [
+        item for item in merqury_folder_path.glob("*.completeness.stats")
+    ]
+
+    for completeness_stats_path in completeness_stats_paths:
+
+        data_id = os.path.basename(str(completeness_stats_path)).split(
+            ".completeness.stats"
+        )[0]
+        individual_id = data_id.split("-reads-")[0]
+        haplotypes = individual_id.split("-and-")
+
+        completeness_stats_table = pd.read_csv(completeness_stats_path, sep="\t")
+        qv_stats_table = pd.read_csv(f"{folder_name}/{data_id}.qv", sep="\t")
+
+        data["MERQURYFK"].append(
+            {
+                "individual_id": individual_id,
+                "completeness_stats_table": completeness_stats_table.to_dict("records"),
+                "completeness_stats_table_html": tabulate(
+                    completeness_stats_table,
+                    headers=["Assembly", "Region", "Found", "Total", "% Covered"],
+                    tablefmt="html",
+                    numalign="left",
+                    showindex=False,
+                ),
+                "qv_stats_table": qv_stats_table.to_dict("records"),
+                "qv_stats_table_html": tabulate(
+                    qv_stats_table,
+                    headers=["Assembly", "No Support", "Total", "Error %", "QV"],
+                    tablefmt="html",
+                    numalign="left",
+                    showindex=False,
+                ),
+                "plots": [
+                    {
+                        "hap": hap,
+                        "plot": load_image_as_base64_str(
+                            f"{folder_name}/{data_id}.{hap}.spectra-cn.fl.png"
+                        ),
+                    }
+                    for hap in haplotypes
+                ],
+            }
+        )
+
+    return {"MERQURYFK": sort_list_of_results(data["MERQURYFK"], "individual_id")}
diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html
index 2fbb47ce..31d52c4c 100644
--- a/bin/report_modules/templates/base.html
+++ b/bin/report_modules/templates/base.html
@@ -3,58 +3,130 @@
 
 <body>
   <div class="report-header-wrapper">
-    <div class="report-header">AssemblyQC {{
-      all_stats_dicts['VERSIONS']['Workflow']['plant-food-research-open/assemblyqc'] }}</div>
+    <div class="report-header">
+      AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['plant-food-research-open/assemblyqc'] }}
+    </div>
   </div>
   <div class="tab" id="topNavBarButtons">
+
     <button class="tablinks active" onclick="openTool(event, 'PARAMS')">PARAMS</button>
     <button class="tablinks active" onclick="openTool(event, 'TOOLS')">TOOLS</button>
+
     {% if 'FASTA_VALIDATE' in all_stats_dicts %}
     <button class="tablinks active" onclick="openTool(event, 'FASTA_VALIDATE')">FASTA VALIDATE</button>
     {% endif %}
+
     {% if 'GFF3_VALIDATE' in all_stats_dicts %}
     <button class="tablinks active" onclick="openTool(event, 'GFF3_VALIDATE')">GFF3 VALIDATE</button>
     {% endif %}
+
     {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}
     <button class="tablinks active" onclick="openTool(event, 'NCBI_FCS_ADAPTOR')">NCBI FCS ADAPTOR</button>
-    {% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'NCBI_FCS_GX')">
-      NCBI FCS GX</button>{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}<button class="tablinks active"
-      onclick="openTool(event, 'ASSEMBLATHON_STATS')">
-      ASSEMBLATHON STATS</button>{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}<button
-      class="tablinks active" onclick="openTool(event, 'GENOMETOOLS_GT_STAT')">
-      GENOMETOOLS GT STAT</button>{% endif %} {% if 'BUSCO' in all_stats_dicts %}<button class="tablinks active"
-      onclick="openTool(event, 'BUSCO')">
-      BUSCO</button>{% endif %} {% if 'TIDK' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'TIDK')">
-      TIDK</button>{% endif %} {% if 'LAI' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'LAI')">
-      LAI</button>{% endif %} {% if 'KRAKEN2' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'KRAKEN2')">
-      KRAKEN2</button>{% endif %} {% if 'HIC' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'HIC')">
-      HIC</button>{% endif %} {% if 'SYNTENY_CIRCOS' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'SYNTENY_CIRCOS')">
-      SYNTENY CIRCOS</button>{% endif %}{% if 'SYNTENY_LINEAR' in all_stats_dicts %}<button class="tablinks"
-      onclick="openTool(event, 'SYNTENY_LINEAR')">
-      SYNTENY LINEAR</button>{% endif %}
+    {% endif %}
+
+    {% if 'NCBI_FCS_GX' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'NCBI_FCS_GX')">NCBI FCS GX</button>
+    {% endif %}
+
+    {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}
+    <button class="tablinks active" onclick="openTool(event, 'ASSEMBLATHON_STATS')">ASSEMBLATHON STATS</button>
+    {% endif %}
+
+    {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}
+    <button class="tablinks active" onclick="openTool(event, 'GENOMETOOLS_GT_STAT')">GENOMETOOLS GT STAT</button>
+    {% endif %}
+
+    {% if 'BUSCO' in all_stats_dicts %}
+    <button class="tablinks active" onclick="openTool(event, 'BUSCO')">BUSCO</button>
+    {% endif %}
+
+    {% if 'TIDK' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'TIDK')">TIDK</button>
+    {% endif %}
+
+    {% if 'LAI' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'LAI')">LAI</button>
+    {% endif %}
+
+    {% if 'KRAKEN2' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'KRAKEN2')">KRAKEN2</button>
+    {% endif %}
+
+    {% if 'HIC' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'HIC')">HIC</button>
+    {% endif %}
+
+    {% if 'SYNTENY_CIRCOS' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'SYNTENY_CIRCOS')">SYNTENY CIRCOS</button>
+    {% endif %}
+
+    {% if 'SYNTENY_LINEAR' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'SYNTENY_LINEAR')">SYNTENY LINEAR</button>
+    {% endif %}
+
+    {% if 'MERQURYFK' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'MERQURYFK')">MERQURYFK</button>
+    {% endif %}
   </div>
-  {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{%
-  include 'fasta_validate/fasta_validate.html' %}{% endif %}
-
-  {% if 'GFF3_VALIDATE' in all_stats_dicts %}{% include 'gff3_validate/gff3_validate.html' %}{% endif %}
-
-  {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{%
-  include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{%
-  include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include
-  'assemblathon_stats/assemblathon_stats.html' %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{%
-  include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{%
-  include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{%
-  endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in
-  all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include
-  'hic/hic.html' %}{% endif %} {% if 'SYNTENY_CIRCOS' in all_stats_dicts %}{% include
-  'synteny_circos/synteny_circos.html' %}{% endif %} {% if 'SYNTENY_LINEAR' in all_stats_dicts %}{% include
-  'synteny_linear/synteny_linear.html' %}{% endif %}
+
+  {% include 'params/params.html' %}
+  {% include 'tools/tools.html' %}
+
+  {% if 'FASTA_VALIDATE' in all_stats_dicts %}
+  {%include 'fasta_validate/fasta_validate.html' %}
+  {% endif %}
+
+  {% if 'GFF3_VALIDATE' in all_stats_dicts %}
+  {% include 'gff3_validate/gff3_validate.html' %}
+  {% endif %}
+
+  {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}
+  {% include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}
+  {% endif %}
+
+  {% if 'NCBI_FCS_GX' in all_stats_dicts %}
+  {% include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}
+  {% endif %}
+
+  {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}
+  {% include 'assemblathon_stats/assemblathon_stats.html' %}
+  {% endif %}
+
+  {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}
+  {% include 'genometools_gt_stat/genometools_gt_stat.html' %}
+  {% endif %}
+
+  {% if 'BUSCO' in all_stats_dicts %}
+  {% include 'busco/busco.html' %}
+  {% endif %}
+
+  {% if 'TIDK' in all_stats_dicts %}
+  {% include 'tidk/tidk.html' %}
+  {% endif %}
+
+  {% if 'LAI' in all_stats_dicts %}
+  {% include 'lai/lai.html' %}
+  {% endif %}
+
+  {% if 'KRAKEN2' in all_stats_dicts %}
+  {% include 'kraken2/kraken2.html' %}
+  {% endif %}
+
+  {% if 'HIC' in all_stats_dicts %}
+  {% include 'hic/hic.html' %}
+  {% endif %}
+
+  {% if 'SYNTENY_CIRCOS' in all_stats_dicts %}
+  {% include 'synteny_circos/synteny_circos.html' %}
+  {% endif %}
+
+  {% if 'SYNTENY_LINEAR' in all_stats_dicts %}
+  {% include 'synteny_linear/synteny_linear.html' %}
+  {% endif %}
+
+  {% if 'MERQURYFK' in all_stats_dicts %}
+  {% include 'merquryfk/merquryfk.html' %}
+  {% endif %}
 </body>
 {% include 'js.html' %}
 
diff --git a/bin/report_modules/templates/merquryfk/dropdown.html b/bin/report_modules/templates/merquryfk/dropdown.html
new file mode 100644
index 00000000..d257b4e9
--- /dev/null
+++ b/bin/report_modules/templates/merquryfk/dropdown.html
@@ -0,0 +1,10 @@
+<div class="dropdown">
+  <div class="dropdown_content">
+    <select id="selector_MERQURYFK" onchange="showContent('MERQURYFK')">
+      {% set str_individual_id = 'individual_id' %} {% for item in range(all_stats_dicts["MERQURYFK"]|length) %}
+      <option value="tabcontent_MERQURYFK_{{all_stats_dicts['MERQURYFK'][item]['individual_id']}}">
+        {{ all_stats_dicts['MERQURYFK'][item][str_individual_id] }} {% endfor %}
+      </option>
+    </select>
+  </div>
+</div>
diff --git a/bin/report_modules/templates/merquryfk/merquryfk.html b/bin/report_modules/templates/merquryfk/merquryfk.html
new file mode 100644
index 00000000..8331fbd3
--- /dev/null
+++ b/bin/report_modules/templates/merquryfk/merquryfk.html
@@ -0,0 +1,21 @@
+<div id="MERQURYFK" class="tabcontent" style="display: none">
+  <div class="section-para-wrapper">
+    <p class="section-para">The original Merqury is a collection of R, Java, and shell scripts for producing k-mer
+      analysis plots of genomic sequence data and assemblies with meryl as its core k-mer counter infra-structure.
+      MerquryFK replaces meryl with the FastK k-mer counter suite to considerably speed up analyses.</p>
+    <p class="section-para"><b>References:</b></p>
+    <p class="section-para">
+      <a href="https://github.com/thegenemyers/MERQURY.FK"
+        target="_blank">https://github.com/thegenemyers/MERQURY.FK</a>
+    </p>
+    <p class="section-para">
+      Rhie, A., Walenz, B.P., Koren, S. et al. Merqury: reference-free quality, completeness, and phasing assessment for
+      genome assemblies. Genome Biol 21, 245 (2020). doi: <a href="https://doi.org/10.1186/s13059-020-02134-9"
+        target="_blank">10.1186/s13059-020-02134-9</a>
+    </p>
+    <p class="section-para">
+      <b>Version: {{ all_stats_dicts['VERSIONS']['MERQURYFK_MERQURYFK']['merquryfk'] }}</b>
+    </p>
+  </div>
+  {% include 'merquryfk/dropdown.html' %} {% include 'merquryfk/report_contents.html' %}
+</div>
diff --git a/bin/report_modules/templates/merquryfk/report_contents.html b/bin/report_modules/templates/merquryfk/report_contents.html
new file mode 100644
index 00000000..6578a6f4
--- /dev/null
+++ b/bin/report_modules/templates/merquryfk/report_contents.html
@@ -0,0 +1,36 @@
+{% set vars = {'is_first': True} %}
+{% for item in range(all_stats_dicts["MERQURYFK"]|length) %}
+{% set active_text = 'display: block' if vars.is_first else 'display: none' %}
+<div id="tabcontent_MERQURYFK_{{ all_stats_dicts['MERQURYFK'][item]['individual_id'] }}" class="tabcontent-MERQURYFK"
+  style="{{ active_text }}">
+  <div class="results-section">
+    <div class="section-heading-wrapper">
+      <div class="section-heading">{{ all_stats_dicts['MERQURYFK'][item]['individual_id'] }}</div>
+    </div>
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Completeness stats</b></p>
+    </div>
+    <div class="table-outer">
+      <div class="table-wrapper">{{ all_stats_dicts['MERQURYFK'][item]['completeness_stats_table_html'] }}</div>
+    </div>
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Consensus quality QV stats</b></p>
+    </div>
+    <div class="table-outer">
+      <div class="table-wrapper">{{ all_stats_dicts['MERQURYFK'][item]['qv_stats_table_html'] }}</div>
+    </div>
+
+    {% for subitem in range(all_stats_dicts["MERQURYFK"][item]['plots']|length) %}
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>{{ all_stats_dicts['MERQURYFK'][item]['plots'][subitem]['hap'] }} spectra-cn</b></p>
+    </div>
+    <div class="image-wrapper">
+      <img src="{{ all_stats_dicts['MERQURYFK'][item]['plots'][subitem]['plot'] }}" alt="" />
+    </div>
+    {% endfor %}
+
+  </div>
+</div>
+{% if vars.update({'is_first': False}) %}
+{% endif %}
+{% endfor %}
diff --git a/conf/modules.config b/conf/modules.config
index dd8cc10c..29f2e933 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -187,7 +187,7 @@ process {
         ext.args = '-harvest_out -size 1000000 -time 300'
     }
 
-    withName: CAT_CAT {
+    withName: '.*:FASTA_LTRRETRIEVER_LAI:CAT_CAT' {
         ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" }
     }
 
@@ -298,6 +298,38 @@ process {
         ]
     }
 
+    withName: TAG_ASSEMBLY {
+        ext.prefix = { "${meta.id}.fasta" }
+    }
+
+    withName: '.*:DOWNLOAD_READS:SRATOOLS_FASTERQDUMP' {
+        ext.prefix = { "${meta.id}.sra.fastq" }
+    }
+
+    withName: FASTK_FASTK {
+        ext.args = "-k$params.merqury_kmer_length -t1"
+    }
+
+    withName: MERQURYFK_MERQURYFK {
+        publishDir = [
+            [
+                path: { "${params.outdir}/merquryfk/${meta.id}" },
+                mode: params.publish_dir_mode,
+                pattern: '*.qv'
+            ],
+            [
+                path: { "${params.outdir}/merquryfk/${meta.id}" },
+                mode: params.publish_dir_mode,
+                pattern: '*.completeness.stats'
+            ],
+            [
+                path: { "${params.outdir}/merquryfk/${meta.id}" },
+                mode: params.publish_dir_mode,
+                pattern: '*.*.spectra-cn.fl.png'
+            ]
+        ]
+    }
+
     withName: CREATEREPORT {
         publishDir = [
             path: { "$params.outdir" },
diff --git a/conf/test_full.config b/conf/test_full.config
index c93ba771..9c7b5ad8 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -14,7 +14,7 @@ params {
     config_profile_name         = 'Full test profile'
     config_profile_description  = 'Full test dataset to check pipeline function'
 
-    input                       = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv'
+    input                       = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv'
 
     ncbi_fcs_adaptor_skip       = false
     ncbi_fcs_adaptor_empire     = 'euk'
@@ -39,4 +39,6 @@ params {
 
     synteny_skip                = false
     synteny_xref_assemblies     = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv'
+
+    merqury_skip                = false
 }
diff --git a/docs/contributors.sh b/docs/contributors.sh
index 8dbfc364..f3839348 100755
--- a/docs/contributors.sh
+++ b/docs/contributors.sh
@@ -1,5 +1,5 @@
 #!/usr/bin/env bash
 
-module_authors=$(find ./modules -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '[-" ]' | tr '[:upper:]' '[:lower:]')
-workflow_authors=$(find ./subworkflows -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '[-" ]' | tr '[:upper:]' '[:lower:]')
+module_authors=$(find ./modules -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '"' | tr '[:upper:]' '[:lower:]' | awk '{print $2}')
+workflow_authors=$(find ./subworkflows -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '"' | tr '[:upper:]' '[:lower:]' | awk '{print $2}')
 echo -e "${module_authors}\n${workflow_authors}" | sort -V | uniq | sed -n 's|@\(.*\)|<a href="https://github.com/\1"><img src="https://github.com/\1.png" width="50" height="50"></a>|p'
diff --git a/docs/images/spectra_cn.png b/docs/images/spectra_cn.png
new file mode 100644
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diff --git a/docs/output.md b/docs/output.md
index 8115e697..030ac264 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -23,6 +23,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [Kraken2](#kraken2)
 - [HiC contact map](#hic-contact-map)
 - [Synteny](#synteny)
+- [Merqury.FK](#merquryfk)
 - [Pipeline information](#pipeline-information)
 
 ### FASTA and GFF3 validation
@@ -203,6 +204,22 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 <div align="center"><img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
 <div align="center"><img src="images/synteny_linear.png" alt="AssemblyQC - Linear synteny plot" width="50%"><hr><em>AssemblyQC - Linear synteny plot</em></div>
 
+### Merqury.FK
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `merquryfk/`
+  - `tag1-and-tag2-reads-*`: Results folder for haplotype `tag1` and `tag2`.
+    - `*.completeness.stats`: Assembly completeness statistics
+    - `*.qv`: Assembly consensus quality QV statistics
+    - `*.*.spectra-cn.fl.png`: Spectra-cn plot
+    </details>
+
+[MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) is used for the k-mer analysis.
+
+<div align="center"><img src="images/spectra_cn.png" alt="AssemblyQC - Spectra-cn plot" width="50%"><hr><em>AssemblyQC - Spectra-cn plot</em></div>
+
 ### Pipeline information
 
 <details markdown="1">
diff --git a/docs/parameters.md b/docs/parameters.md
index e9a4df43..b1fd54d3 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -81,6 +81,13 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 | `synteny_color_by_contig`          | Synteny plot is colored by contig                                                                                  | `boolean` | True    |          |        |
 | `synteny_xref_assemblies`          | Reference assemblies for synteny analysis                                                                          | `string`  |         |          |        |
 
+## Merqury options
+
+| Parameter             | Description                      | Type      | Default | Required | Hidden |
+| --------------------- | -------------------------------- | --------- | ------- | -------- | ------ |
+| `merqury_skip`        |                                  | `boolean` | True    |          |        |
+| `merqury_kmer_length` | kmer length for merqury analysis | `integer` | 21      |          |        |
+
 ## Max job request options
 
 Set the top limit for requested resources for any single job.
diff --git a/docs/usage.md b/docs/usage.md
index 0f4214ef..f700ba4f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -9,6 +9,8 @@ You will need to create an assemblysheet with information about the assemblies y
 - `gff3 [Optional]:` GFF3 annotation file if available
 - `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
 - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) when performing synteny analysis
+- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of a genome by MERQURY.FK.
+- `reads_2 [Optional]`: If `reads_1` is a SRA ID, this column is ignored. Otherwise, this column should list the second file of the paired reads.
 
 ## External databases
 
@@ -83,6 +85,10 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
   - `fasta:` FASTA file
   - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column)
 
+### Merqury K-mer analysis
+
+- `merqury_kmer_length`: kmer length for merqury analysis
+
 ## Running the pipeline
 
 The typical command for running the pipeline is as follows:
diff --git a/main.nf b/main.nf
index eb7e9e94..04eef6ad 100755
--- a/main.nf
+++ b/main.nf
@@ -34,6 +34,7 @@ workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
     ch_input
     ch_hic_reads
     ch_xref_assembly
+    ch_reads
     ch_params_as_json
     ch_summary_params_as_json
 
@@ -45,6 +46,7 @@ workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
         ch_input,
         ch_hic_reads,
         ch_xref_assembly,
+        ch_reads,
         ch_params_as_json,
         ch_summary_params_as_json
     )
@@ -80,6 +82,7 @@ workflow {
         PIPELINE_INITIALISATION.out.input,
         PIPELINE_INITIALISATION.out.hic_reads,
         PIPELINE_INITIALISATION.out.xref_assembly,
+        PIPELINE_INITIALISATION.out.reads,
         PIPELINE_INITIALISATION.out.params_as_json,
         PIPELINE_INITIALISATION.out.summary_params_as_json
     )
diff --git a/modules.json b/modules.json
index d387fde5..90ffadab 100644
--- a/modules.json
+++ b/modules.json
@@ -110,6 +110,11 @@
                         "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
                         "installed_by": ["modules"]
                     },
+                    "fastk/fastk": {
+                        "branch": "master",
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "installed_by": ["modules"]
+                    },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
@@ -125,6 +130,11 @@
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
                         "installed_by": ["modules"]
                     },
+                    "merquryfk/merquryfk": {
+                        "branch": "master",
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "installed_by": ["modules"]
+                    },
                     "seqkit/seq": {
                         "branch": "master",
                         "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4",
diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf
index 8deb0edb..087bec70 100644
--- a/modules/local/createreport.nf
+++ b/modules/local/createreport.nf
@@ -17,6 +17,7 @@ process CREATEREPORT {
     path kraken2_outputs, stageAs: 'kraken2_outputs/*'
     path hic_outputs, stageAs: 'hic_outputs/*'
     path synteny_outputs, stageAs: 'synteny_outputs/*'
+    path merqury_outputs, stageAs: 'merquryfk_outputs/*'
     path versions
     val params_json
     val params_summary_json
diff --git a/modules/nf-core/fastk/fastk/environment.yml b/modules/nf-core/fastk/fastk/environment.yml
new file mode 100644
index 00000000..54c932fe
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/environment.yml
@@ -0,0 +1,5 @@
+name: fastk_fastk
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
diff --git a/modules/nf-core/fastk/fastk/main.nf b/modules/nf-core/fastk/fastk/main.nf
new file mode 100644
index 00000000..fec5a4d2
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/main.nf
@@ -0,0 +1,41 @@
+process FASTK_FASTK {
+    tag "$meta.id"
+    label 'process_medium'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.2'
+
+    input:
+    tuple val(meta), path(reads)
+
+    output:
+    tuple val(meta), path("*.hist")                      , emit: hist
+    tuple val(meta), path("*.ktab*", hidden: true)       , emit: ktab, optional: true
+    tuple val(meta), path("*.{prof,pidx}*", hidden: true), emit: prof, optional: true
+    path "versions.yml"                                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "FASTK_FASTK module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    FastK \\
+        $args \\
+        -T$task.cpus \\
+        -M${task.memory.toGiga()} \\
+        -N${prefix}_fk \\
+        $reads
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/fastk/fastk/meta.yml b/modules/nf-core/fastk/fastk/meta.yml
new file mode 100644
index 00000000..7c7f4260
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/meta.yml
@@ -0,0 +1,49 @@
+name: "fastk_fastk"
+description: A fast K-mer counter for high-fidelity shotgun datasets
+keywords:
+  - k-mer
+  - count
+  - histogram
+tools:
+  - "fastk":
+      description: "A fast K-mer counter for high-fidelity shotgun datasets"
+      homepage: "https://github.com/thegenemyers/FASTK"
+      tool_dev_url: "https://github.com/thegenemyers/FASTK"
+      licence: "https://github.com/thegenemyers/FASTK/blob/master/LICENSE"
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - hist:
+      type: file
+      description: Histogram of k-mers
+      pattern: "*.hist"
+  - ktab:
+      type: file
+      description: A sorted table of all canonical k‑mers along with their counts.
+      pattern: "*.ktab"
+  - prof:
+      type: file
+      description: A k‑mer count profile of each sequence in the input data set.
+      pattern: "*.prof"
+authors:
+  - "@mahesh-panchal"
+maintainers:
+  - "@mahesh-panchal"
diff --git a/modules/nf-core/merquryfk/merquryfk/environment.yml b/modules/nf-core/merquryfk/merquryfk/environment.yml
new file mode 100644
index 00000000..44a5ee9b
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/environment.yml
@@ -0,0 +1,5 @@
+name: merquryfk_merquryfk
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
diff --git a/modules/nf-core/merquryfk/merquryfk/main.nf b/modules/nf-core/merquryfk/merquryfk/main.nf
new file mode 100644
index 00000000..ac163dac
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/main.nf
@@ -0,0 +1,58 @@
+process MERQURYFK_MERQURYFK {
+    tag "$meta.id"
+    label 'process_medium'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.2'
+
+    input:
+    tuple val(meta), path(fastk_hist), path(fastk_ktab), path(assembly), path(haplotigs)
+
+    output:
+    tuple val(meta), path("${prefix}.completeness.stats") , emit: stats
+    tuple val(meta), path("${prefix}.*_only.bed")         , emit: bed
+    tuple val(meta), path("${prefix}.*.qv")               , emit: assembly_qv
+    tuple val(meta), path("${prefix}.*.spectra-cn.fl.png"), emit: spectra_cn_fl_png,  optional: true
+    tuple val(meta), path("${prefix}.*.spectra-cn.fl.pdf"), emit: spectra_cn_fl_pdf,  optional: true
+    tuple val(meta), path("${prefix}.*.spectra-cn.ln.png"), emit: spectra_cn_ln_png,  optional: true
+    tuple val(meta), path("${prefix}.*.spectra-cn.ln.pdf"), emit: spectra_cn_ln_pdf,  optional: true
+    tuple val(meta), path("${prefix}.*.spectra-cn.st.png"), emit: spectra_cn_st_png,  optional: true
+    tuple val(meta), path("${prefix}.*.spectra-cn.st.pdf"), emit: spectra_cn_st_pdf,  optional: true
+    tuple val(meta), path("${prefix}.qv")                 , emit: qv
+    tuple val(meta), path("${prefix}.spectra-asm.fl.png") , emit: spectra_asm_fl_png, optional: true
+    tuple val(meta), path("${prefix}.spectra-asm.fl.pdf") , emit: spectra_asm_fl_pdf, optional: true
+    tuple val(meta), path("${prefix}.spectra-asm.ln.png") , emit: spectra_asm_ln_png, optional: true
+    tuple val(meta), path("${prefix}.spectra-asm.ln.pdf") , emit: spectra_asm_ln_pdf, optional: true
+    tuple val(meta), path("${prefix}.spectra-asm.st.png") , emit: spectra_asm_st_png, optional: true
+    tuple val(meta), path("${prefix}.spectra-asm.st.pdf") , emit: spectra_asm_st_pdf, optional: true
+    path "versions.yml"                                   , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    MerquryFK \\
+        $args \\
+        -T$task.cpus \\
+        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }} \\
+        $assembly \\
+        $haplotigs \\
+        $prefix
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+        merquryfk: $MERQURY_VERSION
+        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/merquryfk/merquryfk/meta.yml b/modules/nf-core/merquryfk/merquryfk/meta.yml
new file mode 100644
index 00000000..7d4af796
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/meta.yml
@@ -0,0 +1,112 @@
+name: "merquryfk_merquryfk"
+description: FastK based version of Merqury
+keywords:
+  - Merqury
+  - reference-free
+  - assembly evaluation
+tools:
+  - "merquryfk":
+      description: "FastK based version of Merqury"
+      homepage: "https://github.com/thegenemyers/MERQURY.FK"
+      tool_dev_url: "https://github.com/thegenemyers/MERQURY.FK"
+      licence: "https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fastk_hist:
+      type: file
+      description: A histogram files from the program FastK
+      pattern: "*.hist"
+  - fastk_ktab:
+      type: file
+      description: Histogram ktab files from the program FastK (option -t)
+      pattern: "*.ktab*"
+  - assembly:
+      type: file
+      description: Genome (primary) assembly files (fasta format)
+      pattern: ".fasta"
+  - haplotigs:
+      type: file
+      description: Assembly haplotigs (fasta format)
+      pattern: ".fasta"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - stats:
+      type: file
+      description: Assembly statistics file
+      pattern: "*.completeness.stats"
+  - bed:
+      type: file
+      description: Assembly only kmer positions not supported by reads in bed format
+      pattern: "*_only.bed"
+  - spectra_cn_fl_png:
+      type: file
+      description: "Unstacked copy number spectra filled plot in PNG format"
+      pattern: "*.spectra-cn.fl.png"
+  - spectra_cn_ln_png:
+      type: file
+      description: "Unstacked copy number spectra line plot in PNG format"
+      pattern: "*.spectra-cn.ln.png"
+  - spectra_cn_st_png:
+      type: file
+      description: "Stacked copy number spectra line plot in PNG format"
+      pattern: "*.spectra-cn.st.png"
+  - spectra_asm_fl_png:
+      type: file
+      description: "Unstacked assembly spectra filled plot in PNG format"
+      pattern: "*.spectra-asm.fl.png"
+  - spectra_asm_ln_png:
+      type: file
+      description: "Unstacked assembly spectra line plot in PNG format"
+      pattern: "*.spectra-asm.ln.png"
+  - spectra_asm_st_png:
+      type: file
+      description: "Stacked assembly spectra line plot in PNG format"
+      pattern: "*.spectra-asm.st.png"
+  - spectra_cn_fl_pdf:
+      type: file
+      description: "Unstacked copy number spectra filled plot in PDF format"
+      pattern: "*.spectra-cn.fl.pdf"
+  - spectra_cn_ln_pdf:
+      type: file
+      description: "Unstacked copy number spectra line plot in PDF format"
+      pattern: "*.spectra-cn.ln.pdf"
+  - spectra_cn_st_pdf:
+      type: file
+      description: "Stacked copy number spectra line plot in PDF format"
+      pattern: "*.spectra-cn.st.pdf"
+  - spectra_asm_fl_pdf:
+      type: file
+      description: "Unstacked assembly spectra filled plot in PDF format"
+      pattern: "*.spectra-asm.fl.pdf"
+  - spectra_asm_ln_pdf:
+      type: file
+      description: "Unstacked assembly spectra line plot in PDF format"
+      pattern: "*.spectra-asm.ln.pdf"
+  - spectra_asm_st_pdf:
+      type: file
+      description: "Stacked assembly spectra line plot in PDF format"
+      pattern: "*.spectra-asm.st.pdf"
+  - assembly_qv:
+      type: file
+      description: "error and qv table for each scaffold of the assembly"
+      pattern: "*.qv"
+  - qv:
+      type: file
+      description: "error and qv of each assembly as a whole"
+      pattern: "*.qv"
+authors:
+  - "@mahesh-panchal"
+maintainers:
+  - "@mahesh-panchal"
diff --git a/modules/pfr/bwa/index/environment.yml b/modules/pfr/bwa/index/environment.yml
index 5d3cb323..126e0034 100644
--- a/modules/pfr/bwa/index/environment.yml
+++ b/modules/pfr/bwa/index/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::bwa=0.7.17
+  - bioconda::bwa=0.7.18
diff --git a/modules/pfr/bwa/index/main.nf b/modules/pfr/bwa/index/main.nf
index 24b5a2ea..2e48b6ca 100644
--- a/modules/pfr/bwa/index/main.nf
+++ b/modules/pfr/bwa/index/main.nf
@@ -4,8 +4,8 @@ process BWA_INDEX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bwa:0.7.17--hed695b0_7' :
-        'biocontainers/bwa:0.7.17--hed695b0_7' }"
+        'https://depot.galaxyproject.org/singularity/bwa:0.7.18--he4a0461_0' :
+        'biocontainers/bwa:0.7.18--he4a0461_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/nextflow.config b/nextflow.config
index 5a8128aa..82b2b89c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -61,6 +61,10 @@ params {
     synteny_color_by_contig             = true
     synteny_xref_assemblies             = null
 
+    // Merqury options
+    merqury_skip                        = true
+    merqury_kmer_length                 = 21
+
     // Output options
     outdir                              = './results'
     email                               = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 432f3f82..36f7556e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -266,6 +266,25 @@
                 }
             }
         },
+        "merqury_options": {
+            "title": "Merqury options",
+            "type": "object",
+            "description": "",
+            "default": "",
+            "properties": {
+                "merqury_skip": {
+                    "type": "boolean",
+                    "default": true,
+                    "fa_icon": "fas fa-forward"
+                },
+                "merqury_kmer_length": {
+                    "type": "integer",
+                    "default": 21,
+                    "description": "kmer length for merqury analysis",
+                    "minimum": 3
+                }
+            }
+        },
         "max_job_request_options": {
             "title": "Max job request options",
             "type": "object",
@@ -459,6 +478,9 @@
         {
             "$ref": "#/definitions/synteny_options"
         },
+        {
+            "$ref": "#/definitions/merqury_options"
+        },
         {
             "$ref": "#/definitions/max_job_request_options"
         },
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 52d742bc..489b1c36 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -121,6 +121,23 @@ workflow PIPELINE_INITIALISATION {
                                                 [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ]
                                             }
 
+    ch_reads                                = params.merqury_skip
+                                            ? Channel.empty()
+                                            : ch_input_validated
+                                            | map { input_data ->
+                                                def tag     = input_data[0]
+                                                def reads_1 = input_data[5]
+                                                def reads_2 = input_data[6]
+
+                                                reads_1
+                                                ? extractReadsTuple ( tag, reads_1, reads_2 )
+                                                : null
+                                            }
+                                            | groupTuple
+                                            | map { fid, metas, reads ->
+                                                validateAndNormaliseReadsTupe ( fid, metas, reads )
+                                            }
+
     // Initialise parameter channels
     ch_params_as_json                       = Channel.of ( jsonifyParams ( params ) )
     ch_summary_params_as_json               = Channel.of ( jsonifySummaryParams ( summary_params ) )
@@ -129,6 +146,7 @@ workflow PIPELINE_INITIALISATION {
     input                                   = ch_input_validated
     hic_reads                               = ch_hic_reads
     xref_assembly                           = ch_xref_assembly_validated
+    reads                                   = ch_reads
     params_as_json                          = ch_params_as_json
     summary_params_as_json                  = ch_summary_params_as_json
     versions                                = ch_versions
@@ -264,3 +282,86 @@ def jsonifySummaryParams(params) {
 
     return JsonOutput.toJson(summary).toString()
 }
+
+def extractReadsTuple(tag, reads_1, reads_2) {
+    if ( reads_1 && reads_2 ) {
+        return [
+            [ fid: file(reads_1).name ],
+            [
+                id: tag,
+                single_end: false,
+                is_sra: false
+            ],
+            [
+                reads_1,
+                reads_2,
+            ]
+        ]
+    }
+
+    if ( "$reads_1".find(/^SRR[0-9]*$/) ) {
+        return [
+            [ fid: "$reads_1" ],
+            [
+                id: tag,
+                single_end: false,
+                is_sra: true
+            ],
+            reads_1
+        ]
+    }
+
+    return [
+        [ fid: file(reads_1).name ],
+        [
+            id: tag,
+            single_end: true,
+            is_sra: false
+        ],
+        [
+            reads_1
+        ]
+    ]
+}
+
+def validateAndNormaliseReadsTupe ( fid, metas, reads ) {
+
+    def tags        = metas.collect { it.id }.flatten()
+    def endedness   = metas.collect { it.single_end }.flatten()
+
+    // Validate
+    if ( endedness.unique().size() != 1 ) {
+        error("Please check input assemblysheet -> Following assemblies have different reads_1 and reads_2: ${tags}")
+    }
+
+    if ( tags.size() > 2 ) {
+        error("Please check input assemblysheet -> More than two assemblies (${tags}) are in the same read group: ${fid}")
+    }
+
+    def individualID = tags.join('-and-') + "-reads-" + fid.fid.replaceAll(/\./, '_')
+
+    if ( metas.first().is_sra ) { // SRA
+        return [
+            [ id:individualID, single_end:false, is_sra:true, assemblies:tags ],
+            reads.first()
+        ]
+    }
+
+    if ( endedness.unique().first() ) { // Single ended
+        return [
+            [ id:individualID, single_end:true, is_sra:false, assemblies:tags ],
+            reads.first().collect { file(it, checkIfExists: true) }
+        ]
+    }
+
+    def reads_2 = reads.collect { it[1] }
+
+    if ( reads_2.unique().size() != 1 ) {
+        error("Please check input assemblysheet -> Following assemblies have different reads_1 and reads_2: ${tags}")
+    }
+
+    return [
+        [ id:individualID, single_end:false, is_sra:false, assemblies:tags ],
+        reads.first().collect { file(it, checkIfExists: true) }
+    ]
+}
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 892c142b..f90e4354 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -19,11 +19,16 @@ include { ASSEMBLATHON_STATS                } from '../modules/local/assemblatho
 include { FASTA_BUSCO_PLOT                  } from '../subworkflows/local/fasta_busco_plot'
 include { FASTA_LTRRETRIEVER_LAI            } from '../subworkflows/pfr/fasta_ltrretriever_lai/main'
 include { FASTA_KRAKEN2                     } from '../subworkflows/local/fasta_kraken2'
-include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_HIC } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
 include { FQ2HIC                            } from '../subworkflows/local/fq2hic'
+include { CAT_CAT as TAG_ASSEMBLY           } from '../modules/pfr/cat/cat/main'
 include { FASTA_SYNTENY                     } from '../subworkflows/local/fasta_synteny'
+include { FASTK_FASTK                       } from '../modules/nf-core/fastk/fastk/main'
+include { MERQURYFK_MERQURYFK               } from '../modules/nf-core/merquryfk/merquryfk/main'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
+include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_HIC      } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
+include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_READS    } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -36,6 +41,7 @@ workflow ASSEMBLYQC {
     ch_input
     ch_hic_reads
     ch_xref_assembly
+    ch_reads
     ch_params_as_json
     ch_summary_params_as_json
 
@@ -407,6 +413,84 @@ workflow ASSEMBLYQC {
     ch_synteny_plots                        = FASTA_SYNTENY.out.png.mix(FASTA_SYNTENY.out.html)
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
+    // MODULE: CAT_CAT as TAG_ASSEMBLY
+    TAG_ASSEMBLY (
+        ch_clean_assembly.map { tag, fa -> [ [ id: tag ], fa ] }
+    )
+
+    ch_clean_assembly_tagged                = TAG_ASSEMBLY.out.file_out
+    ch_versions                             = ch_versions.mix(TAG_ASSEMBLY.out.versions)
+
+    // MODULE: FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_READS
+    ch_reads_assemblies                     = ch_reads
+                                            | combine(
+                                                ch_clean_assembly_tagged
+                                                | map { [ it ] }
+                                                | collect
+                                                | map { [ it ] }
+                                            )
+                                            | map { meta, fq, assemblies ->
+                                                [
+                                                    [ id: meta.id, single_end: meta.single_end, is_sra: meta.is_sra ],
+                                                    fq,
+                                                    assemblies
+                                                        .findAll { meta2, fasta -> meta2.id in meta.assemblies }
+                                                        .collect { meta2, fasta -> fasta }
+                                                        .flatten()
+                                                        .sort(false)
+                                                ]
+                                            }
+
+    ch_reads_branch                         = ch_reads_assemblies
+                                            | map { meta, fq, fastas -> [ meta, fq ] }
+                                            | branch { meta, fq ->
+                                                sra: meta.is_sra
+                                                rest: ! meta.is_sra
+                                            }
+
+    DOWNLOAD_READS(
+        ch_reads_branch.sra,
+        []
+    )
+
+    ch_reads_files                          = DOWNLOAD_READS.out.reads
+                                            | mix(ch_reads_branch.rest)
+
+    ch_versions                             = ch_versions.mix(DOWNLOAD_READS.out.versions)
+
+    // MODULE: FASTK_FASTK
+    FASTK_FASTK ( ch_reads_files )
+
+    ch_reads_fastk_hist                     = FASTK_FASTK.out.hist
+    ch_reads_fastk                          = FASTK_FASTK.out.ktab
+    ch_versions                             = ch_versions.mix(FASTK_FASTK.out.versions.first())
+
+    // MODULE: MERQURYFK_MERQURYFK
+    ch_merqury_assemblies                   = ch_reads_assemblies
+                                            | map { meta, fq, fastas -> [ meta, fastas ] }
+
+    ch_merqury_inputs                       = ch_reads_fastk_hist
+                                            | join(
+                                                ch_reads_fastk
+                                            )
+                                            | join(
+                                                ch_merqury_assemblies
+                                            )
+                                            | map { meta, hist, ktab, fastas ->
+                                                [ meta, hist, ktab, fastas, [] ]
+                                            }
+
+    MERQURYFK_MERQURYFK ( ch_merqury_inputs )
+
+    ch_merqury_qv                           = MERQURYFK_MERQURYFK.out.qv
+    ch_merqury_stats                        = MERQURYFK_MERQURYFK.out.stats
+    ch_merqury_spectra_cn_fl_png            = MERQURYFK_MERQURYFK.out.spectra_cn_fl_png
+    ch_merqury_outputs                      = ch_merqury_qv
+                                            | mix(ch_merqury_stats)
+                                            | mix(ch_merqury_spectra_cn_fl_png)
+                                            | flatMap { meta, data -> data }
+    ch_versions                             = ch_versions.mix(MERQURYFK_MERQURYFK.out.versions.first())
+
     // Collate and save software versions
     ch_versions                             = ch_versions
                                             | unique
@@ -436,6 +520,7 @@ workflow ASSEMBLYQC {
         ch_kraken2_plot                     .collect().ifEmpty([]),
         ch_hic_html                         .collect().ifEmpty([]),
         ch_synteny_plots                    .collect().ifEmpty([]),
+        ch_merqury_outputs                  .collect().ifEmpty([]),
         ch_versions_yml,
         ch_params_as_json,
         ch_summary_params_as_json

From 9baec0f9b18aacc268a0fb20479e55b09522cd69 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 16 May 2024 13:51:25 +1200
Subject: [PATCH 36/76] Template update for nf-core/tools version 2.14.1

---
 .editorconfig                                 |   6 +-
 .github/PULL_REQUEST_TEMPLATE.md              |   2 +-
 .github/workflows/ci.yml                      |   4 +-
 .github/workflows/download_pipeline.yml       |  22 ++-
 .github/workflows/fix-linting.yml             |   6 +-
 .github/workflows/linting.yml                 |  19 +-
 .github/workflows/linting_comment.yml         |   2 +-
 .github/workflows/release-announcements.yml   |   6 +-
 .nf-core.yml                                  |   1 +
 .pre-commit-config.yaml                       |   3 +
 CHANGELOG.md                                  |   2 +-
 README.md                                     |   2 +-
 assets/multiqc_config.yml                     |   4 +-
 conf/base.config                              |   3 -
 conf/modules.config                           |   8 -
 conf/test.config                              |   2 +-
 conf/test_full.config                         |   2 +-
 docs/usage.md                                 |   2 +
 modules.json                                  |   4 +-
 modules/nf-core/fastqc/main.nf                |   6 +
 nextflow.config                               | 178 +++++++++---------
 nextflow_schema.json                          |   7 +
 pyproject.toml                                |  15 --
 .../utils_nfcore_assemblyqc_pipeline/main.nf  |  16 +-
 .../nf-core/utils_nfcore_pipeline/main.nf     |   8 +-
 workflows/assemblyqc.nf                       |  46 +++--
 26 files changed, 212 insertions(+), 164 deletions(-)
 delete mode 100644 pyproject.toml

diff --git a/.editorconfig b/.editorconfig
index dd9ffa53..72dda289 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -28,10 +28,6 @@ indent_style = unset
 [/assets/email*]
 indent_size = unset
 
-# ignore Readme
-[README.md]
-indent_style = unset
-
-# ignore python
+# ignore python and markdown
 [*.{py,md}]
 indent_style = unset
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 12f22500..3da295ca 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/plant-food-r
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md)
 - [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
 - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index bd73a829..81e48363 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,10 +28,10 @@ jobs:
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
         with:
           version: "${{ matrix.NXF_VER }}"
 
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 08622fd5..2d20d644 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -14,6 +14,8 @@ on:
   pull_request:
     types:
       - opened
+      - edited
+      - synchronize
     branches:
       - master
   pull_request_target:
@@ -28,11 +30,14 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
       - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
         with:
@@ -65,8 +70,17 @@ jobs:
       - name: Inspect download
         run: tree ./${{ env.REPOTITLE_LOWERCASE }}
 
-      - name: Run the downloaded pipeline
+      - name: Run the downloaded pipeline (stub)
+        id: stub_run_pipeline
+        continue-on-error: true
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
+      - name: Run the downloaded pipeline (stub run not supported)
+        id: run_pipeline
+        if: ${{ job.steps.stub_run_pipeline.status == failure() }}
+        env:
+          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_HOME_MOUNT: true
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 80625a8f..31ca9786 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -32,9 +32,9 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       # Install and run pre-commit
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 073e1876..1fcafe88 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,13 +14,12 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
-      - name: Set up Python 3.11
-        uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - name: Set up Python 3.12
+        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
-          cache: "pip"
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
@@ -32,14 +31,14 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
 
       - name: Install dependencies
@@ -60,7 +59,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
+        uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index b706875f..40acc23f 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3
+        uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index d468aeaa..03ecfcf7 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -12,7 +12,7 @@ jobs:
       - name: get topics and convert to hashtags
         id: get_topics
         run: |
-          curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT
+          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')"  >> $GITHUB_OUTPUT
 
       - uses: rzr/fediverse-action@master
         with:
@@ -25,13 +25,13 @@ jobs:
 
             Please see the changelog: ${{ github.event.release.html_url }}
 
-            ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics
+            ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
 
   send-tweet:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
           python-version: "3.10"
       - name: Install dependencies
diff --git a/.nf-core.yml b/.nf-core.yml
index 61d19b4d..12896981 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -18,6 +18,7 @@ lint:
   nextflow_config:
     - manifest.name
     - manifest.homePage
+nf_core_version: 2.14.1
 repository_type: pipeline
 template:
   prefix: plant-food-research-open
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index af57081f..4dc0f1dc 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -3,6 +3,9 @@ repos:
     rev: "v3.1.0"
     hooks:
       - id: prettier
+        additional_dependencies:
+          - prettier@3.2.5
+
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
     rev: "2.7.3"
     hooks:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 59ee0da8..c0ebecc9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0 - [date]
+## v2.0.0+dev - [date]
 
 Initial release of plant-food-research-open/assemblyqc, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/README.md b/README.md
index 19194afb..5ea7ea72 100644
--- a/README.md
+++ b/README.md
@@ -6,7 +6,7 @@
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
-[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
+[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc)
 
 ## Introduction
 
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 759adecd..0015bfa8 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,8 +1,6 @@
 report_comment: >
-  
-  This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/releases/tag/2.0.0" target="_blank">plant-food-research-open/assemblyqc</a>
+  This report has been generated by the <a href="https://github.com/plant-food-research-open/assemblyqc/tree/dev" target="_blank">plant-food-research-open/assemblyqc</a>
   analysis pipeline.
-  
 report_section_order:
   "plant-food-research-open-assemblyqc-methods-description":
     order: -1000
diff --git a/conf/base.config b/conf/base.config
index fad3aa91..faaec489 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -59,7 +59,4 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
-    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
-        cache = false
-    }
 }
diff --git a/conf/modules.config b/conf/modules.config
index e3ea8fa6..d203d2b6 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -22,14 +22,6 @@ process {
         ext.args = '--quiet'
     }
 
-    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            pattern: '*_versions.yml'
-        ]
-    }
-
     withName: 'MULTIQC' {
         ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
diff --git a/conf/test.config b/conf/test.config
index 2bc1ad8e..6bfdc87a 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -22,7 +22,7 @@ params {
     // Input data
     // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
+    input  = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
 
     // Genome references
     genome = 'R64-1-1'
diff --git a/conf/test_full.config b/conf/test_full.config
index 63d2687c..931bacf5 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -17,7 +17,7 @@ params {
     // Input data for full size test
     // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
+    input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
 
     // Genome references
     genome = 'R64-1-1'
diff --git a/docs/usage.md b/docs/usage.md
index 1a98b70a..9b9c7ebd 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -154,6 +154,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
 - `apptainer`
   - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
+- `wave`
+  - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later).
 - `conda`
   - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
 
diff --git a/modules.json b/modules.json
index 1d7adb5b..8558f6d2 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
+                        "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
@@ -26,7 +26,7 @@
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfvalidation_plugin": {
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 9e19a74c..d79f1c86 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -25,6 +25,11 @@ process FASTQC {
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
     def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB')
+    // FastQC memory value allowed range (100 - 10000)
+    def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
+
     """
     printf "%s %s\\n" $rename_to | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
@@ -33,6 +38,7 @@ process FASTQC {
     fastqc \\
         $args \\
         --threads $task.cpus \\
+        --memory $fastqc_memory \\
         $renamed_files
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/nextflow.config b/nextflow.config
index cbc0aa8d..6bae5f67 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,7 +16,8 @@ params {
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
     igenomes_ignore            = false
-    fasta                      = null// MultiQC options
+
+    // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     multiqc_logo               = null
@@ -24,15 +25,16 @@ params {
     multiqc_methods_description = null
 
     // Boilerplate options
-    outdir                     = null
-    publish_dir_mode           = 'copy'
-    email                      = null
-    email_on_fail              = null
-    plaintext_email            = false
-    monochrome_logs            = false
-    hook_url                   = null
-    help                       = false
-    version                    = false
+    outdir                       = null
+    publish_dir_mode             = 'copy'
+    email                        = null
+    email_on_fail                = null
+    plaintext_email              = false
+    monochrome_logs              = false
+    hook_url                     = null
+    help                         = false
+    version                      = false
+    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
 
     // Config options
     config_profile_name        = null
@@ -68,103 +70,109 @@ try {
 }
 
 // Load plant-food-research-open/assemblyqc custom profiles from different institutions.
-// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs!
-// try {
-//   includeConfig "${params.custom_config_base}/pipeline/assemblyqc.config"
-// } catch (Exception e) {
-//   System.err.println("WARNING: Could not load nf-core/config/assemblyqc profiles: ${params.custom_config_base}/pipeline/assemblyqc.config")
-// }
+try {
+    includeConfig "${params.custom_config_base}/pipeline/assemblyqc.config"
+} catch (Exception e) {
+    System.err.println("WARNING: Could not load nf-core/config/assemblyqc profiles: ${params.custom_config_base}/pipeline/assemblyqc.config")
+}
 profiles {
     debug {
-        dumpHashes             = true
-        process.beforeScript   = 'echo $HOSTNAME'
-        cleanup                = false
+        dumpHashes              = true
+        process.beforeScript    = 'echo $HOSTNAME'
+        cleanup                 = false
         nextflow.enable.configProcessNamesValidation = true
     }
     conda {
-        conda.enabled          = true
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        channels               = ['conda-forge', 'bioconda', 'defaults']
-        apptainer.enabled      = false
+        conda.enabled           = true
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        conda.channels          = ['conda-forge', 'bioconda', 'defaults']
+        apptainer.enabled       = false
     }
     mamba {
-        conda.enabled          = true
-        conda.useMamba         = true
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        conda.enabled           = true
+        conda.useMamba          = true
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     docker {
-        docker.enabled         = true
-        conda.enabled          = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
-        docker.runOptions      = '-u $(id -u):$(id -g)'
+        docker.enabled          = true
+        conda.enabled           = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
+        docker.runOptions       = '-u $(id -u):$(id -g)'
     }
     arm {
-        docker.runOptions      = '-u $(id -u):$(id -g) --platform=linux/amd64'
+        docker.runOptions       = '-u $(id -u):$(id -g) --platform=linux/amd64'
     }
     singularity {
-        singularity.enabled    = true
-        singularity.autoMounts = true
-        conda.enabled          = false
-        docker.enabled         = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        singularity.enabled     = true
+        singularity.autoMounts  = true
+        conda.enabled           = false
+        docker.enabled          = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     podman {
-        podman.enabled         = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        podman.enabled          = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     shifter {
-        shifter.enabled        = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        shifter.enabled         = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     charliecloud {
-        charliecloud.enabled   = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        apptainer.enabled      = false
+        charliecloud.enabled    = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        apptainer.enabled       = false
     }
     apptainer {
-        apptainer.enabled      = true
-        apptainer.autoMounts   = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
+        apptainer.enabled       = true
+        apptainer.autoMounts    = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+    }
+    wave {
+        apptainer.ociAutoPull   = true
+        singularity.ociAutoPull = true
+        wave.enabled            = true
+        wave.freeze             = true
+        wave.strategy           = 'conda,container'
     }
     gitpod {
-        executor.name          = 'local'
-        executor.cpus          = 4
-        executor.memory        = 8.GB
+        executor.name           = 'local'
+        executor.cpus           = 4
+        executor.memory         = 8.GB
     }
     test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
@@ -231,7 +239,7 @@ manifest {
     description     = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '2.0.0'
+    version         = '2.0.0+dev'
     doi             = ''
 }
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index dda3a0f5..971d65a2 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -265,6 +265,13 @@
                     "description": "Validation of parameters in lenient more.",
                     "hidden": true,
                     "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
+                },
+                "pipelines_testdata_base_path": {
+                    "type": "string",
+                    "fa_icon": "far fa-check-circle",
+                    "description": "Base URL or local path to location of pipeline test dataset files",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/",
+                    "hidden": true
                 }
             }
         }
diff --git a/pyproject.toml b/pyproject.toml
deleted file mode 100644
index 56110621..00000000
--- a/pyproject.toml
+++ /dev/null
@@ -1,15 +0,0 @@
-# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff.
-# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
-[tool.ruff]
-line-length = 120
-target-version = "py38"
-cache-dir = "~/.cache/ruff"
-
-[tool.ruff.lint]
-select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
-
-[tool.ruff.lint.isort]
-known-first-party = ["nf_core"]
-
-[tool.ruff.lint.per-file-ignores]
-"__init__.py" = ["E402", "F401"]
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 0e9efa87..e8fa98a5 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -140,6 +140,10 @@ workflow PIPELINE_COMPLETION {
             imNotification(summary_params, hook_url)
         }
     }
+
+    workflow.onError {
+        log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting"
+    }
 }
 
 /*
@@ -230,8 +234,16 @@ def methodsDescriptionText(mqc_methods_yaml) {
     meta["manifest_map"] = workflow.manifest.toMap()
 
     // Pipeline DOI
-    meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
-    meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
+    if (meta.manifest_map.doi) {
+        // Using a loop to handle multiple DOIs
+        // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
+        // Removing ` ` since the manifest.doi is a string and not a proper list
+        def temp_doi_ref = ""
+        String[] manifest_doi = meta.manifest_map.doi.tokenize(",")
+        for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: <a href=\'https://doi.org/${doi_ref.replace("https://doi.org/", "").replace(" ", "")}\'>${doi_ref.replace("https://doi.org/", "").replace(" ", "")}</a>), "
+        meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2)
+    } else meta["doi_text"] = ""
+    meta["nodoi_text"] = meta.manifest_map.doi ? "" : "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
 
     // Tool references
     meta["tool_citations"] = ""
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index a8b55d6f..14558c39 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) {
 // Citation string for pipeline
 //
 def workflowCitation() {
+    def temp_doi_ref = ""
+    String[] manifest_doi = workflow.manifest.doi.tokenize(",")
+    // Using a loop to handle multiple DOIs
+    // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
+    // Removing ` ` since the manifest.doi is a string and not a proper list
+    for (String doi_ref: manifest_doi) temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
     return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
         "* The pipeline\n" +
-        "  ${workflow.manifest.doi}\n\n" +
+        temp_doi_ref + "\n" +
         "* The nf-core framework\n" +
         "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
         "* Software dependencies\n" +
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index cd30a061..b47addae 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -40,22 +40,44 @@ workflow ASSEMBLYQC {
     // Collate and save software versions
     //
     softwareVersionsToYAML(ch_versions)
-        .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true)
-        .set { ch_collated_versions }
+        .collectFile(
+            storeDir: "${params.outdir}/pipeline_info",
+            name: 'nf_core_pipeline_software_mqc_versions.yml',
+            sort: true,
+            newLine: true
+        ).set { ch_collated_versions }
 
     //
     // MODULE: MultiQC
     //
-    ch_multiqc_config                     = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    ch_multiqc_custom_config              = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
-    ch_multiqc_logo                       = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty()
-    summary_params                        = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary                   = Channel.value(paramsSummaryMultiqc(summary_params))
-    ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description))
-    ch_multiqc_files                      = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files                      = ch_multiqc_files.mix(ch_collated_versions)
-    ch_multiqc_files                      = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false))
+    ch_multiqc_config        = Channel.fromPath(
+        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config = params.multiqc_config ?
+        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        Channel.empty()
+    ch_multiqc_logo          = params.multiqc_logo ?
+        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        Channel.empty()
+
+    summary_params      = paramsSummaryMap(
+        workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+        file(params.multiqc_methods_description, checkIfExists: true) :
+        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(
+        methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_methods_description.collectFile(
+            name: 'methods_description_mqc.yaml',
+            sort: true
+        )
+    )
 
     MULTIQC (
         ch_multiqc_files.collect(),

From 62e58966cefd7105d3d1c0512f7167640c0ca002 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 16 May 2024 14:25:16 +1200
Subject: [PATCH 37/76] Fixed linting issues

---
 .github/workflows/linting.yml |  3 +++
 nextflow.config               |  2 +-
 nextflow_schema.json          | 34 +++++-----------------------------
 version_check.sh              |  2 +-
 4 files changed, 10 insertions(+), 31 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 3c80a33f..05605419 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -57,10 +57,13 @@ jobs:
             --markdown lint_results.md \
             --key actions_ci \
             --key actions_schema_validation \
+            --key base_config \
             --key files_exist \
             --key files_unchanged \
             --key merge_markers \
+            --key modules_config \
             --key nextflow_config \
+            --key nfcore_yml \
             --key pipeline_name_conventions \
             --key pipeline_todos \
             --key readme \
diff --git a/nextflow.config b/nextflow.config
index f28a80d3..9203b2a6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -277,7 +277,7 @@ manifest {
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
     version         = '2.0.0+dev'
-    doi             = '10.5281/zenodo.10647870'
+    doi             = 'https://doi.org/10.5281/zenodo.10647870'
 }
 
 // Load modules.config for DSL2 module specific options
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f58a28f1..28754b9f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -35,10 +35,7 @@
                     "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$"
                 }
             },
-            "required": [
-                "input",
-                "outdir"
-            ]
+            "required": ["input", "outdir"]
         },
         "general_stats_options": {
             "title": "General stats options",
@@ -67,10 +64,7 @@
                 },
                 "ncbi_fcs_adaptor_empire": {
                     "type": "string",
-                    "enum": [
-                        "euk",
-                        "prok"
-                    ],
+                    "enum": ["euk", "prok"],
                     "description": "Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes"
                 },
                 "ncbi_fcs_gx_skip": {
@@ -111,14 +105,7 @@
                 },
                 "busco_mode": {
                     "type": "string",
-                    "enum": [
-                        "geno",
-                        "tran",
-                        "prot",
-                        "genome",
-                        "transcriptome",
-                        "proteins"
-                    ],
+                    "enum": ["geno", "tran", "prot", "genome", "transcriptome", "proteins"],
                     "description": "BUSCO mode: 'geno' for genome, 'tran' for transcriptome, 'prot' for proteins"
                 },
                 "busco_lineage_datasets": {
@@ -240,11 +227,7 @@
                     "type": "string",
                     "default": "both",
                     "description": "Synteny plot type: 'linear', 'circos', or 'both'",
-                    "enum": [
-                        "both",
-                        "linear",
-                        "circos"
-                    ]
+                    "enum": ["both", "linear", "circos"]
                 },
                 "synteny_between_input_assemblies": {
                     "type": "boolean",
@@ -406,14 +389,7 @@
                     "default": "copy",
                     "description": "Method used to save pipeline results to output directory.",
                     "fa_icon": "fas fa-copy",
-                    "enum": [
-                        "symlink",
-                        "rellink",
-                        "link",
-                        "copy",
-                        "copyNoFollow",
-                        "move"
-                    ],
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
                     "hidden": true
                 },
                 "email_on_fail": {
diff --git a/version_check.sh b/version_check.sh
index e23860d3..23cf619c 100755
--- a/version_check.sh
+++ b/version_check.sh
@@ -10,5 +10,5 @@ fi
 
 # Check CHANGELOG version
 
-grep "## $config_version - " CHANGELOG.md >/dev/null \
+head -10 CHANGELOG.md | grep "## v$config_version - " >/dev/null \
     || (echo 'Failed to match CHANGELOG version'; exit 1)

From 3f5bb21d427c3c760a07e3411cbbaf873fb28603 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 16 May 2024 14:31:34 +1200
Subject: [PATCH 38/76] Updated fasta_explore_search_plot_tidk

---
 CHANGELOG.md                                  |  2 +-
 modules.json                                  |  6 +--
 modules/nf-core/seqkit/seq/environment.yml    |  2 +-
 modules/nf-core/seqkit/seq/main.nf            |  4 +-
 modules/nf-core/seqkit/seq/tests/main.nf.test |  4 --
 .../seqkit/seq/tests/main.nf.test.snap        | 48 ++++++++++--------
 modules/nf-core/seqkit/sort/environment.yml   |  2 +-
 modules/nf-core/seqkit/sort/main.nf           |  4 +-
 .../nf-core/seqkit/sort/tests/main.nf.test    |  4 --
 .../seqkit/sort/tests/main.nf.test.snap       | 48 ++++++++++--------
 .../tests/main.nf.test.snap                   | 50 ++++++++++++-------
 11 files changed, 97 insertions(+), 77 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5cfc2050..90c44b94 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,7 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 4. Added a launcher script for local testing `local_assemblyqc`
 5. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
 6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
-7. Updated modules and sub-workflows: `bwa/index`, `bwa/mem`, `cat/cat`, `custom/checkgff3fastacorrespondence`, `custom/restoregffids`, `custom/shortenfastaids`, `gt/gff3`, `gt/gff3validator`, `gt/stat`, `ltrfinder`, `ltrharvest`, `ltrretriever/lai`, `ltrretriever/ltrretriever`, `samblaster`, `fasta_ltrretriever_lai`, `fastq_bwa_mem_samblaster`, `gff3_validate`, `custom/sratoolsncbisettings`, `fastp`, `fastqc`, `untar`
+7. Updated modules and sub-workflows: `bwa/index`, `bwa/mem`, `cat/cat`, `custom/checkgff3fastacorrespondence`, `custom/restoregffids`, `custom/shortenfastaids`, `gt/gff3`, `gt/gff3validator`, `gt/stat`, `ltrfinder`, `ltrharvest`, `ltrretriever/lai`, `ltrretriever/ltrretriever`, `samblaster`, `fasta_ltrretriever_lai`, `fastq_bwa_mem_samblaster`, `gff3_validate`, `custom/sratoolsncbisettings`, `fastp`, `fastqc`, `untar`, `seqkit/seq`, `seqkit/sort`, `fasta_explore_search_plot_tidk`
 8. Locally bumped `bwa/index` version to `biocontainers/bwa:0.7.18--he4a0461_0`
 9. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
 10. Updated `fcs-adaptor` to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
diff --git a/modules.json b/modules.json
index 90ffadab..59b548a7 100644
--- a/modules.json
+++ b/modules.json
@@ -137,12 +137,12 @@
                     },
                     "seqkit/seq": {
                         "branch": "master",
-                        "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4",
+                        "git_sha": "2be41ca2cc780eca4293d1b0dd3850b0b7ac40a3",
                         "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "seqkit/sort": {
                         "branch": "master",
-                        "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d",
+                        "git_sha": "2be41ca2cc780eca4293d1b0dd3850b0b7ac40a3",
                         "installed_by": ["fasta_explore_search_plot_tidk"]
                     },
                     "sratools/fasterqdump": {
@@ -181,7 +181,7 @@
                 "nf-core": {
                     "fasta_explore_search_plot_tidk": {
                         "branch": "master",
-                        "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5",
+                        "git_sha": "2be41ca2cc780eca4293d1b0dd3850b0b7ac40a3",
                         "installed_by": ["subworkflows"]
                     },
                     "fastq_download_prefetch_fasterqdump_sratools": {
diff --git a/modules/nf-core/seqkit/seq/environment.yml b/modules/nf-core/seqkit/seq/environment.yml
index 9019d269..74e0dd76 100644
--- a/modules/nf-core/seqkit/seq/environment.yml
+++ b/modules/nf-core/seqkit/seq/environment.yml
@@ -6,4 +6,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - "bioconda::seqkit=2.6.1"
+  - "bioconda::seqkit=2.8.1"
diff --git a/modules/nf-core/seqkit/seq/main.nf b/modules/nf-core/seqkit/seq/main.nf
index 7fc742b3..d7d38fc8 100644
--- a/modules/nf-core/seqkit/seq/main.nf
+++ b/modules/nf-core/seqkit/seq/main.nf
@@ -5,8 +5,8 @@ process SEQKIT_SEQ {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0':
-        'biocontainers/seqkit:2.6.1--h9ee0642_0' }"
+        'https://depot.galaxyproject.org/singularity/seqkit:2.8.1--h9ee0642_0':
+        'biocontainers/seqkit:2.8.1--h9ee0642_0' }"
 
     input:
     tuple val(meta), path(fastx)
diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test b/modules/nf-core/seqkit/seq/tests/main.nf.test
index aa9c283e..3ed23945 100644
--- a/modules/nf-core/seqkit/seq/tests/main.nf.test
+++ b/modules/nf-core/seqkit/seq/tests/main.nf.test
@@ -26,7 +26,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
@@ -48,7 +47,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
@@ -70,7 +68,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
@@ -116,7 +113,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test.snap b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap
index 91b4d17a..e6910966 100644
--- a/modules/nf-core/seqkit/seq/tests/main.nf.test.snap
+++ b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap
@@ -11,7 +11,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ],
                 "fastx": [
                     [
@@ -22,19 +22,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:34:00.37449"
-    },
-    "versions": {
-        "content": [
-            [
-                "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
-            ]
-        ],
-        "timestamp": "2023-12-17T13:56:53.318962"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:52:18.220051903"
     },
     "sarscov2-test_1_fastq_gz": {
         "content": [
@@ -48,7 +44,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ],
                 "fastx": [
                     [
@@ -59,11 +55,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:33:53.528342"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:51:55.607826581"
     },
     "sarscov2-genome_fasta": {
         "content": [
@@ -77,7 +77,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ],
                 "fastx": [
                     [
@@ -88,11 +88,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:33:44.757686"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:51:27.717072933"
     },
     "sarscov2-genome_fasta_gz": {
         "content": [
@@ -106,7 +110,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ],
                 "fastx": [
                     [
@@ -117,10 +121,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f"
+                    "versions.yml:md5,34894c4efa5e10a923e78975a3d260dd"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:33:49.115171"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:51:37.917560104"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/seqkit/sort/environment.yml b/modules/nf-core/seqkit/sort/environment.yml
index 820c5707..3a02d156 100644
--- a/modules/nf-core/seqkit/sort/environment.yml
+++ b/modules/nf-core/seqkit/sort/environment.yml
@@ -6,4 +6,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - "bioconda::seqkit=2.6.1"
+  - "bioconda::seqkit=2.8.1"
diff --git a/modules/nf-core/seqkit/sort/main.nf b/modules/nf-core/seqkit/sort/main.nf
index 08f86eb1..c12e35ac 100644
--- a/modules/nf-core/seqkit/sort/main.nf
+++ b/modules/nf-core/seqkit/sort/main.nf
@@ -5,8 +5,8 @@ process SEQKIT_SORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0':
-        'biocontainers/seqkit:2.6.1--h9ee0642_0' }"
+        'https://depot.galaxyproject.org/singularity/seqkit:2.8.1--h9ee0642_0':
+        'biocontainers/seqkit:2.8.1--h9ee0642_0' }"
 
     input:
     tuple val(meta), path(fastx)
diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test b/modules/nf-core/seqkit/sort/tests/main.nf.test
index 0c2f4e2d..e6f143dd 100644
--- a/modules/nf-core/seqkit/sort/tests/main.nf.test
+++ b/modules/nf-core/seqkit/sort/tests/main.nf.test
@@ -26,7 +26,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
@@ -48,7 +47,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
@@ -70,7 +68,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
@@ -116,7 +113,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test.snap b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap
index 94e2cb35..4d3a862b 100644
--- a/modules/nf-core/seqkit/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap
@@ -11,7 +11,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ],
                 "fastx": [
                     [
@@ -22,19 +22,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:07:19.28815"
-    },
-    "versions": {
-        "content": [
-            [
-                "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
-            ]
-        ],
-        "timestamp": "2023-12-18T10:07:15.341516"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T10:15:12.840547526"
     },
     "sarscov2-test_1_fastq_gz": {
         "content": [
@@ -48,7 +44,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ],
                 "fastx": [
                     [
@@ -59,11 +55,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:12:25.704893"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T10:14:53.190928013"
     },
     "sarscov2-genome_fasta": {
         "content": [
@@ -77,7 +77,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ],
                 "fastx": [
                     [
@@ -88,11 +88,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:07:15.293713"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T10:14:29.745582222"
     },
     "sarscov2-genome_fasta_gz": {
         "content": [
@@ -106,7 +110,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ],
                 "fastx": [
                     [
@@ -117,10 +121,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998"
+                    "versions.yml:md5,04ac93fea9e0019536cfe1445ef15472"
                 ]
             }
         ],
-        "timestamp": "2023-12-18T10:09:20.306713"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T10:14:41.460601093"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap
index 6e4e99c6..40bd469c 100644
--- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap
@@ -56,12 +56,12 @@
                 ],
                 "5": [
                     "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420",
-                    "versions.yml:md5,506585e66b23f17620bf582ef60af56d",
                     "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656",
                     "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f",
+                    "versions.yml:md5,702fdfb5f74070b208b10384021f3f28",
                     "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7",
-                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1",
-                    "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04"
+                    "versions.yml:md5,95dde1750b4171bedad83c5340cc712b",
+                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1"
                 ],
                 "aposteriori_sequence": [
                     [
@@ -117,16 +117,20 @@
                 ],
                 "versions": [
                     "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420",
-                    "versions.yml:md5,506585e66b23f17620bf582ef60af56d",
                     "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656",
                     "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f",
+                    "versions.yml:md5,702fdfb5f74070b208b10384021f3f28",
                     "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7",
-                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1",
-                    "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04"
+                    "versions.yml:md5,95dde1750b4171bedad83c5340cc712b",
+                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1"
                 ]
             }
         ],
-        "timestamp": "2023-12-12T15:33:35.106116"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:52:52.0126754"
     },
     "homo_sapiens-genome_fasta-genome_21_fasta-no_apriori-stub": {
         "content": [
@@ -181,10 +185,10 @@
                 ],
                 "5": [
                     "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420",
-                    "versions.yml:md5,506585e66b23f17620bf582ef60af56d",
                     "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656",
                     "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f",
-                    "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04"
+                    "versions.yml:md5,702fdfb5f74070b208b10384021f3f28",
+                    "versions.yml:md5,95dde1750b4171bedad83c5340cc712b"
                 ],
                 "aposteriori_sequence": [
                     [
@@ -236,14 +240,18 @@
                 ],
                 "versions": [
                     "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420",
-                    "versions.yml:md5,506585e66b23f17620bf582ef60af56d",
                     "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656",
                     "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f",
-                    "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04"
+                    "versions.yml:md5,702fdfb5f74070b208b10384021f3f28",
+                    "versions.yml:md5,95dde1750b4171bedad83c5340cc712b"
                 ]
             }
         ],
-        "timestamp": "2023-12-21T11:15:42.277945"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:53:21.817193592"
     },
     "homo_sapiens-genome_fasta-genome_21_fasta-partial_apriori-stub": {
         "content": [
@@ -308,12 +316,12 @@
                 ],
                 "5": [
                     "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420",
-                    "versions.yml:md5,506585e66b23f17620bf582ef60af56d",
                     "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656",
                     "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f",
+                    "versions.yml:md5,702fdfb5f74070b208b10384021f3f28",
                     "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7",
-                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1",
-                    "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04"
+                    "versions.yml:md5,95dde1750b4171bedad83c5340cc712b",
+                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1"
                 ],
                 "aposteriori_sequence": [
                     [
@@ -375,15 +383,19 @@
                 ],
                 "versions": [
                     "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420",
-                    "versions.yml:md5,506585e66b23f17620bf582ef60af56d",
                     "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656",
                     "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f",
+                    "versions.yml:md5,702fdfb5f74070b208b10384021f3f28",
                     "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7",
-                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1",
-                    "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04"
+                    "versions.yml:md5,95dde1750b4171bedad83c5340cc712b",
+                    "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1"
                 ]
             }
         ],
-        "timestamp": "2023-12-21T11:15:25.633714"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T08:53:07.119615485"
     }
 }
\ No newline at end of file

From 22ca108fe792015cb1374c41b868c92854aff48e Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 16 May 2024 14:46:43 +1200
Subject: [PATCH 39/76] Replaced GFF3_VALIDATE sub-workflow with
 GFF3_GT_GFF3_GFF3VALIDATOR_STAT

---
 CHANGELOG.md                                  |   1 +
 conf/modules.config                           |  42 +--
 modules.json                                  |  24 +-
 .../environment.yml                           |   9 -
 .../checkgff3fastacorrespondence/main.nf      |  25 --
 .../check_gff3_fasta_correspondence.sh        |  70 -----
 .../tests/main.nf.test                        |  70 -----
 .../tests/main.nf.test.snap                   |  72 -----
 .../tests/tags.yml                            |   2 -
 modules/pfr/gt/gff3/main.nf                   |   2 +-
 modules/pfr/gt/gff3/tests/main.nf.test        |   2 +-
 modules/pfr/gt/gff3/tests/main.nf.test.snap   |   4 +-
 modules/pfr/gt/gff3/tests/tags.yml            |   2 +-
 modules/pfr/gt/gff3validator/main.nf          |  29 +-
 .../pfr/gt/gff3validator/tests/main.nf.test   |  61 ++++-
 .../gt/gff3validator/tests/main.nf.test.snap  |  39 ++-
 .../gt/gff3validator/tests/nextflow.config    |   5 +
 modules/pfr/gt/gff3validator/tests/tags.yml   |   2 +-
 modules/pfr/samtools/faidx/environment.yml    |  10 +
 modules/pfr/samtools/faidx/main.nf            |  50 ++++
 .../faidx}/meta.yml                           |  61 +++--
 modules/pfr/samtools/faidx/tests/main.nf.test | 122 +++++++++
 .../samtools/faidx/tests/main.nf.test.snap    | 249 ++++++++++++++++++
 .../pfr/samtools/faidx/tests/nextflow.config  |   7 +
 .../pfr/samtools/faidx/tests/nextflow2.config |   6 +
 modules/pfr/samtools/faidx/tests/tags.yml     |   2 +
 .../gff3_gt_gff3_gff3validator_stat/main.nf   | 155 +++++++++++
 .../meta.yml                                  |  15 +-
 .../tests/main.nf.test                        |  53 +++-
 .../tests/main.nf.test.snap                   | 144 ++++++++++
 .../tests/nextflow.config                     |   0
 .../tests/tags.yml                            |   2 +
 subworkflows/pfr/gff3_validate/main.nf        |  61 -----
 .../pfr/gff3_validate/tests/main.nf.test.snap | 115 --------
 subworkflows/pfr/gff3_validate/tests/tags.yml |   2 -
 workflows/assemblyqc.nf                       |  20 +-
 36 files changed, 994 insertions(+), 541 deletions(-)
 delete mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/environment.yml
 delete mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/main.nf
 delete mode 100755 modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh
 delete mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test
 delete mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap
 delete mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml
 create mode 100644 modules/pfr/gt/gff3validator/tests/nextflow.config
 create mode 100644 modules/pfr/samtools/faidx/environment.yml
 create mode 100644 modules/pfr/samtools/faidx/main.nf
 rename modules/pfr/{custom/checkgff3fastacorrespondence => samtools/faidx}/meta.yml (51%)
 create mode 100644 modules/pfr/samtools/faidx/tests/main.nf.test
 create mode 100644 modules/pfr/samtools/faidx/tests/main.nf.test.snap
 create mode 100644 modules/pfr/samtools/faidx/tests/nextflow.config
 create mode 100644 modules/pfr/samtools/faidx/tests/nextflow2.config
 create mode 100644 modules/pfr/samtools/faidx/tests/tags.yml
 create mode 100644 subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main.nf
 rename subworkflows/pfr/{gff3_validate => gff3_gt_gff3_gff3validator_stat}/meta.yml (80%)
 rename subworkflows/pfr/{gff3_validate => gff3_gt_gff3_gff3validator_stat}/tests/main.nf.test (56%)
 create mode 100644 subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap
 rename subworkflows/pfr/{gff3_validate => gff3_gt_gff3_gff3validator_stat}/tests/nextflow.config (100%)
 create mode 100644 subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/tags.yml
 delete mode 100644 subworkflows/pfr/gff3_validate/main.nf
 delete mode 100644 subworkflows/pfr/gff3_validate/tests/main.nf.test.snap
 delete mode 100644 subworkflows/pfr/gff3_validate/tests/tags.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 90c44b94..45250fba 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -20,6 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 11. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
 12. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
 13. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
+14. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index 29f2e933..0359b8a1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -12,6 +12,28 @@
 
 process {
 
+    withName: '.*:GFF3_GT_GFF3_GFF3VALIDATOR_STAT:GT_GFF3' {
+        ext.args = '-tidy -retainids'
+    }
+
+    withName: '.*:GFF3_GT_GFF3_GFF3VALIDATOR_STAT:GT_STAT' {
+        ext.args = [
+            '-genelengthdistri',
+            '-genescoredistri',
+            '-exonlengthdistri',
+            '-exonnumberdistri',
+            '-intronlengthdistri',
+            '-cdslengthdistri',
+            '-addintrons'
+        ].join(' ').trim()
+
+        publishDir = [
+            path: { "${params.outdir}/genometools_gt_stat" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
+        ]
+    }
+
     withName: ASSEMBLATHON_STATS {
         publishDir = [
             path: { "${params.outdir}/assemblathon_stats" },
@@ -92,27 +114,7 @@ process {
         ]
     }
 
-    withName: '.*:GFF3_VALIDATE:GT_GFF3' {
-        ext.args = '-tidy -retainids'
-    }
 
-    withName: GT_STAT {
-        ext.args = [
-            '-genelengthdistri',
-            '-genescoredistri',
-            '-exonlengthdistri',
-            '-exonnumberdistri',
-            '-intronlengthdistri',
-            '-cdslengthdistri',
-            '-addintrons'
-        ].join(' ').trim()
-
-        publishDir = [
-            path: { "${params.outdir}/genometools_gt_stat" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
-        ]
-    }
 
     withName: FILTER_BY_LENGTH {
         ext.args = params.tidk_filter_by_size ? "-m ${params.tidk_filter_size_bp}" : ''
diff --git a/modules.json b/modules.json
index 59b548a7..f8bc841f 100644
--- a/modules.json
+++ b/modules.json
@@ -20,11 +20,6 @@
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
-                    "custom/checkgff3fastacorrespondence": {
-                        "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["gff3_validate"]
-                    },
                     "custom/restoregffids": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
@@ -37,18 +32,18 @@
                     },
                     "gt/gff3": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["gff3_validate"]
+                        "git_sha": "84f31d4be03e2ae6bee8e0b9fdea466be8e997bb",
+                        "installed_by": ["gff3_gt_gff3_gff3validator_stat"]
                     },
                     "gt/gff3validator": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["gff3_validate"]
+                        "git_sha": "84f31d4be03e2ae6bee8e0b9fdea466be8e997bb",
+                        "installed_by": ["gff3_gt_gff3_gff3validator_stat"]
                     },
                     "gt/stat": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
-                        "installed_by": ["modules"]
+                        "installed_by": ["gff3_gt_gff3_gff3validator_stat", "modules"]
                     },
                     "ltrfinder": {
                         "branch": "main",
@@ -74,6 +69,11 @@
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
+                    },
+                    "samtools/faidx": {
+                        "branch": "main",
+                        "git_sha": "84f31d4be03e2ae6bee8e0b9fdea466be8e997bb",
+                        "installed_by": ["gff3_gt_gff3_gff3validator_stat"]
                     }
                 }
             },
@@ -89,9 +89,9 @@
                         "git_sha": "258dbf212876e040e0049809ff0f9fc3f032b8d9",
                         "installed_by": ["subworkflows"]
                     },
-                    "gff3_validate": {
+                    "gff3_gt_gff3_gff3validator_stat": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "84f31d4be03e2ae6bee8e0b9fdea466be8e997bb",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml b/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml
deleted file mode 100644
index ec0e86d1..00000000
--- a/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml
+++ /dev/null
@@ -1,9 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-name: "custom_checkgff3fastacorrespondence"
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - "bioconda::samtools=1.18"
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/main.nf b/modules/pfr/custom/checkgff3fastacorrespondence/main.nf
deleted file mode 100644
index c1abb6f4..00000000
--- a/modules/pfr/custom/checkgff3fastacorrespondence/main.nf
+++ /dev/null
@@ -1,25 +0,0 @@
-process CUSTOM_CHECKGFF3FASTACORRESPONDENCE {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1':
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
-
-    input:
-    tuple val(meta), path(gff3)
-    path(fasta)
-
-    output:
-    tuple val(meta), path('*.success.log')  , emit: success_log , optional: true
-    tuple val(meta), path('*.error.log')    , emit: error_log   , optional: true
-    path "versions.yml"                     , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    shell:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    template 'check_gff3_fasta_correspondence.sh'
-}
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh b/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh
deleted file mode 100755
index 611c64b3..00000000
--- a/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh
+++ /dev/null
@@ -1,70 +0,0 @@
-#!/usr/bin/env bash
-
-# Bump VERSION on edit
-VERSION="v1"
-
-gff3_file="!{gff3}"
-fasta_file="!{fasta}"
-out_prefix="!{prefix}"
-task_process="!{task.process}"
-
-# Record versions
-cat <<-END_VERSIONS > versions.yml
-"${task_process}":
-    samtools: $(echo $(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*$//' )
-END_VERSIONS
-
-# Requires
-# samtools faidx
-
-## STEP 1
-# Check that gff3 has no identifers that are not in fasta (fasta can
-# have ids that are not in gff3 since not all assembly units have gff3 records
-
-# Extract identifiers from the GFF3 file
-gff3_identifiers=$(grep -v '^#' "$gff3_file" | awk '{print $1}' | sort -u)
-
-# Extract identifiers from the FASTA file
-fasta_identifiers=$(grep '^>' "$fasta_file" | awk '{print substr($1, 2)}' | sort -u)
-
-# Compare identifiers and find any that are present in the GFF3 but not in the FASTA
-missing_identifiers=$(comm -23 <(echo "$gff3_identifiers") <(echo "$fasta_identifiers"))
-
-# Check if any missing identifiers were found
-if [[ -n "$missing_identifiers" ]]; then
-    touch "${out_prefix}.error.log"
-    echo "Failed to validate gff3 file for: $tag_label"         >> "${out_prefix}.error.log"
-    echo "Fasta file: $fasta_file"                              >> "${out_prefix}.error.log"
-    echo "Gff3 file: $gff3_file"                                >> "${out_prefix}.error.log"
-    echo "GFF3 file contains identifiers not present in FASTA:" >> "${out_prefix}.error.log"
-    echo "$missing_identifiers"                                 >> "${out_prefix}.error.log"
-    exit 0
-fi
-
-## STEP 2
-# check that there are no coordiantes in gff3 for any seqid that are
-# greater than the seq length of the paretn fasta entry
-
-# Compute sequence lengths using samtools faidx
-samtools faidx "$fasta_file" | cut -f 1,2 > sequence_lengths.txt
-
-# Check GFF3 file for coordinates exceeding sequence lengths
-while IFS=$'\t' read -r seqname source feature start end score strand frame attributes && \
-    read -r seq seq_length <&3; do
-    if [[ $start -gt $seq_length || $end -gt $seq_length ]]; then
-        touch "${out_prefix}.error.log"
-        echo "Failed to validate gff3 file for: $tag_label"     >> "${out_prefix}.error.log"
-        echo "Fasta file: $fasta_file"                          >> "${out_prefix}.error.log"
-        echo "Gff3 file: $gff3_file"                            >> "${out_prefix}.error.log"
-        echo "Coordinates exceed sequence length in GFF3 file:" >> "${out_prefix}.error.log"
-        echo "Sequence: $seqname"                               >> "${out_prefix}.error.log"
-        echo "Sequence length: $seq_length"                     >> "${out_prefix}.error.log"
-        echo "Start: $start"                                    >> "${out_prefix}.error.log"
-        echo "End: $end"                                        >> "${out_prefix}.error.log"
-        exit 0
-    fi
-done < "$gff3_file" 3< "sequence_lengths.txt"
-
-touch "${out_prefix}.success.log"
-echo "All tests passed..."                                      >>  "${out_prefix}.success.log"
-exit 0
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test
deleted file mode 100644
index a45992c2..00000000
--- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test
+++ /dev/null
@@ -1,70 +0,0 @@
-nextflow_process {
-
-    name "Test Process CUSTOM_CHECKGFF3FASTACORRESPONDENCE"
-    script "../main.nf"
-    process "CUSTOM_CHECKGFF3FASTACORRESPONDENCE"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "custom"
-    tag "custom/checkgff3fastacorrespondence"
-
-    test("sarscov2-fasta-gff3-success") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
-                ]
-
-                input[1] = [
-                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert process.out.error_log == [] },
-                { assert process.out.success_log != null },
-                { assert path(process.out.success_log.get(0).get(1)).getText().contains("All tests passed...")},
-            )
-        }
-
-    }
-
-    test("sarscov2-gff3-homo_sapiens-fasta-error") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
-                ]
-
-                input[1] = [
-                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert process.out.success_log == [] },
-                { assert process.out.error_log != null },
-                { assert path(process.out.error_log.get(0).get(1)).getText().contains("GFF3 file contains identifiers not present in FASTA")},
-            )
-        }
-
-    }
-
-}
\ No newline at end of file
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap
deleted file mode 100644
index 261e0dc3..00000000
--- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap
+++ /dev/null
@@ -1,72 +0,0 @@
-{
-    "sarscov2-gff3-homo_sapiens-fasta-error": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802"
-                ],
-                "error_log": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f"
-                    ]
-                ],
-                "success_log": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802"
-                ]
-            }
-        ],
-        "timestamp": "2023-11-29T12:24:08.677505"
-    },
-    "sarscov2-fasta-gff3-success": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802"
-                ],
-                "error_log": [
-                    
-                ],
-                "success_log": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802"
-                ]
-            }
-        ],
-        "timestamp": "2023-11-29T12:24:04.530428"
-    }
-}
\ No newline at end of file
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml b/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml
deleted file mode 100644
index 708130d8..00000000
--- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-custom/checkgff3fastacorrespondence:
-  - "modules/pfr/custom/checkgff3fastacorrespondence/**"
diff --git a/modules/pfr/gt/gff3/main.nf b/modules/pfr/gt/gff3/main.nf
index d27e2bb9..6324a391 100644
--- a/modules/pfr/gt/gff3/main.nf
+++ b/modules/pfr/gt/gff3/main.nf
@@ -27,7 +27,7 @@ process GT_GFF3 {
         $args \\
         "$gff3" \\
         > "${prefix}.gt.gff3" \\
-        2> "${prefix}.error.log" \\
+        2> >(tee "${prefix}.error.log" >&2) \\
         || echo "Errors from gt-gff3 printed to ${prefix}.error.log"
 
     if grep -q "gt gff3: error:" "${prefix}.error.log"; then
diff --git a/modules/pfr/gt/gff3/tests/main.nf.test b/modules/pfr/gt/gff3/tests/main.nf.test
index b57439ef..cb44bc8f 100644
--- a/modules/pfr/gt/gff3/tests/main.nf.test
+++ b/modules/pfr/gt/gff3/tests/main.nf.test
@@ -58,4 +58,4 @@ nextflow_process {
         }
     }
 
-}
\ No newline at end of file
+}
diff --git a/modules/pfr/gt/gff3/tests/main.nf.test.snap b/modules/pfr/gt/gff3/tests/main.nf.test.snap
index f31e8d1c..6e454f18 100644
--- a/modules/pfr/gt/gff3/tests/main.nf.test.snap
+++ b/modules/pfr/gt/gff3/tests/main.nf.test.snap
@@ -32,7 +32,7 @@
                 ]
             }
         ],
-        "timestamp": "2023-11-28T13:43:34.620429"
+        "timestamp": "2023-11-29T10:42:11.408352"
     },
     "sarscov2-gff3-valid": {
         "content": [
@@ -67,6 +67,6 @@
                 ]
             }
         ],
-        "timestamp": "2023-11-28T13:43:31.065832"
+        "timestamp": "2023-11-29T10:42:07.817894"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/gt/gff3/tests/tags.yml b/modules/pfr/gt/gff3/tests/tags.yml
index ae040309..0ce15a90 100644
--- a/modules/pfr/gt/gff3/tests/tags.yml
+++ b/modules/pfr/gt/gff3/tests/tags.yml
@@ -1,2 +1,2 @@
 gt/gff3:
-  - "modules/pfr/gt/gff3/**"
+  - "modules/nf-core/gt/gff3/**"
diff --git a/modules/pfr/gt/gff3validator/main.nf b/modules/pfr/gt/gff3validator/main.nf
index ae7ec9e7..8c6899ca 100644
--- a/modules/pfr/gt/gff3validator/main.nf
+++ b/modules/pfr/gt/gff3validator/main.nf
@@ -24,22 +24,22 @@ process GT_GFF3VALIDATOR {
     gt \\
         gff3validator \\
         "$gff3" \\
-        > "${prefix}.success.log" \\
-        2> "${prefix}.error.log" \\
+        > "${prefix}.stdout" \\
+        2> >(tee "${prefix}.stderr" >&2) \\
         || echo "Errors from gt-gff3validator printed to ${prefix}.error.log"
 
-    if grep -q "input is valid GFF3" "${prefix}.success.log"; then
+    if grep -q "input is valid GFF3" "${prefix}.stdout"; then
         echo "Validation successful..."
-
+        # emit stdout to the success output channel
         mv \\
-            "${prefix}.error.log" \\
-            gt_gff3validator.stderr
+            "${prefix}.stdout" \\
+            "${prefix}.success.log"
     else
         echo "Validation failed..."
-
+        # emit stderr to the error output channel
         mv \\
-            "${prefix}.success.log" \\
-            gt_gff3validator.stdout
+            "${prefix}.stderr" \\
+            "${prefix}.error.log"
     fi
 
     cat <<-END_VERSIONS > versions.yml
@@ -47,4 +47,15 @@ process GT_GFF3VALIDATOR {
         genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch "${prefix}.success.log"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //')
+    END_VERSIONS
+    """
 }
diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test b/modules/pfr/gt/gff3validator/tests/main.nf.test
index 9addd7d8..766e3e74 100644
--- a/modules/pfr/gt/gff3validator/tests/main.nf.test
+++ b/modules/pfr/gt/gff3validator/tests/main.nf.test
@@ -8,18 +8,31 @@ nextflow_process {
     tag "modules_nfcore"
     tag "gt"
     tag "gt/gff3validator"
+    tag "gt/gff3"
 
-    test("custom-gff3-valid") {
+    test("sarscov2-gff3-valid") {
+
+        config './nextflow.config'
+
+        setup {
+            run("GT_GFF3") {
+                script "../../../../nf-core/gt/gff3"
+
+                process {
+                    """
+                    input[0] = [
+                        [ id:'test' ],
+                        file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                    ]
+                    """
+                }
+            }
+        }
 
         when {
             process {
                 """
-                input[0] = Channel.of(
-                        '##gff-version 3',
-                        'chr22\thavana\tpseudogene\t16572027\t16574637\t.\t+\t.\tID=gene:ENSG00000233995;Name=AP000547.1'
-                    )
-                    .collectFile(name: 'sample.gff3', newLine: true)
-                    .map { file -> [ [ id:'test' ], file ] }
+                input[0] = GT_GFF3.out.gt_gff3
                 """
             }
         }
@@ -28,9 +41,32 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert process.out.error_log == [] },
-                { assert process.out.success_log != null },
-                { assert path(process.out.success_log.get(0).get(1)).getText().contains("input is valid GFF3") }
+                { assert path(process.out.success_log[0][1]).text.contains("input is valid GFF3") },
+                { assert process.out.error_log == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
             )
         }
 
@@ -54,10 +90,9 @@ nextflow_process {
                 { assert process.success },
                 { assert snapshot(process.out).match() },
                 { assert process.out.success_log == [] },
-                { assert process.out.error_log != null },
-                { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3validator: error:") }
+                { assert path(process.out.error_log[0][1]).text.contains("gt gff3validator: error:") }
             )
         }
 
     }
-}
\ No newline at end of file
+}
diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test.snap b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap
index 0b6f065a..ccc83f90 100644
--- a/modules/pfr/gt/gff3validator/tests/main.nf.test.snap
+++ b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap
@@ -1,4 +1,39 @@
 {
+    "sarscov2-gff3-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.success.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,5927673eb73a8c22408643d224414215"
+                ],
+                "error_log": [
+                    
+                ],
+                "success_log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.success.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5927673eb73a8c22408643d224414215"
+                ]
+            }
+        ],
+        "timestamp": "2024-01-11T09:29:13.14468"
+    },
     "sarscov2-gff3-invalid": {
         "content": [
             {
@@ -34,7 +69,7 @@
         ],
         "timestamp": "2023-11-29T11:09:23.708792"
     },
-    "custom-gff3-valid": {
+    "sarscov2-gff3-valid": {
         "content": [
             {
                 "0": [
@@ -67,6 +102,6 @@
                 ]
             }
         ],
-        "timestamp": "2023-11-29T11:09:19.530068"
+        "timestamp": "2023-12-13T10:34:54.485391"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/gt/gff3validator/tests/nextflow.config b/modules/pfr/gt/gff3validator/tests/nextflow.config
new file mode 100644
index 00000000..c69915da
--- /dev/null
+++ b/modules/pfr/gt/gff3validator/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: GT_GFF3 {
+        ext.args = '-tidy -retainids'
+    }
+}
diff --git a/modules/pfr/gt/gff3validator/tests/tags.yml b/modules/pfr/gt/gff3validator/tests/tags.yml
index e247d55e..98c39ffe 100644
--- a/modules/pfr/gt/gff3validator/tests/tags.yml
+++ b/modules/pfr/gt/gff3validator/tests/tags.yml
@@ -1,2 +1,2 @@
 gt/gff3validator:
-  - "modules/pfr/gt/gff3validator/**"
+  - "modules/nf-core/gt/gff3validator/**"
diff --git a/modules/pfr/samtools/faidx/environment.yml b/modules/pfr/samtools/faidx/environment.yml
new file mode 100644
index 00000000..9c24eb0a
--- /dev/null
+++ b/modules/pfr/samtools/faidx/environment.yml
@@ -0,0 +1,10 @@
+name: samtools_faidx
+
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+
+dependencies:
+  - bioconda::htslib=1.19.1
+  - bioconda::samtools=1.19.2
diff --git a/modules/pfr/samtools/faidx/main.nf b/modules/pfr/samtools/faidx/main.nf
new file mode 100644
index 00000000..cfe7ad95
--- /dev/null
+++ b/modules/pfr/samtools/faidx/main.nf
@@ -0,0 +1,50 @@
+process SAMTOOLS_FAIDX {
+    tag "$fasta"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
+
+    input:
+    tuple val(meta), path(fasta)
+    tuple val(meta2), path(fai)
+
+    output:
+    tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true
+    tuple val(meta), path ("*.fai")        , emit: fai, optional: true
+    tuple val(meta), path ("*.gzi")        , emit: gzi, optional: true
+    path "versions.yml"                    , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    samtools \\
+        faidx \\
+        $fasta \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll()
+    def fastacmd = match[0] ? "touch ${match[0][1]}" : ''
+    """
+    ${fastacmd}
+    touch ${fasta}.fai
+
+    cat <<-END_VERSIONS > versions.yml
+
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml b/modules/pfr/samtools/faidx/meta.yml
similarity index 51%
rename from modules/pfr/custom/checkgff3fastacorrespondence/meta.yml
rename to modules/pfr/samtools/faidx/meta.yml
index 69bbd053..f3c25de2 100644
--- a/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml
+++ b/modules/pfr/samtools/faidx/meta.yml
@@ -1,12 +1,9 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
-name: "custom_checkgff3fastacorrespondence"
-description: "A custom bash script which checks the correspondence of a gff3 file with a fasta file"
+name: samtools_faidx
+description: Index FASTA file
 keywords:
-  - genome
-  - gff3
-  - annotation
-  - validation
+  - index
+  - fasta
+  - faidx
 tools:
   - samtools:
       description: |
@@ -17,40 +14,52 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
-
 input:
   - meta:
       type: map
       description: |
-        Groovy Map containing sample information
-        e.g. `[ id:'test' ]`
-  - gff3:
-      type: file
-      description: Input gff3 file
-      pattern: "*.{gff,gff3}"
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
   - fasta:
       type: file
-      description: Input fasta file
-      pattern: "*.{fsa,fa,fasta}"
+      description: FASTA file
+      pattern: "*.{fa,fasta}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - fai:
+      type: file
+      description: FASTA index file
+      pattern: "*.{fai}"
 output:
   - meta:
       type: map
       description: |
         Groovy Map containing sample information
-        e.g. `[ id:'test' ]`
-  - success_log:
+        e.g. [ id:'test', single_end:false ]
+  - fa:
+      type: file
+      description: FASTA file
+      pattern: "*.{fa}"
+  - fai:
       type: file
-      description: Log file for successful validation
-      pattern: "*.success.log"
-  - error_log:
+      description: FASTA index file
+      pattern: "*.{fai}"
+  - gzi:
       type: file
-      description: Log file for failed validation
-      pattern: "*.error.log"
+      description: Optional gzip index file for compressed inputs
+      pattern: "*.gzi"
   - versions:
       type: file
       description: File containing software versions
       pattern: "versions.yml"
 authors:
-  - "@GallVp"
+  - "@drpatelh"
+  - "@ewels"
+  - "@phue"
 maintainers:
-  - "@GallVp"
+  - "@drpatelh"
+  - "@ewels"
+  - "@phue"
diff --git a/modules/pfr/samtools/faidx/tests/main.nf.test b/modules/pfr/samtools/faidx/tests/main.nf.test
new file mode 100644
index 00000000..17244ef2
--- /dev/null
+++ b/modules/pfr/samtools/faidx/tests/main.nf.test
@@ -0,0 +1,122 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_FAIDX"
+    script "../main.nf"
+    process "SAMTOOLS_FAIDX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/faidx"
+
+    test("test_samtools_faidx") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_bgzip") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)]
+
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_fasta") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_stub_fasta") {
+
+        config "./nextflow2.config"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_stub_fai") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/samtools/faidx/tests/main.nf.test.snap b/modules/pfr/samtools/faidx/tests/main.nf.test.snap
new file mode 100644
index 00000000..3e651ef6
--- /dev/null
+++ b/modules/pfr/samtools/faidx/tests/main.nf.test.snap
@@ -0,0 +1,249 @@
+{
+    "test_samtools_faidx": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:22:39.412601"
+    },
+    "test_samtools_faidx_bgzip": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:23:22.427966"
+    },
+    "test_samtools_faidx_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36"
+                    ]
+                ],
+                "fai": [
+                    
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:24:04.107537"
+    },
+    "test_samtools_faidx_stub_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "fai": [
+                    
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:24:45.868463"
+    },
+    "test_samtools_faidx_stub_fai": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:25:27.550554"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/samtools/faidx/tests/nextflow.config b/modules/pfr/samtools/faidx/tests/nextflow.config
new file mode 100644
index 00000000..f76a3ba0
--- /dev/null
+++ b/modules/pfr/samtools/faidx/tests/nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: SAMTOOLS_FAIDX {
+        ext.args = 'MT192765.1 -o extract.fa'
+    }
+
+}
diff --git a/modules/pfr/samtools/faidx/tests/nextflow2.config b/modules/pfr/samtools/faidx/tests/nextflow2.config
new file mode 100644
index 00000000..33ebbd5d
--- /dev/null
+++ b/modules/pfr/samtools/faidx/tests/nextflow2.config
@@ -0,0 +1,6 @@
+process {
+
+    withName: SAMTOOLS_FAIDX {
+        ext.args = '-o extract.fa'
+    }
+}
diff --git a/modules/pfr/samtools/faidx/tests/tags.yml b/modules/pfr/samtools/faidx/tests/tags.yml
new file mode 100644
index 00000000..e4a83948
--- /dev/null
+++ b/modules/pfr/samtools/faidx/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/faidx:
+  - modules/nf-core/samtools/faidx/**
diff --git a/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main.nf b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main.nf
new file mode 100644
index 00000000..3891fc81
--- /dev/null
+++ b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main.nf
@@ -0,0 +1,155 @@
+include { GT_GFF3                               } from '../../../modules/pfr/gt/gff3/main'
+include { SAMTOOLS_FAIDX                        } from '../../../modules/pfr/samtools/faidx/main'
+include { GT_GFF3VALIDATOR                      } from '../../../modules/pfr/gt/gff3validator/main'
+include { GT_STAT                               } from '../../../modules/pfr/gt/stat/main'
+
+workflow GFF3_GT_GFF3_GFF3VALIDATOR_STAT {
+
+    take:
+    ch_gff3                                     // channel: [ val(meta), gff3 ]
+    ch_fasta                                    // channel: [ val(meta), fasta ]
+
+    main:
+
+    ch_versions                                 = Channel.empty()
+
+    // MODULE: GT_GFF3
+    GT_GFF3 ( ch_gff3 )
+
+    ch_versions                                 = ch_versions.mix(GT_GFF3.out.versions.first())
+
+    // MODULE: GT_GFF3VALIDATOR
+    GT_GFF3VALIDATOR ( GT_GFF3.out.gt_gff3 )
+
+    ch_versions                                 = ch_versions.mix(GT_GFF3VALIDATOR.out.versions.first())
+
+    // MODULE: SAMTOOLS_FAIDX
+    SAMTOOLS_FAIDX(
+        ch_fasta,
+        [ [], [] ]
+    )
+
+    ch_fai                                      = SAMTOOLS_FAIDX.out.fai
+    ch_versions                                 = ch_versions.mix(SAMTOOLS_FAIDX.out.versions.first())
+
+    // FUNCTION: checkGff3FastaCorrespondence
+    ch_gff3_fai                                 = GT_GFF3VALIDATOR.out.success_log
+                                                | join (
+                                                    GT_GFF3.out.gt_gff3
+                                                )
+                                                | map { meta, log, gff3 -> [ meta, gff3 ] }
+                                                | join (
+                                                    ch_fai
+                                                )
+
+    ch_correspondence_status                    = ch_gff3_fai
+                                                | map { meta, gff3, fai ->
+                                                    checkGff3FastaCorrespondence ( meta, gff3, fai )
+                                                }
+
+    ch_correspondence_success                   = ch_correspondence_status
+                                                | map { meta, success, error ->
+                                                    if ( success ) {
+                                                        [ meta, success ]
+                                                    }
+                                                }
+
+    ch_correspondence_error                     = ch_correspondence_status
+                                                | map { meta, success, error ->
+                                                    if ( error ) {
+                                                        [ "${meta.id}.error.log", error.join('\n') ]
+                                                    }
+                                                }
+                                                | collectFile
+                                                | map { error ->
+                                                    [ [ id: error.baseName.replace('.error', '') ], error ]
+                                                }
+
+    ch_valid_gff3                               = ch_correspondence_success
+                                                | join (
+                                                    ch_gff3_fai
+                                                    | map { meta, gff3, fai -> [ meta, gff3 ] }
+                                                )
+                                                | map { meta, log, gff3 -> [ meta, gff3 ] }
+
+    ch_log_for_invalid_gff3                     = GT_GFF3.out.error_log
+                                                | mix (
+                                                    GT_GFF3VALIDATOR.out.error_log
+                                                )
+                                                | mix (
+                                                    ch_correspondence_error
+                                                )
+
+    // MODULE: GT_STAT
+    GT_STAT ( ch_valid_gff3 )
+
+    ch_gff3_stats                               = GT_STAT.out.stats
+    ch_versions                                 = ch_versions.mix(GT_STAT.out.versions.first())
+
+    emit:
+    valid_gff3              = ch_valid_gff3             // channel: [ val(meta), gff3 ]
+    gff3_stats              = ch_gff3_stats             // channel: [ val(meta), yml ]
+    log_for_invalid_gff3    = ch_log_for_invalid_gff3   // channel: [ val(meta), log ]
+    versions                = ch_versions               // channel: [ versions.yml ]
+}
+
+def checkGff3FastaCorrespondence(meta, gff3File, faiFile) {
+
+    // STEP 1
+    // Check that gff3 has no identifiers that are not in fasta
+    def gff3Lines           = gff3File.readLines().findAll { ! it.startsWith('#') }
+    def gff3Identifiers     = gff3Lines.collect { it.split('\t')[0] }.unique().sort()
+    def fastaIdentifiers    = faiFile.readLines().collect { it.split('\t')[0] }.unique().sort()
+    def missingIdentifiers  = gff3Identifiers.findAll { ! fastaIdentifiers.contains(it) }
+
+    if (missingIdentifiers) {
+        return [
+            meta,
+            [], // success log
+            [
+                "Failed to validate gff3 file: ${gff3File.name}",
+                "GFF3 file contains identifiers not present in FASTA:",
+                "${missingIdentifiers.join('\n')}"
+            ] // error log
+        ]
+    }
+
+    // STEP 2
+    // Check that there are no coordinates in gff3 that exceed the sequence length in the parent fasta entry
+    def sequenceLengths = [:]
+    faiFile.readLines().each { line ->
+        def parts = line.split('\t')
+        sequenceLengths[parts[0]] = parts[1]
+    }
+
+    for ( line in gff3Lines ) {
+        def parts = line.split('\t')
+        def name = parts[0]
+        def start = parts[3].toInteger()
+        def end = parts[4].toInteger()
+        def seqLength = sequenceLengths[name].toInteger()
+
+        if (start > seqLength || end > seqLength) {
+            return [
+                meta,
+                [], // success log
+                [
+                    "Failed to validate gff3: ${gff3File.name}",
+                    "Coordinates exceed sequence length in GFF3 file:",
+                    "Sequence: $name",
+                    "Sequence length: $seqLength",
+                    "Start: $start",
+                    "End: $end"
+                ] // error log
+            ]
+        }
+    }
+
+    return [
+        meta,
+        [
+            "All tests passed..."
+        ], // success log
+        [] // error log
+    ]
+}
diff --git a/subworkflows/pfr/gff3_validate/meta.yml b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/meta.yml
similarity index 80%
rename from subworkflows/pfr/gff3_validate/meta.yml
rename to subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/meta.yml
index 5dea12a5..0d12d5fd 100644
--- a/subworkflows/pfr/gff3_validate/meta.yml
+++ b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/meta.yml
@@ -1,17 +1,18 @@
 # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
-name: gff3_validate
+name: gff3_gt_gff3_gff3validator_stat
 description: |
-  Validates a gff3 file using GenomeTools gt-gff3, gt-gff3validator and
-  checks its correspondence with a fasta file
+  Validates a gff3 file using GenomeTools gt-gff3, gt-gff3validator, checks its correspondence with a fasta file and produces stats with gt-stat
 keywords:
   - genome
   - gff3
   - annotation
   - validation
+  - stats
 components:
   - gt/gff3
   - gt/gff3validator
-  - custom/checkgff3fastacorrespondence
+  - samtools/faidx
+  - gt/stat
 input:
   - ch_gff3:
       type: file
@@ -32,6 +33,12 @@ output:
         Valid gff3 file
         Structure: [ val(meta), path(gff3) ]
       pattern: "*.gff3"
+  - gff3_stats:
+      type: file
+      description: |
+        Statistics
+        Structure: [ val(meta), path(yml) ]
+      pattern: "*.yml"
   - log_for_invalid_gff3:
       type: file
       description: |
diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test
similarity index 56%
rename from subworkflows/pfr/gff3_validate/tests/main.nf.test
rename to subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test
index 14c33ae2..f15972fa 100644
--- a/subworkflows/pfr/gff3_validate/tests/main.nf.test
+++ b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test
@@ -1,21 +1,22 @@
 nextflow_workflow {
 
-    name "Test Workflow GFF3_VALIDATE"
+    name "Test Workflow GFF3_GT_GFF3_GFF3VALIDATOR_STAT"
     script "../main.nf"
-    workflow "GFF3_VALIDATE"
+    workflow "GFF3_GT_GFF3_GFF3VALIDATOR_STAT"
     config "./nextflow.config"
 
     tag "subworkflows"
     tag "subworkflows_nfcore"
-    tag "subworkflows/gff3_validate"
-    tag "gff3_validate"
+    tag "subworkflows/gff3_gt_gff3_gff3validator_stat"
+    tag "gff3_gt_gff3_gff3validator_stat"
     tag "gt"
     tag "gt/gff3"
     tag "gt/gff3validator"
-    tag "custom"
-    tag "custom/checkgff3fastacorrespondence"
+    tag "gt/stat"
+    tag "samtools"
+    tag "samtools/faidx"
 
-    test("sarscov2-genome_gff3-genome_fasta-all_pass") {
+    test("sarscov2-genome_gff3-all_pass") {
 
         when {
             workflow {
@@ -38,7 +39,7 @@ nextflow_workflow {
         }
     }
 
-    test("homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail") {
+    test("homo_sapiens-genome_bed-gt_gff3_fail") {
 
         when {
             workflow {
@@ -79,7 +80,41 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success},
-                { assert snapshot(workflow.out).match()}
+                { assert snapshot(
+                    workflow.out.valid_gff3,
+                    workflow.out.versions).match()
+                },
+                { assert path(workflow.out.log_for_invalid_gff3[0][1]).text.contains('GFF3 file contains identifiers not present in FASTA') }
+            )
+        }
+    }
+
+    test("sarscov2-genome_gff3-test_fasta-correspondence_fail-out_of_bounds") {
+
+        when {
+            workflow {
+                """
+                def test_fasta = new File('genome.fasta')
+                test_fasta.text = '>MT192765.1\\nAGCTAGCT'
+
+                input[0] = Channel.of([ [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                ])
+                input[1] = Channel.of([ [ id:'test' ],
+                    test_fasta.toPath()
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    workflow.out.valid_gff3,
+                    workflow.out.versions).match()
+                },
+                { assert path(workflow.out.log_for_invalid_gff3[0][1]).text.contains('Coordinates exceed sequence length in GFF3 file') }
             )
         }
     }
diff --git a/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap
new file mode 100644
index 00000000..57aadb93
--- /dev/null
+++ b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap
@@ -0,0 +1,144 @@
+{
+    "homo_sapiens-genome_bed-gt_gff3_fail": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.error.log:md5,c096494c3cd02864eb54434c294ba382"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff",
+                    "versions.yml:md5,d81758cb7178ee32a1fb78f8639504d8"
+                ],
+                "gff3_stats": [
+                    
+                ],
+                "log_for_invalid_gff3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.error.log:md5,c096494c3cd02864eb54434c294ba382"
+                    ]
+                ],
+                "valid_gff3": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff",
+                    "versions.yml:md5,d81758cb7178ee32a1fb78f8639504d8"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T19:41:05.240982"
+    },
+    "sarscov2-genome_gff3-test_fasta-correspondence_fail-out_of_bounds": {
+        "content": [
+            [
+                
+            ],
+            [
+                "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff",
+                "versions.yml:md5,c89b081a13c68acc5326e43ca9104344",
+                "versions.yml:md5,d81758cb7178ee32a1fb78f8639504d8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T19:41:16.606045"
+    },
+    "sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail": {
+        "content": [
+            [
+                
+            ],
+            [
+                "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff",
+                "versions.yml:md5,c89b081a13c68acc5326e43ca9104344",
+                "versions.yml:md5,d81758cb7178ee32a1fb78f8639504d8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T19:41:11.28855"
+    },
+    "sarscov2-genome_gff3-all_pass": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.yml:md5,762d733791108d9723989c3bbc69fa2b"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff",
+                    "versions.yml:md5,80555fe6e28e9564cb534f5478842286",
+                    "versions.yml:md5,c89b081a13c68acc5326e43ca9104344",
+                    "versions.yml:md5,d81758cb7178ee32a1fb78f8639504d8"
+                ],
+                "gff3_stats": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.yml:md5,762d733791108d9723989c3bbc69fa2b"
+                    ]
+                ],
+                "log_for_invalid_gff3": [
+                    
+                ],
+                "valid_gff3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff",
+                    "versions.yml:md5,80555fe6e28e9564cb534f5478842286",
+                    "versions.yml:md5,c89b081a13c68acc5326e43ca9104344",
+                    "versions.yml:md5,d81758cb7178ee32a1fb78f8639504d8"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-10T09:03:38.938037"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/pfr/gff3_validate/tests/nextflow.config b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/nextflow.config
similarity index 100%
rename from subworkflows/pfr/gff3_validate/tests/nextflow.config
rename to subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/nextflow.config
diff --git a/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/tags.yml b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/tags.yml
new file mode 100644
index 00000000..2b0b9279
--- /dev/null
+++ b/subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/gff3_gt_gff3_gff3validator_stat:
+  - subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/**
diff --git a/subworkflows/pfr/gff3_validate/main.nf b/subworkflows/pfr/gff3_validate/main.nf
deleted file mode 100644
index 5437c5a6..00000000
--- a/subworkflows/pfr/gff3_validate/main.nf
+++ /dev/null
@@ -1,61 +0,0 @@
-include { GT_GFF3                               } from '../../../modules/pfr/gt/gff3/main'
-include { GT_GFF3VALIDATOR                      } from '../../../modules/pfr/gt/gff3validator/main'
-include { CUSTOM_CHECKGFF3FASTACORRESPONDENCE   } from '../../../modules/pfr/custom/checkgff3fastacorrespondence/main'
-
-workflow GFF3_VALIDATE {
-
-    take:
-    ch_gff3     // channel: [ val(meta), gff3 ]
-    ch_fasta    // channel: [ val(meta), fasta ]
-
-    main:
-
-    ch_versions = Channel.empty()
-
-    // MODULE: GT_GFF3
-    GT_GFF3 ( ch_gff3 )
-    ch_versions = ch_versions.mix(GT_GFF3.out.versions.first())
-
-    // MODULE: GT_GFF3VALIDATOR
-    GT_GFF3VALIDATOR ( GT_GFF3.out.gt_gff3 )
-    ch_versions = ch_versions.mix(GT_GFF3VALIDATOR.out.versions.first())
-
-    // MODULE: CUSTOM_CHECKGFF3FASTACORRESPONDENCE
-    GT_GFF3VALIDATOR.out.success_log
-    | join (
-        GT_GFF3.out.gt_gff3
-    )
-    | map { meta, log, gff3 -> [ meta, gff3 ] }
-    | join (
-        ch_fasta
-    )
-    | set { ch_gff3_fasta }
-
-    CUSTOM_CHECKGFF3FASTACORRESPONDENCE (
-        ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] },
-        ch_gff3_fasta.map { meta, gff3, fasta -> fasta }
-    )
-
-    ch_versions = ch_versions.mix(CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.versions.first())
-
-    CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.success_log
-    | join (
-        ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] }
-    )
-    | map { meta, log, gff3 -> [ meta, gff3 ] }
-    | set { ch_valid_gff3 }
-
-    GT_GFF3.out.error_log
-    | mix (
-        GT_GFF3VALIDATOR.out.error_log
-    )
-    | mix (
-        CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.error_log
-    )
-    | set { ch_log_for_invalid_gff3 }
-
-    emit:
-    valid_gff3              = ch_valid_gff3             // channel: [ val(meta), gff3 ]
-    log_for_invalid_gff3    = ch_log_for_invalid_gff3   // channel: [ val(meta), log ]
-    versions                = ch_versions               // channel: [ versions.yml ]
-}
diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap b/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap
deleted file mode 100644
index 4d2a59b2..00000000
--- a/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap
+++ /dev/null
@@ -1,115 +0,0 @@
-{
-    "sarscov2-genome_gff3-genome_fasta-all_pass": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34",
-                    "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd",
-                    "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae"
-                ],
-                "log_for_invalid_gff3": [
-                    
-                ],
-                "valid_gff3": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34",
-                    "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd",
-                    "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae"
-                ]
-            }
-        ],
-        "timestamp": "2023-12-07T10:33:21.09887"
-    },
-    "homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.error.log:md5,c096494c3cd02864eb54434c294ba382"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae"
-                ],
-                "log_for_invalid_gff3": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.error.log:md5,c096494c3cd02864eb54434c294ba382"
-                    ]
-                ],
-                "valid_gff3": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae"
-                ]
-            }
-        ],
-        "timestamp": "2023-12-07T10:35:26.549003"
-    },
-    "sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34",
-                    "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd",
-                    "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae"
-                ],
-                "log_for_invalid_gff3": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f"
-                    ]
-                ],
-                "valid_gff3": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34",
-                    "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd",
-                    "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae"
-                ]
-            }
-        ],
-        "timestamp": "2023-12-07T10:35:32.53584"
-    }
-}
\ No newline at end of file
diff --git a/subworkflows/pfr/gff3_validate/tests/tags.yml b/subworkflows/pfr/gff3_validate/tests/tags.yml
deleted file mode 100644
index 60ffbf0c..00000000
--- a/subworkflows/pfr/gff3_validate/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-subworkflows/gff3_validate:
-  - subworkflows/pfr/gff3_validate/**
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index f90e4354..eb68d088 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -11,8 +11,7 @@ include { GUNZIP as GUNZIP_GFF3             } from '../modules/nf-core/gunzip/ma
 include { FASTAVALIDATOR                    } from '../modules/nf-core/fastavalidator/main'
 include { FASTA_EXPLORE_SEARCH_PLOT_TIDK    } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main'
 
-include { GT_STAT                           } from '../modules/pfr/gt/stat/main'
-include { GFF3_VALIDATE                     } from '../subworkflows/pfr/gff3_validate/main'
+include { GFF3_GT_GFF3_GFF3VALIDATOR_STAT   } from '../subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main'
 include { NCBI_FCS_ADAPTOR                  } from '../modules/local/ncbi_fcs_adaptor'
 include { NCBI_FCS_GX                       } from '../subworkflows/local/ncbi_fcs_gx'
 include { ASSEMBLATHON_STATS                } from '../modules/local/assemblathon_stats'
@@ -131,30 +130,23 @@ workflow ASSEMBLYQC {
 
     ch_versions                             = ch_versions.mix(FASTAVALIDATOR.out.versions.first())
 
-    // SUBWORKFLOW: GFF3_VALIDATE
-    GFF3_VALIDATE (
+    // SUBWORKFLOW: GFF3_GT_GFF3_GFF3VALIDATOR_STAT
+    GFF3_GT_GFF3_GFF3VALIDATOR_STAT (
         ch_assembly_gff3,
         ch_valid_target_assembly
     )
 
-    ch_valid_gff3                           = GFF3_VALIDATE.out.valid_gff3
-
-    ch_invalid_gff3_log                     = GFF3_VALIDATE.out.log_for_invalid_gff3
+    ch_invalid_gff3_log                     = GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.log_for_invalid_gff3
                                             | map { meta, error_log ->
                                                 log.warn("GFF3 validation failed for ${meta.id}\n${error_log.text}")
 
                                                 [ meta, error_log ]
                                             }
 
-    ch_versions                             = ch_versions.mix(GFF3_VALIDATE.out.versions)
-
-    // MODULE: GT_STAT
-    GT_STAT ( ch_valid_gff3 )
-
-    ch_gt_stats                             = GT_STAT.out.stats
+    ch_gt_stats                             = GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.gff3_stats
                                             | map { meta, yml -> yml }
 
-    ch_versions                             = ch_versions.mix(GT_STAT.out.versions.first())
+    ch_versions                             = ch_versions.mix(GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.versions)
 
     // MODULE: NCBI_FCS_ADAPTOR
     ch_fcs_adaptor_inputs                   = params.ncbi_fcs_adaptor_skip

From 77e752a60fb3e9a7cf9082c9e922981c0261cb52 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 17 May 2024 11:46:00 +1200
Subject: [PATCH 40/76] Replaced local BUSCO module with FASTA_GXF_BUSCO_PLOT

---
 CHANGELOG.md                                  |   1 +
 CITATIONS.md                                  |   4 +
 README.md                                     |  38 +-
 bin/assemblyqc.py                             |   4 +
 bin/report_modules/parsers/busco_parser.py    | 135 ++--
 bin/report_modules/templates/base.html        |   8 +
 bin/report_modules/templates/busco/busco.html |   2 +-
 .../templates/busco_gff/busco_gff.html        |  25 +
 .../templates/busco_gff/dropdown.html         |  13 +
 .../templates/busco_gff/report_contents.html  |  77 +++
 .../templates/busco_gff/summary_contents.html |  37 ++
 .../gff3_validate/gff3_validate.html          |  35 +-
 conf/modules.config                           |  34 +-
 conf/test_full.config                         |   2 +-
 docs/contributors.sh                          |   2 +-
 docs/parameters.md                            |  16 +-
 modules.json                                  |  20 +
 modules/local/busco.nf                        |  56 --
 modules/local/createreport.nf                 |  33 +-
 modules/pfr/busco/busco/environment.yml       |   7 +
 modules/pfr/busco/busco/main.nf               | 107 +++
 modules/pfr/busco/busco/meta.yml              |  98 +++
 modules/pfr/busco/busco/tests/main.nf.test    | 419 ++++++++++++
 .../pfr/busco/busco/tests/main.nf.test.snap   | 230 +++++++
 .../busco/tests/nextflow.augustus.config      |   5 +
 modules/pfr/busco/busco/tests/nextflow.config |   5 +
 .../busco/busco/tests/nextflow.metaeuk.config |   5 +
 modules/pfr/busco/busco/tests/old_test.yml    | 624 ++++++++++++++++++
 modules/pfr/busco/busco/tests/tags.yml        |   2 +
 .../pfr/busco/generateplot/environment.yml    |   9 +
 .../busco/generateplot/main.nf}               |  25 +-
 modules/pfr/busco/generateplot/meta.yml       |  35 +
 .../pfr/busco/generateplot/tests/main.nf.test |  72 ++
 .../generateplot/tests/main.nf.test.snap      |  37 ++
 modules/pfr/busco/generateplot/tests/tags.yml |   2 +
 modules/pfr/gffread/environment.yml           |   7 +
 modules/pfr/gffread/main.nf                   |  60 ++
 modules/pfr/gffread/meta.yml                  |  55 ++
 modules/pfr/gffread/tests/main.nf.test        | 223 +++++++
 modules/pfr/gffread/tests/main.nf.test.snap   | 272 ++++++++
 .../pfr/gffread/tests/nextflow-fasta.config   |   5 +
 .../pfr/gffread/tests/nextflow-gff3.config    |   5 +
 modules/pfr/gffread/tests/nextflow.config     |   5 +
 modules/pfr/gffread/tests/tags.yml            |   2 +
 nextflow_schema.json                          |   9 +-
 subworkflows/local/fasta_busco_plot.nf        |  37 --
 subworkflows/pfr/fasta_gxf_busco_plot/main.nf | 168 +++++
 .../pfr/fasta_gxf_busco_plot/meta.yml         | 106 +++
 .../fasta_gxf_busco_plot/tests/main.nf.test   | 104 +++
 .../tests/main.nf.test.snap                   | 231 +++++++
 .../tests/nextflow.config                     |   5 +
 .../pfr/fasta_gxf_busco_plot/tests/tags.yml   |   2 +
 tests/stub/stub.config                        |   2 +-
 workflows/assemblyqc.nf                       |  75 ++-
 54 files changed, 3309 insertions(+), 288 deletions(-)
 create mode 100644 bin/report_modules/templates/busco_gff/busco_gff.html
 create mode 100644 bin/report_modules/templates/busco_gff/dropdown.html
 create mode 100644 bin/report_modules/templates/busco_gff/report_contents.html
 create mode 100644 bin/report_modules/templates/busco_gff/summary_contents.html
 delete mode 100644 modules/local/busco.nf
 create mode 100644 modules/pfr/busco/busco/environment.yml
 create mode 100644 modules/pfr/busco/busco/main.nf
 create mode 100644 modules/pfr/busco/busco/meta.yml
 create mode 100644 modules/pfr/busco/busco/tests/main.nf.test
 create mode 100644 modules/pfr/busco/busco/tests/main.nf.test.snap
 create mode 100644 modules/pfr/busco/busco/tests/nextflow.augustus.config
 create mode 100644 modules/pfr/busco/busco/tests/nextflow.config
 create mode 100644 modules/pfr/busco/busco/tests/nextflow.metaeuk.config
 create mode 100644 modules/pfr/busco/busco/tests/old_test.yml
 create mode 100644 modules/pfr/busco/busco/tests/tags.yml
 create mode 100644 modules/pfr/busco/generateplot/environment.yml
 rename modules/{local/busco_plot.nf => pfr/busco/generateplot/main.nf} (54%)
 create mode 100644 modules/pfr/busco/generateplot/meta.yml
 create mode 100644 modules/pfr/busco/generateplot/tests/main.nf.test
 create mode 100644 modules/pfr/busco/generateplot/tests/main.nf.test.snap
 create mode 100644 modules/pfr/busco/generateplot/tests/tags.yml
 create mode 100644 modules/pfr/gffread/environment.yml
 create mode 100644 modules/pfr/gffread/main.nf
 create mode 100644 modules/pfr/gffread/meta.yml
 create mode 100644 modules/pfr/gffread/tests/main.nf.test
 create mode 100644 modules/pfr/gffread/tests/main.nf.test.snap
 create mode 100644 modules/pfr/gffread/tests/nextflow-fasta.config
 create mode 100644 modules/pfr/gffread/tests/nextflow-gff3.config
 create mode 100644 modules/pfr/gffread/tests/nextflow.config
 create mode 100644 modules/pfr/gffread/tests/tags.yml
 delete mode 100644 subworkflows/local/fasta_busco_plot.nf
 create mode 100644 subworkflows/pfr/fasta_gxf_busco_plot/main.nf
 create mode 100644 subworkflows/pfr/fasta_gxf_busco_plot/meta.yml
 create mode 100644 subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test
 create mode 100644 subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test.snap
 create mode 100644 subworkflows/pfr/fasta_gxf_busco_plot/tests/nextflow.config
 create mode 100644 subworkflows/pfr/fasta_gxf_busco_plot/tests/tags.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 45250fba..29047314 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,6 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 12. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
 13. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
 14. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
+15. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
 
 ### `Fixed`
 
diff --git a/CITATIONS.md b/CITATIONS.md
index dcd44eed..4de821db 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -40,6 +40,10 @@
 
   > Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. 2021. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes, Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654, <https://doi.org/10.1093/molbev/msab199>
 
+- GFFREAD, [MIT](https://github.com/gpertea/gffread/blob/master/LICENSE)
+
+  > Pertea G, Pertea M. GFF Utilities: GffRead and GffCompare. F1000Res. 2020 Apr 28;9:ISCB Comm J-304. doi: <http://doi.org/10.12688/f1000research.23297.2>. PMID: 32489650; PMCID: PMC7222033.
+
 - TIDK, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE)
 
   > <https://github.com/tolkit/telomeric-identifier>
diff --git a/README.md b/README.md
index 20540811..febc6ddc 100644
--- a/README.md
+++ b/README.md
@@ -62,7 +62,7 @@ flowchart LR
 - [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification
 - [HIC CONTACT MAP](https://github.com/igvteam/juicebox.js): Alignment and visualisation of HiC data
 - SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer), [CIRCOS](http://circos.ca/documentation/) and [PLOTLY](https://plotly.com)
-- [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK): k-mer based assembly evaluation
+- [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK): K-mer completeness, consensus quality and phasing assessment
 
 ## Usage
 
@@ -114,24 +114,30 @@ We thank the following people for their extensive assistance in the development
 
 The pipeline uses nf-core modules contributed by following authors.
 
-<a href="https://github.com/adamrtalbot"><img src="https://github.com/adamrtalbot.png" width="50" height="50"></a>
-<a href="https://github.com/drpatelh"><img src="https://github.com/drpatelh.png" width="50" height="50"></a>
-<a href="https://github.com/erikrikarddaniel"><img src="https://github.com/erikrikarddaniel.png" width="50" height="50"></a>
-<a href="https://github.com/ewels"><img src="https://github.com/ewels.png" width="50" height="50"></a>
-<a href="https://github.com/felixkrueger"><img src="https://github.com/felixkrueger.png" width="50" height="50"></a>
-<a href="https://github.com/friederikehanssen"><img src="https://github.com/friederikehanssen.png" width="50" height="50"></a>
 <a href="https://github.com/gallvp"><img src="https://github.com/gallvp.png" width="50" height="50"></a>
-<a href="https://github.com/grst"><img src="https://github.com/grst.png" width="50" height="50"></a>
-<a href="https://github.com/jeremy1805"><img src="https://github.com/jeremy1805.png" width="50" height="50"></a>
-<a href="https://github.com/jfy133"><img src="https://github.com/jfy133.png" width="50" height="50"></a>
-<a href="https://github.com/joon-klaps"><img src="https://github.com/joon-klaps.png" width="50" height="50"></a>
-<a href="https://github.com/joseespinosa"><img src="https://github.com/joseespinosa.png" width="50" height="50"></a>
-<a href="https://github.com/kevinmenden"><img src="https://github.com/kevinmenden.png" width="50" height="50"></a>
-<a href="https://github.com/lescai"><img src="https://github.com/lescai.png" width="50" height="50"></a>
+<a href="https://github.com/drpatelh"><img src="https://github.com/drpatelh.png" width="50" height="50"></a>
+<a href="https://github.com/midnighter"><img src="https://github.com/midnighter.png" width="50" height="50"></a>
 <a href="https://github.com/mahesh-panchal"><img src="https://github.com/mahesh-panchal.png" width="50" height="50"></a>
-<a href="https://github.com/matthdsm"><img src="https://github.com/matthdsm.png" width="50" height="50"></a>
+<a href="https://github.com/adamrtalbot"><img src="https://github.com/adamrtalbot.png" width="50" height="50"></a>
 <a href="https://github.com/maxulysse"><img src="https://github.com/maxulysse.png" width="50" height="50"></a>
-<a href="https://github.com/midnighter"><img src="https://github.com/midnighter.png" width="50" height="50"></a>
+<a href="https://github.com/matthdsm"><img src="https://github.com/matthdsm.png" width="50" height="50"></a>
+<a href="https://github.com/joseespinosa"><img src="https://github.com/joseespinosa.png" width="50" height="50"></a>
+<a href="https://github.com/jfy133"><img src="https://github.com/jfy133.png" width="50" height="50"></a>
+<a href="https://github.com/ewels"><img src="https://github.com/ewels.png" width="50" height="50"></a>
+<a href="https://github.com/priyanka-surana"><img src="https://github.com/priyanka-surana.png" width="50" height="50"></a>
+<a href="https://github.com/phue"><img src="https://github.com/phue.png" width="50" height="50"></a>
+<a href="https://github.com/muffato"><img src="https://github.com/muffato.png" width="50" height="50"></a>
+<a href="https://github.com/lescai"><img src="https://github.com/lescai.png" width="50" height="50"></a>
+<a href="https://github.com/kevinmenden"><img src="https://github.com/kevinmenden.png" width="50" height="50"></a>
+<a href="https://github.com/jvhagey"><img src="https://github.com/jvhagey.png" width="50" height="50"></a>
+<a href="https://github.com/joon-klaps"><img src="https://github.com/joon-klaps.png" width="50" height="50"></a>
+<a href="https://github.com/jeremy1805"><img src="https://github.com/jeremy1805.png" width="50" height="50"></a>
+<a href="https://github.com/grst"><img src="https://github.com/grst.png" width="50" height="50"></a>
+<a href="https://github.com/friederikehanssen"><img src="https://github.com/friederikehanssen.png" width="50" height="50"></a>
+<a href="https://github.com/felixkrueger"><img src="https://github.com/felixkrueger.png" width="50" height="50"></a>
+<a href="https://github.com/erikrikarddaniel"><img src="https://github.com/erikrikarddaniel.png" width="50" height="50"></a>
+<a href="https://github.com/edmundmiller"><img src="https://github.com/edmundmiller.png" width="50" height="50"></a>
+<a href="https://github.com/charles-plessy"><img src="https://github.com/charles-plessy.png" width="50" height="50"></a>
 
 ## Contributions and Support
 
diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py
index 73f7780c..f5d9d6bc 100755
--- a/bin/assemblyqc.py
+++ b/bin/assemblyqc.py
@@ -43,6 +43,10 @@
     data_from_tools = {**data_from_tools, **parse_assemblathon_stats_folder()}
     data_from_tools = {**data_from_tools, **parse_genometools_gt_stat_folder()}
     data_from_tools = {**data_from_tools, **parse_busco_folder()}
+    data_from_tools = {
+        **data_from_tools,
+        **parse_busco_folder("busco_gff_outputs", "BUSCO_GFF"),
+    }
     data_from_tools = {**data_from_tools, **parse_tidk_folder()}
     data_from_tools = {**data_from_tools, **parse_lai_folder()}
     data_from_tools = {**data_from_tools, **parse_kraken2_folder()}
diff --git a/bin/report_modules/parsers/busco_parser.py b/bin/report_modules/parsers/busco_parser.py
index 74c6fb05..44d52d0f 100644
--- a/bin/report_modules/parsers/busco_parser.py
+++ b/bin/report_modules/parsers/busco_parser.py
@@ -11,70 +11,64 @@
 
 class BuscoParser:
     def __init__(self, file_data):
-        self.file_data = file_data
-        self.stats_dict = {}
+        self.file_text = file_data
 
     def parse_report(self):
-        self.stats_dict["version"] = self.get_busco_version(self.file_data)
-        self.stats_dict["lineage"] = self.get_lineage_dataset(self.file_data)
-        self.stats_dict["created"] = self.get_creation_date(self.file_data)
-        self.stats_dict["mode"] = self.get_run_mode(self.file_data)
-        self.stats_dict["predictor"] = self.get_gene_predictor(self.file_data)
-        self.stats_dict["search_percentages"] = self.get_busco_percentages(
-            self.file_data
+        stats_dict = {}
+        stats_dict["version"] = self.get_busco_version()
+        stats_dict["lineage"] = self.get_lineage_dataset()
+        stats_dict["created"] = self.get_creation_date()
+        stats_dict["mode"] = self.get_run_mode()
+        stats_dict["predictor"] = self.get_gene_predictor()
+        stats_dict["search_percentages"] = self.get_busco_percentages()
+        (stats_dict["results_dict"], stats_dict["results_table"]) = (
+            self.get_busco_result_table()
         )
-        self.stats_dict["dependencies"] = self.get_deps_and_versions(self.file_data)
-        self.stats_dict["results_table"] = self.get_busco_result_table(self.file_data)
-
-        # include busco results dictionary for use in json dump
-        self.stats_dict["results_dict"] = self.get_busco_result_dict(self.file_data)
-        # include dependencies dictionary for use in json dump
-        self.stats_dict["dependencies_dict"] = self.get_deps_and_versions_dict(
-            self.file_data
+
+        (stats_dict["dependencies_dict"], stats_dict["dependencies"]) = (
+            self.get_deps_and_versions()
         )
 
-        return self.stats_dict
+        return stats_dict
 
-    def get_busco_version(self, data):
+    def get_busco_version(self):
         p = re.compile("BUSCO version is: (.*)")
-        result = p.search(data).group(1).strip()
+        result = p.search(self.file_text).group(1).strip()
         return result
 
-    def get_lineage_dataset(self, data):
+    def get_lineage_dataset(self):
         p = re.compile("The lineage dataset is: (.*)")
-        result = p.search(data).group(1).split()[0]
+        result = p.search(self.file_text).group(1).split()[0]
         return result
 
-    def get_creation_date(self, data):
+    def get_creation_date(self):
         p = re.compile("The lineage dataset is: (.*)")
-        result = p.search(data)
+        result = p.search(self.file_text)
         result = result.group(1).split()[3][:-1]
         return result
 
-    def get_run_mode(self, data):
+    def get_run_mode(self):
         p = re.compile("BUSCO was run in mode: (.*)")
-        result = p.search(data).group(1)
+        result = p.search(self.file_text).group(1)
         return result
 
-    def get_gene_predictor(self, data):
+    def get_gene_predictor(self):
         p = re.compile("Gene predictor used: (.*)")
-        gene_predictor = p.search(data)
+        gene_predictor = p.search(self.file_text)
 
         if gene_predictor == None:
             return "None"
 
         result = gene_predictor.group(1)
-        q = re.compile(f"{gene_predictor.group(1)}: (.*)")
-        predictor_version = q.search(data)
         return result
 
-    def get_busco_percentages(self, data):
+    def get_busco_percentages(self):
         p = re.compile("C:(.*)")
-        result = p.search(data).group(0).strip()
+        result = p.search(self.file_text).group(0).strip()
         return result
 
-    def get_deps_and_versions(self, file_data):
-        list_of_lines = file_data.split("\n")
+    def get_deps_and_versions(self):
+        list_of_lines = self.file_text.split("\n")
         for index, line in enumerate(list_of_lines):
             if "Dependencies and versions" in line:
                 all_deps = (
@@ -88,44 +82,28 @@ def get_deps_and_versions(self, file_data):
             dependency = dep.split(":")[0]
             version = dep.split(":")[1].strip()
             dep_dict[f"{dependency}"] = f"{version}"
+
         df = pd.DataFrame(dep_dict.items(), columns=["Dependency", "Version"])
 
         col_names = ["Dependency", "Version"]
         table = tabulate(
             df, headers=col_names, tablefmt="html", numalign="left", showindex=False
         )
-        return table
-
-    # get dependencies dictionary instead of table to use in json dump
-    def get_deps_and_versions_dict(self, file_data):
-        list_of_lines = file_data.split("\n")
-        for index, line in enumerate(list_of_lines):
-            if "Dependencies and versions" in line:
-                all_deps = (
-                    "".join(list_of_lines[max(0, index + 1) : len(list_of_lines) - 2])
-                    .replace("\t", "\n")
-                    .strip()
-                )
-
-        dep_dict = {}
-        for dep in all_deps.splitlines():
-            dependency = dep.split(":")[0]
-            version = dep.split(":")[1].strip()
-            dep_dict[f"{dependency}"] = f"{version}"
 
-        return dep_dict
+        return (dep_dict, table)
 
-    def get_busco_result_table(self, file_data):
-        list_of_lines = file_data.split("\n")
+    def get_busco_result_table(self):
+        list_of_lines = self.file_text.split("\n")
+        end_index = len(list_of_lines) - 1
         for index, line in enumerate(list_of_lines):
-            if "Assembly Statistics" in line:
-                stats_index = index
+            if ("Assembly Statistics" in line) or ("Dependencies and versions" in line):
+                end_index = index
                 break
 
         results_dict = {}
         for index, line in enumerate(list_of_lines):
             if "C:" in line:
-                for i in range(index + 1, stats_index - 1):
+                for i in range(index + 1, end_index - 1):
                     number = list_of_lines[i].split("\t")[1]
                     descr = list_of_lines[i].split("\t")[2]
 
@@ -135,28 +113,10 @@ def get_busco_result_table(self, file_data):
         table = tabulate(
             df, headers=col_names, tablefmt="html", numalign="left", showindex=False
         )
-        return table
+        return (results_dict, table)
 
-    # get results dictionary instead of table to use in json dump
-    def get_busco_result_dict(self, file_data):
-        list_of_lines = file_data.split("\n")
-        for index, line in enumerate(list_of_lines):
-            if "Assembly Statistics" in line:
-                stats_index = index
-
-        results_dict = {}
-        for index, line in enumerate(list_of_lines):
-            if "C:" in line:
-                for i in range(index + 1, stats_index - 1):
-                    number = list_of_lines[i].split("\t")[1]
-                    descr = list_of_lines[i].split("\t")[2]
 
-                    results_dict[f"{descr}"] = f"{number}"
-
-        return results_dict
-
-
-def parse_busco_folder(folder_name="busco_outputs"):
+def parse_busco_folder(folder_name="busco_outputs", data_key="BUSCO"):
     dir = os.getcwdb().decode()
     busco_folder_path = Path(f"{dir}/{folder_name}")
 
@@ -172,27 +132,28 @@ def parse_busco_folder(folder_name="busco_outputs"):
     ext = str(plot_path).split(".")[-1]
     busco_plot_url = f"data:image/{ext};base64,{base64_utf8_str}"
 
-    data = {"BUSCO": []}
+    data = {data_key: []}
 
     for file in list_of_files:
-        file_data = ""
         with open(file, "r") as file:
-            lines = file.readlines()
-            for line in lines:
-                file_data += line
-        parser = BuscoParser(file_data)
+            file_text = file.read()
+
+        parser = BuscoParser(file_text)
+
         file_tokens = re.findall(
             r"short_summary.specific.([\w]+).([\w]+)_([a-zA-Z0-9]+).txt",
             os.path.basename(str(file)),
         )[0]
+
         stats = {
             "hap": file_tokens[1],
             "lineage": file_tokens[0],
             **parser.parse_report(),
         }
-        data["BUSCO"].append(stats)
 
-    data["BUSCO"] = sort_list_of_results(data["BUSCO"], "hap")
-    data["BUSCO"][0]["busco_plot"] = busco_plot_url
+        data[data_key].append(stats)
+
+    data[data_key] = sort_list_of_results(data[data_key], "hap")
+    data[data_key][0]["busco_plot"] = busco_plot_url
 
     return data
diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html
index 31d52c4c..d2478dfb 100644
--- a/bin/report_modules/templates/base.html
+++ b/bin/report_modules/templates/base.html
@@ -40,6 +40,10 @@
     <button class="tablinks active" onclick="openTool(event, 'BUSCO')">BUSCO</button>
     {% endif %}
 
+    {% if 'BUSCO_GFF' in all_stats_dicts %}
+    <button class="tablinks active" onclick="openTool(event, 'BUSCO_GFF')">BUSCO GFF</button>
+    {% endif %}
+
     {% if 'TIDK' in all_stats_dicts %}
     <button class="tablinks" onclick="openTool(event, 'TIDK')">TIDK</button>
     {% endif %}
@@ -100,6 +104,10 @@
   {% include 'busco/busco.html' %}
   {% endif %}
 
+  {% if 'BUSCO_GFF' in all_stats_dicts %}
+  {% include 'busco_gff/busco_gff.html' %}
+  {% endif %}
+
   {% if 'TIDK' in all_stats_dicts %}
   {% include 'tidk/tidk.html' %}
   {% endif %}
diff --git a/bin/report_modules/templates/busco/busco.html b/bin/report_modules/templates/busco/busco.html
index 4f6f9b3a..20e8fc04 100644
--- a/bin/report_modules/templates/busco/busco.html
+++ b/bin/report_modules/templates/busco/busco.html
@@ -11,7 +11,7 @@
             viral genomes. arXiv:2106.11799 [q-bio] [Internet]. Available from:
             <a href="http://arxiv.org/abs/2106.11799" target="_blank">arxiv.org/abs/2106.11799</a>
         </p>
-        <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['BUSCO']['busco'] }}</b></p>
+        <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['BUSCO_ASSEMBLY']['busco'] }}</b></p>
     </div>
     {% include 'busco/dropdown.html' %} {% include 'busco/summary_contents.html' %} {% include
     'busco/report_contents.html' %}
diff --git a/bin/report_modules/templates/busco_gff/busco_gff.html b/bin/report_modules/templates/busco_gff/busco_gff.html
new file mode 100644
index 00000000..654a59c9
--- /dev/null
+++ b/bin/report_modules/templates/busco_gff/busco_gff.html
@@ -0,0 +1,25 @@
+<div id="BUSCO_GFF" class="tabcontent" style="display: none">
+  <div class="section-para-wrapper">
+    <p class="section-para">
+      BUSCO estimates the completeness and redundancy of processed genomic data based on universal single-copy
+      orthologs. GFFREAD is used to obtain protein sequences from assembly FASTA and annotation GFF3 files.
+    </p>
+    <p class="section-para"><b>Reference:</b></p>
+    <p class="section-para">
+      Manni M., Berkeley M.R., Seppey M., Simao F.A., Zdobnov E.M. 2021. BUSCO update: novel and streamlined
+      workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic, prokaryotic, and
+      viral genomes. arXiv:2106.11799 [q-bio] [Internet]. Available from:
+      <a href="http://arxiv.org/abs/2106.11799" target="_blank">arxiv.org/abs/2106.11799</a>
+    </p>
+    <p class="section-para">
+      Pertea G, Pertea M. GFF Utilities: GffRead and GffCompare. F1000Res. 2020 Apr 28;9:ISCB Comm J-304. doi: <a
+        href="http://doi.org/10.12688/f1000research.23297.2" target="_blank">10.12688/f1000research.23297.2</a>. PMID:
+      32489650; PMCID: PMC7222033.
+    </p>
+    <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['BUSCO_ANNOTATION']['busco'] }} (BUSCO), {{
+        all_stats_dicts['VERSIONS']['EXTRACT_PROTEINS']['gffread'] }} (GFFREAD)</b></p>
+  </div>
+  {% include 'busco_gff/dropdown.html' %}
+  {% include 'busco_gff/summary_contents.html' %}
+  {% include 'busco_gff/report_contents.html' %}
+</div>
diff --git a/bin/report_modules/templates/busco_gff/dropdown.html b/bin/report_modules/templates/busco_gff/dropdown.html
new file mode 100644
index 00000000..982255f5
--- /dev/null
+++ b/bin/report_modules/templates/busco_gff/dropdown.html
@@ -0,0 +1,13 @@
+<div class="dropdown">
+  <div class="dropdown_content">
+    <select id="selector_BUSCO_GFF" onchange="showContent('BUSCO_GFF')">
+      <option value="summary_BUSCO_GFF">Summary</option>
+      {% set str_busco = 'BUSCO_GFF' %} {% set str_lin = 'lineage' %} {% set str_hap = 'hap' %} {% for item in
+      range(all_stats_dicts["BUSCO_GFF"]|length) %}
+      <option value="{{all_stats_dicts['BUSCO_GFF'][item]['hap']}}_{{all_stats_dicts['BUSCO_GFF'][item]['lineage']}}_BUSCO_GFF">
+        {{ all_stats_dicts['BUSCO_GFF'][item][str_hap] }} : {{ all_stats_dicts['BUSCO_GFF'][item][str_lin] }}
+      </option>
+      {% endfor %}
+    </select>
+  </div>
+</div>
diff --git a/bin/report_modules/templates/busco_gff/report_contents.html b/bin/report_modules/templates/busco_gff/report_contents.html
new file mode 100644
index 00000000..55be556f
--- /dev/null
+++ b/bin/report_modules/templates/busco_gff/report_contents.html
@@ -0,0 +1,77 @@
+{% for item in range(all_stats_dicts["BUSCO_GFF"]|length) %}
+<div
+    id="{{ all_stats_dicts['BUSCO_GFF'][item]['hap'] }}_{{ all_stats_dicts['BUSCO_GFF'][item]['lineage'] }}_BUSCO_GFF"
+    class="tabcontent-BUSCO_GFF"
+    style="display: none"
+>
+    <div class="results-section">
+        <div class="section-heading-wrapper">
+            <div class="section-heading">Results</div>
+        </div>
+
+        <div class="table-outer">
+            <div class="table-wrapper">
+                <table>
+                    <thead>
+                        <tr>
+                            <th style="text-align: left">Event</th>
+                            <th>Value</th>
+                        </tr>
+                    </thead>
+                    <tbody>
+                        <tr>
+                            <td style="text-align: left">Search Percentages</td>
+                            <td>{{ all_stats_dicts['BUSCO_GFF'][item]['search_percentages'] }}</td>
+                        </tr>
+                    </tbody>
+                </table>
+            </div>
+        </div>
+
+        <div class="table-outer">
+            <div class="table-wrapper">{{ all_stats_dicts['BUSCO_GFF'][item]['results_table'] }}</div>
+        </div>
+    </div>
+
+    <div class="parameters-section">
+        <div class="section-heading-wrapper">
+            <div class="section-heading">Parameters and Dependencies</div>
+        </div>
+
+        <div class="table-outer">
+            <div class="table-wrapper">
+                <table>
+                    <thead>
+                        <tr>
+                            <th style="text-align: left">Parameter</th>
+                            <th>Value</th>
+                        </tr>
+                    </thead>
+                    <tbody>
+                        <tr>
+                            <td style="text-align: left">Version</td>
+                            <td>{{ all_stats_dicts['BUSCO_GFF'][item]['version'] }}</td>
+                        </tr>
+                        <tr>
+                            <td style="text-align: left">Lineage create on</td>
+                            <td>{{ all_stats_dicts['BUSCO_GFF'][item]['created'] }}</td>
+                        </tr>
+                        <tr>
+                            <td style="text-align: left">mode</td>
+                            <td>{{ all_stats_dicts['BUSCO_GFF'][item]['mode'] }}</td>
+                        </tr>
+                        <tr>
+                            <td style="text-align: left">predictor</td>
+                            <td>{{ all_stats_dicts['BUSCO_GFF'][item]['predictor'] }}</td>
+                        </tr>
+                    </tbody>
+                </table>
+            </div>
+        </div>
+
+        <div class="table-outer">
+            <div class="table-wrapper">{{ all_stats_dicts['BUSCO_GFF'][item]['dependencies'] }}</div>
+        </div>
+    </div>
+</div>
+{% endfor %}
diff --git a/bin/report_modules/templates/busco_gff/summary_contents.html b/bin/report_modules/templates/busco_gff/summary_contents.html
new file mode 100644
index 00000000..d99344d2
--- /dev/null
+++ b/bin/report_modules/templates/busco_gff/summary_contents.html
@@ -0,0 +1,37 @@
+<div id="summary_BUSCO_GFF" class="tabcontent-BUSCO_GFF" style="display: block">
+    <div class="results-section">
+        <div class="section-heading-wrapper">
+            <div class="section-heading">Summary</div>
+        </div>
+
+        <div class="table-outer">
+            <div class="table-wrapper">
+                <table>
+                    <thead>
+                        <tr>
+                            <th width="20%" style="text-align: left">Annotation</th>
+                            <th width="20%" style="text-align: left">Lineage</th>
+                            <th>Percentages</th>
+                        </tr>
+                    </thead>
+                    <tbody>
+                        {% for item in range(all_stats_dicts["BUSCO_GFF"]|length) %}
+                        <tr>
+                            <td style="text-align: left; font-size: 16px">
+                                {{ all_stats_dicts['BUSCO_GFF'][item]['hap'] }}
+                            </td>
+                            <td style="text-align: left; font-size: 16px">
+                                {{ all_stats_dicts['BUSCO_GFF'][item]['lineage'] }}
+                            </td>
+                            <td>{{ all_stats_dicts['BUSCO_GFF'][item]['search_percentages'] }}</td>
+                        </tr>
+                        {% endfor %}
+                    </tbody>
+                </table>
+            </div>
+        </div>
+    </div>
+    <div class="image-wrapper">
+        <img src="{{ all_stats_dicts['BUSCO_GFF'][0]['busco_plot'] }}" alt="" />
+    </div>
+</div>
diff --git a/bin/report_modules/templates/gff3_validate/gff3_validate.html b/bin/report_modules/templates/gff3_validate/gff3_validate.html
index 01fa2754..d43fb8db 100644
--- a/bin/report_modules/templates/gff3_validate/gff3_validate.html
+++ b/bin/report_modules/templates/gff3_validate/gff3_validate.html
@@ -1,22 +1,17 @@
 <div id="GFF3_VALIDATE" class="tabcontent" style="display: none">
-    <div class="section-para-wrapper">
-        <p class="section-para">
-            GFF3 validation is conducted with gt/gff3 and gt/gff3validate tools from GenomeTools and a
-            <a
-                href="https://github.com/Plant-Food-Research-Open/assembly_qc/blob/v1.3/bin/check_gff3_fasta_corresp_3031aca.sh"
-                target="_blank"
-                >custom script</a
-            >
-            which check correspondence between FASTA and GFF3 sequence identifiers and coordinates.
-        </p>
-        <p class="section-para"><b>Reference:</b></p>
-        <p class="section-para">
-            Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient
-            Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and
-            Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi:
-            <a href="https://doi.org/10.1109/TCBB.2013.68" target="_blank">10.1109/TCBB.2013.68</a>
-        </p>
-        <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['GT_GFF3']['genometools'] }}</b></p>
-    </div>
-    {% include 'gff3_validate/dropdown.html' %} {% include 'gff3_validate/report_contents.html' %}
+  <div class="section-para-wrapper">
+    <p class="section-para">
+      GFF3 validation is conducted with gt/gff3 and gt/gff3validate tools from GenomeTools and a custom script which
+      check correspondence between FASTA and GFF3 sequence identifiers and coordinates.
+    </p>
+    <p class="section-para"><b>Reference:</b></p>
+    <p class="section-para">
+      Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient
+      Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and
+      Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi:
+      <a href="https://doi.org/10.1109/TCBB.2013.68" target="_blank">10.1109/TCBB.2013.68</a>
+    </p>
+    <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['GT_GFF3']['genometools'] }}</b></p>
+  </div>
+  {% include 'gff3_validate/dropdown.html' %} {% include 'gff3_validate/report_contents.html' %}
 </div>
diff --git a/conf/modules.config b/conf/modules.config
index 0359b8a1..028955ff 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -66,17 +66,43 @@ process {
         ]
     }
 
-    withName: BUSCO {
+    withName: '.*:FASTA_GXF_BUSCO_PLOT:BUSCO_ASSEMBLY' {
+        ext.args = '--metaeuk'
         publishDir = [
-            path: { "${params.outdir}/busco" },
+            path: { "${params.outdir}/busco/fasta" },
+            mode: params.publish_dir_mode,
+            pattern: 'short_summary.*.txt',
+            saveAs: { filename -> "short_summary.specific.${meta.id}.${lineage}.txt" }
+        ]
+    }
+
+    withName: '.*:FASTA_GXF_BUSCO_PLOT:PLOT_ASSEMBLY' {
+        ext.prefix = 'busco_figure'
+        publishDir = [
+            path: { "${params.outdir}/busco/fasta" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
         ]
     }
 
-    withName: BUSCO_PLOT {
+    withName: '.*:FASTA_GXF_BUSCO_PLOT:EXTRACT_PROTEINS' {
+        ext.args = '-y'
+    }
+
+    withName: '.*:FASTA_GXF_BUSCO_PLOT:BUSCO_ANNOTATION' {
+        ext.args = '--metaeuk'
+        publishDir = [
+            path: { "${params.outdir}/busco/gff" },
+            mode: params.publish_dir_mode,
+            pattern: 'short_summary.*.txt',
+            saveAs: { filename -> "short_summary.specific.${meta.id}.${lineage}.txt" }
+        ]
+    }
+
+    withName: '.*:FASTA_GXF_BUSCO_PLOT:PLOT_ANNOTATION' {
+        ext.prefix = 'busco_figure'
         publishDir = [
-            path: { "${params.outdir}" },
+            path: { "${params.outdir}/busco/gff" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
         ]
diff --git a/conf/test_full.config b/conf/test_full.config
index 9c7b5ad8..e3c9b3c9 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -24,7 +24,7 @@ params {
     // ncbi_fcs_gx_db_path      = 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2023-01-24'
 
     busco_skip                  = false
-    busco_mode                  = 'geno'
+    busco_mode                  = 'genome'
     busco_lineage_datasets      = 'fungi_odb10 hypocreales_odb10'
 
     tidk_skip                   = false
diff --git a/docs/contributors.sh b/docs/contributors.sh
index f3839348..e694e491 100755
--- a/docs/contributors.sh
+++ b/docs/contributors.sh
@@ -2,4 +2,4 @@
 
 module_authors=$(find ./modules -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '"' | tr '[:upper:]' '[:lower:]' | awk '{print $2}')
 workflow_authors=$(find ./subworkflows -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '"' | tr '[:upper:]' '[:lower:]' | awk '{print $2}')
-echo -e "${module_authors}\n${workflow_authors}" | sort -V | uniq | sed -n 's|@\(.*\)|<a href="https://github.com/\1"><img src="https://github.com/\1.png" width="50" height="50"></a>|p'
+echo -e "${module_authors}\n${workflow_authors}" | sort -V | uniq -c | sort -k1,1 -r | awk '{print $2}' | sed -n 's|@\(.*\)|<a href="https://github.com/\1"><img src="https://github.com/\1.png" width="50" height="50"></a>|p'
diff --git a/docs/parameters.md b/docs/parameters.md
index b1fd54d3..9c8506c2 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -32,7 +32,7 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 | Parameter                | Description                                                                                                    | Type      | Default | Required | Hidden |
 | ------------------------ | -------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
 | `busco_skip`             | Skip BUSCO                                                                                                     | `boolean` | True    |          |        |
-| `busco_mode`             | BUSCO mode: 'geno' for genome, 'tran' for transcriptome, 'prot' for proteins                                   | `string`  |         |          |        |
+| `busco_mode`             | BUSCO mode: 'genome', 'transcriptome', 'proteins'                                                              | `string`  |         |          |        |
 | `busco_lineage_datasets` | BUSCO lineages. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' | `string`  |         |          |        |
 | `busco_download_path`    | Download path for BUSCO                                                                                        | `string`  |         |          |        |
 
@@ -60,12 +60,12 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## HiC options
 
-| Parameter            | Description                                                                         | Type      | Default                                           | Required | Hidden |
-| -------------------- | ----------------------------------------------------------------------------------- | --------- | ------------------------------------------------- | -------- | ------ | --- |
-| `hic`                | HiC reads path provided as a SRA ID or as paired reads with pattern '\*{1,2}.(fastq | fq).gz'   | `string`                                          |          |        |     |
-| `hic_skip_fastp`     | Skip HiC read trimming                                                              | `boolean` |                                                   |          |        |
-| `hic_skip_fastqc`    | Skip HiC read QC                                                                    | `boolean` |                                                   |          |        |
-| `hic_fastp_ext_args` | Additional parameters for fastp trimming                                            | `string`  | --qualified_quality_phred 20 --length_required 50 |          |        |
+| Parameter            | Description                                                                            | Type      | Default                                           | Required | Hidden |
+| -------------------- | -------------------------------------------------------------------------------------- | --------- | ------------------------------------------------- | -------- | ------ |
+| `hic`                | HiC reads path provided as a SRA ID or as paired reads with pattern '\*{1,2}.fastq.gz' | `string`  |                                                   |          |        |
+| `hic_skip_fastp`     | Skip HiC read trimming                                                                 | `boolean` |                                                   |          |        |
+| `hic_skip_fastqc`    | Skip HiC read QC                                                                       | `boolean` |                                                   |          |        |
+| `hic_fastp_ext_args` | Additional parameters for fastp trimming                                               | `string`  | --qualified_quality_phred 20 --length_required 50 |          |        |
 
 ## Synteny options
 
@@ -85,7 +85,7 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 | Parameter             | Description                      | Type      | Default | Required | Hidden |
 | --------------------- | -------------------------------- | --------- | ------- | -------- | ------ |
-| `merqury_skip`        |                                  | `boolean` | True    |          |        |
+| `merqury_skip`        | Skip merqury analysis            | `boolean` | True    |          |        |
 | `merqury_kmer_length` | kmer length for merqury analysis | `integer` | 21      |          |        |
 
 ## Max job request options
diff --git a/modules.json b/modules.json
index f8bc841f..0fff3420 100644
--- a/modules.json
+++ b/modules.json
@@ -5,6 +5,16 @@
         "https://github.com/PlantandFoodResearch/nxf-modules.git": {
             "modules": {
                 "pfr": {
+                    "busco/busco": {
+                        "branch": "main",
+                        "git_sha": "c3ef9317fe867609f5927588fbf17e3c3ebf389c",
+                        "installed_by": ["fasta_gxf_busco_plot"]
+                    },
+                    "busco/generateplot": {
+                        "branch": "main",
+                        "git_sha": "c3ef9317fe867609f5927588fbf17e3c3ebf389c",
+                        "installed_by": ["fasta_gxf_busco_plot"]
+                    },
                     "bwa/index": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
@@ -30,6 +40,11 @@
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
+                    "gffread": {
+                        "branch": "main",
+                        "git_sha": "c3ef9317fe867609f5927588fbf17e3c3ebf389c",
+                        "installed_by": ["fasta_gxf_busco_plot"]
+                    },
                     "gt/gff3": {
                         "branch": "main",
                         "git_sha": "84f31d4be03e2ae6bee8e0b9fdea466be8e997bb",
@@ -79,6 +94,11 @@
             },
             "subworkflows": {
                 "pfr": {
+                    "fasta_gxf_busco_plot": {
+                        "branch": "main",
+                        "git_sha": "0e4afaff4964485d2d93f1115c5254a99765b9c1",
+                        "installed_by": ["subworkflows"]
+                    },
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
                         "git_sha": "8088445a270d324cb06e085f18790839c9684754",
diff --git a/modules/local/busco.nf b/modules/local/busco.nf
deleted file mode 100644
index 1c8b3f46..00000000
--- a/modules/local/busco.nf
+++ /dev/null
@@ -1,56 +0,0 @@
-process BUSCO {
-    tag "${asm_tag}:${lineage_dataset}"
-    label 'process_high'
-
-    conda "bioconda::busco=5.6.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/busco:5.6.1--pyhdfd78af_0':
-        'biocontainers/busco:5.6.1--pyhdfd78af_0' }"
-
-    input:
-    tuple val(asm_tag), path(fasta_file)
-    val lineage_dataset
-    val mode
-    val download_path
-
-    output:
-    path "${asm_tag}/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt"  , emit: summary
-    path "versions.yml"                                                                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def lineages_path   = download_path ? "--download_path ${download_path}" : ''
-    lineage_initials    = "${lineage_dataset}".split("_")[0]
-
-    """
-    busco \\
-        -m ${mode} \\
-        -o ${asm_tag} \\
-        -i $fasta_file \\
-        -l ${lineage_dataset} \\
-        $lineages_path \\
-        -c ${task.cpus}
-
-    mv $asm_tag/short_summary.specific.${lineage_dataset}.${asm_tag}.txt \\
-        $asm_tag/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' )
-    END_VERSIONS
-    """
-
-    stub:
-    lineage_initials    = "${lineage_dataset}".split("_")[0]
-    """
-    mkdir -p $asm_tag
-    touch $asm_tag/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf
index 087bec70..b072b184 100644
--- a/modules/local/createreport.nf
+++ b/modules/local/createreport.nf
@@ -5,27 +5,28 @@ process CREATEREPORT {
     container "docker.io/gallvp/python3npkgs:v0.7"
 
     input:
-    path fastavalidator_logs, stageAs: 'fastavalidator_logs/*'
-    path gff3_validate_logs, stageAs: 'gff3_validate_logs/*'
-    path ncbi_fcs_adaptor_reports, stageAs: 'ncbi_fcs_adaptor_reports/*'
-    path fcs_gx_reports, stageAs: 'fcs_gx_reports/*'
-    path assemblathon_stats, stageAs: 'assemblathon_stats/*'
-    path genometools_gt_stats, stageAs: 'genometools_gt_stat/*'
-    path busco_outputs, stageAs: 'busco_outputs/*'
-    path tidk_plots, stageAs: 'tidk_outputs/*'
-    path lai_outputs, stageAs: 'lai_outputs/*'
-    path kraken2_outputs, stageAs: 'kraken2_outputs/*'
-    path hic_outputs, stageAs: 'hic_outputs/*'
-    path synteny_outputs, stageAs: 'synteny_outputs/*'
-    path merqury_outputs, stageAs: 'merquryfk_outputs/*'
+    path fastavalidator_logs        , stageAs: 'fastavalidator_logs/*'
+    path gff3_validate_logs         , stageAs: 'gff3_validate_logs/*'
+    path ncbi_fcs_adaptor_reports   , stageAs: 'ncbi_fcs_adaptor_reports/*'
+    path fcs_gx_reports             , stageAs: 'fcs_gx_reports/*'
+    path assemblathon_stats         , stageAs: 'assemblathon_stats/*'
+    path genometools_gt_stats       , stageAs: 'genometools_gt_stat/*'
+    path busco_outputs              , stageAs: 'busco_outputs/*'
+    path busco_gff_outputs          , stageAs: 'busco_gff_outputs/*'
+    path tidk_plots                 , stageAs: 'tidk_outputs/*'
+    path lai_outputs                , stageAs: 'lai_outputs/*'
+    path kraken2_outputs            , stageAs: 'kraken2_outputs/*'
+    path hic_outputs                , stageAs: 'hic_outputs/*'
+    path synteny_outputs            , stageAs: 'synteny_outputs/*'
+    path merqury_outputs            , stageAs: 'merquryfk_outputs/*'
     path versions
     val params_json
     val params_summary_json
 
     output:
-    path 'report.html'  , emit: html
-    path 'report.json'  , emit: json
-    path 'versions.yml' , emit: versions
+    path 'report.html'              , emit: html
+    path 'report.json'              , emit: json
+    path 'versions.yml'             , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/pfr/busco/busco/environment.yml b/modules/pfr/busco/busco/environment.yml
new file mode 100644
index 00000000..06a5d930
--- /dev/null
+++ b/modules/pfr/busco/busco/environment.yml
@@ -0,0 +1,7 @@
+name: busco_busco
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::busco=5.7.1
diff --git a/modules/pfr/busco/busco/main.nf b/modules/pfr/busco/busco/main.nf
new file mode 100644
index 00000000..f7c1a662
--- /dev/null
+++ b/modules/pfr/busco/busco/main.nf
@@ -0,0 +1,107 @@
+process BUSCO_BUSCO {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/busco:5.7.1--pyhdfd78af_0':
+        'biocontainers/busco:5.7.1--pyhdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(fasta, stageAs:'tmp_input/*')
+    val mode                              // Required:    One of genome, proteins, or transcriptome
+    val lineage                           // Required:    lineage to check against, "auto" enables --auto-lineage instead
+    path busco_lineages_path              // Recommended: path to busco lineages - downloads if not set
+    path config_file                      // Optional:    busco configuration file
+
+    output:
+    tuple val(meta), path("*-busco.batch_summary.txt")                , emit: batch_summary
+    tuple val(meta), path("short_summary.*.txt")                      , emit: short_summaries_txt   , optional: true
+    tuple val(meta), path("short_summary.*.json")                     , emit: short_summaries_json  , optional: true
+    tuple val(meta), path("*-busco/*/run_*/full_table.tsv")           , emit: full_table            , optional: true
+    tuple val(meta), path("*-busco/*/run_*/missing_busco_list.tsv")   , emit: missing_busco_list    , optional: true
+    tuple val(meta), path("*-busco/*/run_*/single_copy_proteins.faa") , emit: single_copy_proteins  , optional: true
+    tuple val(meta), path("*-busco/*/run_*/busco_sequences")          , emit: seq_dir
+    tuple val(meta), path("*-busco/*/translated_proteins")            , emit: translated_dir        , optional: true
+    tuple val(meta), path("*-busco")                                  , emit: busco_dir
+    path "versions.yml"                                               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    if ( mode !in [ 'genome', 'proteins', 'transcriptome' ] ) {
+        error "Mode must be one of 'genome', 'proteins', or 'transcriptome'."
+    }
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}-${lineage}"
+    def busco_config = config_file ? "--config $config_file" : ''
+    def busco_lineage = lineage.equals('auto') ? '--auto-lineage' : "--lineage_dataset ${lineage}"
+    def busco_lineage_dir = busco_lineages_path ? "--download_path ${busco_lineages_path}" : ''
+    """
+    # Nextflow changes the container --entrypoint to /bin/bash (container default entrypoint: /usr/local/env-execute)
+    # Check for container variable initialisation script and source it.
+    if [ -f "/usr/local/env-activate.sh" ]; then
+        set +u  # Otherwise, errors out because of various unbound variables
+        . "/usr/local/env-activate.sh"
+        set -u
+    fi
+
+    # If the augustus config directory is not writable, then copy to writeable area
+    if [ ! -w "\${AUGUSTUS_CONFIG_PATH}" ]; then
+        # Create writable tmp directory for augustus
+        AUG_CONF_DIR=\$( mktemp -d -p \$PWD )
+        cp -r \$AUGUSTUS_CONFIG_PATH/* \$AUG_CONF_DIR
+        export AUGUSTUS_CONFIG_PATH=\$AUG_CONF_DIR
+        echo "New AUGUSTUS_CONFIG_PATH=\${AUGUSTUS_CONFIG_PATH}"
+    fi
+
+    # Ensure the input is uncompressed
+    INPUT_SEQS=input_seqs
+    mkdir "\$INPUT_SEQS"
+    cd "\$INPUT_SEQS"
+    for FASTA in ../tmp_input/*; do
+        if [ "\${FASTA##*.}" == 'gz' ]; then
+            gzip -cdf "\$FASTA" > \$( basename "\$FASTA" .gz )
+        else
+            ln -s "\$FASTA" .
+        fi
+    done
+    cd ..
+
+    busco \\
+        --cpu $task.cpus \\
+        --in "\$INPUT_SEQS" \\
+        --out ${prefix}-busco \\
+        --mode $mode \\
+        $busco_lineage \\
+        $busco_lineage_dir \\
+        $busco_config \\
+        $args
+
+    # clean up
+    rm -rf "\$INPUT_SEQS"
+
+    # Move files to avoid staging/publishing issues
+    mv ${prefix}-busco/batch_summary.txt ${prefix}-busco.batch_summary.txt
+    mv ${prefix}-busco/*/short_summary.*.{json,txt} . || echo "Short summaries were not available: No genes were found."
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' )
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix      = task.ext.prefix ?: "${meta.id}-${lineage}"
+    def fasta_name  = files(fasta).first().name - '.gz'
+    """
+    touch ${prefix}-busco.batch_summary.txt
+    mkdir -p ${prefix}-busco/$fasta_name/run_${lineage}/busco_sequences
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/busco/busco/meta.yml b/modules/pfr/busco/busco/meta.yml
new file mode 100644
index 00000000..29745d2c
--- /dev/null
+++ b/modules/pfr/busco/busco/meta.yml
@@ -0,0 +1,98 @@
+name: busco_busco
+description: Benchmarking Universal Single Copy Orthologs
+keywords:
+  - quality control
+  - genome
+  - transcriptome
+  - proteome
+tools:
+  - busco:
+      description: BUSCO provides measures for quantitative assessment of genome assembly, gene set, and transcriptome completeness based on evolutionarily informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB.
+      homepage: https://busco.ezlab.org/
+      documentation: https://busco.ezlab.org/busco_userguide.html
+      tool_dev_url: https://gitlab.com/ezlab/busco
+      doi: "10.1007/978-1-4939-9173-0_14"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fasta:
+      type: file
+      description: Nucleic or amino acid sequence file in FASTA format.
+      pattern: "*.{fasta,fna,fa,fasta.gz,fna.gz,fa.gz}"
+  - mode:
+      type: string
+      description: The mode to run Busco in. One of genome, proteins, or transcriptome
+      pattern: "{genome,proteins,transcriptome}"
+  - lineage:
+      type: string
+      description: The BUSCO lineage to use, or "auto" to automatically select lineage
+  - busco_lineages_path:
+      type: directory
+      description: Path to local BUSCO lineages directory.
+  - config_file:
+      type: file
+      description: Path to BUSCO config file.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - batch_summary:
+      type: file
+      description: Summary of all sequence files analyzed
+      pattern: "*-busco.batch_summary.txt"
+  - short_summaries_txt:
+      type: file
+      description: Short Busco summary in plain text format
+      pattern: "short_summary.*.txt"
+  - short_summaries_json:
+      type: file
+      description: Short Busco summary in JSON format
+      pattern: "short_summary.*.json"
+  - busco_dir:
+      type: directory
+      description: BUSCO lineage specific output
+      pattern: "*-busco"
+  - full_table:
+      type: file
+      description: Full BUSCO results table
+      pattern: "full_table.tsv"
+  - missing_busco_list:
+      type: file
+      description: List of missing BUSCOs
+      pattern: "missing_busco_list.tsv"
+  - single_copy_proteins:
+      type: file
+      description: Fasta file of single copy proteins (transcriptome mode)
+      pattern: "single_copy_proteins.faa"
+  - seq_dir:
+      type: directory
+      description: BUSCO sequence directory
+      pattern: "busco_sequences"
+  - translated_dir:
+      type: directory
+      description: Six frame translations of each transcript made by the transcriptome mode
+      pattern: "translated_dir"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@priyanka-surana"
+  - "@charles-plessy"
+  - "@mahesh-panchal"
+  - "@muffato"
+  - "@jvhagey"
+  - "@gallvp"
+maintainers:
+  - "@priyanka-surana"
+  - "@charles-plessy"
+  - "@mahesh-panchal"
+  - "@muffato"
+  - "@jvhagey"
+  - "@gallvp"
diff --git a/modules/pfr/busco/busco/tests/main.nf.test b/modules/pfr/busco/busco/tests/main.nf.test
new file mode 100644
index 00000000..16b708b2
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/main.nf.test
@@ -0,0 +1,419 @@
+nextflow_process {
+
+    name "Test Process BUSCO_BUSCO"
+    script "../main.nf"
+    process "BUSCO_BUSCO"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "busco"
+    tag "busco/busco"
+
+    test("test_busco_genome_single_fasta") {
+
+        config './nextflow.config'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file( params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                ]
+                input[1] = 'genome'
+                input[2] = 'bacteria_odb10' // Launch with 'auto' to use --auto-lineage, and specified lineages // 'auto' removed from test due to memory issues
+                input[3] = [] // Download busco lineage
+                input[4] = [] // No config
+                """
+            }
+        }
+
+        then {
+            assert process.success
+
+            with(path(process.out.short_summaries_txt[0][1]).text) {
+                assert contains('BUSCO version')
+                assert contains('The lineage dataset is')
+                assert contains('BUSCO was run in mode')
+                assert contains('Complete BUSCOs')
+                assert contains('Missing BUSCOs')
+                assert contains('Dependencies and versions')
+            }
+
+            with(path(process.out.short_summaries_json[0][1]).text) {
+                assert contains('one_line_summary')
+                assert contains('mode')
+                assert contains('dataset')
+            }
+
+            assert snapshot(
+                    process.out.batch_summary[0][1],
+                    process.out.full_table[0][1],
+                    process.out.missing_busco_list[0][1],
+                    process.out.versions[0]
+                ).match()
+
+            with(file(process.out.seq_dir[0][1]).listFiles().collect { it.name }) {
+                assert contains('single_copy_busco_sequences.tar.gz')
+                assert contains('multi_copy_busco_sequences.tar.gz')
+                assert contains('fragmented_busco_sequences.tar.gz')
+            }
+
+            with(path("${process.out.busco_dir[0][1]}/logs/busco.log").text) {
+                assert contains('DEBUG:busco.run_BUSCO')
+                assert contains('Results from dataset')
+                assert contains('how to cite BUSCO')
+            }
+
+            assert process.out.single_copy_proteins == []
+            assert process.out.translated_dir == []
+        }
+    }
+
+    test("test_busco_genome_multi_fasta") {
+
+        config './nextflow.config'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    [
+                        file( params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true),
+                        file( params.test_data['candidatus_portiera_aleyrodidarum']['genome']['genome_fasta'], checkIfExists: true)
+                    ]
+                ]
+                input[1] = 'genome'
+                input[2] = 'bacteria_odb10'
+                input[3] = []
+                input[4] = []
+                """
+            }
+        }
+
+        then {
+            assert process.success
+
+            with(path(process.out.short_summaries_txt[0][1][0]).text) {
+                assert contains('BUSCO version')
+                assert contains('The lineage dataset is')
+                assert contains('BUSCO was run in mode')
+                assert contains('Complete BUSCOs')
+                assert contains('Missing BUSCOs')
+                assert contains('Dependencies and versions')
+            }
+
+            with(path(process.out.short_summaries_txt[0][1][1]).text) {
+                assert contains('BUSCO version')
+                assert contains('The lineage dataset is')
+                assert contains('BUSCO was run in mode')
+                assert contains('Complete BUSCOs')
+                assert contains('Missing BUSCOs')
+                assert contains('Dependencies and versions')
+            }
+
+            with(path(process.out.short_summaries_json[0][1][0]).text) {
+                assert contains('one_line_summary')
+                assert contains('mode')
+                assert contains('dataset')
+            }
+
+            with(path(process.out.short_summaries_json[0][1][1]).text) {
+                assert contains('one_line_summary')
+                assert contains('mode')
+                assert contains('dataset')
+            }
+
+            assert snapshot(
+                    process.out.batch_summary[0][1],
+                    process.out.full_table[0][1],
+                    process.out.missing_busco_list[0][1],
+                    process.out.versions[0]
+                ).match()
+
+            with(file(process.out.seq_dir[0][1][0]).listFiles().collect { it.name }) {
+                assert contains('single_copy_busco_sequences.tar.gz')
+                assert contains('multi_copy_busco_sequences.tar.gz')
+                assert contains('fragmented_busco_sequences.tar.gz')
+            }
+
+            with(file(process.out.seq_dir[0][1][1]).listFiles().collect { it.name }) {
+                assert contains('single_copy_busco_sequences.tar.gz')
+                assert contains('multi_copy_busco_sequences.tar.gz')
+                assert contains('fragmented_busco_sequences.tar.gz')
+            }
+
+            with(path("${process.out.busco_dir[0][1]}/logs/busco.log").text) {
+                assert contains('DEBUG:busco.run_BUSCO')
+                assert contains('Results from dataset')
+                assert contains('how to cite BUSCO')
+            }
+
+            assert process.out.single_copy_proteins == []
+            assert process.out.translated_dir == []
+        }
+
+    }
+
+    test("test_busco_eukaryote_metaeuk") {
+
+        config './nextflow.metaeuk.config'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file( params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = 'genome'
+                input[2] = 'eukaryota_odb10'
+                input[3] = []
+                input[4] = []
+                """
+            }
+        }
+
+        then {
+            assert process.success
+
+            with(path(process.out.short_summaries_txt[0][1]).text) {
+                assert contains('BUSCO version')
+                assert contains('The lineage dataset is')
+                assert contains('BUSCO was run in mode')
+                assert contains('Complete BUSCOs')
+                assert contains('Missing BUSCOs')
+                assert contains('Dependencies and versions')
+            }
+
+            with(path(process.out.short_summaries_json[0][1]).text) {
+                assert contains('one_line_summary')
+                assert contains('mode')
+                assert contains('dataset')
+            }
+
+            assert snapshot(
+                    process.out.batch_summary[0][1],
+                    process.out.full_table[0][1],
+                    process.out.missing_busco_list[0][1],
+                    process.out.versions[0]
+                ).match()
+
+            with(file(process.out.seq_dir[0][1]).listFiles().collect { it.name }) {
+                assert contains('single_copy_busco_sequences.tar.gz')
+                assert contains('multi_copy_busco_sequences.tar.gz')
+                assert contains('fragmented_busco_sequences.tar.gz')
+            }
+
+            with(path("${process.out.busco_dir[0][1]}/logs/busco.log").text) {
+                assert contains('DEBUG:busco.run_BUSCO')
+                assert contains("'use_augustus', 'False'")
+                assert contains("'use_metaeuk', 'True'") // METAEUK
+                assert contains('Results from dataset')
+                assert contains('how to cite BUSCO')
+
+            }
+
+            assert process.out.single_copy_proteins == []
+            assert process.out.translated_dir == []
+        }
+
+    }
+
+    test("test_busco_eukaryote_augustus") {
+
+        config './nextflow.augustus.config'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file( params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = 'genome'
+                input[2] = 'eukaryota_odb10'
+                input[3] = []
+                input[4] = []
+                """
+            }
+        }
+
+        then {
+            assert process.success
+
+            assert snapshot(
+                    process.out.batch_summary[0][1],
+                    process.out.versions[0]
+                ).match()
+
+            with(path("${process.out.busco_dir[0][1]}/logs/busco.log").text) {
+                assert contains('DEBUG:busco.run_BUSCO')
+                assert contains("'use_augustus', 'True'")
+                assert contains("'use_metaeuk', 'False'") // AUGUSTUS
+                assert contains('Augustus did not recognize any genes')
+
+            }
+
+            assert process.out.short_summaries_json == []
+            assert process.out.short_summaries_txt == []
+            assert process.out.missing_busco_list == []
+            assert process.out.full_table == []
+            assert process.out.single_copy_proteins == []
+            assert process.out.translated_dir == []
+        }
+
+    }
+
+    test("test_busco_protein") {
+
+        config './nextflow.config'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file( params.test_data['candidatus_portiera_aleyrodidarum']['genome']['proteome_fasta'], checkIfExists: true)
+                ]
+                input[1] = 'proteins'
+                input[2] = 'bacteria_odb10'
+                input[3] = []
+                input[4] = []
+                """
+            }
+        }
+
+        then {
+            assert process.success
+
+            with(path(process.out.short_summaries_txt[0][1]).text) {
+                assert contains('BUSCO version')
+                assert contains('The lineage dataset is')
+                assert contains('BUSCO was run in mode')
+                assert contains('Complete BUSCOs')
+                assert contains('Missing BUSCOs')
+                assert contains('Dependencies and versions')
+            }
+
+            with(path(process.out.short_summaries_json[0][1]).text) {
+                assert contains('one_line_summary')
+                assert contains('mode')
+                assert contains('dataset')
+            }
+
+            assert snapshot(
+                    process.out.batch_summary[0][1],
+                    process.out.full_table[0][1],
+                    process.out.missing_busco_list[0][1],
+                    process.out.versions[0]
+                ).match()
+
+            with(file(process.out.seq_dir[0][1]).listFiles().collect { it.name }) {
+                assert contains('single_copy_busco_sequences.tar.gz')
+                assert contains('multi_copy_busco_sequences.tar.gz')
+                assert contains('fragmented_busco_sequences.tar.gz')
+            }
+
+            with(path("${process.out.busco_dir[0][1]}/logs/busco.log").text) {
+                assert contains('DEBUG:busco.run_BUSCO')
+                assert contains('Results from dataset')
+                assert contains('how to cite BUSCO')
+            }
+
+            assert process.out.single_copy_proteins == []
+            assert process.out.translated_dir == []
+        }
+
+    }
+
+    test("test_busco_transcriptome") {
+
+        config './nextflow.config'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file( params.test_data['bacteroides_fragilis']['illumina']['test1_contigs_fa_gz'], checkIfExists: true)
+                ]
+                input[1] = 'transcriptome'
+                input[2] = 'bacteria_odb10'
+                input[3] = []
+                input[4] = []
+                """
+            }
+        }
+
+        then {
+            assert process.success
+
+            with(path(process.out.short_summaries_txt[0][1]).text) {
+                assert contains('BUSCO version')
+                assert contains('The lineage dataset is')
+                assert contains('BUSCO was run in mode')
+                assert contains('Complete BUSCOs')
+                assert contains('Missing BUSCOs')
+                assert contains('Dependencies and versions')
+            }
+
+            with(path(process.out.short_summaries_json[0][1]).text) {
+                assert contains('one_line_summary')
+                assert contains('mode')
+                assert contains('dataset')
+            }
+
+            assert snapshot(
+                    process.out.batch_summary[0][1],
+                    process.out.full_table[0][1],
+                    process.out.missing_busco_list[0][1],
+                    process.out.translated_dir[0][1],
+                    process.out.single_copy_proteins[0][1],
+                    process.out.versions[0]
+                ).match()
+
+            with(file(process.out.seq_dir[0][1]).listFiles().collect { it.name }) {
+                assert contains('single_copy_busco_sequences.tar.gz')
+                assert contains('multi_copy_busco_sequences.tar.gz')
+                assert contains('fragmented_busco_sequences.tar.gz')
+            }
+
+            with(path("${process.out.busco_dir[0][1]}/logs/busco.log").text) {
+                assert contains('DEBUG:busco.run_BUSCO')
+                assert contains('Results from dataset')
+                assert contains('how to cite BUSCO')
+            }
+        }
+
+    }
+
+    test("minimal-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file( params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                ]
+                input[1] = 'genome'
+                input[2] = 'bacteria_odb10'
+                input[3] = []
+                input[4] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/pfr/busco/busco/tests/main.nf.test.snap b/modules/pfr/busco/busco/tests/main.nf.test.snap
new file mode 100644
index 00000000..1b6411bc
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/main.nf.test.snap
@@ -0,0 +1,230 @@
+{
+    "minimal-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "7": [
+                    
+                ],
+                "8": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            [
+                                [
+                                    [
+                                        
+                                    ]
+                                ]
+                            ]
+                        ]
+                    ]
+                ],
+                "9": [
+                    "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+                ],
+                "batch_summary": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "busco_dir": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            [
+                                [
+                                    [
+                                        
+                                    ]
+                                ]
+                            ]
+                        ]
+                    ]
+                ],
+                "full_table": [
+                    
+                ],
+                "missing_busco_list": [
+                    
+                ],
+                "seq_dir": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "short_summaries_json": [
+                    
+                ],
+                "short_summaries_txt": [
+                    
+                ],
+                "single_copy_proteins": [
+                    
+                ],
+                "translated_dir": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:28:04.451297"
+    },
+    "test_busco_eukaryote_augustus": {
+        "content": [
+            "test-eukaryota_odb10-busco.batch_summary.txt:md5,3ea3bdc423a461dae514d816bdc61c89",
+            "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:26:36.974986"
+    },
+    "test_busco_genome_single_fasta": {
+        "content": [
+            "test-bacteria_odb10-busco.batch_summary.txt:md5,21b3fb771cf36be917cc451540d999be",
+            "full_table.tsv:md5,638fe7590f442c57361554dae330eca1",
+            "missing_busco_list.tsv:md5,1530af4fe7673a6d001349537bcd410a",
+            "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:22:45.07816"
+    },
+    "test_busco_genome_multi_fasta": {
+        "content": [
+            "test-bacteria_odb10-busco.batch_summary.txt:md5,fcd3c208913e8abda3d6742c43fec5fa",
+            [
+                "full_table.tsv:md5,c657edcc7d0de0175869717551df6e83",
+                "full_table.tsv:md5,638fe7590f442c57361554dae330eca1"
+            ],
+            [
+                "missing_busco_list.tsv:md5,aceb66e347a353cb7fca8e2a725f9112",
+                "missing_busco_list.tsv:md5,1530af4fe7673a6d001349537bcd410a"
+            ],
+            "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:23:50.255602"
+    },
+    "test_busco_eukaryote_metaeuk": {
+        "content": [
+            "test-eukaryota_odb10-busco.batch_summary.txt:md5,ff6d8277e452a83ce9456bbee666feb6",
+            "full_table.tsv:md5,92b1b1d5cb5ea0e2093d16f00187e8c7",
+            "missing_busco_list.tsv:md5,0352e563de290bf804c708323c35a9e3",
+            "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:25:38.159041"
+    },
+    "test_busco_transcriptome": {
+        "content": [
+            "test-bacteria_odb10-busco.batch_summary.txt:md5,8734b3f379c4c0928e5dd4ea1873dc64",
+            "full_table.tsv:md5,1b2ce808fdafa744c56b5f781551272d",
+            "missing_busco_list.tsv:md5,a6931b6470262b997b8b99ea0f1d14a4",
+            [
+                "1024388at2.faa:md5,797d603d262a6595a112e25b73e878b0",
+                "1054741at2.faa:md5,cd4b928cba6b19b4437746ba507e7195",
+                "1093223at2.faa:md5,df9549708e5ffcfaee6a74dd70a0e5dc",
+                "1151822at2.faa:md5,12726afc1cdc40c13392e1596e93df3a",
+                "143460at2.faa:md5,d887431fd988a5556a523440f02d9594",
+                "1491686at2.faa:md5,d03362d19979b27306c192f1c74a84e5",
+                "1504821at2.faa:md5,4f5f6e5c57bac0092c1d85ded73d7e67",
+                "1574817at2.faa:md5,1153e55998c2929eacad2aed7d08d248",
+                "1592033at2.faa:md5,bb7a59e5f3a57ba12d10dabf4c77ab57",
+                "1623045at2.faa:md5,8fe38155feb1802beb97ef7714837bf5",
+                "1661836at2.faa:md5,6c6d592c2fbb0d7a4e5e1f47a15644f0",
+                "1674344at2.faa:md5,bb41b44e53565a54cadf0b780532fe08",
+                "1698718at2.faa:md5,f233860000028eb00329aa85236c71e5",
+                "1990650at2.faa:md5,34a2d29c5f8b6253159ddb7a43fa1829",
+                "223233at2.faa:md5,dec6705c7846c989296e73942f953cbc",
+                "402899at2.faa:md5,acc0f271f9a586d2ce1ee41669b22999",
+                "505485at2.faa:md5,aa0391f8fa5d9bd19b30d844d5a99845",
+                "665824at2.faa:md5,47f8ad43b6a6078206feb48c2e552793",
+                "776861at2.faa:md5,f8b90c13f7c6be828dea3bb920195e3d",
+                "874197at2.faa:md5,8d22a35a768debe6f376fc695d233a69",
+                "932854at2.faa:md5,2eff2de1ab83b22f3234a529a44e22bb",
+                "95696at2.faa:md5,247bfd1aef432f7b5456307768e9149c"
+            ],
+            "single_copy_proteins.faa:md5,73e2c5d6a9b0f01f2deea3cc5f21b764",
+            "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:27:53.992893"
+    },
+    "test_busco_protein": {
+        "content": [
+            "test-bacteria_odb10-busco.batch_summary.txt:md5,f5a782378f9f94a748aa907381fdef91",
+            "full_table.tsv:md5,812ab6a0496fccab774643cf40c4f2a8",
+            "missing_busco_list.tsv:md5,aceb66e347a353cb7fca8e2a725f9112",
+            "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T13:27:12.724862"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/busco/busco/tests/nextflow.augustus.config b/modules/pfr/busco/busco/tests/nextflow.augustus.config
new file mode 100644
index 00000000..84daa69d
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/nextflow.augustus.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'BUSCO_BUSCO' {
+        ext.args = '--tar --augustus'
+    }
+}
diff --git a/modules/pfr/busco/busco/tests/nextflow.config b/modules/pfr/busco/busco/tests/nextflow.config
new file mode 100644
index 00000000..1ec3fec0
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'BUSCO_BUSCO' {
+        ext.args = '--tar'
+    }
+}
diff --git a/modules/pfr/busco/busco/tests/nextflow.metaeuk.config b/modules/pfr/busco/busco/tests/nextflow.metaeuk.config
new file mode 100644
index 00000000..c1418445
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/nextflow.metaeuk.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'BUSCO_BUSCO' {
+        ext.args = '--tar --metaeuk'
+    }
+}
diff --git a/modules/pfr/busco/busco/tests/old_test.yml b/modules/pfr/busco/busco/tests/old_test.yml
new file mode 100644
index 00000000..75177f5d
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/old_test.yml
@@ -0,0 +1,624 @@
+- name: busco test_busco_genome_single_fasta
+  command: nextflow run ./tests/modules/nf-core/busco -entry test_busco_genome_single_fasta -c ./tests/config/nextflow.config
+  tags:
+    - busco
+  files:
+    - path: output/busco/short_summary.specific.bacteria_odb10.genome.fna.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.bacteria_odb10.genome.fna.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/test-bacteria_odb10-busco.batch_summary.txt
+      md5sum: bc2440f8a68d7fbf931ff911c1c3fdfa
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/bbtools_err.log
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/bbtools_out.log
+      md5sum: 9caf1a1434414c78562eb0bbb9c0e53f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/hmmsearch_err.log
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/hmmsearch_out.log
+      contains:
+        - "# hmmsearch :: search profile(s) against a sequence database"
+        - "# target sequence database:"
+        - "Internal pipeline statistics summary:"
+        - "[ok]"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/prodigal_err.log
+      md5sum: 538510cfc7483498210f01e53fe035ad
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/prodigal_out.log
+      md5sum: 61050b0706addc9498b2088a2d6efa9a
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/.checkpoint
+      contains:
+        - "Tool: prodigal"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/predicted.faa
+      md5sum: 836e9a80d33d8b89168f07ddc13ee991
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/predicted.fna
+      md5sum: 20eeb75f86842e6e136f02bca8b73a9f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11.faa
+      md5sum: 836e9a80d33d8b89168f07ddc13ee991
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11.fna
+      md5sum: 20eeb75f86842e6e136f02bca8b73a9f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11_err.log
+      md5sum: 538510cfc7483498210f01e53fe035ad
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11_out.log
+      md5sum: 61050b0706addc9498b2088a2d6efa9a
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/.bbtools_output/.checkpoint
+      contains:
+        - "Tool: bbtools"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/full_table.tsv
+      md5sum: c56edab1dc1522e993c25ae2b730799f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/missing_busco_list.tsv
+      md5sum: b533ef30270f27160acce85a22d01bf5
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "lineage_dataset"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/test-bacteria_odb10-busco/logs/busco.log
+      contains:
+        - "DEBUG:busco.run_BUSCO"
+        - "Results from dataset"
+        - "how to cite BUSCO"
+    - path: output/busco/versions.yml
+
+- name: busco test_busco_genome_multi_fasta
+  command: nextflow run ./tests/modules/nf-core/busco -entry test_busco_genome_multi_fasta -c ./tests/config/nextflow.config
+  tags:
+    - busco
+  files:
+    - path: output/busco/short_summary.specific.bacteria_odb10.genome.fasta.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.bacteria_odb10.genome.fasta.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/short_summary.specific.bacteria_odb10.genome.fna.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.bacteria_odb10.genome.fna.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/test-bacteria_odb10-busco.batch_summary.txt
+      md5sum: 8c64c1a28b086ef2ee444f99cbed5f7d
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/logs/bbtools_err.log
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/logs/bbtools_out.log
+      md5sum: 8f047bdb33264d22a83920bc2c63f29a
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/logs/hmmsearch_err.log
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/logs/hmmsearch_out.log
+      contains:
+        - "# hmmsearch :: search profile(s) against a sequence database"
+        - "# target sequence database:"
+        - "Internal pipeline statistics summary:"
+        - "[ok]"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/logs/prodigal_err.log
+      md5sum: c1fdc6977332f53dfe7f632733bb4585
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/logs/prodigal_out.log
+      md5sum: 50752acb1c5a20be886bfdfc06635bcb
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/.checkpoint
+      contains:
+        - "Tool: prodigal"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/predicted.faa
+      md5sum: 8166471fc5f08c82fd5643ab42327f9d
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/predicted.fna
+      md5sum: ddc508a18f60e7f3314534df50cdf8ca
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11.faa
+      md5sum: 8166471fc5f08c82fd5643ab42327f9d
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11.fna
+      md5sum: ddc508a18f60e7f3314534df50cdf8ca
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11_err.log
+      md5sum: c1fdc6977332f53dfe7f632733bb4585
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11_out.log
+      md5sum: 50752acb1c5a20be886bfdfc06635bcb
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_4.faa
+      md5sum: e56fd59c38248dc21ac94355dca98121
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_4.fna
+      md5sum: b365f84bf99c68357952e0b98ed7ce42
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_4_err.log
+      md5sum: e5f14d7925ba14a0f9850542f3739894
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_4_out.log
+      md5sum: d41971bfc1b621d4ffd2633bc47017ea
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/.bbtools_output/.checkpoint
+      contains:
+        - "Tool: bbtools"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/full_table.tsv
+      md5sum: c9651b88b10871abc260ee655898e828
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/missing_busco_list.tsv
+      md5sum: 9939309df2da5419de88c32d1435c779
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fasta/run_bacteria_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/bbtools_err.log
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/bbtools_out.log
+      md5sum: 9caf1a1434414c78562eb0bbb9c0e53f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/hmmsearch_err.log
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/hmmsearch_out.log
+      contains:
+        - "# hmmsearch :: search profile(s) against a sequence database"
+        - "# target sequence database:"
+        - "Internal pipeline statistics summary:"
+        - "[ok]"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/prodigal_err.log
+      md5sum: 538510cfc7483498210f01e53fe035ad
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/logs/prodigal_out.log
+      md5sum: 61050b0706addc9498b2088a2d6efa9a
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/.checkpoint
+      contains:
+        - "Tool: prodigal"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/predicted.faa
+      md5sum: 836e9a80d33d8b89168f07ddc13ee991
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/predicted.fna
+      md5sum: 20eeb75f86842e6e136f02bca8b73a9f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11.faa
+      md5sum: 836e9a80d33d8b89168f07ddc13ee991
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11.fna
+      md5sum: 20eeb75f86842e6e136f02bca8b73a9f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11_err.log
+      md5sum: 538510cfc7483498210f01e53fe035ad
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/prodigal_output/predicted_genes/tmp/prodigal_mode_single_code_11_out.log
+      md5sum: 61050b0706addc9498b2088a2d6efa9a
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/.bbtools_output/.checkpoint
+      contains:
+        - "Tool: bbtools"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/full_table.tsv
+      md5sum: c56edab1dc1522e993c25ae2b730799f
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/missing_busco_list.tsv
+      md5sum: b533ef30270f27160acce85a22d01bf5
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/test-bacteria_odb10-busco/genome.fna/run_bacteria_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/test-bacteria_odb10-busco/logs/busco.log
+      contains:
+        - "DEBUG:busco.run_BUSCO"
+        - "Results from dataset"
+        - "how to cite BUSCO"
+    - path: output/busco/versions.yml
+
+- name: busco test_busco_eukaryote_metaeuk
+  command: nextflow run ./tests/modules/nf-core/busco -entry test_busco_eukaryote_metaeuk -c ./tests/config/nextflow.config
+  tags:
+    - busco
+  files:
+    - path: output/busco/short_summary.specific.eukaryota_odb10.genome.fasta.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.eukaryota_odb10.genome.fasta.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/test-eukaryota_odb10-busco.batch_summary.txt
+      md5sum: ff6d8277e452a83ce9456bbee666feb6
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/bbtools_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/bbtools_out.log
+      md5sum: e63debaa653f18f7405d936050abc093
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/hmmsearch_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/hmmsearch_out.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run1_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run1_out.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run2_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run2_out.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/.bbtools_output/.checkpoint
+      contains:
+        - "Tool: bbtools"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/full_table.tsv
+      md5sum: bd880e90b9e5620a58943a3e0f9ff16b
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/.checkpoint
+      contains:
+        - "Tool: metaeuk"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/combined_pred_proteins.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.codon.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.gff
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.headersMap.tsv
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/refseq_db_rerun.faa
+      md5sum: d80b8fa4cb5ed0d47d63d6aa93635bc2
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.codon.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.gff
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.headersMap.tsv
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/missing_busco_list.tsv
+      md5sum: 1e8e79c540fd2e69ba0d2659d9eb2988
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/test-eukaryota_odb10-busco/logs/busco.log
+      contains:
+        - "DEBUG:busco.run_BUSCO"
+        - "Results from dataset"
+        - "how to cite BUSCO"
+    - path: output/busco/versions.yml
+
+- name: busco test_busco_eukaryote_augustus
+  command: nextflow run ./tests/modules/nf-core/busco -entry test_busco_eukaryote_augustus -c ./tests/config/nextflow.config
+  tags:
+    - busco
+  files:
+    - path: output/busco/short_summary.specific.eukaryota_odb10.genome.fasta.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.eukaryota_odb10.genome.fasta.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/test-eukaryota_odb10-busco.batch_summary.txt
+      md5sum: ff6d8277e452a83ce9456bbee666feb6
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/bbtools_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/bbtools_out.log
+      md5sum: e63debaa653f18f7405d936050abc093
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/hmmsearch_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/hmmsearch_out.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run1_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run1_out.log
+      contains:
+        - "metaeuk"
+        - "easy-predict"
+        - "Compute score and coverage"
+        - "Time for processing:"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run2_err.log
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/logs/metaeuk_run2_out.log
+      contains:
+        - "metaeuk"
+        - "easy-predict"
+        - "Compute score and coverage"
+        - "Time for processing:"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/.bbtools_output/.checkpoint
+      contains:
+        - "Tool: bbtools"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/full_table.tsv
+      md5sum: bd880e90b9e5620a58943a3e0f9ff16b
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/.checkpoint
+      contains:
+        - "Tool: metaeuk"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/combined_pred_proteins.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.codon.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.gff
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/initial_results/genome.fasta.headersMap.tsv
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/refseq_db_rerun.faa
+      md5sum: d80b8fa4cb5ed0d47d63d6aa93635bc2
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.codon.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.fas
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.gff
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/metaeuk_output/rerun_results/genome.fasta.headersMap.tsv
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/missing_busco_list.tsv
+      md5sum: 1e8e79c540fd2e69ba0d2659d9eb2988
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/test-eukaryota_odb10-busco/genome.fasta/run_eukaryota_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/test-eukaryota_odb10-busco/logs/busco.log
+      contains:
+        - "DEBUG:busco.run_BUSCO"
+        - "Results from dataset"
+        - "how to cite BUSCO"
+    - path: output/busco/versions.yml
+
+- name: busco test_busco_protein
+  command: nextflow run ./tests/modules/nf-core/busco -entry test_busco_protein -c ./tests/config/nextflow.config
+  tags:
+    - busco
+  files:
+    - path: output/busco/short_summary.specific.bacteria_odb10.proteome.fasta.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.bacteria_odb10.proteome.fasta.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/test-bacteria_odb10-busco.batch_summary.txt
+      md5sum: 7a65e6cbb6c56a2ea4e739ae0aa3297d
+    - path: output/busco/test-bacteria_odb10-busco/logs/busco.log
+      contains:
+        - "DEBUG:busco.run_BUSCO"
+        - "Results from dataset"
+        - "how to cite BUSCO"
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/logs/hmmsearch_err.log
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/logs/hmmsearch_out.log
+      contains:
+        - "# hmmsearch :: search profile(s) against a sequence database"
+        - "# target sequence database:"
+        - "Internal pipeline statistics summary:"
+        - "[ok]"
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/full_table.tsv
+      md5sum: 0e34f1011cd83ea1d5d5103ec62b8922
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/missing_busco_list.tsv
+      md5sum: 9939309df2da5419de88c32d1435c779
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/test-bacteria_odb10-busco/proteome.fasta/run_bacteria_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/versions.yml
+
+- name: busco test_busco_transcriptome
+  command: nextflow run ./tests/modules/nf-core/busco -entry test_busco_transcriptome -c ./tests/config/nextflow.config
+  tags:
+    - busco
+  files:
+    - path: output/busco/short_summary.specific.bacteria_odb10.test1.contigs.fa.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/short_summary.specific.bacteria_odb10.test1.contigs.fa.txt
+      contains:
+        - "BUSCO version"
+        - "The lineage dataset is"
+        - "BUSCO was run in mode"
+        - "Complete BUSCOs"
+        - "Missing BUSCOs"
+        - "Dependencies and versions"
+    - path: output/busco/test-bacteria_odb10-busco.batch_summary.txt
+      md5sum: 46118ecf60d1b87d22b96d80f4f03632
+    - path: output/busco/test-bacteria_odb10-busco/logs/busco.log
+      contains:
+        - "DEBUG:busco.run_BUSCO"
+        - "Results from dataset"
+        - "how to cite BUSCO"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/.checkpoint
+      contains:
+        - "Tool: makeblastdb"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.ndb
+      md5sum: 3788c017fe5e6f0f58224e9cdd21822b
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.nhr
+      md5sum: 8ecd2ce392bb5e25ddbe1d85f879582e
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.nin
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.njs
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.not
+      md5sum: 0c340e376c7e85d19f82ec1a833e6a6e
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.nsq
+      md5sum: 532d5c0a7ea00fe95ca3c97cb3be6198
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.ntf
+      md5sum: de1250813f0c7affc6d12dac9d0fb6bb
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/blast_db/test1.contigs.fa.nto
+      md5sum: ff74bd41f9cc9b011c63a32c4f7693bf
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/logs/hmmsearch_err.log
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/logs/hmmsearch_out.log
+      contains:
+        - "# hmmsearch :: search profile(s) against a sequence database"
+        - "# target sequence database:"
+        - "Internal pipeline statistics summary:"
+        - "[ok]"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/logs/makeblastdb_err.log
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/logs/makeblastdb_out.log
+      contains:
+        - "Building a new DB"
+        - "Adding sequences from FASTA"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/logs/tblastn_err.log
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/logs/tblastn_out.log
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/.checkpoint
+      contains:
+        - "Tool: tblastn"
+        - "Completed"
+        - "jobs"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/coordinates.tsv
+      md5sum: cc30eed321944af293452bdbcfc24292
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_101.temp
+      md5sum: 73e9c65fc83fedc58f57f09b08f08238
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_119.temp
+      md5sum: 7fa4cc7955ec0cc36330a221c579b975
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_129.temp
+      md5sum: 6f1601c875d019e3f6f1f98ed8e988d4
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_138.temp
+      md5sum: 3f8e034686cd240c2330650d791bcae2
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_143.temp
+      md5sum: df3dfa8e9ba30ed70cf75b5e7abf2179
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_172.temp
+      md5sum: 7d463e0e6cf7169bc9077d8dc776dda1
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_178.temp
+      md5sum: 2288edf7fa4f88f51b4cf4d94086f77e
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_188.temp
+      md5sum: 029906abbad6d87fc57830dd548cac24
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_195.temp
+      md5sum: 4937f3b348774a31b1160a00297c29cc
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_210.temp
+      md5sum: afcb20ba4c466479d6b91c8c62251e1f
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_232.temp
+      md5sum: 2e1e823ce017345bd998191a39fa9924
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_268.temp
+      md5sum: 08c2d82c34ecffbe1c638b410349412e
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_29.temp
+      md5sum: cd9b63cf93524284781535c888313764
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_44.temp
+      md5sum: d1929b742b24ebe379bf4801ca882dca
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_58.temp
+      md5sum: 69215765b010c05336538cb322c900b3
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_72.temp
+      md5sum: 6feaa1cc3b0899a147ea9d466878f3e3
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_80.temp
+      md5sum: 13625eae14e860a96ce17cd4e37e9d01
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_81.temp
+      md5sum: e14b2484649b0dbc8926815c207b806d
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_93.temp
+      md5sum: 6902c93691df00e690faea914c71839e
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/sequences/k141_97.temp
+      md5sum: 0a0d9d38a83acbd5ad43c29cdf429988
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/blast_output/tblastn.tsv
+      contains:
+        - "TBLASTN"
+        - "BLAST processed"
+        - "queries"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/busco_sequences/fragmented_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/busco_sequences/multi_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/busco_sequences/single_copy_busco_sequences.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/full_table.tsv
+      md5sum: 24df25199e13c88bd892fc3e7b541ca0
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/hmmer_output.tar.gz
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/missing_busco_list.tsv
+      md5sum: e7232e2b8cca4fdfdd9e363b39ebbc81
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/short_summary.json
+      contains:
+        - "one_line_summary"
+        - "mode"
+        - "dataset"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/short_summary.txt
+      contains:
+        - "# BUSCO version is:"
+        - "Results:"
+        - "busco:"
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/run_bacteria_odb10/single_copy_proteins.faa
+      md5sum: e04b9465733577ae6e4bccb7aa01e720
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1024388at2.faa
+      md5sum: 7333c39a20258f20c7019ea0cd83157c
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1054741at2.faa
+      md5sum: ebb481e77a824685fbe04d8a2f3a0d7d
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1093223at2.faa
+      md5sum: 34621c7d499034e8f8e6b92fd4020a93
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1151822at2.faa
+      md5sum: aa89ca381c1c70c9c4e1380351ca7c2a
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/143460at2.faa
+      md5sum: f2e91d78b8dd3722840378789f29e8c8
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1491686at2.faa
+      md5sum: 73c25aef5c9cba7f4151804941b146ea
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1504821at2.faa
+      md5sum: cda556018d1f84ebe517e89f6fc107d0
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1574817at2.faa
+      md5sum: a9096c9fb8b25c78a72871ab0463acdc
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1592033at2.faa
+      md5sum: e463d25ce186c0cebfd749474f3a4c64
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1623045at2.faa
+      md5sum: f2cfd241590c6d8377286d6135480937
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1661836at2.faa
+      md5sum: 586569546fb9861502468e3d9ba2775c
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1674344at2.faa
+      md5sum: 24c658bee14ad84b062d81ad96642eb8
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1698718at2.faa
+      md5sum: 0b8e26ddf5149bbd8805be7af125208d
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/1990650at2.faa
+      md5sum: 159320712ee01fb2ccb31a25df44eead
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/223233at2.faa
+      md5sum: 812629c0b06ac3d18661c2ca78de0c08
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/402899at2.faa
+      md5sum: f7ff4e1591342d30b77392a2e84b57d9
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/505485at2.faa
+      md5sum: 7b34a24fc49c540d46fcf96ff5129564
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/665824at2.faa
+      md5sum: 4cff2df64f6bcaff8bc19c234c8bcccd
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/776861at2.faa
+      md5sum: 613af7a3fea30ea2bece66f603b9284a
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/874197at2.faa
+      md5sum: a7cd1b13c9ef91c7ef4e31614166f197
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/932854at2.faa
+      md5sum: fe313ffd5efdb0fed887a04fba352552
+    - path: output/busco/test-bacteria_odb10-busco/test1.contigs.fa/translated_proteins/95696at2.faa
+      md5sum: 4e1f30a2fea4dfbf9bb7fae2700622a0
+    - path: output/busco/versions.yml
diff --git a/modules/pfr/busco/busco/tests/tags.yml b/modules/pfr/busco/busco/tests/tags.yml
new file mode 100644
index 00000000..7c4d2835
--- /dev/null
+++ b/modules/pfr/busco/busco/tests/tags.yml
@@ -0,0 +1,2 @@
+busco/busco:
+  - "modules/nf-core/busco/busco/**"
diff --git a/modules/pfr/busco/generateplot/environment.yml b/modules/pfr/busco/generateplot/environment.yml
new file mode 100644
index 00000000..1ca5babb
--- /dev/null
+++ b/modules/pfr/busco/generateplot/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "busco_generateplot"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::busco=5.7.1
diff --git a/modules/local/busco_plot.nf b/modules/pfr/busco/generateplot/main.nf
similarity index 54%
rename from modules/local/busco_plot.nf
rename to modules/pfr/busco/generateplot/main.nf
index e8f70189..6a4b3393 100644
--- a/modules/local/busco_plot.nf
+++ b/modules/pfr/busco/generateplot/main.nf
@@ -1,26 +1,30 @@
-process BUSCO_PLOT {
-    tag 'all summaries'
+process BUSCO_GENERATEPLOT {
     label 'process_single'
 
-    conda "bioconda::busco=5.6.1"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/busco:5.6.1--pyhdfd78af_0':
-        'biocontainers/busco:5.6.1--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/busco:5.7.1--pyhdfd78af_0':
+        'biocontainers/busco:5.7.1--pyhdfd78af_0' }"
 
     input:
-    path "short_summary.*", stageAs: 'busco/*'
+    path short_summary_txt, stageAs: 'busco/*'
 
     output:
-    path 'busco/*.png'  , emit: png
+    path '*.png'        , emit: png
     path "versions.yml" , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
+    def args    = task.ext.args     ?: ''
+    def prefix  = task.ext.prefix   ?: 'busco_figure'
     """
     generate_plot.py \\
-        -wd ./busco
+        $args \\
+        -wd busco
+
+    mv ./busco/busco_figure.png ${prefix}.png
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -29,10 +33,9 @@ process BUSCO_PLOT {
     """
 
     stub:
+    def prefix  = task.ext.prefix   ?: 'busco_figure'
     """
-    mkdir -p busco
-
-    touch busco/summary_plot.png
+    touch ${prefix}.png
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/pfr/busco/generateplot/meta.yml b/modules/pfr/busco/generateplot/meta.yml
new file mode 100644
index 00000000..796f32b4
--- /dev/null
+++ b/modules/pfr/busco/generateplot/meta.yml
@@ -0,0 +1,35 @@
+name: "busco_generateplot"
+description: BUSCO plot generation tool
+keywords:
+  - genome
+  - fasta
+  - annotation
+  - busco
+  - transcriptome
+  - quality control
+tools:
+  - busco:
+      description: BUSCO provides measures for quantitative assessment of genome assembly, gene set, and transcriptome completeness based on evolutionarily informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB.
+      homepage: https://busco.ezlab.org/
+      documentation: https://busco.ezlab.org/busco_userguide.html
+      tool_dev_url: https://gitlab.com/ezlab/busco
+      doi: "10.1007/978-1-4939-9173-0_14"
+      licence: ["MIT"]
+input:
+  - short_summary_txt:
+      type: file
+      description: One or more short summary txt files from BUSCO
+      pattern: "short_summary.*.txt"
+output:
+  - png:
+      type: file
+      description: A summary plot in png format
+      pattern: "*.png"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/modules/pfr/busco/generateplot/tests/main.nf.test b/modules/pfr/busco/generateplot/tests/main.nf.test
new file mode 100644
index 00000000..853dc5ed
--- /dev/null
+++ b/modules/pfr/busco/generateplot/tests/main.nf.test
@@ -0,0 +1,72 @@
+nextflow_process {
+
+    name "Test Process BUSCO_GENERATEPLOT"
+    script "../main.nf"
+    process "BUSCO_GENERATEPLOT"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "busco"
+    tag "busco/busco"
+    tag "busco/generateplot"
+
+    test("bacteroides_fragilis-genome_fna_gz") {
+
+        setup {
+            run("BUSCO_BUSCO") {
+                script "../../busco"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'test' ], // meta map
+                        file(params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                    ]
+                    input[1] = 'genome'
+                    input[2] = 'bacteria_odb10'
+                    input[3] = []
+                    input[4] = []
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = BUSCO_BUSCO.out.short_summaries_txt.map { meta, summary -> summary }
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.versions).match("versions") },
+                { assert process.out.png != null } // PNGs with same data but different meta-data. Not sure how to get around this, yet!
+            )
+        }
+
+    }
+
+    test("stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = file(params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/pfr/busco/generateplot/tests/main.nf.test.snap b/modules/pfr/busco/generateplot/tests/main.nf.test.snap
new file mode 100644
index 00000000..d9773ec6
--- /dev/null
+++ b/modules/pfr/busco/generateplot/tests/main.nf.test.snap
@@ -0,0 +1,37 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,726fa3440ea3a0b2e9d032d7e4d25e74"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T15:40:01.523993"
+    },
+    "stub": {
+        "content": [
+            {
+                "0": [
+                    "busco_figure.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "1": [
+                    "versions.yml:md5,726fa3440ea3a0b2e9d032d7e4d25e74"
+                ],
+                "png": [
+                    "busco_figure.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "versions": [
+                    "versions.yml:md5,726fa3440ea3a0b2e9d032d7e4d25e74"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-03T15:40:11.864276"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/busco/generateplot/tests/tags.yml b/modules/pfr/busco/generateplot/tests/tags.yml
new file mode 100644
index 00000000..b6548a64
--- /dev/null
+++ b/modules/pfr/busco/generateplot/tests/tags.yml
@@ -0,0 +1,2 @@
+busco/generateplot:
+  - "modules/nf-core/busco/generateplot/**"
diff --git a/modules/pfr/gffread/environment.yml b/modules/pfr/gffread/environment.yml
new file mode 100644
index 00000000..c6df58ad
--- /dev/null
+++ b/modules/pfr/gffread/environment.yml
@@ -0,0 +1,7 @@
+name: gffread
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::gffread=0.12.7
diff --git a/modules/pfr/gffread/main.nf b/modules/pfr/gffread/main.nf
new file mode 100644
index 00000000..da55cbab
--- /dev/null
+++ b/modules/pfr/gffread/main.nf
@@ -0,0 +1,60 @@
+process GFFREAD {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/gffread:0.12.7--hdcf5f25_4' :
+        'biocontainers/gffread:0.12.7--hdcf5f25_4' }"
+
+    input:
+    tuple val(meta), path(gff)
+    path fasta
+
+    output:
+    tuple val(meta), path("*.gtf")  , emit: gtf             , optional: true
+    tuple val(meta), path("*.gff3") , emit: gffread_gff     , optional: true
+    tuple val(meta), path("*.fasta"), emit: gffread_fasta   , optional: true
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args        = task.ext.args             ?: ''
+    def prefix      = task.ext.prefix           ?: "${meta.id}"
+    def extension   = args.contains("-T")       ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' )
+    def fasta_arg   = fasta                     ? "-g $fasta" : ''
+    def output_name = "${prefix}.${extension}"
+    def output      = extension == "fasta"      ? "$output_name" : "-o $output_name"
+    def args_sorted = args.replaceAll(/(.*)(-[wxy])(.*)/) { all, pre, param, post -> "$pre $post $param" }.trim()
+    // args_sorted  = Move '-w', '-x', and '-y' to the end of the args string as gffread expects the file name after these parameters
+    if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    gffread \\
+        $gff \\
+        $fasta_arg \\
+        $args_sorted \\
+        $output
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gffread: \$(gffread --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def args        = task.ext.args             ?: ''
+    def prefix      = task.ext.prefix           ?: "${meta.id}"
+    def extension   = args.contains("-T")       ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' )
+    def output_name = "${prefix}.${extension}"
+    if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    touch $output_name
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gffread: \$(gffread --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/gffread/meta.yml b/modules/pfr/gffread/meta.yml
new file mode 100644
index 00000000..c0602820
--- /dev/null
+++ b/modules/pfr/gffread/meta.yml
@@ -0,0 +1,55 @@
+name: gffread
+description: Validate, filter, convert and perform various other operations on GFF files
+keywords:
+  - gff
+  - conversion
+  - validation
+tools:
+  - gffread:
+      description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more.
+      homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread
+      documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread
+      tool_dev_url: https://github.com/gpertea/gffread
+      doi: 10.12688/f1000research.23297.1
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing meta data
+        e.g. [ id:'test' ]
+  - gff:
+      type: file
+      description: A reference file in either the GFF3, GFF2 or GTF format.
+      pattern: "*.{gff, gtf}"
+  - fasta:
+      type: file
+      description: A multi-fasta file with the genomic sequences
+      pattern: "*.{fasta,fa,faa,fas,fsa}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing meta data
+        e.g. [ id:'test' ]
+  - gtf:
+      type: file
+      description: GTF file resulting from the conversion of the GFF input file if '-T' argument is present
+      pattern: "*.{gtf}"
+  - gffread_gff:
+      type: file
+      description: GFF3 file resulting from the conversion of the GFF input file if '-T' argument is absent
+      pattern: "*.gff3"
+  - gffread_fasta:
+      type: file
+      description: Fasta file produced when either of '-w', '-x', '-y' parameters is present
+      pattern: "*.fasta"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@edmundmiller"
+maintainers:
+  - "@edmundmiller"
+  - "@gallvp"
diff --git a/modules/pfr/gffread/tests/main.nf.test b/modules/pfr/gffread/tests/main.nf.test
new file mode 100644
index 00000000..4cd13dcd
--- /dev/null
+++ b/modules/pfr/gffread/tests/main.nf.test
@@ -0,0 +1,223 @@
+nextflow_process {
+
+    name "Test Process GFFREAD"
+    script "../main.nf"
+    process "GFFREAD"
+
+    tag "gffread"
+    tag "modules_nfcore"
+    tag "modules"
+
+    test("sarscov2-gff3-gtf") {
+
+        config "./nextflow.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gffread_gff == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-gtf-stub") {
+
+        options '-stub'
+        config "./nextflow.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gffread_gff == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-gff3") {
+
+        config "./nextflow-gff3.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-gff3-stub") {
+
+        options '-stub'
+        config "./nextflow-gff3.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-fasta") {
+
+        config "./nextflow-fasta.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_gff == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-fasta-stub") {
+
+        options '-stub'
+        config "./nextflow-fasta.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_gff == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-fasta-fail-catch") {
+
+        options '-stub'
+        config "./nextflow-fasta.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'genome'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert ! process.success },
+                { assert process.stdout.toString().contains("Input and output names are the same") }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/pfr/gffread/tests/main.nf.test.snap b/modules/pfr/gffread/tests/main.nf.test.snap
new file mode 100644
index 00000000..15262320
--- /dev/null
+++ b/modules/pfr/gffread/tests/main.nf.test.snap
@@ -0,0 +1,272 @@
+{
+    "sarscov2-gff3-gtf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T10:48:56.496187"
+    },
+    "sarscov2-gff3-gff3": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91"
+                    ]
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T10:49:00.892782"
+    },
+    "sarscov2-gff3-gtf-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T11:11:26.975666"
+    },
+    "sarscov2-gff3-fasta-stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T11:11:44.34792"
+    },
+    "sarscov2-gff3-gff3-stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T11:11:35.221671"
+    },
+    "sarscov2-gff3-fasta": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a"
+                    ]
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T10:54:02.88143"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/gffread/tests/nextflow-fasta.config b/modules/pfr/gffread/tests/nextflow-fasta.config
new file mode 100644
index 00000000..ac6cb148
--- /dev/null
+++ b/modules/pfr/gffread/tests/nextflow-fasta.config
@@ -0,0 +1,5 @@
+process {
+    withName: GFFREAD {
+        ext.args = '-w -S'
+    }
+}
diff --git a/modules/pfr/gffread/tests/nextflow-gff3.config b/modules/pfr/gffread/tests/nextflow-gff3.config
new file mode 100644
index 00000000..afe0830e
--- /dev/null
+++ b/modules/pfr/gffread/tests/nextflow-gff3.config
@@ -0,0 +1,5 @@
+process {
+    withName: GFFREAD {
+        ext.args = ''
+    }
+}
diff --git a/modules/pfr/gffread/tests/nextflow.config b/modules/pfr/gffread/tests/nextflow.config
new file mode 100644
index 00000000..74b25094
--- /dev/null
+++ b/modules/pfr/gffread/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: GFFREAD {
+        ext.args = '-T'
+    }
+}
diff --git a/modules/pfr/gffread/tests/tags.yml b/modules/pfr/gffread/tests/tags.yml
new file mode 100644
index 00000000..05576065
--- /dev/null
+++ b/modules/pfr/gffread/tests/tags.yml
@@ -0,0 +1,2 @@
+gffread:
+  - modules/nf-core/gffread/**
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 28754b9f..44d71353 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -105,8 +105,8 @@
                 },
                 "busco_mode": {
                     "type": "string",
-                    "enum": ["geno", "tran", "prot", "genome", "transcriptome", "proteins"],
-                    "description": "BUSCO mode: 'geno' for genome, 'tran' for transcriptome, 'prot' for proteins"
+                    "enum": ["genome", "transcriptome", "proteins"],
+                    "description": "BUSCO mode: 'genome', 'transcriptome', 'proteins'"
                 },
                 "busco_lineage_datasets": {
                     "type": "string",
@@ -191,7 +191,7 @@
             "properties": {
                 "hic": {
                     "type": "string",
-                    "description": "HiC reads path provided as a SRA ID or as paired reads with pattern '*{1,2}.(fastq|fq).gz'",
+                    "description": "HiC reads path provided as a SRA ID or as paired reads with pattern '*{1,2}.fastq.gz'",
                     "pattern": "^SR\\w+$|^\\S+\\{1,2\\}\\.f(ast)?q\\.gz$"
                 },
                 "hic_skip_fastp": {
@@ -446,8 +446,7 @@
                     "type": "boolean",
                     "fa_icon": "far fa-check-circle",
                     "description": "Validation of parameters in lenient more.",
-                    "hidden": true,
-                    "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
+                    "hidden": true
                 }
             }
         }
diff --git a/subworkflows/local/fasta_busco_plot.nf b/subworkflows/local/fasta_busco_plot.nf
deleted file mode 100644
index f6cc5dc7..00000000
--- a/subworkflows/local/fasta_busco_plot.nf
+++ /dev/null
@@ -1,37 +0,0 @@
-include { BUSCO             } from '../../modules/local/busco'
-include { BUSCO_PLOT        } from '../../modules/local/busco_plot'
-
-workflow FASTA_BUSCO_PLOT {
-    take:
-    tuple_of_hap_file       // Channel
-    lineage                 // val
-    mode                    // val
-    download_path           // val; Use [] to use work directory. Useful on AWS
-
-    main:
-    ch_versions             = Channel.empty()
-
-    // MODULE: BUSCO
-    BUSCO(
-        tuple_of_hap_file,
-        lineage,
-        mode,
-        download_path
-    )
-
-    ch_busco_summaries      = BUSCO.out.summary
-                            | collect
-
-    ch_versions             = ch_versions.mix(BUSCO.out.versions.first())
-
-    // MODULE: BUSCO_PLOT
-    BUSCO_PLOT ( ch_busco_summaries )
-
-    ch_busco_plot           = BUSCO_PLOT.out.png
-    ch_versions             = ch_versions.mix(BUSCO_PLOT.out.versions.first())
-
-    emit:
-    summary                 = BUSCO.out.summary
-    plot                    = ch_busco_plot
-    versions                = ch_versions
-}
diff --git a/subworkflows/pfr/fasta_gxf_busco_plot/main.nf b/subworkflows/pfr/fasta_gxf_busco_plot/main.nf
new file mode 100644
index 00000000..555087d4
--- /dev/null
+++ b/subworkflows/pfr/fasta_gxf_busco_plot/main.nf
@@ -0,0 +1,168 @@
+include { BUSCO_BUSCO as BUSCO_ASSEMBLY         } from '../../../modules/pfr/busco/busco/main'
+include { BUSCO_GENERATEPLOT as PLOT_ASSEMBLY   } from '../../../modules/pfr/busco/generateplot/main'
+include { GFFREAD as EXTRACT_PROTEINS           } from '../../../modules/pfr/gffread/main'
+include { BUSCO_BUSCO as BUSCO_ANNOTATION       } from '../../../modules/pfr/busco/busco/main'
+include { BUSCO_GENERATEPLOT as PLOT_ANNOTATION } from '../../../modules/pfr/busco/generateplot/main'
+
+workflow FASTA_GXF_BUSCO_PLOT {
+
+    take:
+    ch_fasta                                    // channel: [ val(meta), fasta ]
+    ch_gxf                                      // channel: [ val(meta2), gxf ]; gxf ~ gff | gff3 | gtf
+                                                //
+                                                // Meta and meta2 should have same id
+
+    val_mode                                    // val(mode); BUSCO mode to apply to ch_fasta
+                                                // - genome, for genome assemblies (DNA)
+                                                // - transcriptome, for transcriptome assemblies (DNA)
+                                                // - proteins, for annotated gene sets (protein)
+                                                //
+                                                // If mode is genome, annotations from ch_gxf are evaluated with
+                                                // mode proteins, otherwise, evaluation of the annotations is skipped
+                                                //
+    val_lineages                                // [ val(lineage) ]
+    val_busco_lineages_path                     // val(path); Optional; Set to [] if not needed
+    val_busco_config                            // val(path); Optional; Set to [] if not needed
+
+    main:
+    ch_versions                                 = Channel.empty()
+    ch_db_path                                  = val_busco_lineages_path
+                                                ? Channel.of(file(val_busco_lineages_path, checkIfExists: true))
+                                                : Channel.of(null)
+    ch_config_path                              = val_busco_config
+                                                ? Channel.of(file(val_busco_config, checkIfExists: true))
+                                                : Channel.of(null)
+
+    // MODULE: BUSCO_BUSCO as BUSCO_ASSEMBLY
+    ch_busco_assembly_inputs                    = ch_fasta
+                                                | combine(
+                                                    Channel.of(val_mode)
+                                                )
+                                                | combine(
+                                                    Channel.fromList(val_lineages)
+                                                )
+                                                | map { meta, fasta, mode, lineage ->
+                                                    [
+                                                        meta + [ mode:mode, lineage:lineage ],
+                                                        fasta, mode, lineage
+                                                    ]
+                                                }
+                                                | combine(
+                                                    ch_db_path
+                                                )
+                                                | combine(
+                                                    ch_config_path
+                                                )
+
+    BUSCO_ASSEMBLY(
+        ch_busco_assembly_inputs.map { meta, fasta, mode, lineage, db, config -> [ meta, fasta ] },
+        ch_busco_assembly_inputs.map { meta, fasta, mode, lineage, db, config -> mode },
+        ch_busco_assembly_inputs.map { meta, fasta, mode, lineage, db, config -> lineage },
+        ch_busco_assembly_inputs.map { meta, fasta, mode, lineage, db, config -> db ?: [] },
+        ch_busco_assembly_inputs.map { meta, fasta, mode, lineage, db, config -> config ?: [] }
+    )
+
+    ch_assembly_batch_summary                   = BUSCO_ASSEMBLY.out.batch_summary
+    ch_assembly_short_summaries_txt             = BUSCO_ASSEMBLY.out.short_summaries_txt
+    ch_assembly_short_summaries_json            = BUSCO_ASSEMBLY.out.short_summaries_json
+    ch_versions                                 = ch_versions.mix(BUSCO_ASSEMBLY.out.versions.first())
+
+    // MODULE: BUSCO_GENERATEPLOT as PLOT_ASSEMBLY
+    ch_assembly_plot_inputs                     = ch_assembly_short_summaries_txt
+                                                | map { meta, txt ->
+                                                    def lineage_name = meta.lineage.split('_odb')[0]
+                                                    [
+                                                        "short_summary.specific.${meta.lineage}.${meta.id}_${lineage_name}.txt",
+                                                        txt.text
+                                                    ]
+                                                }
+                                                | collectFile
+                                                | collect
+
+    PLOT_ASSEMBLY( ch_assembly_plot_inputs )
+
+    ch_assembly_png                             = PLOT_ASSEMBLY.out.png
+    ch_versions                                 = ch_versions.mix(PLOT_ASSEMBLY.out.versions)
+
+    // MODULE: GFFREAD as EXTRACT_PROTEINS
+    ch_gffread_inputs                           = ! ( val_mode == 'geno' || val_mode == 'genome' )
+                                                ? Channel.empty()
+                                                : ch_fasta
+                                                | map { meta, fasta -> [ meta.id, meta, fasta ] }
+                                                | join(
+                                                    ch_gxf.map { meta2, gxf -> [ meta2.id, gxf ] }
+                                                    // Join with matching annotation
+                                                    // to allow one annotations per fasta
+                                                )
+                                                | map { id, meta, fasta, gxf -> [ meta, gxf, fasta ] }
+    EXTRACT_PROTEINS(
+        ch_gffread_inputs.map { meta, gxf, fasta -> [ meta, gxf ] },
+        ch_gffread_inputs.map { meta, gxf, fasta -> fasta }
+    )
+
+    ch_proteins                                 = EXTRACT_PROTEINS.out.gffread_fasta
+    ch_versions                                 = ch_versions.mix(EXTRACT_PROTEINS.out.versions.first())
+
+    // MODULE: BUSCO_BUSCO as BUSCO_ANNOTATION
+    ch_busco_annotation_inputs                  = ch_proteins
+                                                | combine(
+                                                    Channel.of('proteins')
+                                                )
+                                                | combine(
+                                                    Channel.fromList(val_lineages)
+                                                )
+                                                | map { meta, fasta, mode, lineage ->
+                                                    [
+                                                        meta + [ mode:mode, lineage:lineage ],
+                                                        fasta, mode, lineage
+                                                    ]
+                                                }
+                                                | combine(
+                                                    ch_db_path
+                                                )
+                                                | combine(
+                                                    ch_config_path
+                                                )
+
+    BUSCO_ANNOTATION(
+        ch_busco_annotation_inputs.map { meta, fasta, mode, lineage, db, config -> [ meta, fasta ] },
+        ch_busco_annotation_inputs.map { meta, fasta, mode, lineage, db, config -> mode },
+        ch_busco_annotation_inputs.map { meta, fasta, mode, lineage, db, config -> lineage },
+        ch_busco_annotation_inputs.map { meta, fasta, mode, lineage, db, config -> db ?: [] },
+        ch_busco_annotation_inputs.map { meta, fasta, mode, lineage, db, config -> config ?: [] }
+    )
+
+    ch_annotation_batch_summary                 = BUSCO_ANNOTATION.out.batch_summary
+    ch_annotation_short_summaries_txt           = BUSCO_ANNOTATION.out.short_summaries_txt
+    ch_annotation_short_summaries_json          = BUSCO_ANNOTATION.out.short_summaries_json
+    ch_versions                                 = ch_versions.mix(BUSCO_ANNOTATION.out.versions.first())
+
+    // MODULE: BUSCO_GENERATEPLOT as PLOT_ANNOTATION
+    ch_annotation_plot_inputs                   = ch_annotation_short_summaries_txt
+                                                | map { meta, txt ->
+                                                    def lineage_name = meta.lineage.split('_odb')[0]
+                                                    [
+                                                        "short_summary.specific.${meta.lineage}.${meta.id}_${lineage_name}.txt",
+                                                        txt.text
+                                                    ]
+                                                }
+                                                | collectFile
+                                                | collect
+
+    PLOT_ANNOTATION( ch_annotation_plot_inputs )
+
+    ch_annotation_png                           = PLOT_ANNOTATION.out.png
+    ch_versions                                 = ch_versions.mix(PLOT_ANNOTATION.out.versions)
+
+
+    emit:
+    assembly_batch_summary                      = ch_assembly_batch_summary             // channel: [ meta3, txt ]; meta3 ~ meta + [ val(mode), val(lineage) ]
+    assembly_short_summaries_txt                = ch_assembly_short_summaries_txt       // channel: [ meta3, txt ]
+    assembly_short_summaries_json               = ch_assembly_short_summaries_json      // channel: [ meta3, json ]
+    assembly_png                                = ch_assembly_png                       // channel: [ png ]
+    annotation_batch_summary                    = ch_annotation_batch_summary           // channel: [ meta3, txt ]
+    annotation_short_summaries_txt              = ch_annotation_short_summaries_txt     // channel: [ meta3, txt ]
+    annotation_short_summaries_json             = ch_annotation_short_summaries_json    // channel: [ meta3, json ]
+    annotation_png                              = ch_annotation_png                     // channel: [ png ]
+    versions                                    = ch_versions                           // channel: [ versions.yml ]
+}
diff --git a/subworkflows/pfr/fasta_gxf_busco_plot/meta.yml b/subworkflows/pfr/fasta_gxf_busco_plot/meta.yml
new file mode 100644
index 00000000..9226357e
--- /dev/null
+++ b/subworkflows/pfr/fasta_gxf_busco_plot/meta.yml
@@ -0,0 +1,106 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "fasta_gxf_busco_plot"
+description: |
+  Runs BUSCO for input assemblies and their annotations in GFF/GFF3/GTF format, and creates summary plots using `BUSCO/generate_plot.py` script
+keywords:
+  - genome
+  - annotation
+  - busco
+  - plot
+components:
+  - busco/busco
+  - busco/generateplot
+  - gffread
+input:
+  - ch_fasta:
+      type: file
+      description: |
+        Channel containing FASTA files
+        Structure:[ val(meta), fasta ]
+      pattern: "*.{fa,faa,fsa,fas,fasta}(.gz)?"
+  - ch_gxf:
+      type: file
+      description: |
+        Channel containing GFF/GFF3/GTF files
+        Structure:[ val(meta2), gxf ]
+      pattern: "*.{gff,gff3,gtf}"
+  - val_mode:
+      type: string
+      description: |
+        String containing BUSCO mode to apply to ch_fasta files
+        Structure:val(mode)
+  - val_lineages:
+      type: array
+      description: |
+        Array of strings representing BUSCO lineage datasets
+        Structure:[ val(lineage) ]
+  - val_busco_lineages_path:
+      type: path
+      description: |
+        Path where BUSCO lineages are located or downloaded if not already there. If this input is `[]`,
+        BUSCO will download the datasets in the task work directory
+        Structure:val(busco_lineages_path)
+  - val_busco_config:
+      type: path
+      description: |
+        Path to BUSCO config. It is optional and can be set to `[]`
+        Structure:val(busco_config)
+output:
+  - assembly_batch_summary:
+      type: file
+      description: |
+        Channel containing BUSCO batch summaries corresponding to fasta files
+        Structure: [ val(meta), txt ]
+      pattern: "*.txt"
+  - assembly_short_summaries_txt:
+      type: file
+      description: |
+        Channel containing BUSCO short summaries corresponding to fasta files
+        Structure: [ val(meta), txt ]
+      pattern: "*.txt"
+  - assembly_short_summaries_json:
+      type: file
+      description: |
+        Channel containing BUSCO short summaries corresponding to fasta files
+        Structure: [ val(meta), json ]
+      pattern: "*.json"
+  - assembly_png:
+      type: file
+      description: |
+        Channel containing summary plot for assemblies
+        Structure: png
+      pattern: "*.png"
+  - annotation_batch_summary:
+      type: file
+      description: |
+        Channel containing BUSCO batch summaries corresponding to annotation files
+        Structure: [ val(meta), txt ]
+      pattern: "*.txt"
+  - annotation_short_summaries_txt:
+      type: file
+      description: |
+        Channel containing BUSCO short summaries corresponding to annotation files
+        Structure: [ val(meta), txt ]
+      pattern: "*.txt"
+  - annotation_short_summaries_json:
+      type: file
+      description: |
+        Channel containing BUSCO short summaries corresponding to annotation files
+        Structure: [ val(meta), json ]
+      pattern: "*.json"
+  - annotation_png:
+      type: file
+      description: |
+        Channel containing summary plot for annotations
+        Structure: png
+      pattern: "*.png"
+  - versions:
+      type: file
+      description: |
+        File containing software versions
+        Structure: [ path(versions.yml) ]
+      pattern: "versions.yml"
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test b/subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test
new file mode 100644
index 00000000..8c8ac7b0
--- /dev/null
+++ b/subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test
@@ -0,0 +1,104 @@
+nextflow_workflow {
+
+    name "Test Subworkflow FASTA_GXF_BUSCO_PLOT"
+    script "../main.nf"
+    workflow "FASTA_GXF_BUSCO_PLOT"
+    config './nextflow.config'
+
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/fasta_gxf_busco_plot"
+    tag "busco"
+    tag "busco/busco"
+    tag "busco/generateplot"
+    tag "gffread"
+
+    test("candidatus_portiera_aleyrodidarum - bacteroides_fragilis - genome") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of(
+                    [
+                        [ id:'test' ], // meta map
+                        file( params.test_data['candidatus_portiera_aleyrodidarum']['genome']['genome_fasta'], checkIfExists: true)
+                    ],
+                    [
+                        [ id:'test2' ], // meta map
+                        file( params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                    ]
+                )
+                input[1] = Channel.of(
+                    [
+                        [ id:'test' ], // meta map
+                        file( params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test1_gff'], checkIfExists: true)
+                    ]
+                )
+                input[2] = 'genome'
+                input[3] = [ 'bacteria_odb10', 'archaea_odb10' ]
+                input[4] = []
+                input[5] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    workflow.out.assembly_batch_summary,
+                    workflow.out.annotation_batch_summary,
+                    workflow.out.versions,
+                    ).match()
+                },
+
+                { assert workflow.out.assembly_png != null },
+                { assert workflow.out.annotation_png != null },
+
+                { assert workflow.out.assembly_short_summaries_json != null },
+                { assert workflow.out.assembly_short_summaries_txt != null },
+                { assert workflow.out.annotation_short_summaries_json != null },
+                { assert workflow.out.annotation_short_summaries_txt != null }
+            )
+        }
+    }
+
+    test("candidatus_portiera_aleyrodidarum - bacteroides_fragilis - genome - stub") {
+
+        options '-stub'
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of(
+                    [
+                        [ id:'test' ], // meta map
+                        file( params.test_data['candidatus_portiera_aleyrodidarum']['genome']['genome_fasta'], checkIfExists: true)
+                    ],
+                    [
+                        [ id:'test2' ], // meta map
+                        file( params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                    ]
+                )
+                input[1] = Channel.of(
+                    [
+                        [ id:'test' ], // meta map
+                        file( params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test1_gff'], checkIfExists: true)
+                    ]
+                )
+                input[2] = 'genome'
+                input[3] = [ 'bacteria_odb10', 'archaea_odb10' ]
+                input[4] = []
+                input[5] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match()}
+            )
+        }
+    }
+}
diff --git a/subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test.snap b/subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test.snap
new file mode 100644
index 00000000..118ad7ed
--- /dev/null
+++ b/subworkflows/pfr/fasta_gxf_busco_plot/tests/main.nf.test.snap
@@ -0,0 +1,231 @@
+{
+    "candidatus_portiera_aleyrodidarum - bacteroides_fragilis - genome - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "mode": "genome",
+                            "lineage": "archaea_odb10"
+                        },
+                        "test-archaea_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test",
+                            "mode": "genome",
+                            "lineage": "bacteria_odb10"
+                        },
+                        "test-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test2",
+                            "mode": "genome",
+                            "lineage": "archaea_odb10"
+                        },
+                        "test2-archaea_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test2",
+                            "mode": "genome",
+                            "lineage": "bacteria_odb10"
+                        },
+                        "test2-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "mode": "proteins",
+                            "lineage": "archaea_odb10"
+                        },
+                        "test-archaea_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test",
+                            "mode": "proteins",
+                            "lineage": "bacteria_odb10"
+                        },
+                        "test-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    
+                ],
+                "8": [
+                    "versions.yml:md5,36b11c442943567e471af0abd474a10b",
+                    "versions.yml:md5,9435355f913e283f60b4fb7ef77dd52a",
+                    "versions.yml:md5,e9d65e2f2f13175e99c5b7f4ae1013b9"
+                ],
+                "annotation_batch_summary": [
+                    [
+                        {
+                            "id": "test",
+                            "mode": "proteins",
+                            "lineage": "archaea_odb10"
+                        },
+                        "test-archaea_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test",
+                            "mode": "proteins",
+                            "lineage": "bacteria_odb10"
+                        },
+                        "test-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "annotation_png": [
+                    
+                ],
+                "annotation_short_summaries_json": [
+                    
+                ],
+                "annotation_short_summaries_txt": [
+                    
+                ],
+                "assembly_batch_summary": [
+                    [
+                        {
+                            "id": "test",
+                            "mode": "genome",
+                            "lineage": "archaea_odb10"
+                        },
+                        "test-archaea_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test",
+                            "mode": "genome",
+                            "lineage": "bacteria_odb10"
+                        },
+                        "test-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test2",
+                            "mode": "genome",
+                            "lineage": "archaea_odb10"
+                        },
+                        "test2-archaea_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ],
+                    [
+                        {
+                            "id": "test2",
+                            "mode": "genome",
+                            "lineage": "bacteria_odb10"
+                        },
+                        "test2-bacteria_odb10-busco.batch_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "assembly_png": [
+                    
+                ],
+                "assembly_short_summaries_json": [
+                    
+                ],
+                "assembly_short_summaries_txt": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,36b11c442943567e471af0abd474a10b",
+                    "versions.yml:md5,9435355f913e283f60b4fb7ef77dd52a",
+                    "versions.yml:md5,e9d65e2f2f13175e99c5b7f4ae1013b9"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-13T16:39:45.021811"
+    },
+    "candidatus_portiera_aleyrodidarum - bacteroides_fragilis - genome": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "mode": "genome",
+                        "lineage": "archaea_odb10"
+                    },
+                    "test-archaea_odb10-busco.batch_summary.txt:md5,1397d74518a776ad75b16a843bc5b6c1"
+                ],
+                [
+                    {
+                        "id": "test",
+                        "mode": "genome",
+                        "lineage": "bacteria_odb10"
+                    },
+                    "test-bacteria_odb10-busco.batch_summary.txt:md5,a1186bc25448ac1949bf7790810f7161"
+                ],
+                [
+                    {
+                        "id": "test2",
+                        "mode": "genome",
+                        "lineage": "archaea_odb10"
+                    },
+                    "test2-archaea_odb10-busco.batch_summary.txt:md5,946582b353a8dba7d6452a71856eca06"
+                ],
+                [
+                    {
+                        "id": "test2",
+                        "mode": "genome",
+                        "lineage": "bacteria_odb10"
+                    },
+                    "test2-bacteria_odb10-busco.batch_summary.txt:md5,21b3fb771cf36be917cc451540d999be"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "mode": "proteins",
+                        "lineage": "archaea_odb10"
+                    },
+                    "test-archaea_odb10-busco.batch_summary.txt:md5,95172bd5b1a30e632fc79084ea0ca585"
+                ],
+                [
+                    {
+                        "id": "test",
+                        "mode": "proteins",
+                        "lineage": "bacteria_odb10"
+                    },
+                    "test-bacteria_odb10-busco.batch_summary.txt:md5,995127c0caecb36205dbf21aa2f9f8a8"
+                ]
+            ],
+            [
+                "versions.yml:md5,05d8022e3afb0d5642ed17147b991730",
+                "versions.yml:md5,36b11c442943567e471af0abd474a10b",
+                "versions.yml:md5,53987b35fc275297efdaf525937fdca3",
+                "versions.yml:md5,9435355f913e283f60b4fb7ef77dd52a",
+                "versions.yml:md5,e9d65e2f2f13175e99c5b7f4ae1013b9"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-13T16:39:04.376704"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/pfr/fasta_gxf_busco_plot/tests/nextflow.config b/subworkflows/pfr/fasta_gxf_busco_plot/tests/nextflow.config
new file mode 100644
index 00000000..ddbc644d
--- /dev/null
+++ b/subworkflows/pfr/fasta_gxf_busco_plot/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: EXTRACT_PROTEINS {
+        ext.args = '-y'
+    }
+}
diff --git a/subworkflows/pfr/fasta_gxf_busco_plot/tests/tags.yml b/subworkflows/pfr/fasta_gxf_busco_plot/tests/tags.yml
new file mode 100644
index 00000000..b8786a59
--- /dev/null
+++ b/subworkflows/pfr/fasta_gxf_busco_plot/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/fasta_gxf_busco_plot:
+  - subworkflows/pfr/fasta_gxf_busco_plot/**
diff --git a/tests/stub/stub.config b/tests/stub/stub.config
index 0eb35b5c..7e300f59 100644
--- a/tests/stub/stub.config
+++ b/tests/stub/stub.config
@@ -12,7 +12,7 @@ params {
     ncbi_fcs_gx_db_path         = 'tests/stub/gxdb/test'
 
     busco_skip                  = false
-    busco_mode                  = 'geno'
+    busco_mode                  = 'genome'
     busco_lineage_datasets      = 'fungi_odb10 hypocreales_odb10'
 
     tidk_skip                   = false
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index eb68d088..2d8dd5b0 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -15,7 +15,7 @@ include { GFF3_GT_GFF3_GFF3VALIDATOR_STAT   } from '../subworkflows/pfr/gff3_gt_
 include { NCBI_FCS_ADAPTOR                  } from '../modules/local/ncbi_fcs_adaptor'
 include { NCBI_FCS_GX                       } from '../subworkflows/local/ncbi_fcs_gx'
 include { ASSEMBLATHON_STATS                } from '../modules/local/assemblathon_stats'
-include { FASTA_BUSCO_PLOT                  } from '../subworkflows/local/fasta_busco_plot'
+include { FASTA_GXF_BUSCO_PLOT              } from '../subworkflows/pfr/fasta_gxf_busco_plot/main'
 include { FASTA_LTRRETRIEVER_LAI            } from '../subworkflows/pfr/fasta_ltrretriever_lai/main'
 include { FASTA_KRAKEN2                     } from '../subworkflows/local/fasta_kraken2'
 include { FQ2HIC                            } from '../subworkflows/local/fq2hic'
@@ -125,7 +125,7 @@ workflow ASSEMBLYQC {
                                             | map { meta, error_log ->
                                                 log.warn("FASTA validation failed for ${meta.id}\n${error_log.text}")
 
-                                                [ meta, error_log ]
+                                                error_log
                                             }
 
     ch_versions                             = ch_versions.mix(FASTAVALIDATOR.out.versions.first())
@@ -136,11 +136,12 @@ workflow ASSEMBLYQC {
         ch_valid_target_assembly
     )
 
+    ch_valid_gff3                           = GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.valid_gff3
     ch_invalid_gff3_log                     = GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.log_for_invalid_gff3
                                             | map { meta, error_log ->
                                                 log.warn("GFF3 validation failed for ${meta.id}\n${error_log.text}")
 
-                                                [ meta, error_log ]
+                                                error_log
                                             }
 
     ch_gt_stats                             = GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.gff3_stats
@@ -267,28 +268,53 @@ workflow ASSEMBLYQC {
     ch_assemblathon_stats                   = ASSEMBLATHON_STATS.out.stats
     ch_versions                             = ch_versions.mix(ASSEMBLATHON_STATS.out.versions.first())
 
-    // SUBWORKFLOW: FASTA_BUSCO_PLOT
-    ch_busco_inputs                         = params.busco_skip
+    // SUBWORKFLOW: FASTA_GXF_BUSCO_PLOT
+    ch_busco_input_assembly                 = params.busco_skip
                                             ? Channel.empty()
                                             : ch_clean_assembly
-                                            | combine(
-                                                Channel.of(params.busco_lineage_datasets)
-                                                | map { it.split(' ') }
-                                                | flatten
-                                            )
-                                            | map { tag, fa, lineage ->
-                                                [ tag, file(fa, checkIfExists: true), lineage ]
-                                            }
-    FASTA_BUSCO_PLOT(
-        ch_busco_inputs.map { tag, fa, lineage -> [ tag, fa ] },
-        ch_busco_inputs.map { tag, fa, lineage -> lineage },
-        params.busco_mode ?: [],
-        params.busco_download_path ?: []
+                                            | map { tag, fasta -> [ [ id: tag ], fasta ] }
+
+    FASTA_GXF_BUSCO_PLOT(
+        ch_busco_input_assembly,
+        ch_valid_gff3,
+        params.busco_mode,
+        params.busco_lineage_datasets?.tokenize(' '),
+        params.busco_download_path,
+        [] // val_busco_config
     )
 
-    ch_busco_summary                        = FASTA_BUSCO_PLOT.out.summary
-    ch_busco_plot                           = FASTA_BUSCO_PLOT.out.plot
-    ch_versions                             = ch_versions.mix(FASTA_BUSCO_PLOT.out.versions)
+    ch_busco_summary                        = FASTA_GXF_BUSCO_PLOT.out.assembly_short_summaries_txt
+                                            | map { meta, txt ->
+                                                def lineage_name = meta.lineage.split('_odb')[0]
+                                                [
+                                                    "short_summary.specific.${meta.lineage}.${meta.id}_${lineage_name}.txt",
+                                                    txt.text
+                                                ]
+                                            }
+                                            | collectFile
+    ch_busco_plot                           = FASTA_GXF_BUSCO_PLOT.out.assembly_png
+
+    ch_busco_outputs                        = ch_busco_summary
+                                            | mix(ch_busco_plot)
+                                            | collect
+
+    ch_busco_gff_summary                    = FASTA_GXF_BUSCO_PLOT.out.annotation_short_summaries_txt
+                                            | map { meta, txt ->
+                                                def lineage_name = meta.lineage.split('_odb')[0]
+                                                [
+                                                    "short_summary.specific.${meta.lineage}.${meta.id}_${lineage_name}.txt",
+                                                    txt.text
+                                                ]
+                                            }
+                                            | collectFile
+
+    ch_busco_gff_plot                       = FASTA_GXF_BUSCO_PLOT.out.annotation_png
+
+    ch_busco_gff_outputs                    = ch_busco_gff_summary
+                                            | mix(ch_busco_gff_plot)
+                                            | collect
+
+    ch_versions                             = ch_versions.mix(FASTA_GXF_BUSCO_PLOT.out.versions)
 
     // SUBWORKFLOW: FASTA_EXPLORE_SEARCH_PLOT_TIDK
     ch_tidk_inputs                          = params.tidk_skip
@@ -500,13 +526,14 @@ workflow ASSEMBLYQC {
 
     // MODULE: CREATEREPORT
     CREATEREPORT(
-        ch_invalid_assembly_log             .map { meta, file -> file }.collect().ifEmpty([]),
-        ch_invalid_gff3_log                 .map { meta, file -> file }.collect().ifEmpty([]),
+        ch_invalid_assembly_log             .collect().ifEmpty([]),
+        ch_invalid_gff3_log                 .collect().ifEmpty([]),
         ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
         ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
         ch_assemblathon_stats               .collect().ifEmpty([]),
         ch_gt_stats                         .collect().ifEmpty([]),
-        ch_busco_summary                    .mix(ch_busco_plot).collect().ifEmpty([]),
+        ch_busco_outputs                    .collect().ifEmpty([]),
+        ch_busco_gff_outputs                .collect().ifEmpty([]),
         ch_tidk_outputs                     .collect().ifEmpty([]),
         ch_lai_outputs                      .collect().ifEmpty([]),
         ch_kraken2_plot                     .collect().ifEmpty([]),

From 32efaaf21b619f735872a7bdc47dcd534c568288 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 17 May 2024 13:18:53 +1200
Subject: [PATCH 41/76] Replaced local NCBI_FCS_ADAPTOR with nf-core module

---
 .github/workflows/ci.yml                      | 23 +++--
 CHANGELOG.md                                  |  1 +
 README.md                                     |  1 +
 .../parsers/ncbi_fcs_adaptor_parser.py        |  4 +-
 .../ncbi_fcs_adaptor/ncbi_fcs_adaptor.html    |  2 +-
 conf/modules.config                           |  6 +-
 conf/test.config                              |  2 +-
 modules.json                                  |  5 ++
 modules/local/generatekaryotype.nf            | 14 +++
 modules/local/ncbi_fcs_adaptor.nf             | 58 -------------
 .../nf-core/fcs/fcsadaptor/environment.yml    |  7 ++
 modules/nf-core/fcs/fcsadaptor/main.nf        | 87 +++++++++++++++++++
 modules/nf-core/fcs/fcsadaptor/meta.yml       | 64 ++++++++++++++
 .../nf-core/fcs/fcsadaptor/tests/main.nf.test | 73 ++++++++++++++++
 .../fcs/fcsadaptor/tests/main.nf.test.snap    | 57 ++++++++++++
 modules/nf-core/fcs/fcsadaptor/tests/tags.yml |  2 +
 tests/minimal/params.json                     |  8 ++
 tests/stub/params.json                        | 27 ++++++
 tests/stub/stub.config                        | 37 --------
 workflows/assemblyqc.nf                       | 35 ++++----
 20 files changed, 385 insertions(+), 128 deletions(-)
 delete mode 100644 modules/local/ncbi_fcs_adaptor.nf
 create mode 100644 modules/nf-core/fcs/fcsadaptor/environment.yml
 create mode 100644 modules/nf-core/fcs/fcsadaptor/main.nf
 create mode 100644 modules/nf-core/fcs/fcsadaptor/meta.yml
 create mode 100644 modules/nf-core/fcs/fcsadaptor/tests/main.nf.test
 create mode 100644 modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/fcs/fcsadaptor/tests/tags.yml
 create mode 100644 tests/minimal/params.json
 create mode 100644 tests/stub/params.json
 delete mode 100644 tests/stub/stub.config

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 803c1acb..89f347df 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -25,10 +25,14 @@ jobs:
       matrix:
         NXF_VER:
           - "23.04.0"
-        RUN_CONFIG:
-          - "-profile test,docker"
-          - "-profile docker -c ./tests/stub/stub.config -stub"
-          - "-profile docker -params-file ./tests/invalid/params.json"
+        TEST_PARAMS:
+          - minimal
+          - invalid
+          - stub
+        include:
+          - OPTION_STUB: ""
+          - OPTION_STUB: "-stub"
+            TEST_PARAMS: stub
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
@@ -41,9 +45,12 @@ jobs:
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - name: Disk space cleanup
-        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
-
       - name: Run pipeline with test data
         run: |
-          nextflow run ${GITHUB_WORKSPACE} --outdir ./results ${{ matrix.RUN_CONFIG }}
+          nextflow run \
+            ${GITHUB_WORKSPACE} \
+            --outdir ./results \
+            -profile docker \
+            -params-file \
+            ./tests/${{ matrix.TEST_PARAMS }}/params.json \
+            ${{ matrix.OPTION_STUB }}
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 29047314..0488e6be 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,6 +22,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 13. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
 14. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
 15. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
+16. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module
 
 ### `Fixed`
 
diff --git a/README.md b/README.md
index febc6ddc..f30501ce 100644
--- a/README.md
+++ b/README.md
@@ -137,6 +137,7 @@ The pipeline uses nf-core modules contributed by following authors.
 <a href="https://github.com/felixkrueger"><img src="https://github.com/felixkrueger.png" width="50" height="50"></a>
 <a href="https://github.com/erikrikarddaniel"><img src="https://github.com/erikrikarddaniel.png" width="50" height="50"></a>
 <a href="https://github.com/edmundmiller"><img src="https://github.com/edmundmiller.png" width="50" height="50"></a>
+<a href="https://github.com/d4straub"><img src="https://github.com/d4straub.png" width="50" height="50"></a>
 <a href="https://github.com/charles-plessy"><img src="https://github.com/charles-plessy.png" width="50" height="50"></a>
 
 ## Contributions and Support
diff --git a/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py b/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py
index f896577f..8451d767 100644
--- a/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py
+++ b/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py
@@ -14,7 +14,7 @@ def parse_ncbi_fcs_adaptor_folder(folder_name="ncbi_fcs_adaptor_reports"):
     if not os.path.exists(reports_folder_path):
         return {}
 
-    list_of_report_files = reports_folder_path.glob("*.tsv")
+    list_of_report_files = reports_folder_path.glob("*.txt")
 
     data = {"NCBI_FCS_ADAPTOR": []}
 
@@ -22,7 +22,7 @@ def parse_ncbi_fcs_adaptor_folder(folder_name="ncbi_fcs_adaptor_reports"):
         report_table = pd.read_csv(report_path, sep="\t")
 
         file_tokens = re.findall(
-            r"([\w]+)_fcs_adaptor_report.tsv",
+            r"([\w]+)\.fcs_adaptor_report.txt",
             os.path.basename(str(report_path)),
         )[0]
 
diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html
index e62678c9..4c95333f 100644
--- a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html
+++ b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html
@@ -5,7 +5,7 @@
         <p class="section-para">
             <a href="https://github.com/ncbi/fcs" target="_blank">https://github.com/ncbi/fcs</a>
         </p>
-        <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['NCBI_FCS_ADAPTOR']['av_screen_x'] }}</b></p>
+        <p class="section-para"><b>Version: {{ all_stats_dicts['VERSIONS']['FCS_FCSADAPTOR']['FCS-adaptor'] }}</b></p>
     </div>
     {% include 'ncbi_fcs_adaptor/dropdown.html' %} {% include 'ncbi_fcs_adaptor/report_contents.html' %}
 </div>
diff --git a/conf/modules.config b/conf/modules.config
index 028955ff..e63a0cdb 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -42,11 +42,13 @@ process {
         ]
     }
 
-    withName: NCBI_FCS_ADAPTOR {
+    withName: FCS_FCSADAPTOR {
+        ext.args = params.ncbi_fcs_adaptor_empire ? "--${params.ncbi_fcs_adaptor_empire}" : '--prok'
+
         publishDir = [
             path: { "${params.outdir}/ncbi_fcs_adaptor" },
             mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
+            pattern: '*.fcs_adaptor_report.txt'
         ]
     }
 
diff --git a/conf/test.config b/conf/test.config
index 3ba5bef2..b48e728f 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -14,7 +14,7 @@ params {
     config_profile_name         = 'Test profile'
     config_profile_description  = 'Minimal test dataset to check pipeline function'
 
-    input                       = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv'
+    input                       = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv'
 
     // Limit resources so that this can run on GitHub Actions
     max_cpus                    = 2
diff --git a/modules.json b/modules.json
index 0fff3420..d2896927 100644
--- a/modules.json
+++ b/modules.json
@@ -145,6 +145,11 @@
                         "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
                         "installed_by": ["fastq_trim_fastp_fastqc"]
                     },
+                    "fcs/fcsadaptor": {
+                        "branch": "master",
+                        "git_sha": "28adf00792d7dfd1b9ecbde9d7a8f22ec78ffcbb",
+                        "installed_by": ["modules"]
+                    },
                     "gunzip": {
                         "branch": "master",
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
diff --git a/modules/local/generatekaryotype.nf b/modules/local/generatekaryotype.nf
index b5ed48fa..e3771fe6 100644
--- a/modules/local/generatekaryotype.nf
+++ b/modules/local/generatekaryotype.nf
@@ -70,4 +70,18 @@ process GENERATEKARYOTYPE {
 
     rm "\$tmp_file"
     """
+
+    stub:
+    """
+    touch "${target_on_ref}.${seq_tag}.karyotype"
+    touch karyotype_ref.tsv
+    touch karyotype_target.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        awk: \$(awk -W version | sed -n 's/mawk //p')
+        grep: \$(grep --version | sed -n '/grep (GNU grep) /s/grep //p')
+        sed: \$(sed --version | sed -n 's/^sed //p')
+    END_VERSIONS
+    """
 }
diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf
deleted file mode 100644
index 3db1b3ba..00000000
--- a/modules/local/ncbi_fcs_adaptor.nf
+++ /dev/null
@@ -1,58 +0,0 @@
-process NCBI_FCS_ADAPTOR {
-    tag "${asm_tag}"
-    label 'process_single'
-
-    // Warning: manually update version in script and stub
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.5.0/fcs-adaptor.sif':
-        'docker.io/ncbi/fcs-adaptor:0.5.0' }"
-
-    input:
-    tuple val(asm_tag), path(fasta_file)
-    val empire
-
-    output:
-    tuple val(asm_tag), path("${asm_tag}_fcs_adaptor_report.tsv") , emit: report
-    path "versions.yml"                                           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def VERSION = 0.5
-    """
-    mkdir "${asm_tag}_outputdir"
-
-    av_screen_x \\
-        -o "${asm_tag}_outputdir" \\
-        --${empire} \\
-        "${fasta_file}"
-
-    mv "${asm_tag}_outputdir/fcs_adaptor_report.txt" \\
-        "./${asm_tag}_fcs_adaptor_report.tsv"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        av_screen_x: $VERSION
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def VERSION = 0.5
-    """
-    touch "${asm_tag}_fcs_adaptor_report.tsv"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        av_screen_x: $VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/fcs/fcsadaptor/environment.yml b/modules/nf-core/fcs/fcsadaptor/environment.yml
new file mode 100644
index 00000000..b11cd415
--- /dev/null
+++ b/modules/nf-core/fcs/fcsadaptor/environment.yml
@@ -0,0 +1,7 @@
+name: fcs_fcsadaptor
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::false_flag # False flag to pass nf-core/lint
diff --git a/modules/nf-core/fcs/fcsadaptor/main.nf b/modules/nf-core/fcs/fcsadaptor/main.nf
new file mode 100644
index 00000000..5772afec
--- /dev/null
+++ b/modules/nf-core/fcs/fcsadaptor/main.nf
@@ -0,0 +1,87 @@
+process FCS_FCSADAPTOR {
+    tag "$meta.id"
+    label 'process_low'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.5.0/fcs-adaptor.sif':
+        'docker.io/ncbi/fcs-adaptor:0.5.0' }"
+
+    input:
+    tuple val(meta), path(assembly)
+
+    output:
+    tuple val(meta), path("*.cleaned_sequences.fa.gz"), emit: cleaned_assembly, optional: true
+    tuple val(meta), path("*.fcs_adaptor_report.txt") , emit: adaptor_report
+    tuple val(meta), path("*.fcs_adaptor.log")        , emit: log
+    tuple val(meta), path("*.pipeline_args.yaml")     , emit: pipeline_args
+    tuple val(meta), path("*.skipped_trims.jsonl")    , emit: skipped_trims
+    path "versions.yml"                               , emit: versions
+
+    // Downstream handling of optional cleaned_assembly
+    //
+    // ch_cleaned_assembly  = FCS_FCSADAPTOR ( ch_input_assembly ).cleaned_assembly
+
+    // ch_input_assembly
+    // | join (ch_cleaned_assembly, by:0, remainder:true )
+    // | map { meta, input, cleaned ->
+    //     [ meta, cleaned ?: input ]
+    // }
+    // | set { ch_downstream_channel }
+
+    // FCS_FCSGX ( ch_downstream_channel )
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "FCS_FCSADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: '--prok' // --prok || --euk
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def FCSADAPTOR_VERSION = '0.5.0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    av_screen_x \\
+        -o output/ \\
+        $args \\
+        $assembly
+
+    # compress and/or rename files with prefix
+    num_contamination_lines=\$(cat "output/fcs_adaptor_report.txt" | wc -l)
+    if [[ \$num_contamination_lines -gt 1 ]]; then
+        find output/cleaned_sequences/  -type f ! -name "*.gz" -exec gzip {} \\;
+        cp output/cleaned_sequences/*     "${prefix}.cleaned_sequences.fa.gz"
+    fi
+    cp "output/fcs_adaptor_report.txt"    "${prefix}.fcs_adaptor_report.txt"
+    cp "output/fcs_adaptor.log"           "${prefix}.fcs_adaptor.log"
+    cp "output/pipeline_args.yaml"        "${prefix}.pipeline_args.yaml"
+    cp "output/skipped_trims.jsonl"       "${prefix}.skipped_trims.jsonl"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        FCS-adaptor: $FCSADAPTOR_VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "FCS_FCSADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def FCSADAPTOR_VERSION = '0.5.0'
+
+    """
+    touch ${prefix}.fcs_adaptor_report.txt
+    touch ${prefix}.fcs_adaptor.log
+    touch ${prefix}.pipeline_args.yaml
+    touch ${prefix}.skipped_trims.jsonl
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        FCS-adaptor: $FCSADAPTOR_VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/fcs/fcsadaptor/meta.yml b/modules/nf-core/fcs/fcsadaptor/meta.yml
new file mode 100644
index 00000000..54fca1bb
--- /dev/null
+++ b/modules/nf-core/fcs/fcsadaptor/meta.yml
@@ -0,0 +1,64 @@
+name: "fcs_fcsadaptor"
+description: Run NCBI's FCS adaptor on assembled genomes
+keywords:
+  - assembly
+  - genomics
+  - quality control
+  - contamination
+  - NCBI
+tools:
+  - "fcs":
+      description: |
+        The Foreign Contamination Screening (FCS) tool rapidly detects contaminants from foreign
+        organisms in genome assemblies to prepare your data for submission. Therefore, the
+        submission process to NCBI is faster and fewer contaminated genomes are submitted.
+        This reduces errors in analyses and conclusions, not just for the original data submitter
+        but for all subsequent users of the assembly.
+      homepage: "https://www.ncbi.nlm.nih.gov/data-hub/cgr/data-quality-tools/"
+      documentation: "https://github.com/ncbi/fcs/wiki/FCS-adaptor"
+      tool_dev_url: "https://github.com/ncbi/fcs"
+      licence: ["United States Government Work"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - assembly:
+      type: file
+      description: assembly fasta file
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - cleaned_assembly:
+      type: file
+      description: Cleaned assembly in fasta format
+      pattern: "*.{cleaned_sequences.fa.gz}"
+  - adaptor_report:
+      type: file
+      description: Report of identified adaptors
+      pattern: "*.{fcs_adaptor_report.txt}"
+  - log:
+      type: file
+      description: Log file
+      pattern: "*.{fcs_adaptor.log}"
+  - pipeline_args:
+      type: file
+      description: Run arguments
+      pattern: "*.{pipeline_args.yaml}"
+  - skipped_trims:
+      type: file
+      description: Skipped trim information
+      pattern: "*.{skipped_trims.jsonl}"
+authors:
+  - "@d4straub"
+maintainers:
+  - "@d4straub"
+  - "@gallvp"
diff --git a/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test
new file mode 100644
index 00000000..9d647490
--- /dev/null
+++ b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test
@@ -0,0 +1,73 @@
+nextflow_process {
+
+    name "Test Process FCS_FCSADAPTOR"
+    script "../main.nf"
+    process "FCS_FCSADAPTOR"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "fcs"
+    tag "fcs/fcsadaptor"
+
+    test("bacteroides_fragilis-genome_fna_gz") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id: 'test' ],
+                    file(params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.cleaned_assembly == [] },
+                { assert process.out.skipped_trims != null }, // md5sum for empty file not allowed in nf-core/tools 2.14.0
+                { assert snapshot(
+                    process.out.adaptor_report,
+                    process.out.pipeline_args,
+                    process.out.versions,
+                    ).match()
+                },
+                { assert path(process.out.log[0][1]).text.contains('[step all_cleaned_fasta] completed success') }
+            )
+        }
+
+    }
+    test("bacteroides_fragilis-genome_fna_gz-stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id: 'test' ],
+                    file(params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.cleaned_assembly == [] },
+                { assert snapshot(
+                    [
+                        process.out.adaptor_report[0][1],
+                        process.out.log[0][1],
+                        process.out.pipeline_args[0][1],
+                        process.out.skipped_trims[0][1]
+                    ].collect { file(it).name }
+                    ).match()
+                }, // md5sum for empty file not allowed in nf-core/tools 2.14.0
+                { assert snapshot(process.out.versions).match("stub_versions") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap
new file mode 100644
index 00000000..ddc9ca06
--- /dev/null
+++ b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap
@@ -0,0 +1,57 @@
+{
+    "bacteroides_fragilis-genome_fna_gz": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.fcs_adaptor_report.txt:md5,27375be4671e01d2d2674ddd1d44414a"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.pipeline_args.yaml:md5,a0cfbaa79a18acf6dc6b67158f448720"
+                ]
+            ],
+            [
+                "versions.yml:md5,5cd44cb2972a42e74144373ce3307c7f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T11:54:31.333434"
+    },
+    "bacteroides_fragilis-genome_fna_gz-stub": {
+        "content": [
+            [
+                "test.fcs_adaptor_report.txt",
+                "test.fcs_adaptor.log",
+                "test.pipeline_args.yaml",
+                "test.skipped_trims.jsonl"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T11:51:58.777173"
+    },
+    "stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,5cd44cb2972a42e74144373ce3307c7f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T11:45:31.030126"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/fcs/fcsadaptor/tests/tags.yml b/modules/nf-core/fcs/fcsadaptor/tests/tags.yml
new file mode 100644
index 00000000..0e774e58
--- /dev/null
+++ b/modules/nf-core/fcs/fcsadaptor/tests/tags.yml
@@ -0,0 +1,2 @@
+fcs/fcsadaptor:
+  - "modules/nf-core/fcs/fcsadaptor/**"
diff --git a/tests/minimal/params.json b/tests/minimal/params.json
new file mode 100644
index 00000000..c5683622
--- /dev/null
+++ b/tests/minimal/params.json
@@ -0,0 +1,8 @@
+{
+    "config_profile_name": "Test profile",
+    "config_profile_description": "Minimal test dataset to check pipeline function",
+    "input": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv",
+    "max_cpus": 2,
+    "max_memory": "6.GB",
+    "max_time": "6.h"
+}
diff --git a/tests/stub/params.json b/tests/stub/params.json
new file mode 100644
index 00000000..4f11914c
--- /dev/null
+++ b/tests/stub/params.json
@@ -0,0 +1,27 @@
+{
+    "config_profile_name": "Full stub test",
+    "config_profile_description": "Full test of the pipeline in stub mode",
+    "input": "assets/assemblysheetv2.csv",
+    "ncbi_fcs_adaptor_skip": false,
+    "ncbi_fcs_adaptor_empire": "euk",
+    "ncbi_fcs_gx_skip": false,
+    "ncbi_fcs_gx_tax_id": 12,
+    "ncbi_fcs_gx_db_path": "tests/stub/gxdb/test",
+    "busco_skip": false,
+    "busco_mode": "genome",
+    "busco_lineage_datasets": "fungi_odb10 hypocreales_odb10",
+    "tidk_skip": false,
+    "tidk_repeat_seq": "TTTGGG",
+    "lai_skip": false,
+    "kraken2_skip": false,
+    "kraken2_db_path": "tests/stub/kraken2/k2_minusb_20231009.tar.gz",
+    "hic": "tests/stub/hic/Dummy_hic.R{1,2}.fq.gz",
+    "hic_skip_fastp": true,
+    "hic_skip_fastqc": false,
+    "synteny_skip": false,
+    "synteny_xref_assemblies": "assets/xrefsheet.csv",
+    "merqury_skip": true,
+    "max_cpus": 2,
+    "max_memory": "6.GB",
+    "max_time": "6.h"
+}
diff --git a/tests/stub/stub.config b/tests/stub/stub.config
deleted file mode 100644
index 7e300f59..00000000
--- a/tests/stub/stub.config
+++ /dev/null
@@ -1,37 +0,0 @@
-params {
-    config_profile_name         = 'Full stub test'
-    config_profile_description  = 'Full test of the pipeline in stub mode'
-
-    input                       = 'assets/assemblysheet.csv'
-
-    ncbi_fcs_adaptor_skip       = false
-    ncbi_fcs_adaptor_empire     = 'euk'
-
-    ncbi_fcs_gx_skip            = false
-    ncbi_fcs_gx_tax_id          = 12
-    ncbi_fcs_gx_db_path         = 'tests/stub/gxdb/test'
-
-    busco_skip                  = false
-    busco_mode                  = 'genome'
-    busco_lineage_datasets      = 'fungi_odb10 hypocreales_odb10'
-
-    tidk_skip                   = false
-    tidk_repeat_seq             = 'TTTGGG'
-
-    lai_skip                    = false
-
-    kraken2_skip                = false
-    kraken2_db_path             = 'tests/stub/kraken2/k2_minusb_20231009.tar.gz'
-
-    hic                         = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz'
-    hic_skip_fastp              = true
-    hic_skip_fastqc             = true
-
-    synteny_skip                = true // GitHub action runner runs out of memory
-    synteny_xref_assemblies     = 'assets/xrefsheet.csv'
-
-    // Limit resources so that this can run on GitHub Actions
-    max_cpus                    = 2
-    max_memory                  = '6.GB'
-    max_time                    = '6.h'
-}
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 2d8dd5b0..ea2729d7 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -12,7 +12,7 @@ include { FASTAVALIDATOR                    } from '../modules/nf-core/fastavali
 include { FASTA_EXPLORE_SEARCH_PLOT_TIDK    } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main'
 
 include { GFF3_GT_GFF3_GFF3VALIDATOR_STAT   } from '../subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main'
-include { NCBI_FCS_ADAPTOR                  } from '../modules/local/ncbi_fcs_adaptor'
+include { FCS_FCSADAPTOR                    } from '../modules/nf-core/fcs/fcsadaptor/main'
 include { NCBI_FCS_GX                       } from '../subworkflows/local/ncbi_fcs_gx'
 include { ASSEMBLATHON_STATS                } from '../modules/local/assemblathon_stats'
 include { FASTA_GXF_BUSCO_PLOT              } from '../subworkflows/pfr/fasta_gxf_busco_plot/main'
@@ -149,54 +149,50 @@ workflow ASSEMBLYQC {
 
     ch_versions                             = ch_versions.mix(GFF3_GT_GFF3_GFF3VALIDATOR_STAT.out.versions)
 
-    // MODULE: NCBI_FCS_ADAPTOR
-    ch_fcs_adaptor_inputs                   = params.ncbi_fcs_adaptor_skip
+    // MODULE: FCS_FCSADAPTOR
+    ch_fcs_adaptor_input                    = params.ncbi_fcs_adaptor_skip
                                             ? Channel.empty()
                                             : ch_valid_target_assembly
-                                            | map { meta, fa -> [ meta.id, fa ] }
 
-    NCBI_FCS_ADAPTOR(
-        ch_fcs_adaptor_inputs,
-        params.ncbi_fcs_adaptor_empire ?: []
+    FCS_FCSADAPTOR(
+        ch_fcs_adaptor_input
     )
 
-    ch_fcs_adaptor_report                   = NCBI_FCS_ADAPTOR.out.report
-                                            | map { tag, report ->
+    ch_fcs_adaptor_report                   = FCS_FCSADAPTOR.out.adaptor_report
+                                            | map { meta, report ->
                                                 def is_clean = file(report).readLines().size < 2
 
                                                 if (! is_clean) {
                                                     log.warn("""
-                                                    Adaptor contamination detected in ${tag}.
+                                                    Adaptor contamination detected in ${meta.id}.
                                                     See the report for further details.
                                                     """.stripIndent())
                                                 }
 
-                                                [ tag, report ]
+                                                [ meta, report ]
                                             }
 
     ch_fcs_adaptor_passed_assembly          = params.ncbi_fcs_adaptor_skip
                                             ? (
                                                 ch_valid_target_assembly
-                                                | map { meta, fa -> [ meta.id, fa ] }
                                             )
                                             : (
                                                 ch_fcs_adaptor_report
-                                                | map { tag, report ->
-                                                    [ tag, file(report).readLines().size < 2 ]
+                                                | map { meta, report ->
+                                                    [ meta, file(report).readLines().size < 2 ]
                                                 }
-                                                | filter { tag, is_clean ->
+                                                | filter { meta, is_clean ->
                                                     ( is_clean || ( ! params.contamination_stops_pipeline ) )
                                                 }
                                                 | join(
                                                     ch_valid_target_assembly
-                                                    | map { meta, fa -> [ meta.id, fa ] }
                                                 )
-                                                | map { tag, clean, fa ->
-                                                    [ tag, fa ]
+                                                | map { meta, clean, fa ->
+                                                    [ meta, fa ]
                                                 }
                                             )
 
-    ch_versions                             = ch_versions.mix(NCBI_FCS_ADAPTOR.out.versions.first())
+    ch_versions                             = ch_versions.mix(FCS_FCSADAPTOR.out.versions.first())
 
     // SUBWORKFLOW: NCBI_FCS_GX
     ch_fcs_gx_input_assembly                = params.ncbi_fcs_gx_skip
@@ -252,6 +248,7 @@ workflow ASSEMBLYQC {
     ch_versions                             = ch_versions.mix(NCBI_FCS_GX.out.versions)
 
     ch_clean_assembly                       = ch_fcs_adaptor_passed_assembly
+                                            | map { meta, fa -> [ meta.id, fa ] }
                                             | join(
                                                 ch_fcs_gx_passed_assembly
                                             )

From 1e57c36b4ac076ba020e58cd98f6db3b971341f6 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 17 May 2024 22:37:51 +1200
Subject: [PATCH 42/76] Added mixed2x test for MERQURY.FK

---
 CHANGELOG.md                               |   2 +-
 docs/images/spectra_cn.png                 | Bin 90113 -> 122391 bytes
 docs/usage.md                              |  11 ++++++++---
 tests/merqury.fk/mixed2x/assemblysheet.csv |   2 ++
 tests/merqury.fk/mixed2x/params.json       |  10 ++++++++++
 5 files changed, 21 insertions(+), 4 deletions(-)
 create mode 100644 tests/merqury.fk/mixed2x/assemblysheet.csv
 create mode 100644 tests/merqury.fk/mixed2x/params.json

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 0488e6be..f44d186a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0+dev - [16-May-2024]
+## v2.0.0+dev - [17-May-2024]
 
 ### `Added`
 
diff --git a/docs/images/spectra_cn.png b/docs/images/spectra_cn.png
index 1a4951571e7d737782e18e35feb8fd4e516bb113..9c6452fae3098824818e59c715056f3eb007d312 100644
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diff --git a/docs/usage.md b/docs/usage.md
index 663d7e9f..9fc62733 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -2,16 +2,21 @@
 
 ## Assemblysheet input
 
-You will need to create an assemblysheet with information about the assemblies you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 5 columns, and a header row. An [example assemblysheet](../assets/assemblysheet.csv) has been provided with the pipeline. Its fields are:
+You will need to create an assemblysheet with information about the assemblies you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 5 columns, and a header row. An [example assemblysheet](../assets/assemblysheetv2.csv) has been provided with the pipeline. Its fields are:
 
 - `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report
 - `fasta:` FASTA file
 - `gff3 [Optional]:` GFF3 annotation file if available
 - `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
 - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) when performing synteny analysis
-- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of a genome by MERQURY.FK.
+- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY.FK.
 - `reads_2 [Optional]`: If `reads_1` is a SRA ID, this column is ignored. Otherwise, this column should list the second file of the paired reads.
 
+See following assemblysheet examples for MERQURY.FK analysis.
+
+- [assemblysheet - 1x](../assets/assemblysheetv2.csv)
+- [assemblysheet - mixed2x](../tests/merqury.fk/mixed2x/assemblysheet.csv)
+
 ## External databases
 
 ### NCBI FCS GX database
@@ -85,7 +90,7 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
   - `fasta:` FASTA file
   - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column)
 
-### Merqury K-mer analysis
+### Merqury.FK K-mer analysis
 
 - `merqury_kmer_length`: kmer length for merqury analysis
 
diff --git a/tests/merqury.fk/mixed2x/assemblysheet.csv b/tests/merqury.fk/mixed2x/assemblysheet.csv
new file mode 100644
index 00000000..61df3863
--- /dev/null
+++ b/tests/merqury.fk/mixed2x/assemblysheet.csv
@@ -0,0 +1,2 @@
+tag,fasta,reads_1
+Col0Cvi0F1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/753/755/GCA_001753755.2_Athal_Col0Cvi0F1_phased_diploid_1.0/GCA_001753755.2_Athal_Col0Cvi0F1_phased_diploid_1.0_genomic.fna.gz,SRR3703081
diff --git a/tests/merqury.fk/mixed2x/params.json b/tests/merqury.fk/mixed2x/params.json
new file mode 100644
index 00000000..06388889
--- /dev/null
+++ b/tests/merqury.fk/mixed2x/params.json
@@ -0,0 +1,10 @@
+{
+    "config_profile_name": "Test profile",
+    "config_profile_description": "Merqury.FK test for a mixed diploid assembly contained in a single fasta",
+    "input": "tests/merqury.fk/mixed2x/assemblysheet.csv",
+    "merqury_skip": false,
+    "merqury_kmer_length": 21,
+    "max_cpus": 8,
+    "max_memory": "32.GB",
+    "max_time": "6.h"
+}

From 56777e050460cf56e71f6e6f0eab1263b1a8254f Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Sat, 18 May 2024 20:36:24 +1200
Subject: [PATCH 43/76] Added module stubs to enable merquryfk in stub test

---
 CHANGELOG.md                                  |   18 +-
 conf/modules.config                           |    8 -
 modules.json                                  |   12 +-
 modules/nf-core/fastk/fastk/environment.yml   |    2 +
 modules/nf-core/fastk/fastk/fastk-fastk.diff  |  542 +++++++++
 modules/nf-core/fastk/fastk/main.nf           |   30 +
 modules/nf-core/fastk/fastk/meta.yml          |    3 +-
 .../nf-core/fastk/fastk/tests/main.nf.test    |  110 ++
 .../fastk/fastk/tests/main.nf.test.snap       |  346 ++++++
 .../nf-core/fastk/fastk/tests/nextflow.config |    3 +
 modules/nf-core/fastk/fastk/tests/tags.yml    |    2 +
 .../merquryfk/merquryfk/environment.yml       |    2 +
 modules/nf-core/merquryfk/merquryfk/main.nf   |   41 +
 .../merquryfk/merquryfk-merquryfk.diff        | 1060 +++++++++++++++++
 modules/nf-core/merquryfk/merquryfk/meta.yml  |    3 +-
 .../merquryfk/merquryfk/tests/main.nf.test    |  153 +++
 .../merquryfk/tests/main.nf.test.snap         |  802 +++++++++++++
 .../merquryfk/merquryfk/tests/nextflow.config |   11 +
 .../merquryfk/merquryfk/tests/tags.yml        |    2 +
 modules/nf-core/sratools/fasterqdump/main.nf  |   50 +-
 modules/nf-core/sratools/fasterqdump/meta.yml |    1 +
 .../fasterqdump/sratools-fasterqdump.diff     |  252 ++++
 .../sratools/fasterqdump/tests/main.nf.test   |   51 +-
 .../fasterqdump/tests/main.nf.test.snap       |   84 +-
 .../fasterqdump/tests/nextflow.config         |    5 -
 modules/nf-core/sratools/prefetch/main.nf     |   12 +
 modules/nf-core/sratools/prefetch/meta.yml    |    1 +
 .../sratools/prefetch/sratools-prefetch.diff  |  112 ++
 .../sratools/prefetch/tests/main.nf.test      |   22 +
 .../sratools/prefetch/tests/main.nf.test.snap |   41 +-
 tests/stub/params.json                        |    2 +-
 31 files changed, 3747 insertions(+), 36 deletions(-)
 create mode 100644 modules/nf-core/fastk/fastk/fastk-fastk.diff
 create mode 100644 modules/nf-core/fastk/fastk/tests/main.nf.test
 create mode 100644 modules/nf-core/fastk/fastk/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/fastk/fastk/tests/nextflow.config
 create mode 100644 modules/nf-core/fastk/fastk/tests/tags.yml
 create mode 100644 modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff
 create mode 100644 modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
 create mode 100644 modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
 create mode 100644 modules/nf-core/merquryfk/merquryfk/tests/tags.yml
 create mode 100644 modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff
 delete mode 100644 modules/nf-core/sratools/fasterqdump/tests/nextflow.config
 create mode 100644 modules/nf-core/sratools/prefetch/sratools-prefetch.diff

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f44d186a..f25f4641 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,18 +11,18 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 2. Removed release-announcements GitHub workflow
 3. Added a list of nf-core contributors
 4. Added a launcher script for local testing `local_assemblyqc`
-5. Added a custom `BUNDLELINKS` module which respects direction when bundling `dnadiff` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
+5. Added a custom `BUNDLELINKS` module which respects direction when bundling `DNADIFF` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
 6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
-7. Updated modules and sub-workflows: `bwa/index`, `bwa/mem`, `cat/cat`, `custom/checkgff3fastacorrespondence`, `custom/restoregffids`, `custom/shortenfastaids`, `gt/gff3`, `gt/gff3validator`, `gt/stat`, `ltrfinder`, `ltrharvest`, `ltrretriever/lai`, `ltrretriever/ltrretriever`, `samblaster`, `fasta_ltrretriever_lai`, `fastq_bwa_mem_samblaster`, `gff3_validate`, `custom/sratoolsncbisettings`, `fastp`, `fastqc`, `untar`, `seqkit/seq`, `seqkit/sort`, `fasta_explore_search_plot_tidk`
+7. Updated modules and sub-workflows: `BWA/INDEX`, `BWA/MEM`, `CAT/CAT`, `CUSTOM/CHECKGFF3FASTACORRESPONDENCE`, `CUSTOM/RESTOREGFFIDS`, `CUSTOM/SHORTENFASTAIDS`, `GT/GFF3`, `GT/GFF3VALIDATOR`, `GT/STAT`, `LTRFINDER`, `LTRHARVEST`, `LTRRETRIEVER/LAI`, `LTRRETRIEVER/LTRRETRIEVER`, `SAMBLASTER`, `FASTA_LTRRETRIEVER_LAI`, `FASTQ_BWA_MEM_SAMBLASTER`, `GFF3_VALIDATE`, `CUSTOM/SRATOOLSNCBISETTINGS`, `FASTP`, `FASTQC`, `UNTAR`, `SEQKIT/SEQ`, `SEQKIT/SORT`, `FASTA_EXPLORE_SEARCH_PLOT_TIDK`
 8. Locally bumped `bwa/index` version to `biocontainers/bwa:0.7.18--he4a0461_0`
 9. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
-10. Updated `fcs-adaptor` to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
-11. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
-12. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
-13. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
-14. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
-15. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
-16. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module
+10. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
+11. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
+12. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
+13. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
+14. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
+15. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
+16. Locally added stubs for `FASTK/FASTK`, `MERQURYFK/MERQURYFK`, `SRATOOLS/FASTERQDUMP` and `SRATOOLS/PREFETCH`
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index e63a0cdb..7910d0cc 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -245,10 +245,6 @@ process {
         ]
     }
 
-    withName: '.*:DOWNLOAD_HIC:SRATOOLS_FASTERQDUMP' {
-        ext.prefix = { "${meta.id}.sra.fastq" }
-    }
-
     withName: FASTQC_RAW {
         publishDir = [
             path: { "${params.outdir}/hic/fastqc_raw" },
@@ -332,10 +328,6 @@ process {
         ext.prefix = { "${meta.id}.fasta" }
     }
 
-    withName: '.*:DOWNLOAD_READS:SRATOOLS_FASTERQDUMP' {
-        ext.prefix = { "${meta.id}.sra.fastq" }
-    }
-
     withName: FASTK_FASTK {
         ext.args = "-k$params.merqury_kmer_length -t1"
     }
diff --git a/modules.json b/modules.json
index d2896927..f16ccf45 100644
--- a/modules.json
+++ b/modules.json
@@ -133,7 +133,8 @@
                     "fastk/fastk": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/fastk/fastk/fastk-fastk.diff"
                     },
                     "fastp": {
                         "branch": "master",
@@ -158,7 +159,8 @@
                     "merquryfk/merquryfk": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff"
                     },
                     "seqkit/seq": {
                         "branch": "master",
@@ -173,12 +175,14 @@
                     "sratools/fasterqdump": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"]
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"],
+                        "patch": "modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff"
                     },
                     "sratools/prefetch": {
                         "branch": "master",
                         "git_sha": "1fc29f92e439d5631fdf34b8ac4687297d70f5ec",
-                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"]
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"],
+                        "patch": "modules/nf-core/sratools/prefetch/sratools-prefetch.diff"
                     },
                     "tidk/explore": {
                         "branch": "master",
diff --git a/modules/nf-core/fastk/fastk/environment.yml b/modules/nf-core/fastk/fastk/environment.yml
index 54c932fe..efb503e1 100644
--- a/modules/nf-core/fastk/fastk/environment.yml
+++ b/modules/nf-core/fastk/fastk/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::false_flag # False flag to pass nf-core/lint
diff --git a/modules/nf-core/fastk/fastk/fastk-fastk.diff b/modules/nf-core/fastk/fastk/fastk-fastk.diff
new file mode 100644
index 00000000..ac5421bd
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/fastk-fastk.diff
@@ -0,0 +1,542 @@
+Changes in module 'nf-core/fastk/fastk'
+--- modules/nf-core/fastk/fastk/environment.yml
++++ modules/nf-core/fastk/fastk/environment.yml
+@@ -3,3 +3,5 @@
+   - conda-forge
+   - bioconda
+   - defaults
++dependencies:
++  - bioconda::false_flag # False flag to pass nf-core/lint
+
+--- modules/nf-core/fastk/fastk/meta.yml
++++ modules/nf-core/fastk/fastk/meta.yml
+@@ -9,7 +9,7 @@
+       description: "A fast K-mer counter for high-fidelity shotgun datasets"
+       homepage: "https://github.com/thegenemyers/FASTK"
+       tool_dev_url: "https://github.com/thegenemyers/FASTK"
+-      licence: "https://github.com/thegenemyers/FASTK/blob/master/LICENSE"
++      licence: ["https://github.com/thegenemyers/FASTK/blob/master/LICENSE"]
+ input:
+   - meta:
+       type: map
+@@ -47,3 +47,4 @@
+   - "@mahesh-panchal"
+ maintainers:
+   - "@mahesh-panchal"
++  - "@gallvp"
+
+--- modules/nf-core/fastk/fastk/main.nf
++++ modules/nf-core/fastk/fastk/main.nf
+@@ -38,4 +38,34 @@
+         fastk: $FASTK_VERSION
+     END_VERSIONS
+     """
++
++    stub:
++    // Exit if running this module with -profile conda / -profile mamba
++    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
++        error "FASTK_FASTK module does not support Conda. Please use Docker / Singularity / Podman instead."
++    }
++    def args = task.ext.args ?: ''
++    def prefix = task.ext.prefix ?: "${meta.id}"
++    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
++
++    def touch_ktab = args.contains('-t') ? "touch ${prefix}_fk.ktab .${prefix}_fk.ktab.1" : ''
++    def touch_prof = args.contains('-p') ? "touch ${prefix}_fk.prof .${prefix}_fk.pidx.1" : ''
++    """
++    touch ${prefix}_fk.hist
++    $touch_ktab
++    $touch_prof
++
++    echo \\
++    "FastK \\
++        $args \\
++        -T$task.cpus \\
++        -M${task.memory.toGiga()} \\
++        -N${prefix}_fk \\
++        $reads"
++
++    cat <<-END_VERSIONS > versions.yml
++    "${task.process}":
++        fastk: $FASTK_VERSION
++    END_VERSIONS
++    """
+ }
+
+--- /dev/null
++++ modules/nf-core/fastk/fastk/tests/main.nf.test.snap
+@@ -0,0 +1,346 @@
++{
++    "test_fastk_fastk_single_end_stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "3": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ],
++                "hist": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "ktab": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "prof": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T19:39:28.510263"
++    },
++    "test_fastk_fastk_single_end": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
++                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
++                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
++                        ]
++                    ]
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
++                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
++                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
++                        ]
++                    ]
++                ],
++                "3": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ],
++                "hist": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
++                    ]
++                ],
++                "ktab": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
++                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
++                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
++                        ]
++                    ]
++                ],
++                "prof": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
++                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
++                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
++                        ]
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T19:31:31.128388"
++    },
++    "test_fastk_fastk_paired_end": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
++                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
++                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
++                        ]
++                    ]
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
++                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
++                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
++                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
++                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
++                        ]
++                    ]
++                ],
++                "3": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ],
++                "hist": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
++                    ]
++                ],
++                "ktab": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
++                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
++                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
++                        ]
++                    ]
++                ],
++                "prof": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
++                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
++                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
++                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
++                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
++                        ]
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T19:31:35.565502"
++    },
++    "test_fastk_fastk_paired_end_stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "3": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ],
++                "hist": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "ktab": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "prof": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T19:39:32.570957"
++    }
++}
+--- /dev/null
++++ modules/nf-core/fastk/fastk/tests/tags.yml
+@@ -0,0 +1,2 @@
++fastk/fastk:
++  - "modules/nf-core/fastk/fastk/**"
+
+--- /dev/null
++++ modules/nf-core/fastk/fastk/tests/nextflow.config
+@@ -0,0 +1,3 @@
++process {
++    ext.args = '-t -p'
++}
+
+--- /dev/null
++++ modules/nf-core/fastk/fastk/tests/main.nf.test
+@@ -0,0 +1,110 @@
++nextflow_process {
++
++    name "Test Process FASTK_FASTK"
++    script "../main.nf"
++    config './nextflow.config'
++    process "FASTK_FASTK"
++
++    tag "modules"
++    tag "modules_nfcore"
++    tag "fastk"
++    tag "fastk/fastk"
++
++    test("test_fastk_fastk_single_end") {
++        when {
++            process {
++                """
++                input[0] = [
++                    [ id:'test' , single_end: true ], // meta map
++                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
++                ]
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++
++    }
++
++    test("test_fastk_fastk_paired_end") {
++
++        when {
++            process {
++                """
++                input[0] = [
++                    [ id:'test' , single_end: false ], // meta map
++                    [
++                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
++                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
++                    ]
++                ]
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++
++    }
++
++    test("test_fastk_fastk_single_end_stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                input[0] = [
++                    [ id:'test' , single_end: true ], // meta map
++                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
++                ]
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++
++    }
++
++    test("test_fastk_fastk_paired_end_stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                input[0] = [
++                    [ id:'test' , single_end: false ], // meta map
++                    [
++                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
++                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
++                    ]
++                ]
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++
++    }
++
++}
+
+************************************************************
diff --git a/modules/nf-core/fastk/fastk/main.nf b/modules/nf-core/fastk/fastk/main.nf
index fec5a4d2..cef1a3c8 100644
--- a/modules/nf-core/fastk/fastk/main.nf
+++ b/modules/nf-core/fastk/fastk/main.nf
@@ -38,4 +38,34 @@ process FASTK_FASTK {
         fastk: $FASTK_VERSION
     END_VERSIONS
     """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "FASTK_FASTK module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+
+    def touch_ktab = args.contains('-t') ? "touch ${prefix}_fk.ktab .${prefix}_fk.ktab.1" : ''
+    def touch_prof = args.contains('-p') ? "touch ${prefix}_fk.prof .${prefix}_fk.pidx.1" : ''
+    """
+    touch ${prefix}_fk.hist
+    $touch_ktab
+    $touch_prof
+
+    echo \\
+    "FastK \\
+        $args \\
+        -T$task.cpus \\
+        -M${task.memory.toGiga()} \\
+        -N${prefix}_fk \\
+        $reads"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/fastk/fastk/meta.yml b/modules/nf-core/fastk/fastk/meta.yml
index 7c7f4260..3086aca5 100644
--- a/modules/nf-core/fastk/fastk/meta.yml
+++ b/modules/nf-core/fastk/fastk/meta.yml
@@ -9,7 +9,7 @@ tools:
       description: "A fast K-mer counter for high-fidelity shotgun datasets"
       homepage: "https://github.com/thegenemyers/FASTK"
       tool_dev_url: "https://github.com/thegenemyers/FASTK"
-      licence: "https://github.com/thegenemyers/FASTK/blob/master/LICENSE"
+      licence: ["https://github.com/thegenemyers/FASTK/blob/master/LICENSE"]
 input:
   - meta:
       type: map
@@ -47,3 +47,4 @@ authors:
   - "@mahesh-panchal"
 maintainers:
   - "@mahesh-panchal"
+  - "@gallvp"
diff --git a/modules/nf-core/fastk/fastk/tests/main.nf.test b/modules/nf-core/fastk/fastk/tests/main.nf.test
new file mode 100644
index 00000000..e46d8bce
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/tests/main.nf.test
@@ -0,0 +1,110 @@
+nextflow_process {
+
+    name "Test Process FASTK_FASTK"
+    script "../main.nf"
+    config './nextflow.config'
+    process "FASTK_FASTK"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "fastk"
+    tag "fastk/fastk"
+
+    test("test_fastk_fastk_single_end") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' , single_end: true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("test_fastk_fastk_paired_end") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' , single_end: false ], // meta map
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("test_fastk_fastk_single_end_stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' , single_end: true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("test_fastk_fastk_paired_end_stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' , single_end: false ], // meta map
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/fastk/fastk/tests/main.nf.test.snap b/modules/nf-core/fastk/fastk/tests/main.nf.test.snap
new file mode 100644
index 00000000..1e3fc4da
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/tests/main.nf.test.snap
@@ -0,0 +1,346 @@
+{
+    "test_fastk_fastk_single_end_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ],
+                "hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ktab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "prof": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T19:39:28.510263"
+    },
+    "test_fastk_fastk_single_end": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
+                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
+                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
+                        ]
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
+                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
+                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
+                        ]
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ],
+                "hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
+                    ]
+                ],
+                "ktab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
+                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
+                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
+                        ]
+                    ]
+                ],
+                "prof": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
+                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
+                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T19:31:31.128388"
+    },
+    "test_fastk_fastk_paired_end": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
+                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
+                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
+                        ]
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
+                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
+                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
+                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
+                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
+                        ]
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ],
+                "hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
+                    ]
+                ],
+                "ktab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
+                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
+                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
+                        ]
+                    ]
+                ],
+                "prof": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
+                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
+                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
+                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
+                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T19:31:35.565502"
+    },
+    "test_fastk_fastk_paired_end_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ],
+                "hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ktab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "prof": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T19:39:32.570957"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/fastk/fastk/tests/nextflow.config b/modules/nf-core/fastk/fastk/tests/nextflow.config
new file mode 100644
index 00000000..c89ce9d5
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/tests/nextflow.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = '-t -p'
+}
diff --git a/modules/nf-core/fastk/fastk/tests/tags.yml b/modules/nf-core/fastk/fastk/tests/tags.yml
new file mode 100644
index 00000000..82f9df82
--- /dev/null
+++ b/modules/nf-core/fastk/fastk/tests/tags.yml
@@ -0,0 +1,2 @@
+fastk/fastk:
+  - "modules/nf-core/fastk/fastk/**"
diff --git a/modules/nf-core/merquryfk/merquryfk/environment.yml b/modules/nf-core/merquryfk/merquryfk/environment.yml
index 44a5ee9b..325bb013 100644
--- a/modules/nf-core/merquryfk/merquryfk/environment.yml
+++ b/modules/nf-core/merquryfk/merquryfk/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::false_flag # False flag to pass nf-core/lint
diff --git a/modules/nf-core/merquryfk/merquryfk/main.nf b/modules/nf-core/merquryfk/merquryfk/main.nf
index ac163dac..f22e4431 100644
--- a/modules/nf-core/merquryfk/merquryfk/main.nf
+++ b/modules/nf-core/merquryfk/merquryfk/main.nf
@@ -55,4 +55,45 @@ process MERQURYFK_MERQURYFK {
         r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
     END_VERSIONS
     """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+
+    def touch_asm_bed   = "touch ${prefix}.${assembly.baseName}_only.bed"
+    def touch_asm_qv    = "touch ${prefix}.${assembly.baseName}.qv"
+    def touch_haplo_bed = haplotigs ? "touch ${prefix}.${haplotigs.baseName}_only.bed" : ''
+    def touch_haplo_qv  = haplotigs ? "touch ${prefix}.${haplotigs.baseName}.qv" : ''
+    """
+    touch "${prefix}.completeness.stats"
+
+    $touch_asm_bed
+    $touch_asm_qv
+    $touch_haplo_bed
+    $touch_haplo_qv
+
+    touch "${prefix}.qv"
+
+    echo \\
+    "MerquryFK \\
+        $args \\
+        -T$task.cpus \\
+        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }} \\
+        $assembly \\
+        $haplotigs \\
+        $prefix"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+        merquryfk: $MERQURY_VERSION
+        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff b/modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff
new file mode 100644
index 00000000..b630c090
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff
@@ -0,0 +1,1060 @@
+Changes in module 'nf-core/merquryfk/merquryfk'
+--- modules/nf-core/merquryfk/merquryfk/environment.yml
++++ modules/nf-core/merquryfk/merquryfk/environment.yml
+@@ -3,3 +3,5 @@
+   - conda-forge
+   - bioconda
+   - defaults
++dependencies:
++  - bioconda::false_flag # False flag to pass nf-core/lint
+
+--- modules/nf-core/merquryfk/merquryfk/meta.yml
++++ modules/nf-core/merquryfk/merquryfk/meta.yml
+@@ -9,7 +9,7 @@
+       description: "FastK based version of Merqury"
+       homepage: "https://github.com/thegenemyers/MERQURY.FK"
+       tool_dev_url: "https://github.com/thegenemyers/MERQURY.FK"
+-      licence: "https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"
++      licence: ["https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"]
+ input:
+   - meta:
+       type: map
+@@ -110,3 +110,4 @@
+   - "@mahesh-panchal"
+ maintainers:
+   - "@mahesh-panchal"
++  - "@gallvp"
+
+--- modules/nf-core/merquryfk/merquryfk/main.nf
++++ modules/nf-core/merquryfk/merquryfk/main.nf
+@@ -55,4 +55,45 @@
+         r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
+     END_VERSIONS
+     """
++
++    stub:
++    // Exit if running this module with -profile conda / -profile mamba
++    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
++        error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
++    }
++    def args = task.ext.args ?: ''
++    prefix = task.ext.prefix ?: "${meta.id}"
++    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
++    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
++
++    def touch_asm_bed   = "touch ${prefix}.${assembly.baseName}_only.bed"
++    def touch_asm_qv    = "touch ${prefix}.${assembly.baseName}.qv"
++    def touch_haplo_bed = haplotigs ? "touch ${prefix}.${haplotigs.baseName}_only.bed" : ''
++    def touch_haplo_qv  = haplotigs ? "touch ${prefix}.${haplotigs.baseName}.qv" : ''
++    """
++    touch "${prefix}.completeness.stats"
++
++    $touch_asm_bed
++    $touch_asm_qv
++    $touch_haplo_bed
++    $touch_haplo_qv
++
++    touch "${prefix}.qv"
++
++    echo \\
++    "MerquryFK \\
++        $args \\
++        -T$task.cpus \\
++        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }} \\
++        $assembly \\
++        $haplotigs \\
++        $prefix"
++
++    cat <<-END_VERSIONS > versions.yml
++    "${task.process}":
++        fastk: $FASTK_VERSION
++        merquryfk: $MERQURY_VERSION
++        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
++    END_VERSIONS
++    """
+ }
+
+--- /dev/null
++++ modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
+@@ -0,0 +1,802 @@
++{
++    "multiple_minimal_stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "10": [
++                    
++                ],
++                "11": [
++                    
++                ],
++                "12": [
++                    
++                ],
++                "13": [
++                    
++                ],
++                "14": [
++                    
++                ],
++                "15": [
++                    
++                ],
++                "16": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "3": [
++                    
++                ],
++                "4": [
++                    
++                ],
++                "5": [
++                    
++                ],
++                "6": [
++                    
++                ],
++                "7": [
++                    
++                ],
++                "8": [
++                    
++                ],
++                "9": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "assembly_qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "bed": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        [
++                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "spectra_asm_fl_pdf": [
++                    
++                ],
++                "spectra_asm_fl_png": [
++                    
++                ],
++                "spectra_asm_ln_pdf": [
++                    
++                ],
++                "spectra_asm_ln_png": [
++                    
++                ],
++                "spectra_asm_st_pdf": [
++                    
++                ],
++                "spectra_asm_st_png": [
++                    
++                ],
++                "spectra_cn_fl_pdf": [
++                    
++                ],
++                "spectra_cn_fl_png": [
++                    
++                ],
++                "spectra_cn_ln_pdf": [
++                    
++                ],
++                "spectra_cn_ln_png": [
++                    
++                ],
++                "spectra_cn_st_pdf": [
++                    
++                ],
++                "spectra_cn_st_png": [
++                    
++                ],
++                "stats": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T20:21:30.488412"
++    },
++    "test_merquryfk_merquryfk_pdf": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
++                    ]
++                ],
++                "10": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
++                    ]
++                ],
++                "11": [
++                    
++                ],
++                "12": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
++                    ]
++                ],
++                "13": [
++                    
++                ],
++                "14": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
++                    ]
++                ],
++                "15": [
++                    
++                ],
++                "16": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
++                    ]
++                ],
++                "3": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "4": [
++                    
++                ],
++                "5": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "6": [
++                    
++                ],
++                "7": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "8": [
++                    
++                ],
++                "9": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
++                    ]
++                ],
++                "assembly_qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
++                    ]
++                ],
++                "bed": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
++                    ]
++                ],
++                "qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
++                    ]
++                ],
++                "spectra_asm_fl_pdf": [
++                    
++                ],
++                "spectra_asm_fl_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
++                    ]
++                ],
++                "spectra_asm_ln_pdf": [
++                    
++                ],
++                "spectra_asm_ln_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
++                    ]
++                ],
++                "spectra_asm_st_pdf": [
++                    
++                ],
++                "spectra_asm_st_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
++                    ]
++                ],
++                "spectra_cn_fl_pdf": [
++                    
++                ],
++                "spectra_cn_fl_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "spectra_cn_ln_pdf": [
++                    
++                ],
++                "spectra_cn_ln_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "spectra_cn_st_pdf": [
++                    
++                ],
++                "spectra_cn_st_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "stats": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T19:58:05.545683"
++    },
++    "single_minimal_stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "10": [
++                    
++                ],
++                "11": [
++                    
++                ],
++                "12": [
++                    
++                ],
++                "13": [
++                    
++                ],
++                "14": [
++                    
++                ],
++                "15": [
++                    
++                ],
++                "16": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "3": [
++                    
++                ],
++                "4": [
++                    
++                ],
++                "5": [
++                    
++                ],
++                "6": [
++                    
++                ],
++                "7": [
++                    
++                ],
++                "8": [
++                    
++                ],
++                "9": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "assembly_qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "bed": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "spectra_asm_fl_pdf": [
++                    
++                ],
++                "spectra_asm_fl_png": [
++                    
++                ],
++                "spectra_asm_ln_pdf": [
++                    
++                ],
++                "spectra_asm_ln_png": [
++                    
++                ],
++                "spectra_asm_st_pdf": [
++                    
++                ],
++                "spectra_asm_st_png": [
++                    
++                ],
++                "spectra_cn_fl_pdf": [
++                    
++                ],
++                "spectra_cn_fl_png": [
++                    
++                ],
++                "spectra_cn_ln_pdf": [
++                    
++                ],
++                "spectra_cn_ln_png": [
++                    
++                ],
++                "spectra_cn_st_pdf": [
++                    
++                ],
++                "spectra_cn_st_png": [
++                    
++                ],
++                "stats": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T20:21:19.606389"
++    },
++    "test_merquryfk_merquryfk_png": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
++                    ]
++                ],
++                "1": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
++                    ]
++                ],
++                "10": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
++                    ]
++                ],
++                "11": [
++                    
++                ],
++                "12": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
++                    ]
++                ],
++                "13": [
++                    
++                ],
++                "14": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
++                    ]
++                ],
++                "15": [
++                    
++                ],
++                "16": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ],
++                "2": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
++                    ]
++                ],
++                "3": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "4": [
++                    
++                ],
++                "5": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "6": [
++                    
++                ],
++                "7": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "8": [
++                    
++                ],
++                "9": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
++                    ]
++                ],
++                "assembly_qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
++                    ]
++                ],
++                "bed": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
++                    ]
++                ],
++                "qv": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
++                    ]
++                ],
++                "spectra_asm_fl_pdf": [
++                    
++                ],
++                "spectra_asm_fl_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
++                    ]
++                ],
++                "spectra_asm_ln_pdf": [
++                    
++                ],
++                "spectra_asm_ln_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
++                    ]
++                ],
++                "spectra_asm_st_pdf": [
++                    
++                ],
++                "spectra_asm_st_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
++                    ]
++                ],
++                "spectra_cn_fl_pdf": [
++                    
++                ],
++                "spectra_cn_fl_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "spectra_cn_ln_pdf": [
++                    
++                ],
++                "spectra_cn_ln_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "spectra_cn_st_pdf": [
++                    
++                ],
++                "spectra_cn_st_png": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
++                    ]
++                ],
++                "stats": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": true
++                        },
++                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-18T19:57:31.981156"
++    }
++}
+--- /dev/null
++++ modules/nf-core/merquryfk/merquryfk/tests/tags.yml
+@@ -0,0 +1,2 @@
++merquryfk/merquryfk:
++  - "modules/nf-core/merquryfk/merquryfk/**"
+
+--- /dev/null
++++ modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
+@@ -0,0 +1,11 @@
++process {
++    withName: 'FASTK_.*' {
++        ext.args   = '-t'
++    }
++    withName: 'test_merquryfk_merquryfk_png:MERQURYFK_MERQURYFK' {
++        ext.args   = '-lfs'
++    }
++    withName: 'test_merquryfk_merquryfk_pdf:MERQURYFK_MERQURYFK' {
++        ext.args   = '-lfs -pdf'
++    }
++}
+
+--- /dev/null
++++ modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
+@@ -0,0 +1,153 @@
++nextflow_process {
++
++    name "Test Process MERQURYFK_MERQURYFK"
++    script "../main.nf"
++    config "./nextflow.config"
++    process "MERQURYFK_MERQURYFK"
++
++    tag "modules"
++    tag "modules_nfcore"
++    tag "merquryfk"
++    tag "merquryfk/merquryfk"
++    tag "fastk/fastk"
++
++    setup {
++        run("FASTK_FASTK") {
++            script "../../../fastk/fastk"
++            process {
++                """
++                input[0] =  [
++                    [ id:'test', single_end:true ], // meta map
++                    file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
++                ]
++                """
++            }
++        }
++    }
++
++    test("test_merquryfk_merquryfk_png") {
++
++        when {
++            process {
++                """
++                assembly = [
++                    [ id:'test', single_end:true ], // meta map
++                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
++                ]
++
++                haplotigs = [
++                    [ id:'test', single_end:true ], []
++                ]
++
++                input[0] = FASTK_FASTK.out.hist
++                            | join( FASTK_FASTK.out.ktab )
++                            | join( Channel.value( assembly ) )
++                            | join( Channel.value( haplotigs ) )
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++    }
++
++    test("test_merquryfk_merquryfk_pdf") {
++
++        when {
++            process {
++                """
++                assembly = [
++                    [ id:'test', single_end:true ], // meta map
++                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
++                ]
++
++                haplotigs = [
++                    [ id:'test', single_end:true ], []
++                ]
++
++                input[0] = FASTK_FASTK.out.hist
++                            | join( FASTK_FASTK.out.ktab )
++                            | join( Channel.value( assembly ) )
++                            | join( Channel.value( haplotigs ) )
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++    }
++
++    test("single_minimal_stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                assembly = [
++                    [ id:'test', single_end:true ], // meta map
++                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
++                ]
++
++                haplotigs = [
++                    [ id:'test', single_end:true ], []
++                ]
++
++                input[0] = FASTK_FASTK.out.hist
++                            | join( FASTK_FASTK.out.ktab )
++                            | join( Channel.value( assembly ) )
++                            | join( Channel.value( haplotigs ) )
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++    }
++
++    test("multiple_minimal_stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                assembly = [
++                    [ id:'test', single_end:true ], // meta map
++                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
++                ]
++
++                haplotigs = [
++                    [ id:'test', single_end:true ],
++                    file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
++                ]
++
++                input[0] = FASTK_FASTK.out.hist
++                            | join( FASTK_FASTK.out.ktab )
++                            | join( Channel.value( assembly ) )
++                            | join( Channel.value( haplotigs ) )
++                """
++            }
++        }
++
++        then {
++            assertAll(
++                { assert process.success },
++                { assert snapshot(process.out).match() }
++            )
++        }
++    }
++
++}
+
+************************************************************
diff --git a/modules/nf-core/merquryfk/merquryfk/meta.yml b/modules/nf-core/merquryfk/merquryfk/meta.yml
index 7d4af796..0cdbcae6 100644
--- a/modules/nf-core/merquryfk/merquryfk/meta.yml
+++ b/modules/nf-core/merquryfk/merquryfk/meta.yml
@@ -9,7 +9,7 @@ tools:
       description: "FastK based version of Merqury"
       homepage: "https://github.com/thegenemyers/MERQURY.FK"
       tool_dev_url: "https://github.com/thegenemyers/MERQURY.FK"
-      licence: "https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"
+      licence: ["https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"]
 input:
   - meta:
       type: map
@@ -110,3 +110,4 @@ authors:
   - "@mahesh-panchal"
 maintainers:
   - "@mahesh-panchal"
+  - "@gallvp"
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test b/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
new file mode 100644
index 00000000..5c4cce76
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
@@ -0,0 +1,153 @@
+nextflow_process {
+
+    name "Test Process MERQURYFK_MERQURYFK"
+    script "../main.nf"
+    config "./nextflow.config"
+    process "MERQURYFK_MERQURYFK"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "merquryfk"
+    tag "merquryfk/merquryfk"
+    tag "fastk/fastk"
+
+    setup {
+        run("FASTK_FASTK") {
+            script "../../../fastk/fastk"
+            process {
+                """
+                input[0] =  [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+    }
+
+    test("test_merquryfk_merquryfk_png") {
+
+        when {
+            process {
+                """
+                assembly = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+
+                haplotigs = [
+                    [ id:'test', single_end:true ], []
+                ]
+
+                input[0] = FASTK_FASTK.out.hist
+                            | join( FASTK_FASTK.out.ktab )
+                            | join( Channel.value( assembly ) )
+                            | join( Channel.value( haplotigs ) )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_merquryfk_merquryfk_pdf") {
+
+        when {
+            process {
+                """
+                assembly = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+
+                haplotigs = [
+                    [ id:'test', single_end:true ], []
+                ]
+
+                input[0] = FASTK_FASTK.out.hist
+                            | join( FASTK_FASTK.out.ktab )
+                            | join( Channel.value( assembly ) )
+                            | join( Channel.value( haplotigs ) )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("single_minimal_stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                assembly = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+
+                haplotigs = [
+                    [ id:'test', single_end:true ], []
+                ]
+
+                input[0] = FASTK_FASTK.out.hist
+                            | join( FASTK_FASTK.out.ktab )
+                            | join( Channel.value( assembly ) )
+                            | join( Channel.value( haplotigs ) )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("multiple_minimal_stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                assembly = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+
+                haplotigs = [
+                    [ id:'test', single_end:true ],
+                    file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
+                ]
+
+                input[0] = FASTK_FASTK.out.hist
+                            | join( FASTK_FASTK.out.ktab )
+                            | join( Channel.value( assembly ) )
+                            | join( Channel.value( haplotigs ) )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap b/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
new file mode 100644
index 00000000..eebc609f
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
@@ -0,0 +1,802 @@
+{
+    "multiple_minimal_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "10": [
+                    
+                ],
+                "11": [
+                    
+                ],
+                "12": [
+                    
+                ],
+                "13": [
+                    
+                ],
+                "14": [
+                    
+                ],
+                "15": [
+                    
+                ],
+                "16": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "assembly_qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_asm_fl_pdf": [
+                    
+                ],
+                "spectra_asm_fl_png": [
+                    
+                ],
+                "spectra_asm_ln_pdf": [
+                    
+                ],
+                "spectra_asm_ln_png": [
+                    
+                ],
+                "spectra_asm_st_pdf": [
+                    
+                ],
+                "spectra_asm_st_png": [
+                    
+                ],
+                "spectra_cn_fl_pdf": [
+                    
+                ],
+                "spectra_cn_fl_png": [
+                    
+                ],
+                "spectra_cn_ln_pdf": [
+                    
+                ],
+                "spectra_cn_ln_png": [
+                    
+                ],
+                "spectra_cn_st_pdf": [
+                    
+                ],
+                "spectra_cn_st_png": [
+                    
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T20:21:30.488412"
+    },
+    "test_merquryfk_merquryfk_pdf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
+                    ]
+                ],
+                "11": [
+                    
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
+                    ]
+                ],
+                "13": [
+                    
+                ],
+                "14": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
+                    ]
+                ],
+                "15": [
+                    
+                ],
+                "16": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
+                    ]
+                ],
+                "assembly_qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
+                    ]
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
+                    ]
+                ],
+                "qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
+                    ]
+                ],
+                "spectra_asm_fl_pdf": [
+                    
+                ],
+                "spectra_asm_fl_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
+                    ]
+                ],
+                "spectra_asm_ln_pdf": [
+                    
+                ],
+                "spectra_asm_ln_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
+                    ]
+                ],
+                "spectra_asm_st_pdf": [
+                    
+                ],
+                "spectra_asm_st_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
+                    ]
+                ],
+                "spectra_cn_fl_pdf": [
+                    
+                ],
+                "spectra_cn_fl_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "spectra_cn_ln_pdf": [
+                    
+                ],
+                "spectra_cn_ln_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "spectra_cn_st_pdf": [
+                    
+                ],
+                "spectra_cn_st_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T19:58:05.545683"
+    },
+    "single_minimal_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "10": [
+                    
+                ],
+                "11": [
+                    
+                ],
+                "12": [
+                    
+                ],
+                "13": [
+                    
+                ],
+                "14": [
+                    
+                ],
+                "15": [
+                    
+                ],
+                "16": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "assembly_qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_asm_fl_pdf": [
+                    
+                ],
+                "spectra_asm_fl_png": [
+                    
+                ],
+                "spectra_asm_ln_pdf": [
+                    
+                ],
+                "spectra_asm_ln_png": [
+                    
+                ],
+                "spectra_asm_st_pdf": [
+                    
+                ],
+                "spectra_asm_st_png": [
+                    
+                ],
+                "spectra_cn_fl_pdf": [
+                    
+                ],
+                "spectra_cn_fl_png": [
+                    
+                ],
+                "spectra_cn_ln_pdf": [
+                    
+                ],
+                "spectra_cn_ln_png": [
+                    
+                ],
+                "spectra_cn_st_pdf": [
+                    
+                ],
+                "spectra_cn_st_png": [
+                    
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T20:21:19.606389"
+    },
+    "test_merquryfk_merquryfk_png": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
+                    ]
+                ],
+                "11": [
+                    
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
+                    ]
+                ],
+                "13": [
+                    
+                ],
+                "14": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
+                    ]
+                ],
+                "15": [
+                    
+                ],
+                "16": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
+                    ]
+                ],
+                "assembly_qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
+                    ]
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
+                    ]
+                ],
+                "qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
+                    ]
+                ],
+                "spectra_asm_fl_pdf": [
+                    
+                ],
+                "spectra_asm_fl_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
+                    ]
+                ],
+                "spectra_asm_ln_pdf": [
+                    
+                ],
+                "spectra_asm_ln_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
+                    ]
+                ],
+                "spectra_asm_st_pdf": [
+                    
+                ],
+                "spectra_asm_st_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
+                    ]
+                ],
+                "spectra_cn_fl_pdf": [
+                    
+                ],
+                "spectra_cn_fl_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "spectra_cn_ln_pdf": [
+                    
+                ],
+                "spectra_cn_ln_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "spectra_cn_st_pdf": [
+                    
+                ],
+                "spectra_cn_st_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-18T19:57:31.981156"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/nextflow.config b/modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
new file mode 100644
index 00000000..63d72d24
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
@@ -0,0 +1,11 @@
+process {
+    withName: 'FASTK_.*' {
+        ext.args   = '-t'
+    }
+    withName: 'test_merquryfk_merquryfk_png:MERQURYFK_MERQURYFK' {
+        ext.args   = '-lfs'
+    }
+    withName: 'test_merquryfk_merquryfk_pdf:MERQURYFK_MERQURYFK' {
+        ext.args   = '-lfs -pdf'
+    }
+}
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/tags.yml b/modules/nf-core/merquryfk/merquryfk/tests/tags.yml
new file mode 100644
index 00000000..7dcac99b
--- /dev/null
+++ b/modules/nf-core/merquryfk/merquryfk/tests/tags.yml
@@ -0,0 +1,2 @@
+merquryfk/merquryfk:
+  - "modules/nf-core/merquryfk/merquryfk/**"
diff --git a/modules/nf-core/sratools/fasterqdump/main.nf b/modules/nf-core/sratools/fasterqdump/main.nf
index d4ddf268..d5b65bab 100644
--- a/modules/nf-core/sratools/fasterqdump/main.nf
+++ b/modules/nf-core/sratools/fasterqdump/main.nf
@@ -23,7 +23,10 @@ process SRATOOLS_FASTERQDUMP {
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def outfile = meta.single_end ? "${prefix}.fastq" : prefix
+    def outfile = "${prefix}.fastq"
+    def exclude_third = meta.single_end ? '' : "mv $outfile $prefix || echo 'No third file'"
+    // Excludes the "${prefix}.fastq" file from output `reads` channel for paired end cases and
+    // avoids the '.' in the path bug: https://github.com/ncbi/sra-tools/issues/865
     def key_file = ''
     if (certificate.toString().endsWith('.jwt')) {
         key_file += " --perm ${certificate}"
@@ -40,6 +43,51 @@ process SRATOOLS_FASTERQDUMP {
         ${key_file} \\
         ${sra}
 
+    $exclude_third
+
+    pigz \\
+        $args2 \\
+        --no-name \\
+        --processes $task.cpus \\
+        *.fastq
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sratools: \$(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def outfile = "${prefix}.fastq"
+    def exclude_third = meta.single_end ? '' : "mv $outfile $prefix || echo 'No third file'"
+    // Excludes the "${prefix}.fastq" file from output `reads` channel for paired end cases and
+    // avoids the '.' in the path bug: https://github.com/ncbi/sra-tools/issues/865
+    def key_file = ''
+    if (certificate.toString().endsWith('.jwt')) {
+        key_file += " --perm ${certificate}"
+    } else if (certificate.toString().endsWith('.ngc')) {
+        key_file += " --ngc ${certificate}"
+    }
+    def touch_outfiles = meta.single_end ? "${prefix}.fastq" : "${prefix}_1.fastq ${prefix}_2.fastq"
+    """
+    touch $touch_outfiles
+
+    export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
+
+    echo \\
+    "fasterq-dump \\
+        $args \\
+        --threads $task.cpus \\
+        --outfile $outfile \\
+        ${key_file} \\
+        ${sra}"
+
+    $exclude_third
+
     pigz \\
         $args2 \\
         --no-name \\
diff --git a/modules/nf-core/sratools/fasterqdump/meta.yml b/modules/nf-core/sratools/fasterqdump/meta.yml
index b5e0175a..6a2151a8 100644
--- a/modules/nf-core/sratools/fasterqdump/meta.yml
+++ b/modules/nf-core/sratools/fasterqdump/meta.yml
@@ -51,3 +51,4 @@ authors:
   - "@Midnighter"
 maintainers:
   - "@Midnighter"
+  - "@gallvp"
diff --git a/modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff b/modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff
new file mode 100644
index 00000000..d71c09b1
--- /dev/null
+++ b/modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff
@@ -0,0 +1,252 @@
+Changes in module 'nf-core/sratools/fasterqdump'
+--- modules/nf-core/sratools/fasterqdump/meta.yml
++++ modules/nf-core/sratools/fasterqdump/meta.yml
+@@ -51,3 +51,4 @@
+   - "@Midnighter"
+ maintainers:
+   - "@Midnighter"
++  - "@gallvp"
+
+--- modules/nf-core/sratools/fasterqdump/main.nf
++++ modules/nf-core/sratools/fasterqdump/main.nf
+@@ -23,7 +23,10 @@
+     def args = task.ext.args ?: ''
+     def args2 = task.ext.args2 ?: ''
+     def prefix = task.ext.prefix ?: "${meta.id}"
+-    def outfile = meta.single_end ? "${prefix}.fastq" : prefix
++    def outfile = "${prefix}.fastq"
++    def exclude_third = meta.single_end ? '' : "mv $outfile $prefix || echo 'No third file'"
++    // Excludes the "${prefix}.fastq" file from output `reads` channel for paired end cases and
++    // avoids the '.' in the path bug: https://github.com/ncbi/sra-tools/issues/865
+     def key_file = ''
+     if (certificate.toString().endsWith('.jwt')) {
+         key_file += " --perm ${certificate}"
+@@ -40,6 +43,51 @@
+         ${key_file} \\
+         ${sra}
+ 
++    $exclude_third
++
++    pigz \\
++        $args2 \\
++        --no-name \\
++        --processes $task.cpus \\
++        *.fastq
++
++    cat <<-END_VERSIONS > versions.yml
++    "${task.process}":
++        sratools: \$(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
++        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
++    END_VERSIONS
++    """
++
++    stub:
++    def args = task.ext.args ?: ''
++    def args2 = task.ext.args2 ?: ''
++    def prefix = task.ext.prefix ?: "${meta.id}"
++    def outfile = "${prefix}.fastq"
++    def exclude_third = meta.single_end ? '' : "mv $outfile $prefix || echo 'No third file'"
++    // Excludes the "${prefix}.fastq" file from output `reads` channel for paired end cases and
++    // avoids the '.' in the path bug: https://github.com/ncbi/sra-tools/issues/865
++    def key_file = ''
++    if (certificate.toString().endsWith('.jwt')) {
++        key_file += " --perm ${certificate}"
++    } else if (certificate.toString().endsWith('.ngc')) {
++        key_file += " --ngc ${certificate}"
++    }
++    def touch_outfiles = meta.single_end ? "${prefix}.fastq" : "${prefix}_1.fastq ${prefix}_2.fastq"
++    """
++    touch $touch_outfiles
++
++    export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
++
++    echo \\
++    "fasterq-dump \\
++        $args \\
++        --threads $task.cpus \\
++        --outfile $outfile \\
++        ${key_file} \\
++        ${sra}"
++
++    $exclude_third
++
+     pigz \\
+         $args2 \\
+         --no-name \\
+
+--- modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
++++ modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
+@@ -30,9 +30,44 @@
+         ],
+         "meta": {
+             "nf-test": "0.8.4",
+-            "nextflow": "24.01.0"
++            "nextflow": "23.10.1"
+         },
+-        "timestamp": "2024-02-28T15:25:52.837288"
++        "timestamp": "2024-05-17T22:01:43.486256"
++    },
++    "Single-end-stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test_single_end",
++                            "single_end": true
++                        },
++                        "test_single_end.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "1": [
++                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
++                ],
++                "reads": [
++                    [
++                        {
++                            "id": "test_single_end",
++                            "single_end": true
++                        },
++                        "test_single_end.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-17T21:40:42.335786"
+     },
+     "Paired-end": {
+         "content": [
+@@ -71,8 +106,49 @@
+         ],
+         "meta": {
+             "nf-test": "0.8.4",
+-            "nextflow": "24.01.0"
++            "nextflow": "23.10.1"
+         },
+-        "timestamp": "2024-02-28T15:26:42.466223"
++        "timestamp": "2024-05-17T22:09:55.6396"
++    },
++    "Paired-end-stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test_paired_end",
++                            "single_end": false
++                        },
++                        [
++                            "test_paired_end_1.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_paired_end_2.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "1": [
++                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
++                ],
++                "reads": [
++                    [
++                        {
++                            "id": "test_paired_end",
++                            "single_end": false
++                        },
++                        [
++                            "test_paired_end_1.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
++                            "test_paired_end_2.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-17T20:35:44.782058"
+     }
+ }
+--- modules/nf-core/sratools/fasterqdump/tests/main.nf.test
++++ modules/nf-core/sratools/fasterqdump/tests/main.nf.test
+@@ -1,7 +1,6 @@
+ nextflow_process {
+     name "Test Process SRATOOLS_FASTERQDUMP"
+     script "../main.nf"
+-    config "./nextflow.config"
+     process "SRATOOLS_FASTERQDUMP"
+     tag "modules"
+     tag "modules_nfcore"
+@@ -70,4 +69,54 @@
+             )
+         }
+     }
++
++    test("Single-end-stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                input[0] = [
++                    [ id:'test_single_end', single_end:true ],
++                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR13255544.tar.gz', checkIfExists: true)
++                ]
++                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
++                input[2] = []
++                """
++            }
++        }
++
++        then {
++            assertAll(
++            { assert process.success },
++            { assert snapshot(process.out).match() }
++            )
++        }
++    }
++
++    test("Paired-end-stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                input[0] = [
++                    [ id:'test_paired_end', single_end:false ],
++                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR11140744.tar.gz', checkIfExists: true)
++                ]
++                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
++                input[2] = []
++                """
++            }
++        }
++
++        then {
++            assertAll(
++            { assert process.success },
++            { assert snapshot(process.out).match() }
++            )
++        }
++    }
+ }
+
+--- modules/nf-core/sratools/fasterqdump/tests/nextflow.config
++++ /dev/null
+@@ -1,5 +0,0 @@
+-process {
+-    withName: SRATOOLS_FASTERQDUMP {
+-        ext.args = ''
+-    }
+-}
+************************************************************
diff --git a/modules/nf-core/sratools/fasterqdump/tests/main.nf.test b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test
index cb81496f..6996cd18 100644
--- a/modules/nf-core/sratools/fasterqdump/tests/main.nf.test
+++ b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test
@@ -1,7 +1,6 @@
 nextflow_process {
     name "Test Process SRATOOLS_FASTERQDUMP"
     script "../main.nf"
-    config "./nextflow.config"
     process "SRATOOLS_FASTERQDUMP"
     tag "modules"
     tag "modules_nfcore"
@@ -70,4 +69,54 @@ nextflow_process {
             )
         }
     }
+
+    test("Single-end-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test_single_end', single_end:true ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR13255544.tar.gz', checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("Paired-end-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test_paired_end', single_end:false ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR11140744.tar.gz', checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
 }
diff --git a/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
index 14a6429d..5d0c3e7c 100644
--- a/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
+++ b/modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
@@ -30,9 +30,44 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-28T15:25:52.837288"
+        "timestamp": "2024-05-17T22:01:43.486256"
+    },
+    "Single-end-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_single_end",
+                            "single_end": true
+                        },
+                        "test_single_end.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test_single_end",
+                            "single_end": true
+                        },
+                        "test_single_end.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-17T21:40:42.335786"
     },
     "Paired-end": {
         "content": [
@@ -71,8 +106,49 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-17T22:09:55.6396"
+    },
+    "Paired-end-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_paired_end",
+                            "single_end": false
+                        },
+                        [
+                            "test_paired_end_1.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_paired_end_2.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test_paired_end",
+                            "single_end": false
+                        },
+                        [
+                            "test_paired_end_1.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_paired_end_2.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-28T15:26:42.466223"
+        "timestamp": "2024-05-17T20:35:44.782058"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/sratools/fasterqdump/tests/nextflow.config b/modules/nf-core/sratools/fasterqdump/tests/nextflow.config
deleted file mode 100644
index 23e4100b..00000000
--- a/modules/nf-core/sratools/fasterqdump/tests/nextflow.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: SRATOOLS_FASTERQDUMP {
-        ext.args = ''
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/sratools/prefetch/main.nf b/modules/nf-core/sratools/prefetch/main.nf
index 170f1753..46e7cb3f 100644
--- a/modules/nf-core/sratools/prefetch/main.nf
+++ b/modules/nf-core/sratools/prefetch/main.nf
@@ -32,4 +32,16 @@ process SRATOOLS_PREFETCH {
     }
 
     template 'retry_with_backoff.sh'
+
+    stub:
+    """
+    mkdir $id
+    touch $id/${id}.sra
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sratools: \$(prefetch --version 2>&1 | grep -Eo '[0-9.]+')
+        curl: \$(curl --version | head -n 1 | sed 's/^curl //; s/ .*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/sratools/prefetch/meta.yml b/modules/nf-core/sratools/prefetch/meta.yml
index ff54229f..7ed42d49 100644
--- a/modules/nf-core/sratools/prefetch/meta.yml
+++ b/modules/nf-core/sratools/prefetch/meta.yml
@@ -54,3 +54,4 @@ authors:
   - "@Midnighter"
 maintainers:
   - "@Midnighter"
+  - "@gallvp"
diff --git a/modules/nf-core/sratools/prefetch/sratools-prefetch.diff b/modules/nf-core/sratools/prefetch/sratools-prefetch.diff
new file mode 100644
index 00000000..25a938e7
--- /dev/null
+++ b/modules/nf-core/sratools/prefetch/sratools-prefetch.diff
@@ -0,0 +1,112 @@
+Changes in module 'nf-core/sratools/prefetch'
+--- modules/nf-core/sratools/prefetch/meta.yml
++++ modules/nf-core/sratools/prefetch/meta.yml
+@@ -54,3 +54,4 @@
+   - "@Midnighter"
+ maintainers:
+   - "@Midnighter"
++  - "@gallvp"
+
+--- modules/nf-core/sratools/prefetch/main.nf
++++ modules/nf-core/sratools/prefetch/main.nf
+@@ -32,4 +32,16 @@
+     }
+ 
+     template 'retry_with_backoff.sh'
++
++    stub:
++    """
++    mkdir $id
++    touch $id/${id}.sra
++
++    cat <<-END_VERSIONS > versions.yml
++    "${task.process}":
++        sratools: \$(prefetch --version 2>&1 | grep -Eo '[0-9.]+')
++        curl: \$(curl --version | head -n 1 | sed 's/^curl //; s/ .*\$//')
++    END_VERSIONS
++    """
+ }
+
+--- modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
++++ modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
+@@ -1,4 +1,43 @@
+ {
++    "sratools/prefetch/stub": {
++        "content": [
++            {
++                "0": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            "DRR000774.sra:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "1": [
++                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
++                ],
++                "sra": [
++                    [
++                        {
++                            "id": "test",
++                            "single_end": false
++                        },
++                        [
++                            "DRR000774.sra:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        ]
++                    ]
++                ],
++                "versions": [
++                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
++                ]
++            }
++        ],
++        "meta": {
++            "nf-test": "0.8.4",
++            "nextflow": "23.10.1"
++        },
++        "timestamp": "2024-05-17T20:07:31.627115"
++    },
+     "sratools/prefetch with sralite": {
+         "content": [
+             {
+@@ -77,4 +116,4 @@
+         },
+         "timestamp": "2024-02-28T11:48:37.428307"
+     }
+-}
++}
+--- modules/nf-core/sratools/prefetch/tests/main.nf.test
++++ modules/nf-core/sratools/prefetch/tests/main.nf.test
+@@ -46,4 +46,26 @@
+             )
+         }
+     }
++
++    test("sratools/prefetch/stub") {
++
++        options '-stub'
++
++        when {
++            process {
++                """
++                input[0] = Channel.of([ [ id:'test', single_end:false ], 'DRR000774' ])
++                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
++                input[2] = []
++                """
++            }
++        }
++
++        then {
++            assertAll (
++            { assert process.success },
++            { assert snapshot(process.out).match() }
++            )
++        }
++    }
+ }
+
+************************************************************
diff --git a/modules/nf-core/sratools/prefetch/tests/main.nf.test b/modules/nf-core/sratools/prefetch/tests/main.nf.test
index f59de7cb..92034d40 100644
--- a/modules/nf-core/sratools/prefetch/tests/main.nf.test
+++ b/modules/nf-core/sratools/prefetch/tests/main.nf.test
@@ -46,4 +46,26 @@ nextflow_process {
             )
         }
     }
+
+    test("sratools/prefetch/stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([ [ id:'test', single_end:false ], 'DRR000774' ])
+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
 }
diff --git a/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap b/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
index 67795ebb..82a1969c 100644
--- a/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
+++ b/modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
@@ -1,4 +1,43 @@
 {
+    "sratools/prefetch/stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "DRR000774.sra:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
+                ],
+                "sra": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "DRR000774.sra:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-17T20:07:31.627115"
+    },
     "sratools/prefetch with sralite": {
         "content": [
             {
@@ -77,4 +116,4 @@
         },
         "timestamp": "2024-02-28T11:48:37.428307"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/stub/params.json b/tests/stub/params.json
index 4f11914c..d3ea8333 100644
--- a/tests/stub/params.json
+++ b/tests/stub/params.json
@@ -20,7 +20,7 @@
     "hic_skip_fastqc": false,
     "synteny_skip": false,
     "synteny_xref_assemblies": "assets/xrefsheet.csv",
-    "merqury_skip": true,
+    "merqury_skip": false,
     "max_cpus": 2,
     "max_memory": "6.GB",
     "max_time": "6.h"

From e4bbd3187da88dd384639fed65ed630fdffde18b Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Sat, 18 May 2024 21:12:42 +1200
Subject: [PATCH 44/76] Nowing using a single FETCHNGS workflow to download sra

---
 conf/modules.config                           |   2 -
 .../utils_nfcore_assemblyqc_pipeline/main.nf  |  10 +-
 workflows/assemblyqc.nf                       | 143 +++++++++---------
 3 files changed, 79 insertions(+), 76 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 7910d0cc..a5fd9ad0 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -142,8 +142,6 @@ process {
         ]
     }
 
-
-
     withName: FILTER_BY_LENGTH {
         ext.args = params.tidk_filter_by_size ? "-m ${params.tidk_filter_size_bp}" : ''
         ext.prefix = { "${meta.id}.filtered" }
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 489b1c36..64118e4b 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -103,8 +103,8 @@ workflow PIPELINE_INITIALISATION {
                                             )
                                             | map { sample, fq ->
                                                 "$fq" != 'is_sra'
-                                                ? [ [ id: sample, single_end: false, is_sra: false ], fq ]
-                                                : [ [ id: sample, single_end: false, is_sra: true ], sample ]
+                                                ? [ [ id: sample, single_end: false, is_sra: false, type: 'hic' ], fq ]
+                                                : [ [ id: sample, single_end: false, is_sra: true, type: 'hic' ], sample ]
                                             }
 
     ch_xref_assembly                        = params.synteny_skip || ! params.synteny_xref_assemblies
@@ -342,14 +342,14 @@ def validateAndNormaliseReadsTupe ( fid, metas, reads ) {
 
     if ( metas.first().is_sra ) { // SRA
         return [
-            [ id:individualID, single_end:false, is_sra:true, assemblies:tags ],
+            [ id:individualID, single_end:false, is_sra:true, type: 'reads', assemblies:tags ],
             reads.first()
         ]
     }
 
     if ( endedness.unique().first() ) { // Single ended
         return [
-            [ id:individualID, single_end:true, is_sra:false, assemblies:tags ],
+            [ id:individualID, single_end:true, is_sra:false, type: 'reads', assemblies:tags ],
             reads.first().collect { file(it, checkIfExists: true) }
         ]
     }
@@ -361,7 +361,7 @@ def validateAndNormaliseReadsTupe ( fid, metas, reads ) {
     }
 
     return [
-        [ id:individualID, single_end:false, is_sra:false, assemblies:tags ],
+        [ id:individualID, single_end:false, is_sra:false, type: 'reads', assemblies:tags ],
         reads.first().collect { file(it, checkIfExists: true) }
     ]
 }
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index ea2729d7..dc9b8990 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -25,8 +25,7 @@ include { FASTK_FASTK                       } from '../modules/nf-core/fastk/fas
 include { MERQURYFK_MERQURYFK               } from '../modules/nf-core/merquryfk/merquryfk/main'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
-include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_HIC      } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
-include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_READS    } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
+include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as FETCHNGS  } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -256,6 +255,75 @@ workflow ASSEMBLYQC {
                                                 [ tag, fa ]
                                             }
 
+    // MODULE: CAT_CAT as TAG_ASSEMBLY
+    TAG_ASSEMBLY (
+        ch_clean_assembly.map { tag, fa -> [ [ id: tag ], fa ] }
+    )
+
+    ch_clean_assembly_tagged                = TAG_ASSEMBLY.out.file_out
+    ch_versions                             = ch_versions.mix(TAG_ASSEMBLY.out.versions)
+
+    // Prepare channels for FETCHNGS
+    // HiC
+    ch_hic_input_assembly                   = ! params.hic
+                                            ? Channel.empty()
+                                            : ch_clean_assembly
+                                            | map { tag, fa -> [ [ id: tag ], fa ] }
+
+    ch_hic_reads_branch                     = ch_hic_reads
+                                            | combine(ch_hic_input_assembly.first()) // Wait till first clean assembly arrives
+                                            | map { meta, fq, meta2, fasta -> [ meta, fq ] }
+                                            | branch { meta, fq ->
+                                                sra: meta.is_sra
+                                                rest: ! meta.is_sra
+                                            }
+    // Reads
+    ch_reads_assemblies                     = ch_reads
+                                            | combine(
+                                                ch_clean_assembly_tagged
+                                                | map { [ it ] }
+                                                | collect
+                                                | map { [ it ] }
+                                            )
+                                            | map { meta, fq, assemblies ->
+                                                [
+                                                    [
+                                                        id: meta.id,
+                                                        single_end: meta.single_end,
+                                                        is_sra: meta.is_sra,
+                                                        type: meta.type
+                                                    ],
+                                                    fq,
+                                                    assemblies
+                                                        .findAll { meta2, fasta -> meta2.id in meta.assemblies }
+                                                        .collect { meta2, fasta -> fasta }
+                                                        .flatten()
+                                                        .sort(false)
+                                                ]
+                                            }
+
+    ch_reads_branch                         = ch_reads_assemblies
+                                            | map { meta, fq, fastas -> [ meta, fq ] }
+                                            | branch { meta, fq ->
+                                                sra: meta.is_sra
+                                                rest: ! meta.is_sra
+                                            }
+
+    // MODULE: FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as FETCHNGS
+    ch_fetchngs_inputs                      = ch_hic_reads_branch.sra
+                                            | mix(ch_reads_branch.sra)
+    FETCHNGS(
+        ch_fetchngs_inputs,
+        []
+    )
+
+    ch_fetchngs                             = FETCHNGS.out.reads
+                                            | branch { meta, fq ->
+                                                hic: meta.type == 'hic'
+                                                reads: meta.type == 'reads'
+                                            }
+    ch_versions                             = ch_versions.mix(FETCHNGS.out.versions)
+
     // MODULE: ASSEMBLATHON_STATS
     ASSEMBLATHON_STATS(
         ch_clean_assembly,
@@ -378,29 +446,9 @@ workflow ASSEMBLYQC {
     ch_kraken2_plot                         = FASTA_KRAKEN2.out.plot
     ch_versions                             = ch_versions.mix(FASTA_KRAKEN2.out.versions)
 
-
-    // MODULE: FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_HIC
-    ch_hic_reads_branch                    = ch_hic_reads
-                                            | branch { meta, fq ->
-                                                sra: meta.is_sra
-                                                rest: ! meta.is_sra
-                                            }
-
-    DOWNLOAD_HIC(
-        ch_hic_reads_branch.sra,
-        []
-    )
-
-    ch_versions                             = ch_versions.mix(DOWNLOAD_HIC.out.versions)
-    ch_hic_read_files                       = DOWNLOAD_HIC.out.reads
-                                            | mix(ch_hic_reads_branch.rest)
-
     // SUBWORKFLOW: FQ2HIC
-    ch_hic_input_assembly                   = ! params.hic
-                                            ? Channel.empty()
-                                            : ch_clean_assembly
-                                            | map { tag, fa -> [ [ id: tag ], fa ] }
-
+    ch_hic_read_files                       = ch_fetchngs.hic
+                                            | mix(ch_hic_reads_branch.rest)
     FQ2HIC(
         ch_hic_read_files,
         ch_hic_input_assembly,
@@ -428,52 +476,9 @@ workflow ASSEMBLYQC {
     ch_synteny_plots                        = FASTA_SYNTENY.out.png.mix(FASTA_SYNTENY.out.html)
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
-    // MODULE: CAT_CAT as TAG_ASSEMBLY
-    TAG_ASSEMBLY (
-        ch_clean_assembly.map { tag, fa -> [ [ id: tag ], fa ] }
-    )
-
-    ch_clean_assembly_tagged                = TAG_ASSEMBLY.out.file_out
-    ch_versions                             = ch_versions.mix(TAG_ASSEMBLY.out.versions)
-
-    // MODULE: FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as DOWNLOAD_READS
-    ch_reads_assemblies                     = ch_reads
-                                            | combine(
-                                                ch_clean_assembly_tagged
-                                                | map { [ it ] }
-                                                | collect
-                                                | map { [ it ] }
-                                            )
-                                            | map { meta, fq, assemblies ->
-                                                [
-                                                    [ id: meta.id, single_end: meta.single_end, is_sra: meta.is_sra ],
-                                                    fq,
-                                                    assemblies
-                                                        .findAll { meta2, fasta -> meta2.id in meta.assemblies }
-                                                        .collect { meta2, fasta -> fasta }
-                                                        .flatten()
-                                                        .sort(false)
-                                                ]
-                                            }
-
-    ch_reads_branch                         = ch_reads_assemblies
-                                            | map { meta, fq, fastas -> [ meta, fq ] }
-                                            | branch { meta, fq ->
-                                                sra: meta.is_sra
-                                                rest: ! meta.is_sra
-                                            }
-
-    DOWNLOAD_READS(
-        ch_reads_branch.sra,
-        []
-    )
-
-    ch_reads_files                          = DOWNLOAD_READS.out.reads
-                                            | mix(ch_reads_branch.rest)
-
-    ch_versions                             = ch_versions.mix(DOWNLOAD_READS.out.versions)
-
     // MODULE: FASTK_FASTK
+    ch_reads_files                          = ch_fetchngs.reads
+                                            | mix(ch_reads_branch.rest)
     FASTK_FASTK ( ch_reads_files )
 
     ch_reads_fastk_hist                     = FASTK_FASTK.out.hist

From 94e80c9dd3928c5a94e4e3380f69c01ddd042cf7 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Sun, 19 May 2024 21:23:17 +1200
Subject: [PATCH 45/76] Added phased2x example for merqury.fk analysis

---
 assets/schema_input.json                    | 31 ++++++---------------
 docs/usage.md                               |  3 +-
 tests/merqury.fk/phased2x/assemblysheet.csv |  3 ++
 tests/merqury.fk/phased2x/params.json       | 10 +++++++
 4 files changed, 23 insertions(+), 24 deletions(-)
 create mode 100644 tests/merqury.fk/phased2x/assemblysheet.csv
 create mode 100644 tests/merqury.fk/phased2x/params.json

diff --git a/assets/schema_input.json b/assets/schema_input.json
index f415c25e..7c58c538 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -75,36 +75,21 @@
                 ],
                 "dependentRequired": ["reads_1"]
             },
-            "maternal_reads_1": {
-                "errorMessage": "maternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+            "is_maternal": {
                 "anyOf": [
                     {
                         "type": "string",
-                        "pattern": "^SR\\w+$|^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
-                    },
-                    {
-                        "type": "string",
-                        "maxLength": 0
-                    }
-                ],
-                "dependentRequired": ["reads_1", "paternal_reads_1"]
-            },
-            "maternal_reads_2": {
-                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
-                "anyOf": [
-                    {
-                        "type": "string",
-                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                        "pattern": "^(yes|no)$"
                     },
                     {
                         "type": "string",
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["maternal_reads_1"]
+                "errorMessage": "Maternal haplotype should be indicated by 'yes' or 'no'"
             },
-            "paternal_reads_1": {
-                "errorMessage": "paternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+            "parent_reads_1": {
+                "errorMessage": "parent_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
@@ -115,9 +100,9 @@
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["reads_1", "maternal_reads_1"]
+                "dependentRequired": ["reads_1", "is_maternal"]
             },
-            "paternal_reads_2": {
+            "parent_reads_2": {
                 "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
@@ -129,7 +114,7 @@
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["paternal_reads_1"]
+                "dependentRequired": ["parent_reads_1"]
             }
         },
         "required": ["tag", "fasta"]
diff --git a/docs/usage.md b/docs/usage.md
index 9fc62733..50630160 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -10,12 +10,13 @@ You will need to create an assemblysheet with information about the assemblies y
 - `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
 - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) when performing synteny analysis
 - `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY.FK.
-- `reads_2 [Optional]`: If `reads_1` is a SRA ID, this column is ignored. Otherwise, this column should list the second file of the paired reads.
+- `reads_2 [Optional]`: This column lists the second file if paired reads are used. If `reads_1` is a SRA ID, this column is ignored.
 
 See following assemblysheet examples for MERQURY.FK analysis.
 
 - [assemblysheet - 1x](../assets/assemblysheetv2.csv)
 - [assemblysheet - mixed2x](../tests/merqury.fk/mixed2x/assemblysheet.csv)
+- [assemblysheet - phased2x](../tests/merqury.fk/phased2x/assemblysheet.csv)
 
 ## External databases
 
diff --git a/tests/merqury.fk/phased2x/assemblysheet.csv b/tests/merqury.fk/phased2x/assemblysheet.csv
new file mode 100644
index 00000000..852fd392
--- /dev/null
+++ b/tests/merqury.fk/phased2x/assemblysheet.csv
@@ -0,0 +1,3 @@
+tag,fasta,reads_1
+COL,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fasta,SRR3703081
+CVI,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fasta,SRR3703081
diff --git a/tests/merqury.fk/phased2x/params.json b/tests/merqury.fk/phased2x/params.json
new file mode 100644
index 00000000..697703b9
--- /dev/null
+++ b/tests/merqury.fk/phased2x/params.json
@@ -0,0 +1,10 @@
+{
+    "config_profile_name": "Test profile",
+    "config_profile_description": "Merqury.FK test for a phased diploid assembly contained in separate fasta",
+    "input": "tests/merqury.fk/phased2x/assemblysheet.csv",
+    "merqury_skip": false,
+    "merqury_kmer_length": 21,
+    "max_cpus": 8,
+    "max_memory": "32.GB",
+    "max_time": "6.h"
+}

From 8a7eb131b84bc96150c7c16b88f7f76841b2144d Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 21 May 2024 13:59:44 +1200
Subject: [PATCH 46/76] Enroute to MERQURY.FK trio mode

---
 assets/schema_input.json                      |  31 ++-
 conf/modules.config                           |   1 +
 docs/usage.md                                 |  26 +-
 main.nf                                       |   6 +
 modules/local/merquryfk_hapmaker.nf           | 101 +++++++
 modules/nf-core/merquryfk/merquryfk/main.nf   |  18 +-
 .../utils_nfcore_assemblyqc_pipeline/main.nf  |  58 +++-
 .../merqury.fk/phased2x.mp/assemblysheet.csv  |   4 +
 .../phased2x.mp/assemblysheet.csv.bk          |   3 +
 tests/merqury.fk/phased2x.mp/params.json      |  10 +
 workflows/assemblyqc.nf                       | 249 +++++++++++++++---
 11 files changed, 433 insertions(+), 74 deletions(-)
 create mode 100644 modules/local/merquryfk_hapmaker.nf
 create mode 100644 tests/merqury.fk/phased2x.mp/assemblysheet.csv
 create mode 100644 tests/merqury.fk/phased2x.mp/assemblysheet.csv.bk
 create mode 100644 tests/merqury.fk/phased2x.mp/params.json

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 7c58c538..f415c25e 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -75,21 +75,36 @@
                 ],
                 "dependentRequired": ["reads_1"]
             },
-            "is_maternal": {
+            "maternal_reads_1": {
+                "errorMessage": "maternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
-                        "pattern": "^(yes|no)$"
+                        "pattern": "^SR\\w+$|^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ],
+                "dependentRequired": ["reads_1", "paternal_reads_1"]
+            },
+            "maternal_reads_2": {
+                "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(a|asta|as|sa|na|astq|q)(\\.gz)?$"
                     },
                     {
                         "type": "string",
                         "maxLength": 0
                     }
                 ],
-                "errorMessage": "Maternal haplotype should be indicated by 'yes' or 'no'"
+                "dependentRequired": ["maternal_reads_1"]
             },
-            "parent_reads_1": {
-                "errorMessage": "parent_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
+            "paternal_reads_1": {
+                "errorMessage": "paternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
                         "type": "string",
@@ -100,9 +115,9 @@
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["reads_1", "is_maternal"]
+                "dependentRequired": ["reads_1", "maternal_reads_1"]
             },
-            "parent_reads_2": {
+            "paternal_reads_2": {
                 "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'",
                 "anyOf": [
                     {
@@ -114,7 +129,7 @@
                         "maxLength": 0
                     }
                 ],
-                "dependentRequired": ["parent_reads_1"]
+                "dependentRequired": ["paternal_reads_1"]
             }
         },
         "required": ["tag", "fasta"]
diff --git a/conf/modules.config b/conf/modules.config
index a5fd9ad0..04f77eca 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -331,6 +331,7 @@ process {
     }
 
     withName: MERQURYFK_MERQURYFK {
+        ext.args = '-v'
         publishDir = [
             [
                 path: { "${params.outdir}/merquryfk/${meta.id}" },
diff --git a/docs/usage.md b/docs/usage.md
index 50630160..f262522f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -2,21 +2,15 @@
 
 ## Assemblysheet input
 
-You will need to create an assemblysheet with information about the assemblies you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 5 columns, and a header row. An [example assemblysheet](../assets/assemblysheetv2.csv) has been provided with the pipeline. Its fields are:
+You will need to create an assemblysheet with information about the assemblies you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with at least two columns, and a header row. An [example assemblysheet](../assets/assemblysheet.csv) has been provided with the pipeline. Its fields are:
 
 - `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report
 - `fasta:` FASTA file
 - `gff3 [Optional]:` GFF3 annotation file if available
 - `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
 - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) when performing synteny analysis
-- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY.FK.
-- `reads_2 [Optional]`: This column lists the second file if paired reads are used. If `reads_1` is a SRA ID, this column is ignored.
-
-See following assemblysheet examples for MERQURY.FK analysis.
 
-- [assemblysheet - 1x](../assets/assemblysheetv2.csv)
-- [assemblysheet - mixed2x](../tests/merqury.fk/mixed2x/assemblysheet.csv)
-- [assemblysheet - phased2x](../tests/merqury.fk/phased2x/assemblysheet.csv)
+See the [Merqury.FK](#merquryfk-k-mer-analysis) section For description of assemblysheet columns related to k-mer analysis with Merqury.FK.
 
 ## External databases
 
@@ -72,7 +66,7 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
 
 ### HiC
 
-- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq|fq).gz'
+- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq|fq).gz'. These reads are applied to each assembly listed by assemblysheet.
 - `hic_skip_fastp`: Skip fastp trimming
 - `hic_skip_fastqc`: Skip QC by fastqc
 - `hic_fastp_ext_args`: Additional arguments for fastp (default: '--qualified_quality_phred 20 --length_required 50')
@@ -93,6 +87,20 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
 
 ### Merqury.FK K-mer analysis
 
+#### Additional assemblysheet columns
+
+- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY.FK. A genome can only have one or two haplotypes.
+- `reads_2 [Optional]`: This column lists the second file if paired reads are used. If `reads_1` is a SRA ID, this column is ignored.
+
+See following assemblysheet examples for MERQURY.FK analysis.
+
+- [assemblysheet - 1x](../assets/assemblysheetv2.csv)
+- [assemblysheet - mixed2x](../tests/merqury.fk/mixed2x/assemblysheet.csv)
+- [assemblysheet - phased2x](../tests/merqury.fk/phased2x/assemblysheet.csv)
+- [assemblysheet - phased2x with parent reads](../tests/merqury.fk/phased2x.mp/assemblysheet.csv)
+
+#### Parameters
+
 - `merqury_kmer_length`: kmer length for merqury analysis
 
 ## Running the pipeline
diff --git a/main.nf b/main.nf
index 04eef6ad..91e82dff 100755
--- a/main.nf
+++ b/main.nf
@@ -35,6 +35,8 @@ workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
     ch_hic_reads
     ch_xref_assembly
     ch_reads
+    ch_maternal_reads
+    ch_paternal_reads
     ch_params_as_json
     ch_summary_params_as_json
 
@@ -47,6 +49,8 @@ workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC {
         ch_hic_reads,
         ch_xref_assembly,
         ch_reads,
+        ch_maternal_reads,
+        ch_paternal_reads,
         ch_params_as_json,
         ch_summary_params_as_json
     )
@@ -83,6 +87,8 @@ workflow {
         PIPELINE_INITIALISATION.out.hic_reads,
         PIPELINE_INITIALISATION.out.xref_assembly,
         PIPELINE_INITIALISATION.out.reads,
+        PIPELINE_INITIALISATION.out.maternal_reads,
+        PIPELINE_INITIALISATION.out.paternal_reads,
         PIPELINE_INITIALISATION.out.params_as_json,
         PIPELINE_INITIALISATION.out.summary_params_as_json
     )
diff --git a/modules/local/merquryfk_hapmaker.nf b/modules/local/merquryfk_hapmaker.nf
new file mode 100644
index 00000000..311a3b79
--- /dev/null
+++ b/modules/local/merquryfk_hapmaker.nf
@@ -0,0 +1,101 @@
+process MERQURYFK_HAPMAKER {
+    tag "$meta.id"
+    label 'process_medium'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.2'
+
+    input:
+    tuple val(meta), path(ktab)
+    path(mktab)
+    path(pktab)
+
+    output:
+    tuple val(meta), path('*_mat.hap.ktab*', hidden: true)  , emit: mat_hapmers
+    tuple val(meta), path('*_pat.hap.ktab*', hidden: true)  , emit: pat_hapmers
+    path "versions.yml"                                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "MERQURYFK_HAPMAKER module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args            = task.ext.args ?: ''
+    def prefix          = task.ext.prefix ?: "${meta.id}"
+    def mktab_file      = mktab.find { it.toString().endsWith(".ktab") }
+    def pktab_file      = pktab.find { it.toString().endsWith(".ktab") }
+    def ktab_file       = ktab.find  { it.toString().endsWith(".ktab") }
+    def mktab_basename  = mktab_file.baseName
+    def pktab_basename  = pktab_file.baseName
+    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    HAPmaker \\
+        $args \\
+        -T$task.cpus \\
+        $mktab_file \\
+        $pktab_file \\
+        $ktab_file
+
+    mv ${mktab_basename}.hap.ktab ${prefix}_mat.hap.ktab
+
+    find . -name ".${mktab_basename}.hap.*" \\
+    | while read -r file; do
+        new_name=\$(echo "\$file" | sed "s/${mktab_basename}/${prefix}_mat/")
+        mv "\$file" "\$new_name"
+    done
+
+    mv ${pktab_basename}.hap.ktab ${prefix}_pat.hap.ktab
+
+    find . -name ".${pktab_basename}.hap.*" \\
+    | while read -r file; do
+        new_name=\$(echo "\$file" | sed "s/${pktab_basename}/${prefix}_pat/")
+        mv "\$file" "\$new_name"
+    done
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+        merquryfk: $MERQURY_VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "MERQURYFK_HAPMAKER module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args            = task.ext.args ?: ''
+    def prefix          = task.ext.prefix ?: "${meta.id}"
+    def mktab_file      = mktab.find { it.toString().endsWith(".ktab") }
+    def pktab_file      = pktab.find { it.toString().endsWith(".ktab") }
+    def ktab_file       = ktab.find  { it.toString().endsWith(".ktab") }
+    def mktab_basename  = mktab_file.baseName
+    def pktab_basename  = pktab_file.baseName
+    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    echo \\
+    "HAPmaker \\
+        $args \\
+        -T$task.cpus \\
+        $mktab_file \\
+        $pktab_file \\
+        $ktab_file"
+
+    touch ${prefix}_mat.hap.ktab
+    touch .${prefix}_mat.hap.ktab.1
+
+    touch ${prefix}_pat.hap.ktab
+    touch .${prefix}_pat.hap.ktab.1
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+        merquryfk: $MERQURY_VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/merquryfk/merquryfk/main.nf b/modules/nf-core/merquryfk/merquryfk/main.nf
index f22e4431..ecc0f70f 100644
--- a/modules/nf-core/merquryfk/merquryfk/main.nf
+++ b/modules/nf-core/merquryfk/merquryfk/main.nf
@@ -35,15 +35,16 @@ process MERQURYFK_MERQURYFK {
     if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
         error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
     }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def args            = task.ext.args         ?: ''
+    prefix              = task.ext.prefix       ?: "${meta.id}"
+    def ktabs           = fastk_ktab.findAll    { it.toString().endsWith(".ktab") }.collect { "$it" }.join(' ')
+    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     MerquryFK \\
         $args \\
         -T$task.cpus \\
-        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }} \\
+        $ktabs \\
         $assembly \\
         $haplotigs \\
         $prefix
@@ -61,9 +62,10 @@ process MERQURYFK_MERQURYFK {
     if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
         error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
     }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def args            = task.ext.args         ?: ''
+    prefix              = task.ext.prefix       ?: "${meta.id}"
+    def ktabs           = fastk_ktab.findAll    { it.toString().endsWith(".ktab") }.collect { "$it" }.join(' ')
+    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
 
     def touch_asm_bed   = "touch ${prefix}.${assembly.baseName}_only.bed"
@@ -84,7 +86,7 @@ process MERQURYFK_MERQURYFK {
     "MerquryFK \\
         $args \\
         -T$task.cpus \\
-        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }} \\
+        $ktabs \\
         $assembly \\
         $haplotigs \\
         $prefix"
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 64118e4b..0bc0efd6 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -135,7 +135,41 @@ workflow PIPELINE_INITIALISATION {
                                             }
                                             | groupTuple
                                             | map { fid, metas, reads ->
-                                                validateAndNormaliseReadsTupe ( fid, metas, reads )
+                                                validateAndNormaliseReadsTuple ( fid, metas, reads, 'reads' )
+                                            }
+
+    ch_maternal_reads                       = params.merqury_skip
+                                            ? Channel.empty()
+                                            : ch_input_validated
+                                            | map { input_data ->
+                                                def tag                 = input_data[0]
+                                                def maternal_reads_1    = input_data[7]
+                                                def maternal_reads_2    = input_data[8]
+
+                                                maternal_reads_1
+                                                ? extractReadsTuple ( tag, maternal_reads_1, maternal_reads_2 )
+                                                : null
+                                            }
+                                            | groupTuple
+                                            | map { fid, metas, m_reads ->
+                                                validateAndNormaliseReadsTuple ( fid, metas, m_reads, 'maternal' )
+                                            }
+
+    ch_paternal_reads                       = params.merqury_skip
+                                            ? Channel.empty()
+                                            : ch_input_validated
+                                            | map { input_data ->
+                                                def tag                 = input_data[0]
+                                                def paternal_reads_1    = input_data[9]
+                                                def paternal_reads_2    = input_data[10]
+
+                                                paternal_reads_1
+                                                ? extractReadsTuple ( tag, paternal_reads_1, paternal_reads_2 )
+                                                : null
+                                            }
+                                            | groupTuple
+                                            | map { fid, metas, m_reads ->
+                                                validateAndNormaliseReadsTuple ( fid, metas, m_reads, 'paternal' )
                                             }
 
     // Initialise parameter channels
@@ -147,6 +181,8 @@ workflow PIPELINE_INITIALISATION {
     hic_reads                               = ch_hic_reads
     xref_assembly                           = ch_xref_assembly_validated
     reads                                   = ch_reads
+    maternal_reads                          = ch_maternal_reads
+    paternal_reads                          = ch_paternal_reads
     params_as_json                          = ch_params_as_json
     summary_params_as_json                  = ch_summary_params_as_json
     versions                                = ch_versions
@@ -324,32 +360,34 @@ def extractReadsTuple(tag, reads_1, reads_2) {
     ]
 }
 
-def validateAndNormaliseReadsTupe ( fid, metas, reads ) {
+def validateAndNormaliseReadsTuple ( fid, metas, reads, readsType ) {
 
     def tags        = metas.collect { it.id }.flatten()
     def endedness   = metas.collect { it.single_end }.flatten()
+    def identifier  = readsType == 'reads' ? '' : "${readsType}_"
 
     // Validate
     if ( endedness.unique().size() != 1 ) {
-        error("Please check input assemblysheet -> Following assemblies have different reads_1 and reads_2: ${tags}")
+        error("Please check input assemblysheet -> Following assemblies have different ${identifier}reads_1 and ${identifier}reads_2: ${tags}")
     }
 
-    if ( tags.size() > 2 ) {
-        error("Please check input assemblysheet -> More than two assemblies (${tags}) are in the same read group: ${fid}")
+    if ( readsType == 'reads' && tags.size() > 2 ) {
+        error("Please check input assemblysheet -> More than two assemblies (${tags}) are in the same genome group defined by ${identifier}reads_1: ${fid.fid}")
     }
 
-    def individualID = tags.join('-and-') + "-reads-" + fid.fid.replaceAll(/\./, '_')
+    def groupIDPrefix = readsType == 'reads' ? ( tags.join('-and-') + "-${readsType}-" ) : ''
+    def groupID = groupIDPrefix + fid.fid.replaceAll(/\./, '_')
 
     if ( metas.first().is_sra ) { // SRA
         return [
-            [ id:individualID, single_end:false, is_sra:true, type: 'reads', assemblies:tags ],
+            [ id:groupID, single_end:false, is_sra:true, type: readsType, assemblies:tags ],
             reads.first()
         ]
     }
 
     if ( endedness.unique().first() ) { // Single ended
         return [
-            [ id:individualID, single_end:true, is_sra:false, type: 'reads', assemblies:tags ],
+            [ id:groupID, single_end:true, is_sra:false, type: readsType, assemblies:tags ],
             reads.first().collect { file(it, checkIfExists: true) }
         ]
     }
@@ -357,11 +395,11 @@ def validateAndNormaliseReadsTupe ( fid, metas, reads ) {
     def reads_2 = reads.collect { it[1] }
 
     if ( reads_2.unique().size() != 1 ) {
-        error("Please check input assemblysheet -> Following assemblies have different reads_1 and reads_2: ${tags}")
+        error("Please check input assemblysheet -> Following assemblies have different ${identifier}reads_1 and ${identifier}reads_2: ${tags}")
     }
 
     return [
-        [ id:individualID, single_end:false, is_sra:false, type: 'reads', assemblies:tags ],
+        [ id:groupID, single_end:false, is_sra:false, type: readsType, assemblies:tags ],
         reads.first().collect { file(it, checkIfExists: true) }
     ]
 }
diff --git a/tests/merqury.fk/phased2x.mp/assemblysheet.csv b/tests/merqury.fk/phased2x.mp/assemblysheet.csv
new file mode 100644
index 00000000..5ae40b57
--- /dev/null
+++ b/tests/merqury.fk/phased2x.mp/assemblysheet.csv
@@ -0,0 +1,4 @@
+tag,fasta,reads_1,reads_2,maternal_reads_1,paternal_reads_1
+COL,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fasta,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_2.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_CVI.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_COL.fastq.gz
+CVI,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fasta,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_2.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_CVI.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_COL.fastq.gz
+FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,SRR8238189
diff --git a/tests/merqury.fk/phased2x.mp/assemblysheet.csv.bk b/tests/merqury.fk/phased2x.mp/assemblysheet.csv.bk
new file mode 100644
index 00000000..df1ed671
--- /dev/null
+++ b/tests/merqury.fk/phased2x.mp/assemblysheet.csv.bk
@@ -0,0 +1,3 @@
+tag,fasta,reads_1,maternal_reads_1,paternal_reads_1
+COL,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fasta,SRR3703081,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fastq.gz,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fastq.gz
+CVI,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fasta,SRR3703081,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fastq.gz,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fastq.gz
diff --git a/tests/merqury.fk/phased2x.mp/params.json b/tests/merqury.fk/phased2x.mp/params.json
new file mode 100644
index 00000000..bc483334
--- /dev/null
+++ b/tests/merqury.fk/phased2x.mp/params.json
@@ -0,0 +1,10 @@
+{
+    "config_profile_name": "Test profile",
+    "config_profile_description": "Merqury.FK test for a phased diploid assembly contained in separate fasta with parental reads",
+    "input": "tests/merqury.fk/phased2x.mp/assemblysheet.csv",
+    "merqury_skip": false,
+    "merqury_kmer_length": 21,
+    "max_cpus": 8,
+    "max_memory": "32.GB",
+    "max_time": "6.h"
+}
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index dc9b8990..e5a627b2 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -22,6 +22,9 @@ include { FQ2HIC                            } from '../subworkflows/local/fq2hic
 include { CAT_CAT as TAG_ASSEMBLY           } from '../modules/pfr/cat/cat/main'
 include { FASTA_SYNTENY                     } from '../subworkflows/local/fasta_synteny'
 include { FASTK_FASTK                       } from '../modules/nf-core/fastk/fastk/main'
+include { FASTK_FASTK as MATERNAL_FASTK     } from '../modules/nf-core/fastk/fastk/main'
+include { FASTK_FASTK as PATERNAL_FASTK     } from '../modules/nf-core/fastk/fastk/main'
+include { MERQURYFK_HAPMAKER                } from '../modules/local/merquryfk_hapmaker'
 include { MERQURYFK_MERQURYFK               } from '../modules/nf-core/merquryfk/merquryfk/main'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
@@ -40,6 +43,8 @@ workflow ASSEMBLYQC {
     ch_hic_reads
     ch_xref_assembly
     ch_reads
+    ch_maternal_reads
+    ch_paternal_reads
     ch_params_as_json
     ch_summary_params_as_json
 
@@ -271,28 +276,29 @@ workflow ASSEMBLYQC {
                                             | map { tag, fa -> [ [ id: tag ], fa ] }
 
     ch_hic_reads_branch                     = ch_hic_reads
-                                            | combine(ch_hic_input_assembly.first()) // Wait till first clean assembly arrives
+                                            | combine(ch_hic_input_assembly.first())
+                                            // Wait till first clean assembly arrives
                                             | map { meta, fq, meta2, fasta -> [ meta, fq ] }
                                             | branch { meta, fq ->
                                                 sra: meta.is_sra
                                                 rest: ! meta.is_sra
                                             }
     // Reads
+    ch_all_clean_assemblies                 = ch_clean_assembly_tagged
+                                            | map { [ it ] }
+                                            | collect
+                                            | map { [ it ] }
+
     ch_reads_assemblies                     = ch_reads
                                             | combine(
-                                                ch_clean_assembly_tagged
-                                                | map { [ it ] }
-                                                | collect
-                                                | map { [ it ] }
+                                                ch_all_clean_assemblies
                                             )
+                                            // This combine with the filter after map
+                                            // is a join on list of assembly tags
+                                            // such as [ tag1 ] or [ tag1, tag2 ]
                                             | map { meta, fq, assemblies ->
                                                 [
-                                                    [
-                                                        id: meta.id,
-                                                        single_end: meta.single_end,
-                                                        is_sra: meta.is_sra,
-                                                        type: meta.type
-                                                    ],
+                                                    meta,
                                                     fq,
                                                     assemblies
                                                         .findAll { meta2, fasta -> meta2.id in meta.assemblies }
@@ -301,6 +307,7 @@ workflow ASSEMBLYQC {
                                                         .sort(false)
                                                 ]
                                             }
+                                            | filter { meta, fq, fastas -> fastas }
 
     ch_reads_branch                         = ch_reads_assemblies
                                             | map { meta, fq, fastas -> [ meta, fq ] }
@@ -309,18 +316,76 @@ workflow ASSEMBLYQC {
                                                 rest: ! meta.is_sra
                                             }
 
+    // Maternal reads
+    ch_maternal_reads_branch                = ch_maternal_reads
+                                            | combine(
+                                                ch_all_clean_assemblies
+                                            )
+                                            // This combine/filter/map is used to sync the
+                                            // reads channel with clean_assembly channel
+                                            // so that the downstream modules wait for
+                                            // the upstream modules to complete first
+                                            | map { meta, fq, assemblies ->
+                                                [
+                                                    meta,
+                                                    fq,
+                                                    assemblies
+                                                        .findAll { meta2, fasta -> meta2.id in meta.assemblies }
+                                                        .collect { meta2, fasta -> fasta }
+                                                        .flatten()
+                                                        .sort(false)
+                                                ]
+                                            }
+                                            | filter { meta, fq, fastas -> fastas }
+                                            | map { meta, fq, assemblies -> [ meta, fq ] }
+                                            | branch { meta, fq ->
+                                                sra: meta.is_sra
+                                                rest: ! meta.is_sra
+                                            }
+
+    // Paternal reads
+    ch_paternal_reads_branch                = ch_paternal_reads
+                                            | combine(
+                                                ch_all_clean_assemblies
+                                            )
+                                            | map { meta, fq, assemblies ->
+                                                [
+                                                    meta,
+                                                    fq,
+                                                    assemblies
+                                                        .findAll { meta2, fasta -> meta2.id in meta.assemblies }
+                                                        .collect { meta2, fasta -> fasta }
+                                                        .flatten()
+                                                        .sort(false)
+                                                ]
+                                            }
+                                            | filter { meta, fq, fastas -> fastas }
+                                            | map { meta, fq, assemblies -> [ meta, fq ] }
+                                            | branch { meta, fq ->
+                                                sra: meta.is_sra
+                                                rest: ! meta.is_sra
+                                            }
+
     // MODULE: FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as FETCHNGS
     ch_fetchngs_inputs                      = ch_hic_reads_branch.sra
                                             | mix(ch_reads_branch.sra)
+                                            | mix(ch_maternal_reads_branch.sra)
     FETCHNGS(
-        ch_fetchngs_inputs,
+        ch_fetchngs_inputs.map { meta, sra -> [ [ id: meta.id, single_end: meta.single_end ], sra ] },
         []
     )
 
     ch_fetchngs                             = FETCHNGS.out.reads
+                                            | join(
+                                                ch_fetchngs_inputs
+                                                | map { meta, sra -> [ [ id: meta.id, single_end: meta.single_end ], meta ] }
+                                            )
+                                            | map { meta, fq, meta2 -> [ meta2, fq ] }
                                             | branch { meta, fq ->
-                                                hic: meta.type == 'hic'
-                                                reads: meta.type == 'reads'
+                                                hic:        meta.type == 'hic'
+                                                reads:      meta.type == 'reads'
+                                                maternal:   meta.type == 'maternal'
+                                                paternal:   meta.type == 'paternal'
                                             }
     ch_versions                             = ch_versions.mix(FETCHNGS.out.versions)
 
@@ -479,25 +544,131 @@ workflow ASSEMBLYQC {
     // MODULE: FASTK_FASTK
     ch_reads_files                          = ch_fetchngs.reads
                                             | mix(ch_reads_branch.rest)
+
     FASTK_FASTK ( ch_reads_files )
 
     ch_reads_fastk_hist                     = FASTK_FASTK.out.hist
     ch_reads_fastk                          = FASTK_FASTK.out.ktab
     ch_versions                             = ch_versions.mix(FASTK_FASTK.out.versions.first())
 
-    // MODULE: MERQURYFK_MERQURYFK
-    ch_merqury_assemblies                   = ch_reads_assemblies
-                                            | map { meta, fq, fastas -> [ meta, fastas ] }
+    // MODULE: FASTK_FASTK as MATERNAL_FASTK
+    ch_maternal_reads_files                 = ch_fetchngs.maternal
+                                            | mix(ch_maternal_reads_branch.rest)
+
+    MATERNAL_FASTK(
+        // Guard against failed resume on addition of assemblies with same parents
+        ch_maternal_reads_files
+        | map { meta, fq -> [ [ id: meta.id ], fq ] }
+    )
+
+    // ch_maternal_fastk_hist                  = MATERNAL_FASTK.out.hist
+    //                                         | join(
+    //                                             ch_maternal_reads_files
+    //                                             | map { meta, fq -> [ [ id: meta.id ], meta ] }
+    //                                         )
+    //                                         | map { meta, hist, meta2 -> [ meta2, hist ] }
+
+    ch_maternal_fastk                       = MATERNAL_FASTK.out.ktab
+                                            | join(
+                                                ch_maternal_reads_files
+                                                | map { meta, fq -> [ [ id: meta.id ], meta ] }
+                                            )
+                                            | map { meta, ktab, meta2 -> [ meta2, ktab ] }
+    ch_versions                             = ch_versions.mix(MATERNAL_FASTK.out.versions.first())
+
+    // MODULE: FASTK_FASTK as PATERNAL_FASTK
+    ch_paternal_reads_files                 = ch_fetchngs.paternal
+                                            | mix(ch_paternal_reads_branch.rest)
+
+    PATERNAL_FASTK(
+        ch_paternal_reads_files
+        | map { meta, fq -> [ [ id: meta.id ], fq ] }
+    )
+
+    // ch_paternal_fastk_hist                  = PATERNAL_FASTK.out.hist
+    //                                         | join(
+    //                                             ch_paternal_reads_files
+    //                                             | map { meta, fq -> [ [ id: meta.id ], meta ] }
+    //                                         )
+    //                                         | map { meta, hist, meta2 -> [ meta2, hist ] }
+
+    ch_paternal_fastk                       = PATERNAL_FASTK.out.ktab
+                                            | join(
+                                                ch_paternal_reads_files
+                                                | map { meta, fq -> [ [ id: meta.id ], meta ] }
+                                            )
+                                            | map { meta, ktab, meta2 -> [ meta2, ktab ] }
+    ch_versions                             = ch_versions.mix(PATERNAL_FASTK.out.versions.first())
+
+    // MODULE: MERQURYFK_HAPMAKER
+    ch_all_assemblies_with_parents          = ch_maternal_fastk
+                                            | mix(ch_paternal_fastk)
+                                            | flatMap { meta, ktab -> meta.assemblies }
+                                            | unique
+                                            | collect
+                                            | map { [ it ] }
+                                            | ifEmpty( [ [] ] )
 
+    ch_reads_fastk_without_parents          = ch_reads_fastk
+                                            | combine(
+                                                ch_all_assemblies_with_parents
+                                            )
+                                            | filter { meta, ktab, p_asms -> ! meta.assemblies.any { it in p_asms } }
+                                            | map { meta, ktab, p_asms -> [ meta, ktab, [], [] ] }
+
+    ch_group_fastk                          = ch_reads_fastk
+                                            | combine ( ch_maternal_fastk )
+                                            | filter { meta, ktab, meta2, mktab ->
+                                                meta.assemblies.every { it in meta2.assemblies }
+                                            }
+                                            | map { meta, ktab, meta2, mktab ->
+                                                [ meta, ktab, mktab ]
+                                            }
+                                            | combine ( ch_paternal_fastk )
+                                            | filter { meta, ktab, mktab, meta2, pktab ->
+                                                meta.assemblies.every { it in meta2.assemblies }
+                                            }
+                                            | map { meta, ktab, mktab, meta2, pktab ->
+                                                [ meta, ktab, mktab, pktab ]
+                                            }
+
+    MERQURYFK_HAPMAKER(
+        ch_group_fastk.map { meta, ktab, mktab, pktab -> [ meta, ktab ] },
+        ch_group_fastk.map { meta, ktab, mktab, pktab -> mktab },
+        ch_group_fastk.map { meta, ktab, mktab, pktab -> pktab }
+    )
+
+    ch_parental_hapmers                     = MERQURYFK_HAPMAKER.out.mat_hapmers
+                                            | join(MERQURYFK_HAPMAKER.out.pat_hapmers)
+    ch_versions                             = ch_versions.mix(MERQURYFK_HAPMAKER.out.versions.first())
+
+    // Prepare FASTK tables
+    ch_fastk_ktabs                          = ch_group_fastk
+                                            | join(ch_parental_hapmers)
+                                            | map { meta, ktab, mktab, pktab, hap_mktab, hap_pktab ->
+                                                [ meta, ktab, hap_mktab, hap_pktab ]
+                                            }
+                                            | mix(ch_reads_fastk_without_parents)
+                                            | map { meta, ktab, hap_mktab, hap_pktab ->
+                                                [
+                                                    meta,
+                                                    ktab + hap_mktab + hap_pktab
+                                                ]
+                                            }
+
+    // MODULE: MERQURYFK_MERQURYFK
     ch_merqury_inputs                       = ch_reads_fastk_hist
                                             | join(
-                                                ch_reads_fastk
+                                                ch_fastk_ktabs
                                             )
                                             | join(
-                                                ch_merqury_assemblies
+                                                ch_reads_assemblies
+                                                | map { meta, fq, fastas -> [ meta, fastas ] }
                                             )
                                             | map { meta, hist, ktab, fastas ->
-                                                [ meta, hist, ktab, fastas, [] ]
+                                                fastas.size() > 1
+                                                ? [ meta, hist, ktab, fastas[0], fastas[1] ]
+                                                : [ meta, hist, ktab, fastas[0], [] ]
                                             }
 
     MERQURYFK_MERQURYFK ( ch_merqury_inputs )
@@ -527,25 +698,25 @@ workflow ASSEMBLYQC {
                                             )
 
     // MODULE: CREATEREPORT
-    CREATEREPORT(
-        ch_invalid_assembly_log             .collect().ifEmpty([]),
-        ch_invalid_gff3_log                 .collect().ifEmpty([]),
-        ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
-        ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
-        ch_assemblathon_stats               .collect().ifEmpty([]),
-        ch_gt_stats                         .collect().ifEmpty([]),
-        ch_busco_outputs                    .collect().ifEmpty([]),
-        ch_busco_gff_outputs                .collect().ifEmpty([]),
-        ch_tidk_outputs                     .collect().ifEmpty([]),
-        ch_lai_outputs                      .collect().ifEmpty([]),
-        ch_kraken2_plot                     .collect().ifEmpty([]),
-        ch_hic_html                         .collect().ifEmpty([]),
-        ch_synteny_plots                    .collect().ifEmpty([]),
-        ch_merqury_outputs                  .collect().ifEmpty([]),
-        ch_versions_yml,
-        ch_params_as_json,
-        ch_summary_params_as_json
-    )
+    // CREATEREPORT(
+    //     ch_invalid_assembly_log             .collect().ifEmpty([]),
+    //     ch_invalid_gff3_log                 .collect().ifEmpty([]),
+    //     ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
+    //     ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
+    //     ch_assemblathon_stats               .collect().ifEmpty([]),
+    //     ch_gt_stats                         .collect().ifEmpty([]),
+    //     ch_busco_outputs                    .collect().ifEmpty([]),
+    //     ch_busco_gff_outputs                .collect().ifEmpty([]),
+    //     ch_tidk_outputs                     .collect().ifEmpty([]),
+    //     ch_lai_outputs                      .collect().ifEmpty([]),
+    //     ch_kraken2_plot                     .collect().ifEmpty([]),
+    //     ch_hic_html                         .collect().ifEmpty([]),
+    //     ch_synteny_plots                    .collect().ifEmpty([]),
+    //     ch_merqury_outputs                  .collect().ifEmpty([]),
+    //     ch_versions_yml,
+    //     ch_params_as_json,
+    //     ch_summary_params_as_json
+    // )
 }
 
 /*

From 60433a6d313d204b745a91f140272828fc139f48 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 22 May 2024 15:44:14 +1200
Subject: [PATCH 47/76] Migrated to merqury

---
 CHANGELOG.md                                  |    5 +-
 CITATIONS.md                                  |    4 +-
 README.md                                     |    8 +-
 bin/assemblyqc.py                             |    4 +-
 ...{merquryfk_parser.py => merqury_parser.py} |   35 +-
 bin/report_modules/templates/base.html        |    8 +-
 .../templates/merqury/dropdown.html           |   10 +
 .../templates/merqury/merqury.html            |   17 +
 .../templates/merqury/report_contents.html    |   61 +
 .../templates/merquryfk/dropdown.html         |   10 -
 .../templates/merquryfk/merquryfk.html        |   21 -
 .../templates/merquryfk/report_contents.html  |   36 -
 conf/modules.config                           |   21 +-
 docs/output.md                                |   10 +-
 docs/usage.md                                 |   18 +-
 modules.json                                  |   36 +-
 modules/local/createreport.nf                 |    2 +-
 modules/local/merquryfk_hapmaker.nf           |  101 --
 modules/nf-core/fastk/fastk/environment.yml   |    7 -
 modules/nf-core/fastk/fastk/fastk-fastk.diff  |  542 ---------
 modules/nf-core/fastk/fastk/main.nf           |   71 --
 modules/nf-core/fastk/fastk/meta.yml          |   50 -
 .../nf-core/fastk/fastk/tests/main.nf.test    |  110 --
 .../fastk/fastk/tests/main.nf.test.snap       |  346 ------
 .../nf-core/fastk/fastk/tests/nextflow.config |    3 -
 modules/nf-core/fastk/fastk/tests/tags.yml    |    2 -
 modules/nf-core/merqury/environment.yml       |    7 +
 modules/nf-core/merqury/main.nf               |   60 +
 modules/nf-core/merqury/meta.yml              |   98 ++
 .../merquryfk/merquryfk/environment.yml       |    7 -
 modules/nf-core/merquryfk/merquryfk/main.nf   |  101 --
 .../merquryfk/merquryfk-merquryfk.diff        | 1060 -----------------
 modules/nf-core/merquryfk/merquryfk/meta.yml  |  113 --
 .../merquryfk/merquryfk/tests/main.nf.test    |  153 ---
 .../merquryfk/tests/main.nf.test.snap         |  802 -------------
 .../merquryfk/merquryfk/tests/nextflow.config |   11 -
 .../merquryfk/merquryfk/tests/tags.yml        |    2 -
 modules/nf-core/meryl/count/environment.yml   |    7 +
 modules/nf-core/meryl/count/main.nf           |   53 +
 modules/nf-core/meryl/count/meta.yml          |   45 +
 .../nf-core/meryl/count/tests/main.nf.test    |   60 +
 .../meryl/count/tests/main.nf.test.snap       |  332 ++++++
 modules/nf-core/meryl/count/tests/tags.yml    |    2 +
 .../nf-core/meryl/unionsum/environment.yml    |    7 +
 modules/nf-core/meryl/unionsum/main.nf        |   49 +
 modules/nf-core/meryl/unionsum/meta.yml       |   43 +
 .../nf-core/meryl/unionsum/tests/main.nf.test |  123 ++
 .../meryl/unionsum/tests/main.nf.test.snap    |  627 ++++++++++
 modules/nf-core/meryl/unionsum/tests/tags.yml |    2 +
 .../fasterqdump/sratools-fasterqdump.diff     |  252 ----
 .../sratools/prefetch/sratools-prefetch.diff  |  112 --
 modules/pfr/merqury/hapmers/environment.yml   |    9 +
 modules/pfr/merqury/hapmers/main.nf           |   96 ++
 modules/pfr/merqury/hapmers/meta.yml          |   70 ++
 .../pfr/merqury/hapmers/tests/main.nf.test    |  113 ++
 .../merqury/hapmers/tests/main.nf.test.snap   |   64 +
 modules/pfr/merqury/hapmers/tests/tags.yml    |    2 +
 .../utils_nfcore_assemblyqc_pipeline/main.nf  |    3 +-
 tests/merqury.fk/phased2x.mp/params.json      |   10 -
 .../mixed2x/assemblysheet.csv                 |    0
 .../mixed2x/params.json                       |    4 +-
 .../phased2x.mp/assemblysheet.csv}            |    0
 .../phased2x.mp/assemblysheet.csv.local}      |    2 +-
 tests/merqury/phased2x.mp/params.json         |   10 +
 .../phased2x/assemblysheet.csv                |    0
 .../phased2x/params.json                      |    4 +-
 workflows/assemblyqc.nf                       |  237 ++--
 67 files changed, 2187 insertions(+), 4103 deletions(-)
 rename bin/report_modules/parsers/{merquryfk_parser.py => merqury_parser.py} (64%)
 create mode 100644 bin/report_modules/templates/merqury/dropdown.html
 create mode 100644 bin/report_modules/templates/merqury/merqury.html
 create mode 100644 bin/report_modules/templates/merqury/report_contents.html
 delete mode 100644 bin/report_modules/templates/merquryfk/dropdown.html
 delete mode 100644 bin/report_modules/templates/merquryfk/merquryfk.html
 delete mode 100644 bin/report_modules/templates/merquryfk/report_contents.html
 delete mode 100644 modules/local/merquryfk_hapmaker.nf
 delete mode 100644 modules/nf-core/fastk/fastk/environment.yml
 delete mode 100644 modules/nf-core/fastk/fastk/fastk-fastk.diff
 delete mode 100644 modules/nf-core/fastk/fastk/main.nf
 delete mode 100644 modules/nf-core/fastk/fastk/meta.yml
 delete mode 100644 modules/nf-core/fastk/fastk/tests/main.nf.test
 delete mode 100644 modules/nf-core/fastk/fastk/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/fastk/fastk/tests/nextflow.config
 delete mode 100644 modules/nf-core/fastk/fastk/tests/tags.yml
 create mode 100644 modules/nf-core/merqury/environment.yml
 create mode 100644 modules/nf-core/merqury/main.nf
 create mode 100644 modules/nf-core/merqury/meta.yml
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/environment.yml
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/main.nf
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/meta.yml
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
 delete mode 100644 modules/nf-core/merquryfk/merquryfk/tests/tags.yml
 create mode 100644 modules/nf-core/meryl/count/environment.yml
 create mode 100644 modules/nf-core/meryl/count/main.nf
 create mode 100644 modules/nf-core/meryl/count/meta.yml
 create mode 100644 modules/nf-core/meryl/count/tests/main.nf.test
 create mode 100644 modules/nf-core/meryl/count/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/meryl/count/tests/tags.yml
 create mode 100644 modules/nf-core/meryl/unionsum/environment.yml
 create mode 100644 modules/nf-core/meryl/unionsum/main.nf
 create mode 100644 modules/nf-core/meryl/unionsum/meta.yml
 create mode 100644 modules/nf-core/meryl/unionsum/tests/main.nf.test
 create mode 100644 modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/meryl/unionsum/tests/tags.yml
 delete mode 100644 modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff
 delete mode 100644 modules/nf-core/sratools/prefetch/sratools-prefetch.diff
 create mode 100644 modules/pfr/merqury/hapmers/environment.yml
 create mode 100644 modules/pfr/merqury/hapmers/main.nf
 create mode 100644 modules/pfr/merqury/hapmers/meta.yml
 create mode 100644 modules/pfr/merqury/hapmers/tests/main.nf.test
 create mode 100644 modules/pfr/merqury/hapmers/tests/main.nf.test.snap
 create mode 100644 modules/pfr/merqury/hapmers/tests/tags.yml
 delete mode 100644 tests/merqury.fk/phased2x.mp/params.json
 rename tests/{merqury.fk => merqury}/mixed2x/assemblysheet.csv (100%)
 rename tests/{merqury.fk => merqury}/mixed2x/params.json (50%)
 rename tests/{merqury.fk/phased2x.mp/assemblysheet.csv.bk => merqury/phased2x.mp/assemblysheet.csv} (100%)
 rename tests/{merqury.fk/phased2x.mp/assemblysheet.csv => merqury/phased2x.mp/assemblysheet.csv.local} (84%)
 create mode 100644 tests/merqury/phased2x.mp/params.json
 rename tests/{merqury.fk => merqury}/phased2x/assemblysheet.csv (100%)
 rename tests/{merqury.fk => merqury}/phased2x/params.json (50%)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f25f4641..d9feb3ed 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0+dev - [17-May-2024]
+## v2.0.0+dev - [22-May-2024]
 
 ### `Added`
 
@@ -18,11 +18,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 9. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
 10. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
 11. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
-12. Added `MERQURYFK` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
+12. Added `MERQURY` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
 13. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
 14. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
 15. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
-16. Locally added stubs for `FASTK/FASTK`, `MERQURYFK/MERQURYFK`, `SRATOOLS/FASTERQDUMP` and `SRATOOLS/PREFETCH`
 
 ### `Fixed`
 
diff --git a/CITATIONS.md b/CITATIONS.md
index 4de821db..6ee2a4f5 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -116,10 +116,8 @@
 
   > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: <https://doi.org/10.1371/journal.pcbi.1005944>. PMID: 29373581; PMCID: PMC5802927.
 
-- MERQURYFK, [Custom Permissive License](https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE)
+- MERQURY, [United States Government Work](https://github.com/marbl/merqury?tab=License-1-ov-file#readme)
 
-  > https://github.com/thegenemyers/MERQURY.FK
-  >
   > Rhie, A., Walenz, B.P., Koren, S. et al. 2020. Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies. Genome Biol 21, 245. doi: <https://doi.org/10.1186/s13059-020-02134-9>
 
 ## Software packaging/containerisation tools
diff --git a/README.md b/README.md
index f30501ce..30ec1d32 100644
--- a/README.md
+++ b/README.md
@@ -10,7 +10,7 @@
 
 ## Introduction
 
-**plant-food-research-open/assemblyqc** is a [NextFlow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their version are listed in [CITATIONS.md](./CITATIONS.md).
+**plant-food-research-open/assemblyqc** is a [NextFlow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their references are listed in [CITATIONS.md](./CITATIONS.md).
 
 ## Pipeline Flowchart
 
@@ -37,7 +37,7 @@ flowchart LR
   Run --> KRAKEN2
   Run --> HIC_CONTACT_MAP[HIC CONTACT MAP]
   Run --> SYNTENY
-  Run --> MERQURYFK
+  Run --> MERQURY
 
   ASS_STATS --> REPORT
   GFF_STATS --> REPORT
@@ -47,7 +47,7 @@ flowchart LR
   KRAKEN2 --> REPORT
   HIC_CONTACT_MAP --> REPORT
   SYNTENY --> REPORT
-  MERQURYFK --> REPORT
+  MERQURY --> REPORT
 ```
 
 - [FASTA VALIDATION](https://github.com/GallVp/fasta_validator)
@@ -62,7 +62,7 @@ flowchart LR
 - [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification
 - [HIC CONTACT MAP](https://github.com/igvteam/juicebox.js): Alignment and visualisation of HiC data
 - SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer), [CIRCOS](http://circos.ca/documentation/) and [PLOTLY](https://plotly.com)
-- [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK): K-mer completeness, consensus quality and phasing assessment
+- [MERQURY](https://github.com/marbl/merqury): K-mer completeness, consensus quality and phasing assessment
 
 ## Usage
 
diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py
index f5d9d6bc..b5c73f56 100755
--- a/bin/assemblyqc.py
+++ b/bin/assemblyqc.py
@@ -25,7 +25,7 @@
 from report_modules.parsers.kraken2_parser import parse_kraken2_folder
 from report_modules.parsers.hic_parser import parse_hic_folder
 from report_modules.parsers.synteny_parser import parse_synteny_folder
-from report_modules.parsers.merquryfk_parser import parse_merquryfk_folder
+from report_modules.parsers.merqury_parser import parse_merqury_folder
 
 if __name__ == "__main__":
     params_dict, params_table = parse_params_json("params_json.json")
@@ -52,7 +52,7 @@
     data_from_tools = {**data_from_tools, **parse_kraken2_folder()}
     data_from_tools = {**data_from_tools, **parse_hic_folder()}
     data_from_tools = {**data_from_tools, **parse_synteny_folder()}
-    data_from_tools = {**data_from_tools, **parse_merquryfk_folder()}
+    data_from_tools = {**data_from_tools, **parse_merqury_folder()}
 
     with open("software_versions.yml", "r") as f:
         versions_from_ch_versions = yaml.safe_load(f)
diff --git a/bin/report_modules/parsers/merquryfk_parser.py b/bin/report_modules/parsers/merqury_parser.py
similarity index 64%
rename from bin/report_modules/parsers/merquryfk_parser.py
rename to bin/report_modules/parsers/merqury_parser.py
index 22f8be27..6ba0b8e7 100644
--- a/bin/report_modules/parsers/merquryfk_parser.py
+++ b/bin/report_modules/parsers/merqury_parser.py
@@ -1,6 +1,5 @@
 import base64
 import os
-import re
 
 from report_modules.parsers.parsing_commons import sort_list_of_results
 from tabulate import tabulate
@@ -8,7 +7,11 @@
 import pandas as pd
 
 
-def load_image_as_base64_str(file_name):
+def load_image_as_base64_str(file_name, optional=False):
+
+    if optional and not os.path.exists(file_name):
+        return None
+
     with open(file_name, "rb") as f:
         binary_fc = f.read()
 
@@ -16,14 +19,14 @@ def load_image_as_base64_str(file_name):
     return f"data:image/png+xml;base64,{base64_utf8_str}"
 
 
-def parse_merquryfk_folder(folder_name="merquryfk_outputs"):
+def parse_merqury_folder(folder_name="merqury_outputs"):
     dir = os.getcwdb().decode()
     merqury_folder_path = Path(f"{dir}/{folder_name}")
 
     if not os.path.exists(merqury_folder_path):
         return {}
 
-    data = {"MERQURYFK": []}
+    data = {"MERQURY": []}
 
     completeness_stats_paths = [
         item for item in merqury_folder_path.glob("*.completeness.stats")
@@ -31,16 +34,15 @@ def parse_merquryfk_folder(folder_name="merquryfk_outputs"):
 
     for completeness_stats_path in completeness_stats_paths:
 
-        data_id = os.path.basename(str(completeness_stats_path)).split(
+        individual_id = os.path.basename(str(completeness_stats_path)).split(
             ".completeness.stats"
         )[0]
-        individual_id = data_id.split("-reads-")[0]
         haplotypes = individual_id.split("-and-")
 
-        completeness_stats_table = pd.read_csv(completeness_stats_path, sep="\t")
-        qv_stats_table = pd.read_csv(f"{folder_name}/{data_id}.qv", sep="\t")
+        completeness_stats_table = pd.read_csv(completeness_stats_path, sep="\t", header=None)
+        qv_stats_table = pd.read_csv(f"{folder_name}/{individual_id}.qv", sep="\t", header=None)
 
-        data["MERQURYFK"].append(
+        data["MERQURY"].append(
             {
                 "individual_id": individual_id,
                 "completeness_stats_table": completeness_stats_table.to_dict("records"),
@@ -59,16 +61,25 @@ def parse_merquryfk_folder(folder_name="merquryfk_outputs"):
                     numalign="left",
                     showindex=False,
                 ),
-                "plots": [
+                "hap_plots": [
                     {
                         "hap": hap,
                         "plot": load_image_as_base64_str(
-                            f"{folder_name}/{data_id}.{hap}.spectra-cn.fl.png"
+                            f"{folder_name}/{individual_id}.{hap}.spectra-cn.fl.png"
                         ),
                     }
                     for hap in haplotypes
                 ],
+                "asm_plot": load_image_as_base64_str(
+                    f"{folder_name}/{individual_id}.spectra-asm.fl.png"
+                ),
+                "plot": load_image_as_base64_str(
+                    f"{folder_name}/{individual_id}.spectra-cn.fl.png", True
+                ),
+                "hapmers_blob": load_image_as_base64_str(
+                    f"{folder_name}/{individual_id}.hapmers.blob.png", True
+                ),
             }
         )
 
-    return {"MERQURYFK": sort_list_of_results(data["MERQURYFK"], "individual_id")}
+    return {"MERQURY": sort_list_of_results(data["MERQURY"], "individual_id")}
diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html
index d2478dfb..b570d8e5 100644
--- a/bin/report_modules/templates/base.html
+++ b/bin/report_modules/templates/base.html
@@ -68,8 +68,8 @@
     <button class="tablinks" onclick="openTool(event, 'SYNTENY_LINEAR')">SYNTENY LINEAR</button>
     {% endif %}
 
-    {% if 'MERQURYFK' in all_stats_dicts %}
-    <button class="tablinks" onclick="openTool(event, 'MERQURYFK')">MERQURYFK</button>
+    {% if 'MERQURY' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'MERQURY')">MERQURY</button>
     {% endif %}
   </div>
 
@@ -132,8 +132,8 @@
   {% include 'synteny_linear/synteny_linear.html' %}
   {% endif %}
 
-  {% if 'MERQURYFK' in all_stats_dicts %}
-  {% include 'merquryfk/merquryfk.html' %}
+  {% if 'MERQURY' in all_stats_dicts %}
+  {% include 'merqury/merqury.html' %}
   {% endif %}
 </body>
 {% include 'js.html' %}
diff --git a/bin/report_modules/templates/merqury/dropdown.html b/bin/report_modules/templates/merqury/dropdown.html
new file mode 100644
index 00000000..5c61274c
--- /dev/null
+++ b/bin/report_modules/templates/merqury/dropdown.html
@@ -0,0 +1,10 @@
+<div class="dropdown">
+  <div class="dropdown_content">
+    <select id="selector_MERQURY" onchange="showContent('MERQURY')">
+      {% set str_individual_id = 'individual_id' %} {% for item in range(all_stats_dicts["MERQURY"]|length) %}
+      <option value="tabcontent_MERQURY_{{all_stats_dicts['MERQURY'][item]['individual_id']}}">
+        {{ all_stats_dicts['MERQURY'][item][str_individual_id] }} {% endfor %}
+      </option>
+    </select>
+  </div>
+</div>
diff --git a/bin/report_modules/templates/merqury/merqury.html b/bin/report_modules/templates/merqury/merqury.html
new file mode 100644
index 00000000..0090e222
--- /dev/null
+++ b/bin/report_modules/templates/merqury/merqury.html
@@ -0,0 +1,17 @@
+<div id="MERQURY" class="tabcontent" style="display: none">
+  <div class="section-para-wrapper">
+    <p class="section-para">Often, genome assembly projects have illumina whole genome sequencing reads available for
+      the assembled individual. The k-mer spectrum of this read set can be used for independently evaluating assembly
+      quality without the need of a high quality reference. Merqury provides a set of tools for this purpose.</p>
+    <p class="section-para"><b>References:</b></p>
+    <p class="section-para">
+      Rhie, A., Walenz, B.P., Koren, S. et al. Merqury: reference-free quality, completeness, and phasing assessment for
+      genome assemblies. Genome Biol 21, 245 (2020). doi: <a href="https://doi.org/10.1186/s13059-020-02134-9"
+        target="_blank">10.1186/s13059-020-02134-9</a>
+    </p>
+    <p class="section-para">
+      <b>Version: {{ all_stats_dicts['VERSIONS']['MERQURY']['merqury'] }}</b>
+    </p>
+  </div>
+  {% include 'merqury/dropdown.html' %} {% include 'merqury/report_contents.html' %}
+</div>
diff --git a/bin/report_modules/templates/merqury/report_contents.html b/bin/report_modules/templates/merqury/report_contents.html
new file mode 100644
index 00000000..63ec32d8
--- /dev/null
+++ b/bin/report_modules/templates/merqury/report_contents.html
@@ -0,0 +1,61 @@
+{% set vars = {'is_first': True} %}
+{% for item in range(all_stats_dicts["MERQURY"]|length) %}
+{% set active_text = 'display: block' if vars.is_first else 'display: none' %}
+<div id="tabcontent_MERQURY_{{ all_stats_dicts['MERQURY'][item]['individual_id'] }}" class="tabcontent-MERQURY"
+  style="{{ active_text }}">
+  <div class="results-section">
+    <div class="section-heading-wrapper">
+      <div class="section-heading">{{ all_stats_dicts['MERQURY'][item]['individual_id'] }}</div>
+    </div>
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Completeness stats</b></p>
+    </div>
+    <div class="table-outer">
+      <div class="table-wrapper">{{ all_stats_dicts['MERQURY'][item]['completeness_stats_table_html'] }}</div>
+    </div>
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Consensus quality QV stats</b></p>
+    </div>
+    <div class="table-outer">
+      <div class="table-wrapper">{{ all_stats_dicts['MERQURY'][item]['qv_stats_table_html'] }}</div>
+    </div>
+
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Spectra-asm</b></p>
+    </div>
+    <div class="image-wrapper">
+      <img src="{{ all_stats_dicts['MERQURY'][item]['asm_plot'] }}" alt="" />
+    </div>
+
+    {% for subitem in range(all_stats_dicts["MERQURY"][item]['hap_plots']|length) %}
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>{{ all_stats_dicts['MERQURY'][item]['hap_plots'][subitem]['hap'] }} spectra-cn</b></p>
+    </div>
+    <div class="image-wrapper">
+      <img src="{{ all_stats_dicts['MERQURY'][item]['hap_plots'][subitem]['plot'] }}" alt="" />
+    </div>
+    {% endfor %}
+
+    {% if all_stats_dicts['MERQURY'][item]['plot'] is not none %}
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Spectra-cn</b></p>
+    </div>
+    <div class="image-wrapper">
+      <img src="{{ all_stats_dicts['MERQURY'][item]['plot'] }}" alt="" />
+    </div>
+    {% endif %}
+
+    {% if all_stats_dicts['MERQURY'][item]['hapmers_blob'] is not none %}
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Hapmers Blob</b></p>
+    </div>
+    <div class="image-wrapper">
+      <img src="{{ all_stats_dicts['MERQURY'][item]['hapmers_blob'] }}" alt="" />
+    </div>
+    {% endif %}
+
+  </div>
+</div>
+{% if vars.update({'is_first': False}) %}
+{% endif %}
+{% endfor %}
diff --git a/bin/report_modules/templates/merquryfk/dropdown.html b/bin/report_modules/templates/merquryfk/dropdown.html
deleted file mode 100644
index d257b4e9..00000000
--- a/bin/report_modules/templates/merquryfk/dropdown.html
+++ /dev/null
@@ -1,10 +0,0 @@
-<div class="dropdown">
-  <div class="dropdown_content">
-    <select id="selector_MERQURYFK" onchange="showContent('MERQURYFK')">
-      {% set str_individual_id = 'individual_id' %} {% for item in range(all_stats_dicts["MERQURYFK"]|length) %}
-      <option value="tabcontent_MERQURYFK_{{all_stats_dicts['MERQURYFK'][item]['individual_id']}}">
-        {{ all_stats_dicts['MERQURYFK'][item][str_individual_id] }} {% endfor %}
-      </option>
-    </select>
-  </div>
-</div>
diff --git a/bin/report_modules/templates/merquryfk/merquryfk.html b/bin/report_modules/templates/merquryfk/merquryfk.html
deleted file mode 100644
index 8331fbd3..00000000
--- a/bin/report_modules/templates/merquryfk/merquryfk.html
+++ /dev/null
@@ -1,21 +0,0 @@
-<div id="MERQURYFK" class="tabcontent" style="display: none">
-  <div class="section-para-wrapper">
-    <p class="section-para">The original Merqury is a collection of R, Java, and shell scripts for producing k-mer
-      analysis plots of genomic sequence data and assemblies with meryl as its core k-mer counter infra-structure.
-      MerquryFK replaces meryl with the FastK k-mer counter suite to considerably speed up analyses.</p>
-    <p class="section-para"><b>References:</b></p>
-    <p class="section-para">
-      <a href="https://github.com/thegenemyers/MERQURY.FK"
-        target="_blank">https://github.com/thegenemyers/MERQURY.FK</a>
-    </p>
-    <p class="section-para">
-      Rhie, A., Walenz, B.P., Koren, S. et al. Merqury: reference-free quality, completeness, and phasing assessment for
-      genome assemblies. Genome Biol 21, 245 (2020). doi: <a href="https://doi.org/10.1186/s13059-020-02134-9"
-        target="_blank">10.1186/s13059-020-02134-9</a>
-    </p>
-    <p class="section-para">
-      <b>Version: {{ all_stats_dicts['VERSIONS']['MERQURYFK_MERQURYFK']['merquryfk'] }}</b>
-    </p>
-  </div>
-  {% include 'merquryfk/dropdown.html' %} {% include 'merquryfk/report_contents.html' %}
-</div>
diff --git a/bin/report_modules/templates/merquryfk/report_contents.html b/bin/report_modules/templates/merquryfk/report_contents.html
deleted file mode 100644
index 6578a6f4..00000000
--- a/bin/report_modules/templates/merquryfk/report_contents.html
+++ /dev/null
@@ -1,36 +0,0 @@
-{% set vars = {'is_first': True} %}
-{% for item in range(all_stats_dicts["MERQURYFK"]|length) %}
-{% set active_text = 'display: block' if vars.is_first else 'display: none' %}
-<div id="tabcontent_MERQURYFK_{{ all_stats_dicts['MERQURYFK'][item]['individual_id'] }}" class="tabcontent-MERQURYFK"
-  style="{{ active_text }}">
-  <div class="results-section">
-    <div class="section-heading-wrapper">
-      <div class="section-heading">{{ all_stats_dicts['MERQURYFK'][item]['individual_id'] }}</div>
-    </div>
-    <div class="section-para-wrapper">
-      <p class="section-para"><b>Completeness stats</b></p>
-    </div>
-    <div class="table-outer">
-      <div class="table-wrapper">{{ all_stats_dicts['MERQURYFK'][item]['completeness_stats_table_html'] }}</div>
-    </div>
-    <div class="section-para-wrapper">
-      <p class="section-para"><b>Consensus quality QV stats</b></p>
-    </div>
-    <div class="table-outer">
-      <div class="table-wrapper">{{ all_stats_dicts['MERQURYFK'][item]['qv_stats_table_html'] }}</div>
-    </div>
-
-    {% for subitem in range(all_stats_dicts["MERQURYFK"][item]['plots']|length) %}
-    <div class="section-para-wrapper">
-      <p class="section-para"><b>{{ all_stats_dicts['MERQURYFK'][item]['plots'][subitem]['hap'] }} spectra-cn</b></p>
-    </div>
-    <div class="image-wrapper">
-      <img src="{{ all_stats_dicts['MERQURYFK'][item]['plots'][subitem]['plot'] }}" alt="" />
-    </div>
-    {% endfor %}
-
-  </div>
-</div>
-{% if vars.update({'is_first': False}) %}
-{% endif %}
-{% endfor %}
diff --git a/conf/modules.config b/conf/modules.config
index 04f77eca..783e6650 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -326,27 +326,12 @@ process {
         ext.prefix = { "${meta.id}.fasta" }
     }
 
-    withName: FASTK_FASTK {
-        ext.args = "-k$params.merqury_kmer_length -t1"
-    }
-
-    withName: MERQURYFK_MERQURYFK {
-        ext.args = '-v'
+    withName: MERQURY {
         publishDir = [
             [
-                path: { "${params.outdir}/merquryfk/${meta.id}" },
-                mode: params.publish_dir_mode,
-                pattern: '*.qv'
-            ],
-            [
-                path: { "${params.outdir}/merquryfk/${meta.id}" },
-                mode: params.publish_dir_mode,
-                pattern: '*.completeness.stats'
-            ],
-            [
-                path: { "${params.outdir}/merquryfk/${meta.id}" },
+                path: { "${params.outdir}/merqury/${meta.id}" },
                 mode: params.publish_dir_mode,
-                pattern: '*.*.spectra-cn.fl.png'
+                saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
             ]
         ]
     }
diff --git a/docs/output.md b/docs/output.md
index 030ac264..15ee0559 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -23,7 +23,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [Kraken2](#kraken2)
 - [HiC contact map](#hic-contact-map)
 - [Synteny](#synteny)
-- [Merqury.FK](#merquryfk)
+- [Merqury](#merqury)
 - [Pipeline information](#pipeline-information)
 
 ### FASTA and GFF3 validation
@@ -204,19 +204,19 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 <div align="center"><img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
 <div align="center"><img src="images/synteny_linear.png" alt="AssemblyQC - Linear synteny plot" width="50%"><hr><em>AssemblyQC - Linear synteny plot</em></div>
 
-### Merqury.FK
+### Merqury
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `merquryfk/`
-  - `tag1-and-tag2-reads-*`: Results folder for haplotype `tag1` and `tag2`.
+- `merqury/`
+  - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`.
     - `*.completeness.stats`: Assembly completeness statistics
     - `*.qv`: Assembly consensus quality QV statistics
     - `*.*.spectra-cn.fl.png`: Spectra-cn plot
     </details>
 
-[MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) is used for the k-mer analysis.
+[MERQURY](https://github.com/marbl/merqury) is used for the k-mer analysis.
 
 <div align="center"><img src="images/spectra_cn.png" alt="AssemblyQC - Spectra-cn plot" width="50%"><hr><em>AssemblyQC - Spectra-cn plot</em></div>
 
diff --git a/docs/usage.md b/docs/usage.md
index f262522f..1d803b63 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -10,7 +10,7 @@ You will need to create an assemblysheet with information about the assemblies y
 - `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
 - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) when performing synteny analysis
 
-See the [Merqury.FK](#merquryfk-k-mer-analysis) section For description of assemblysheet columns related to k-mer analysis with Merqury.FK.
+See the [Merqury](#merqury-k-mer-analysis) section For description of assemblysheet columns related to k-mer analysis with Merqury.
 
 ## External databases
 
@@ -85,19 +85,23 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
   - `fasta:` FASTA file
   - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column)
 
-### Merqury.FK K-mer analysis
+### Merqury K-mer analysis
 
 #### Additional assemblysheet columns
 
-- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY.FK](https://github.com/thegenemyers/MERQURY.FK) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY.FK. A genome can only have one or two haplotypes.
+- `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY](https://github.com/marbl/merqury) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY. A genome can only have one or two haplotypes.
 - `reads_2 [Optional]`: This column lists the second file if paired reads are used. If `reads_1` is a SRA ID, this column is ignored.
+- `maternal_reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to maternal reads. If two assemblies are haplotypes of the same genome, this path should be repeated. Moreover, more than one genome can have same `maternal_reads_1`.
+- `maternal_reads_2 [Optional]`: This column lists the second file if paired reads are used. If `maternal_reads_1` is a SRA ID, this column is ignored.
+- `paternal_reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to paternal reads. If two assemblies are haplotypes of the same genome, this path should be repeated. Moreover, more than one genome can have same `paternal_reads_1`.
+- `paternal_reads_2 [Optional]`: This column lists the second file if paired reads are used. If `paternal_reads_1` is a SRA ID, this column is ignored.
 
-See following assemblysheet examples for MERQURY.FK analysis.
+See following assemblysheet examples for MERQURY analysis.
 
 - [assemblysheet - 1x](../assets/assemblysheetv2.csv)
-- [assemblysheet - mixed2x](../tests/merqury.fk/mixed2x/assemblysheet.csv)
-- [assemblysheet - phased2x](../tests/merqury.fk/phased2x/assemblysheet.csv)
-- [assemblysheet - phased2x with parent reads](../tests/merqury.fk/phased2x.mp/assemblysheet.csv)
+- [assemblysheet - mixed2x](../tests/merqury/mixed2x/assemblysheet.csv)
+- [assemblysheet - phased2x](../tests/merqury/phased2x/assemblysheet.csv)
+- [assemblysheet - phased2x with parent reads](../tests/merqury/phased2x.mp/assemblysheet.csv)
 
 #### Parameters
 
diff --git a/modules.json b/modules.json
index f16ccf45..188bf227 100644
--- a/modules.json
+++ b/modules.json
@@ -80,6 +80,11 @@
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
+                    "merqury/hapmers": {
+                        "branch": "main",
+                        "git_sha": "46b70684b6ae0ac60cae154c61796b293707db64",
+                        "installed_by": ["modules"]
+                    },
                     "samblaster": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
@@ -130,12 +135,6 @@
                         "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
                         "installed_by": ["modules"]
                     },
-                    "fastk/fastk": {
-                        "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/fastk/fastk/fastk-fastk.diff"
-                    },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
@@ -156,11 +155,20 @@
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
                         "installed_by": ["modules"]
                     },
-                    "merquryfk/merquryfk": {
+                    "merqury": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff"
+                        "installed_by": ["modules"]
+                    },
+                    "meryl/count": {
+                        "branch": "master",
+                        "git_sha": "c95215baf20e6d8cf2ae0e8411d03c5d9ef21d4a",
+                        "installed_by": ["modules"]
+                    },
+                    "meryl/unionsum": {
+                        "branch": "master",
+                        "git_sha": "b7f584e61c35c138a5717f837f4da9b838e290a7",
+                        "installed_by": ["modules"]
                     },
                     "seqkit/seq": {
                         "branch": "master",
@@ -174,15 +182,13 @@
                     },
                     "sratools/fasterqdump": {
                         "branch": "master",
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"],
-                        "patch": "modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff"
+                        "git_sha": "dd935314930f972117455672f89e5cb0794719e5",
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"]
                     },
                     "sratools/prefetch": {
                         "branch": "master",
-                        "git_sha": "1fc29f92e439d5631fdf34b8ac4687297d70f5ec",
-                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"],
-                        "patch": "modules/nf-core/sratools/prefetch/sratools-prefetch.diff"
+                        "git_sha": "dd935314930f972117455672f89e5cb0794719e5",
+                        "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"]
                     },
                     "tidk/explore": {
                         "branch": "master",
diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf
index b072b184..fd53edc0 100644
--- a/modules/local/createreport.nf
+++ b/modules/local/createreport.nf
@@ -18,7 +18,7 @@ process CREATEREPORT {
     path kraken2_outputs            , stageAs: 'kraken2_outputs/*'
     path hic_outputs                , stageAs: 'hic_outputs/*'
     path synteny_outputs            , stageAs: 'synteny_outputs/*'
-    path merqury_outputs            , stageAs: 'merquryfk_outputs/*'
+    path merqury_outputs            , stageAs: 'merqury_outputs/*'
     path versions
     val params_json
     val params_summary_json
diff --git a/modules/local/merquryfk_hapmaker.nf b/modules/local/merquryfk_hapmaker.nf
deleted file mode 100644
index 311a3b79..00000000
--- a/modules/local/merquryfk_hapmaker.nf
+++ /dev/null
@@ -1,101 +0,0 @@
-process MERQURYFK_HAPMAKER {
-    tag "$meta.id"
-    label 'process_medium'
-
-    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.2'
-
-    input:
-    tuple val(meta), path(ktab)
-    path(mktab)
-    path(pktab)
-
-    output:
-    tuple val(meta), path('*_mat.hap.ktab*', hidden: true)  , emit: mat_hapmers
-    tuple val(meta), path('*_pat.hap.ktab*', hidden: true)  , emit: pat_hapmers
-    path "versions.yml"                                     , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "MERQURYFK_HAPMAKER module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args            = task.ext.args ?: ''
-    def prefix          = task.ext.prefix ?: "${meta.id}"
-    def mktab_file      = mktab.find { it.toString().endsWith(".ktab") }
-    def pktab_file      = pktab.find { it.toString().endsWith(".ktab") }
-    def ktab_file       = ktab.find  { it.toString().endsWith(".ktab") }
-    def mktab_basename  = mktab_file.baseName
-    def pktab_basename  = pktab_file.baseName
-    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    HAPmaker \\
-        $args \\
-        -T$task.cpus \\
-        $mktab_file \\
-        $pktab_file \\
-        $ktab_file
-
-    mv ${mktab_basename}.hap.ktab ${prefix}_mat.hap.ktab
-
-    find . -name ".${mktab_basename}.hap.*" \\
-    | while read -r file; do
-        new_name=\$(echo "\$file" | sed "s/${mktab_basename}/${prefix}_mat/")
-        mv "\$file" "\$new_name"
-    done
-
-    mv ${pktab_basename}.hap.ktab ${prefix}_pat.hap.ktab
-
-    find . -name ".${pktab_basename}.hap.*" \\
-    | while read -r file; do
-        new_name=\$(echo "\$file" | sed "s/${pktab_basename}/${prefix}_pat/")
-        mv "\$file" "\$new_name"
-    done
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastk: $FASTK_VERSION
-        merquryfk: $MERQURY_VERSION
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "MERQURYFK_HAPMAKER module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args            = task.ext.args ?: ''
-    def prefix          = task.ext.prefix ?: "${meta.id}"
-    def mktab_file      = mktab.find { it.toString().endsWith(".ktab") }
-    def pktab_file      = pktab.find { it.toString().endsWith(".ktab") }
-    def ktab_file       = ktab.find  { it.toString().endsWith(".ktab") }
-    def mktab_basename  = mktab_file.baseName
-    def pktab_basename  = pktab_file.baseName
-    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    echo \\
-    "HAPmaker \\
-        $args \\
-        -T$task.cpus \\
-        $mktab_file \\
-        $pktab_file \\
-        $ktab_file"
-
-    touch ${prefix}_mat.hap.ktab
-    touch .${prefix}_mat.hap.ktab.1
-
-    touch ${prefix}_pat.hap.ktab
-    touch .${prefix}_pat.hap.ktab.1
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastk: $FASTK_VERSION
-        merquryfk: $MERQURY_VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/fastk/fastk/environment.yml b/modules/nf-core/fastk/fastk/environment.yml
deleted file mode 100644
index efb503e1..00000000
--- a/modules/nf-core/fastk/fastk/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: fastk_fastk
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::false_flag # False flag to pass nf-core/lint
diff --git a/modules/nf-core/fastk/fastk/fastk-fastk.diff b/modules/nf-core/fastk/fastk/fastk-fastk.diff
deleted file mode 100644
index ac5421bd..00000000
--- a/modules/nf-core/fastk/fastk/fastk-fastk.diff
+++ /dev/null
@@ -1,542 +0,0 @@
-Changes in module 'nf-core/fastk/fastk'
---- modules/nf-core/fastk/fastk/environment.yml
-+++ modules/nf-core/fastk/fastk/environment.yml
-@@ -3,3 +3,5 @@
-   - conda-forge
-   - bioconda
-   - defaults
-+dependencies:
-+  - bioconda::false_flag # False flag to pass nf-core/lint
-
---- modules/nf-core/fastk/fastk/meta.yml
-+++ modules/nf-core/fastk/fastk/meta.yml
-@@ -9,7 +9,7 @@
-       description: "A fast K-mer counter for high-fidelity shotgun datasets"
-       homepage: "https://github.com/thegenemyers/FASTK"
-       tool_dev_url: "https://github.com/thegenemyers/FASTK"
--      licence: "https://github.com/thegenemyers/FASTK/blob/master/LICENSE"
-+      licence: ["https://github.com/thegenemyers/FASTK/blob/master/LICENSE"]
- input:
-   - meta:
-       type: map
-@@ -47,3 +47,4 @@
-   - "@mahesh-panchal"
- maintainers:
-   - "@mahesh-panchal"
-+  - "@gallvp"
-
---- modules/nf-core/fastk/fastk/main.nf
-+++ modules/nf-core/fastk/fastk/main.nf
-@@ -38,4 +38,34 @@
-         fastk: $FASTK_VERSION
-     END_VERSIONS
-     """
-+
-+    stub:
-+    // Exit if running this module with -profile conda / -profile mamba
-+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-+        error "FASTK_FASTK module does not support Conda. Please use Docker / Singularity / Podman instead."
-+    }
-+    def args = task.ext.args ?: ''
-+    def prefix = task.ext.prefix ?: "${meta.id}"
-+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-+
-+    def touch_ktab = args.contains('-t') ? "touch ${prefix}_fk.ktab .${prefix}_fk.ktab.1" : ''
-+    def touch_prof = args.contains('-p') ? "touch ${prefix}_fk.prof .${prefix}_fk.pidx.1" : ''
-+    """
-+    touch ${prefix}_fk.hist
-+    $touch_ktab
-+    $touch_prof
-+
-+    echo \\
-+    "FastK \\
-+        $args \\
-+        -T$task.cpus \\
-+        -M${task.memory.toGiga()} \\
-+        -N${prefix}_fk \\
-+        $reads"
-+
-+    cat <<-END_VERSIONS > versions.yml
-+    "${task.process}":
-+        fastk: $FASTK_VERSION
-+    END_VERSIONS
-+    """
- }
-
---- /dev/null
-+++ modules/nf-core/fastk/fastk/tests/main.nf.test.snap
-@@ -0,0 +1,346 @@
-+{
-+    "test_fastk_fastk_single_end_stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "3": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ],
-+                "hist": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "ktab": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "prof": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T19:39:28.510263"
-+    },
-+    "test_fastk_fastk_single_end": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
-+                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
-+                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
-+                        ]
-+                    ]
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
-+                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
-+                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
-+                        ]
-+                    ]
-+                ],
-+                "3": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ],
-+                "hist": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
-+                    ]
-+                ],
-+                "ktab": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
-+                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
-+                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
-+                        ]
-+                    ]
-+                ],
-+                "prof": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
-+                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
-+                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
-+                        ]
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T19:31:31.128388"
-+    },
-+    "test_fastk_fastk_paired_end": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
-+                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
-+                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
-+                        ]
-+                    ]
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
-+                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
-+                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
-+                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
-+                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
-+                        ]
-+                    ]
-+                ],
-+                "3": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ],
-+                "hist": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
-+                    ]
-+                ],
-+                "ktab": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
-+                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
-+                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
-+                        ]
-+                    ]
-+                ],
-+                "prof": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
-+                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
-+                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
-+                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
-+                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
-+                        ]
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T19:31:35.565502"
-+    },
-+    "test_fastk_fastk_paired_end_stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "3": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ],
-+                "hist": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "ktab": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "prof": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T19:39:32.570957"
-+    }
-+}
---- /dev/null
-+++ modules/nf-core/fastk/fastk/tests/tags.yml
-@@ -0,0 +1,2 @@
-+fastk/fastk:
-+  - "modules/nf-core/fastk/fastk/**"
-
---- /dev/null
-+++ modules/nf-core/fastk/fastk/tests/nextflow.config
-@@ -0,0 +1,3 @@
-+process {
-+    ext.args = '-t -p'
-+}
-
---- /dev/null
-+++ modules/nf-core/fastk/fastk/tests/main.nf.test
-@@ -0,0 +1,110 @@
-+nextflow_process {
-+
-+    name "Test Process FASTK_FASTK"
-+    script "../main.nf"
-+    config './nextflow.config'
-+    process "FASTK_FASTK"
-+
-+    tag "modules"
-+    tag "modules_nfcore"
-+    tag "fastk"
-+    tag "fastk/fastk"
-+
-+    test("test_fastk_fastk_single_end") {
-+        when {
-+            process {
-+                """
-+                input[0] = [
-+                    [ id:'test' , single_end: true ], // meta map
-+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-+                ]
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+
-+    }
-+
-+    test("test_fastk_fastk_paired_end") {
-+
-+        when {
-+            process {
-+                """
-+                input[0] = [
-+                    [ id:'test' , single_end: false ], // meta map
-+                    [
-+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-+                    ]
-+                ]
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+
-+    }
-+
-+    test("test_fastk_fastk_single_end_stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                input[0] = [
-+                    [ id:'test' , single_end: true ], // meta map
-+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-+                ]
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+
-+    }
-+
-+    test("test_fastk_fastk_paired_end_stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                input[0] = [
-+                    [ id:'test' , single_end: false ], // meta map
-+                    [
-+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-+                    ]
-+                ]
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+
-+    }
-+
-+}
-
-************************************************************
diff --git a/modules/nf-core/fastk/fastk/main.nf b/modules/nf-core/fastk/fastk/main.nf
deleted file mode 100644
index cef1a3c8..00000000
--- a/modules/nf-core/fastk/fastk/main.nf
+++ /dev/null
@@ -1,71 +0,0 @@
-process FASTK_FASTK {
-    tag "$meta.id"
-    label 'process_medium'
-
-    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.2'
-
-    input:
-    tuple val(meta), path(reads)
-
-    output:
-    tuple val(meta), path("*.hist")                      , emit: hist
-    tuple val(meta), path("*.ktab*", hidden: true)       , emit: ktab, optional: true
-    tuple val(meta), path("*.{prof,pidx}*", hidden: true), emit: prof, optional: true
-    path "versions.yml"                                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "FASTK_FASTK module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    FastK \\
-        $args \\
-        -T$task.cpus \\
-        -M${task.memory.toGiga()} \\
-        -N${prefix}_fk \\
-        $reads
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastk: $FASTK_VERSION
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "FASTK_FASTK module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-
-    def touch_ktab = args.contains('-t') ? "touch ${prefix}_fk.ktab .${prefix}_fk.ktab.1" : ''
-    def touch_prof = args.contains('-p') ? "touch ${prefix}_fk.prof .${prefix}_fk.pidx.1" : ''
-    """
-    touch ${prefix}_fk.hist
-    $touch_ktab
-    $touch_prof
-
-    echo \\
-    "FastK \\
-        $args \\
-        -T$task.cpus \\
-        -M${task.memory.toGiga()} \\
-        -N${prefix}_fk \\
-        $reads"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastk: $FASTK_VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/fastk/fastk/meta.yml b/modules/nf-core/fastk/fastk/meta.yml
deleted file mode 100644
index 3086aca5..00000000
--- a/modules/nf-core/fastk/fastk/meta.yml
+++ /dev/null
@@ -1,50 +0,0 @@
-name: "fastk_fastk"
-description: A fast K-mer counter for high-fidelity shotgun datasets
-keywords:
-  - k-mer
-  - count
-  - histogram
-tools:
-  - "fastk":
-      description: "A fast K-mer counter for high-fidelity shotgun datasets"
-      homepage: "https://github.com/thegenemyers/FASTK"
-      tool_dev_url: "https://github.com/thegenemyers/FASTK"
-      licence: ["https://github.com/thegenemyers/FASTK/blob/master/LICENSE"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - hist:
-      type: file
-      description: Histogram of k-mers
-      pattern: "*.hist"
-  - ktab:
-      type: file
-      description: A sorted table of all canonical k‑mers along with their counts.
-      pattern: "*.ktab"
-  - prof:
-      type: file
-      description: A k‑mer count profile of each sequence in the input data set.
-      pattern: "*.prof"
-authors:
-  - "@mahesh-panchal"
-maintainers:
-  - "@mahesh-panchal"
-  - "@gallvp"
diff --git a/modules/nf-core/fastk/fastk/tests/main.nf.test b/modules/nf-core/fastk/fastk/tests/main.nf.test
deleted file mode 100644
index e46d8bce..00000000
--- a/modules/nf-core/fastk/fastk/tests/main.nf.test
+++ /dev/null
@@ -1,110 +0,0 @@
-nextflow_process {
-
-    name "Test Process FASTK_FASTK"
-    script "../main.nf"
-    config './nextflow.config'
-    process "FASTK_FASTK"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "fastk"
-    tag "fastk/fastk"
-
-    test("test_fastk_fastk_single_end") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' , single_end: true ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("test_fastk_fastk_paired_end") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' , single_end: false ], // meta map
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("test_fastk_fastk_single_end_stub") {
-
-        options '-stub'
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' , single_end: true ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("test_fastk_fastk_paired_end_stub") {
-
-        options '-stub'
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' , single_end: false ], // meta map
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/fastk/fastk/tests/main.nf.test.snap b/modules/nf-core/fastk/fastk/tests/main.nf.test.snap
deleted file mode 100644
index 1e3fc4da..00000000
--- a/modules/nf-core/fastk/fastk/tests/main.nf.test.snap
+++ /dev/null
@@ -1,346 +0,0 @@
-{
-    "test_fastk_fastk_single_end_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ],
-                "hist": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "ktab": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "prof": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T19:39:28.510263"
-    },
-    "test_fastk_fastk_single_end": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
-                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
-                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
-                        ]
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
-                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
-                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
-                        ]
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ],
-                "hist": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test_fk.hist:md5,c80e12f7321e62dba4b437d7bff36ec0"
-                    ]
-                ],
-                "ktab": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,ceeacd0cb3aa69bf9b2a402830b40e26",
-                            ".test_fk.ktab.2:md5,f2629fd15b285aed3dc2d5fe546edf3f",
-                            "test_fk.ktab:md5,a605a58931a4b5029469e1c2575c8cee"
-                        ]
-                    ]
-                ],
-                "prof": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,90bc384f61d2ecdb4586ab52ab04fddf",
-                            ".test_fk.prof.1:md5,ebd48923a724cf79934f0b2ed42ba73d",
-                            "test_fk.prof:md5,43d426c95d277b8148406624d513bd40"
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T19:31:31.128388"
-    },
-    "test_fastk_fastk_paired_end": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
-                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
-                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
-                        ]
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
-                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
-                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
-                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
-                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
-                        ]
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ],
-                "hist": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_fk.hist:md5,4f75b550d87ed4f26a2b10a05ac7e98c"
-                    ]
-                ],
-                "ktab": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,7f28fb44940fda799797e3069f5d7263",
-                            ".test_fk.ktab.2:md5,c14a85c128926ace78372f09029977b1",
-                            "test_fk.ktab:md5,fddd5be0c36ad1d2131b8d8774f7657a"
-                        ]
-                    ]
-                ],
-                "prof": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,e7e760f714070a4afefb38ffff559684",
-                            ".test_fk.pidx.2:md5,a549612bbdba2506eb3311237638c4b0",
-                            ".test_fk.prof.1:md5,46a5fd9e297262b058f8c1fd062fcf56",
-                            ".test_fk.prof.2:md5,80326a7406f41ccf2e51e341fc804132",
-                            "test_fk.prof:md5,d3c7d8decd4ea6e298291b8be0e2de85"
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T19:31:35.565502"
-    },
-    "test_fastk_fastk_paired_end_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ],
-                "hist": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_fk.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "ktab": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.ktab.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.ktab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "prof": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            ".test_fk.pidx.1:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test_fk.prof:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c216a1608924d1662d2086e1de1d5abd"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T19:39:32.570957"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/fastk/fastk/tests/nextflow.config b/modules/nf-core/fastk/fastk/tests/nextflow.config
deleted file mode 100644
index c89ce9d5..00000000
--- a/modules/nf-core/fastk/fastk/tests/nextflow.config
+++ /dev/null
@@ -1,3 +0,0 @@
-process {
-    ext.args = '-t -p'
-}
diff --git a/modules/nf-core/fastk/fastk/tests/tags.yml b/modules/nf-core/fastk/fastk/tests/tags.yml
deleted file mode 100644
index 82f9df82..00000000
--- a/modules/nf-core/fastk/fastk/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-fastk/fastk:
-  - "modules/nf-core/fastk/fastk/**"
diff --git a/modules/nf-core/merqury/environment.yml b/modules/nf-core/merqury/environment.yml
new file mode 100644
index 00000000..b73f4aea
--- /dev/null
+++ b/modules/nf-core/merqury/environment.yml
@@ -0,0 +1,7 @@
+name: merqury
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::merqury=1.3
diff --git a/modules/nf-core/merqury/main.nf b/modules/nf-core/merqury/main.nf
new file mode 100644
index 00000000..196b0dfc
--- /dev/null
+++ b/modules/nf-core/merqury/main.nf
@@ -0,0 +1,60 @@
+process MERQURY {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/merqury:1.3--hdfd78af_1':
+        'biocontainers/merqury:1.3--hdfd78af_1' }"
+
+    input:
+    tuple val(meta), path(meryl_db), path(assembly)
+
+    output:
+    tuple val(meta), path("*_only.bed")          , emit: assembly_only_kmers_bed
+    tuple val(meta), path("*_only.wig")          , emit: assembly_only_kmers_wig
+    tuple val(meta), path("*.completeness.stats"), emit: stats
+    tuple val(meta), path("*.dist_only.hist")    , emit: dist_hist
+    tuple val(meta), path("*.spectra-cn.fl.png") , emit: spectra_cn_fl_png
+    tuple val(meta), path("*.spectra-cn.hist")   , emit: spectra_cn_hist
+    tuple val(meta), path("*.spectra-cn.ln.png") , emit: spectra_cn_ln_png
+    tuple val(meta), path("*.spectra-cn.st.png") , emit: spectra_cn_st_png
+    tuple val(meta), path("*.spectra-asm.fl.png"), emit: spectra_asm_fl_png
+    tuple val(meta), path("*.spectra-asm.hist")  , emit: spectra_asm_hist
+    tuple val(meta), path("*.spectra-asm.ln.png"), emit: spectra_asm_ln_png
+    tuple val(meta), path("*.spectra-asm.st.png"), emit: spectra_asm_st_png
+    tuple val(meta), path("${prefix}.qv")        , emit: assembly_qv
+    tuple val(meta), path("${prefix}.*.qv")      , emit: scaffold_qv
+    tuple val(meta), path("*.hist.ploidy")       , emit: read_ploidy
+    tuple val(meta), path("*.hapmers.blob.png")  , emit: hapmers_blob_png           , optional: true
+    path "versions.yml"                          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = 1.3
+    """
+    # Nextflow changes the container --entrypoint to /bin/bash (container default entrypoint: /usr/local/env-execute)
+    # Check for container variable initialisation script and source it.
+    if [ -f "/usr/local/env-activate.sh" ]; then
+        set +u  # Otherwise, errors out because of various unbound variables
+        . "/usr/local/env-activate.sh"
+        set -u
+    fi
+    # limit meryl to use the assigned number of cores.
+    export OMP_NUM_THREADS=$task.cpus
+
+    merqury.sh \\
+        $meryl_db \\
+        $assembly \\
+        $prefix
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        merqury: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/merqury/meta.yml b/modules/nf-core/merqury/meta.yml
new file mode 100644
index 00000000..88825386
--- /dev/null
+++ b/modules/nf-core/merqury/meta.yml
@@ -0,0 +1,98 @@
+name: "merqury"
+description: k-mer based assembly evaluation.
+keywords:
+  - "k-mer"
+  - "assembly"
+  - "evaluation"
+tools:
+  - "merqury":
+      description: "Evaluate genome assemblies with k-mers and more."
+      tool_dev_url: "https://github.com/marbl/merqury"
+      doi: "10.1186/s13059-020-02134-9"
+      licence: "['PUBLIC DOMAIN']"
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - meryl_db:
+      type: file
+      description: "Meryl read database"
+  - assembly:
+      type: file
+      description: FASTA assembly file
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - assembly_only_kmers_bed:
+      type: file
+      description: "The positions of the k-mers found only in an assembly for further investigation in .bed"
+      pattern: "*_only.bed"
+  - assembly_only_kmers_wig:
+      type: file
+      description: "The positions of the k-mers found only in an assembly for further investigation in .wig"
+      pattern: "*_only.wig"
+  - stats:
+      type: file
+      description: Assembly statistics file
+      pattern: "*.completeness.stats"
+  - dist_hist:
+      type: file
+      description: Histogram
+      pattern: "*.dist_only.hist"
+  - spectra_cn_fl_png:
+      type: file
+      description: "Unstacked copy number spectra filled plot in PNG format"
+      pattern: "*.spectra-cn.fl.png"
+  - spectra_cn_ln_png:
+      type: file
+      description: "Unstacked copy number spectra line plot in PNG format"
+      pattern: "*.spectra-cn.ln.png"
+  - spectra_cn_st_png:
+      type: file
+      description: "Stacked copy number spectra line plot in PNG format"
+      pattern: "*.spectra-cn.st.png"
+  - spectra_cn_hist:
+      type: file
+      description: "Copy number spectra histogram"
+      pattern: "*.spectra-cn.hist"
+  - spectra_asm_fl_png:
+      type: file
+      description: "Unstacked assembly spectra filled plot in PNG format"
+      pattern: "*.spectra-asm.fl.png"
+  - spectra_asm_ln_png:
+      type: file
+      description: "Unstacked assembly spectra line plot in PNG format"
+      pattern: "*.spectra-asm.ln.png"
+  - spectra_asm_st_png:
+      type: file
+      description: "Stacked assembly spectra line plot in PNG format"
+      pattern: "*.spectra-asm.st.png"
+  - spectra_asm_hist:
+      type: file
+      description: "Assembly spectra histogram"
+      pattern: "*.spectra-asm.hist"
+  - assembly_qv:
+      type: file
+      description: "Assembly consensus quality estimation"
+      pattern: "*.qv"
+  - scaffold_qv:
+      type: file
+      description: "Scaffold consensus quality estimation"
+      pattern: "*.qv"
+  - read_ploidy:
+      type: file
+      description: "Ploidy estimate from read k-mer database"
+      pattern: "*.hist.ploidy"
+authors:
+  - "@mahesh-panchal"
+maintainers:
+  - "@mahesh-panchal"
diff --git a/modules/nf-core/merquryfk/merquryfk/environment.yml b/modules/nf-core/merquryfk/merquryfk/environment.yml
deleted file mode 100644
index 325bb013..00000000
--- a/modules/nf-core/merquryfk/merquryfk/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: merquryfk_merquryfk
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::false_flag # False flag to pass nf-core/lint
diff --git a/modules/nf-core/merquryfk/merquryfk/main.nf b/modules/nf-core/merquryfk/merquryfk/main.nf
deleted file mode 100644
index ecc0f70f..00000000
--- a/modules/nf-core/merquryfk/merquryfk/main.nf
+++ /dev/null
@@ -1,101 +0,0 @@
-process MERQURYFK_MERQURYFK {
-    tag "$meta.id"
-    label 'process_medium'
-
-    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.2'
-
-    input:
-    tuple val(meta), path(fastk_hist), path(fastk_ktab), path(assembly), path(haplotigs)
-
-    output:
-    tuple val(meta), path("${prefix}.completeness.stats") , emit: stats
-    tuple val(meta), path("${prefix}.*_only.bed")         , emit: bed
-    tuple val(meta), path("${prefix}.*.qv")               , emit: assembly_qv
-    tuple val(meta), path("${prefix}.*.spectra-cn.fl.png"), emit: spectra_cn_fl_png,  optional: true
-    tuple val(meta), path("${prefix}.*.spectra-cn.fl.pdf"), emit: spectra_cn_fl_pdf,  optional: true
-    tuple val(meta), path("${prefix}.*.spectra-cn.ln.png"), emit: spectra_cn_ln_png,  optional: true
-    tuple val(meta), path("${prefix}.*.spectra-cn.ln.pdf"), emit: spectra_cn_ln_pdf,  optional: true
-    tuple val(meta), path("${prefix}.*.spectra-cn.st.png"), emit: spectra_cn_st_png,  optional: true
-    tuple val(meta), path("${prefix}.*.spectra-cn.st.pdf"), emit: spectra_cn_st_pdf,  optional: true
-    tuple val(meta), path("${prefix}.qv")                 , emit: qv
-    tuple val(meta), path("${prefix}.spectra-asm.fl.png") , emit: spectra_asm_fl_png, optional: true
-    tuple val(meta), path("${prefix}.spectra-asm.fl.pdf") , emit: spectra_asm_fl_pdf, optional: true
-    tuple val(meta), path("${prefix}.spectra-asm.ln.png") , emit: spectra_asm_ln_png, optional: true
-    tuple val(meta), path("${prefix}.spectra-asm.ln.pdf") , emit: spectra_asm_ln_pdf, optional: true
-    tuple val(meta), path("${prefix}.spectra-asm.st.png") , emit: spectra_asm_st_png, optional: true
-    tuple val(meta), path("${prefix}.spectra-asm.st.pdf") , emit: spectra_asm_st_pdf, optional: true
-    path "versions.yml"                                   , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args            = task.ext.args         ?: ''
-    prefix              = task.ext.prefix       ?: "${meta.id}"
-    def ktabs           = fastk_ktab.findAll    { it.toString().endsWith(".ktab") }.collect { "$it" }.join(' ')
-    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    MerquryFK \\
-        $args \\
-        -T$task.cpus \\
-        $ktabs \\
-        $assembly \\
-        $haplotigs \\
-        $prefix
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastk: $FASTK_VERSION
-        merquryfk: $MERQURY_VERSION
-        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args            = task.ext.args         ?: ''
-    prefix              = task.ext.prefix       ?: "${meta.id}"
-    def ktabs           = fastk_ktab.findAll    { it.toString().endsWith(".ktab") }.collect { "$it" }.join(' ')
-    def FASTK_VERSION   = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-
-    def touch_asm_bed   = "touch ${prefix}.${assembly.baseName}_only.bed"
-    def touch_asm_qv    = "touch ${prefix}.${assembly.baseName}.qv"
-    def touch_haplo_bed = haplotigs ? "touch ${prefix}.${haplotigs.baseName}_only.bed" : ''
-    def touch_haplo_qv  = haplotigs ? "touch ${prefix}.${haplotigs.baseName}.qv" : ''
-    """
-    touch "${prefix}.completeness.stats"
-
-    $touch_asm_bed
-    $touch_asm_qv
-    $touch_haplo_bed
-    $touch_haplo_qv
-
-    touch "${prefix}.qv"
-
-    echo \\
-    "MerquryFK \\
-        $args \\
-        -T$task.cpus \\
-        $ktabs \\
-        $assembly \\
-        $haplotigs \\
-        $prefix"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastk: $FASTK_VERSION
-        merquryfk: $MERQURY_VERSION
-        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff b/modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff
deleted file mode 100644
index b630c090..00000000
--- a/modules/nf-core/merquryfk/merquryfk/merquryfk-merquryfk.diff
+++ /dev/null
@@ -1,1060 +0,0 @@
-Changes in module 'nf-core/merquryfk/merquryfk'
---- modules/nf-core/merquryfk/merquryfk/environment.yml
-+++ modules/nf-core/merquryfk/merquryfk/environment.yml
-@@ -3,3 +3,5 @@
-   - conda-forge
-   - bioconda
-   - defaults
-+dependencies:
-+  - bioconda::false_flag # False flag to pass nf-core/lint
-
---- modules/nf-core/merquryfk/merquryfk/meta.yml
-+++ modules/nf-core/merquryfk/merquryfk/meta.yml
-@@ -9,7 +9,7 @@
-       description: "FastK based version of Merqury"
-       homepage: "https://github.com/thegenemyers/MERQURY.FK"
-       tool_dev_url: "https://github.com/thegenemyers/MERQURY.FK"
--      licence: "https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"
-+      licence: ["https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"]
- input:
-   - meta:
-       type: map
-@@ -110,3 +110,4 @@
-   - "@mahesh-panchal"
- maintainers:
-   - "@mahesh-panchal"
-+  - "@gallvp"
-
---- modules/nf-core/merquryfk/merquryfk/main.nf
-+++ modules/nf-core/merquryfk/merquryfk/main.nf
-@@ -55,4 +55,45 @@
-         r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
-     END_VERSIONS
-     """
-+
-+    stub:
-+    // Exit if running this module with -profile conda / -profile mamba
-+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-+        error "MERQURYFK_MERQURYFK module does not support Conda. Please use Docker / Singularity / Podman instead."
-+    }
-+    def args = task.ext.args ?: ''
-+    prefix = task.ext.prefix ?: "${meta.id}"
-+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-+    def MERQURY_VERSION = '8ae344092df5dcaf83cfb7f90f662597a9b1fc61' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-+
-+    def touch_asm_bed   = "touch ${prefix}.${assembly.baseName}_only.bed"
-+    def touch_asm_qv    = "touch ${prefix}.${assembly.baseName}.qv"
-+    def touch_haplo_bed = haplotigs ? "touch ${prefix}.${haplotigs.baseName}_only.bed" : ''
-+    def touch_haplo_qv  = haplotigs ? "touch ${prefix}.${haplotigs.baseName}.qv" : ''
-+    """
-+    touch "${prefix}.completeness.stats"
-+
-+    $touch_asm_bed
-+    $touch_asm_qv
-+    $touch_haplo_bed
-+    $touch_haplo_qv
-+
-+    touch "${prefix}.qv"
-+
-+    echo \\
-+    "MerquryFK \\
-+        $args \\
-+        -T$task.cpus \\
-+        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }} \\
-+        $assembly \\
-+        $haplotigs \\
-+        $prefix"
-+
-+    cat <<-END_VERSIONS > versions.yml
-+    "${task.process}":
-+        fastk: $FASTK_VERSION
-+        merquryfk: $MERQURY_VERSION
-+        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
-+    END_VERSIONS
-+    """
- }
-
---- /dev/null
-+++ modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
-@@ -0,0 +1,802 @@
-+{
-+    "multiple_minimal_stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "10": [
-+                    
-+                ],
-+                "11": [
-+                    
-+                ],
-+                "12": [
-+                    
-+                ],
-+                "13": [
-+                    
-+                ],
-+                "14": [
-+                    
-+                ],
-+                "15": [
-+                    
-+                ],
-+                "16": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "3": [
-+                    
-+                ],
-+                "4": [
-+                    
-+                ],
-+                "5": [
-+                    
-+                ],
-+                "6": [
-+                    
-+                ],
-+                "7": [
-+                    
-+                ],
-+                "8": [
-+                    
-+                ],
-+                "9": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "assembly_qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "bed": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        [
-+                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "spectra_asm_fl_pdf": [
-+                    
-+                ],
-+                "spectra_asm_fl_png": [
-+                    
-+                ],
-+                "spectra_asm_ln_pdf": [
-+                    
-+                ],
-+                "spectra_asm_ln_png": [
-+                    
-+                ],
-+                "spectra_asm_st_pdf": [
-+                    
-+                ],
-+                "spectra_asm_st_png": [
-+                    
-+                ],
-+                "spectra_cn_fl_pdf": [
-+                    
-+                ],
-+                "spectra_cn_fl_png": [
-+                    
-+                ],
-+                "spectra_cn_ln_pdf": [
-+                    
-+                ],
-+                "spectra_cn_ln_png": [
-+                    
-+                ],
-+                "spectra_cn_st_pdf": [
-+                    
-+                ],
-+                "spectra_cn_st_png": [
-+                    
-+                ],
-+                "stats": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T20:21:30.488412"
-+    },
-+    "test_merquryfk_merquryfk_pdf": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-+                    ]
-+                ],
-+                "10": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-+                    ]
-+                ],
-+                "11": [
-+                    
-+                ],
-+                "12": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-+                    ]
-+                ],
-+                "13": [
-+                    
-+                ],
-+                "14": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-+                    ]
-+                ],
-+                "15": [
-+                    
-+                ],
-+                "16": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-+                    ]
-+                ],
-+                "3": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "4": [
-+                    
-+                ],
-+                "5": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "6": [
-+                    
-+                ],
-+                "7": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "8": [
-+                    
-+                ],
-+                "9": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-+                    ]
-+                ],
-+                "assembly_qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-+                    ]
-+                ],
-+                "bed": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-+                    ]
-+                ],
-+                "qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-+                    ]
-+                ],
-+                "spectra_asm_fl_pdf": [
-+                    
-+                ],
-+                "spectra_asm_fl_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-+                    ]
-+                ],
-+                "spectra_asm_ln_pdf": [
-+                    
-+                ],
-+                "spectra_asm_ln_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-+                    ]
-+                ],
-+                "spectra_asm_st_pdf": [
-+                    
-+                ],
-+                "spectra_asm_st_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-+                    ]
-+                ],
-+                "spectra_cn_fl_pdf": [
-+                    
-+                ],
-+                "spectra_cn_fl_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "spectra_cn_ln_pdf": [
-+                    
-+                ],
-+                "spectra_cn_ln_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "spectra_cn_st_pdf": [
-+                    
-+                ],
-+                "spectra_cn_st_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "stats": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T19:58:05.545683"
-+    },
-+    "single_minimal_stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "10": [
-+                    
-+                ],
-+                "11": [
-+                    
-+                ],
-+                "12": [
-+                    
-+                ],
-+                "13": [
-+                    
-+                ],
-+                "14": [
-+                    
-+                ],
-+                "15": [
-+                    
-+                ],
-+                "16": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "3": [
-+                    
-+                ],
-+                "4": [
-+                    
-+                ],
-+                "5": [
-+                    
-+                ],
-+                "6": [
-+                    
-+                ],
-+                "7": [
-+                    
-+                ],
-+                "8": [
-+                    
-+                ],
-+                "9": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "assembly_qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "bed": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "spectra_asm_fl_pdf": [
-+                    
-+                ],
-+                "spectra_asm_fl_png": [
-+                    
-+                ],
-+                "spectra_asm_ln_pdf": [
-+                    
-+                ],
-+                "spectra_asm_ln_png": [
-+                    
-+                ],
-+                "spectra_asm_st_pdf": [
-+                    
-+                ],
-+                "spectra_asm_st_png": [
-+                    
-+                ],
-+                "spectra_cn_fl_pdf": [
-+                    
-+                ],
-+                "spectra_cn_fl_png": [
-+                    
-+                ],
-+                "spectra_cn_ln_pdf": [
-+                    
-+                ],
-+                "spectra_cn_ln_png": [
-+                    
-+                ],
-+                "spectra_cn_st_pdf": [
-+                    
-+                ],
-+                "spectra_cn_st_png": [
-+                    
-+                ],
-+                "stats": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T20:21:19.606389"
-+    },
-+    "test_merquryfk_merquryfk_png": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-+                    ]
-+                ],
-+                "1": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-+                    ]
-+                ],
-+                "10": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-+                    ]
-+                ],
-+                "11": [
-+                    
-+                ],
-+                "12": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-+                    ]
-+                ],
-+                "13": [
-+                    
-+                ],
-+                "14": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-+                    ]
-+                ],
-+                "15": [
-+                    
-+                ],
-+                "16": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ],
-+                "2": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-+                    ]
-+                ],
-+                "3": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "4": [
-+                    
-+                ],
-+                "5": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "6": [
-+                    
-+                ],
-+                "7": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "8": [
-+                    
-+                ],
-+                "9": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-+                    ]
-+                ],
-+                "assembly_qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-+                    ]
-+                ],
-+                "bed": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-+                    ]
-+                ],
-+                "qv": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-+                    ]
-+                ],
-+                "spectra_asm_fl_pdf": [
-+                    
-+                ],
-+                "spectra_asm_fl_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-+                    ]
-+                ],
-+                "spectra_asm_ln_pdf": [
-+                    
-+                ],
-+                "spectra_asm_ln_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-+                    ]
-+                ],
-+                "spectra_asm_st_pdf": [
-+                    
-+                ],
-+                "spectra_asm_st_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-+                    ]
-+                ],
-+                "spectra_cn_fl_pdf": [
-+                    
-+                ],
-+                "spectra_cn_fl_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "spectra_cn_ln_pdf": [
-+                    
-+                ],
-+                "spectra_cn_ln_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "spectra_cn_st_pdf": [
-+                    
-+                ],
-+                "spectra_cn_st_png": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-+                    ]
-+                ],
-+                "stats": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": true
-+                        },
-+                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-18T19:57:31.981156"
-+    }
-+}
---- /dev/null
-+++ modules/nf-core/merquryfk/merquryfk/tests/tags.yml
-@@ -0,0 +1,2 @@
-+merquryfk/merquryfk:
-+  - "modules/nf-core/merquryfk/merquryfk/**"
-
---- /dev/null
-+++ modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
-@@ -0,0 +1,11 @@
-+process {
-+    withName: 'FASTK_.*' {
-+        ext.args   = '-t'
-+    }
-+    withName: 'test_merquryfk_merquryfk_png:MERQURYFK_MERQURYFK' {
-+        ext.args   = '-lfs'
-+    }
-+    withName: 'test_merquryfk_merquryfk_pdf:MERQURYFK_MERQURYFK' {
-+        ext.args   = '-lfs -pdf'
-+    }
-+}
-
---- /dev/null
-+++ modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
-@@ -0,0 +1,153 @@
-+nextflow_process {
-+
-+    name "Test Process MERQURYFK_MERQURYFK"
-+    script "../main.nf"
-+    config "./nextflow.config"
-+    process "MERQURYFK_MERQURYFK"
-+
-+    tag "modules"
-+    tag "modules_nfcore"
-+    tag "merquryfk"
-+    tag "merquryfk/merquryfk"
-+    tag "fastk/fastk"
-+
-+    setup {
-+        run("FASTK_FASTK") {
-+            script "../../../fastk/fastk"
-+            process {
-+                """
-+                input[0] =  [
-+                    [ id:'test', single_end:true ], // meta map
-+                    file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-+                ]
-+                """
-+            }
-+        }
-+    }
-+
-+    test("test_merquryfk_merquryfk_png") {
-+
-+        when {
-+            process {
-+                """
-+                assembly = [
-+                    [ id:'test', single_end:true ], // meta map
-+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-+                ]
-+
-+                haplotigs = [
-+                    [ id:'test', single_end:true ], []
-+                ]
-+
-+                input[0] = FASTK_FASTK.out.hist
-+                            | join( FASTK_FASTK.out.ktab )
-+                            | join( Channel.value( assembly ) )
-+                            | join( Channel.value( haplotigs ) )
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
-+
-+    test("test_merquryfk_merquryfk_pdf") {
-+
-+        when {
-+            process {
-+                """
-+                assembly = [
-+                    [ id:'test', single_end:true ], // meta map
-+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-+                ]
-+
-+                haplotigs = [
-+                    [ id:'test', single_end:true ], []
-+                ]
-+
-+                input[0] = FASTK_FASTK.out.hist
-+                            | join( FASTK_FASTK.out.ktab )
-+                            | join( Channel.value( assembly ) )
-+                            | join( Channel.value( haplotigs ) )
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
-+
-+    test("single_minimal_stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                assembly = [
-+                    [ id:'test', single_end:true ], // meta map
-+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-+                ]
-+
-+                haplotigs = [
-+                    [ id:'test', single_end:true ], []
-+                ]
-+
-+                input[0] = FASTK_FASTK.out.hist
-+                            | join( FASTK_FASTK.out.ktab )
-+                            | join( Channel.value( assembly ) )
-+                            | join( Channel.value( haplotigs ) )
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
-+
-+    test("multiple_minimal_stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                assembly = [
-+                    [ id:'test', single_end:true ], // meta map
-+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-+                ]
-+
-+                haplotigs = [
-+                    [ id:'test', single_end:true ],
-+                    file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
-+                ]
-+
-+                input[0] = FASTK_FASTK.out.hist
-+                            | join( FASTK_FASTK.out.ktab )
-+                            | join( Channel.value( assembly ) )
-+                            | join( Channel.value( haplotigs ) )
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+                { assert process.success },
-+                { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
-+
-+}
-
-************************************************************
diff --git a/modules/nf-core/merquryfk/merquryfk/meta.yml b/modules/nf-core/merquryfk/merquryfk/meta.yml
deleted file mode 100644
index 0cdbcae6..00000000
--- a/modules/nf-core/merquryfk/merquryfk/meta.yml
+++ /dev/null
@@ -1,113 +0,0 @@
-name: "merquryfk_merquryfk"
-description: FastK based version of Merqury
-keywords:
-  - Merqury
-  - reference-free
-  - assembly evaluation
-tools:
-  - "merquryfk":
-      description: "FastK based version of Merqury"
-      homepage: "https://github.com/thegenemyers/MERQURY.FK"
-      tool_dev_url: "https://github.com/thegenemyers/MERQURY.FK"
-      licence: ["https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fastk_hist:
-      type: file
-      description: A histogram files from the program FastK
-      pattern: "*.hist"
-  - fastk_ktab:
-      type: file
-      description: Histogram ktab files from the program FastK (option -t)
-      pattern: "*.ktab*"
-  - assembly:
-      type: file
-      description: Genome (primary) assembly files (fasta format)
-      pattern: ".fasta"
-  - haplotigs:
-      type: file
-      description: Assembly haplotigs (fasta format)
-      pattern: ".fasta"
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - stats:
-      type: file
-      description: Assembly statistics file
-      pattern: "*.completeness.stats"
-  - bed:
-      type: file
-      description: Assembly only kmer positions not supported by reads in bed format
-      pattern: "*_only.bed"
-  - spectra_cn_fl_png:
-      type: file
-      description: "Unstacked copy number spectra filled plot in PNG format"
-      pattern: "*.spectra-cn.fl.png"
-  - spectra_cn_ln_png:
-      type: file
-      description: "Unstacked copy number spectra line plot in PNG format"
-      pattern: "*.spectra-cn.ln.png"
-  - spectra_cn_st_png:
-      type: file
-      description: "Stacked copy number spectra line plot in PNG format"
-      pattern: "*.spectra-cn.st.png"
-  - spectra_asm_fl_png:
-      type: file
-      description: "Unstacked assembly spectra filled plot in PNG format"
-      pattern: "*.spectra-asm.fl.png"
-  - spectra_asm_ln_png:
-      type: file
-      description: "Unstacked assembly spectra line plot in PNG format"
-      pattern: "*.spectra-asm.ln.png"
-  - spectra_asm_st_png:
-      type: file
-      description: "Stacked assembly spectra line plot in PNG format"
-      pattern: "*.spectra-asm.st.png"
-  - spectra_cn_fl_pdf:
-      type: file
-      description: "Unstacked copy number spectra filled plot in PDF format"
-      pattern: "*.spectra-cn.fl.pdf"
-  - spectra_cn_ln_pdf:
-      type: file
-      description: "Unstacked copy number spectra line plot in PDF format"
-      pattern: "*.spectra-cn.ln.pdf"
-  - spectra_cn_st_pdf:
-      type: file
-      description: "Stacked copy number spectra line plot in PDF format"
-      pattern: "*.spectra-cn.st.pdf"
-  - spectra_asm_fl_pdf:
-      type: file
-      description: "Unstacked assembly spectra filled plot in PDF format"
-      pattern: "*.spectra-asm.fl.pdf"
-  - spectra_asm_ln_pdf:
-      type: file
-      description: "Unstacked assembly spectra line plot in PDF format"
-      pattern: "*.spectra-asm.ln.pdf"
-  - spectra_asm_st_pdf:
-      type: file
-      description: "Stacked assembly spectra line plot in PDF format"
-      pattern: "*.spectra-asm.st.pdf"
-  - assembly_qv:
-      type: file
-      description: "error and qv table for each scaffold of the assembly"
-      pattern: "*.qv"
-  - qv:
-      type: file
-      description: "error and qv of each assembly as a whole"
-      pattern: "*.qv"
-authors:
-  - "@mahesh-panchal"
-maintainers:
-  - "@mahesh-panchal"
-  - "@gallvp"
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test b/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
deleted file mode 100644
index 5c4cce76..00000000
--- a/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test
+++ /dev/null
@@ -1,153 +0,0 @@
-nextflow_process {
-
-    name "Test Process MERQURYFK_MERQURYFK"
-    script "../main.nf"
-    config "./nextflow.config"
-    process "MERQURYFK_MERQURYFK"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "merquryfk"
-    tag "merquryfk/merquryfk"
-    tag "fastk/fastk"
-
-    setup {
-        run("FASTK_FASTK") {
-            script "../../../fastk/fastk"
-            process {
-                """
-                input[0] =  [
-                    [ id:'test', single_end:true ], // meta map
-                    file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                ]
-                """
-            }
-        }
-    }
-
-    test("test_merquryfk_merquryfk_png") {
-
-        when {
-            process {
-                """
-                assembly = [
-                    [ id:'test', single_end:true ], // meta map
-                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                ]
-
-                haplotigs = [
-                    [ id:'test', single_end:true ], []
-                ]
-
-                input[0] = FASTK_FASTK.out.hist
-                            | join( FASTK_FASTK.out.ktab )
-                            | join( Channel.value( assembly ) )
-                            | join( Channel.value( haplotigs ) )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("test_merquryfk_merquryfk_pdf") {
-
-        when {
-            process {
-                """
-                assembly = [
-                    [ id:'test', single_end:true ], // meta map
-                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                ]
-
-                haplotigs = [
-                    [ id:'test', single_end:true ], []
-                ]
-
-                input[0] = FASTK_FASTK.out.hist
-                            | join( FASTK_FASTK.out.ktab )
-                            | join( Channel.value( assembly ) )
-                            | join( Channel.value( haplotigs ) )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("single_minimal_stub") {
-
-        options '-stub'
-
-        when {
-            process {
-                """
-                assembly = [
-                    [ id:'test', single_end:true ], // meta map
-                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                ]
-
-                haplotigs = [
-                    [ id:'test', single_end:true ], []
-                ]
-
-                input[0] = FASTK_FASTK.out.hist
-                            | join( FASTK_FASTK.out.ktab )
-                            | join( Channel.value( assembly ) )
-                            | join( Channel.value( haplotigs ) )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("multiple_minimal_stub") {
-
-        options '-stub'
-
-        when {
-            process {
-                """
-                assembly = [
-                    [ id:'test', single_end:true ], // meta map
-                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                ]
-
-                haplotigs = [
-                    [ id:'test', single_end:true ],
-                    file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
-                ]
-
-                input[0] = FASTK_FASTK.out.hist
-                            | join( FASTK_FASTK.out.ktab )
-                            | join( Channel.value( assembly ) )
-                            | join( Channel.value( haplotigs ) )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-}
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap b/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
deleted file mode 100644
index eebc609f..00000000
--- a/modules/nf-core/merquryfk/merquryfk/tests/main.nf.test.snap
+++ /dev/null
@@ -1,802 +0,0 @@
-{
-    "multiple_minimal_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "10": [
-                    
-                ],
-                "11": [
-                    
-                ],
-                "12": [
-                    
-                ],
-                "13": [
-                    
-                ],
-                "14": [
-                    
-                ],
-                "15": [
-                    
-                ],
-                "16": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    
-                ],
-                "5": [
-                    
-                ],
-                "6": [
-                    
-                ],
-                "7": [
-                    
-                ],
-                "8": [
-                    
-                ],
-                "9": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "assembly_qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.genome2.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "bed": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        [
-                            "test.genome2_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "spectra_asm_fl_pdf": [
-                    
-                ],
-                "spectra_asm_fl_png": [
-                    
-                ],
-                "spectra_asm_ln_pdf": [
-                    
-                ],
-                "spectra_asm_ln_png": [
-                    
-                ],
-                "spectra_asm_st_pdf": [
-                    
-                ],
-                "spectra_asm_st_png": [
-                    
-                ],
-                "spectra_cn_fl_pdf": [
-                    
-                ],
-                "spectra_cn_fl_png": [
-                    
-                ],
-                "spectra_cn_ln_pdf": [
-                    
-                ],
-                "spectra_cn_ln_png": [
-                    
-                ],
-                "spectra_cn_st_pdf": [
-                    
-                ],
-                "spectra_cn_st_png": [
-                    
-                ],
-                "stats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T20:21:30.488412"
-    },
-    "test_merquryfk_merquryfk_pdf": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-                    ]
-                ],
-                "10": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-                    ]
-                ],
-                "11": [
-                    
-                ],
-                "12": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-                    ]
-                ],
-                "13": [
-                    
-                ],
-                "14": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-                    ]
-                ],
-                "15": [
-                    
-                ],
-                "16": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "4": [
-                    
-                ],
-                "5": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "6": [
-                    
-                ],
-                "7": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "8": [
-                    
-                ],
-                "9": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-                    ]
-                ],
-                "assembly_qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-                    ]
-                ],
-                "bed": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-                    ]
-                ],
-                "qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-                    ]
-                ],
-                "spectra_asm_fl_pdf": [
-                    
-                ],
-                "spectra_asm_fl_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-                    ]
-                ],
-                "spectra_asm_ln_pdf": [
-                    
-                ],
-                "spectra_asm_ln_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-                    ]
-                ],
-                "spectra_asm_st_pdf": [
-                    
-                ],
-                "spectra_asm_st_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-                    ]
-                ],
-                "spectra_cn_fl_pdf": [
-                    
-                ],
-                "spectra_cn_fl_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "spectra_cn_ln_pdf": [
-                    
-                ],
-                "spectra_cn_ln_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "spectra_cn_st_pdf": [
-                    
-                ],
-                "spectra_cn_st_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "stats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T19:58:05.545683"
-    },
-    "single_minimal_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "10": [
-                    
-                ],
-                "11": [
-                    
-                ],
-                "12": [
-                    
-                ],
-                "13": [
-                    
-                ],
-                "14": [
-                    
-                ],
-                "15": [
-                    
-                ],
-                "16": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    
-                ],
-                "5": [
-                    
-                ],
-                "6": [
-                    
-                ],
-                "7": [
-                    
-                ],
-                "8": [
-                    
-                ],
-                "9": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "assembly_qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "bed": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "spectra_asm_fl_pdf": [
-                    
-                ],
-                "spectra_asm_fl_png": [
-                    
-                ],
-                "spectra_asm_ln_pdf": [
-                    
-                ],
-                "spectra_asm_ln_png": [
-                    
-                ],
-                "spectra_asm_st_pdf": [
-                    
-                ],
-                "spectra_asm_st_png": [
-                    
-                ],
-                "spectra_cn_fl_pdf": [
-                    
-                ],
-                "spectra_cn_fl_png": [
-                    
-                ],
-                "spectra_cn_ln_pdf": [
-                    
-                ],
-                "spectra_cn_ln_png": [
-                    
-                ],
-                "spectra_cn_st_pdf": [
-                    
-                ],
-                "spectra_cn_st_png": [
-                    
-                ],
-                "stats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T20:21:19.606389"
-    },
-    "test_merquryfk_merquryfk_png": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-                    ]
-                ],
-                "10": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-                    ]
-                ],
-                "11": [
-                    
-                ],
-                "12": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-                    ]
-                ],
-                "13": [
-                    
-                ],
-                "14": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-                    ]
-                ],
-                "15": [
-                    
-                ],
-                "16": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "4": [
-                    
-                ],
-                "5": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "6": [
-                    
-                ],
-                "7": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "8": [
-                    
-                ],
-                "9": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-                    ]
-                ],
-                "assembly_qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.qv:md5,1e11be8abfa2230024042832f58f96f9"
-                    ]
-                ],
-                "bed": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome_only.bed:md5,662d73e8a0019708feb538ec53c220f7"
-                    ]
-                ],
-                "qv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.qv:md5,02b4d5c639ca706ff707bad89e29d90e"
-                    ]
-                ],
-                "spectra_asm_fl_pdf": [
-                    
-                ],
-                "spectra_asm_fl_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.fl.png:md5,bb9ac38fe8991fbacbe791e562626d89"
-                    ]
-                ],
-                "spectra_asm_ln_pdf": [
-                    
-                ],
-                "spectra_asm_ln_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.ln.png:md5,48534a9d5e2ce9365eb1f0397c76f337"
-                    ]
-                ],
-                "spectra_asm_st_pdf": [
-                    
-                ],
-                "spectra_asm_st_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.spectra-asm.st.png:md5,5e031a8b9ba2800e291721a11f4d9c00"
-                    ]
-                ],
-                "spectra_cn_fl_pdf": [
-                    
-                ],
-                "spectra_cn_fl_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.fl.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "spectra_cn_ln_pdf": [
-                    
-                ],
-                "spectra_cn_ln_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.ln.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "spectra_cn_st_pdf": [
-                    
-                ],
-                "spectra_cn_st_png": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.genome.spectra-cn.st.png:md5,50bad6b85b8f80b8411b50e4119bc959"
-                    ]
-                ],
-                "stats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.completeness.stats:md5,797224fa75606bbda7f62caae7c9151c"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c28c863a18f7ad02798270acd2e6aa48"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-05-18T19:57:31.981156"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/nextflow.config b/modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
deleted file mode 100644
index 63d72d24..00000000
--- a/modules/nf-core/merquryfk/merquryfk/tests/nextflow.config
+++ /dev/null
@@ -1,11 +0,0 @@
-process {
-    withName: 'FASTK_.*' {
-        ext.args   = '-t'
-    }
-    withName: 'test_merquryfk_merquryfk_png:MERQURYFK_MERQURYFK' {
-        ext.args   = '-lfs'
-    }
-    withName: 'test_merquryfk_merquryfk_pdf:MERQURYFK_MERQURYFK' {
-        ext.args   = '-lfs -pdf'
-    }
-}
diff --git a/modules/nf-core/merquryfk/merquryfk/tests/tags.yml b/modules/nf-core/merquryfk/merquryfk/tests/tags.yml
deleted file mode 100644
index 7dcac99b..00000000
--- a/modules/nf-core/merquryfk/merquryfk/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-merquryfk/merquryfk:
-  - "modules/nf-core/merquryfk/merquryfk/**"
diff --git a/modules/nf-core/meryl/count/environment.yml b/modules/nf-core/meryl/count/environment.yml
new file mode 100644
index 00000000..808565ee
--- /dev/null
+++ b/modules/nf-core/meryl/count/environment.yml
@@ -0,0 +1,7 @@
+name: meryl_count
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::meryl=1.4.1
diff --git a/modules/nf-core/meryl/count/main.nf b/modules/nf-core/meryl/count/main.nf
new file mode 100644
index 00000000..2f0518f3
--- /dev/null
+++ b/modules/nf-core/meryl/count/main.nf
@@ -0,0 +1,53 @@
+process MERYL_COUNT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/meryl:1.4.1--h4ac6f70_0':
+        'biocontainers/meryl:1.4.1--h4ac6f70_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    val kvalue
+
+    output:
+    tuple val(meta), path("*.meryldb"), emit: meryl_db
+    path "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    for READ in $reads; do
+        meryl count \\
+            k=$kvalue \\
+            threads=$task.cpus \\
+            $args \\
+            $reads \\
+            output read.\${READ%.f*}.meryldb
+    done
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        meryl: \$( meryl --version |& sed 's/meryl //' )
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    for READ in $reads; do
+        touch read.\${READ%.f*}.meryldb
+    done
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        meryl: \$( meryl --version |& sed 's/meryl //' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/meryl/count/meta.yml b/modules/nf-core/meryl/count/meta.yml
new file mode 100644
index 00000000..c9347c0c
--- /dev/null
+++ b/modules/nf-core/meryl/count/meta.yml
@@ -0,0 +1,45 @@
+name: "meryl_count"
+description: A genomic k-mer counter (and sequence utility) with nice features.
+keywords:
+  - k-mer
+  - count
+  - reference-free
+tools:
+  - "meryl":
+      description: "A genomic k-mer counter (and sequence utility) with nice features. "
+      homepage: "https://github.com/marbl/meryl"
+      documentation: "https://meryl.readthedocs.io/en/latest/quick-start.html"
+      tool_dev_url: "https://github.com/marbl/meryl"
+      licence: ["GPL"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+  - kvalue:
+      type: integer
+      description: An integer value of k to use as the k-mer value.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - meryl_db:
+      type: directory
+      description: A Meryl k-mer database
+      pattern: "*.meryldb"
+authors:
+  - "@mahesh-panchal"
+maintainers:
+  - "@mahesh-panchal"
diff --git a/modules/nf-core/meryl/count/tests/main.nf.test b/modules/nf-core/meryl/count/tests/main.nf.test
new file mode 100644
index 00000000..cce46c36
--- /dev/null
+++ b/modules/nf-core/meryl/count/tests/main.nf.test
@@ -0,0 +1,60 @@
+nextflow_process {
+
+    name "Test Process MERYL_COUNT"
+    script "../main.nf"
+    process "MERYL_COUNT"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "meryl"
+    tag "meryl/count"
+
+    test("bacteroides_fragilis - fastq") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.value([
+                    [ id: 'test', single_end: true ], // meta map
+                    file( params.modules_testdata_base_path + "/genomics/prokaryotes/bacteroides_fragilis/illumina/fastq/test1_1.fastq.gz", checkIfExists: true )
+                ])
+                input[1] = Channel.value(21)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("bacteroides_fragilis - fastq - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.value([
+                    [ id: 'test', single_end: true ], // meta map
+                    file( params.modules_testdata_base_path + "/genomics/prokaryotes/bacteroides_fragilis/illumina/fastq/test1_1.fastq.gz", checkIfExists: true )
+                ])
+                input[1] = Channel.value(21)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/meryl/count/tests/main.nf.test.snap b/modules/nf-core/meryl/count/tests/main.nf.test.snap
new file mode 100644
index 00000000..da7c9f31
--- /dev/null
+++ b/modules/nf-core/meryl/count/tests/main.nf.test.snap
@@ -0,0 +1,332 @@
+{
+    "bacteroides_fragilis - fastq": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "0x000000.merylData:md5,eefafbacbd0c41e4e4851e0e79a665a2",
+                            "0x000000.merylIndex:md5,be9c1da85afee20689c5c7780b6c4996",
+                            "0x000001.merylData:md5,d7e46736fc2896085b4610c50ec74835",
+                            "0x000001.merylIndex:md5,a68e8b877ed5ce04e6f36a279b2fac0b",
+                            "0x000010.merylData:md5,635badb59ad796363c2ff31801ab3d07",
+                            "0x000010.merylIndex:md5,afed85194fcbd762b7acf86427b41cdd",
+                            "0x000011.merylData:md5,b79970fce39d47cda1312080cac2f5d4",
+                            "0x000011.merylIndex:md5,402ba5cf71656e2d19e584e4b65cadaa",
+                            "0x000100.merylData:md5,48a7160c93fe5dd22fcdf8beb30e9256",
+                            "0x000100.merylIndex:md5,fbfae07482bed8b00c6a690850c632fc",
+                            "0x000101.merylData:md5,3ff336b4d59850f6726a4bd5670d49be",
+                            "0x000101.merylIndex:md5,817edfe6dcbb07801bb9de54e0e1839a",
+                            "0x000110.merylData:md5,41f253dc8240dd26cd8bbe379968aa1f",
+                            "0x000110.merylIndex:md5,64f1400897c3108d5bc4957bea80569c",
+                            "0x000111.merylData:md5,0860dd1bb6ee8e99a315c976b0c6226a",
+                            "0x000111.merylIndex:md5,794aadc07e62b0d872763f2b40615627",
+                            "0x001000.merylData:md5,daee6b4d18a43ce3a6c46ee2e8105a93",
+                            "0x001000.merylIndex:md5,5ec6234ffd7d0380db3adc5aa3a3bb85",
+                            "0x001001.merylData:md5,7e9b6d3c6f116fe418c4a4b0529a795e",
+                            "0x001001.merylIndex:md5,eb63cc438cac77169a527bd6607bd781",
+                            "0x001010.merylData:md5,f93c20dbdd16be37cdcfa263fe3c4ca4",
+                            "0x001010.merylIndex:md5,d00fe5bd25b49bc9084b705017ccf4b9",
+                            "0x001011.merylData:md5,eb25d445533ba345ea4e315750dd878c",
+                            "0x001011.merylIndex:md5,1cacb579a9991dc5ebeef884347b81f5",
+                            "0x001100.merylData:md5,7c783f255d155c23218e14c390b4c505",
+                            "0x001100.merylIndex:md5,41a4d42d07928f3a33fd6c2d4ff613d8",
+                            "0x001101.merylData:md5,98f323f13f99a2c75ccf8db9179f4b7d",
+                            "0x001101.merylIndex:md5,528f3cdc0cbde3f0c91f8e8acd00a3e1",
+                            "0x001110.merylData:md5,12f1c899ed2878d60c3a7ee5eb599839",
+                            "0x001110.merylIndex:md5,755fc26bb9c8fec041798958f83b37b8",
+                            "0x001111.merylData:md5,594881e83a5ece0879e79e2be82847f9",
+                            "0x001111.merylIndex:md5,62625db2836f07303c9e4d383574143f",
+                            "0x010000.merylData:md5,537d1a60554bd68386509fc3edd5e609",
+                            "0x010000.merylIndex:md5,2bf23b6a552306dab61d0da0ad0789e0",
+                            "0x010001.merylData:md5,9baef5a2ea4ed43caf18cabcdccaa3eb",
+                            "0x010001.merylIndex:md5,ff98a6e5d18ab52795c6af00f47fc355",
+                            "0x010010.merylData:md5,3b37668e9f9e1fc220e1fed36ecb7f86",
+                            "0x010010.merylIndex:md5,0724a75055d62586f95f8b5c58688dd3",
+                            "0x010011.merylData:md5,b4a039219f34df414048a023beea583b",
+                            "0x010011.merylIndex:md5,34b29bdb6676e172e80202d36a0551d7",
+                            "0x010100.merylData:md5,a49dfdebd8c4b3ff5ed1f4f84128b7e7",
+                            "0x010100.merylIndex:md5,7cf00a46160bcb04de47e8bd05e8696b",
+                            "0x010101.merylData:md5,0065195381f0a8629426139f3ba97327",
+                            "0x010101.merylIndex:md5,de73cfc31abb763a11e063711f4b86bd",
+                            "0x010110.merylData:md5,b331a0a2a59e46741366b8f811eaafcb",
+                            "0x010110.merylIndex:md5,1caca7c16ffcc8070fbb58f4c9936089",
+                            "0x010111.merylData:md5,7a51306a008a7f7639d98b4d1f24be92",
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+                            "0x111110.merylIndex:md5,0cd1b81c64fee3bf067ce5ca1755a2bc",
+                            "0x111111.merylData:md5,68feb8f1bade7885a9a696bb0825a17f",
+                            "0x111111.merylIndex:md5,fd631e33829939cd7f06581c80791dc4",
+                            "merylIndex:md5,d9e794e14b1eeb0eaa4d98e10eb571cc"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a4a6baac7481f1d4595ba54622bdf33d"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-26T19:30:03.054214308"
+    },
+    "bacteroides_fragilis - fastq - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,a4a6baac7481f1d4595ba54622bdf33d"
+                ],
+                "meryl_db": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a4a6baac7481f1d4595ba54622bdf33d"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-26T19:30:13.745468405"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/meryl/count/tests/tags.yml b/modules/nf-core/meryl/count/tests/tags.yml
new file mode 100644
index 00000000..b25bfa60
--- /dev/null
+++ b/modules/nf-core/meryl/count/tests/tags.yml
@@ -0,0 +1,2 @@
+meryl/count:
+  - "modules/nf-core/meryl/count/**"
diff --git a/modules/nf-core/meryl/unionsum/environment.yml b/modules/nf-core/meryl/unionsum/environment.yml
new file mode 100644
index 00000000..b8bdcbe8
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/environment.yml
@@ -0,0 +1,7 @@
+name: meryl_unionsum
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::meryl=1.4.1
diff --git a/modules/nf-core/meryl/unionsum/main.nf b/modules/nf-core/meryl/unionsum/main.nf
new file mode 100644
index 00000000..9800f605
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/main.nf
@@ -0,0 +1,49 @@
+process MERYL_UNIONSUM {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/meryl:1.4.1--h4ac6f70_0':
+        'biocontainers/meryl:1.4.1--h4ac6f70_0' }"
+
+    input:
+    tuple val(meta), path(meryl_dbs)
+    val kvalue
+
+    output:
+    tuple val(meta), path("*.unionsum.meryl")   , emit: meryl_db
+    path "versions.yml"                         , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    meryl union-sum \\
+        k=$kvalue \\
+        threads=$task.cpus \\
+        $args \\
+        output ${prefix}.unionsum.meryl \\
+        $meryl_dbs
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        meryl: \$( meryl --version |& sed 's/meryl //' )
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.unionsum.meryl
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        meryl: \$( meryl --version |& sed 's/meryl //' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/meryl/unionsum/meta.yml b/modules/nf-core/meryl/unionsum/meta.yml
new file mode 100644
index 00000000..3bb01250
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/meta.yml
@@ -0,0 +1,43 @@
+name: "meryl_unionsum"
+description: A genomic k-mer counter (and sequence utility) with nice features.
+keywords:
+  - k-mer
+  - unionsum
+  - reference-free
+tools:
+  - "meryl":
+      description: "A genomic k-mer counter (and sequence utility) with nice features. "
+      homepage: "https://github.com/marbl/meryl"
+      documentation: "https://meryl.readthedocs.io/en/latest/quick-start.html"
+      tool_dev_url: "https://github.com/marbl/meryl"
+      licence: ["GPL"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - meryl_dbs:
+      type: directory
+      description: Meryl k-mer databases
+  - kvalue:
+      type: integer
+      description: An integer value of k to use as the k-mer value.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - meryl_db:
+      type: directory
+      description: A Meryl k-mer database that is the union sum of the input databases
+      pattern: "*.unionsum.meryldb"
+authors:
+  - "@mahesh-panchal"
+maintainers:
+  - "@mahesh-panchal"
diff --git a/modules/nf-core/meryl/unionsum/tests/main.nf.test b/modules/nf-core/meryl/unionsum/tests/main.nf.test
new file mode 100644
index 00000000..dc1bf8af
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/tests/main.nf.test
@@ -0,0 +1,123 @@
+nextflow_process {
+
+    name "Test Process MERYL_UNIONSUM"
+    script "../main.nf"
+    process "MERYL_UNIONSUM"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "meryl"
+    tag "meryl/unionsum"
+    tag "meryl/count"
+
+    test("sarscov2 - fastq - single_end") {
+
+        setup {
+            run('MERYL_COUNT'){
+                script "../../../../../modules/nf-core/meryl/count/main.nf"
+                process {
+                    """
+                    input[0] = Channel.value([
+                        [ id: 'test', single_end: true ], // meta map
+                        file( params.modules_testdata_base_path + "/genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true )
+                    ])
+                    input[1] = Channel.value(21)
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = MERYL_COUNT.out.meryl_db
+                input[1] = Channel.value(21)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq - paired_end") {
+
+        setup {
+            run('MERYL_COUNT'){
+                script "../../../../../modules/nf-core/meryl/count/main.nf"
+                process {
+                    """
+                    input[0] = Channel.value([
+                        [ id: 'test', single_end: false ], // meta map
+                        [
+                            file( params.modules_testdata_base_path + "/genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true ),
+                            file( params.modules_testdata_base_path + "/genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true )
+                        ]
+                    ])
+                    input[1] = Channel.value(21)
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = MERYL_COUNT.out.meryl_db
+                input[1] = Channel.value(21)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq- stub") {
+
+        options "-stub"
+
+        setup {
+            run('MERYL_COUNT'){
+                script "../../../../../modules/nf-core/meryl/count/main.nf"
+                process {
+                    """
+                    input[0] = Channel.value([
+                        [ id: 'test', single_end: true ], // meta map
+                        file( params.modules_testdata_base_path + "/genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true )
+                    ])
+                    input[1] = Channel.value(21)
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = MERYL_COUNT.out.meryl_db
+                input[1] = Channel.value(21)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap b/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
new file mode 100644
index 00000000..522a52c9
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
@@ -0,0 +1,627 @@
+{
+    "sarscov2 - fastq- stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,c97980ac5ebd37a77768c105861ad719"
+                ],
+                "meryl_db": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c97980ac5ebd37a77768c105861ad719"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-27T10:19:52.291287389"
+    },
+    "sarscov2 - fastq - single_end": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "0x000000.merylData:md5,7d95ca25a2d39a0b8fb5f71a77d7bddd",
+                            "0x000000.merylIndex:md5,ca9b05f5b90ed432aa1134378a86f166",
+                            "0x000001.merylData:md5,bb4e2ec7d45a2d4fa36e802b0e312965",
+                            "0x000001.merylIndex:md5,26b3ad6909cec2061c476201a0ac04cd",
+                            "0x000010.merylData:md5,b4b8e7a742b4634ca82d2d19928cacd6",
+                            "0x000010.merylIndex:md5,a266e782d80833c8e685a42f07937fc7",
+                            "0x000011.merylData:md5,bc181b55548c6d7832b65be4bed8ec56",
+                            "0x000011.merylIndex:md5,f2bb128f3da304408c81cefb0d4de879",
+                            "0x000100.merylData:md5,07da07cdc83420487641f7201c1593c2",
+                            "0x000100.merylIndex:md5,f40d2c9ecf5980328cfd766b9d90019d",
+                            "0x000101.merylData:md5,2410ff0d6604044e9175feb668e747ac",
+                            "0x000101.merylIndex:md5,f61473e3e39e6990c30bfa53e2a63302",
+                            "0x000110.merylData:md5,05804f66393bef8edf69143a6cd984bc",
+                            "0x000110.merylIndex:md5,15b6e88e01e1b1abc29b54128067a938",
+                            "0x000111.merylData:md5,a3ced1867214878de2d83dda7e805308",
+                            "0x000111.merylIndex:md5,2998f056a5f859229229b0051cb792ec",
+                            "0x001000.merylData:md5,531c743e9b5df804297fba37e5cd4a63",
+                            "0x001000.merylIndex:md5,30ab89ede28b623711675a45751b2ebc",
+                            "0x001001.merylData:md5,4303314fa9d7bb8326938537c27d381e",
+                            "0x001001.merylIndex:md5,1356eb83aa75fce8693e974bf54200d1",
+                            "0x001010.merylData:md5,77918beac0dc29069365d9bf9104237b",
+                            "0x001010.merylIndex:md5,d7db72b42185070da78104b356f4b5a1",
+                            "0x001011.merylData:md5,d7837930447de7651dee47c00a1d2afe",
+                            "0x001011.merylIndex:md5,ac9c6c4aab6172d73134d2dfc5215832",
+                            "0x001100.merylData:md5,3e5e6301e1287e7ada42e5bafa24b74f",
+                            "0x001100.merylIndex:md5,0e0736dfaa2974d7f09744ece53b3e69",
+                            "0x001101.merylData:md5,38b8f1b7982de9c7add7db0071c28d24",
+                            "0x001101.merylIndex:md5,c85afc65db43592a006e317c2e4891e8",
+                            "0x001110.merylData:md5,f189f910062d272065a0f444789e1d68",
+                            "0x001110.merylIndex:md5,701d7a951ee2bf6520845262dbd65559",
+                            "0x001111.merylData:md5,0200cf0723778bcc48cffe7bfa48137c",
+                            "0x001111.merylIndex:md5,2d445869fecabc6414242cd68a6edda5",
+                            "0x010000.merylData:md5,a1d5479d3e8bd671368c6432867a2e95",
+                            "0x010000.merylIndex:md5,ad664f2ea294871da909156a88b0dd3a",
+                            "0x010001.merylData:md5,28919fad6317bbd6fe9809665437f9ae",
+                            "0x010001.merylIndex:md5,64d482b1ef40e3de9d972f4134cb6d07",
+                            "0x010010.merylData:md5,5b4630208c973815be52221c91777f77",
+                            "0x010010.merylIndex:md5,0460424759df28005d71ebdef60e9ddf",
+                            "0x010011.merylData:md5,fc58468b0b010469b11fe3b00c771067",
+                            "0x010011.merylIndex:md5,7bd12cb4685bd7dc1aeb619e87bb3115",
+                            "0x010100.merylData:md5,0db951a9cdf9c78bea428715ddf75bb0",
+                            "0x010100.merylIndex:md5,b13f442b8fec07bf23ef751f2d94f9cb",
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+                            "0x011001.merylIndex:md5,0b510bcd0b9fbbdf5d46bea2c4735126",
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+                            "0x011010.merylIndex:md5,3eebe66a116b1d121f918899a7fdbb28",
+                            "0x011011.merylData:md5,64b7020da74d0d6a2b329e094cec17ed",
+                            "0x011011.merylIndex:md5,9e3cc561c44b83131f76344567c4d943",
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+                            "0x011100.merylIndex:md5,c796d799243b1622a38455dc274ddbff",
+                            "0x011101.merylData:md5,398aed1de15abe0855b22cc566a81065",
+                            "0x011101.merylIndex:md5,56fa2957c0c5c9b23434ce1d2070fd40",
+                            "0x011110.merylData:md5,24eb8aaa7b58da7626042435a2ee8061",
+                            "0x011110.merylIndex:md5,ba35223b3ac43b389413c605bf035a6d",
+                            "0x011111.merylData:md5,90e7bcb05dcf23278421ce8a02d4deed",
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+                            "0x100001.merylIndex:md5,49e5e583346b4491b1586c86213118f0",
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+                            "0x100110.merylIndex:md5,873ca65e4ef4d432fc356a85beaa4063",
+                            "0x100111.merylData:md5,fe791c9598de38dfa75496558a8b2f28",
+                            "0x100111.merylIndex:md5,369c608a46fe76cbc77715d163153bfb",
+                            "0x101000.merylData:md5,d89e7ae06de81df678021213e9808b4b",
+                            "0x101000.merylIndex:md5,43160d471884a484f3862750d44c2788",
+                            "0x101001.merylData:md5,8cd9b88142c08c412371e9ef66d59ef9",
+                            "0x101001.merylIndex:md5,6a5650d1c9793eeb2781fb9e57b14784",
+                            "0x101010.merylData:md5,0f17f7e3dfe8c2ec5c469597c5e66134",
+                            "0x101010.merylIndex:md5,fa2913d514e183a3c4cba31cc422dff4",
+                            "0x101011.merylData:md5,f8ab9821eebfdedabb61a106abdce55c",
+                            "0x101011.merylIndex:md5,8a5a07b1d07b0b7d71943c0980c0755e",
+                            "0x101100.merylData:md5,6a969c16cc7a522ebb1f0be5fc81056e",
+                            "0x101100.merylIndex:md5,5f12c2413a9ddca0751e4b2034ebdc87",
+                            "0x101101.merylData:md5,9cc1443daf53adcf2d95c4fd0f8d49de",
+                            "0x101101.merylIndex:md5,19dcebf34036938fed8984d8e100b579",
+                            "0x101110.merylData:md5,f0d63a933d58c9c5f0bd5885adfa0dfe",
+                            "0x101110.merylIndex:md5,d6517f33c2c4667b5c53f8b099a1f8fe",
+                            "0x101111.merylData:md5,87706ddcd8927988661d8e64e69c0723",
+                            "0x101111.merylIndex:md5,fc9671ff0358c2350a650ac1763a3368",
+                            "0x110000.merylData:md5,63ce769d2987235e28d567b3b64585c1",
+                            "0x110000.merylIndex:md5,6f7997e8622eeb89fdbd774d8cd98ba2",
+                            "0x110001.merylData:md5,0d3d10723737ac2d1037e1fe576acb22",
+                            "0x110001.merylIndex:md5,f27c0d1169639ae9e081b39afc3757f5",
+                            "0x110010.merylData:md5,3b92d7921670a6c4a4394c84286a3837",
+                            "0x110010.merylIndex:md5,ff12c1af64828ca8d054acea6ebe3440",
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+                            "0x110011.merylIndex:md5,9e5f773d3e6472581bb72f3185d4dc16",
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+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-27T10:19:40.09228319"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/meryl/unionsum/tests/tags.yml b/modules/nf-core/meryl/unionsum/tests/tags.yml
new file mode 100644
index 00000000..707dd781
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/tests/tags.yml
@@ -0,0 +1,2 @@
+meryl/unionsum:
+  - "modules/nf-core/meryl/unionsum/**"
diff --git a/modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff b/modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff
deleted file mode 100644
index d71c09b1..00000000
--- a/modules/nf-core/sratools/fasterqdump/sratools-fasterqdump.diff
+++ /dev/null
@@ -1,252 +0,0 @@
-Changes in module 'nf-core/sratools/fasterqdump'
---- modules/nf-core/sratools/fasterqdump/meta.yml
-+++ modules/nf-core/sratools/fasterqdump/meta.yml
-@@ -51,3 +51,4 @@
-   - "@Midnighter"
- maintainers:
-   - "@Midnighter"
-+  - "@gallvp"
-
---- modules/nf-core/sratools/fasterqdump/main.nf
-+++ modules/nf-core/sratools/fasterqdump/main.nf
-@@ -23,7 +23,10 @@
-     def args = task.ext.args ?: ''
-     def args2 = task.ext.args2 ?: ''
-     def prefix = task.ext.prefix ?: "${meta.id}"
--    def outfile = meta.single_end ? "${prefix}.fastq" : prefix
-+    def outfile = "${prefix}.fastq"
-+    def exclude_third = meta.single_end ? '' : "mv $outfile $prefix || echo 'No third file'"
-+    // Excludes the "${prefix}.fastq" file from output `reads` channel for paired end cases and
-+    // avoids the '.' in the path bug: https://github.com/ncbi/sra-tools/issues/865
-     def key_file = ''
-     if (certificate.toString().endsWith('.jwt')) {
-         key_file += " --perm ${certificate}"
-@@ -40,6 +43,51 @@
-         ${key_file} \\
-         ${sra}
- 
-+    $exclude_third
-+
-+    pigz \\
-+        $args2 \\
-+        --no-name \\
-+        --processes $task.cpus \\
-+        *.fastq
-+
-+    cat <<-END_VERSIONS > versions.yml
-+    "${task.process}":
-+        sratools: \$(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
-+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-+    END_VERSIONS
-+    """
-+
-+    stub:
-+    def args = task.ext.args ?: ''
-+    def args2 = task.ext.args2 ?: ''
-+    def prefix = task.ext.prefix ?: "${meta.id}"
-+    def outfile = "${prefix}.fastq"
-+    def exclude_third = meta.single_end ? '' : "mv $outfile $prefix || echo 'No third file'"
-+    // Excludes the "${prefix}.fastq" file from output `reads` channel for paired end cases and
-+    // avoids the '.' in the path bug: https://github.com/ncbi/sra-tools/issues/865
-+    def key_file = ''
-+    if (certificate.toString().endsWith('.jwt')) {
-+        key_file += " --perm ${certificate}"
-+    } else if (certificate.toString().endsWith('.ngc')) {
-+        key_file += " --ngc ${certificate}"
-+    }
-+    def touch_outfiles = meta.single_end ? "${prefix}.fastq" : "${prefix}_1.fastq ${prefix}_2.fastq"
-+    """
-+    touch $touch_outfiles
-+
-+    export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
-+
-+    echo \\
-+    "fasterq-dump \\
-+        $args \\
-+        --threads $task.cpus \\
-+        --outfile $outfile \\
-+        ${key_file} \\
-+        ${sra}"
-+
-+    $exclude_third
-+
-     pigz \\
-         $args2 \\
-         --no-name \\
-
---- modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
-+++ modules/nf-core/sratools/fasterqdump/tests/main.nf.test.snap
-@@ -30,9 +30,44 @@
-         ],
-         "meta": {
-             "nf-test": "0.8.4",
--            "nextflow": "24.01.0"
-+            "nextflow": "23.10.1"
-         },
--        "timestamp": "2024-02-28T15:25:52.837288"
-+        "timestamp": "2024-05-17T22:01:43.486256"
-+    },
-+    "Single-end-stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test_single_end",
-+                            "single_end": true
-+                        },
-+                        "test_single_end.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "1": [
-+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
-+                ],
-+                "reads": [
-+                    [
-+                        {
-+                            "id": "test_single_end",
-+                            "single_end": true
-+                        },
-+                        "test_single_end.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-17T21:40:42.335786"
-     },
-     "Paired-end": {
-         "content": [
-@@ -71,8 +106,49 @@
-         ],
-         "meta": {
-             "nf-test": "0.8.4",
--            "nextflow": "24.01.0"
-+            "nextflow": "23.10.1"
-         },
--        "timestamp": "2024-02-28T15:26:42.466223"
-+        "timestamp": "2024-05-17T22:09:55.6396"
-+    },
-+    "Paired-end-stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test_paired_end",
-+                            "single_end": false
-+                        },
-+                        [
-+                            "test_paired_end_1.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_paired_end_2.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "1": [
-+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
-+                ],
-+                "reads": [
-+                    [
-+                        {
-+                            "id": "test_paired_end",
-+                            "single_end": false
-+                        },
-+                        [
-+                            "test_paired_end_1.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-+                            "test_paired_end_2.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,a3d61a9761e1606ef8459f0b68821d7a"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-17T20:35:44.782058"
-     }
- }
---- modules/nf-core/sratools/fasterqdump/tests/main.nf.test
-+++ modules/nf-core/sratools/fasterqdump/tests/main.nf.test
-@@ -1,7 +1,6 @@
- nextflow_process {
-     name "Test Process SRATOOLS_FASTERQDUMP"
-     script "../main.nf"
--    config "./nextflow.config"
-     process "SRATOOLS_FASTERQDUMP"
-     tag "modules"
-     tag "modules_nfcore"
-@@ -70,4 +69,54 @@
-             )
-         }
-     }
-+
-+    test("Single-end-stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                input[0] = [
-+                    [ id:'test_single_end', single_end:true ],
-+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR13255544.tar.gz', checkIfExists: true)
-+                ]
-+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
-+                input[2] = []
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+            { assert process.success },
-+            { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
-+
-+    test("Paired-end-stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                input[0] = [
-+                    [ id:'test_paired_end', single_end:false ],
-+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/sra/SRR11140744.tar.gz', checkIfExists: true)
-+                ]
-+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
-+                input[2] = []
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll(
-+            { assert process.success },
-+            { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
- }
-
---- modules/nf-core/sratools/fasterqdump/tests/nextflow.config
-+++ /dev/null
-@@ -1,5 +0,0 @@
--process {
--    withName: SRATOOLS_FASTERQDUMP {
--        ext.args = ''
--    }
--}
-************************************************************
diff --git a/modules/nf-core/sratools/prefetch/sratools-prefetch.diff b/modules/nf-core/sratools/prefetch/sratools-prefetch.diff
deleted file mode 100644
index 25a938e7..00000000
--- a/modules/nf-core/sratools/prefetch/sratools-prefetch.diff
+++ /dev/null
@@ -1,112 +0,0 @@
-Changes in module 'nf-core/sratools/prefetch'
---- modules/nf-core/sratools/prefetch/meta.yml
-+++ modules/nf-core/sratools/prefetch/meta.yml
-@@ -54,3 +54,4 @@
-   - "@Midnighter"
- maintainers:
-   - "@Midnighter"
-+  - "@gallvp"
-
---- modules/nf-core/sratools/prefetch/main.nf
-+++ modules/nf-core/sratools/prefetch/main.nf
-@@ -32,4 +32,16 @@
-     }
- 
-     template 'retry_with_backoff.sh'
-+
-+    stub:
-+    """
-+    mkdir $id
-+    touch $id/${id}.sra
-+
-+    cat <<-END_VERSIONS > versions.yml
-+    "${task.process}":
-+        sratools: \$(prefetch --version 2>&1 | grep -Eo '[0-9.]+')
-+        curl: \$(curl --version | head -n 1 | sed 's/^curl //; s/ .*\$//')
-+    END_VERSIONS
-+    """
- }
-
---- modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
-+++ modules/nf-core/sratools/prefetch/tests/main.nf.test.snap
-@@ -1,4 +1,43 @@
- {
-+    "sratools/prefetch/stub": {
-+        "content": [
-+            {
-+                "0": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            "DRR000774.sra:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "1": [
-+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
-+                ],
-+                "sra": [
-+                    [
-+                        {
-+                            "id": "test",
-+                            "single_end": false
-+                        },
-+                        [
-+                            "DRR000774.sra:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        ]
-+                    ]
-+                ],
-+                "versions": [
-+                    "versions.yml:md5,83d1b23f5ff5b2ad1b96d17d7d7594ee"
-+                ]
-+            }
-+        ],
-+        "meta": {
-+            "nf-test": "0.8.4",
-+            "nextflow": "23.10.1"
-+        },
-+        "timestamp": "2024-05-17T20:07:31.627115"
-+    },
-     "sratools/prefetch with sralite": {
-         "content": [
-             {
-@@ -77,4 +116,4 @@
-         },
-         "timestamp": "2024-02-28T11:48:37.428307"
-     }
--}
-+}
---- modules/nf-core/sratools/prefetch/tests/main.nf.test
-+++ modules/nf-core/sratools/prefetch/tests/main.nf.test
-@@ -46,4 +46,26 @@
-             )
-         }
-     }
-+
-+    test("sratools/prefetch/stub") {
-+
-+        options '-stub'
-+
-+        when {
-+            process {
-+                """
-+                input[0] = Channel.of([ [ id:'test', single_end:false ], 'DRR000774' ])
-+                input[1] = file(params.modules_testdata_base_path + 'generic/config/ncbi_user_settings.mkfg', checkIfExists: true)
-+                input[2] = []
-+                """
-+            }
-+        }
-+
-+        then {
-+            assertAll (
-+            { assert process.success },
-+            { assert snapshot(process.out).match() }
-+            )
-+        }
-+    }
- }
-
-************************************************************
diff --git a/modules/pfr/merqury/hapmers/environment.yml b/modules/pfr/merqury/hapmers/environment.yml
new file mode 100644
index 00000000..5f7977cd
--- /dev/null
+++ b/modules/pfr/merqury/hapmers/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "merqury_hapmers"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - "bioconda::merqury=1.3"
diff --git a/modules/pfr/merqury/hapmers/main.nf b/modules/pfr/merqury/hapmers/main.nf
new file mode 100644
index 00000000..22dd66d5
--- /dev/null
+++ b/modules/pfr/merqury/hapmers/main.nf
@@ -0,0 +1,96 @@
+process MERQURY_HAPMERS {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/merqury:1.3--hdfd78af_1':
+        'biocontainers/merqury:1.3--hdfd78af_1' }"
+
+    input:
+    tuple val(meta), path(child_meryl, stageAs: 'child.meryl')
+    path(maternal_meryl, stageAs: 'mat.meryl')
+    path(paternal_meryl, stageAs: 'pat.meryl')
+
+    output:
+    tuple val(meta), path('*_mat.hapmer.meryl')         , emit: mat_hapmer_meryl
+    tuple val(meta), path('*_pat.hapmer.meryl')         , emit: pat_hapmer_meryl
+    tuple val(meta), path('*_inherited_hapmers.fl.png') , emit: inherited_hapmers_fl_png
+    tuple val(meta), path('*_inherited_hapmers.ln.png') , emit: inherited_hapmers_ln_png
+    tuple val(meta), path('*_inherited_hapmers.st.png') , emit: inherited_hapmers_st_png
+    path "versions.yml"                                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args    = task.ext.args ?: ''
+    def prefix  = task.ext.prefix ?: "${meta.id}"
+    def VERSION = 1.3
+    """
+    # Nextflow changes the container --entrypoint to /bin/bash (container default entrypoint: /usr/local/env-execute)
+    # Check for container variable initialisation script and source it.
+    if [ -f "/usr/local/env-activate.sh" ]; then
+        set +u  # Otherwise, errors out because of various unbound variables
+        . "/usr/local/env-activate.sh"
+        set -u
+    fi
+
+    \$MERQURY/trio/hapmers.sh \\
+        mat.meryl \\
+        pat.meryl\\
+        child.meryl \\
+        $args
+
+    mv mat.hapmer.meryl             ${prefix}_mat.hapmer.meryl
+    mv pat.hapmer.meryl             ${prefix}_pat.hapmer.meryl
+    mv inherited_hapmers.fl.png     ${prefix}_inherited_hapmers.fl.png
+    mv inherited_hapmers.ln.png     ${prefix}_inherited_hapmers.ln.png
+    mv inherited_hapmers.st.png     ${prefix}_inherited_hapmers.st.png
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        merqury: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def args    = task.ext.args ?: ''
+    def prefix  = task.ext.prefix ?: "${meta.id}"
+    def VERSION = 1.3
+    """
+    # Nextflow changes the container --entrypoint to /bin/bash (container default entrypoint: /usr/local/env-execute)
+    # Check for container variable initialisation script and source it.
+    if [ -f "/usr/local/env-activate.sh" ]; then
+        set +u  # Otherwise, errors out because of various unbound variables
+        . "/usr/local/env-activate.sh"
+        set -u
+    fi
+
+    echo \\
+    "\$MERQURY/trio/hapmers.sh \\
+        mat.meryl \\
+        pat.meryl\\
+        child.meryl \\
+        $args"
+
+    mkdir ${prefix}_mat.hapmer.meryl
+    touch ${prefix}_mat.hapmer.meryl/0x000000.merylData
+    touch ${prefix}_mat.hapmer.meryl/0x000000.merylIndex
+    touch ${prefix}_mat.hapmer.meryl/merylIndex
+
+    mkdir ${prefix}_pat.hapmer.meryl
+    touch ${prefix}_pat.hapmer.meryl/0x000000.merylData
+    touch ${prefix}_pat.hapmer.meryl/0x000000.merylIndex
+    touch ${prefix}_pat.hapmer.meryl/merylIndex
+
+    touch ${prefix}_inherited_hapmers.fl.png
+    touch ${prefix}_inherited_hapmers.ln.png
+    touch ${prefix}_inherited_hapmers.st.png
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        merqury: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/merqury/hapmers/meta.yml b/modules/pfr/merqury/hapmers/meta.yml
new file mode 100644
index 00000000..6693f540
--- /dev/null
+++ b/modules/pfr/merqury/hapmers/meta.yml
@@ -0,0 +1,70 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "merqury_hapmers"
+description: A script to generate hap-mer dbs for trios
+keywords:
+  - genomics
+  - quality check
+  - qc
+  - kmer
+tools:
+  - "merqury":
+      description: "Evaluate genome assemblies with k-mers and more."
+      tool_dev_url: "https://github.com/marbl/merqury"
+      doi: "10.1186/s13059-020-02134-9"
+      licence: ["United States Government Work"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - child_meryl:
+      type: directory
+      description: Childs' k-mers (all, from WGS reads)
+      pattern: "*.meryl"
+  - maternal_meryl:
+      type: directory
+      description: Haplotype1 k-mers (all, ex. maternal)
+      pattern: "*.meryl"
+  - paternal_meryl:
+      type: directory
+      description: Haplotype2 k-mers (all, ex. paternal)
+      pattern: "*.meryl"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - mat_hapmer_meryl:
+      type: directory
+      description: Inherited maternal hap-mer dbs
+      pattern: "*_mat.hapmer.meryl"
+  - pat_hapmer_meryl:
+      type: directory
+      description: Inherited paternal hap-mer dbs
+      pattern: "*_pat.hapmer.meryl"
+  - inherited_hapmers_fl_png:
+      type: file
+      description: k-mer distribution of the inherited dbs and cutoffs used to generate hap-mer dbs
+      pattern: "*_inherited_hapmers.fl.png"
+  - inherited_hapmers_ln_png:
+      type: file
+      description: k-mer distribution of the inherited dbs and cutoffs used to generate hap-mer dbs
+      pattern: "*_inherited_hapmers.ln.png"
+  - inherited_hapmers_st_png:
+      type: file
+      description: k-mer distribution of the inherited dbs and cutoffs used to generate hap-mer dbs
+      pattern: "*_inherited_hapmers.st.png"
+
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/modules/pfr/merqury/hapmers/tests/main.nf.test b/modules/pfr/merqury/hapmers/tests/main.nf.test
new file mode 100644
index 00000000..9a254aaf
--- /dev/null
+++ b/modules/pfr/merqury/hapmers/tests/main.nf.test
@@ -0,0 +1,113 @@
+nextflow_process {
+
+    name "Test Process MERQURY_HAPMERS"
+    script "../main.nf"
+    process "MERQURY_HAPMERS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "merqury"
+    tag "merqury/hapmers"
+    tag "meryl/count"
+
+    setup {
+        run('MERYL_COUNT') {
+            script "../../../meryl/count"
+            process {
+                """
+                input[0] = [
+                    [ id:'child' ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test2_1_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = 21
+                """
+            }
+        }
+
+        run('MERYL_COUNT', alias: 'MERYL_COUNT_MATERNAL') {
+            script "../../../meryl/count"
+            process {
+                """
+                input[0] = [
+                    [ id:'maternal' ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = 21
+                """
+            }
+        }
+
+        run('MERYL_COUNT', alias: 'MERYL_COUNT_PATERNAL') {
+            script "../../../meryl/count"
+            process {
+                """
+                input[0] = [
+                    [ id:'paternal' ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = 21
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens") {
+
+        when {
+            process {
+                """
+                input[0] = MERYL_COUNT.out.meryl_db
+                input[1] = MERYL_COUNT_MATERNAL.out.meryl_db.map { meta, db -> db }
+                input[2] = MERYL_COUNT_PATERNAL.out.meryl_db.map { meta, db -> db }
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.mat_hapmer_meryl).md5().match("mat_hapmer_meryl") },
+                { assert snapshot(process.out.pat_hapmer_meryl).md5().match("pat_hapmer_meryl") },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.inherited_hapmers_fl_png[0][1]).name,
+                    file(process.out.inherited_hapmers_ln_png[0][1]).name,
+                    file(process.out.inherited_hapmers_st_png[0][1]).name
+                    ).match()
+                },
+            )
+        }
+    }
+
+    test("homo_sapiens-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = MERYL_COUNT.out.meryl_db
+                input[1] = MERYL_COUNT_MATERNAL.out.meryl_db.map { meta, db -> db }
+                input[2] = MERYL_COUNT_PATERNAL.out.meryl_db.map { meta, db -> db }
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.mat_hapmer_meryl).md5().match("mat_hapmer_meryl_stub") },
+                { assert snapshot(process.out.pat_hapmer_meryl).md5().match("pat_hapmer_meryl_stub") },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.inherited_hapmers_fl_png[0][1]).name,
+                    file(process.out.inherited_hapmers_ln_png[0][1]).name,
+                    file(process.out.inherited_hapmers_st_png[0][1]).name
+                    ).match()
+                },
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/pfr/merqury/hapmers/tests/main.nf.test.snap b/modules/pfr/merqury/hapmers/tests/main.nf.test.snap
new file mode 100644
index 00000000..3a0d4902
--- /dev/null
+++ b/modules/pfr/merqury/hapmers/tests/main.nf.test.snap
@@ -0,0 +1,64 @@
+{
+    "homo_sapiens": {
+        "content": [
+            [
+                "versions.yml:md5,e786e8381bfc64b2fdc423a67f60763e"
+            ],
+            "child_inherited_hapmers.fl.png",
+            "child_inherited_hapmers.ln.png",
+            "child_inherited_hapmers.st.png"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-21T22:26:25.962754"
+    },
+    "mat_hapmer_meryl": {
+        "content": "ccc510b93c249fb07688cf3e4eecd669",
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-21T22:26:25.853929"
+    },
+    "pat_hapmer_meryl": {
+        "content": "003a22d74da4ff265c2224c980d1b9d8",
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-21T22:26:25.88616"
+    },
+    "mat_hapmer_meryl_stub": {
+        "content": "59c648572f6591567baecccf5c3556ed",
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-21T22:26:58.081794"
+    },
+    "pat_hapmer_meryl_stub": {
+        "content": "59c648572f6591567baecccf5c3556ed",
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-21T22:26:58.084766"
+    },
+    "homo_sapiens-stub": {
+        "content": [
+            [
+                "versions.yml:md5,e786e8381bfc64b2fdc423a67f60763e"
+            ],
+            "child_inherited_hapmers.fl.png",
+            "child_inherited_hapmers.ln.png",
+            "child_inherited_hapmers.st.png"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-21T22:26:58.142356"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/merqury/hapmers/tests/tags.yml b/modules/pfr/merqury/hapmers/tests/tags.yml
new file mode 100644
index 00000000..553f09d8
--- /dev/null
+++ b/modules/pfr/merqury/hapmers/tests/tags.yml
@@ -0,0 +1,2 @@
+merqury/hapmers:
+  - "modules/pfr/merqury/hapmers/**"
diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
index 0bc0efd6..a8b381e2 100644
--- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf
@@ -375,8 +375,7 @@ def validateAndNormaliseReadsTuple ( fid, metas, reads, readsType ) {
         error("Please check input assemblysheet -> More than two assemblies (${tags}) are in the same genome group defined by ${identifier}reads_1: ${fid.fid}")
     }
 
-    def groupIDPrefix = readsType == 'reads' ? ( tags.join('-and-') + "-${readsType}-" ) : ''
-    def groupID = groupIDPrefix + fid.fid.replaceAll(/\./, '_')
+    def groupID = readsType == 'reads' ? ( tags.join('-and-') ) : fid.fid.replaceAll(/\./, '_')
 
     if ( metas.first().is_sra ) { // SRA
         return [
diff --git a/tests/merqury.fk/phased2x.mp/params.json b/tests/merqury.fk/phased2x.mp/params.json
deleted file mode 100644
index bc483334..00000000
--- a/tests/merqury.fk/phased2x.mp/params.json
+++ /dev/null
@@ -1,10 +0,0 @@
-{
-    "config_profile_name": "Test profile",
-    "config_profile_description": "Merqury.FK test for a phased diploid assembly contained in separate fasta with parental reads",
-    "input": "tests/merqury.fk/phased2x.mp/assemblysheet.csv",
-    "merqury_skip": false,
-    "merqury_kmer_length": 21,
-    "max_cpus": 8,
-    "max_memory": "32.GB",
-    "max_time": "6.h"
-}
diff --git a/tests/merqury.fk/mixed2x/assemblysheet.csv b/tests/merqury/mixed2x/assemblysheet.csv
similarity index 100%
rename from tests/merqury.fk/mixed2x/assemblysheet.csv
rename to tests/merqury/mixed2x/assemblysheet.csv
diff --git a/tests/merqury.fk/mixed2x/params.json b/tests/merqury/mixed2x/params.json
similarity index 50%
rename from tests/merqury.fk/mixed2x/params.json
rename to tests/merqury/mixed2x/params.json
index 06388889..6ce89d4b 100644
--- a/tests/merqury.fk/mixed2x/params.json
+++ b/tests/merqury/mixed2x/params.json
@@ -1,7 +1,7 @@
 {
     "config_profile_name": "Test profile",
-    "config_profile_description": "Merqury.FK test for a mixed diploid assembly contained in a single fasta",
-    "input": "tests/merqury.fk/mixed2x/assemblysheet.csv",
+    "config_profile_description": "Merqury test for a mixed diploid assembly contained in a single fasta",
+    "input": "tests/merqury/mixed2x/assemblysheet.csv",
     "merqury_skip": false,
     "merqury_kmer_length": 21,
     "max_cpus": 8,
diff --git a/tests/merqury.fk/phased2x.mp/assemblysheet.csv.bk b/tests/merqury/phased2x.mp/assemblysheet.csv
similarity index 100%
rename from tests/merqury.fk/phased2x.mp/assemblysheet.csv.bk
rename to tests/merqury/phased2x.mp/assemblysheet.csv
diff --git a/tests/merqury.fk/phased2x.mp/assemblysheet.csv b/tests/merqury/phased2x.mp/assemblysheet.csv.local
similarity index 84%
rename from tests/merqury.fk/phased2x.mp/assemblysheet.csv
rename to tests/merqury/phased2x.mp/assemblysheet.csv.local
index 5ae40b57..f90aec6f 100644
--- a/tests/merqury.fk/phased2x.mp/assemblysheet.csv
+++ b/tests/merqury/phased2x.mp/assemblysheet.csv.local
@@ -1,4 +1,4 @@
 tag,fasta,reads_1,reads_2,maternal_reads_1,paternal_reads_1
 COL,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fasta,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_2.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_CVI.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_COL.fastq.gz
 CVI,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fasta,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_2.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_CVI.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_COL.fastq.gz
-FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,SRR8238189
+FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR8238189_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR8238189_2.fastq.gz
diff --git a/tests/merqury/phased2x.mp/params.json b/tests/merqury/phased2x.mp/params.json
new file mode 100644
index 00000000..60f157ca
--- /dev/null
+++ b/tests/merqury/phased2x.mp/params.json
@@ -0,0 +1,10 @@
+{
+    "config_profile_name": "Test profile",
+    "config_profile_description": "Merqury test for a phased diploid assembly contained in separate fasta with parental reads",
+    "input": "tests/merqury/phased2x.mp/assemblysheet.csv",
+    "merqury_skip": false,
+    "merqury_kmer_length": 21,
+    "max_cpus": 8,
+    "max_memory": "32.GB",
+    "max_time": "6.h"
+}
diff --git a/tests/merqury.fk/phased2x/assemblysheet.csv b/tests/merqury/phased2x/assemblysheet.csv
similarity index 100%
rename from tests/merqury.fk/phased2x/assemblysheet.csv
rename to tests/merqury/phased2x/assemblysheet.csv
diff --git a/tests/merqury.fk/phased2x/params.json b/tests/merqury/phased2x/params.json
similarity index 50%
rename from tests/merqury.fk/phased2x/params.json
rename to tests/merqury/phased2x/params.json
index 697703b9..0b363fab 100644
--- a/tests/merqury.fk/phased2x/params.json
+++ b/tests/merqury/phased2x/params.json
@@ -1,7 +1,7 @@
 {
     "config_profile_name": "Test profile",
-    "config_profile_description": "Merqury.FK test for a phased diploid assembly contained in separate fasta",
-    "input": "tests/merqury.fk/phased2x/assemblysheet.csv",
+    "config_profile_description": "Merqury test for a phased diploid assembly contained in separate fasta",
+    "input": "tests/merqury/phased2x/assemblysheet.csv",
     "merqury_skip": false,
     "merqury_kmer_length": 21,
     "max_cpus": 8,
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index e5a627b2..e26f3466 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -21,11 +21,14 @@ include { FASTA_KRAKEN2                     } from '../subworkflows/local/fasta_
 include { FQ2HIC                            } from '../subworkflows/local/fq2hic'
 include { CAT_CAT as TAG_ASSEMBLY           } from '../modules/pfr/cat/cat/main'
 include { FASTA_SYNTENY                     } from '../subworkflows/local/fasta_synteny'
-include { FASTK_FASTK                       } from '../modules/nf-core/fastk/fastk/main'
-include { FASTK_FASTK as MATERNAL_FASTK     } from '../modules/nf-core/fastk/fastk/main'
-include { FASTK_FASTK as PATERNAL_FASTK     } from '../modules/nf-core/fastk/fastk/main'
-include { MERQURYFK_HAPMAKER                } from '../modules/local/merquryfk_hapmaker'
-include { MERQURYFK_MERQURYFK               } from '../modules/nf-core/merquryfk/merquryfk/main'
+include { MERYL_COUNT                       } from '../modules/nf-core/meryl/count/main'
+include { MERYL_UNIONSUM                    } from '../modules/nf-core/meryl/unionsum/main'
+include { MERYL_COUNT as MAT_MERYL_COUNT    } from '../modules/nf-core/meryl/count/main'
+include { MERYL_UNIONSUM as MAT_UNIONSUM    } from '../modules/nf-core/meryl/unionsum/main'
+include { MERYL_COUNT as PAT_MERYL_COUNT    } from '../modules/nf-core/meryl/count/main'
+include { MERYL_UNIONSUM as PAT_UNIONSUM    } from '../modules/nf-core/meryl/unionsum/main'
+include { MERQURY_HAPMERS                   } from '../modules/pfr/merqury/hapmers/main'
+include { MERQURY                           } from '../modules/nf-core/merqury/main'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
 include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as FETCHNGS  } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
@@ -541,146 +544,180 @@ workflow ASSEMBLYQC {
     ch_synteny_plots                        = FASTA_SYNTENY.out.png.mix(FASTA_SYNTENY.out.html)
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
-    // MODULE: FASTK_FASTK
+    // MODULE: MERYL_COUNT
     ch_reads_files                          = ch_fetchngs.reads
                                             | mix(ch_reads_branch.rest)
 
-    FASTK_FASTK ( ch_reads_files )
+    MERYL_COUNT(
+        ch_reads_files,
+        params.merqury_kmer_length
+    )
+
+    ch_reads_meryl                          = MERYL_COUNT.out.meryl_db
+    ch_versions                             = ch_versions.mix(MERYL_COUNT.out.versions.first())
+
+    // MODULE: MERYL_UNIONSUM
+    ch_reads_meryl_branch                   = ch_reads_meryl
+                                            | branch { meta, meryl ->
+                                                single: meta.single_end
+                                                paired: ! meta.single_end
+                                            }
+    MERYL_UNIONSUM(
+        ch_reads_meryl_branch.paired,
+        params.merqury_kmer_length
+    )
 
-    ch_reads_fastk_hist                     = FASTK_FASTK.out.hist
-    ch_reads_fastk                          = FASTK_FASTK.out.ktab
-    ch_versions                             = ch_versions.mix(FASTK_FASTK.out.versions.first())
+    ch_reads_union_meryl                    = MERYL_UNIONSUM.out.meryl_db
+                                            | mix(ch_reads_meryl_branch.single)
+    ch_versions                             = ch_versions.mix(MERYL_UNIONSUM.out.versions.first())
 
-    // MODULE: FASTK_FASTK as MATERNAL_FASTK
+    // MODULE: MERYL_COUNT as MAT_MERYL_COUNT
     ch_maternal_reads_files                 = ch_fetchngs.maternal
                                             | mix(ch_maternal_reads_branch.rest)
 
-    MATERNAL_FASTK(
+    MAT_MERYL_COUNT(
         // Guard against failed resume on addition of assemblies with same parents
         ch_maternal_reads_files
-        | map { meta, fq -> [ [ id: meta.id ], fq ] }
+        | map { meta, fq -> [ [ id: meta.id ], fq ] },
+        params.merqury_kmer_length
     )
 
-    // ch_maternal_fastk_hist                  = MATERNAL_FASTK.out.hist
-    //                                         | join(
-    //                                             ch_maternal_reads_files
-    //                                             | map { meta, fq -> [ [ id: meta.id ], meta ] }
-    //                                         )
-    //                                         | map { meta, hist, meta2 -> [ meta2, hist ] }
-
-    ch_maternal_fastk                       = MATERNAL_FASTK.out.ktab
+    ch_maternal_meryl                       = MAT_MERYL_COUNT.out.meryl_db
                                             | join(
                                                 ch_maternal_reads_files
                                                 | map { meta, fq -> [ [ id: meta.id ], meta ] }
                                             )
-                                            | map { meta, ktab, meta2 -> [ meta2, ktab ] }
-    ch_versions                             = ch_versions.mix(MATERNAL_FASTK.out.versions.first())
+                                            | map { meta, meryl, meta2 -> [ meta2, meryl ] }
+    ch_versions                             = ch_versions.mix(MAT_MERYL_COUNT.out.versions.first())
+
+    // MODULE: MAT_UNIONSUM
+    ch_maternal_meryl_branch                = ch_maternal_meryl
+                                            | branch { meta, meryl_db ->
+                                                single: meta.single_end
+                                                paired: ! meta.single_end
+                                            }
+    MAT_UNIONSUM(
+        ch_maternal_meryl_branch.paired,
+        params.merqury_kmer_length
+    )
+
+    ch_maternal_union_meryl                 = MAT_UNIONSUM.out.meryl_db
+                                            | mix(ch_maternal_meryl_branch.single)
+    ch_versions                             = ch_versions.mix(MAT_UNIONSUM.out.versions.first())
 
-    // MODULE: FASTK_FASTK as PATERNAL_FASTK
+    // MODULE: MERYL_COUNT as PAT_MERYL_COUNT
     ch_paternal_reads_files                 = ch_fetchngs.paternal
                                             | mix(ch_paternal_reads_branch.rest)
 
-    PATERNAL_FASTK(
+    PAT_MERYL_COUNT(
         ch_paternal_reads_files
-        | map { meta, fq -> [ [ id: meta.id ], fq ] }
+        | map { meta, fq -> [ [ id: meta.id ], fq ] },
+        params.merqury_kmer_length
     )
 
-    // ch_paternal_fastk_hist                  = PATERNAL_FASTK.out.hist
-    //                                         | join(
-    //                                             ch_paternal_reads_files
-    //                                             | map { meta, fq -> [ [ id: meta.id ], meta ] }
-    //                                         )
-    //                                         | map { meta, hist, meta2 -> [ meta2, hist ] }
-
-    ch_paternal_fastk                       = PATERNAL_FASTK.out.ktab
+    ch_paternal_meryl                       = PAT_MERYL_COUNT.out.meryl_db
                                             | join(
                                                 ch_paternal_reads_files
                                                 | map { meta, fq -> [ [ id: meta.id ], meta ] }
                                             )
-                                            | map { meta, ktab, meta2 -> [ meta2, ktab ] }
-    ch_versions                             = ch_versions.mix(PATERNAL_FASTK.out.versions.first())
+                                            | map { meta, meryl, meta2 -> [ meta2, meryl ] }
+    ch_versions                             = ch_versions.mix(PAT_MERYL_COUNT.out.versions.first())
+
+    // MODULE: PAT_UNIONSUM
+    ch_paternal_meryl_branch                = ch_paternal_meryl
+                                            | branch { meta, meryl ->
+                                                single: meta.single_end
+                                                paired: ! meta.single_end
+                                            }
+    PAT_UNIONSUM(
+        ch_paternal_meryl_branch.paired,
+        params.merqury_kmer_length
+    )
 
-    // MODULE: MERQURYFK_HAPMAKER
-    ch_all_assemblies_with_parents          = ch_maternal_fastk
-                                            | mix(ch_paternal_fastk)
-                                            | flatMap { meta, ktab -> meta.assemblies }
+    ch_paternal_union_meryl                 = PAT_UNIONSUM.out.meryl_db
+                                            | mix(ch_paternal_meryl_branch.single)
+    ch_versions                             = ch_versions.mix(PAT_UNIONSUM.out.versions.first())
+
+    // MODULE: MERQURY_HAPMERS
+    ch_all_assemblies_with_parents          = ch_maternal_union_meryl
+                                            | mix(ch_paternal_union_meryl)
+                                            | flatMap { meta, meryl -> meta.assemblies }
                                             | unique
                                             | collect
                                             | map { [ it ] }
                                             | ifEmpty( [ [] ] )
 
-    ch_reads_fastk_without_parents          = ch_reads_fastk
+    ch_meryl_without_parents                = ch_reads_union_meryl
                                             | combine(
                                                 ch_all_assemblies_with_parents
                                             )
-                                            | filter { meta, ktab, p_asms -> ! meta.assemblies.any { it in p_asms } }
-                                            | map { meta, ktab, p_asms -> [ meta, ktab, [], [] ] }
+                                            | filter { meta, meryl, p_asms -> ! meta.assemblies.any { it in p_asms } }
+                                            | map { meta, meryl, p_asms -> [ meta, meryl, [], [] ] }
 
-    ch_group_fastk                          = ch_reads_fastk
-                                            | combine ( ch_maternal_fastk )
-                                            | filter { meta, ktab, meta2, mktab ->
+    ch_group_meryl                          = ch_reads_union_meryl
+                                            | combine ( ch_maternal_union_meryl )
+                                            | filter { meta, meryl, meta2, mat_meryl ->
                                                 meta.assemblies.every { it in meta2.assemblies }
                                             }
-                                            | map { meta, ktab, meta2, mktab ->
-                                                [ meta, ktab, mktab ]
+                                            | map { meta, meryl, meta2, mat_meryl ->
+                                                [ meta, meryl, mat_meryl ]
                                             }
-                                            | combine ( ch_paternal_fastk )
-                                            | filter { meta, ktab, mktab, meta2, pktab ->
+                                            | combine ( ch_paternal_union_meryl )
+                                            | filter { meta, meryl, mat_meryl, meta2, pat_meryl ->
                                                 meta.assemblies.every { it in meta2.assemblies }
                                             }
-                                            | map { meta, ktab, mktab, meta2, pktab ->
-                                                [ meta, ktab, mktab, pktab ]
+                                            | map { meta, meryl, mat_meryl, meta2, pat_meryl ->
+                                                [ meta, meryl, mat_meryl, pat_meryl ]
                                             }
 
-    MERQURYFK_HAPMAKER(
-        ch_group_fastk.map { meta, ktab, mktab, pktab -> [ meta, ktab ] },
-        ch_group_fastk.map { meta, ktab, mktab, pktab -> mktab },
-        ch_group_fastk.map { meta, ktab, mktab, pktab -> pktab }
+    MERQURY_HAPMERS(
+        ch_group_meryl.map { meta, meryl, mat_meryl, pat_meryl -> [ meta, meryl ] },
+        ch_group_meryl.map { meta, meryl, mat_meryl, pat_meryl -> mat_meryl },
+        ch_group_meryl.map { meta, meryl, mat_meryl, pat_meryl -> pat_meryl }
     )
 
-    ch_parental_hapmers                     = MERQURYFK_HAPMAKER.out.mat_hapmers
-                                            | join(MERQURYFK_HAPMAKER.out.pat_hapmers)
-    ch_versions                             = ch_versions.mix(MERQURYFK_HAPMAKER.out.versions.first())
+    ch_parental_hapmers                     = MERQURY_HAPMERS.out.mat_hapmer_meryl
+                                            | join(MERQURY_HAPMERS.out.pat_hapmer_meryl)
+    ch_versions                             = ch_versions.mix(MERQURY_HAPMERS.out.versions.first())
 
-    // Prepare FASTK tables
-    ch_fastk_ktabs                          = ch_group_fastk
+    // Prepare group meryl dbs
+    ch_meryl_all                            = ch_group_meryl
                                             | join(ch_parental_hapmers)
-                                            | map { meta, ktab, mktab, pktab, hap_mktab, hap_pktab ->
-                                                [ meta, ktab, hap_mktab, hap_pktab ]
+                                            | map { meta, meryl, mat_meryl, pat_meryl, hap_mat_meryl, hap_pat_meryl ->
+                                                [ meta, meryl, hap_mat_meryl, hap_pat_meryl ]
                                             }
-                                            | mix(ch_reads_fastk_without_parents)
-                                            | map { meta, ktab, hap_mktab, hap_pktab ->
+                                            | mix(ch_meryl_without_parents)
+                                            | map { meta, meryl, mat_meryl, pat_meryl ->
                                                 [
                                                     meta,
-                                                    ktab + hap_mktab + hap_pktab
+                                                    mat_meryl
+                                                    ? [ meryl, mat_meryl, pat_meryl ]
+                                                    : meryl
                                                 ]
                                             }
 
-    // MODULE: MERQURYFK_MERQURYFK
-    ch_merqury_inputs                       = ch_reads_fastk_hist
-                                            | join(
-                                                ch_fastk_ktabs
-                                            )
+    // MODULE: MERQURY
+    ch_merqury_inputs                       = ch_meryl_all
                                             | join(
                                                 ch_reads_assemblies
                                                 | map { meta, fq, fastas -> [ meta, fastas ] }
                                             )
-                                            | map { meta, hist, ktab, fastas ->
-                                                fastas.size() > 1
-                                                ? [ meta, hist, ktab, fastas[0], fastas[1] ]
-                                                : [ meta, hist, ktab, fastas[0], [] ]
-                                            }
 
-    MERQURYFK_MERQURYFK ( ch_merqury_inputs )
+    MERQURY ( ch_merqury_inputs )
+
+    ch_merqury_qv                           = MERQURY.out.assembly_qv
+    ch_merqury_stats                        = MERQURY.out.stats
+    ch_merqury_spectra_cn_fl_png            = MERQURY.out.spectra_cn_fl_png
+    ch_merqury_spectra_asm_fl_png           = MERQURY.out.spectra_asm_fl_png
+    ch_hapmers_blob_png                     = MERQURY.out.hapmers_blob_png
 
-    ch_merqury_qv                           = MERQURYFK_MERQURYFK.out.qv
-    ch_merqury_stats                        = MERQURYFK_MERQURYFK.out.stats
-    ch_merqury_spectra_cn_fl_png            = MERQURYFK_MERQURYFK.out.spectra_cn_fl_png
     ch_merqury_outputs                      = ch_merqury_qv
                                             | mix(ch_merqury_stats)
                                             | mix(ch_merqury_spectra_cn_fl_png)
+                                            | mix(ch_merqury_spectra_asm_fl_png)
+                                            | mix(ch_hapmers_blob_png)
                                             | flatMap { meta, data -> data }
-    ch_versions                             = ch_versions.mix(MERQURYFK_MERQURYFK.out.versions.first())
+    ch_versions                             = ch_versions.mix(MERQURY.out.versions.first())
 
     // Collate and save software versions
     ch_versions                             = ch_versions
@@ -698,25 +735,25 @@ workflow ASSEMBLYQC {
                                             )
 
     // MODULE: CREATEREPORT
-    // CREATEREPORT(
-    //     ch_invalid_assembly_log             .collect().ifEmpty([]),
-    //     ch_invalid_gff3_log                 .collect().ifEmpty([]),
-    //     ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
-    //     ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
-    //     ch_assemblathon_stats               .collect().ifEmpty([]),
-    //     ch_gt_stats                         .collect().ifEmpty([]),
-    //     ch_busco_outputs                    .collect().ifEmpty([]),
-    //     ch_busco_gff_outputs                .collect().ifEmpty([]),
-    //     ch_tidk_outputs                     .collect().ifEmpty([]),
-    //     ch_lai_outputs                      .collect().ifEmpty([]),
-    //     ch_kraken2_plot                     .collect().ifEmpty([]),
-    //     ch_hic_html                         .collect().ifEmpty([]),
-    //     ch_synteny_plots                    .collect().ifEmpty([]),
-    //     ch_merqury_outputs                  .collect().ifEmpty([]),
-    //     ch_versions_yml,
-    //     ch_params_as_json,
-    //     ch_summary_params_as_json
-    // )
+    CREATEREPORT(
+        ch_invalid_assembly_log             .collect().ifEmpty([]),
+        ch_invalid_gff3_log                 .collect().ifEmpty([]),
+        ch_fcs_adaptor_report               .map { meta, file -> file }.collect().ifEmpty([]),
+        ch_fcs_gx_report                    .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]),
+        ch_assemblathon_stats               .collect().ifEmpty([]),
+        ch_gt_stats                         .collect().ifEmpty([]),
+        ch_busco_outputs                    .collect().ifEmpty([]),
+        ch_busco_gff_outputs                .collect().ifEmpty([]),
+        ch_tidk_outputs                     .collect().ifEmpty([]),
+        ch_lai_outputs                      .collect().ifEmpty([]),
+        ch_kraken2_plot                     .collect().ifEmpty([]),
+        ch_hic_html                         .collect().ifEmpty([]),
+        ch_synteny_plots                    .collect().ifEmpty([]),
+        ch_merqury_outputs                  .collect().ifEmpty([]),
+        ch_versions_yml,
+        ch_params_as_json,
+        ch_summary_params_as_json
+    )
 }
 
 /*

From dd743ddb9392982b87a6c54ce8bb41de609e5908 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 22 May 2024 16:08:04 +1200
Subject: [PATCH 48/76] Locally patched MERQURY, MERYL/COUNT and MERYL/UNIONSUM

---
 CHANGELOG.md                                  |  16 +-
 modules.json                                  |  15 +-
 modules/nf-core/merqury/merqury.diff          |  13 +
 modules/nf-core/meryl/count/main.nf           |   9 +-
 modules/nf-core/meryl/count/meryl-count.diff  |  79 +++++
 modules/nf-core/meryl/count/meta.yml          |   3 +-
 .../meryl/count/tests/main.nf.test.snap       |   6 +-
 modules/nf-core/meryl/unionsum/main.nf        |   5 +-
 .../meryl/unionsum/meryl-unionsum.diff        |  78 +++++
 modules/nf-core/meryl/unionsum/meta.yml       |   3 +-
 .../meryl/unionsum/tests/main.nf.test.snap    |   6 +-
 modules/pfr/bwa/index/meta.yml                |   1 +
 modules/pfr/bwa/index/tests/main.nf.test      |   2 +-
 modules/pfr/bwa/index/tests/main.nf.test.snap |  10 +-
 modules/pfr/bwa/mem/environment.yml           |   6 +-
 modules/pfr/bwa/mem/main.nf                   |   4 +-
 modules/pfr/bwa/mem/tests/main.nf.test        |  45 ++-
 modules/pfr/bwa/mem/tests/main.nf.test.snap   | 303 +++++-------------
 .../tests/main.nf.test.snap                   |  24 +-
 19 files changed, 345 insertions(+), 283 deletions(-)
 create mode 100644 modules/nf-core/merqury/merqury.diff
 create mode 100644 modules/nf-core/meryl/count/meryl-count.diff
 create mode 100644 modules/nf-core/meryl/unionsum/meryl-unionsum.diff

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d9feb3ed..c340fc9e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -14,14 +14,14 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 5. Added a custom `BUNDLELINKS` module which respects direction when bundling `DNADIFF` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
 6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
 7. Updated modules and sub-workflows: `BWA/INDEX`, `BWA/MEM`, `CAT/CAT`, `CUSTOM/CHECKGFF3FASTACORRESPONDENCE`, `CUSTOM/RESTOREGFFIDS`, `CUSTOM/SHORTENFASTAIDS`, `GT/GFF3`, `GT/GFF3VALIDATOR`, `GT/STAT`, `LTRFINDER`, `LTRHARVEST`, `LTRRETRIEVER/LAI`, `LTRRETRIEVER/LTRRETRIEVER`, `SAMBLASTER`, `FASTA_LTRRETRIEVER_LAI`, `FASTQ_BWA_MEM_SAMBLASTER`, `GFF3_VALIDATE`, `CUSTOM/SRATOOLSNCBISETTINGS`, `FASTP`, `FASTQC`, `UNTAR`, `SEQKIT/SEQ`, `SEQKIT/SORT`, `FASTA_EXPLORE_SEARCH_PLOT_TIDK`
-8. Locally bumped `bwa/index` version to `biocontainers/bwa:0.7.18--he4a0461_0`
-9. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
-10. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
-11. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
-12. Added `MERQURY` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
-13. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
-14. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
-15. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
+8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
+9. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
+10. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
+11. Added `MERQURY` module [#85](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/85)
+12. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
+13. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
+14. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
+15. Locally patched `MERQURY`, `MERYL/COUNT` and `MERYL/UNIONSUM`
 
 ### `Fixed`
 
diff --git a/modules.json b/modules.json
index 188bf227..b60aa0ff 100644
--- a/modules.json
+++ b/modules.json
@@ -17,12 +17,12 @@
                     },
                     "bwa/index": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "d158062346649b5d55bccef77f4bbd0468760aff",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "bwa/mem": {
                         "branch": "main",
-                        "git_sha": "26eca75b7373d582569353ed70817072f908b2da",
+                        "git_sha": "d158062346649b5d55bccef77f4bbd0468760aff",
                         "installed_by": ["fastq_bwa_mem_samblaster"]
                     },
                     "cat/cat": {
@@ -111,7 +111,7 @@
                     },
                     "fastq_bwa_mem_samblaster": {
                         "branch": "main",
-                        "git_sha": "258dbf212876e040e0049809ff0f9fc3f032b8d9",
+                        "git_sha": "d158062346649b5d55bccef77f4bbd0468760aff",
                         "installed_by": ["subworkflows"]
                     },
                     "gff3_gt_gff3_gff3validator_stat": {
@@ -158,17 +158,20 @@
                     "merqury": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/merqury/merqury.diff"
                     },
                     "meryl/count": {
                         "branch": "master",
                         "git_sha": "c95215baf20e6d8cf2ae0e8411d03c5d9ef21d4a",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/meryl/count/meryl-count.diff"
                     },
                     "meryl/unionsum": {
                         "branch": "master",
                         "git_sha": "b7f584e61c35c138a5717f837f4da9b838e290a7",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/meryl/unionsum/meryl-unionsum.diff"
                     },
                     "seqkit/seq": {
                         "branch": "master",
diff --git a/modules/nf-core/merqury/merqury.diff b/modules/nf-core/merqury/merqury.diff
new file mode 100644
index 00000000..d5ba84f6
--- /dev/null
+++ b/modules/nf-core/merqury/merqury.diff
@@ -0,0 +1,13 @@
+Changes in module 'nf-core/merqury'
+--- modules/nf-core/merqury/main.nf
++++ modules/nf-core/merqury/main.nf
+@@ -26,6 +26,7 @@
+     tuple val(meta), path("${prefix}.qv")        , emit: assembly_qv
+     tuple val(meta), path("${prefix}.*.qv")      , emit: scaffold_qv
+     tuple val(meta), path("*.hist.ploidy")       , emit: read_ploidy
++    tuple val(meta), path("*.hapmers.blob.png")  , emit: hapmers_blob_png           , optional: true
+     path "versions.yml"                          , emit: versions
+ 
+     when:
+
+************************************************************
diff --git a/modules/nf-core/meryl/count/main.nf b/modules/nf-core/meryl/count/main.nf
index 2f0518f3..0f1ff0af 100644
--- a/modules/nf-core/meryl/count/main.nf
+++ b/modules/nf-core/meryl/count/main.nf
@@ -12,8 +12,8 @@ process MERYL_COUNT {
     val kvalue
 
     output:
-    tuple val(meta), path("*.meryldb"), emit: meryl_db
-    path "versions.yml"               , emit: versions
+    tuple val(meta), path("*.meryl")    , emit: meryl_db
+    path "versions.yml"                 , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,9 +26,10 @@ process MERYL_COUNT {
         meryl count \\
             k=$kvalue \\
             threads=$task.cpus \\
+            memory=${task.memory.toGiga()} \\
             $args \\
             $reads \\
-            output read.\${READ%.f*}.meryldb
+            output read.\${READ%.f*}.meryl
     done
 
     cat <<-END_VERSIONS > versions.yml
@@ -42,7 +43,7 @@ process MERYL_COUNT {
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     for READ in $reads; do
-        touch read.\${READ%.f*}.meryldb
+        touch read.\${READ%.f*}.meryl
     done
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/meryl/count/meryl-count.diff b/modules/nf-core/meryl/count/meryl-count.diff
new file mode 100644
index 00000000..4bdabc19
--- /dev/null
+++ b/modules/nf-core/meryl/count/meryl-count.diff
@@ -0,0 +1,79 @@
+Changes in module 'nf-core/meryl/count'
+--- modules/nf-core/meryl/count/meta.yml
++++ modules/nf-core/meryl/count/meta.yml
+@@ -38,8 +38,9 @@
+   - meryl_db:
+       type: directory
+       description: A Meryl k-mer database
+-      pattern: "*.meryldb"
++      pattern: "*.meryl"
+ authors:
+   - "@mahesh-panchal"
+ maintainers:
+   - "@mahesh-panchal"
++  - "@gallvp"
+
+--- modules/nf-core/meryl/count/main.nf
++++ modules/nf-core/meryl/count/main.nf
+@@ -12,8 +12,8 @@
+     val kvalue
+ 
+     output:
+-    tuple val(meta), path("*.meryldb"), emit: meryl_db
+-    path "versions.yml"               , emit: versions
++    tuple val(meta), path("*.meryl")    , emit: meryl_db
++    path "versions.yml"                 , emit: versions
+ 
+     when:
+     task.ext.when == null || task.ext.when
+@@ -26,9 +26,10 @@
+         meryl count \\
+             k=$kvalue \\
+             threads=$task.cpus \\
++            memory=${task.memory.toGiga()} \\
+             $args \\
+             $reads \\
+-            output read.\${READ%.f*}.meryldb
++            output read.\${READ%.f*}.meryl
+     done
+ 
+     cat <<-END_VERSIONS > versions.yml
+@@ -42,7 +43,7 @@
+     def prefix = task.ext.prefix ?: "${meta.id}"
+     """
+     for READ in $reads; do
+-        touch read.\${READ%.f*}.meryldb
++        touch read.\${READ%.f*}.meryl
+     done
+ 
+     cat <<-END_VERSIONS > versions.yml
+
+--- modules/nf-core/meryl/count/tests/main.nf.test.snap
++++ modules/nf-core/meryl/count/tests/main.nf.test.snap
+@@ -303,7 +303,7 @@
+                             "id": "test",
+                             "single_end": true
+                         },
+-                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        "read.test1_1.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
+                     ]
+                 ],
+                 "1": [
+@@ -315,7 +315,7 @@
+                             "id": "test",
+                             "single_end": true
+                         },
+-                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        "read.test1_1.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
+                     ]
+                 ],
+                 "versions": [
+@@ -327,6 +327,6 @@
+             "nf-test": "0.8.4",
+             "nextflow": "23.10.1"
+         },
+-        "timestamp": "2024-03-26T19:30:13.745468405"
++        "timestamp": "2024-05-22T12:27:16.300916"
+     }
+ }
+************************************************************
diff --git a/modules/nf-core/meryl/count/meta.yml b/modules/nf-core/meryl/count/meta.yml
index c9347c0c..809a32fe 100644
--- a/modules/nf-core/meryl/count/meta.yml
+++ b/modules/nf-core/meryl/count/meta.yml
@@ -38,8 +38,9 @@ output:
   - meryl_db:
       type: directory
       description: A Meryl k-mer database
-      pattern: "*.meryldb"
+      pattern: "*.meryl"
 authors:
   - "@mahesh-panchal"
 maintainers:
   - "@mahesh-panchal"
+  - "@gallvp"
diff --git a/modules/nf-core/meryl/count/tests/main.nf.test.snap b/modules/nf-core/meryl/count/tests/main.nf.test.snap
index da7c9f31..e0a20902 100644
--- a/modules/nf-core/meryl/count/tests/main.nf.test.snap
+++ b/modules/nf-core/meryl/count/tests/main.nf.test.snap
@@ -303,7 +303,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "read.test1_1.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "1": [
@@ -315,7 +315,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "read.test1_1.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "versions": [
@@ -327,6 +327,6 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-26T19:30:13.745468405"
+        "timestamp": "2024-05-22T12:27:16.300916"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/meryl/unionsum/main.nf b/modules/nf-core/meryl/unionsum/main.nf
index 9800f605..bc2853b0 100644
--- a/modules/nf-core/meryl/unionsum/main.nf
+++ b/modules/nf-core/meryl/unionsum/main.nf
@@ -12,8 +12,8 @@ process MERYL_UNIONSUM {
     val kvalue
 
     output:
-    tuple val(meta), path("*.unionsum.meryl")   , emit: meryl_db
-    path "versions.yml"                         , emit: versions
+    tuple val(meta), path("*.unionsum.meryl"), emit: meryl_db
+    path "versions.yml"                        , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,6 +25,7 @@ process MERYL_UNIONSUM {
     meryl union-sum \\
         k=$kvalue \\
         threads=$task.cpus \\
+        memory=${task.memory.toGiga()} \\
         $args \\
         output ${prefix}.unionsum.meryl \\
         $meryl_dbs
diff --git a/modules/nf-core/meryl/unionsum/meryl-unionsum.diff b/modules/nf-core/meryl/unionsum/meryl-unionsum.diff
new file mode 100644
index 00000000..3a8058c1
--- /dev/null
+++ b/modules/nf-core/meryl/unionsum/meryl-unionsum.diff
@@ -0,0 +1,78 @@
+Changes in module 'nf-core/meryl/unionsum'
+--- modules/nf-core/meryl/unionsum/meta.yml
++++ modules/nf-core/meryl/unionsum/meta.yml
+@@ -36,8 +36,9 @@
+   - meryl_db:
+       type: directory
+       description: A Meryl k-mer database that is the union sum of the input databases
+-      pattern: "*.unionsum.meryldb"
++      pattern: "*.unionsum.meryl"
+ authors:
+   - "@mahesh-panchal"
+ maintainers:
+   - "@mahesh-panchal"
++  - "@gallvp"
+
+--- modules/nf-core/meryl/unionsum/main.nf
++++ modules/nf-core/meryl/unionsum/main.nf
+@@ -12,7 +12,7 @@
+     val kvalue
+ 
+     output:
+-    tuple val(meta), path("*.unionsum.meryldb"), emit: meryl_db
++    tuple val(meta), path("*.unionsum.meryl"), emit: meryl_db
+     path "versions.yml"                        , emit: versions
+ 
+     when:
+@@ -25,8 +25,9 @@
+     meryl union-sum \\
+         k=$kvalue \\
+         threads=$task.cpus \\
++        memory=${task.memory.toGiga()} \\
+         $args \\
+-        output ${prefix}.unionsum.meryldb \\
++        output ${prefix}.unionsum.meryl \\
+         $meryl_dbs
+ 
+     cat <<-END_VERSIONS > versions.yml
+@@ -39,7 +40,7 @@
+     def args = task.ext.args ?: ''
+     def prefix = task.ext.prefix ?: "${meta.id}"
+     """
+-    touch ${prefix}.unionsum.meryldb
++    touch ${prefix}.unionsum.meryl
+ 
+     cat <<-END_VERSIONS > versions.yml
+     "${task.process}":
+
+--- modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
++++ modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
+@@ -8,7 +8,7 @@
+                             "id": "test",
+                             "single_end": true
+                         },
+-                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        "test.unionsum.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
+                     ]
+                 ],
+                 "1": [
+@@ -20,7 +20,7 @@
+                             "id": "test",
+                             "single_end": true
+                         },
+-                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
++                        "test.unionsum.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
+                     ]
+                 ],
+                 "versions": [
+@@ -32,7 +32,7 @@
+             "nf-test": "0.8.4",
+             "nextflow": "23.10.1"
+         },
+-        "timestamp": "2024-03-27T10:19:52.291287389"
++        "timestamp": "2024-05-22T12:40:21.306142"
+     },
+     "sarscov2 - fastq - single_end": {
+         "content": [
+
+************************************************************
diff --git a/modules/nf-core/meryl/unionsum/meta.yml b/modules/nf-core/meryl/unionsum/meta.yml
index 3bb01250..77d0784c 100644
--- a/modules/nf-core/meryl/unionsum/meta.yml
+++ b/modules/nf-core/meryl/unionsum/meta.yml
@@ -36,8 +36,9 @@ output:
   - meryl_db:
       type: directory
       description: A Meryl k-mer database that is the union sum of the input databases
-      pattern: "*.unionsum.meryldb"
+      pattern: "*.unionsum.meryl"
 authors:
   - "@mahesh-panchal"
 maintainers:
   - "@mahesh-panchal"
+  - "@gallvp"
diff --git a/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap b/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
index 522a52c9..a6ed4c73 100644
--- a/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
+++ b/modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
@@ -8,7 +8,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.unionsum.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "1": [
@@ -20,7 +20,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.unionsum.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "versions": [
@@ -32,7 +32,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-27T10:19:52.291287389"
+        "timestamp": "2024-05-22T12:40:21.306142"
     },
     "sarscov2 - fastq - single_end": {
         "content": [
diff --git a/modules/pfr/bwa/index/meta.yml b/modules/pfr/bwa/index/meta.yml
index 730628d0..4c7d30f3 100644
--- a/modules/pfr/bwa/index/meta.yml
+++ b/modules/pfr/bwa/index/meta.yml
@@ -43,3 +43,4 @@ authors:
 maintainers:
   - "@drpatelh"
   - "@maxulysse"
+  - "@gallvp"
diff --git a/modules/pfr/bwa/index/tests/main.nf.test b/modules/pfr/bwa/index/tests/main.nf.test
index b0d2c726..af33e73c 100644
--- a/modules/pfr/bwa/index/tests/main.nf.test
+++ b/modules/pfr/bwa/index/tests/main.nf.test
@@ -30,4 +30,4 @@ nextflow_process {
 
     }
 
-}
\ No newline at end of file
+}
diff --git a/modules/pfr/bwa/index/tests/main.nf.test.snap b/modules/pfr/bwa/index/tests/main.nf.test.snap
index e51ad5bf..7c8f0465 100644
--- a/modules/pfr/bwa/index/tests/main.nf.test.snap
+++ b/modules/pfr/bwa/index/tests/main.nf.test.snap
@@ -17,7 +17,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f"
+                    "versions.yml:md5,a64462ac7dfb21f4ade9b02e7f65c5bb"
                 ],
                 "index": [
                     [
@@ -34,10 +34,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f"
+                    "versions.yml:md5,a64462ac7dfb21f4ade9b02e7f65c5bb"
                 ]
             }
         ],
-        "timestamp": "2023-10-17T17:20:20.180927714"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-16T11:40:09.925307"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/bwa/mem/environment.yml b/modules/pfr/bwa/mem/environment.yml
index 3f136d0a..3aa9f0cc 100644
--- a/modules/pfr/bwa/mem/environment.yml
+++ b/modules/pfr/bwa/mem/environment.yml
@@ -4,7 +4,7 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bwa=0.7.17
+  - bwa=0.7.18
   # renovate: datasource=conda depName=bioconda/samtools
-  - samtools=1.19.2
-  - htslib=1.19.1
+  - samtools=1.20
+  - htslib=1.20.0
diff --git a/modules/pfr/bwa/mem/main.nf b/modules/pfr/bwa/mem/main.nf
index d8bd2815..9c815f0c 100644
--- a/modules/pfr/bwa/mem/main.nf
+++ b/modules/pfr/bwa/mem/main.nf
@@ -4,8 +4,8 @@ process BWA_MEM {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' :
-        'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' }"
+        'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:1bd8542a8a0b42e0981337910954371d0230828e-0' :
+        'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:1bd8542a8a0b42e0981337910954371d0230828e-0' }"
 
     input:
     tuple val(meta) , path(reads)
diff --git a/modules/pfr/bwa/mem/tests/main.nf.test b/modules/pfr/bwa/mem/tests/main.nf.test
index 1fa9b56d..463b76f8 100644
--- a/modules/pfr/bwa/mem/tests/main.nf.test
+++ b/modules/pfr/bwa/mem/tests/main.nf.test
@@ -44,7 +44,14 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.cram,
+                    process.out.csi,
+                    process.out.crai,
+                    process.out.versions,
+                    file(process.out.bam[0][1]).name
+                    ).match()
+                }
             )
         }
 
@@ -85,7 +92,14 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.cram,
+                    process.out.csi,
+                    process.out.crai,
+                    process.out.versions,
+                    file(process.out.bam[0][1]).name
+                    ).match()
+                }
             )
         }
 
@@ -127,7 +141,14 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.cram,
+                    process.out.csi,
+                    process.out.crai,
+                    process.out.versions,
+                    file(process.out.bam[0][1]).name
+                    ).match()
+                }
             )
         }
 
@@ -169,7 +190,14 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.cram,
+                    process.out.csi,
+                    process.out.crai,
+                    process.out.versions,
+                    file(process.out.bam[0][1]).name
+                    ).match()
+                }
             )
         }
 
@@ -211,7 +239,14 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.cram,
+                    process.out.csi,
+                    process.out.crai,
+                    process.out.versions,
+                    file(process.out.bam[0][1]).name
+                    ).match()
+                }
             )
         }
 
diff --git a/modules/pfr/bwa/mem/tests/main.nf.test.snap b/modules/pfr/bwa/mem/tests/main.nf.test.snap
index 0d1bdb41..038ee7b7 100644
--- a/modules/pfr/bwa/mem/tests/main.nf.test.snap
+++ b/modules/pfr/bwa/mem/tests/main.nf.test.snap
@@ -1,295 +1,140 @@
 {
     "Single-End": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.bam:md5,a74710a0345b4717bb4431bf9c257120"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.bam:md5,a74710a0345b4717bb4431bf9c257120"
-                    ]
-                ],
-                "crai": [
-                    
-                ],
-                "cram": [
-                    
-                ],
-                "csi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ]
-            }
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
+            ],
+            "test.bam"
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-14T14:14:56.820798254"
+        "timestamp": "2024-05-20T08:44:32.953673185"
     },
     "Single-End Sort": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.bam:md5,cb1e038bc4d990683fa485d632550b54"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": true
-                        },
-                        "test.bam:md5,cb1e038bc4d990683fa485d632550b54"
-                    ]
-                ],
-                "crai": [
-                    
-                ],
-                "cram": [
-                    
-                ],
-                "csi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ]
-            }
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
+            ],
+            "test.bam"
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-14T14:15:20.271428534"
+        "timestamp": "2024-05-20T08:44:45.27066093"
     },
     "Paired-End": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,aea123a3828a99da1906126355f15a12"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,aea123a3828a99da1906126355f15a12"
-                    ]
-                ],
-                "crai": [
-                    
-                ],
-                "cram": [
-                    
-                ],
-                "csi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ]
-            }
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
+            ],
+            "test.bam"
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-14T14:15:38.264256823"
+        "timestamp": "2024-05-20T08:44:57.706852274"
     },
     "Paired-End Sort": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,4682087bcdc3617384b375093fecd8dd"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,4682087bcdc3617384b375093fecd8dd"
-                    ]
-                ],
-                "crai": [
-                    
-                ],
-                "cram": [
-                    
-                ],
-                "csi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ]
-            }
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
+            ],
+            "test.bam"
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-14T14:16:00.528642686"
+        "timestamp": "2024-05-20T08:45:10.376505036"
     },
     "Single-end - stub": {
         "content": [
             "test.bam",
             "test.csi",
             [
-                "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-15T09:50:33.238543011"
+        "timestamp": "2024-05-20T08:46:07.182072398"
     },
     "Paired-End - no fasta": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,aea123a3828a99da1906126355f15a12"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    
-                ],
-                "4": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,aea123a3828a99da1906126355f15a12"
-                    ]
-                ],
-                "crai": [
-                    
-                ],
-                "cram": [
-                    
-                ],
-                "csi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
-                ]
-            }
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
+            ],
+            "test.bam"
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-14T14:40:16.345342005"
+        "timestamp": "2024-05-20T08:45:53.813076501"
     },
     "Paired-end - stub": {
         "content": [
             "test.bam",
             "test.csi",
             [
-                "versions.yml:md5,c32f719a68bb2966c8511d808154d42d"
+                "versions.yml:md5,478b816fbd37871f5e8c617833d51d80"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-15T09:50:42.268673302"
+        "timestamp": "2024-05-20T08:46:18.412916364"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap
index 83f6bc6d..a2b24322 100644
--- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap
+++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap
@@ -8,13 +8,13 @@
                             "id": "test",
                             "ref_id": "genome"
                         },
-                        "test.on.genome.samblaster.bam:md5,8c47b2669f5250ad2d24365b42566d78"
+                        "test.on.genome.samblaster.bam:md5,38ade4c8ae74d49a197286e09a6cb3ad"
                     ]
                 ],
                 "1": [
                     "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
-                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
-                    "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
+                    "versions.yml:md5,80949a94ee1be8238b1c79d89ed9fa83",
+                    "versions.yml:md5,ddd810edeee1008ec55b628e3a9d6204"
                 ],
                 "bam": [
                     [
@@ -22,13 +22,13 @@
                             "id": "test",
                             "ref_id": "genome"
                         },
-                        "test.on.genome.samblaster.bam:md5,8c47b2669f5250ad2d24365b42566d78"
+                        "test.on.genome.samblaster.bam:md5,38ade4c8ae74d49a197286e09a6cb3ad"
                     ]
                 ],
                 "versions": [
                     "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
-                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
-                    "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
+                    "versions.yml:md5,80949a94ee1be8238b1c79d89ed9fa83",
+                    "versions.yml:md5,ddd810edeee1008ec55b628e3a9d6204"
                 ]
             }
         ],
@@ -36,7 +36,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-05-03T15:58:33.221762"
+        "timestamp": "2024-05-22T15:55:44.323082"
     },
     "sarscov2-fq-gz-stub": {
         "content": [
@@ -52,8 +52,8 @@
                 ],
                 "1": [
                     "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
-                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
-                    "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
+                    "versions.yml:md5,80949a94ee1be8238b1c79d89ed9fa83",
+                    "versions.yml:md5,ddd810edeee1008ec55b628e3a9d6204"
                 ],
                 "bam": [
                     [
@@ -66,8 +66,8 @@
                 ],
                 "versions": [
                     "versions.yml:md5,3b59736950270bca59c29afeb5289b4f",
-                    "versions.yml:md5,46f2d5384c2b4c6111293bc8642cc43a",
-                    "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b"
+                    "versions.yml:md5,80949a94ee1be8238b1c79d89ed9fa83",
+                    "versions.yml:md5,ddd810edeee1008ec55b628e3a9d6204"
                 ]
             }
         ],
@@ -75,6 +75,6 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-05-03T16:03:28.825915"
+        "timestamp": "2024-05-22T15:55:50.527259"
     }
 }
\ No newline at end of file

From eb3cf6fcc3ebf8d77fa6f988aa98570069cc21ec Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 22 May 2024 16:45:01 +1200
Subject: [PATCH 49/76] Added stub to merqury

---
 modules/nf-core/merqury/main.nf      | 26 +++++++++++++++++++++++
 modules/nf-core/merqury/merqury.diff | 31 ++++++++++++++++++++++++++++
 2 files changed, 57 insertions(+)

diff --git a/modules/nf-core/merqury/main.nf b/modules/nf-core/merqury/main.nf
index 196b0dfc..0e0d4369 100644
--- a/modules/nf-core/merqury/main.nf
+++ b/modules/nf-core/merqury/main.nf
@@ -57,4 +57,30 @@ process MERQURY {
         merqury: $VERSION
     END_VERSIONS
     """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = 1.3
+    """
+    touch ${prefix}_only.bed
+    touch ${prefix}_only.wig
+    touch ${prefix}.completeness.stats
+    touch ${prefix}.dist_only.hist
+    touch ${prefix}.spectra-cn.fl.png
+    touch ${prefix}.spectra-cn.hist
+    touch ${prefix}.spectra-cn.ln.png
+    touch ${prefix}.spectra-cn.st.png
+    touch ${prefix}.spectra-asm.fl.png
+    touch ${prefix}.spectra-asm.hist
+    touch ${prefix}.spectra-asm.ln.png
+    touch ${prefix}.spectra-asm.st.png
+    touch ${prefix}.qv
+    touch ${prefix}.${prefix}.qv
+    touch ${prefix}.hist.ploidy
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        merqury: $VERSION
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/merqury/merqury.diff b/modules/nf-core/merqury/merqury.diff
index d5ba84f6..e97121d7 100644
--- a/modules/nf-core/merqury/merqury.diff
+++ b/modules/nf-core/merqury/merqury.diff
@@ -9,5 +9,36 @@ Changes in module 'nf-core/merqury'
      path "versions.yml"                          , emit: versions
  
      when:
+@@ -56,4 +57,30 @@
+         merqury: $VERSION
+     END_VERSIONS
+     """
++
++    stub:
++    prefix = task.ext.prefix ?: "${meta.id}"
++    def VERSION = 1.3
++    """
++    touch ${prefix}_only.bed
++    touch ${prefix}_only.wig
++    touch ${prefix}.completeness.stats
++    touch ${prefix}.dist_only.hist
++    touch ${prefix}.spectra-cn.fl.png
++    touch ${prefix}.spectra-cn.hist
++    touch ${prefix}.spectra-cn.ln.png
++    touch ${prefix}.spectra-cn.st.png
++    touch ${prefix}.spectra-asm.fl.png
++    touch ${prefix}.spectra-asm.hist
++    touch ${prefix}.spectra-asm.ln.png
++    touch ${prefix}.spectra-asm.st.png
++    touch ${prefix}.qv
++    touch ${prefix}.${prefix}.qv
++    touch ${prefix}.hist.ploidy
++
++    cat <<-END_VERSIONS > versions.yml
++    "${task.process}":
++        merqury: $VERSION
++    END_VERSIONS
++    """
+ }
 
 ************************************************************

From 5ff445846b4595bbc3a4c735cb47dfa9cceef289 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 22 May 2024 22:13:19 +1200
Subject: [PATCH 50/76] Updated docs and removed meryl patches

---
 CHANGELOG.md                                  |  2 +-
 docs/output.md                                |  7 +-
 docs/usage.md                                 |  2 +
 modules.json                                  | 10 +--
 modules/nf-core/meryl/count/meryl-count.diff  | 79 -------------------
 .../meryl/unionsum/meryl-unionsum.diff        | 78 ------------------
 6 files changed, 9 insertions(+), 169 deletions(-)
 delete mode 100644 modules/nf-core/meryl/count/meryl-count.diff
 delete mode 100644 modules/nf-core/meryl/unionsum/meryl-unionsum.diff

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c340fc9e..7f7c1b0f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,7 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 12. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
 13. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
 14. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
-15. Locally patched `MERQURY`, `MERYL/COUNT` and `MERYL/UNIONSUM`
+15. Locally patched `MERQURY`
 
 ### `Fixed`
 
diff --git a/docs/output.md b/docs/output.md
index 15ee0559..8362c9c7 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -210,11 +210,8 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 <summary>Output files</summary>
 
 - `merqury/`
-  - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`.
-    - `*.completeness.stats`: Assembly completeness statistics
-    - `*.qv`: Assembly consensus quality QV statistics
-    - `*.*.spectra-cn.fl.png`: Spectra-cn plot
-    </details>
+  - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`. - `*.completeness.stats`: Assembly completeness statistics - `*.qv`: Assembly consensus quality QV statistics - `*.fl.png`: Spectra plots - `*.hapmers.blob.png`: Hap-mer blob plot
+  </details>
 
 [MERQURY](https://github.com/marbl/merqury) is used for the k-mer analysis.
 
diff --git a/docs/usage.md b/docs/usage.md
index 1d803b63..ca71fab5 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -103,6 +103,8 @@ See following assemblysheet examples for MERQURY analysis.
 - [assemblysheet - phased2x](../tests/merqury/phased2x/assemblysheet.csv)
 - [assemblysheet - phased2x with parent reads](../tests/merqury/phased2x.mp/assemblysheet.csv)
 
+The data for these examples comes from: [umd.edu](https://obj.umiacs.umd.edu/marbl_publications/triobinning/index.html)
+
 #### Parameters
 
 - `merqury_kmer_length`: kmer length for merqury analysis
diff --git a/modules.json b/modules.json
index b60aa0ff..8d914f44 100644
--- a/modules.json
+++ b/modules.json
@@ -163,15 +163,13 @@
                     },
                     "meryl/count": {
                         "branch": "master",
-                        "git_sha": "c95215baf20e6d8cf2ae0e8411d03c5d9ef21d4a",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/meryl/count/meryl-count.diff"
+                        "git_sha": "1c72d95476a2edf92ee4f08dc4321b3c50cd3c13",
+                        "installed_by": ["modules"]
                     },
                     "meryl/unionsum": {
                         "branch": "master",
-                        "git_sha": "b7f584e61c35c138a5717f837f4da9b838e290a7",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/meryl/unionsum/meryl-unionsum.diff"
+                        "git_sha": "1c72d95476a2edf92ee4f08dc4321b3c50cd3c13",
+                        "installed_by": ["modules"]
                     },
                     "seqkit/seq": {
                         "branch": "master",
diff --git a/modules/nf-core/meryl/count/meryl-count.diff b/modules/nf-core/meryl/count/meryl-count.diff
deleted file mode 100644
index 4bdabc19..00000000
--- a/modules/nf-core/meryl/count/meryl-count.diff
+++ /dev/null
@@ -1,79 +0,0 @@
-Changes in module 'nf-core/meryl/count'
---- modules/nf-core/meryl/count/meta.yml
-+++ modules/nf-core/meryl/count/meta.yml
-@@ -38,8 +38,9 @@
-   - meryl_db:
-       type: directory
-       description: A Meryl k-mer database
--      pattern: "*.meryldb"
-+      pattern: "*.meryl"
- authors:
-   - "@mahesh-panchal"
- maintainers:
-   - "@mahesh-panchal"
-+  - "@gallvp"
-
---- modules/nf-core/meryl/count/main.nf
-+++ modules/nf-core/meryl/count/main.nf
-@@ -12,8 +12,8 @@
-     val kvalue
- 
-     output:
--    tuple val(meta), path("*.meryldb"), emit: meryl_db
--    path "versions.yml"               , emit: versions
-+    tuple val(meta), path("*.meryl")    , emit: meryl_db
-+    path "versions.yml"                 , emit: versions
- 
-     when:
-     task.ext.when == null || task.ext.when
-@@ -26,9 +26,10 @@
-         meryl count \\
-             k=$kvalue \\
-             threads=$task.cpus \\
-+            memory=${task.memory.toGiga()} \\
-             $args \\
-             $reads \\
--            output read.\${READ%.f*}.meryldb
-+            output read.\${READ%.f*}.meryl
-     done
- 
-     cat <<-END_VERSIONS > versions.yml
-@@ -42,7 +43,7 @@
-     def prefix = task.ext.prefix ?: "${meta.id}"
-     """
-     for READ in $reads; do
--        touch read.\${READ%.f*}.meryldb
-+        touch read.\${READ%.f*}.meryl
-     done
- 
-     cat <<-END_VERSIONS > versions.yml
-
---- modules/nf-core/meryl/count/tests/main.nf.test.snap
-+++ modules/nf-core/meryl/count/tests/main.nf.test.snap
-@@ -303,7 +303,7 @@
-                             "id": "test",
-                             "single_end": true
-                         },
--                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        "read.test1_1.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
-                     ]
-                 ],
-                 "1": [
-@@ -315,7 +315,7 @@
-                             "id": "test",
-                             "single_end": true
-                         },
--                        "read.test1_1.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        "read.test1_1.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
-                     ]
-                 ],
-                 "versions": [
-@@ -327,6 +327,6 @@
-             "nf-test": "0.8.4",
-             "nextflow": "23.10.1"
-         },
--        "timestamp": "2024-03-26T19:30:13.745468405"
-+        "timestamp": "2024-05-22T12:27:16.300916"
-     }
- }
-************************************************************
diff --git a/modules/nf-core/meryl/unionsum/meryl-unionsum.diff b/modules/nf-core/meryl/unionsum/meryl-unionsum.diff
deleted file mode 100644
index 3a8058c1..00000000
--- a/modules/nf-core/meryl/unionsum/meryl-unionsum.diff
+++ /dev/null
@@ -1,78 +0,0 @@
-Changes in module 'nf-core/meryl/unionsum'
---- modules/nf-core/meryl/unionsum/meta.yml
-+++ modules/nf-core/meryl/unionsum/meta.yml
-@@ -36,8 +36,9 @@
-   - meryl_db:
-       type: directory
-       description: A Meryl k-mer database that is the union sum of the input databases
--      pattern: "*.unionsum.meryldb"
-+      pattern: "*.unionsum.meryl"
- authors:
-   - "@mahesh-panchal"
- maintainers:
-   - "@mahesh-panchal"
-+  - "@gallvp"
-
---- modules/nf-core/meryl/unionsum/main.nf
-+++ modules/nf-core/meryl/unionsum/main.nf
-@@ -12,7 +12,7 @@
-     val kvalue
- 
-     output:
--    tuple val(meta), path("*.unionsum.meryldb"), emit: meryl_db
-+    tuple val(meta), path("*.unionsum.meryl"), emit: meryl_db
-     path "versions.yml"                        , emit: versions
- 
-     when:
-@@ -25,8 +25,9 @@
-     meryl union-sum \\
-         k=$kvalue \\
-         threads=$task.cpus \\
-+        memory=${task.memory.toGiga()} \\
-         $args \\
--        output ${prefix}.unionsum.meryldb \\
-+        output ${prefix}.unionsum.meryl \\
-         $meryl_dbs
- 
-     cat <<-END_VERSIONS > versions.yml
-@@ -39,7 +40,7 @@
-     def args = task.ext.args ?: ''
-     def prefix = task.ext.prefix ?: "${meta.id}"
-     """
--    touch ${prefix}.unionsum.meryldb
-+    touch ${prefix}.unionsum.meryl
- 
-     cat <<-END_VERSIONS > versions.yml
-     "${task.process}":
-
---- modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
-+++ modules/nf-core/meryl/unionsum/tests/main.nf.test.snap
-@@ -8,7 +8,7 @@
-                             "id": "test",
-                             "single_end": true
-                         },
--                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        "test.unionsum.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
-                     ]
-                 ],
-                 "1": [
-@@ -20,7 +20,7 @@
-                             "id": "test",
-                             "single_end": true
-                         },
--                        "test.unionsum.meryldb:md5,d41d8cd98f00b204e9800998ecf8427e"
-+                        "test.unionsum.meryl:md5,d41d8cd98f00b204e9800998ecf8427e"
-                     ]
-                 ],
-                 "versions": [
-@@ -32,7 +32,7 @@
-             "nf-test": "0.8.4",
-             "nextflow": "23.10.1"
-         },
--        "timestamp": "2024-03-27T10:19:52.291287389"
-+        "timestamp": "2024-05-22T12:40:21.306142"
-     },
-     "sarscov2 - fastq - single_end": {
-         "content": [
-
-************************************************************

From c955ad40b2ca543531887fce35fa2f37e82e7b3a Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 27 May 2024 17:28:42 +1200
Subject: [PATCH 51/76] Added plotsr

---
 CHANGELOG.md                                  |   3 +
 CITATIONS.md                                  |  12 ++
 README.md                                     |  21 +-
 assets/xrefsheet.csv                          |   1 +
 bin/report_modules/parsers/synteny_parser.py  | 118 ++++++++++-
 bin/report_modules/templates/base.html        |  16 +-
 .../synteny_circos/synteny_circos.html        |   4 +-
 .../templates/synteny_dotplot/dropdown.html   |  10 +
 .../report_contents.html                      |  12 +-
 .../synteny_linear.html                       |   8 +-
 .../templates/synteny_linear/dropdown.html    |  10 -
 .../synteny_plotsr/report_contents.html       |  24 +++
 .../synteny_plotsr/synteny_plotsr.html        |  36 ++++
 conf/modules.config                           |  24 +++
 conf/test_full.config                         |   2 +
 docs/images/plotsr.png                        | Bin 0 -> 52708 bytes
 docs/output.md                                |  21 +-
 docs/parameters.md                            |  39 ++--
 docs/usage.md                                 |  35 +--
 modules.json                                  |  20 ++
 .../nf-core/minimap2/align/environment.yml    |   9 +
 modules/nf-core/minimap2/align/main.nf        |  63 ++++++
 modules/nf-core/minimap2/align/meta.yml       |  75 +++++++
 .../nf-core/minimap2/align/tests/main.nf.test | 181 ++++++++++++++++
 .../minimap2/align/tests/main.nf.test.snap    |  69 ++++++
 modules/nf-core/minimap2/align/tests/tags.yml |   2 +
 .../pfr/custom/relabelfasta/environment.yml   |  10 +
 modules/pfr/custom/relabelfasta/main.nf       |  40 ++++
 modules/pfr/custom/relabelfasta/meta.yml      |  56 +++++
 .../relabelfasta/templates/relabelfasta.py    |  92 ++++++++
 .../custom/relabelfasta/tests/main.nf.test    | 153 ++++++++++++++
 .../relabelfasta/tests/main.nf.test.snap      |  68 ++++++
 .../pfr/custom/relabelfasta/tests/tags.yml    |   2 +
 modules/pfr/plotsr/environment.yml            |   9 +
 modules/pfr/plotsr/main.nf                    |  91 ++++++++
 modules/pfr/plotsr/meta.yml                   |  79 +++++++
 modules/pfr/plotsr/tests/main.nf.test         | 121 +++++++++++
 modules/pfr/plotsr/tests/main.nf.test.snap    |  26 +++
 modules/pfr/plotsr/tests/nextflow.config      |   5 +
 modules/pfr/plotsr/tests/tags.yml             |   2 +
 modules/pfr/syri/environment.yml              |   9 +
 modules/pfr/syri/main.nf                      |  60 ++++++
 modules/pfr/syri/meta.yml                     |  64 ++++++
 modules/pfr/syri/tests/main.nf.test           |  80 +++++++
 modules/pfr/syri/tests/main.nf.test.snap      |  80 +++++++
 modules/pfr/syri/tests/nextflow.config        |   5 +
 modules/pfr/syri/tests/tags.yml               |   2 +
 nextflow.config                               |  14 +-
 nextflow_schema.json                          | 100 ++++++---
 pfr/params.json                               |   8 +-
 subworkflows/local/fasta_synteny.nf           | 199 ++++++++++++++++--
 workflows/assemblyqc.nf                       |  22 +-
 52 files changed, 2063 insertions(+), 149 deletions(-)
 create mode 100644 bin/report_modules/templates/synteny_dotplot/dropdown.html
 rename bin/report_modules/templates/{synteny_linear => synteny_dotplot}/report_contents.html (55%)
 rename bin/report_modules/templates/{synteny_linear => synteny_dotplot}/synteny_linear.html (80%)
 delete mode 100644 bin/report_modules/templates/synteny_linear/dropdown.html
 create mode 100644 bin/report_modules/templates/synteny_plotsr/report_contents.html
 create mode 100644 bin/report_modules/templates/synteny_plotsr/synteny_plotsr.html
 create mode 100644 docs/images/plotsr.png
 create mode 100644 modules/nf-core/minimap2/align/environment.yml
 create mode 100644 modules/nf-core/minimap2/align/main.nf
 create mode 100644 modules/nf-core/minimap2/align/meta.yml
 create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test
 create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/minimap2/align/tests/tags.yml
 create mode 100644 modules/pfr/custom/relabelfasta/environment.yml
 create mode 100644 modules/pfr/custom/relabelfasta/main.nf
 create mode 100644 modules/pfr/custom/relabelfasta/meta.yml
 create mode 100755 modules/pfr/custom/relabelfasta/templates/relabelfasta.py
 create mode 100644 modules/pfr/custom/relabelfasta/tests/main.nf.test
 create mode 100644 modules/pfr/custom/relabelfasta/tests/main.nf.test.snap
 create mode 100644 modules/pfr/custom/relabelfasta/tests/tags.yml
 create mode 100644 modules/pfr/plotsr/environment.yml
 create mode 100644 modules/pfr/plotsr/main.nf
 create mode 100644 modules/pfr/plotsr/meta.yml
 create mode 100644 modules/pfr/plotsr/tests/main.nf.test
 create mode 100644 modules/pfr/plotsr/tests/main.nf.test.snap
 create mode 100644 modules/pfr/plotsr/tests/nextflow.config
 create mode 100644 modules/pfr/plotsr/tests/tags.yml
 create mode 100644 modules/pfr/syri/environment.yml
 create mode 100644 modules/pfr/syri/main.nf
 create mode 100644 modules/pfr/syri/meta.yml
 create mode 100644 modules/pfr/syri/tests/main.nf.test
 create mode 100644 modules/pfr/syri/tests/main.nf.test.snap
 create mode 100644 modules/pfr/syri/tests/nextflow.config
 create mode 100644 modules/pfr/syri/tests/tags.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7f7c1b0f..9179f33a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,6 +22,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 13. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
 14. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
 15. Locally patched `MERQURY`
+16. Added PLOTSR [#77](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/77)
+17. Added [JADWOS01](https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_016859245.1/) assembly to xrefsheet for successfully running PLOTSR.
 
 ### `Fixed`
 
@@ -33,6 +35,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 6. `RUNASSEMBLYVISUALIZER` is now single threaded for successful execution on both Linux and MacOS
 7. Fixed java memory overflow issues in `RUNASSEMBLYVISUALIZER`
 8. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]` [#83](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/83)
+9. Improved input assembly documentation [#86](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/86)
 
 ### `Dependencies`
 
diff --git a/CITATIONS.md b/CITATIONS.md
index 6ee2a4f5..fbd8036d 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -116,6 +116,18 @@
 
   > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: <https://doi.org/10.1371/journal.pcbi.1005944>. PMID: 29373581; PMCID: PMC5802927.
 
+- PLOTSR, [MIT](https://github.com/schneebergerlab/plotsr/blob/master/LICENSE)
+
+  > Goel M, Schneeberger K. 2022. plotsr: visualizing structural similarities and rearrangements between multiple genomes. Bioinformatics. 2022 May 13;38(10):2922-2926. doi: <https://doi.org/10.1093/bioinformatics/btac196>. PMID: 35561173; PMCID: PMC9113368.
+
+- SYRI, [MIT](https://github.com/schneebergerlab/syri/blob/master/LICENSE)
+
+  > Goel M, Sun H, Jiao WB, Schneeberger K. 2019. SyRI: finding genomic rearrangements and local sequence differences from whole-genome assemblies. Genome Biol. 2019 Dec 16;20(1):277. doi: <https://doi.org/10.1186/s13059-019-1911-0>. PMID: 31842948; PMCID: PMC6913012.
+
+- MINIMAP2, [MIT](https://github.com/lh3/minimap2/blob/master/LICENSE.txt)
+
+  > Li H. 2021. New strategies to improve minimap2 alignment accuracy, Bioinformatics, Volume 37, Issue 23, December 2021, Pages 4572–4574, doi: <https://doi.org/10.1093/bioinformatics/btab705>
+
 - MERQURY, [United States Government Work](https://github.com/marbl/merqury?tab=License-1-ov-file#readme)
 
   > Rhie, A., Walenz, B.P., Koren, S. et al. 2020. Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies. Genome Biol 21, 245. doi: <https://doi.org/10.1186/s13059-020-02134-9>
diff --git a/README.md b/README.md
index 30ec1d32..60b05536 100644
--- a/README.md
+++ b/README.md
@@ -15,6 +15,11 @@
 ## Pipeline Flowchart
 
 ```mermaid
+%%{init: {
+  "themeVariables": {
+    "fontSize": "52px"
+  }
+}}%%
 flowchart LR
   forEachTag(For each\nAssembly) --> VALIDATE_FORMAT[VALIDATE FORMAT]
 
@@ -36,9 +41,15 @@ flowchart LR
   Run --> LAI
   Run --> KRAKEN2
   Run --> HIC_CONTACT_MAP[HIC CONTACT MAP]
-  Run --> SYNTENY
+  Run --> MUMMER
+  Run --> MINIMAP2
   Run --> MERQURY
 
+  MUMMER --> CIRCOS
+  MUMMER --> DOTPLOT
+
+  MINIMAP2 --> PLOTSR
+
   ASS_STATS --> REPORT
   GFF_STATS --> REPORT
   BUSCO --> REPORT
@@ -46,7 +57,9 @@ flowchart LR
   LAI --> REPORT
   KRAKEN2 --> REPORT
   HIC_CONTACT_MAP --> REPORT
-  SYNTENY --> REPORT
+  CIRCOS --> REPORT
+  DOTPLOT --> REPORT
+  PLOTSR --> REPORT
   MERQURY --> REPORT
 ```
 
@@ -61,7 +74,7 @@ flowchart LR
 - [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences
 - [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification
 - [HIC CONTACT MAP](https://github.com/igvteam/juicebox.js): Alignment and visualisation of HiC data
-- SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer), [CIRCOS](http://circos.ca/documentation/) and [PLOTLY](https://plotly.com)
+- [MUMMER](https://github.com/mummer4/mummer) → [CIRCOS](http://circos.ca/documentation/) + [DOTPLOT](https://plotly.com) & [MINIMAP2](https://github.com/lh3/minimap2) → [PLOTSR](https://github.com/schneebergerlab/plotsr): Synteny analysis
 - [MERQURY](https://github.com/marbl/merqury): K-mer completeness, consensus quality and phasing assessment
 
 ## Usage
@@ -105,7 +118,9 @@ We thank the following people for their extensive assistance in the development
 
 - Cecilia Deng [@CeciliaDeng](https://github.com/CeciliaDeng)
 - Chen Wu [@christinawu2008](https://github.com/christinawu2008)
+- Ignacio Carvajal [@ignacio3437](https://github.com/ignacio3437)
 - Jason Shiller [@jasonshiller](https://github.com/jasonshiller)
+- Mahesh Binzer-Panchal [@mahesh-panchal](https://github.com/mahesh-panchal)
 - Marcus Davy [@mdavy86](https://github.com/mdavy86)
 - Ross Crowhurst [@rosscrowhurst](https://github.com/rosscrowhurst)
 - Sarah Bailey [@SarahBailey1998](https://github.com/SarahBailey1998)
diff --git a/assets/xrefsheet.csv b/assets/xrefsheet.csv
index 5c218707..e945d3e4 100644
--- a/assets/xrefsheet.csv
+++ b/assets/xrefsheet.csv
@@ -1,2 +1,3 @@
 tag,fasta,synteny_labels
 TT_2021a,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/TT_2021a.seq.labels.tsv
+JAD,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/016/859/245/GCA_016859245.1_ASM1685924v1/GCA_016859245.1_ASM1685924v1_genomic.fna.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/JAD.seq.labels.tsv
diff --git a/bin/report_modules/parsers/synteny_parser.py b/bin/report_modules/parsers/synteny_parser.py
index 0f871114..d25d82e9 100644
--- a/bin/report_modules/parsers/synteny_parser.py
+++ b/bin/report_modules/parsers/synteny_parser.py
@@ -1,6 +1,8 @@
-import os
+from tabulate import tabulate
 from pathlib import Path
+import pandas as pd
 import base64
+import os
 import re
 
 from report_modules.parsers.parsing_commons import sort_list_of_results
@@ -18,9 +20,14 @@ def parse_synteny_circos(folder_name="synteny_outputs"):
     data = {"SYNTENY_CIRCOS": []}
 
     for plot_path in list_of_plot_files:
+        base_name = os.path.basename(str(plot_path))
+
+        if base_name == "plotsr.png":
+            continue
+
         file_tokens = re.findall(
             r"([\w]+).on.([\w]+).([\w]+).png",
-            os.path.basename(str(plot_path)),
+            base_name,
         )[0]
 
         if os.path.getsize(plot_path) == 0:
@@ -54,7 +61,7 @@ def parse_synteny_circos(folder_name="synteny_outputs"):
     }
 
 
-def parse_synteny_linear(folder_name="synteny_outputs"):
+def parse_synteny_dotplot(folder_name="synteny_outputs"):
     dir = os.getcwdb().decode()
     circos_folder_path = Path(f"{dir}/{folder_name}")
 
@@ -63,7 +70,7 @@ def parse_synteny_linear(folder_name="synteny_outputs"):
 
     list_of_plot_files = [item for item in circos_folder_path.glob("*.html")]
 
-    data = {"SYNTENY_LINEAR": []}
+    data = {"SYNTENY_DOTPLOT": []}
 
     for plot_path in list_of_plot_files:
         file_tokens = re.findall(
@@ -72,7 +79,7 @@ def parse_synteny_linear(folder_name="synteny_outputs"):
         )[0]
 
         if os.path.getsize(plot_path) == 0:
-            data["SYNTENY_LINEAR"].append(
+            data["SYNTENY_DOTPLOT"].append(
                 {
                     "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
                     "plot": "",
@@ -84,7 +91,7 @@ def parse_synteny_linear(folder_name="synteny_outputs"):
 
         plot_filename = os.path.basename(str(plot_path))
 
-        data["SYNTENY_LINEAR"].append(
+        data["SYNTENY_DOTPLOT"].append(
             {
                 "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}",
                 "plot": plot_filename,
@@ -93,16 +100,107 @@ def parse_synteny_linear(folder_name="synteny_outputs"):
             }
         )
 
-    if len(data["SYNTENY_LINEAR"]) < 1:
+    if len(data["SYNTENY_DOTPLOT"]) < 1:
         return {}
 
     return {
-        "SYNTENY_LINEAR": sort_list_of_results(data["SYNTENY_LINEAR"], "tag.on.tag")
+        "SYNTENY_DOTPLOT": sort_list_of_results(data["SYNTENY_DOTPLOT"], "tag.on.tag")
     }
 
 
+def parse_synteny_plotsr(folder_name="synteny_outputs"):
+    dir = os.getcwdb().decode()
+    plotsr_folder_path = Path(f"{dir}/{folder_name}")
+
+    if not os.path.exists(plotsr_folder_path):
+        return {}
+
+    list_of_error_files = [item for item in plotsr_folder_path.glob("*.error.log")]
+
+    data = {"SYNTENY_PLOTSR": []}
+
+    error_comparisons = []
+
+    for error_log_path in list_of_error_files:
+        base_name = os.path.basename(str(error_log_path))
+
+        file_tokens = re.findall(
+            r"([\w]+).on.([\w]+).error.log",
+            base_name,
+        )[0]
+
+        error_comparisons.append((file_tokens[0], file_tokens[1]))
+
+    plot_url = None
+    plotsr_png_path = Path(f"{dir}/{folder_name}/plotsr.png")
+    if os.path.exists(plotsr_png_path):
+        binary_fc = open(plotsr_png_path, "rb").read()
+        base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8")
+        ext = str(plotsr_png_path).split(".")[-1]
+        plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}"
+
+    if error_comparisons == [] and plot_url == None:
+        return {}
+
+    data["SYNTENY_PLOTSR"].append(
+        {
+            "error_message": (
+                None
+                if error_comparisons == []
+                else "<b>Note:</b> Syri failed to detect structural rearrangements for following comparisons: "
+                + ", ".join(
+                    [f"{target} with reference to {ref}" for (target, ref) in error_comparisons]
+                )
+                + '. This may be due to known Syri limitations. See: <a href="https://github.com/schneebergerlab/syri/tree/ebd0f832e0df33398306f1b65f86801090c1ed49#current-limitations" target="_blank">GitHub/Syri/Limitations</a>'
+            ),
+            "plotsr_png": plot_url,
+            "labels_table": None,
+            "labels_table_html": None,
+        }
+    )
+
+    if plot_url == None:
+        return data
+
+    list_of_label_files = [item for item in plotsr_folder_path.glob("*.plotsr.csv")]
+    labels_table = pd.DataFrame()
+
+    for labels_path in list_of_label_files:
+        base_name = os.path.basename(str(labels_path))
+
+        file_token = re.findall(
+            r"([\w]+).plotsr.csv",
+            base_name,
+        )[0]
+
+        _labels_table = pd.read_csv(labels_path, header=None, sep="\t")
+        _labels_table = _labels_table.set_axis([file_token, "Labels"], axis=1)
+        _labels_table = _labels_table[["Labels", file_token]]
+
+        if labels_table.empty:
+            labels_table = _labels_table
+            continue
+
+        labels_table = pd.concat([labels_table, _labels_table[[file_token]]], axis=1)
+
+    if labels_table.empty:
+        return data
+
+    data["SYNTENY_PLOTSR"][0]["labels_table"] = labels_table.to_dict("records")
+    data["SYNTENY_PLOTSR"][0]["labels_table_html"] = tabulate(
+        labels_table,
+        headers="keys",
+        tablefmt="html",
+        numalign="left",
+        showindex=False,
+    )
+
+    return data
+
+
 def parse_synteny_folder(folder_name="synteny_outputs"):
     circos_data = parse_synteny_circos(folder_name)
-    linear_data = parse_synteny_linear(folder_name)
+    dotplot_data = parse_synteny_dotplot(folder_name)
+    plotsr_data = parse_synteny_plotsr(folder_name)
 
-    return {**circos_data, **linear_data}
+    return {**circos_data, **dotplot_data, **plotsr_data}
diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html
index b570d8e5..11fe7d92 100644
--- a/bin/report_modules/templates/base.html
+++ b/bin/report_modules/templates/base.html
@@ -64,8 +64,12 @@
     <button class="tablinks" onclick="openTool(event, 'SYNTENY_CIRCOS')">SYNTENY CIRCOS</button>
     {% endif %}
 
-    {% if 'SYNTENY_LINEAR' in all_stats_dicts %}
-    <button class="tablinks" onclick="openTool(event, 'SYNTENY_LINEAR')">SYNTENY LINEAR</button>
+    {% if 'SYNTENY_DOTPLOT' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'SYNTENY_DOTPLOT')">SYNTENY DOTPLOT</button>
+    {% endif %}
+
+    {% if 'SYNTENY_PLOTSR' in all_stats_dicts %}
+    <button class="tablinks" onclick="openTool(event, 'SYNTENY_PLOTSR')">SYNTENY PLOTSR</button>
     {% endif %}
 
     {% if 'MERQURY' in all_stats_dicts %}
@@ -128,8 +132,12 @@
   {% include 'synteny_circos/synteny_circos.html' %}
   {% endif %}
 
-  {% if 'SYNTENY_LINEAR' in all_stats_dicts %}
-  {% include 'synteny_linear/synteny_linear.html' %}
+  {% if 'SYNTENY_DOTPLOT' in all_stats_dicts %}
+  {% include 'synteny_dotplot/synteny_dotplot.html' %}
+  {% endif %}
+
+  {% if 'SYNTENY_PLOTSR' in all_stats_dicts %}
+  {% include 'synteny_plotsr/synteny_plotsr.html' %}
   {% endif %}
 
   {% if 'MERQURY' in all_stats_dicts %}
diff --git a/bin/report_modules/templates/synteny_circos/synteny_circos.html b/bin/report_modules/templates/synteny_circos/synteny_circos.html
index 2950621a..d7547707 100644
--- a/bin/report_modules/templates/synteny_circos/synteny_circos.html
+++ b/bin/report_modules/templates/synteny_circos/synteny_circos.html
@@ -24,11 +24,11 @@
         <p class="section-para"><b>Notes:</b></p>
         <ul>
           <li>
-            Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny_max_gap'] }}bp have been
+            Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny_mummer_max_gap'] }}bp have been
             bundled together.
           </li>
           <li>
-            After bundling, any bundle smaller than {{ all_stats_dicts['PARAMS_DICT']['synteny_min_bundle_size'] }}bp has been filtered out.
+            After bundling, any bundle smaller than {{ all_stats_dicts['PARAMS_DICT']['synteny_mummer_min_bundle_size'] }}bp has been filtered out.
           </li>
           <li>
             The sequence labels shown on the plot are based on the labelling file provided to the pipeline. These labels may or may not be same as the sequence IDs in the corresponding FASTA files.
diff --git a/bin/report_modules/templates/synteny_dotplot/dropdown.html b/bin/report_modules/templates/synteny_dotplot/dropdown.html
new file mode 100644
index 00000000..6325dfec
--- /dev/null
+++ b/bin/report_modules/templates/synteny_dotplot/dropdown.html
@@ -0,0 +1,10 @@
+<div class="dropdown">
+  <div class="dropdown_content">
+      <select id="selector_SYNTENY_DOTPLOT" onchange="showContent('SYNTENY_DOTPLOT')">
+          {% set str_tag_on_tag = 'tag.on.tag' %} {% for item in range(all_stats_dicts["SYNTENY_DOTPLOT"]|length) %}
+          <option value="tabcontent_SYNTENY_DOTPLOT_{{all_stats_dicts['SYNTENY_DOTPLOT'][item]['tag.on.tag']}}">
+              {{ all_stats_dicts['SYNTENY_DOTPLOT'][item][str_tag_on_tag] }} {% endfor %}
+          </option>
+      </select>
+  </div>
+</div>
diff --git a/bin/report_modules/templates/synteny_linear/report_contents.html b/bin/report_modules/templates/synteny_dotplot/report_contents.html
similarity index 55%
rename from bin/report_modules/templates/synteny_linear/report_contents.html
rename to bin/report_modules/templates/synteny_dotplot/report_contents.html
index 069fa018..be95d31d 100644
--- a/bin/report_modules/templates/synteny_linear/report_contents.html
+++ b/bin/report_modules/templates/synteny_dotplot/report_contents.html
@@ -1,12 +1,12 @@
-{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["SYNTENY_LINEAR"]|length) %} {% set active_text
+{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["SYNTENY_DOTPLOT"]|length) %} {% set active_text
 = 'display: block' if vars.is_first else 'display: none' %}
-<div id="tabcontent_SYNTENY_LINEAR_{{ all_stats_dicts['SYNTENY_LINEAR'][item]['tag.on.tag'] }}"
-  class="tabcontent-SYNTENY_LINEAR" style="{{ active_text }}">
+<div id="tabcontent_SYNTENY_DOTPLOT_{{ all_stats_dicts['SYNTENY_DOTPLOT'][item]['tag.on.tag'] }}"
+  class="tabcontent-SYNTENY_DOTPLOT" style="{{ active_text }}">
   <div class="results-section">
     <div class="section-heading-wrapper">
-      <div class="section-heading">{{ all_stats_dicts['SYNTENY_LINEAR'][item]['tag.on.tag'] }}</div>
+      <div class="section-heading">{{ all_stats_dicts['SYNTENY_DOTPLOT'][item]['tag.on.tag'] }}</div>
     </div>
-    {% if all_stats_dicts['SYNTENY_LINEAR'][item]['is_plot_empty'] %}
+    {% if all_stats_dicts['SYNTENY_DOTPLOT'][item]['is_plot_empty'] %}
     <div class="section-para-wrapper">
       <p class="section-para">
         Either no alignments were detected between the target and reference assemblies, or all the alignment
@@ -16,7 +16,7 @@
     {% else %}
     <div class="iframe-wrapper">
       <iframe
-        src="./synteny/{{ all_stats_dicts['SYNTENY_LINEAR'][item]['plot_folder'] }}/{{ all_stats_dicts['SYNTENY_LINEAR'][item]['plot'] }}"
+        src="./synteny/{{ all_stats_dicts['SYNTENY_DOTPLOT'][item]['plot_folder'] }}/{{ all_stats_dicts['SYNTENY_DOTPLOT'][item]['plot'] }}"
         width="100%" height="100%"></iframe>
     </div>
     {% endif %}
diff --git a/bin/report_modules/templates/synteny_linear/synteny_linear.html b/bin/report_modules/templates/synteny_dotplot/synteny_linear.html
similarity index 80%
rename from bin/report_modules/templates/synteny_linear/synteny_linear.html
rename to bin/report_modules/templates/synteny_dotplot/synteny_linear.html
index 6be686e6..91e1bd83 100644
--- a/bin/report_modules/templates/synteny_linear/synteny_linear.html
+++ b/bin/report_modules/templates/synteny_dotplot/synteny_linear.html
@@ -1,4 +1,4 @@
-<div id="SYNTENY_LINEAR" class="tabcontent" style="display: none">
+<div id="SYNTENY_DOTPLOT" class="tabcontent" style="display: none">
   <div class="section-para-wrapper">
     <p class="section-para">The genome-wide alignments are performed with MUMMER.</p>
     <p class="section-para"><b>References:</b></p>
@@ -13,11 +13,11 @@
     <p class="section-para"><b>Notes:</b></p>
     <ul>
       <li>
-        Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny_max_gap'] }}bp have been
+        Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny_mummer_max_gap'] }}bp have been
         bundled together.
       </li>
       <li>
-        After bundling, any bundle smaller than {{ all_stats_dicts['PARAMS_DICT']['synteny_min_bundle_size'] }}bp has
+        After bundling, any bundle smaller than {{ all_stats_dicts['PARAMS_DICT']['synteny_mummer_min_bundle_size'] }}bp has
         been filtered out.
       </li>
       <li>
@@ -26,5 +26,5 @@
       </li>
     </ul>
   </div>
-  {% include 'synteny_linear/dropdown.html' %} {% include 'synteny_linear/report_contents.html' %}
+  {% include 'synteny_dotplot/dropdown.html' %} {% include 'synteny_dotplot/report_contents.html' %}
 </div>
diff --git a/bin/report_modules/templates/synteny_linear/dropdown.html b/bin/report_modules/templates/synteny_linear/dropdown.html
deleted file mode 100644
index cfdf3956..00000000
--- a/bin/report_modules/templates/synteny_linear/dropdown.html
+++ /dev/null
@@ -1,10 +0,0 @@
-<div class="dropdown">
-  <div class="dropdown_content">
-      <select id="selector_SYNTENY_LINEAR" onchange="showContent('SYNTENY_LINEAR')">
-          {% set str_tag_on_tag = 'tag.on.tag' %} {% for item in range(all_stats_dicts["SYNTENY_LINEAR"]|length) %}
-          <option value="tabcontent_SYNTENY_LINEAR_{{all_stats_dicts['SYNTENY_LINEAR'][item]['tag.on.tag']}}">
-              {{ all_stats_dicts['SYNTENY_LINEAR'][item][str_tag_on_tag] }} {% endfor %}
-          </option>
-      </select>
-  </div>
-</div>
diff --git a/bin/report_modules/templates/synteny_plotsr/report_contents.html b/bin/report_modules/templates/synteny_plotsr/report_contents.html
new file mode 100644
index 00000000..9606b270
--- /dev/null
+++ b/bin/report_modules/templates/synteny_plotsr/report_contents.html
@@ -0,0 +1,24 @@
+<div id="tabcontent_SYNTENY_PLOTSR" class="tabcontent-SYNTENY_PLOTSR" style="display: block">
+  <div class="results-section">
+    {% if all_stats_dicts['SYNTENY_PLOTSR'][0]['error_message'] is not none %}
+    <div class="section-para-wrapper">
+      <p class="section-para">
+        {{ all_stats_dicts['SYNTENY_PLOTSR'][0]['error_message'] }}
+      </p>
+    </div>
+    {% endif %}
+    {% if all_stats_dicts['SYNTENY_PLOTSR'][0]['plotsr_png'] is not none %}
+    <div class="image-wrapper">
+      <img src="{{ all_stats_dicts['SYNTENY_PLOTSR'][0]['plotsr_png'] }}" alt="" />
+    </div>
+    {% endif %}
+    {% if all_stats_dicts['SYNTENY_PLOTSR'][0]['labels_table_html'] is not none %}
+    <div class="section-para-wrapper">
+      <p class="section-para"><b>Sequence labels</b></p>
+    </div>
+    <div class="table-outer">
+      <div class="table-wrapper">{{ all_stats_dicts['SYNTENY_PLOTSR'][0]['labels_table_html'] }}</div>
+    </div>
+    {% endif %}
+  </div>
+</div>
diff --git a/bin/report_modules/templates/synteny_plotsr/synteny_plotsr.html b/bin/report_modules/templates/synteny_plotsr/synteny_plotsr.html
new file mode 100644
index 00000000..60060828
--- /dev/null
+++ b/bin/report_modules/templates/synteny_plotsr/synteny_plotsr.html
@@ -0,0 +1,36 @@
+<div id="SYNTENY_PLOTSR" class="tabcontent" style="display: none">
+  <div class="section-para-wrapper">
+    <p class="section-para">
+      Plotsr generates high-quality visualisation of synteny and structural rearrangements between multiple genomes. For
+      this, it uses the genomic structural annotations between multiple chromosome-level assemblies. The genome-wide
+      alignments are performed with Minimap2.
+    </p>
+    <p class="section-para"><b>References:</b></p>
+    <p class="section-para">
+      Goel M, Schneeberger K. 2022. plotsr: visualizing structural similarities and rearrangements between multiple
+      genomes. Bioinformatics. 2022 May 13;38(10):2922-2926. doi: <a
+        href="https://doi.org/10.1093/bioinformatics/btac196" target="_blank">10.1093/bioinformatics/btac196</a>.
+      PMID: 35561173; PMCID: PMC9113368.
+    </p>
+    <p class="section-para">
+      Goel M, Sun H, Jiao WB, Schneeberger K. 2019. SyRI: finding genomic rearrangements and local sequence differences
+      from whole-genome assemblies. Genome Biol. 2019 Dec 16;20(1):277. doi: <a
+        href="https://doi.org/10.1186/s13059-019-1911-0" target="_blank">10.1186/s13059-019-1911-0</a>. PMID: 31842948;
+      PMCID: PMC6913012.
+    </p>
+
+    <p class="section-para">
+      Li H. 2021. New strategies to improve minimap2 alignment accuracy, Bioinformatics, Volume 37, Issue 23, December
+      2021, Pages 4572–4574, doi: <a href="https://doi.org/10.1093/bioinformatics/btab705"
+        target="_blank">10.1093/bioinformatics/btab705</a>
+    </p>
+
+    <p class="section-para">
+      <b>Versions: {% if 'PLOTSR' in all_stats_dicts['VERSIONS'] %}{{ all_stats_dicts['VERSIONS']['PLOTSR']['plotsr'] }}
+        (PLOTSR), {% endif %}{{
+        all_stats_dicts['VERSIONS']['SYRI']['syri'] }} (SYRI), {{
+        all_stats_dicts['VERSIONS']['MINIMAP2_ALIGN']['minimap2'] }} (MINIMAP2)</b>
+    </p>
+  </div>
+  {% include 'synteny_plotsr/report_contents.html' %}
+</div>
diff --git a/conf/modules.config b/conf/modules.config
index 783e6650..d199e968 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -142,6 +142,30 @@ process {
         ]
     }
 
+    withName: '.*:FASTA_SYNTENY:CUSTOM_RELABELFASTA' {
+        ext.prefix = { "${meta.id}.plotsr" }
+    }
+
+    withName:  MINIMAP2_ALIGN {
+        ext.args = '-x asm5 --eqx'
+    }
+
+    withName: '.*:FASTA_SYNTENY:SYRI' {
+        publishDir = [
+            path: { "${params.outdir}/synteny/plotsr" },
+            mode: params.publish_dir_mode,
+            pattern: '*.error.log'
+        ]
+    }
+
+    withName: '.*:FASTA_SYNTENY:PLOTSR' {
+        publishDir = [
+            path: { "${params.outdir}/synteny/plotsr" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals("versions.yml") ? null : filename }
+        ]
+    }
+
     withName: FILTER_BY_LENGTH {
         ext.args = params.tidk_filter_by_size ? "-m ${params.tidk_filter_size_bp}" : ''
         ext.prefix = { "${meta.id}.filtered" }
diff --git a/conf/test_full.config b/conf/test_full.config
index e3c9b3c9..a8e663ab 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -38,6 +38,8 @@ params {
     hic                         = 'SRR8238190'
 
     synteny_skip                = false
+    synteny_mummer_skip         = false
+    synteny_plotsr_skip         = false
     synteny_xref_assemblies     = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv'
 
     merqury_skip                = false
diff --git a/docs/images/plotsr.png b/docs/images/plotsr.png
new file mode 100644
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zSK`|f2O^n9*aqCNc+OVn3<J@nps4s0#4Od~Zctd?umO4z)@}mLDr0ZX1t`J5!8gF`
z5Do{kx_n2S^mx~VEJNqjA_TPp>xd|?P|;wAh$g&@-)mW-k%1k^8n{-!Mn`8QxrI;{
zxoTXY(P$?@{=7j5TNTRMLjiTY!yZVpW0KxPM2{GOwUJ!B_%sM;IW;x%Xr3U2_$U;g
zz@g4omf$Pqn@Qt!fKe2puwdWfj^4W`weTPY8-e8BQj@Sc0gnxK5Zd8lDe~jUv^+Z4
zuAbmHkBew8($I*w^Fn6N?*~UqE2qR@lz9vY{=X(e6n}3BX9Mb?LB`G%l6X6IGtu5w
ztPqx;|EVo60l}MZ@dL!>z&VJgB1XyT4DSq7jixOP{Yf1TDa8v(Nl}RZB)v4G-wufc
z;wOfR+tiTMi%vn)^0M_EB_O`mu3by5pUK#?T;+ff&NirTakw=**a?eZvYMsKho!Lw
ziQOb0ds`z$b)5hfp+9MJNr;!-Wa7U6I>N%&K%N9-FtxIhBq>sRg0s03fSCnu)gm~U
zh44E@0)x*`)ZsnB#HV*#0pT~Y5bCj^p=T`<7%2$K(dkOE$kDY}^&#k}@zQg%j>++*
z#SZ6?S>D>x^HtQbJa8KxonDWaf~d^=Q?vfQ$)6L((cw;PoZn~KEpA&rOCBL<V$`oD
L7RI^TT_XPjuO6`@

literal 0
HcmV?d00001

diff --git a/docs/output.md b/docs/output.md
index 8362c9c7..ff7a724f 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -191,18 +191,23 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 
 - `synteny/`
   - `*.*.all/`: Synteny files corresponding to all contigs of the target assembly with respect to all contig of the reference assembly.
-    - `*.on.*.all.png/svg`: Synteny plot generated with CIRCOS.
-    - `*.on.*.all.png/svg`: Synteny plot generated with Plotly.
+    - `*.on.*.all.png/svg`: Synteny plot generated with CIRCOS
+    - `*.on.*.all.html`: Synteny dotplot generated with Plotly
     - `bundled.links.tsv`: Bundled links file generated with MUMMER, `MUMMER/dnadiff.pl` and [bundlelinks.py](../bin/bundlelinks.py).
     - `circos.conf`: CIRCOS configuration file used to generate the synteny plot.
     - `karyotype.tsv`: Karyotype TSV file used to generate the synteny plot.
   - `*.on.*.*`: Synteny files corresponding to a single contig of the target assembly with respect to all contigs of the reference assembly.
-  </details>
+  - `plotsr`: Plotsr files
+    - `*.error.log`: Error log for the failed Syri comparison
+    - `*.plotsr.csv`: CSV file listing sequence IDs and labels used by plotsr
+    - `plotsr.png`: Plotsr synteny plot
+    </details>
 
 [Circos](https://circos.ca) and linear synteny plots are created from genome-wide alignments performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl).
 
 <div align="center"><img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
-<div align="center"><img src="images/synteny_linear.png" alt="AssemblyQC - Linear synteny plot" width="50%"><hr><em>AssemblyQC - Linear synteny plot</em></div>
+<div align="center"><img src="images/synteny_linear.png" alt="AssemblyQC - Dotplot synteny plot" width="50%"><hr><em>AssemblyQC - Dotplot synteny plot</em></div>
+<div align="center"><img src="images/plotsr.png" alt="AssemblyQC - Plotsr synteny plot" width="50%"><hr><em>AssemblyQC - Plotsr synteny plot</em></div>
 
 ### Merqury
 
@@ -210,8 +215,12 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 <summary>Output files</summary>
 
 - `merqury/`
-  - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`. - `*.completeness.stats`: Assembly completeness statistics - `*.qv`: Assembly consensus quality QV statistics - `*.fl.png`: Spectra plots - `*.hapmers.blob.png`: Hap-mer blob plot
-  </details>
+  - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`.
+    - `*.completeness.stats`: Assembly completeness statistics
+    - `*.qv`: Assembly consensus quality QV statistics
+    - `*.fl.png`: Spectra plots
+    - `*.hapmers.blob.png`: Hap-mer blob plot
+    </details>
 
 [MERQURY](https://github.com/marbl/merqury) is used for the k-mer analysis.
 
diff --git a/docs/parameters.md b/docs/parameters.md
index 9c8506c2..07cf3d4c 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -38,12 +38,12 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## TIDK options
 
-| Parameter             | Description                                                                          | Type      | Default | Required | Hidden |
-| --------------------- | ------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
-| `tidk_skip`           | Skip telomere identification                                                         | `boolean` | True    |          |        |
-| `tidk_repeat_seq`     | Telomere repeat sequence. Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG | `string`  |         |          |        |
-| `tidk_filter_by_size` | Filter size in base-pairs                                                            | `boolean` |         |          |        |
-| `tidk_filter_size_bp` | Filter size in base-pairs                                                            | `integer` | 1000000 |          |        |
+| Parameter             | Description                                                                                                | Type      | Default | Required | Hidden |
+| --------------------- | ---------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `tidk_skip`           | Skip telomere identification                                                                               | `boolean` | True    |          |        |
+| `tidk_repeat_seq`     | Telomere repeat sequence. Typical values for plant: TTTAGGG, fungus, vertebrates: TTAGGG and Insect: TTAGG | `string`  |         |          |        |
+| `tidk_filter_by_size` | Filter assembly sequences smaller than the specified length                                                | `boolean` |         |          |        |
+| `tidk_filter_size_bp` | Filter size in base-pairs                                                                                  | `integer` | 1000000 |          |        |
 
 ## LAI options
 
@@ -69,17 +69,22 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 
 ## Synteny options
 
-| Parameter                          | Description                                                                                                        | Type      | Default | Required | Hidden |
-| ---------------------------------- | ------------------------------------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ |
-| `synteny_skip`                     | Skip synteny analysis                                                                                              | `boolean` | True    |          |        |
-| `synteny_plot_type`                | Synteny plot type: 'linear', 'circos', or 'both'                                                                   | `string`  | both    |          |        |
-| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies                                                        | `boolean` | True    |          |        |
-| `synteny_many_to_many_align`       | Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)                                        | `boolean` |         |          |        |
-| `synteny_max_gap`                  | Alignments within this distance are bundled together                                                               | `integer` | 1000000 |          |        |
-| `synteny_min_bundle_size`          | After bundling, any bundle smaller than this size is filtered out                                                  | `integer` | 1000    |          |        |
-| `synteny_plot_1_vs_all`            | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly | `boolean` |         |          |        |
-| `synteny_color_by_contig`          | Synteny plot is colored by contig                                                                                  | `boolean` | True    |          |        |
-| `synteny_xref_assemblies`          | Reference assemblies for synteny analysis                                                                          | `string`  |         |          |        |
+| Parameter                          | Description                                                                                                                                                                | Type      | Default | Required | Hidden |
+| ---------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `synteny_skip`                     | Skip synteny analysis                                                                                                                                                      | `boolean` | True    |          |        |
+| `synteny_mummer_skip`              | Skip Mummer-based synteny analysis                                                                                                                                         | `boolean` | True    |          |        |
+| `synteny_plotsr_skip`              | Skip plotsr-based synteny analysis                                                                                                                                         | `boolean` | True    |          |        |
+| `synteny_xref_assemblies`          | Reference assemblies for synteny analysis                                                                                                                                  | `string`  |         |          |        |
+| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies                                                                                                                | `boolean` | True    |          |        |
+| `synteny_mummer_plot_type`         | Synteny plot type from Mummer alignments: 'dotplot', 'circos', or 'both'                                                                                                   | `string`  | both    |          |        |
+| `synteny_mummer_m2m_align`         | Include Mummer alignment blocks with many-to-many mappings                                                                                                                 | `boolean` |         |          |        |
+| `synteny_mummer_max_gap`           | Mummer alignments within this distance are bundled together                                                                                                                | `integer` | 1000000 |          |        |
+| `synteny_mummer_min_bundle_size`   | After bundling, any Mummer alignment bundle smaller than this size is filtered out                                                                                         | `integer` | 1000    |          |        |
+| `synteny_plot_1_vs_all`            | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly. This only applies to Mummer plots                      | `boolean` |         |          |        |
+| `synteny_color_by_contig`          | Mummer synteny plots are colored by contig. Otherwise, they are colored by bundle size                                                                                     | `boolean` | True    |          |        |
+| `synteny_plotsr_seq_label`         | Sequence label prefix for plotsr synteny                                                                                                                                   | `string`  | Chr     |          |        |
+| `synteny_plotsr_assembly_order`    | The order in which the assemblies should be compared, provided as space separated string of assembly tags. If absent, assemblies are ordered by their tags alphabetically. | `string`  |
+|                                    |                                                                                                                                                                            |           |
 
 ## Merqury options
 
diff --git a/docs/usage.md b/docs/usage.md
index ca71fab5..b7319586 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -7,8 +7,8 @@ You will need to create an assemblysheet with information about the assemblies y
 - `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report
 - `fasta:` FASTA file
 - `gff3 [Optional]:` GFF3 annotation file if available
-- `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
-- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) when performing synteny analysis
+- `monoploid_ids [Optional]:` A txt file listing the sequence IDs used to calculate LAI in monoploid mode if necessary. If the intent is to run LAI against all the sequences in an assembly, this file can be skipped for that assembly.
+- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence IDs (first column) and their labels for the synteny plots (second column) when performing synteny analysis. If a sequence ID is missing from this file, the corresponding sequence is excluded from the analysis.
 
 See the [Merqury](#merqury-k-mer-analysis) section For description of assemblysheet columns related to k-mer analysis with Merqury.
 
@@ -40,54 +40,41 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p
 
 ## Other parameters
 
+This section provides additional information for parameters. It does not list all the pipeline parameters. For an exhaustive list, see [parameters.md](./parameters.md).
+
 ### Assemblathon stats
 
 `assemblathon_stats_n_limit` is the number of 'N's for the unknown gap size. This number is used to split the scaffolds into contigs to compute contig-related stats. NCBI's recommendation for unknown gap size is 100 <https://www.ncbi.nlm.nih.gov/genbank/wgs_gapped/>.
 
-### NCBI FCS adaptor
-
-`ncbi_fcs_adaptor_empire` should be set to `euk` for Eukaryotes and `prok` for Prokaryotes.
-
 ### NCBI FCS GX
 
 - `ncbi_fcs_gx_tax_id` is the taxonomy ID for all the assemblies listed in the assemblysheet. A taxonomy ID can be obtained by searching a _Genus species_ at <https://www.ncbi.nlm.nih.gov/taxonomy>.
-- `contamination_stops_pipeline` when set to `true`, skips the remaining QC steps for an assembly which has adaptor or GX contamination.
 
 ### BUSCO
 
-- `busco_mode`: `geno` or `genome` for genome assemblies (DNA), `tran` or `transcriptome` for transcriptome assemblies (DNA), and `prot` or `proteins` for annotated gene sets (protein).
 - `busco_lineage_datasets`: A space-separated list of BUSCO lineages. Any number of lineages can be specified such as "fungi_odb10 hypocreales_odb10". Each assembly is assessed against each of the listed lineage. To select a lineage, refer to <https://busco.ezlab.org/list_of_lineages.html>.
 
 ### TIDK
 
 - `tidk_repeat_seq`: The telomere search sequence. To select an appropriate sequence, see <https://github.com/tolkit/a-telomeric-repeat-database>. Commonly used sequences are TTTAGGG (Plant), TTAGGG (Fungus, Vertebrates) and TTAGG (Insect). Further reading: <https://pubmed.ncbi.nlm.nih.gov/32153618>
-- `tidk_filter_by_size`: Set this flag to `true` to filter out assembly sequences smaller than the size specified by the next parameter (default: `false`).
-- `tidk_filter_size_bp`: Minimum size of the assembly sequence processed by TIDK (default: 1000000 (1Mbp)).
 
 ### HiC
 
-- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq|fq).gz'. These reads are applied to each assembly listed by assemblysheet.
-- `hic_skip_fastp`: Skip fastp trimming
-- `hic_skip_fastqc`: Skip QC by fastqc
-- `hic_fastp_ext_args`: Additional arguments for fastp (default: '--qualified_quality_phred 20 --length_required 50')
+- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq|fq).gz'. These reads are applied to each assembly listed by `input`.
 
 ### Synteny analysis
 
-- `synteny_plot_type`: Set it to `linear`, `circos`, or `both` to generate CIRCOS or linear or both synteny plots.
-- `synteny_between_input_assemblies`: Set it to `true` to create synteny plots between each pair of input assemblies. Default is `true`.
-- `synteny_many_to_many_align`: Set it to `true` to include alignment blocks with many-to-many mappings or set to `false` to only include 1-to-1 mappings. Default is `false`. See the documentation of `dnadiff` for further details: <https://github.com/mummer4/mummer/blob/master/docs/dnadiff.README>
-- `synteny_max_gap`: Alignments within this distance are bundled together. Default: 1000000 (1 Mbp).
-- `synteny_min_bundle_size`: After bundling, any bundle smaller than this size is filtered out. Default: 1000 (1 Kbp)
-- `synteny_plot_1_vs_all`: Set it to `true` to create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly. Set it to `false` to create a single plot for each target assembly against each reference assembly. This joint plot is also created when `plot_1_vs_all` is set to `true`. Default: `false`
-- `synteny_color_by_contig`: Set it to `true` to color the synteny plot by contig. Set it to `false` to color the synteny plot by the number of links in a bundle. Default: `true`
 - `synteny_xref_assemblies`: Similar to `--input`, this parameter also provides a CSV sheet listing external reference assemblies which are included in the synteny analysis but are not analysed by other QC tools. See the [example xrefsheet](../assets/xrefsheet.csv) included with the pipeline. Its fields are:
+
   - `tag:` A unique tag which represents the reference assembly in the final report
   - `fasta:` FASTA file
   - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column)
 
+- `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom.
+
 ### Merqury K-mer analysis
 
-#### Additional assemblysheet columns
+Additional assemblysheet columns:
 
 - `reads_1 [Optional]`: A SRA ID for paired FASTQ files or FASTA/FASTQ file path to assembly reads. The reads are used by [MERQURY](https://github.com/marbl/merqury) for k-mer analysis. If two assemblies have the same SRA ID or file path for `reads_1`, they are treated as haplotypes of the same genome by MERQURY. A genome can only have one or two haplotypes.
 - `reads_2 [Optional]`: This column lists the second file if paired reads are used. If `reads_1` is a SRA ID, this column is ignored.
@@ -105,10 +92,6 @@ See following assemblysheet examples for MERQURY analysis.
 
 The data for these examples comes from: [umd.edu](https://obj.umiacs.umd.edu/marbl_publications/triobinning/index.html)
 
-#### Parameters
-
-- `merqury_kmer_length`: kmer length for merqury analysis
-
 ## Running the pipeline
 
 The typical command for running the pipeline is as follows:
diff --git a/modules.json b/modules.json
index 8d914f44..2e69aedd 100644
--- a/modules.json
+++ b/modules.json
@@ -30,6 +30,11 @@
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
+                    "custom/relabelfasta": {
+                        "branch": "main",
+                        "git_sha": "0421bdeb1d7a71dce12e0af7dc18aea26e548074",
+                        "installed_by": ["modules"]
+                    },
                     "custom/restoregffids": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
@@ -85,6 +90,11 @@
                         "git_sha": "46b70684b6ae0ac60cae154c61796b293707db64",
                         "installed_by": ["modules"]
                     },
+                    "plotsr": {
+                        "branch": "main",
+                        "git_sha": "0413d9b25b127f1c705349fb42de8ff54e83e1e9",
+                        "installed_by": ["modules"]
+                    },
                     "samblaster": {
                         "branch": "main",
                         "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
@@ -94,6 +104,11 @@
                         "branch": "main",
                         "git_sha": "84f31d4be03e2ae6bee8e0b9fdea466be8e997bb",
                         "installed_by": ["gff3_gt_gff3_gff3validator_stat"]
+                    },
+                    "syri": {
+                        "branch": "main",
+                        "git_sha": "295e1d8666c746cb05e79563393035e81c9739fc",
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -171,6 +186,11 @@
                         "git_sha": "1c72d95476a2edf92ee4f08dc4321b3c50cd3c13",
                         "installed_by": ["modules"]
                     },
+                    "minimap2/align": {
+                        "branch": "master",
+                        "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5",
+                        "installed_by": ["modules"]
+                    },
                     "seqkit/seq": {
                         "branch": "master",
                         "git_sha": "2be41ca2cc780eca4293d1b0dd3850b0b7ac40a3",
diff --git a/modules/nf-core/minimap2/align/environment.yml b/modules/nf-core/minimap2/align/environment.yml
new file mode 100644
index 00000000..051ca8ef
--- /dev/null
+++ b/modules/nf-core/minimap2/align/environment.yml
@@ -0,0 +1,9 @@
+name: minimap2_align
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::minimap2=2.28
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf
new file mode 100644
index 00000000..62349edc
--- /dev/null
+++ b/modules/nf-core/minimap2/align/main.nf
@@ -0,0 +1,63 @@
+process MINIMAP2_ALIGN {
+    tag "$meta.id"
+    label 'process_high'
+
+    // Note: the versions here need to match the versions used in the mulled container below and minimap2/index
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3a70f8bc7e17b723591f6132418640cfdbc88246-0' :
+        'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3a70f8bc7e17b723591f6132418640cfdbc88246-0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    tuple val(meta2), path(reference)
+    val bam_format
+    val cigar_paf_format
+    val cigar_bam
+
+    output:
+    tuple val(meta), path("*.paf"), optional: true, emit: paf
+    tuple val(meta), path("*.bam"), optional: true, emit: bam
+    tuple val(meta), path("*.csi"), optional: true, emit: csi
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args  = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus-1} -o ${prefix}.bam ${args2}" : "-o ${prefix}.paf"
+    def cigar_paf = cigar_paf_format && !bam_format ? "-c" : ''
+    def set_cigar_bam = cigar_bam && bam_format ? "-L" : ''
+    """
+    minimap2 \\
+        $args \\
+        -t $task.cpus \\
+        ${reference ?: reads} \\
+        $reads \\
+        $cigar_paf \\
+        $set_cigar_bam \\
+        $bam_output
+
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def output_file = bam_format ? "${prefix}.bam" : "${prefix}.paf"
+    """
+    touch $output_file
+    touch ${prefix}.csi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml
new file mode 100644
index 00000000..408522d5
--- /dev/null
+++ b/modules/nf-core/minimap2/align/meta.yml
@@ -0,0 +1,75 @@
+name: minimap2_align
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - align
+  - fasta
+  - fastq
+  - genome
+  - paf
+  - reference
+tools:
+  - minimap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lh3/minimap2
+      documentation: https://github.com/lh3/minimap2#uguide
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FASTA or FASTQ files of size 1 and 2 for single-end
+        and paired-end data, respectively.
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test_ref']
+  - reference:
+      type: file
+      description: |
+        Reference database in FASTA format.
+  - bam_format:
+      type: boolean
+      description: Specify that output should be in BAM format
+  - cigar_paf_format:
+      type: boolean
+      description: Specify that output CIGAR should be in PAF format
+  - cigar_bam:
+      type: boolean
+      description: |
+        Write CIGAR with >65535 ops at the CG tag. This is recommended when
+        doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - paf:
+      type: file
+      description: Alignment in PAF format
+      pattern: "*.paf"
+  - bam:
+      type: file
+      description: Alignment in BAM format
+      pattern: "*.bam"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@heuermh"
+  - "@sofstam"
+  - "@sateeshperi"
+  - "@jfy133"
+maintainers:
+  - "@heuermh"
+  - "@sofstam"
+  - "@sateeshperi"
+  - "@jfy133"
diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test b/modules/nf-core/minimap2/align/tests/main.nf.test
new file mode 100644
index 00000000..83cceeab
--- /dev/null
+++ b/modules/nf-core/minimap2/align/tests/main.nf.test
@@ -0,0 +1,181 @@
+nextflow_process {
+
+    name "Test Process MINIMAP2_ALIGN"
+    script "../main.nf"
+    process "MINIMAP2_ALIGN"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "minimap2"
+    tag "minimap2/align"
+
+    test("sarscov2 - fastq, fasta, true, false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - [fastq1, fastq2], fasta, true, false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, [], true, false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    []
+                ]
+                input[2] = true
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, fasta, true, false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, fasta, false, false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[2] = false
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.paf[0][1]).name,
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap
new file mode 100644
index 00000000..19a8f204
--- /dev/null
+++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap
@@ -0,0 +1,69 @@
+{
+    "sarscov2 - fastq, fasta, true, false, false": {
+        "content": [
+            "test.bam",
+            [
+                "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-05T10:14:18.939731126"
+    },
+    "sarscov2 - fastq, fasta, true, false, false - stub": {
+        "content": [
+            "test.bam",
+            "test.csi",
+            [
+                "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-05T10:14:34.275879844"
+    },
+    "sarscov2 - fastq, fasta, false, false, false - stub": {
+        "content": [
+            "test.paf",
+            "test.csi",
+            [
+                "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-05T10:14:39.227958138"
+    },
+    "sarscov2 - [fastq1, fastq2], fasta, true, false, false": {
+        "content": [
+            "test.bam",
+            [
+                "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-05T10:14:24.265054877"
+    },
+    "sarscov2 - fastq, [], true, false, false": {
+        "content": [
+            "test.bam",
+            [
+                "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-05T10:14:29.27901773"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/minimap2/align/tests/tags.yml b/modules/nf-core/minimap2/align/tests/tags.yml
new file mode 100644
index 00000000..39dba374
--- /dev/null
+++ b/modules/nf-core/minimap2/align/tests/tags.yml
@@ -0,0 +1,2 @@
+minimap2/align:
+  - "modules/nf-core/minimap2/align/**"
diff --git a/modules/pfr/custom/relabelfasta/environment.yml b/modules/pfr/custom/relabelfasta/environment.yml
new file mode 100644
index 00000000..c45ae6ad
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/environment.yml
@@ -0,0 +1,10 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "custom_relabelfasta"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - biopython==1.75
+  - python==3.8.13
diff --git a/modules/pfr/custom/relabelfasta/main.nf b/modules/pfr/custom/relabelfasta/main.nf
new file mode 100644
index 00000000..6b851a83
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/main.nf
@@ -0,0 +1,40 @@
+process CUSTOM_RELABELFASTA {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/biopython:1.75':
+        'biocontainers/biopython:1.75' }"
+
+    input:
+    tuple val(meta), path(fasta)
+    path(labels)
+
+    output:
+    tuple val(meta), path("*.fasta")    , emit: fasta
+    path "versions.yml"                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args    = task.ext.args ?: ''
+    prefix      = task.ext.prefix ?: "${meta.id}"
+    if ( "${prefix}.fasta" == "$fasta" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    template 'relabelfasta.py'
+
+    stub:
+    def args    = task.ext.args ?: ''
+    def prefix  = task.ext.prefix ?: "${meta.id}"
+    if ( "${prefix}.fasta" == "$fasta" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    touch ${prefix}.fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | cut -d' ' -f2)
+        biopython: \$(pip list | grep "biopython" | cut -d' ' -f3)
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/custom/relabelfasta/meta.yml b/modules/pfr/custom/relabelfasta/meta.yml
new file mode 100644
index 00000000..7935872a
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/meta.yml
@@ -0,0 +1,56 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "custom_relabelfasta"
+description: |
+  Relabels IDs in a fasta file based on a TSV label list
+  consisting of original (first column) and new IDs (second column)
+keywords:
+  - genomics
+  - fasta
+  - relabel
+  - id
+  - rename
+  - sequence
+tools:
+  - "python":
+      description: |
+        Python is a programming language that lets you work quickly
+        and integrate systems more effectively
+      homepage: "https://www.python.org"
+      documentation: "https://docs.python.org/3/"
+      tool_dev_url: "https://github.com/python/cpython"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - fasta:
+      type: file
+      description: Input fasta file
+      pattern: "*.fasta"
+  - labels:
+      type: file
+      description: |
+        A TSV file with original (first column) and new ids (second column)
+      pattern: "*.tsv"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fasta:
+      type: file
+      description: Output fasta file
+      pattern: "*.fasta"
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/modules/pfr/custom/relabelfasta/templates/relabelfasta.py b/modules/pfr/custom/relabelfasta/templates/relabelfasta.py
new file mode 100755
index 00000000..039941c6
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/templates/relabelfasta.py
@@ -0,0 +1,92 @@
+#!/usr/bin/env python3
+
+import re
+from importlib.metadata import version
+from platform import python_version
+
+from Bio import SeqIO
+
+# The input fasta file path
+fasta_file_path = "$fasta"
+labels_file_path = "$labels"
+output_prefix = "$prefix"
+
+
+def create_name_mapping_from_tsv(file_path):
+    dictionary = {}
+
+    with open(file_path) as tsv_file:
+        for line in tsv_file:
+            columns = line.strip().split("\\t")
+            if len(columns) != 2:
+                raise ValueError(f"{file_path} should be a two column TSV file")
+
+            orig_id, new_id = columns[0], columns[1]
+
+            if orig_id in dictionary.keys():
+                raise ValueError(
+                    f"{orig_id} is repeated in {file_path}. Each sequence ID should be unique"
+                )
+
+            if new_id in dictionary.values():
+                raise ValueError(
+                    f"{new_id} is repeated in {file_path}. Each sequence label should be unique"
+                )
+
+            dictionary[orig_id] = new_id
+
+    return dictionary
+
+
+def write_fasta_with_new_ids(fasta_file_path, orig_to_new_id_map, file_prefix):
+    old_fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta")
+
+    replaced_records = []
+    for record in old_fasta_file_obj:
+        orig_id = record.id
+
+        if orig_id not in orig_to_new_id_map.keys():
+            continue
+
+        new_id = orig_to_new_id_map[orig_id]
+        record.id = new_id
+        record.description = ""
+
+        replaced_records.append(record)
+
+    if replaced_records == []:
+        raise ValueError(
+            f"None of the sequences in {fasta_file_path} are selected by the input label list {orig_to_new_id_map}"
+        )
+
+    selected_ids = [record.id for record in replaced_records]
+    missing_ids = [
+        orig_id
+        for (orig_id, new_id) in orig_to_new_id_map.items()
+        if new_id not in selected_ids
+    ]
+
+    if len(missing_ids) > 0:
+        raise ValueError(
+            f"Some sequences {missing_ids} are missing from the fasta {fasta_file_path}"
+        )
+
+    SeqIO.write(replaced_records, f"{file_prefix}.fasta", "fasta")
+
+
+def match_substrings(substrings, target_string):
+    pattern = "|".join(map(re.escape, substrings))
+    matches = re.findall(pattern, target_string)
+    return matches
+
+
+if __name__ == "__main__":
+    orig_to_new_id_map = create_name_mapping_from_tsv(labels_file_path)
+
+    # Write versions
+    with open("versions.yml", "w") as f_versions:
+        f_versions.write('"${task.process}":\\n')
+        f_versions.write(f"    python: {python_version()}\\n")
+        f_versions.write(f"    biopython: {version('biopython')}\\n")
+
+    write_fasta_with_new_ids(fasta_file_path, orig_to_new_id_map, output_prefix)
diff --git a/modules/pfr/custom/relabelfasta/tests/main.nf.test b/modules/pfr/custom/relabelfasta/tests/main.nf.test
new file mode 100644
index 00000000..64ccb08f
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/tests/main.nf.test
@@ -0,0 +1,153 @@
+nextflow_process {
+
+    name "Test Process CUSTOM_RELABELFASTA"
+    script "../main.nf"
+    process "CUSTOM_RELABELFASTA"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "custom"
+    tag "custom/relabelfasta"
+
+    test("sarscov2 - fasta") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = Channel.of('MT192765.1\tChr1').collectFile(name: 'id_map.tsv', newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+
+            )
+        }
+
+    }
+
+    test("sarscov2 - fasta - repeated labels") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = Channel.of('MT192765.1\\tChr1\\nMT19276\\tChr1').collectFile(name: 'id_map.tsv', newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert ! process.success },
+                { assert process.stdout.toString().contains('Each sequence label should be unique') }
+
+            )
+        }
+
+    }
+
+    test("sarscov2 - fasta - repeated ids") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = Channel.of('MT192765.1\\tChr1\\nMT192765.1\\tChr1').collectFile(name: 'id_map.tsv', newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert ! process.success },
+                { assert process.stdout.toString().contains('Each sequence ID should be unique') }
+
+            )
+        }
+
+    }
+
+    test("sarscov2 - fasta - malformed") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = Channel.of('MT192765.1\\tChr1\\nMT192765.1').collectFile(name: 'id_map.tsv', newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert ! process.success },
+                { assert process.stdout.toString().contains('should be a two column TSV file') }
+
+            )
+        }
+
+    }
+
+    test("sarscov2 - fasta - missing ids") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = Channel.of('MT192765.1\\tChr1\\nMT192765.2\\tChr2').collectFile(name: 'id_map.tsv', newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert ! process.success },
+                { assert process.stdout.toString().contains('missing from the fasta') }
+
+            )
+        }
+
+    }
+
+    test("sarscov2 - fasta - stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = Channel.of('MT192765.1\tChr1').collectFile(name: 'id_map.tsv', newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+
+            )
+        }
+
+    }
+
+}
diff --git a/modules/pfr/custom/relabelfasta/tests/main.nf.test.snap b/modules/pfr/custom/relabelfasta/tests/main.nf.test.snap
new file mode 100644
index 00000000..6b1b4ff2
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/tests/main.nf.test.snap
@@ -0,0 +1,68 @@
+{
+    "sarscov2 - fasta - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d2052e0039190dca1b50700028ef14c3"
+                ],
+                "fasta": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2052e0039190dca1b50700028ef14c3"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-25T13:40:44.896721"
+    },
+    "sarscov2 - fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,393572534251b71ec74fbe3b1a31a709"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d2052e0039190dca1b50700028ef14c3"
+                ],
+                "fasta": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,393572534251b71ec74fbe3b1a31a709"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2052e0039190dca1b50700028ef14c3"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-25T13:38:20.323976"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/custom/relabelfasta/tests/tags.yml b/modules/pfr/custom/relabelfasta/tests/tags.yml
new file mode 100644
index 00000000..2db1f658
--- /dev/null
+++ b/modules/pfr/custom/relabelfasta/tests/tags.yml
@@ -0,0 +1,2 @@
+custom/relabelfasta:
+  - "modules/pfr/custom/relabelfasta/**"
diff --git a/modules/pfr/plotsr/environment.yml b/modules/pfr/plotsr/environment.yml
new file mode 100644
index 00000000..39f05f88
--- /dev/null
+++ b/modules/pfr/plotsr/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "plotsr"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - "bioconda::plotsr=1.1.1"
diff --git a/modules/pfr/plotsr/main.nf b/modules/pfr/plotsr/main.nf
new file mode 100644
index 00000000..664c1a24
--- /dev/null
+++ b/modules/pfr/plotsr/main.nf
@@ -0,0 +1,91 @@
+process PLOTSR {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/plotsr:1.1.1--pyh7cba7a3_0':
+        'biocontainers/plotsr:1.1.1--pyh7cba7a3_0' }"
+
+    input:
+    tuple val(meta), path(syri)
+    path(fastas)
+    val(genomes)
+    path(bedpe)
+    path(markers)
+    path(tracks)
+    path(chrord)
+    path(chrname)
+
+    output:
+    tuple val(meta), path("*.png"), emit: png
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args        = task.ext.args ?: ''
+    def prefix      = task.ext.prefix ?: "${meta.id}"
+    def syri_arg    = ( syri instanceof List )  ? syri.collect { "--sr $it" }.join(' ') : "--sr $syri"
+    def genomes_txt = genomes.tokenize('\n').withIndex().collect { line, index -> index == 0 ? line : "${fastas[index-1]}\t${line}" }.join('\\n')
+    def bedpe_arg   = bedpe     ? "--bedpe $bedpe"      : ''
+    def markers_arg = markers   ? "--markers $markers"  : ''
+    def tracks_arg  = tracks    ? "--tracks $tracks"    : ''
+    def chrord_arg  = chrord    ? "--chrord $chrord"    : ''
+    def chrname_arg = chrname   ? "--chrname $chrname"  : ''
+    """
+    echo -e "${genomes_txt}" \\
+        > ${prefix}.genomes.txt
+
+    plotsr \\
+        $syri_arg \\
+        --genomes ${prefix}.genomes.txt \\
+        $bedpe_arg \\
+        $markers_arg \\
+        $tracks_arg \\
+        $chrord_arg \\
+        $chrname_arg \\
+        $args \\
+        -o ${prefix}.png
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        plotsr: \$(plotsr --version)
+    END_VERSIONS
+    """
+
+    stub:
+    def args        = task.ext.args ?: ''
+    def prefix      = task.ext.prefix ?: "${meta.id}"
+    def syri_arg    = ( syri instanceof List )  ? syri.collect { "--sr $it" }.join(' ') : "--sr $syri"
+    def genomes_txt = genomes.tokenize('\n').withIndex().collect { line, index -> index == 0 ? line : "${fastas[index-1]}\t${line}" }.join('\\n')
+    def bedpe_arg   = bedpe     ? "--bedpe $bedpe"      : ''
+    def markers_arg = markers   ? "--markers $markers"  : ''
+    def tracks_arg  = tracks    ? "--tracks $tracks"    : ''
+    def chrord_arg  = chrord    ? "--chrord $chrord"    : ''
+    def chrname_arg = chrname   ? "--chrname $chrname"  : ''
+    """
+    echo -e "${genomes_txt}" \\
+        > ${prefix}.genomes.txt
+
+    echo \\
+    "plotsr \\
+        $syri_arg \\
+        --genomes ${prefix}.genomes.txt \\
+        $bedpe_arg \\
+        $markers_arg \\
+        $tracks_arg \\
+        $chrord_arg \\
+        $chrname_arg \\
+        $args \\
+        -o ${prefix}.png"
+
+    touch ${prefix}.png
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        plotsr: \$(plotsr --version)
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/plotsr/meta.yml b/modules/pfr/plotsr/meta.yml
new file mode 100644
index 00000000..c728b683
--- /dev/null
+++ b/modules/pfr/plotsr/meta.yml
@@ -0,0 +1,79 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "plotsr"
+description: Plotsr generates high-quality visualisation of synteny and structural rearrangements between multiple genomes.
+keywords:
+  - genomics
+  - synteny
+  - rearrangements
+  - chromosome
+tools:
+  - "plotsr":
+      description: "Visualiser for structural annotations between multiple genomes"
+      homepage: "https://github.com/schneebergerlab/plotsr"
+      documentation: "https://github.com/schneebergerlab/plotsr"
+      tool_dev_url: "https://github.com/schneebergerlab/plotsr"
+      doi: "10.1093/bioinformatics/btac196"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - syri:
+      type: file
+      description: Structural annotation mappings (syri.out) identified by SyRI
+      pattern: "*syri.out"
+  - fastas:
+      type: list
+      description: Fasta files in the sequence specified by the `genomes` file
+      pattern: "*.{fasta,fa,fsa,faa}"
+  - genomes:
+      type: string
+      description: |
+        String containing the genomes.txt file contents including the header, but excluding the path to genome fasta files.
+        The path to staged genome files is automatically added by the process script. For example, see the included nf-test.
+      pattern: "*.txt"
+  - bedpe:
+      type: file
+      description: Structural annotation mappings in BEDPE format
+      pattern: "*.bedpe"
+  - markers:
+      type: file
+      description: File containing path to markers
+      pattern: "*.bed"
+  - tracks:
+      type: file
+      description: File listing paths and details for all tracks to be plotted
+      pattern: "*.txt"
+  - chrord:
+      type: file
+      description: |
+        File containing reference (first genome) chromosome IDs in the order in which they are to be plotted.
+        File requires one chromosome ID per line. Not compatible with --chr
+      pattern: "*.txt"
+  - chrname:
+      type: file
+      description: |
+        File containing reference (first genome) chromosome names to be used in the plot.
+        File needs to be a TSV with the chromosome ID in first column and chromosome name in the second.
+      pattern: "*.txt"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - png:
+      type: file
+      description: Synteny plot
+      pattern: "*.png"
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/modules/pfr/plotsr/tests/main.nf.test b/modules/pfr/plotsr/tests/main.nf.test
new file mode 100644
index 00000000..f02b8ebf
--- /dev/null
+++ b/modules/pfr/plotsr/tests/main.nf.test
@@ -0,0 +1,121 @@
+nextflow_process {
+
+    name "Test Process PLOTSR"
+    script "../main.nf"
+    config './nextflow.config'
+    process "PLOTSR"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "plotsr"
+    tag "minimap2/align"
+    tag "syri"
+
+    setup {
+        run("MINIMAP2_ALIGN") {
+            script "modules/pfr/minimap2/align/main.nf"
+            process {
+                """
+                def genome2_cleanid     = file('genome2_cleanid.fasta')
+                genome2_cleanid.text    = file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true).text.replace(/>chr22:16600000-16800000/, '>chr22')
+
+                input[0] = [
+                    [id: 'test'],
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = [
+                    [id: 'reference'],
+                    genome2_cleanid
+                ]
+                input[2] = true     // bam_format
+                input[3] = false    // cigar_paf_format
+                input[4] = false    // cigar_bam
+                """
+            }
+        }
+
+        run("SYRI") {
+            script "modules/pfr/syri/main.nf"
+            process {
+                """
+                def genome2_cleanid     = file('genome2_cleanid.fasta')
+                genome2_cleanid.text    = file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true).text.replace(/>chr22:16600000-16800000/, '>chr22')
+
+                input[0] = MINIMAP2_ALIGN.out.bam
+                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                input[2] = genome2_cleanid
+                input[3] = 'B'
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens - genome") {
+
+        when {
+            process {
+                """
+                def genome2_cleanid     = file('genome2_cleanid.fasta')
+
+                input[0] = SYRI.out.syri
+                input[1] = [
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
+                    genome2_cleanid
+                ]
+
+                input[2] = "#file	name	tags\\nquery   lw:1.5\\nreference  lw:1.5\\n"
+                input[3] = []
+                input[4] = []
+                input[5] = []
+                input[6] = []
+                input[7] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.versions).match() },
+                { assert file(process.out.png[0][1]).name }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - genome - stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                def genome2_cleanid     = file('genome2_cleanid.fasta')
+
+                input[0] = SYRI.out.syri
+                input[1] = [
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
+                    genome2_cleanid
+                ]
+
+                input[2] = "#file	name	tags\\nquery   lw:1.5\\nreference  lw:1.5\\n"
+                input[3] = []
+                input[4] = []
+                input[5] = []
+                input[6] = []
+                input[7] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.versions).match() },
+                { assert file(process.out.png[0][1]).name }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/pfr/plotsr/tests/main.nf.test.snap b/modules/pfr/plotsr/tests/main.nf.test.snap
new file mode 100644
index 00000000..5488c5c8
--- /dev/null
+++ b/modules/pfr/plotsr/tests/main.nf.test.snap
@@ -0,0 +1,26 @@
+{
+    "homo_sapiens - genome": {
+        "content": [
+            [
+                "versions.yml:md5,73ec2873447f67f41edb762294f8f7c5"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-23T17:20:57.183473"
+    },
+    "homo_sapiens - genome - stub": {
+        "content": [
+            [
+                "versions.yml:md5,73ec2873447f67f41edb762294f8f7c5"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-23T20:28:10.557465"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/plotsr/tests/nextflow.config b/modules/pfr/plotsr/tests/nextflow.config
new file mode 100644
index 00000000..959099ae
--- /dev/null
+++ b/modules/pfr/plotsr/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: MINIMAP2_ALIGN {
+        ext.args = '-x asm5 --eqx'
+    }
+}
diff --git a/modules/pfr/plotsr/tests/tags.yml b/modules/pfr/plotsr/tests/tags.yml
new file mode 100644
index 00000000..0a8616fd
--- /dev/null
+++ b/modules/pfr/plotsr/tests/tags.yml
@@ -0,0 +1,2 @@
+plotsr:
+  - "modules/pfr/plotsr/**"
diff --git a/modules/pfr/syri/environment.yml b/modules/pfr/syri/environment.yml
new file mode 100644
index 00000000..45eafb1f
--- /dev/null
+++ b/modules/pfr/syri/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "syri"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - "bioconda::syri=1.6.3"
diff --git a/modules/pfr/syri/main.nf b/modules/pfr/syri/main.nf
new file mode 100644
index 00000000..0a10ae19
--- /dev/null
+++ b/modules/pfr/syri/main.nf
@@ -0,0 +1,60 @@
+process SYRI {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/syri:1.6.3--py38hdbdd923_2':
+        'biocontainers/syri:1.6.3--py38hdbdd923_2' }"
+
+    input:
+    tuple val(meta), path(infile)
+    path(query_fasta)
+    path(reference_fasta)
+    val(file_type)
+
+    output:
+    tuple val(meta), path("*syri.out")      , emit: syri        , optional: true
+    tuple val(meta), path("*.error.log")    , emit: error       , optional: true
+    path "versions.yml"                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if ( ! ( file_type in [ 'T', 'S', 'B', 'P' ] ) ) { error "File type should be one of [ 'T', 'S', 'B', 'P' ]" }
+    """
+    syri \\
+        -c $infile \\
+        -q $query_fasta \\
+        -r $reference_fasta \\
+        -F $file_type \\
+        $args \\
+        --prefix $prefix \\
+        2> >(tee "${prefix}.error.log" >&2) \\
+        || echo "Errors from syri printed to ${prefix}.error.log"
+
+    [ -f "${prefix}syri.out" ] \\
+        && rm "${prefix}.error.log" \\
+        || echo 'Syri failed and no syri.out file was created'
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        syri: \$(syri --version)
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}syri.out
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        syri: \$(syri --version)
+    END_VERSIONS
+    """
+}
diff --git a/modules/pfr/syri/meta.yml b/modules/pfr/syri/meta.yml
new file mode 100644
index 00000000..d7801532
--- /dev/null
+++ b/modules/pfr/syri/meta.yml
@@ -0,0 +1,64 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "syri"
+description: Syri compares alignments between two chromosome-level assemblies and identifies synteny and structural rearrangements.
+keywords:
+  - genomics
+  - synteny
+  - rearrangements
+  - chromosome
+tools:
+  - "syri":
+      description: "Synteny and rearrangement identifier between whole-genome assemblies"
+      homepage: "https://github.com/schneebergerlab/syri"
+      documentation: "https://github.com/schneebergerlab/syri"
+      tool_dev_url: "https://github.com/schneebergerlab/syri"
+      doi: "10.1186/s13059-019-1911-0"
+      licence: ["MIT License"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - infile:
+      type: file
+      description: File containing alignment coordinates
+      pattern: "*.{table, sam, bam, paf}"
+  - query_fasta:
+      type: file
+      description: Query genome for the alignments
+      pattern: "*.fasta"
+  - reference_fasta:
+      type: file
+      description: Reference genome for the alignments
+      pattern: "*.fasta"
+  - file_type:
+      type: string
+      description: |
+        Input file type which is one of  T: Table, S: SAM, B: BAM, P: PAF
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - syri:
+      type: file
+      description: Syri output file
+      pattern: "*syri.out"
+  - error:
+      type: file
+      description: |
+        Error log if syri fails. This error log enables the pipeline to detect if syri has failed due to one of its
+        known limitations and pass the information to the user in a user-friendly manner such as a HTML report
+      pattern: "*.error.log"
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/modules/pfr/syri/tests/main.nf.test b/modules/pfr/syri/tests/main.nf.test
new file mode 100644
index 00000000..baa8555c
--- /dev/null
+++ b/modules/pfr/syri/tests/main.nf.test
@@ -0,0 +1,80 @@
+nextflow_process {
+
+    name "Test Process SYRI"
+    script "../main.nf"
+    config './nextflow.config'
+    process "SYRI"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "syri"
+    tag "minimap2/align"
+
+    setup {
+        run("MINIMAP2_ALIGN") {
+            script "modules/pfr/minimap2/align/main.nf"
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[1] = [
+                    [id: 'reference'],
+                    file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
+                ]
+                input[2] = true     // bam_format
+                input[3] = false    // cigar_paf_format
+                input[4] = false    // cigar_bam
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens - genome") {
+
+        when {
+            process {
+                """
+                input[0] = MINIMAP2_ALIGN.out.bam
+                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                input[2] = file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
+                input[3] = 'B'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - genome - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = MINIMAP2_ALIGN.out.bam
+                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                input[2] = file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true)
+                input[3] = 'B'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/pfr/syri/tests/main.nf.test.snap b/modules/pfr/syri/tests/main.nf.test.snap
new file mode 100644
index 00000000..8d09bf33
--- /dev/null
+++ b/modules/pfr/syri/tests/main.nf.test.snap
@@ -0,0 +1,80 @@
+{
+    "homo_sapiens - genome": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "testsyri.out:md5,fd2a1b676912818960056aa281177bb4"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,05cf55e24c29c2661a2b9301d3c7c2a0"
+                ],
+                "error": [
+                    
+                ],
+                "syri": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "testsyri.out:md5,fd2a1b676912818960056aa281177bb4"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,05cf55e24c29c2661a2b9301d3c7c2a0"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-25T21:30:50.653501"
+    },
+    "homo_sapiens - genome - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "testsyri.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,05cf55e24c29c2661a2b9301d3c7c2a0"
+                ],
+                "error": [
+                    
+                ],
+                "syri": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "testsyri.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,05cf55e24c29c2661a2b9301d3c7c2a0"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-25T21:22:31.028411"
+    }
+}
\ No newline at end of file
diff --git a/modules/pfr/syri/tests/nextflow.config b/modules/pfr/syri/tests/nextflow.config
new file mode 100644
index 00000000..959099ae
--- /dev/null
+++ b/modules/pfr/syri/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: MINIMAP2_ALIGN {
+        ext.args = '-x asm5 --eqx'
+    }
+}
diff --git a/modules/pfr/syri/tests/tags.yml b/modules/pfr/syri/tests/tags.yml
new file mode 100644
index 00000000..3afda524
--- /dev/null
+++ b/modules/pfr/syri/tests/tags.yml
@@ -0,0 +1,2 @@
+syri:
+  - "modules/pfr/syri/**"
diff --git a/nextflow.config b/nextflow.config
index 9203b2a6..23837203 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -52,14 +52,18 @@ params {
 
     // Synteny options
     synteny_skip                        = true
-    synteny_plot_type                   = 'both'
+    synteny_mummer_skip                 = true
+    synteny_plotsr_skip                 = true
+    synteny_xref_assemblies             = null
     synteny_between_input_assemblies    = true
-    synteny_many_to_many_align          = false
-    synteny_max_gap                     = 1000000
-    synteny_min_bundle_size             = 1000
+    synteny_mummer_plot_type            = 'both'
+    synteny_mummer_m2m_align            = false
+    synteny_mummer_max_gap              = 1000000
+    synteny_mummer_min_bundle_size      = 1000
     synteny_plot_1_vs_all               = false
     synteny_color_by_contig             = true
-    synteny_xref_assemblies             = null
+    synteny_plotsr_seq_label            = 'Chr'
+    synteny_plotsr_assembly_order       = null
 
     // Merqury options
     merqury_skip                        = true
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 44d71353..f6b73930 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -46,7 +46,8 @@
                 "assemblathon_stats_n_limit": {
                     "type": "integer",
                     "default": 100,
-                    "description": "The number of 'N's for the unknown gap size. NCBI recommendation is 100"
+                    "description": "The number of 'N's for the unknown gap size. NCBI recommendation is 100",
+                    "fa_icon": "fas fa-ruler-horizontal"
                 }
             }
         },
@@ -65,7 +66,8 @@
                 "ncbi_fcs_adaptor_empire": {
                     "type": "string",
                     "enum": ["euk", "prok"],
-                    "description": "Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes"
+                    "description": "Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes",
+                    "fa_icon": "fas fa-project-diagram"
                 },
                 "ncbi_fcs_gx_skip": {
                     "type": "boolean",
@@ -75,7 +77,8 @@
                 },
                 "ncbi_fcs_gx_tax_id": {
                     "type": "number",
-                    "description": "Tax ID for NCBI FCS GX. See: https://www.ncbi.nlm.nih.gov/taxonomy"
+                    "description": "Tax ID for NCBI FCS GX. See: https://www.ncbi.nlm.nih.gov/taxonomy",
+                    "fa_icon": "fab fa-orcid"
                 },
                 "ncbi_fcs_gx_db_path": {
                     "type": "string",
@@ -106,12 +109,14 @@
                 "busco_mode": {
                     "type": "string",
                     "enum": ["genome", "transcriptome", "proteins"],
-                    "description": "BUSCO mode: 'genome', 'transcriptome', 'proteins'"
+                    "description": "BUSCO mode: 'genome', 'transcriptome', 'proteins'",
+                    "fa_icon": "fas fa-cog"
                 },
                 "busco_lineage_datasets": {
                     "type": "string",
                     "pattern": "^(\\w+_odb10\\s)*\\w+_odb10$",
-                    "description": "BUSCO lineages. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10'"
+                    "description": "BUSCO lineages. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10'",
+                    "fa_icon": "fas fa-clipboard-list"
                 },
                 "busco_download_path": {
                     "type": "string",
@@ -135,17 +140,20 @@
                 },
                 "tidk_repeat_seq": {
                     "type": "string",
-                    "description": "Telomere repeat sequence. Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG",
-                    "pattern": "^[ACGT]+$"
+                    "description": "Telomere repeat sequence. Typical values for plant: TTTAGGG, fungus, vertebrates: TTAGGG and Insect: TTAGG",
+                    "pattern": "^[ACGT]+$",
+                    "fa_icon": "fas fa-dna"
                 },
                 "tidk_filter_by_size": {
                     "type": "boolean",
-                    "description": "Filter size in base-pairs"
+                    "description": "Filter assembly sequences smaller than the specified length",
+                    "fa_icon": "fas fa-question-circle"
                 },
                 "tidk_filter_size_bp": {
                     "type": "integer",
                     "default": 1000000,
-                    "description": "Filter size in base-pairs"
+                    "description": "Filter size in base-pairs",
+                    "fa_icon": "fas fa-ruler-horizontal"
                 }
             }
         },
@@ -207,7 +215,8 @@
                 "hic_fastp_ext_args": {
                     "type": "string",
                     "default": "--qualified_quality_phred 20 --length_required 50",
-                    "description": "Additional parameters for fastp trimming"
+                    "description": "Additional parameters for fastp trimming",
+                    "fa_icon": "fas fa-terminal"
                 }
             }
         },
@@ -223,46 +232,78 @@
                     "description": "Skip synteny analysis",
                     "fa_icon": "fas fa-forward"
                 },
-                "synteny_plot_type": {
+                "synteny_mummer_skip": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Skip Mummer-based synteny analysis",
+                    "fa_icon": "fas fa-forward"
+                },
+                "synteny_plotsr_skip": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Skip plotsr-based synteny analysis",
+                    "fa_icon": "fas fa-forward"
+                },
+                "synteny_xref_assemblies": {
                     "type": "string",
-                    "default": "both",
-                    "description": "Synteny plot type: 'linear', 'circos', or 'both'",
-                    "enum": ["both", "linear", "circos"]
+                    "description": "Reference assemblies for synteny analysis",
+                    "format": "file-path",
+                    "mimetype": "csv",
+                    "schema": "assets/schema_xref_assemblies.json",
+                    "fa_icon": "fas fa-file-csv"
                 },
                 "synteny_between_input_assemblies": {
                     "type": "boolean",
                     "description": "Create syntenic plots between each pair of input assemblies",
-                    "default": true
+                    "default": true,
+                    "fa_icon": "fas fa-question-circle"
+                },
+                "synteny_mummer_plot_type": {
+                    "type": "string",
+                    "default": "both",
+                    "description": "Synteny plot type from Mummer alignments: 'dotplot', 'circos', or 'both'",
+                    "enum": ["both", "dotplot", "circos"],
+                    "fa_icon": "fas fa-chart-line"
                 },
-                "synteny_many_to_many_align": {
+                "synteny_mummer_m2m_align": {
                     "type": "boolean",
-                    "description": "Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)"
+                    "description": "Include Mummer alignment blocks with many-to-many mappings",
+                    "fa_icon": "fas fa-question-circle"
                 },
-                "synteny_max_gap": {
+                "synteny_mummer_max_gap": {
                     "type": "integer",
                     "default": 1000000,
-                    "description": "Alignments within this distance are bundled together"
+                    "description": "Mummer alignments within this distance are bundled together",
+                    "fa_icon": "fas fa-ruler-horizontal"
                 },
-                "synteny_min_bundle_size": {
+                "synteny_mummer_min_bundle_size": {
                     "type": "integer",
                     "default": 1000,
-                    "description": "After bundling, any bundle smaller than this size is filtered out"
+                    "description": "After bundling, any Mummer alignment bundle smaller than this size is filtered out",
+                    "fa_icon": "fas fa-ruler-horizontal"
                 },
                 "synteny_plot_1_vs_all": {
                     "type": "boolean",
-                    "description": "Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly"
+                    "description": "Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly. This only applies to Mummer plots",
+                    "fa_icon": "fas fa-question-circle"
                 },
                 "synteny_color_by_contig": {
                     "type": "boolean",
                     "default": true,
-                    "description": "Synteny plot is colored by contig"
+                    "description": "Mummer synteny plots are colored by contig. Otherwise, they are colored by bundle size",
+                    "fa_icon": "fas fa-question-circle"
                 },
-                "synteny_xref_assemblies": {
+                "synteny_plotsr_seq_label": {
                     "type": "string",
-                    "description": "Reference assemblies for synteny analysis",
-                    "format": "file-path",
-                    "mimetype": "csv",
-                    "schema": "assets/schema_xref_assemblies.json"
+                    "default": "Chr",
+                    "description": "Sequence label prefix for plotsr synteny",
+                    "fa_icon": "fas fa-tag"
+                },
+                "synteny_plotsr_assembly_order": {
+                    "type": "string",
+                    "fa_icon": "fas fa-sort-alpha-down",
+                    "pattern": "^(\\w+\\s)*\\w+$",
+                    "description": "The order in which the assemblies should be compared, provided as space separated string of assembly tags. If absent, assemblies are ordered by their tags alphabetically."
                 }
             }
         },
@@ -282,7 +323,8 @@
                     "type": "integer",
                     "default": 21,
                     "description": "kmer length for merqury analysis",
-                    "minimum": 3
+                    "minimum": 3,
+                    "fa_icon": "fas fa-ruler-horizontal"
                 }
             }
         },
diff --git a/pfr/params.json b/pfr/params.json
index d6f32ff5..1b7715be 100644
--- a/pfr/params.json
+++ b/pfr/params.json
@@ -23,11 +23,11 @@
     "hic_skip_fastqc": false,
     "hic_fastp_ext_args": "--qualified_quality_phred 20 --length_required 50",
     "synteny_skip": false,
-    "synteny_plot_type": "both",
+    "synteny_mummer_plot_type": "both",
     "synteny_between_input_assemblies": true,
-    "synteny_many_to_many_align": false,
-    "synteny_max_gap": 1000000,
-    "synteny_min_bundle_size": 1000,
+    "synteny_mummer_m2m_align": false,
+    "synteny_mummer_max_gap": 1000000,
+    "synteny_mummer_min_bundle_size": 1000,
     "synteny_plot_1_vs_all": false,
     "synteny_color_by_contig": true,
     "synteny_xref_assemblies": "/workspace/assemblyqc/testdata/default/xrefsheet.csv",
diff --git a/subworkflows/local/fasta_synteny.nf b/subworkflows/local/fasta_synteny.nf
index 26d31a0d..9f8afaa0 100644
--- a/subworkflows/local/fasta_synteny.nf
+++ b/subworkflows/local/fasta_synteny.nf
@@ -11,6 +11,10 @@ include { RELABELFASTALENGTH            } from '../../modules/local/relabelfasta
 include { GENERATEKARYOTYPE             } from '../../modules/local/generatekaryotype'
 include { CIRCOS                        } from '../../modules/local/circos'
 include { LINEARSYNTENY                 } from '../../modules/local/linearsynteny'
+include { CUSTOM_RELABELFASTA           } from '../../modules/pfr/custom/relabelfasta/main'
+include { MINIMAP2_ALIGN                } from '../../modules/nf-core/minimap2/align/main'
+include { SYRI                          } from '../../modules/pfr/syri/main'
+include { PLOTSR                        } from '../../modules/pfr/plotsr/main'
 
 workflow FASTA_SYNTENY {
     take:
@@ -18,12 +22,16 @@ workflow FASTA_SYNTENY {
     ch_labels                           // Channel: [ tag, txt ]
     ch_xref_fasta_labels                // Channel: [ tag2, fa, txt ]
     between_input_assemblies            // val(true|false)
-    many_to_many_align                  // val(true|false)
-    max_gap                             // val(Integer)
-    min_bundle_size                     // val(Integer)
+    mummer_m2m_align                    // val(true|false)
+    mummer_max_gap                      // val(Integer)
+    mummer_min_bundle_size              // val(Integer)
     plot_1_vs_all                       // val(true|false)
     color_by_contig                     // val(true|false)
-    plot_type                           // val(linear|circos|both)
+    mummer_plot_type                    // val(linear|circos|both)
+    mummer_skip                         // val(true|false)
+    plotsr_seq_label                    // val(String)
+    plotsr_skip                         // val(true|false)
+    plotsr_assembly_order               // val(String)
 
     main:
     ch_versions                         = Channel.empty()
@@ -33,7 +41,7 @@ workflow FASTA_SYNTENY {
                                             ch_labels
                                         )
 
-    ch_input_combinations               = ! between_input_assemblies
+    ch_input_combination                = ! between_input_assemblies
                                         ? Channel.empty()
                                         : ch_fasta_labels
                                         | map { [it] }
@@ -54,11 +62,13 @@ workflow FASTA_SYNTENY {
     // MODULE: GUNZIP_FASTA
     GUNZIP_FASTA ( ch_xref_fa_branch.gz )
 
-    ch_xref_ungz_fa_labels              = GUNZIP_FASTA.out.gunzip
+    ch_xref_ungz                        = GUNZIP_FASTA.out.gunzip
                                         | mix(
                                             ch_xref_fa_branch.rest
                                         )
                                         | map { meta, fa -> [ meta.id, fa ] }
+
+    ch_xref_ungz_fa_labels              = ch_xref_ungz
                                         | join(
                                             ch_xref_fasta_labels
                                         )
@@ -66,7 +76,9 @@ workflow FASTA_SYNTENY {
                                             [ tag, fa, seq_list ]
                                         }
 
-    ch_all_combinations                 = ch_input_combinations
+    ch_combination                      = mummer_skip
+                                        ? Channel.empty()
+                                        : ch_input_combination
                                         | mix(
                                             ch_fasta_labels
                                             | combine(
@@ -74,7 +86,7 @@ workflow FASTA_SYNTENY {
                                             )
                                         )
 
-    ch_all_combination_labels           = ch_all_combinations
+    ch_combination_labels               = ch_combination
                                         | map { target_tag, target_fa, target_txt, xref_tag, xref_fa, xref_txt ->
                                             [ "${target_tag}.on.${xref_tag}", target_txt, xref_txt ]
                                         }
@@ -82,7 +94,7 @@ workflow FASTA_SYNTENY {
     ch_versions                         = ch_versions.mix(GUNZIP_FASTA.out.versions.first())
 
     // MODULE: FILTERSORTFASTA
-    FILTERSORTFASTA ( ch_all_combinations )
+    FILTERSORTFASTA ( ch_combination )
 
     ch_versions                         = ch_versions.mix(FILTERSORTFASTA.out.versions.first())
 
@@ -106,7 +118,7 @@ workflow FASTA_SYNTENY {
                                         )
     DNADIFF(
         ch_dnadiff_inputs,
-        many_to_many_align
+        mummer_m2m_align
     )
 
     ch_versions                         = ch_versions.mix(DNADIFF.out.versions.first())
@@ -114,8 +126,8 @@ workflow FASTA_SYNTENY {
     // MODULE: BUNDLELINKS
     BUNDLELINKS(
         DNADIFF.out.coords,
-        max_gap,
-        min_bundle_size
+        mummer_max_gap,
+        mummer_min_bundle_size
     )
 
     ch_versions                         = ch_versions.mix(BUNDLELINKS.out.versions.first())
@@ -131,7 +143,7 @@ workflow FASTA_SYNTENY {
 
     // MODULE: RELABELBUNDLELINKS
     ch_relabellinks_inputs              = ch_coloured_links
-                                        | join(ch_all_combination_labels)
+                                        | join(ch_combination_labels)
 
     RELABELBUNDLELINKS ( ch_relabellinks_inputs )
 
@@ -152,7 +164,7 @@ workflow FASTA_SYNTENY {
 
     // MODULE: RELABELFASTALENGTH
     ch_relabelfastalength_inputs        = GETFASTALENGTH.out.length
-                                        | join(ch_all_combination_labels)
+                                        | join(ch_combination_labels)
 
     RELABELFASTALENGTH ( ch_relabelfastalength_inputs )
 
@@ -178,7 +190,7 @@ workflow FASTA_SYNTENY {
     ch_versions                         = ch_versions.mix(GENERATEKARYOTYPE.out.versions.first())
 
     // MODULE: CIRCOS
-    ch_circos_inputs                    = ( plot_type in [ 'circos', 'both' ] )
+    ch_circos_inputs                    = ( mummer_plot_type in [ 'circos', 'both' ] )
                                         ? ch_split_links
                                         | map { target_on_xref, seq_tag, txt ->
                                             [ "${target_on_xref}.${seq_tag}", txt ]
@@ -190,7 +202,7 @@ workflow FASTA_SYNTENY {
     ch_versions                         = ch_versions.mix(CIRCOS.out.versions.first())
 
     // MODULE: LINEARSYNTENY
-    ch_linear_synteny_inputs            = ( plot_type in [ 'linear', 'both' ] )
+    ch_linear_synteny_inputs            = ( mummer_plot_type in [ 'dotplot', 'both' ] )
                                         ? ch_split_links
                                         | map { target_on_xref, seq_tag, txt ->
                                             [ "${target_on_xref}.${seq_tag}", txt ]
@@ -203,9 +215,164 @@ workflow FASTA_SYNTENY {
 
     ch_versions                         = ch_versions.mix(LINEARSYNTENY.out.versions.first())
 
+    // Create chr label lists
+    ch_assembly_labels                  = plotsr_skip
+                                        ? Channel.empty()
+                                        : ch_fasta_labels
+                                        | mix(ch_xref_ungz_fa_labels)
+
+    ch_common_label_count               = ch_assembly_labels
+                                        | map { tag, fa, labels ->
+                                            labels.readLines().findAll { it != '' }.size()
+                                        }
+                                        | collect
+                                        | map { it.min() }
+
+    ch_plotsr_formatted_labels          = ch_assembly_labels
+                                        | combine(ch_common_label_count)
+                                        | map { tag, fa, labels, num ->
+                                            def label_lines = labels
+                                                .readLines()
+                                                .collect { it.trim() }
+                                                .findAll { it != '' }
+
+                                            label_lines.each { line ->
+                                                if ( line.split('\t').size() != 2 ) {
+                                                    error "synteny_labels file ${labels.name} for assembly ${tag} is malformed near line ${line}"
+                                                }
+                                            }
+
+                                            def new_labels = label_lines[0..<num].withIndex().collect { line, index ->
+                                                def literals = line.split('\t')
+                                                def seq = literals[0]
+                                                def label = "${plotsr_seq_label}${index+1}"
+
+                                                "$seq\t$label"
+                                            }.join('\n')
+
+                                            [ "${tag}.plotsr.csv", new_labels]
+                                        }
+                                        | collectFile(storeDir: "${params.outdir}/synteny/plotsr")
+                                        | map { labels -> [ labels.baseName.replace('.plotsr', ''), labels ] }
+
+    // MODULE: CUSTOM_RELABELFASTA
+    ch_relabel_inputs                   = ch_assembly_labels
+                                        | join(ch_plotsr_formatted_labels)
+                                        | map { tag, fa, old_l, new_l -> [ tag, fa, new_l ] }
+    CUSTOM_RELABELFASTA(
+        ch_relabel_inputs.map { tag, fa, labels -> [ [ id: tag ], fa ] },
+        ch_relabel_inputs.map { tag, fa, labels -> labels }
+    )
+
+    ch_plotsr_assembly                  = CUSTOM_RELABELFASTA.out.fasta
+    ch_versions                         = ch_versions.mix(CUSTOM_RELABELFASTA.out.versions.first())
+
+    // MODULE: MINIMAP2_ALIGN
+    ch_minimap_inputs                   = ch_plotsr_assembly
+                                        | map { [ it ] }
+                                        | collect
+                                        | map { list ->
+                                            if ( plotsr_assembly_order == null) {
+                                                return list.sort(false) { it[0].id.toUpperCase() }
+                                            }
+
+                                            def order   = plotsr_assembly_order.tokenize(' ')
+                                            def tags    = list.collect { it[0].id }
+
+                                            def ordered_list = []
+                                            order.each { tag ->
+                                                if ( ! ( tag in tags ) ) error "Assembly $tag listed in synteny_plotsr_assembly_order could not be found in input or synteny_xref_assemblies: $tags"
+
+                                                ordered_list << ( list.find { it[0].id == tag } )
+                                            }
+                                            ordered_list
+                                        }
+                                        | flatMap { list ->
+                                            if ( list.size() < 2 ) return null
+
+                                            def new_list = []
+                                            list.eachWithIndex { assembly, index -> if ( index > 0 ) { new_list << [ assembly, list[index-1] ] } }
+                                            new_list
+                                        }
+                                        | flatten
+                                        | buffer(size: 4)
+                                        | map { tmeta, tfa, rmeta, rfa ->
+                                            [ [ id: "${tmeta.id}.on.${rmeta.id}" ], tfa, rfa ]
+                                        }
+    MINIMAP2_ALIGN(
+        ch_minimap_inputs.map { meta, tfa, rfa -> [ meta, tfa ] },
+        ch_minimap_inputs.map { meta, tfa, rfa -> [ meta, rfa ] },
+        true,   // bam_format
+        false,  // cigar_paf_format
+        false   // cigar_bam
+    )
+
+    ch_minimap2_bam                     = MINIMAP2_ALIGN.out.bam
+    ch_versions                         = ch_versions.mix(MINIMAP2_ALIGN.out.versions.first())
+
+    // MODULE: SYRI
+    ch_syri_inputs                      = ch_minimap2_bam
+                                        | join(ch_minimap_inputs)
+
+    SYRI(
+        ch_syri_inputs.map { meta, bam, tfa, rfa -> [ meta, bam ] },
+        ch_syri_inputs.map { meta, bam, tfa, rfa -> tfa },
+        ch_syri_inputs.map { meta, bam, tfa, rfa -> rfa },
+        'B' // BAM
+    )
+
+    ch_syri                             = SYRI.out.syri
+    ch_syri_fail_log                    = SYRI.out.error
+    ch_versions                         = ch_versions.mix(SYRI.out.versions.first())
+
+    // MODULE: PLOTSR
+    ch_plotsr_inputs                    = ch_syri
+                                        | join(ch_syri_inputs)
+                                        | map { meta, syri, bam, tfa, rfa ->
+
+                                            [
+                                                [ id: 'plotsr' ],
+                                                syri,
+                                                [ rfa, tfa ] // Order matters, see https://github.com/schneebergerlab/plotsr/issues/70
+                                            ]
+                                        }
+                                        | groupTuple
+                                        | map { meta, syri, fastas ->
+
+                                            def unique_fa = []
+
+                                            fastas.flatten().each { fasta ->
+                                                if ( ! ( fasta in unique_fa ) ) { unique_fa << fasta }
+                                            }
+
+                                            [
+                                                meta,
+                                                syri,
+                                                unique_fa,
+                                                "#file\tname\n" + unique_fa.collect { it.baseName.replace('.plotsr', '') }.join('\n')
+                                            ]
+                                        }
+
+    PLOTSR(
+        ch_plotsr_inputs.map { meta, syri, fastas, txt -> [ meta, syri ] },
+        ch_plotsr_inputs.map { meta, syri, fastas, txt -> fastas },
+        ch_plotsr_inputs.map { meta, syri, fastas, txt -> txt },
+        [],
+        [],
+        [],
+        [],
+        []
+    )
+
+    ch_plotsr_png                       = PLOTSR.out.png
+    ch_versions                         = ch_versions.mix(PLOTSR.out.versions.first())
+
     emit:
     png                                 = CIRCOS.out.png_file
+                                        | mix( ch_plotsr_png.map { meta, png -> png } )
     html                                = LINEARSYNTENY.out.html
+    syri_fail_log                       = ch_syri_fail_log.map { meta, log -> log }
+    plotsr_labels                       = ch_plotsr_formatted_labels.map { tag, labels -> labels }
     versions                            = ch_versions
 }
 
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index e26f3466..282e5c6d 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -533,15 +533,22 @@ workflow ASSEMBLYQC {
         ch_synteny_labels.map { meta, txt -> [ meta.id, txt ] },
         ch_xref_assembly,
         params.synteny_between_input_assemblies,
-        params.synteny_many_to_many_align,
-        params.synteny_max_gap,
-        params.synteny_min_bundle_size,
+        params.synteny_mummer_m2m_align,
+        params.synteny_mummer_max_gap,
+        params.synteny_mummer_min_bundle_size,
         params.synteny_plot_1_vs_all,
         params.synteny_color_by_contig,
-        params.synteny_plot_type
+        params.synteny_mummer_plot_type,
+        params.synteny_mummer_skip,
+        params.synteny_plotsr_seq_label,
+        params.synteny_plotsr_skip,
+        params.synteny_plotsr_assembly_order
     )
 
-    ch_synteny_plots                        = FASTA_SYNTENY.out.png.mix(FASTA_SYNTENY.out.html)
+    ch_synteny_outputs                      = FASTA_SYNTENY.out.png
+                                            | mix(FASTA_SYNTENY.out.html)
+                                            | mix(FASTA_SYNTENY.out.syri_fail_log)
+                                            | mix(FASTA_SYNTENY.out.plotsr_labels)
     ch_versions                             = ch_versions.mix(FASTA_SYNTENY.out.versions)
 
     // MODULE: MERYL_COUNT
@@ -731,7 +738,8 @@ workflow ASSEMBLYQC {
                                                 storeDir: "${params.outdir}/pipeline_info",
                                                 name: 'software_versions.yml',
                                                 sort: true,
-                                                newLine: true
+                                                newLine: true,
+                                                cache: false
                                             )
 
     // MODULE: CREATEREPORT
@@ -748,7 +756,7 @@ workflow ASSEMBLYQC {
         ch_lai_outputs                      .collect().ifEmpty([]),
         ch_kraken2_plot                     .collect().ifEmpty([]),
         ch_hic_html                         .collect().ifEmpty([]),
-        ch_synteny_plots                    .collect().ifEmpty([]),
+        ch_synteny_outputs                  .collect().ifEmpty([]),
         ch_merqury_outputs                  .collect().ifEmpty([]),
         ch_versions_yml,
         ch_params_as_json,

From 5d3fe264f52774ec756b61ecd4dde5a5e41bf6d8 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 27 May 2024 17:31:38 +1200
Subject: [PATCH 52/76] Upgraded locally merqury

---
 CHANGELOG.md                                  |   7 +-
 conf/modules.config                           |   2 +-
 modules.json                                  |   7 +-
 modules/nf-core/merqury/merqury.diff          |  44 --
 .../merqury/{ => merqury}/environment.yml     |   2 +-
 modules/nf-core/merqury/{ => merqury}/main.nf |   7 +-
 .../nf-core/merqury/{ => merqury}/meta.yml    |   9 +-
 .../merqury/merqury/tests/main.nf.test        | 189 +++++++
 .../merqury/merqury/tests/main.nf.test.snap   | 494 ++++++++++++++++++
 .../nf-core/merqury/merqury/tests/tags.yml    |   2 +
 workflows/assemblyqc.nf                       |  16 +-
 11 files changed, 712 insertions(+), 67 deletions(-)
 delete mode 100644 modules/nf-core/merqury/merqury.diff
 rename modules/nf-core/merqury/{ => merqury}/environment.yml (80%)
 rename modules/nf-core/merqury/{ => merqury}/main.nf (88%)
 rename modules/nf-core/merqury/{ => merqury}/meta.yml (94%)
 create mode 100644 modules/nf-core/merqury/merqury/tests/main.nf.test
 create mode 100644 modules/nf-core/merqury/merqury/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/merqury/merqury/tests/tags.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9179f33a..724eb313 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0+dev - [22-May-2024]
+## v2.0.0+dev - [27-May-2024]
 
 ### `Added`
 
@@ -21,9 +21,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 12. Replaced `GFF3_VALIDATE` sub-workflow with `GFF3_GT_GFF3_GFF3VALIDATOR_STAT`
 13. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
 14. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
-15. Locally patched `MERQURY`
-16. Added PLOTSR [#77](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/77)
-17. Added [JADWOS01](https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_016859245.1/) assembly to xrefsheet for successfully running PLOTSR.
+15. Added PLOTSR [#77](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/77)
+16. Added [JADWOS01](https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_016859245.1/) assembly to xrefsheet for successfully running PLOTSR.
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index d199e968..5082199d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -350,7 +350,7 @@ process {
         ext.prefix = { "${meta.id}.fasta" }
     }
 
-    withName: MERQURY {
+    withName: MERQURY_MERQURY {
         publishDir = [
             [
                 path: { "${params.outdir}/merqury/${meta.id}" },
diff --git a/modules.json b/modules.json
index 2e69aedd..2f81369d 100644
--- a/modules.json
+++ b/modules.json
@@ -170,11 +170,10 @@
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
                         "installed_by": ["modules"]
                     },
-                    "merqury": {
+                    "merqury/merqury": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/merqury/merqury.diff"
+                        "git_sha": "5f7195c5a3e889a03c31c43aec72a65f8b1f9282",
+                        "installed_by": ["modules"]
                     },
                     "meryl/count": {
                         "branch": "master",
diff --git a/modules/nf-core/merqury/merqury.diff b/modules/nf-core/merqury/merqury.diff
deleted file mode 100644
index e97121d7..00000000
--- a/modules/nf-core/merqury/merqury.diff
+++ /dev/null
@@ -1,44 +0,0 @@
-Changes in module 'nf-core/merqury'
---- modules/nf-core/merqury/main.nf
-+++ modules/nf-core/merqury/main.nf
-@@ -26,6 +26,7 @@
-     tuple val(meta), path("${prefix}.qv")        , emit: assembly_qv
-     tuple val(meta), path("${prefix}.*.qv")      , emit: scaffold_qv
-     tuple val(meta), path("*.hist.ploidy")       , emit: read_ploidy
-+    tuple val(meta), path("*.hapmers.blob.png")  , emit: hapmers_blob_png           , optional: true
-     path "versions.yml"                          , emit: versions
- 
-     when:
-@@ -56,4 +57,30 @@
-         merqury: $VERSION
-     END_VERSIONS
-     """
-+
-+    stub:
-+    prefix = task.ext.prefix ?: "${meta.id}"
-+    def VERSION = 1.3
-+    """
-+    touch ${prefix}_only.bed
-+    touch ${prefix}_only.wig
-+    touch ${prefix}.completeness.stats
-+    touch ${prefix}.dist_only.hist
-+    touch ${prefix}.spectra-cn.fl.png
-+    touch ${prefix}.spectra-cn.hist
-+    touch ${prefix}.spectra-cn.ln.png
-+    touch ${prefix}.spectra-cn.st.png
-+    touch ${prefix}.spectra-asm.fl.png
-+    touch ${prefix}.spectra-asm.hist
-+    touch ${prefix}.spectra-asm.ln.png
-+    touch ${prefix}.spectra-asm.st.png
-+    touch ${prefix}.qv
-+    touch ${prefix}.${prefix}.qv
-+    touch ${prefix}.hist.ploidy
-+
-+    cat <<-END_VERSIONS > versions.yml
-+    "${task.process}":
-+        merqury: $VERSION
-+    END_VERSIONS
-+    """
- }
-
-************************************************************
diff --git a/modules/nf-core/merqury/environment.yml b/modules/nf-core/merqury/merqury/environment.yml
similarity index 80%
rename from modules/nf-core/merqury/environment.yml
rename to modules/nf-core/merqury/merqury/environment.yml
index b73f4aea..722c47c1 100644
--- a/modules/nf-core/merqury/environment.yml
+++ b/modules/nf-core/merqury/merqury/environment.yml
@@ -1,4 +1,4 @@
-name: merqury
+name: merqury_merqury
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/merqury/main.nf b/modules/nf-core/merqury/merqury/main.nf
similarity index 88%
rename from modules/nf-core/merqury/main.nf
rename to modules/nf-core/merqury/merqury/main.nf
index 0e0d4369..ca8795a9 100644
--- a/modules/nf-core/merqury/main.nf
+++ b/modules/nf-core/merqury/merqury/main.nf
@@ -1,7 +1,8 @@
-process MERQURY {
+process MERQURY_MERQURY {
     tag "$meta.id"
     label 'process_low'
 
+    // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/merqury:1.3--hdfd78af_1':
@@ -35,7 +36,7 @@ process MERQURY {
     script:
     // def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = 1.3
+    def VERSION = 1.3 // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     # Nextflow changes the container --entrypoint to /bin/bash (container default entrypoint: /usr/local/env-execute)
     # Check for container variable initialisation script and source it.
@@ -60,7 +61,7 @@ process MERQURY {
 
     stub:
     prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = 1.3
+    def VERSION = 1.3 // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     touch ${prefix}_only.bed
     touch ${prefix}_only.wig
diff --git a/modules/nf-core/merqury/meta.yml b/modules/nf-core/merqury/merqury/meta.yml
similarity index 94%
rename from modules/nf-core/merqury/meta.yml
rename to modules/nf-core/merqury/merqury/meta.yml
index 88825386..19cb11b3 100644
--- a/modules/nf-core/merqury/meta.yml
+++ b/modules/nf-core/merqury/merqury/meta.yml
@@ -1,4 +1,4 @@
-name: "merqury"
+name: "merqury_merqury"
 description: k-mer based assembly evaluation.
 keywords:
   - "k-mer"
@@ -9,7 +9,7 @@ tools:
       description: "Evaluate genome assemblies with k-mers and more."
       tool_dev_url: "https://github.com/marbl/merqury"
       doi: "10.1186/s13059-020-02134-9"
-      licence: "['PUBLIC DOMAIN']"
+      licence: ["PUBLIC DOMAIN"]
 input:
   - meta:
       type: map
@@ -92,7 +92,12 @@ output:
       type: file
       description: "Ploidy estimate from read k-mer database"
       pattern: "*.hist.ploidy"
+  - hapmers_blob_png:
+      type: file
+      description: "Hap-mer blob plot"
+      pattern: "*.hapmers.blob.png"
 authors:
   - "@mahesh-panchal"
 maintainers:
   - "@mahesh-panchal"
+  - "@gallvp"
diff --git a/modules/nf-core/merqury/merqury/tests/main.nf.test b/modules/nf-core/merqury/merqury/tests/main.nf.test
new file mode 100644
index 00000000..34c7b46c
--- /dev/null
+++ b/modules/nf-core/merqury/merqury/tests/main.nf.test
@@ -0,0 +1,189 @@
+nextflow_process {
+
+    name "Test Process MERQURY_MERQURY"
+    script "../main.nf"
+    process "MERQURY_MERQURY"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "merqury"
+    tag "merqury/merqury"
+    tag "meryl/count"
+    tag "meryl/unionsum"
+
+    setup {
+        run("MERYL_COUNT") {
+            script "../../../meryl/count"
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['homo_sapiens']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                ]
+                input[1] = 21
+                """
+            }
+        }
+
+        run("MERYL_UNIONSUM") {
+            script "../../../meryl/unionsum"
+            process {
+                """
+                input[0] = MERYL_COUNT.out.meryl_db
+                input[1] = 21
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens-genome") {
+
+        when {
+            process {
+                """
+                assembly = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[0] = MERYL_UNIONSUM.out.meryl_db.join( Channel.value( assembly ) )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.assembly_only_kmers_bed,
+                    process.out.assembly_only_kmers_wig,
+                    process.out.stats,
+                    process.out.dist_hist,
+                    process.out.spectra_cn_hist,
+                    process.out.spectra_asm_hist,
+                    process.out.assembly_qv,
+                    process.out.scaffold_qv,
+                    process.out.read_ploidy,
+                    process.out.versions,
+                    file(process.out.spectra_cn_fl_png[0][1]).name,
+                    file(process.out.spectra_cn_ln_png[0][1]).name,
+                    file(process.out.spectra_cn_st_png[0][1]).name,
+                    file(process.out.spectra_asm_fl_png[0][1]).name,
+                    file(process.out.spectra_asm_ln_png[0][1]).name,
+                    file(process.out.spectra_asm_st_png[0][1]).name
+                    ).match()
+                },
+                { assert process.out.hapmers_blob_png == [] }
+            )
+        }
+
+    }
+
+    test("homo_sapiens-genome-trio") {
+
+        setup {
+            run("MERYL_COUNT", alias: "MATERNAL_COUNT") {
+                script "../../../meryl/count"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'test', single_end:false ], // meta map
+                        [
+                            file(params.test_data['homo_sapiens']['illumina']['test2_1_fastq_gz'], checkIfExists: true)
+                        ]
+                    ]
+                    input[1] = 21
+                    """
+                }
+            }
+
+            run("MERYL_COUNT", alias: "PATERNAL_COUNT") {
+                script "../../../meryl/count"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'test', single_end:false ], // meta map
+                        [
+                            file(params.test_data['homo_sapiens']['illumina']['test2_2_fastq_gz'], checkIfExists: true)
+                        ]
+                    ]
+                    input[1] = 21
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                ch_assembly = Channel.value([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ])
+
+                ch_input = MERYL_UNIONSUM.out.meryl_db
+                            | join( MATERNAL_COUNT.out.meryl_db )
+                            | join( PATERNAL_COUNT.out.meryl_db )
+                            | join( ch_assembly )
+                            | map { meta, meryl, mat_meryl, pat_meryl, fasta -> [ meta, [ meryl, mat_meryl, pat_meryl ], fasta ] }
+                input[0] = ch_input
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.assembly_only_kmers_bed,
+                    process.out.assembly_only_kmers_wig,
+                    process.out.stats,
+                    process.out.dist_hist,
+                    process.out.spectra_cn_hist,
+                    process.out.spectra_asm_hist,
+                    process.out.assembly_qv,
+                    process.out.scaffold_qv,
+                    process.out.read_ploidy,
+                    process.out.versions,
+                    process.out.spectra_cn_fl_png[0][1] .collect { file(it).name }.join(','),
+                    process.out.spectra_cn_ln_png[0][1] .collect { file(it).name }.join(','),
+                    process.out.spectra_cn_st_png[0][1] .collect { file(it).name }.join(','),
+                    file(process.out.spectra_asm_fl_png[0][1]).name,
+                    file(process.out.spectra_asm_ln_png[0][1]).name,
+                    file(process.out.spectra_asm_st_png[0][1]).name,
+                    file(process.out.hapmers_blob_png[0][1]).name,
+                    ).match()
+                }
+            )
+        }
+
+    }
+
+    test("homo_sapiens-genome-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                assembly = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                input[0] = MERYL_UNIONSUM.out.meryl_db.join( Channel.value( assembly ) )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/merqury/merqury/tests/main.nf.test.snap b/modules/nf-core/merqury/merqury/tests/main.nf.test.snap
new file mode 100644
index 00000000..d96fa7ef
--- /dev/null
+++ b/modules/nf-core/merqury/merqury/tests/main.nf.test.snap
@@ -0,0 +1,494 @@
+{
+    "homo_sapiens-genome-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_only.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.ln.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "11": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.st.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "13": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "14": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.hist.ploidy:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "15": [
+                    
+                ],
+                "16": [
+                    "versions.yml:md5,825a4c61369638389227eee16dfb08b5"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.dist_only.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.fl.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.ln.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.st.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.fl.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "assembly_only_kmers_bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "assembly_only_kmers_wig": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_only.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "assembly_qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "dist_hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.dist_only.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "hapmers_blob_png": [
+                    
+                ],
+                "read_ploidy": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.hist.ploidy:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "scaffold_qv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.test.qv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_asm_fl_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.fl.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_asm_hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_asm_ln_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.ln.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_asm_st_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-asm.st.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_cn_fl_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.fl.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_cn_hist": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.hist:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_cn_ln_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.ln.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spectra_cn_st_png": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.spectra-cn.st.png:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,825a4c61369638389227eee16dfb08b5"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-22T21:00:35.907142"
+    },
+    "homo_sapiens-genome": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "genome_only.bed:md5,b611f22cde0e410a2ca07c1eefd042d3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "genome_only.wig:md5,19cf44989af72af597ef80d3489b4882"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.completeness.stats:md5,d923bf7c98342625d022b2c32be8dd3a"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.dist_only.hist:md5,e2e6b54b0febef1f0fcf24cd2afd0b7a"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.genome.spectra-cn.hist:md5,6140a138ba47cb2b97814c93f80b2575"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.spectra-asm.hist:md5,541c9d1f87ab5c44df5e9e0acc440f8d"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.qv:md5,6e04952bc182221c8b9e242dc3298808"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.genome.qv:md5,c554315aabcc4207c367805cf3090da3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.unionsum.hist.ploidy:md5,16cf2fd89ed870c9cceb269e8430169a"
+                ]
+            ],
+            [
+                "versions.yml:md5,825a4c61369638389227eee16dfb08b5"
+            ],
+            "test.genome.spectra-cn.fl.png",
+            "test.genome.spectra-cn.ln.png",
+            "test.genome.spectra-cn.st.png",
+            "test.spectra-asm.fl.png",
+            "test.spectra-asm.ln.png",
+            "test.spectra-asm.st.png"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-22T21:52:39.004978"
+    },
+    "homo_sapiens-genome-trio": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "genome_only.bed:md5,b611f22cde0e410a2ca07c1eefd042d3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "genome_only.wig:md5,19cf44989af72af597ef80d3489b4882"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.completeness.stats:md5,58b3933129832d64652babd80688eec3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.dist_only.hist:md5,e2e6b54b0febef1f0fcf24cd2afd0b7a"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.genome.spectra-cn.hist:md5,6140a138ba47cb2b97814c93f80b2575"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.spectra-asm.hist:md5,541c9d1f87ab5c44df5e9e0acc440f8d"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.qv:md5,6e04952bc182221c8b9e242dc3298808"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.genome.qv:md5,c554315aabcc4207c367805cf3090da3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.unionsum.hist.ploidy:md5,16cf2fd89ed870c9cceb269e8430169a"
+                ]
+            ],
+            [
+                "versions.yml:md5,825a4c61369638389227eee16dfb08b5"
+            ],
+            "test.genome.read.test2_1.spectra-cn.fl.png,test.genome.read.test2_2.spectra-cn.fl.png,test.genome.spectra-cn.fl.png",
+            "test.genome.read.test2_1.spectra-cn.ln.png,test.genome.read.test2_2.spectra-cn.ln.png,test.genome.spectra-cn.ln.png",
+            "test.genome.read.test2_1.spectra-cn.st.png,test.genome.read.test2_2.spectra-cn.st.png,test.genome.spectra-cn.st.png",
+            "test.spectra-asm.fl.png",
+            "test.spectra-asm.ln.png",
+            "test.spectra-asm.st.png",
+            "test.hapmers.blob.png"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-23T10:37:10.601154"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/merqury/merqury/tests/tags.yml b/modules/nf-core/merqury/merqury/tests/tags.yml
new file mode 100644
index 00000000..af157f18
--- /dev/null
+++ b/modules/nf-core/merqury/merqury/tests/tags.yml
@@ -0,0 +1,2 @@
+merqury/merqury:
+  - "modules/nf-core/merqury/merqury/**"
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 282e5c6d..d939fe3c 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -28,7 +28,7 @@ include { MERYL_UNIONSUM as MAT_UNIONSUM    } from '../modules/nf-core/meryl/uni
 include { MERYL_COUNT as PAT_MERYL_COUNT    } from '../modules/nf-core/meryl/count/main'
 include { MERYL_UNIONSUM as PAT_UNIONSUM    } from '../modules/nf-core/meryl/unionsum/main'
 include { MERQURY_HAPMERS                   } from '../modules/pfr/merqury/hapmers/main'
-include { MERQURY                           } from '../modules/nf-core/merqury/main'
+include { MERQURY_MERQURY                   } from '../modules/nf-core/merqury/main'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
 include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as FETCHNGS  } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'
@@ -703,20 +703,20 @@ workflow ASSEMBLYQC {
                                                 ]
                                             }
 
-    // MODULE: MERQURY
+    // MODULE: MERQURY_MERQURY
     ch_merqury_inputs                       = ch_meryl_all
                                             | join(
                                                 ch_reads_assemblies
                                                 | map { meta, fq, fastas -> [ meta, fastas ] }
                                             )
 
-    MERQURY ( ch_merqury_inputs )
+    MERQURY_MERQURY ( ch_merqury_inputs )
 
-    ch_merqury_qv                           = MERQURY.out.assembly_qv
-    ch_merqury_stats                        = MERQURY.out.stats
-    ch_merqury_spectra_cn_fl_png            = MERQURY.out.spectra_cn_fl_png
-    ch_merqury_spectra_asm_fl_png           = MERQURY.out.spectra_asm_fl_png
-    ch_hapmers_blob_png                     = MERQURY.out.hapmers_blob_png
+    ch_merqury_qv                           = MERQURY_MERQURY.out.assembly_qv
+    ch_merqury_stats                        = MERQURY_MERQURY.out.stats
+    ch_merqury_spectra_cn_fl_png            = MERQURY_MERQURY.out.spectra_cn_fl_png
+    ch_merqury_spectra_asm_fl_png           = MERQURY_MERQURY.out.spectra_asm_fl_png
+    ch_hapmers_blob_png                     = MERQURY_MERQURY.out.hapmers_blob_png
 
     ch_merqury_outputs                      = ch_merqury_qv
                                             | mix(ch_merqury_stats)

From 38cdee7ddd90a32df66bf663b55dbf48c3f8fa7e Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 27 May 2024 17:35:56 +1200
Subject: [PATCH 53/76] Fixed merqury/merqury import path

---
 workflows/assemblyqc.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index d939fe3c..4f830f52 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -28,7 +28,7 @@ include { MERYL_UNIONSUM as MAT_UNIONSUM    } from '../modules/nf-core/meryl/uni
 include { MERYL_COUNT as PAT_MERYL_COUNT    } from '../modules/nf-core/meryl/count/main'
 include { MERYL_UNIONSUM as PAT_UNIONSUM    } from '../modules/nf-core/meryl/unionsum/main'
 include { MERQURY_HAPMERS                   } from '../modules/pfr/merqury/hapmers/main'
-include { MERQURY_MERQURY                   } from '../modules/nf-core/merqury/main'
+include { MERQURY_MERQURY                   } from '../modules/nf-core/merqury/merqury/main'
 include { CREATEREPORT                      } from '../modules/local/createreport'
 
 include { FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS as FETCHNGS  } from '../subworkflows/nf-core/fastq_download_prefetch_fasterqdump_sratools/main'

From 57518fcc961b2cb971a97212dc8bcbc98e47d6f2 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 27 May 2024 17:41:11 +1200
Subject: [PATCH 54/76] Fixed a typo

---
 workflows/assemblyqc.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 4f830f52..3f0fce8f 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -724,7 +724,7 @@ workflow ASSEMBLYQC {
                                             | mix(ch_merqury_spectra_asm_fl_png)
                                             | mix(ch_hapmers_blob_png)
                                             | flatMap { meta, data -> data }
-    ch_versions                             = ch_versions.mix(MERQURY.out.versions.first())
+    ch_versions                             = ch_versions.mix(MERQURY_MERQURY.out.versions.first())
 
     // Collate and save software versions
     ch_versions                             = ch_versions

From cab93a33d481758eafdaf7403b0dff660ffd65aa Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 27 May 2024 18:09:45 +1200
Subject: [PATCH 55/76] Added JAD labels to tests/stub

---
 README.md                     |  4 ++--
 tests/stub/JAD.seq.labels.tsv | 10 ++++++++++
 2 files changed, 12 insertions(+), 2 deletions(-)
 create mode 100644 tests/stub/JAD.seq.labels.tsv

diff --git a/README.md b/README.md
index 60b05536..8eebe643 100644
--- a/README.md
+++ b/README.md
@@ -63,8 +63,8 @@ flowchart LR
   MERQURY --> REPORT
 ```
 
-- [FASTA VALIDATION](https://github.com/GallVp/fasta_validator)
-- [GFF3 VALIDATION](https://genometools.org/tools/gt_gff3validator.html)
+- [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator): FASTA validation
+- [GenomeTools GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation
 - [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics
 - [GENOMETOOLS GT STAT](https://genometools.org/tools/gt_stat.html): Annotation statistics
 - [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail
diff --git a/tests/stub/JAD.seq.labels.tsv b/tests/stub/JAD.seq.labels.tsv
new file mode 100644
index 00000000..7d957f9c
--- /dev/null
+++ b/tests/stub/JAD.seq.labels.tsv
@@ -0,0 +1,10 @@
+JADWOS010000003.1	JAD1
+JADWOS010000004.1	JAD2
+JADWOS010000005.1	JAD3
+JADWOS010000006.1	JAD4
+JADWOS010000007.1	JAD5
+JADWOS010000008.1	JAD6
+JADWOS010000009.1	JAD7
+JADWOS010000001.1	JAD8
+JADWOS010000010.1	JAD9
+JADWOS010000011.1	JAD10

From f1d56428ecbc77c5bc48daf132b0945efd0d8d9c Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 27 May 2024 20:48:17 +1200
Subject: [PATCH 56/76] Now detecting duplicate sequences with SEQKIT/RMDUP

---
 CHANGELOG.md                                  |   2 +
 .../parsers/fasta_validate_parser.py          |  10 +
 conf/modules.config                           |   5 +
 docs/parameters.md                            |   6 +
 modules/nf-core/seqkit/rmdup/environment.yml  |   9 +
 modules/nf-core/seqkit/rmdup/main.nf          |  66 +++++
 modules/nf-core/seqkit/rmdup/meta.yml         |  50 ++++
 .../nf-core/seqkit/rmdup/tests/main.nf.test   | 173 ++++++++++++
 .../seqkit/rmdup/tests/main.nf.test.snap      | 247 ++++++++++++++++++
 modules/nf-core/seqkit/rmdup/tests/tags.yml   |   2 +
 nextflow.config                               |   3 +
 nextflow_schema.json                          |  17 ++
 tests/invalid/assemblysheet.csv               |   9 +-
 tests/invalid/dupseq.fsa.gz                   | Bin 0 -> 47 bytes
 workflows/assemblyqc.nf                       |  38 ++-
 15 files changed, 630 insertions(+), 7 deletions(-)
 create mode 100644 modules/nf-core/seqkit/rmdup/environment.yml
 create mode 100644 modules/nf-core/seqkit/rmdup/main.nf
 create mode 100644 modules/nf-core/seqkit/rmdup/meta.yml
 create mode 100644 modules/nf-core/seqkit/rmdup/tests/main.nf.test
 create mode 100644 modules/nf-core/seqkit/rmdup/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/seqkit/rmdup/tests/tags.yml
 create mode 100644 tests/invalid/dupseq.fsa.gz

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 724eb313..fcd8277d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -23,6 +23,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 14. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
 15. Added PLOTSR [#77](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/77)
 16. Added [JADWOS01](https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_016859245.1/) assembly to xrefsheet for successfully running PLOTSR.
+17. Manually copied [SEQKIT/RMDUP](../nf-modules/modules/nf-core/seqkit/rmdup)
+18. Now detecting duplicate sequences with `SEQKIT/RMDUP` [#64](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/64)
 
 ### `Fixed`
 
diff --git a/bin/report_modules/parsers/fasta_validate_parser.py b/bin/report_modules/parsers/fasta_validate_parser.py
index 786978ec..e572348f 100644
--- a/bin/report_modules/parsers/fasta_validate_parser.py
+++ b/bin/report_modules/parsers/fasta_validate_parser.py
@@ -17,6 +17,16 @@ def parse_fasta_validate_folder(folder_name="fastavalidator_logs"):
     data = {"FASTA_VALIDATE": []}
 
     for log_path in list_of_log_files:
+
+        if str(log_path).endswith(".seqkit.rmdup.log"):
+            data["FASTA_VALIDATE"].append(
+                {
+                    "hap": os.path.basename(log_path).replace(".seqkit.rmdup.log", ""),
+                    "validation_log": "FASTA validation failed due to presence of duplicate sequences",
+                }
+            )
+            continue
+
         with open(log_path, "r") as f:
             log_lines = [f"<p class='section-para' >{l}</p>" for l in f.readlines()]
 
diff --git a/conf/modules.config b/conf/modules.config
index 5082199d..3140cae1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -12,6 +12,11 @@
 
 process {
 
+    withName: SEQKIT_RMDUP {
+        ext.args = '--by-seq --ignore-case'
+        ext.prefix = { "${meta.id}.seqkit.rmdup" }
+    }
+
     withName: '.*:GFF3_GT_GFF3_GFF3VALIDATOR_STAT:GT_GFF3' {
         ext.args = '-tidy -retainids'
     }
diff --git a/docs/parameters.md b/docs/parameters.md
index 07cf3d4c..3b763b7c 100644
--- a/docs/parameters.md
+++ b/docs/parameters.md
@@ -10,6 +10,12 @@ A NextFlow pipeline which evaluates assembly quality with multiple QC tools and
 | `outdir`  | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure. | `string` | ./results | True     |        |
 | `email`   | Email address for completion summary.                                                                                    | `string` |           |          |        |
 
+## Validation options
+
+| Parameter                   | Description                                       | Type      | Default | Required | Hidden |
+| --------------------------- | ------------------------------------------------- | --------- | ------- | -------- | ------ |
+| `check_sequence_duplicates` | Check for duplicate sequences in fasta validation | `boolean` | True    |          |        |
+
 ## General stats options
 
 | Parameter                    | Description                                                             | Type      | Default | Required | Hidden |
diff --git a/modules/nf-core/seqkit/rmdup/environment.yml b/modules/nf-core/seqkit/rmdup/environment.yml
new file mode 100644
index 00000000..6cb8fe9d
--- /dev/null
+++ b/modules/nf-core/seqkit/rmdup/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "seqkit_rmdup"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - "bioconda::seqkit=2.8.1"
diff --git a/modules/nf-core/seqkit/rmdup/main.nf b/modules/nf-core/seqkit/rmdup/main.nf
new file mode 100644
index 00000000..410bb836
--- /dev/null
+++ b/modules/nf-core/seqkit/rmdup/main.nf
@@ -0,0 +1,66 @@
+process SEQKIT_RMDUP {
+    tag "$meta.id"
+    label 'process_low'
+    // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/seqkit:2.8.1--h9ee0642_0':
+        'biocontainers/seqkit:2.8.1--h9ee0642_0' }"
+
+    input:
+    tuple val(meta), path(fastx)
+
+    output:
+    tuple val(meta), path("${prefix}.${extension}") , emit: fastx
+    tuple val(meta), path("*.log")                  , emit: log
+    path "versions.yml"                             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args        = task.ext.args ?: ''
+    prefix          = task.ext.prefix ?: "${meta.id}"
+    extension       = "fastq"
+    if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) {
+        extension   = "fasta"
+    }
+    extension       = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension
+    // SeqKit/rmdup takes care of compressing the output: https://bioinf.shenwei.me/seqkit/usage/#rmdup
+    if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    seqkit \\
+        rmdup \\
+        --threads $task.cpus \\
+        $args \\
+        $fastx \\
+        -o ${prefix}.${extension} \\
+        2> >(tee ${prefix}.log >&2)
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        seqkit: \$(seqkit version | cut -d' ' -f2)
+    END_VERSIONS
+    """
+
+    stub:
+    prefix          = task.ext.prefix ?: "${meta.id}"
+    extension       = "fastq"
+    if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) {
+        extension   = "fasta"
+    }
+    extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension
+    if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    touch ${prefix}.${extension}
+    echo \\
+        '[INFO] 0 duplicated records removed' \\
+        > ${prefix}.log
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        seqkit: \$(seqkit version | cut -d' ' -f2)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/seqkit/rmdup/meta.yml b/modules/nf-core/seqkit/rmdup/meta.yml
new file mode 100644
index 00000000..d0addd45
--- /dev/null
+++ b/modules/nf-core/seqkit/rmdup/meta.yml
@@ -0,0 +1,50 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "seqkit_rmdup"
+description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...)
+keywords:
+  - genomics
+  - fasta
+  - fastq
+  - remove
+  - duplicates
+tools:
+  - "seqkit":
+      description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation"
+      homepage: "https://bioinf.shenwei.me/seqkit/"
+      documentation: "https://bioinf.shenwei.me/seqkit/usage/"
+      tool_dev_url: "https://github.com/shenwei356/seqkit"
+      doi: "10.1371/journal.pone.0163962"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - fastx:
+      type: file
+      description: Input fasta/fastq file
+      pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1' ]`
+  - fastx:
+      type: file
+      description: Output fasta/fastq file
+      pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}"
+  - log:
+      type: file
+      description: Log containing information regarding removed duplicates
+      pattern: "*.log"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@GallVp"
+maintainers:
+  - "@GallVp"
diff --git a/modules/nf-core/seqkit/rmdup/tests/main.nf.test b/modules/nf-core/seqkit/rmdup/tests/main.nf.test
new file mode 100644
index 00000000..1ba531e7
--- /dev/null
+++ b/modules/nf-core/seqkit/rmdup/tests/main.nf.test
@@ -0,0 +1,173 @@
+nextflow_process {
+
+    name "Test Process SEQKIT_RMDUP"
+    script "../main.nf"
+    process "SEQKIT_RMDUP"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "seqkit"
+    tag "seqkit/rmdup"
+
+    test("sarscov2-genome_fasta") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert path(process.out.log[0][1]).text.contains('0 duplicated records removed') }
+            )
+        }
+
+    }
+
+    test("repeated-fasta") {
+        when {
+            process {
+                """
+                def repeated_fasta = file('repeated.fasta')
+                repeated_fasta.text = '>A\\nAGCTAGCTAGCT\\n>B\\nAGCTAGCTAGCT\\n>A\\nAGCTAGCTAGCT'
+
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    repeated_fasta
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert path(process.out.log[0][1]).text.contains('1 duplicated records removed') }
+            )
+        }
+
+    }
+
+    test("sarscov2-genome_fasta_gz") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert path(process.out.log[0][1]).text.contains('0 duplicated records removed') }
+            )
+        }
+
+    }
+
+    test("sarscov2-test_1_fastq_gz") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert path(process.out.log[0][1]).text.contains('0 duplicated records removed') }
+            )
+        }
+
+    }
+
+    test("file_name_conflict-fail_with_error") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test_1' ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert !process.success },
+                { assert process.stdout.toString().contains("Input and output names are the same") }
+            )
+        }
+
+    }
+
+    test("sarscov2-genome_fasta-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert path(process.out.log[0][1]).text.contains('0 duplicated records removed') }
+            )
+        }
+
+    }
+
+    test("file_name_conflict-fail_with_error-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'genome' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert !process.success },
+                { assert process.stdout.toString().contains("Input and output names are the same") }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/seqkit/rmdup/tests/main.nf.test.snap b/modules/nf-core/seqkit/rmdup/tests/main.nf.test.snap
new file mode 100644
index 00000000..68c415cd
--- /dev/null
+++ b/modules/nf-core/seqkit/rmdup/tests/main.nf.test.snap
@@ -0,0 +1,247 @@
+{
+    "sarscov2-genome_fasta-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,cf833211befdf890bb6b2a3cd0b91853"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ],
+                "fastx": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,cf833211befdf890bb6b2a3cd0b91853"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-27T19:40:01.6034"
+    },
+    "sarscov2-test_1_fastq_gz": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,a41135cfe024baaf42f135583fe73f0d"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ],
+                "fastx": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,a41135cfe024baaf42f135583fe73f0d"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-27T19:37:48.551195"
+    },
+    "sarscov2-genome_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,a41135cfe024baaf42f135583fe73f0d"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ],
+                "fastx": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,a41135cfe024baaf42f135583fe73f0d"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-27T19:37:38.821528"
+    },
+    "sarscov2-genome_fasta_gz": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,a41135cfe024baaf42f135583fe73f0d"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ],
+                "fastx": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,a41135cfe024baaf42f135583fe73f0d"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-27T19:37:43.723054"
+    },
+    "repeated-fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,7510a742291241e7d7556bf720caf65c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,314c0aaef0f832a217a3f6ce3f8bc117"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ],
+                "fastx": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,7510a742291241e7d7556bf720caf65c"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.log:md5,314c0aaef0f832a217a3f6ce3f8bc117"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2b8da3c114c2bd1c6606030df55b6aa"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-27T19:52:34.545807"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/seqkit/rmdup/tests/tags.yml b/modules/nf-core/seqkit/rmdup/tests/tags.yml
new file mode 100644
index 00000000..e732db38
--- /dev/null
+++ b/modules/nf-core/seqkit/rmdup/tests/tags.yml
@@ -0,0 +1,2 @@
+seqkit/rmdup:
+  - "modules/nf-core/seqkit/rmdup/**"
diff --git a/nextflow.config b/nextflow.config
index 23837203..e3c9663b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -12,6 +12,9 @@ params {
     // Input options
     input                               = null
 
+    // Validation options
+    check_sequence_duplicates           = true
+
     // Assemblathon stats options
     assemblathon_stats_n_limit          = 100
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f6b73930..1fee6de3 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -37,6 +37,20 @@
             },
             "required": ["input", "outdir"]
         },
+        "validation_options": {
+            "title": "Validation options",
+            "type": "object",
+            "description": "",
+            "default": "",
+            "properties": {
+                "check_sequence_duplicates": {
+                    "type": "boolean",
+                    "default": true,
+                    "fa_icon": "fas fa-question-circle",
+                    "description": "Check for duplicate sequences in fasta validation"
+                }
+            }
+        },
         "general_stats_options": {
             "title": "General stats options",
             "type": "object",
@@ -497,6 +511,9 @@
         {
             "$ref": "#/definitions/input_output_options"
         },
+        {
+            "$ref": "#/definitions/validation_options"
+        },
         {
             "$ref": "#/definitions/general_stats_options"
         },
diff --git a/tests/invalid/assemblysheet.csv b/tests/invalid/assemblysheet.csv
index de7e586f..93e81420 100644
--- a/tests/invalid/assemblysheet.csv
+++ b/tests/invalid/assemblysheet.csv
@@ -1,4 +1,5 @@
-tag,fasta,gff3,monoploid_ids,synteny_labels
-FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,,
-TT_2021a,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,,
-MISC,tests/invalid/invalid.fsa.gz,,,
+tag,fasta,gff3
+FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz
+TT_2021a,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz
+MISC,tests/invalid/invalid.fsa.gz
+DUPSEQ,tests/invalid/dupseq.fsa.gz
diff --git a/tests/invalid/dupseq.fsa.gz b/tests/invalid/dupseq.fsa.gz
new file mode 100644
index 0000000000000000000000000000000000000000..33cdb35420460158bcdded0d56f62936410e0efa
GIT binary patch
literal 47
zcmb2|=HQs@5|YNioKjj)oLZ=tR-DMNx%f#@Njab6v6wQyh$C|hk1`|~OI6u0FfafB
DgclF^

literal 0
HcmV?d00001

diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 3f0fce8f..61b873d2 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -9,8 +9,8 @@ include { softwareVersionsToYAML            } from '../subworkflows/nf-core/util
 include { GUNZIP as GUNZIP_FASTA            } from '../modules/nf-core/gunzip/main'
 include { GUNZIP as GUNZIP_GFF3             } from '../modules/nf-core/gunzip/main'
 include { FASTAVALIDATOR                    } from '../modules/nf-core/fastavalidator/main'
+include { SEQKIT_RMDUP                      } from '../modules/nf-core/seqkit/rmdup/main'
 include { FASTA_EXPLORE_SEARCH_PLOT_TIDK    } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main'
-
 include { GFF3_GT_GFF3_GFF3VALIDATOR_STAT   } from '../subworkflows/pfr/gff3_gt_gff3_gff3validator_stat/main'
 include { FCS_FCSADAPTOR                    } from '../modules/nf-core/fcs/fcsadaptor/main'
 include { NCBI_FCS_GX                       } from '../subworkflows/local/ncbi_fcs_gx'
@@ -125,10 +125,10 @@ workflow ASSEMBLYQC {
     // MODULE: FASTAVALIDATOR
     FASTAVALIDATOR ( ch_target_assembly )
 
-    ch_valid_target_assembly                = ch_target_assembly.join(FASTAVALIDATOR.out.success_log)
+    ch_fastavalidator_assembly              = ch_target_assembly.join(FASTAVALIDATOR.out.success_log)
                                             | map { meta, fasta, log -> [ meta, fasta ] }
 
-    ch_invalid_assembly_log                 = FASTAVALIDATOR.out.error_log
+    ch_fastavalidator_log                   = FASTAVALIDATOR.out.error_log
                                             | map { meta, error_log ->
                                                 log.warn("FASTA validation failed for ${meta.id}\n${error_log.text}")
 
@@ -137,6 +137,38 @@ workflow ASSEMBLYQC {
 
     ch_versions                             = ch_versions.mix(FASTAVALIDATOR.out.versions.first())
 
+    // MODULE: SEQKIT_RMDUP
+    ch_seqkit_rmdup_input                   = ! params.check_sequence_duplicates
+                                            ? Channel.empty()
+                                            : ch_fastavalidator_assembly
+    SEQKIT_RMDUP ( ch_seqkit_rmdup_input )
+
+    ch_valid_target_assembly                = params.check_sequence_duplicates
+                                            ? SEQKIT_RMDUP.out.log
+                                            | join(ch_seqkit_rmdup_input)
+                                            | map { meta, error_log, fasta ->
+                                                if ( error_log.text.contains('0 duplicated records removed') ) {
+                                                    return [ meta, fasta ]
+                                                }
+
+                                                log.warn("FASTA validation failed for ${meta.id} due to presence of duplicate sequences")
+                                                return null
+                                            }
+                                            : ch_fastavalidator_assembly
+
+    ch_invalid_assembly_log                 = ch_fastavalidator_log
+                                            | mix(
+                                                SEQKIT_RMDUP.out.log
+                                                | map { meta, error_log ->
+                                                    if ( error_log.text.contains('0 duplicated records removed') ) {
+                                                        return null
+                                                    }
+
+                                                    error_log
+                                                }
+                                            )
+    ch_versions                             = ch_versions.mix(SEQKIT_RMDUP.out.versions.first())
+
     // SUBWORKFLOW: GFF3_GT_GFF3_GFF3VALIDATOR_STAT
     GFF3_GT_GFF3_GFF3VALIDATOR_STAT (
         ch_assembly_gff3,

From 3837c5245dc1db3ec7c99d7872759221e0dd5e42 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 13:05:58 +1200
Subject: [PATCH 57/76] Now imported seqkit/rmdup from nf-core/modules

---
 CHANGELOG.md                                    |  7 +++----
 CITATION.cff                                    |  2 +-
 README.md                                       |  7 +++++--
 modules.json                                    |  5 +++++
 modules/nf-core/seqkit/rmdup/tests/main.nf.test | 12 ++++++------
 nextflow.config                                 |  2 +-
 6 files changed, 21 insertions(+), 14 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fcd8277d..025db0a1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0+dev - [27-May-2024]
+## v2.0.0-rc.1 - [28-May-2024]
 
 ### `Added`
 
@@ -22,9 +22,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 13. Replaced local `BUSCO` module with `FASTA_GXF_BUSCO_PLOT` sub-workflow [#75](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/75)
 14. Replaced local `NCBI_FCS_ADAPTOR` with nf-core module and updated to 0.5.0 which includes additional adaptors for PacBio and Nanopore technologies [#55](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/55)
 15. Added PLOTSR [#77](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/77)
-16. Added [JADWOS01](https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_016859245.1/) assembly to xrefsheet for successfully running PLOTSR.
-17. Manually copied [SEQKIT/RMDUP](../nf-modules/modules/nf-core/seqkit/rmdup)
-18. Now detecting duplicate sequences with `SEQKIT/RMDUP` [#64](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/64)
+16. Added [JADWOS01](https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_016859245.1/) assembly to xrefsheet for successfully running PLOTSR
+17. Now detecting duplicate sequences with `SEQKIT/RMDUP` [#64](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/64)
 
 ### `Fixed`
 
diff --git a/CITATION.cff b/CITATION.cff
index e704741a..f3d7d693 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -21,7 +21,7 @@ authors:
   - family-names: "Deng"
     given-names: "Cecilia"
 title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality"
-version: 2.0.0+dev
+version: 2.0.0-rc.1
 date-released: 2024-02-12
 url: "https://github.com/Plant-Food-Research-Open/assembly_qc"
 doi: 10.5281/zenodo.10647870
diff --git a/README.md b/README.md
index 8eebe643..c279d69e 100644
--- a/README.md
+++ b/README.md
@@ -63,7 +63,7 @@ flowchart LR
   MERQURY --> REPORT
 ```
 
-- [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator): FASTA validation
+- [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator) + [SEQKIT RMDUP](https://github.com/shenwei356/seqkit): FASTA validation
 - [GenomeTools GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation
 - [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics
 - [GENOMETOOLS GT STAT](https://genometools.org/tools/gt_stat.html): Annotation statistics
@@ -133,12 +133,14 @@ The pipeline uses nf-core modules contributed by following authors.
 <a href="https://github.com/drpatelh"><img src="https://github.com/drpatelh.png" width="50" height="50"></a>
 <a href="https://github.com/midnighter"><img src="https://github.com/midnighter.png" width="50" height="50"></a>
 <a href="https://github.com/mahesh-panchal"><img src="https://github.com/mahesh-panchal.png" width="50" height="50"></a>
+<a href="https://github.com/jfy133"><img src="https://github.com/jfy133.png" width="50" height="50"></a>
 <a href="https://github.com/adamrtalbot"><img src="https://github.com/adamrtalbot.png" width="50" height="50"></a>
 <a href="https://github.com/maxulysse"><img src="https://github.com/maxulysse.png" width="50" height="50"></a>
 <a href="https://github.com/matthdsm"><img src="https://github.com/matthdsm.png" width="50" height="50"></a>
 <a href="https://github.com/joseespinosa"><img src="https://github.com/joseespinosa.png" width="50" height="50"></a>
-<a href="https://github.com/jfy133"><img src="https://github.com/jfy133.png" width="50" height="50"></a>
 <a href="https://github.com/ewels"><img src="https://github.com/ewels.png" width="50" height="50"></a>
+<a href="https://github.com/sofstam"><img src="https://github.com/sofstam.png" width="50" height="50"></a>
+<a href="https://github.com/sateeshperi"><img src="https://github.com/sateeshperi.png" width="50" height="50"></a>
 <a href="https://github.com/priyanka-surana"><img src="https://github.com/priyanka-surana.png" width="50" height="50"></a>
 <a href="https://github.com/phue"><img src="https://github.com/phue.png" width="50" height="50"></a>
 <a href="https://github.com/muffato"><img src="https://github.com/muffato.png" width="50" height="50"></a>
@@ -147,6 +149,7 @@ The pipeline uses nf-core modules contributed by following authors.
 <a href="https://github.com/jvhagey"><img src="https://github.com/jvhagey.png" width="50" height="50"></a>
 <a href="https://github.com/joon-klaps"><img src="https://github.com/joon-klaps.png" width="50" height="50"></a>
 <a href="https://github.com/jeremy1805"><img src="https://github.com/jeremy1805.png" width="50" height="50"></a>
+<a href="https://github.com/heuermh"><img src="https://github.com/heuermh.png" width="50" height="50"></a>
 <a href="https://github.com/grst"><img src="https://github.com/grst.png" width="50" height="50"></a>
 <a href="https://github.com/friederikehanssen"><img src="https://github.com/friederikehanssen.png" width="50" height="50"></a>
 <a href="https://github.com/felixkrueger"><img src="https://github.com/felixkrueger.png" width="50" height="50"></a>
diff --git a/modules.json b/modules.json
index 2f81369d..2ba6be9e 100644
--- a/modules.json
+++ b/modules.json
@@ -190,6 +190,11 @@
                         "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5",
                         "installed_by": ["modules"]
                     },
+                    "seqkit/rmdup": {
+                        "branch": "master",
+                        "git_sha": "85a37a184b03960e4908c100af934def04466b6d",
+                        "installed_by": ["modules"]
+                    },
                     "seqkit/seq": {
                         "branch": "master",
                         "git_sha": "2be41ca2cc780eca4293d1b0dd3850b0b7ac40a3",
diff --git a/modules/nf-core/seqkit/rmdup/tests/main.nf.test b/modules/nf-core/seqkit/rmdup/tests/main.nf.test
index 1ba531e7..e990443f 100644
--- a/modules/nf-core/seqkit/rmdup/tests/main.nf.test
+++ b/modules/nf-core/seqkit/rmdup/tests/main.nf.test
@@ -15,7 +15,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
                 ]
                 """
             }
@@ -62,7 +62,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
                 ]
                 """
             }
@@ -84,7 +84,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
                 ]
                 """
             }
@@ -106,7 +106,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test_1' ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
                 ]
                 """
             }
@@ -130,7 +130,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
                 ]
                 """
             }
@@ -155,7 +155,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'genome' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
                 ]
                 """
             }
diff --git a/nextflow.config b/nextflow.config
index e3c9663b..f494a1c8 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -283,7 +283,7 @@ manifest {
     description     = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '2.0.0+dev'
+    version         = '2.0.0-rc.1'
     doi             = 'https://doi.org/10.5281/zenodo.10647870'
 }
 

From dc1e17620337b6d4f4db8fe0a8adf25839973bea Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 13:13:20 +1200
Subject: [PATCH 58/76] Updated modules and sub-workflows

---
 CHANGELOG.md                                  |  3 +-
 conf/modules.config                           |  3 ++
 modules.json                                  |  8 +--
 .../nf-core/fcs/fcsadaptor/tests/main.nf.test | 46 ++++++++++++++++-
 .../fcs/fcsadaptor/tests/main.nf.test.snap    | 49 ++++++++++++++++++-
 .../templates/restore_gff_ids.py              |  6 +--
 .../custom/restoregffids/tests/main.nf.test   |  2 +-
 .../restoregffids/tests/main.nf.test.snap     | 20 ++++++++
 .../templates/shorten_fasta_ids.py            |  8 +--
 .../custom/shortenfastaids/tests/main.nf.test | 14 ++----
 .../shortenfastaids/tests/main.nf.test.snap   | 47 ++++++++++++++++--
 modules/pfr/gt/stat/main.nf                   | 21 ++++++--
 modules/pfr/gt/stat/meta.yml                  |  2 +-
 modules/pfr/gt/stat/tests/main.nf.test        | 29 ++++++++++-
 modules/pfr/gt/stat/tests/main.nf.test.snap   | 22 +++++++--
 modules/pfr/gt/stat/tests/tags.yml            |  2 +-
 16 files changed, 238 insertions(+), 44 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 025db0a1..154aeeaf 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,7 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 4. Added a launcher script for local testing `local_assemblyqc`
 5. Added a custom `BUNDLELINKS` module which respects direction when bundling `DNADIFF` links [#82](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/82)
 6. Added the ability to create linear synteny plot in addition to the circos plot [#74](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/74)
-7. Updated modules and sub-workflows: `BWA/INDEX`, `BWA/MEM`, `CAT/CAT`, `CUSTOM/CHECKGFF3FASTACORRESPONDENCE`, `CUSTOM/RESTOREGFFIDS`, `CUSTOM/SHORTENFASTAIDS`, `GT/GFF3`, `GT/GFF3VALIDATOR`, `GT/STAT`, `LTRFINDER`, `LTRHARVEST`, `LTRRETRIEVER/LAI`, `LTRRETRIEVER/LTRRETRIEVER`, `SAMBLASTER`, `FASTA_LTRRETRIEVER_LAI`, `FASTQ_BWA_MEM_SAMBLASTER`, `GFF3_VALIDATE`, `CUSTOM/SRATOOLSNCBISETTINGS`, `FASTP`, `FASTQC`, `UNTAR`, `SEQKIT/SEQ`, `SEQKIT/SORT`, `FASTA_EXPLORE_SEARCH_PLOT_TIDK`
+7. Updated modules and sub-workflows: `BWA/INDEX`, `BWA/MEM`, `CAT/CAT`, , `CUSTOM/RESTOREGFFIDS`, `CUSTOM/SHORTENFASTAIDS`, `GT/GFF3`, `GT/GFF3VALIDATOR`, `GT/STAT`, `LTRFINDER`, `LTRHARVEST`, `LTRRETRIEVER/LAI`, `LTRRETRIEVER/LTRRETRIEVER`, `SAMBLASTER`, `FASTA_LTRRETRIEVER_LAI`, `FASTQ_BWA_MEM_SAMBLASTER`, `GFF3_VALIDATE`, `CUSTOM/SRATOOLSNCBISETTINGS`, `FASTP`, `FASTQC`, `UNTAR`, `SEQKIT/SEQ`, `SEQKIT/SORT`, `FASTA_EXPLORE_SEARCH_PLOT_TIDK`
 8. Now the `contamination_stops_pipeline` flag allows the pipeline to continue if contamination is detected. It's default value is `true` [#54](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/54)
 9. Now fasta ids are sorted in natural order for the HiC module [#76](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/76)
 10. Now using `FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS` for SRA downloads
@@ -46,6 +46,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 1. Removed `CIRCOS_BUNDLELINKS` module
 2. Now the default value of `synteny_plot_1_vs_all` is false
+3. Replace module `CUSTOM/CHECKGFF3FASTACORRESPONDENCE` with a local groovy function in `GFF3_GT_GFF3_GFF3VALIDATOR_STAT` sub-workflow
 
 ## 1.4 - [04-Mar-2024]
 
diff --git a/conf/modules.config b/conf/modules.config
index 3140cae1..1b6ee2a1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -22,6 +22,9 @@ process {
     }
 
     withName: '.*:GFF3_GT_GFF3_GFF3VALIDATOR_STAT:GT_STAT' {
+
+        ext.prefix = { "${meta.id}.gt.stat" }
+
         ext.args = [
             '-genelengthdistri',
             '-genescoredistri',
diff --git a/modules.json b/modules.json
index 2ba6be9e..894d0ade 100644
--- a/modules.json
+++ b/modules.json
@@ -37,12 +37,12 @@
                     },
                     "custom/restoregffids": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "da7a50af5eaac9601e23f33e00bf24e61f9c27ab",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "custom/shortenfastaids": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "da7a50af5eaac9601e23f33e00bf24e61f9c27ab",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "gffread": {
@@ -62,7 +62,7 @@
                     },
                     "gt/stat": {
                         "branch": "main",
-                        "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e",
+                        "git_sha": "ffd39a616c84c814fdfb2a865e9fbbb06e5343d9",
                         "installed_by": ["gff3_gt_gff3_gff3validator_stat", "modules"]
                     },
                     "ltrfinder": {
@@ -162,7 +162,7 @@
                     },
                     "fcs/fcsadaptor": {
                         "branch": "master",
-                        "git_sha": "28adf00792d7dfd1b9ecbde9d7a8f22ec78ffcbb",
+                        "git_sha": "f0732b3df35850d3eb16feee0ba1c0192b05bbcb",
                         "installed_by": ["modules"]
                     },
                     "gunzip": {
diff --git a/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test
index 9d647490..13c80264 100644
--- a/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test
+++ b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test
@@ -26,8 +26,8 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert process.out.cleaned_assembly == [] },
-                { assert process.out.skipped_trims != null }, // md5sum for empty file not allowed in nf-core/tools 2.14.0
                 { assert snapshot(
+                    file(process.out.skipped_trims[0][1]).name,
                     process.out.adaptor_report,
                     process.out.pipeline_args,
                     process.out.versions,
@@ -38,6 +38,48 @@ nextflow_process {
         }
 
     }
+
+    test("sarscov2-genome_fasta-pacbio_adapters") {
+
+        when {
+            process {
+                """
+                def input_file_lines = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true).text.split('\\n')
+                def file_with_adapters = file('sarscov2_with_adapters.fasta')
+                file_with_adapters.text = (
+                    input_file_lines[0..<(input_file_lines.size()/2)]
+                    + ['ATCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGAT'] // Biosciences Blunt Adapter
+                    + input_file_lines[(input_file_lines.size()/2)..<(input_file_lines.size())]
+                    + ['AAAAAAAAAAAAAAAAAATTAACGGAGGAGGAGGA'] // Pacific Biosciences C2 Primer
+                    ).join('\\n')
+
+                input[0] = [
+                    [ id: 'test' ],
+                    file_with_adapters
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.cleaned_assembly,
+                    process.out.skipped_trims,
+                    process.out.adaptor_report,
+                    process.out.pipeline_args,
+                    process.out.versions,
+                    ).match()
+                },
+                { assert path(process.out.log[0][1]).text.contains('[step all_cleaned_fasta] completed success') },
+                { assert path(process.out.adaptor_report[0][1]).text.contains('Pacific Biosciences Blunt Adapter') },
+                { assert path(process.out.adaptor_report[0][1]).text.contains('Pacific Biosciences C2 Primer') }
+            )
+        }
+
+    }
+
     test("bacteroides_fragilis-genome_fna_gz-stub") {
 
         options "-stub"
@@ -65,7 +107,7 @@ nextflow_process {
                         process.out.skipped_trims[0][1]
                     ].collect { file(it).name }
                     ).match()
-                }, // md5sum for empty file not allowed in nf-core/tools 2.14.0
+                },
                 { assert snapshot(process.out.versions).match("stub_versions") }
             )
         }
diff --git a/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap
index ddc9ca06..3dc63939 100644
--- a/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap
+++ b/modules/nf-core/fcs/fcsadaptor/tests/main.nf.test.snap
@@ -1,6 +1,51 @@
 {
+    "sarscov2-genome_fasta-pacbio_adapters": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.cleaned_sequences.fa.gz:md5,a2f3d1c092463efff18ed3bb519a989e"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.skipped_trims.jsonl:md5,cbcdf071a7f61519e34d6b60c1aa9f51"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.fcs_adaptor_report.txt:md5,f515b272a270b63a059d74060a773d76"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.pipeline_args.yaml:md5,5df663124ae9536c596c5c572d4d4c45"
+                ]
+            ],
+            [
+                "versions.yml:md5,5cd44cb2972a42e74144373ce3307c7f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-05-27T12:10:37.17285"
+    },
     "bacteroides_fragilis-genome_fna_gz": {
         "content": [
+            "test.skipped_trims.jsonl",
             [
                 [
                     {
@@ -23,9 +68,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.1"
         },
-        "timestamp": "2024-05-09T11:54:31.333434"
+        "timestamp": "2024-05-27T12:10:01.657496"
     },
     "bacteroides_fragilis-genome_fna_gz-stub": {
         "content": [
diff --git a/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py b/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py
index d0699de0..2bde7abb 100755
--- a/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py
+++ b/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py
@@ -10,7 +10,7 @@
 def create_name_mapping_from_tsv(file_path):
     dictionary = {}
 
-    with open(file_path, "r") as tsv_file:
+    with open(file_path) as tsv_file:
         for line in tsv_file:
             columns = line.strip().split("\\t")
             if len(columns) != 2:
@@ -24,11 +24,11 @@ def create_name_mapping_from_tsv(file_path):
 
 def restore_gff3_ids(new_to_orig_ids, file_path, output_file_name):
     # Write versions
-    with open(f"versions.yml", "w") as f_versions:
+    with open("versions.yml", "w") as f_versions:
         f_versions.write('"${task.process}":\\n')
         f_versions.write(f"    python: {python_version()}\\n")
 
-    with open(file_path, "r") as input_gff3_file:
+    with open(file_path) as input_gff3_file:
         input_lines = input_gff3_file.readlines()
 
     with open(output_file_name, "w") as output_gff_file:
diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test b/modules/pfr/custom/restoregffids/tests/main.nf.test
index cc374b76..20aa85c9 100644
--- a/modules/pfr/custom/restoregffids/tests/main.nf.test
+++ b/modules/pfr/custom/restoregffids/tests/main.nf.test
@@ -54,7 +54,7 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert process.out.restored_ids_gff3 != null },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_stub") }
             )
         }
 
diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test.snap b/modules/pfr/custom/restoregffids/tests/main.nf.test.snap
index ffe43e74..ebe850a9 100644
--- a/modules/pfr/custom/restoregffids/tests/main.nf.test.snap
+++ b/modules/pfr/custom/restoregffids/tests/main.nf.test.snap
@@ -28,14 +28,34 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-12-07T13:49:30.047425"
     },
+    "versions_stub": {
+        "content": [
+            [
+                "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-10T15:26:11.66528"
+    },
     "versions": {
         "content": [
             [
                 "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-12-07T13:49:30.071175"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py
index 54f35bf6..67d6f565 100755
--- a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py
+++ b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py
@@ -1,11 +1,11 @@
 #!/usr/bin/env python3
 
 import re
-
-from Bio import SeqIO
 from importlib.metadata import version
 from platform import python_version
 
+from Bio import SeqIO
+
 # The input fasta file path
 fasta_file_path = "$fasta"
 output_files_prefix = "$prefix"
@@ -122,7 +122,7 @@ def shorten_id_by_pattern_replacement(patterns_dict, id):
 
     for pattern in matches_for_id:
         shortened_id = re.sub(
-            r"({})".format(re.escape(pattern)),
+            rf"({re.escape(pattern)})",
             patterns_dict[pattern],
             shortened_id,
         )
@@ -158,7 +158,7 @@ def fail_if_new_ids_not_valid(ids):
     input_ids = [x[0] for x in input_ids_and_descriptions]
 
     # Write versions
-    with open(f"versions.yml", "w") as f_versions:
+    with open("versions.yml", "w") as f_versions:
         f_versions.write('"${task.process}":\\n')
         f_versions.write(f"    python: {python_version()}\\n")
         f_versions.write(f"    biopython: {version('biopython')}\\n")
diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test b/modules/pfr/custom/shortenfastaids/tests/main.nf.test
index efff639e..4c6fbe58 100644
--- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test
+++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test
@@ -26,7 +26,6 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") },
                 { assert process.out.short_ids_fasta == [] },
                 { assert process.out.short_ids_tsv == [] }
             )
@@ -50,8 +49,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out).match() }
             )
         }
 
@@ -73,8 +71,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out).match() }
             )
         }
 
@@ -95,8 +92,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out).match() }
             )
         }
 
@@ -120,9 +116,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.versions).match("versions") },
-                { assert process.out.short_ids_fasta == [] },
-                { assert process.out.short_ids_tsv == [] }
+                { assert snapshot(process.out).match() }
             )
         }
 
diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
index 8fed1b9d..89b9483a 100644
--- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
+++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
@@ -42,15 +42,40 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-12-07T13:33:05.523745"
     },
-    "versions": {
+    "stub": {
         "content": [
-            [
-                "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
-            ]
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
+                ],
+                "short_ids_fasta": [
+                    
+                ],
+                "short_ids_tsv": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
+                ]
+            }
         ],
-        "timestamp": "2023-12-07T13:30:30.361527"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-10T15:27:57.244358"
     },
     "homo_sapiens-genome_fasta-no_change": {
         "content": [
@@ -75,6 +100,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-12-07T13:32:54.220188"
     },
     "homo_sapiens-genome2_fasta-length_change": {
@@ -120,6 +149,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-12-07T13:33:01.924483"
     },
     "sarscov2-genome_fasta-pattern_change": {
@@ -165,6 +198,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-12-07T13:32:58.12885"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/gt/stat/main.nf b/modules/pfr/gt/stat/main.nf
index 3308b562..0fbc936b 100644
--- a/modules/pfr/gt/stat/main.nf
+++ b/modules/pfr/gt/stat/main.nf
@@ -11,21 +11,32 @@ process GT_STAT {
     tuple val(meta), path(gff3)
 
     output:
-    tuple val(meta), path("*.gt.stat.yml")  , emit: stats
+    tuple val(meta), path("${prefix}.yml")  , emit: stats
     path "versions.yml"                     , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def args    = task.ext.args ?: ''
+    prefix      = task.ext.prefix ?: "${meta.id}"
     """
     gt \\
         stat \\
         $args \\
-        "$gff3" \\
-        > "${prefix}.gt.stat.yml"
+        $gff3 \\
+        > ${prefix}.yml
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //')
+    END_VERSIONS
+    """
+
+    stub:
+    prefix      = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.yml
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/pfr/gt/stat/meta.yml b/modules/pfr/gt/stat/meta.yml
index 203059a6..fa477f34 100644
--- a/modules/pfr/gt/stat/meta.yml
+++ b/modules/pfr/gt/stat/meta.yml
@@ -35,7 +35,7 @@ output:
   - stats:
       type: file
       description: Stats file in yaml format
-      pattern: "*.gt.stat.yml"
+      pattern: "*.yml"
   - versions:
       type: file
       description: File containing software versions
diff --git a/modules/pfr/gt/stat/tests/main.nf.test b/modules/pfr/gt/stat/tests/main.nf.test
index 8736d013..d1fe2f0d 100644
--- a/modules/pfr/gt/stat/tests/main.nf.test
+++ b/modules/pfr/gt/stat/tests/main.nf.test
@@ -16,7 +16,7 @@ nextflow_process {
                 """
                 input[0] = Channel.of(
                     "##gff-version 3" +
-                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true).getText().toLowerCase()
+                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true).text.toLowerCase()
                 )
                 .collectFile(name: 'sample.gff3', newLine: true)
                 .map { file -> [ [ id:'test' ], file ] }
@@ -28,7 +28,32 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(process.out).match() },
-                { assert path(process.out.stats.get(0).get(1)).getText().contains("cdss: 12") }
+                { assert path(process.out.stats[0][1]).text.contains("cdss: 12") }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id: 'test' ],
+                    file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.stats != null }, // md5sum for empty files not allowed by nf-core/tools 2.14.0
+                { assert snapshot(process.out.versions).match("stub_versions") }
             )
         }
 
diff --git a/modules/pfr/gt/stat/tests/main.nf.test.snap b/modules/pfr/gt/stat/tests/main.nf.test.snap
index 2fcfb8a8..481ceb1d 100644
--- a/modules/pfr/gt/stat/tests/main.nf.test.snap
+++ b/modules/pfr/gt/stat/tests/main.nf.test.snap
@@ -7,7 +7,7 @@
                         {
                             "id": "test"
                         },
-                        "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5"
+                        "test.yml:md5,ebba7831ddbf916b8bbea675ba8693b5"
                     ]
                 ],
                 "1": [
@@ -18,7 +18,7 @@
                         {
                             "id": "test"
                         },
-                        "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5"
+                        "test.yml:md5,ebba7831ddbf916b8bbea675ba8693b5"
                     ]
                 ],
                 "versions": [
@@ -26,6 +26,22 @@
                 ]
             }
         ],
-        "timestamp": "2023-11-29T11:34:48.057277"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T19:29:08.925383"
+    },
+    "stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-09T19:29:12.808817"
     }
 }
\ No newline at end of file
diff --git a/modules/pfr/gt/stat/tests/tags.yml b/modules/pfr/gt/stat/tests/tags.yml
index 46be6341..baef3b08 100644
--- a/modules/pfr/gt/stat/tests/tags.yml
+++ b/modules/pfr/gt/stat/tests/tags.yml
@@ -1,2 +1,2 @@
 gt/stat:
-  - "modules/pfr/gt/stat/**"
+  - "modules/nf-core/gt/stat/**"

From 04be085c4dc725dc5637ef23ffb1b5bb340c6583 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 15:10:29 +1200
Subject: [PATCH 59/76] Updated usage doc and pfr/params

---
 docs/usage.md   |  4 ++--
 pfr/params.json | 13 +++++++++----
 2 files changed, 11 insertions(+), 6 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index b7319586..a33a2c88 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -8,7 +8,7 @@ You will need to create an assemblysheet with information about the assemblies y
 - `fasta:` FASTA file
 - `gff3 [Optional]:` GFF3 annotation file if available
 - `monoploid_ids [Optional]:` A txt file listing the sequence IDs used to calculate LAI in monoploid mode if necessary. If the intent is to run LAI against all the sequences in an assembly, this file can be skipped for that assembly.
-- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence IDs (first column) and their labels for the synteny plots (second column) when performing synteny analysis. If a sequence ID is missing from this file, the corresponding sequence is excluded from the analysis.
+- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence IDs (first column) and their labels for the synteny plots (second column) when performing synteny analysis. If a sequence ID is missing from this file, the corresponding sequence is excluded from the analysis. If `synteny_labels` is not provided for an assembly, that assembly is excluded from the analysis.
 
 See the [Merqury](#merqury-k-mer-analysis) section For description of assemblysheet columns related to k-mer analysis with Merqury.
 
@@ -70,7 +70,7 @@ This section provides additional information for parameters. It does not list al
   - `fasta:` FASTA file
   - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column)
 
-- `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom.
+- `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom. If this parameter is `null`, the assemblies are ordered alphabetically. All assemblies from `input` and `synteny_xref_assemblies` are included by default. If an assembly is missing from this list, that assembly is excluded from the analysis.
 
 ### Merqury K-mer analysis
 
diff --git a/pfr/params.json b/pfr/params.json
index 1b7715be..cae8f7f9 100644
--- a/pfr/params.json
+++ b/pfr/params.json
@@ -1,12 +1,13 @@
 {
-    "input": "/workspace/assemblyqc/testdata/default/assemblysheet.csv",
+    "input": "/workspace/assemblyqc/testdata/v2/assemblysheet.csv",
+    "check_sequence_duplicates": true,
     "assemblathon_stats_n_limit": 100,
     "ncbi_fcs_adaptor_skip": false,
     "ncbi_fcs_adaptor_empire": "euk",
     "ncbi_fcs_gx_skip": false,
     "ncbi_fcs_gx_tax_id": 3750,
     "ncbi_fcs_gx_db_path": "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24",
-    "contamination_stops_pipeline": true,
+    "contamination_stops_pipeline": false,
     "busco_skip": false,
     "busco_mode": "geno",
     "busco_lineage_datasets": "embryophyta_odb10 eudicots_odb10",
@@ -23,14 +24,18 @@
     "hic_skip_fastqc": false,
     "hic_fastp_ext_args": "--qualified_quality_phred 20 --length_required 50",
     "synteny_skip": false,
-    "synteny_mummer_plot_type": "both",
+    "synteny_mummer_skip": false,
+    "synteny_plotsr_skip": false,
+    "synteny_xref_assemblies": "/workspace/assemblyqc/testdata/v2/xrefsheet.csv",
     "synteny_between_input_assemblies": true,
+    "synteny_mummer_plot_type": "both",
     "synteny_mummer_m2m_align": false,
     "synteny_mummer_max_gap": 1000000,
     "synteny_mummer_min_bundle_size": 1000,
     "synteny_plot_1_vs_all": false,
     "synteny_color_by_contig": true,
-    "synteny_xref_assemblies": "/workspace/assemblyqc/testdata/default/xrefsheet.csv",
+    "synteny_plotsr_seq_label": "Chr",
+    "synteny_plotsr_assembly_order": "gddh13_v1p1 m9_v1 m9_v1_h1 m9_v1_h2",
     "outdir": "./results",
     "email": null
 }

From 699e52c7d7636d159767162cd62b20eaf3e2919d Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 16:13:37 +1200
Subject: [PATCH 60/76] Updated docs

---
 docs/usage.md          | 2 +-
 tests/stub/params.json | 2 ++
 2 files changed, 3 insertions(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index a33a2c88..5c4b57ac 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -4,7 +4,7 @@
 
 You will need to create an assemblysheet with information about the assemblies you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with at least two columns, and a header row. An [example assemblysheet](../assets/assemblysheet.csv) has been provided with the pipeline. Its fields are:
 
-- `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report
+- `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report. The `tag` and `fasta` file name should not be same, such as `tag.fasta`. This can create file name collisions in the pipeline or result in file overwrite. It is also a good-practice to make all the input files read-only.
 - `fasta:` FASTA file
 - `gff3 [Optional]:` GFF3 annotation file if available
 - `monoploid_ids [Optional]:` A txt file listing the sequence IDs used to calculate LAI in monoploid mode if necessary. If the intent is to run LAI against all the sequences in an assembly, this file can be skipped for that assembly.
diff --git a/tests/stub/params.json b/tests/stub/params.json
index d3ea8333..6e303ac4 100644
--- a/tests/stub/params.json
+++ b/tests/stub/params.json
@@ -19,6 +19,8 @@
     "hic_skip_fastp": true,
     "hic_skip_fastqc": false,
     "synteny_skip": false,
+    "synteny_mummer_skip": false,
+    "synteny_plotsr_skip": false,
     "synteny_xref_assemblies": "assets/xrefsheet.csv",
     "merqury_skip": false,
     "max_cpus": 2,

From 03a23eac450659c78d27257c86343b1d030e58c5 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 17:06:07 +1200
Subject: [PATCH 61/76] Added assembly tag to synteny warning message

---
 CHANGELOG.md            | 1 +
 pfr/params.json         | 2 +-
 workflows/assemblyqc.nf | 2 +-
 3 files changed, 3 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 154aeeaf..2ef607a4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -36,6 +36,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 7. Fixed java memory overflow issues in `RUNASSEMBLYVISUALIZER`
 8. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]` [#83](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/83)
 9. Improved input assembly documentation [#86](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/86)
+10. Added assembly tag to synteny warning message regarding missing `synteny_labels` file
 
 ### `Dependencies`
 
diff --git a/pfr/params.json b/pfr/params.json
index cae8f7f9..3c10bfea 100644
--- a/pfr/params.json
+++ b/pfr/params.json
@@ -9,7 +9,7 @@
     "ncbi_fcs_gx_db_path": "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24",
     "contamination_stops_pipeline": false,
     "busco_skip": false,
-    "busco_mode": "geno",
+    "busco_mode": "genome",
     "busco_lineage_datasets": "embryophyta_odb10 eudicots_odb10",
     "busco_download_path": "/workspace/ComparativeDataSources/BUSCO/assemblyqc",
     "tidk_skip": false,
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 61b873d2..17e0c77f 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -105,7 +105,7 @@ workflow ASSEMBLYQC {
                                                     ? null
                                                     : log.warn("A synteny_labels file must be provided" +
                                                     " in the input assembly sheet when running synteny analysis." +
-                                                    " Synteny analysis is skipped!")
+                                                    " Synteny analysis is skipped for $tag")
                                                 )
                                             }
 

From 658d10e048f6558e57641b2496048bbf9e14e6d9 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 19:38:01 +1200
Subject: [PATCH 62/76] Updated docs

---
 README.md        | 28 +++++++++++++++-------------
 docs/output.md   | 10 +++++++---
 version_check.sh |  5 +++++
 3 files changed, 27 insertions(+), 16 deletions(-)

diff --git a/README.md b/README.md
index c279d69e..79af8cb4 100644
--- a/README.md
+++ b/README.md
@@ -112,22 +112,22 @@ sbatch ./pfr_assemblyqc
 
 ## Credits
 
-plant-food-research-open/assemblyqc was originally written by Usman Rashid and Ken Smith. Ross Crowhurst, Chen Wu and Marcus Davy generously contributed their QC scripts.
+plant-food-research-open/assemblyqc was originally written by Usman Rashid ([@gallvp](https://github.com/gallvp)) and Ken Smith ([@hzlnutspread](https://github.com/hzlnutspread)).
+
+Ross Crowhurst ([@rosscrowhurst](https://github.com/rosscrowhurst)), Chen Wu ([@christinawu2008](https://github.com/christinawu2008)) and Marcus Davy ([@mdavy86](https://github.com/mdavy86)) generously contributed their QC scripts.
+
+Mahesh Binzer-Panchal ([@mahesh-panchal](https://github.com/mahesh-panchal)) helped port the pipeline modules and sub-workflows to [nf-core](https://nf-co.re) schema.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
-- Cecilia Deng [@CeciliaDeng](https://github.com/CeciliaDeng)
-- Chen Wu [@christinawu2008](https://github.com/christinawu2008)
-- Ignacio Carvajal [@ignacio3437](https://github.com/ignacio3437)
-- Jason Shiller [@jasonshiller](https://github.com/jasonshiller)
-- Mahesh Binzer-Panchal [@mahesh-panchal](https://github.com/mahesh-panchal)
-- Marcus Davy [@mdavy86](https://github.com/mdavy86)
-- Ross Crowhurst [@rosscrowhurst](https://github.com/rosscrowhurst)
-- Sarah Bailey [@SarahBailey1998](https://github.com/SarahBailey1998)
-- Susan Thomson [@cflsjt](https://github.com/cflsjt)
-- Ting-Hsuan Chen [@ting-hsuan-chen](https://github.com/ting-hsuan-chen)
+- [Cecilia Deng](https://github.com/CeciliaDeng)
+- [Ignacio Carvajal](https://github.com/ignacio3437)
+- [Jason Shiller](https://github.com/jasonshiller)
+- [Sarah Bailey](https://github.com/SarahBailey1998)
+- [Susan Thomson](https://github.com/cflsjt)
+- [Ting-Hsuan Chen](https://github.com/ting-hsuan-chen)
 
-The pipeline uses nf-core modules contributed by following authors.
+The pipeline uses nf-core modules contributed by following authors:
 
 <a href="https://github.com/gallvp"><img src="https://github.com/gallvp.png" width="50" height="50"></a>
 <a href="https://github.com/drpatelh"><img src="https://github.com/drpatelh.png" width="50" height="50"></a>
@@ -164,7 +164,9 @@ If you would like to contribute to this pipeline, please see the [contributing g
 
 ## Citations
 
-If you use plant-food-research-open/assemblyqc for your analysis, please cite it using the following doi: [10.5281/zenodo.10647870](https://doi.org/10.5281/zenodo.10647870)
+If you use plant-food-research-open/assemblyqc for your analysis, please cite it as:
+
+> Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (2.0.0-rc.1). Zenodo. https://doi.org/10.5281/zenodo.10647870
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
diff --git a/docs/output.md b/docs/output.md
index ff7a724f..3cb7c048 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -205,9 +205,13 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 
 [Circos](https://circos.ca) and linear synteny plots are created from genome-wide alignments performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl).
 
-<div align="center"><img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="50%"><hr><em>AssemblyQC - Circos synteny plot</em></div>
-<div align="center"><img src="images/synteny_linear.png" alt="AssemblyQC - Dotplot synteny plot" width="50%"><hr><em>AssemblyQC - Dotplot synteny plot</em></div>
-<div align="center"><img src="images/plotsr.png" alt="AssemblyQC - Plotsr synteny plot" width="50%"><hr><em>AssemblyQC - Plotsr synteny plot</em></div>
+<div align="center">
+<img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="46%">
+<img src="images/plotsr.png" alt="AssemblyQC - Plotsr synteny plot" width="41.8%">
+<img src="images/synteny_linear.png" alt="AssemblyQC - Dotplot synteny plot" width="50%">
+<hr>
+<em>AssemblyQC - Synteny plots</em>
+</div>
 
 ### Merqury
 
diff --git a/version_check.sh b/version_check.sh
index 23cf619c..ca34d57e 100755
--- a/version_check.sh
+++ b/version_check.sh
@@ -12,3 +12,8 @@ fi
 
 head -10 CHANGELOG.md | grep "## v$config_version - " >/dev/null \
     || (echo 'Failed to match CHANGELOG version'; exit 1)
+
+# Check README version
+
+grep "assembly quality ($config_version)" README.md >/dev/null \
+    || (echo 'Failed to match README version'; exit 1)

From 18a8fb20791bc48831645acd96b04ab8fd627b63 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 28 May 2024 21:57:29 +1200
Subject: [PATCH 63/76] Updated flowchart

---
 README.md | 93 ++++++++++++++++++++++++++++++-------------------------
 1 file changed, 50 insertions(+), 43 deletions(-)

diff --git a/README.md b/README.md
index 79af8cb4..a9006228 100644
--- a/README.md
+++ b/README.md
@@ -16,55 +16,62 @@
 
 ```mermaid
 %%{init: {
-  "themeVariables": {
-    "fontSize": "52px"
+    'theme': 'base',
+    'themeVariables': {
+    'fontSize': '52px",
+    'primaryColor': '#9A6421',
+    'primaryTextColor': '#ffffff',
+    'primaryBorderColor': '#9A6421',
+    'lineColor': '#B180A8',
+    'secondaryColor': '#455C58',
+    'tertiaryColor': '#ffffff'
   }
 }}%%
 flowchart LR
-  forEachTag(For each\nAssembly) --> VALIDATE_FORMAT[VALIDATE FORMAT]
-
-  VALIDATE_FORMAT --> ncbiFCS[NCBI FCS\nADAPTOR]
-  ncbiFCS --> Check{Check}
-
-  VALIDATE_FORMAT --> ncbiGX[NCBI FCS GX]
-  ncbiGX --> Check
-  Check --> |Clean|Run(Run)
-
-  Check --> |Contamination|Skip(Skip All)
-  Skip --> REPORT
-
-  VALIDATE_FORMAT --> GFF_STATS[GENOMETOOLS GT STAT]
-
-  Run --> ASS_STATS[ASSEMBLATHON STATS]
-  Run --> BUSCO
-  Run --> TIDK
-  Run --> LAI
-  Run --> KRAKEN2
-  Run --> HIC_CONTACT_MAP[HIC CONTACT MAP]
-  Run --> MUMMER
-  Run --> MINIMAP2
-  Run --> MERQURY
-
-  MUMMER --> CIRCOS
-  MUMMER --> DOTPLOT
-
-  MINIMAP2 --> PLOTSR
-
-  ASS_STATS --> REPORT
-  GFF_STATS --> REPORT
-  BUSCO --> REPORT
-  TIDK --> REPORT
-  LAI --> REPORT
-  KRAKEN2 --> REPORT
-  HIC_CONTACT_MAP --> REPORT
-  CIRCOS --> REPORT
-  DOTPLOT --> REPORT
-  PLOTSR --> REPORT
-  MERQURY --> REPORT
+  forEachTag(Assembly) ==> VALIDATE_FORMAT[VALIDATE FORMAT]
+
+  VALIDATE_FORMAT ==> ncbiFCS[NCBI FCS\nADAPTOR]
+  ncbiFCS ==> Check{Check}
+
+  VALIDATE_FORMAT ==> ncbiGX[NCBI FCS GX]
+  ncbiGX ==> Check
+  Check ==> |Clean|Run(Run)
+
+  Check ==> |Contamination|Skip(Skip All)
+  Skip ==> REPORT
+
+  VALIDATE_FORMAT ==> GFF_STATS[GENOMETOOLS GT STAT]
+
+  Run ==> ASS_STATS[ASSEMBLATHON STATS]
+  Run ==> BUSCO
+  Run ==> TIDK
+  Run ==> LAI
+  Run ==> KRAKEN2
+  Run ==> HIC_CONTACT_MAP[HIC CONTACT MAP]
+  Run ==> MUMMER
+  Run ==> MINIMAP2
+  Run ==> MERQURY
+
+  MUMMER ==> CIRCOS
+  MUMMER ==> DOTPLOT
+
+  MINIMAP2 ==> PLOTSR
+
+  ASS_STATS ==> REPORT
+  GFF_STATS ==> REPORT
+  BUSCO ==> REPORT
+  TIDK ==> REPORT
+  LAI ==> REPORT
+  KRAKEN2 ==> REPORT
+  HIC_CONTACT_MAP ==> REPORT
+  CIRCOS ==> REPORT
+  DOTPLOT ==> REPORT
+  PLOTSR ==> REPORT
+  MERQURY ==> REPORT
 ```
 
 - [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator) + [SEQKIT RMDUP](https://github.com/shenwei356/seqkit): FASTA validation
-- [GenomeTools GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation
+- [GENOMETOOLS GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation
 - [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics
 - [GENOMETOOLS GT STAT](https://genometools.org/tools/gt_stat.html): Annotation statistics
 - [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail

From 543625abe8be9c569eb84d489865a7304b55c960 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 29 May 2024 11:31:33 +1200
Subject: [PATCH 64/76] Fixed a bug in file order for plotsr

---
 conf/modules.config                 |   3 +++
 docs/images/plotsr.png              | Bin 52708 -> 116992 bytes
 docs/usage.md                       |   5 ++++-
 subworkflows/local/fasta_synteny.nf |  30 +++++++++++++++++++---------
 4 files changed, 28 insertions(+), 10 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 1b6ee2a1..42bd196d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -167,6 +167,9 @@ process {
     }
 
     withName: '.*:FASTA_SYNTENY:PLOTSR' {
+
+        ext.args = '-d 600'
+
         publishDir = [
             path: { "${params.outdir}/synteny/plotsr" },
             mode: params.publish_dir_mode,
diff --git a/docs/images/plotsr.png b/docs/images/plotsr.png
index ed35e6f85b36f5a0f3bc408b47d70a688818cc23..ec160b0d9d3073444b695bd36e27402d826cbe2a 100644
GIT binary patch
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diff --git a/docs/usage.md b/docs/usage.md
index 5c4b57ac..b211bf27 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -7,7 +7,7 @@ You will need to create an assemblysheet with information about the assemblies y
 - `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report. The `tag` and `fasta` file name should not be same, such as `tag.fasta`. This can create file name collisions in the pipeline or result in file overwrite. It is also a good-practice to make all the input files read-only.
 - `fasta:` FASTA file
 - `gff3 [Optional]:` GFF3 annotation file if available
-- `monoploid_ids [Optional]:` A txt file listing the sequence IDs used to calculate LAI in monoploid mode if necessary. If the intent is to run LAI against all the sequences in an assembly, this file can be skipped for that assembly.
+- `monoploid_ids [Optional]:` A txt file listing the sequence IDs used to calculate LAI in monoploid mode if necessary. If the intent is to run LAI against all the sequences in an assembly, this file can be skipped for that assembly. Soft masked regions are ignored when calculating LAI. The pipeline may fail if all the LTRs are already soft masked.
 - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence IDs (first column) and their labels for the synteny plots (second column) when performing synteny analysis. If a sequence ID is missing from this file, the corresponding sequence is excluded from the analysis. If `synteny_labels` is not provided for an assembly, that assembly is excluded from the analysis.
 
 See the [Merqury](#merqury-k-mer-analysis) section For description of assemblysheet columns related to k-mer analysis with Merqury.
@@ -72,6 +72,9 @@ This section provides additional information for parameters. It does not list al
 
 - `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom. If this parameter is `null`, the assemblies are ordered alphabetically. All assemblies from `input` and `synteny_xref_assemblies` are included by default. If an assembly is missing from this list, that assembly is excluded from the analysis.
 
+> [!NOTE]
+> PLOTSR performs a sequence-wise (preferably chromosome-wise) synteny analysis. The order of the sequences for each assembly is inferred from its `synteny_labels` file.
+
 ### Merqury K-mer analysis
 
 Additional assemblysheet columns:
diff --git a/subworkflows/local/fasta_synteny.nf b/subworkflows/local/fasta_synteny.nf
index 9f8afaa0..0ca879f5 100644
--- a/subworkflows/local/fasta_synteny.nf
+++ b/subworkflows/local/fasta_synteny.nf
@@ -272,11 +272,14 @@ workflow FASTA_SYNTENY {
                                         | map { [ it ] }
                                         | collect
                                         | map { list ->
-                                            if ( plotsr_assembly_order == null) {
+                                            if ( plotsr_assembly_order == null ) {
                                                 return list.sort(false) { it[0].id.toUpperCase() }
                                             }
 
                                             def order   = plotsr_assembly_order.tokenize(' ')
+
+                                            if ( order.size() != order.unique().size() ) error "Tags listed by synteny_plotsr_assembly_order should all be unique: $order"
+
                                             def tags    = list.collect { it[0].id }
 
                                             def ordered_list = []
@@ -333,23 +336,32 @@ workflow FASTA_SYNTENY {
                                             [
                                                 [ id: 'plotsr' ],
                                                 syri,
-                                                [ rfa, tfa ] // Order matters, see https://github.com/schneebergerlab/plotsr/issues/70
+                                                [ tfa, rfa]
                                             ]
                                         }
                                         | groupTuple
                                         | map { meta, syri, fastas ->
+                                            def fasta_list          = fastas.flatten()
+                                            def syri_tags           = syri.collect { it.name.replace('syri.out', '').tokenize('.on.') }.flatten().unique()
+                                            def proposed_order      = plotsr_assembly_order ? plotsr_assembly_order.tokenize(' ') : syri_tags.sort(false)
 
-                                            def unique_fa = []
+                                            def available_tags      = []
+                                            proposed_order.each { tag -> if ( tag in syri_tags ) available_tags << tag }
 
-                                            fastas.flatten().each { fasta ->
-                                                if ( ! ( fasta in unique_fa ) ) { unique_fa << fasta }
-                                            }
+                                            def ordered_fa          = []
+                                            available_tags.each { tag -> ordered_fa << ( fasta_list.find { it.baseName == "${tag}.plotsr" } ) }
+
+                                            def ordered_syri_tags   = []
+                                            available_tags.eachWithIndex { tag, index -> if ( index > 0 ) { ordered_syri_tags << "${tag}.on.${available_tags[index-1]}" } }
+
+                                            def ordered_syri        = []
+                                            ordered_syri_tags.each { tag -> ordered_syri << ( syri.find { it.baseName == "${tag}syri" } ) }
 
                                             [
                                                 meta,
-                                                syri,
-                                                unique_fa,
-                                                "#file\tname\n" + unique_fa.collect { it.baseName.replace('.plotsr', '') }.join('\n')
+                                                ordered_syri,
+                                                ordered_fa,
+                                                "#file\tname\n" + ordered_fa.collect { it.baseName.replace('.plotsr', '') }.join('\n')
                                             ]
                                         }
 

From 3ff806fb0357b52b8c8683403f551dd26eb7eda2 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Wed, 29 May 2024 11:48:00 +1200
Subject: [PATCH 65/76] Set merqury_skip to false in pfr/params

---
 CHANGELOG.md    | 2 +-
 CITATION.cff    | 2 +-
 README.md       | 2 +-
 nextflow.config | 2 +-
 pfr/params.json | 1 +
 5 files changed, 5 insertions(+), 4 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 2ef607a4..329f5a69 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0-rc.1 - [28-May-2024]
+## v2.0.0 - [29-May-2024]
 
 ### `Added`
 
diff --git a/CITATION.cff b/CITATION.cff
index f3d7d693..23125eae 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -21,7 +21,7 @@ authors:
   - family-names: "Deng"
     given-names: "Cecilia"
 title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality"
-version: 2.0.0-rc.1
+version: 2.0.0
 date-released: 2024-02-12
 url: "https://github.com/Plant-Food-Research-Open/assembly_qc"
 doi: 10.5281/zenodo.10647870
diff --git a/README.md b/README.md
index a9006228..74f56c0d 100644
--- a/README.md
+++ b/README.md
@@ -173,7 +173,7 @@ If you would like to contribute to this pipeline, please see the [contributing g
 
 If you use plant-food-research-open/assemblyqc for your analysis, please cite it as:
 
-> Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (2.0.0-rc.1). Zenodo. https://doi.org/10.5281/zenodo.10647870
+> Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (2.0.0). Zenodo. https://doi.org/10.5281/zenodo.10647870
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
diff --git a/nextflow.config b/nextflow.config
index f494a1c8..1f445720 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -283,7 +283,7 @@ manifest {
     description     = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '2.0.0-rc.1'
+    version         = '2.0.0'
     doi             = 'https://doi.org/10.5281/zenodo.10647870'
 }
 
diff --git a/pfr/params.json b/pfr/params.json
index 3c10bfea..8453604b 100644
--- a/pfr/params.json
+++ b/pfr/params.json
@@ -36,6 +36,7 @@
     "synteny_color_by_contig": true,
     "synteny_plotsr_seq_label": "Chr",
     "synteny_plotsr_assembly_order": "gddh13_v1p1 m9_v1 m9_v1_h1 m9_v1_h2",
+    "merqury_skip": false,
     "outdir": "./results",
     "email": null
 }

From f590cc2870e3b862fea81a96ceb6b86f9dba8485 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 30 May 2024 19:20:44 +1200
Subject: [PATCH 66/76] Increased time limit for MERQURY_HAPMERS

---
 .../{synteny_linear.html => synteny_dotplot.html}              | 0
 conf/base.config                                               | 3 +++
 2 files changed, 3 insertions(+)
 rename bin/report_modules/templates/synteny_dotplot/{synteny_linear.html => synteny_dotplot.html} (100%)

diff --git a/bin/report_modules/templates/synteny_dotplot/synteny_linear.html b/bin/report_modules/templates/synteny_dotplot/synteny_dotplot.html
similarity index 100%
rename from bin/report_modules/templates/synteny_dotplot/synteny_linear.html
rename to bin/report_modules/templates/synteny_dotplot/synteny_dotplot.html
diff --git a/conf/base.config b/conf/base.config
index 1f1878db..87a5d686 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -72,6 +72,9 @@ process {
     withName:DNADIFF {
         time   = { check_max( 7.day  * task.attempt, 'time'    ) }
     }
+    withName:MERQURY_HAPMERS {
+        time   = { check_max( 20.h  * task.attempt, 'time'    ) }
+    }
     withName:CREATEREPORT {
         cache   = false
     }

From 04bfcd13c9e439522312bb1769a5e8462c25adc1 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 30 May 2024 19:26:06 +1200
Subject: [PATCH 67/76] Fixed merqury version reference

---
 bin/report_modules/templates/merqury/merqury.html | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/bin/report_modules/templates/merqury/merqury.html b/bin/report_modules/templates/merqury/merqury.html
index 0090e222..528fb47f 100644
--- a/bin/report_modules/templates/merqury/merqury.html
+++ b/bin/report_modules/templates/merqury/merqury.html
@@ -10,7 +10,7 @@
         target="_blank">10.1186/s13059-020-02134-9</a>
     </p>
     <p class="section-para">
-      <b>Version: {{ all_stats_dicts['VERSIONS']['MERQURY']['merqury'] }}</b>
+      <b>Version: {{ all_stats_dicts['VERSIONS']['MERQURY_MERQURY']['merqury'] }}</b>
     </p>
   </div>
   {% include 'merqury/dropdown.html' %} {% include 'merqury/report_contents.html' %}

From a95d3f05b3e038a232ccfcfcda615b0b26e8bb71 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 30 May 2024 19:52:37 +1200
Subject: [PATCH 68/76] Disabled legend on dotplot

---
 CHANGELOG.md         | 2 +-
 bin/linearsynteny.py | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 329f5a69..5ac93331 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0 - [29-May-2024]
+## v2.0.0 - [30-May-2024]
 
 ### `Added`
 
diff --git a/bin/linearsynteny.py b/bin/linearsynteny.py
index 1bc2cd46..6bb44eb9 100755
--- a/bin/linearsynteny.py
+++ b/bin/linearsynteny.py
@@ -126,7 +126,7 @@ def format_figure(
         ),
         paper_bgcolor="rgba(0,0,0,0)",
         plot_bgcolor="rgba(0,0,0,0)",
-        showlegend=True,
+        showlegend=False,
     )
 
     fig.update_xaxes(

From c9a1a1c6d668123c63bdf343ba2a69b35bbf3e5f Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Thu, 30 May 2024 20:21:02 +1200
Subject: [PATCH 69/76] Updated docs

---
 docs/images/dotplot.png        | Bin 0 -> 218630 bytes
 docs/images/hapmers_blob.png   | Bin 0 -> 238184 bytes
 docs/images/synteny_linear.png | Bin 108201 -> 0 bytes
 docs/output.md                 |   9 +++++++--
 4 files changed, 7 insertions(+), 2 deletions(-)
 create mode 100644 docs/images/dotplot.png
 create mode 100644 docs/images/hapmers_blob.png
 delete mode 100644 docs/images/synteny_linear.png

diff --git a/docs/images/dotplot.png b/docs/images/dotplot.png
new file mode 100644
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AZ2$lO

diff --git a/docs/output.md b/docs/output.md
index 3cb7c048..b1cbf180 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -208,7 +208,7 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 <div align="center">
 <img src="images/synteny_circos.png" alt="AssemblyQC - Circos synteny plot" width="46%">
 <img src="images/plotsr.png" alt="AssemblyQC - Plotsr synteny plot" width="41.8%">
-<img src="images/synteny_linear.png" alt="AssemblyQC - Dotplot synteny plot" width="50%">
+<img src="images/dotplot.png" alt="AssemblyQC - Dotplot synteny plot" width="50%">
 <hr>
 <em>AssemblyQC - Synteny plots</em>
 </div>
@@ -228,7 +228,12 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe
 
 [MERQURY](https://github.com/marbl/merqury) is used for the k-mer analysis.
 
-<div align="center"><img src="images/spectra_cn.png" alt="AssemblyQC - Spectra-cn plot" width="50%"><hr><em>AssemblyQC - Spectra-cn plot</em></div>
+<div align="center">
+<img src="images/spectra_cn.png" alt="AssemblyQC - Spectra-cn plot" width="49%">
+<img src="images/hapmers_blob.png" alt="AssemblyQC - Plotsr synteny plot" width="39.8%">
+<hr>
+<em>AssemblyQC - Merqury plots</em>
+</div>
 
 ### Pipeline information
 

From 1b713cf840e60bce0629757e6cdc5e353336eb0f Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 31 May 2024 11:57:34 +1200
Subject: [PATCH 70/76] Fixed headers in the merqury qv stats table

---
 CHANGELOG.md                                 | 2 +-
 bin/report_modules/parsers/merqury_parser.py | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5ac93331..7b5c703b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0 - [30-May-2024]
+## v2.0.0 - [31-May-2024]
 
 ### `Added`
 
diff --git a/bin/report_modules/parsers/merqury_parser.py b/bin/report_modules/parsers/merqury_parser.py
index 6ba0b8e7..fb584611 100644
--- a/bin/report_modules/parsers/merqury_parser.py
+++ b/bin/report_modules/parsers/merqury_parser.py
@@ -56,7 +56,7 @@ def parse_merqury_folder(folder_name="merqury_outputs"):
                 "qv_stats_table": qv_stats_table.to_dict("records"),
                 "qv_stats_table_html": tabulate(
                     qv_stats_table,
-                    headers=["Assembly", "No Support", "Total", "Error %", "QV"],
+                    headers=["Assembly", "No Support", "Total", "QV", "Error %"],
                     tablefmt="html",
                     numalign="left",
                     showindex=False,

From 3548df4d7f742f42894f587405e2f446ea6e8b78 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Fri, 31 May 2024 13:46:04 +1200
Subject: [PATCH 71/76] Removed unnecessary ncbi_fcs_gx_tax_id

---
 pfr_assemblyqc | 1 -
 1 file changed, 1 deletion(-)

diff --git a/pfr_assemblyqc b/pfr_assemblyqc
index f92ae0d2..8f40d31d 100644
--- a/pfr_assemblyqc
+++ b/pfr_assemblyqc
@@ -38,7 +38,6 @@ if [ $full_test_flag -eq 1 ]; then
         -c pfr/profile.config \
         -profile pfr,apptainer,test_full \
         --ncbi_fcs_gx_skip false \
-        --ncbi_fcs_gx_tax_id 35717 \
         --ncbi_fcs_gx_db_path "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" \
         --kraken2_skip false \
         --kraken2_db_path "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" \

From 019fb3793a2f7521af9c1ec2cb06531bee6b2d03 Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 3 Jun 2024 12:09:56 +1200
Subject: [PATCH 72/76] Updated fasta_ltrretriever_lai

---
 CHANGELOG.md                                  |   2 +-
 modules.json                                  |   4 +-
 .../custom/shortenfastaids/environment.yml    |   2 +-
 modules/pfr/custom/shortenfastaids/main.nf    |   3 +-
 .../templates/shorten_fasta_ids.py            |   2 +
 .../custom/shortenfastaids/tests/main.nf.test |   6 +-
 .../shortenfastaids/tests/main.nf.test.snap   |  36 ++++--
 .../pfr/fasta_ltrretriever_lai/main.nf        | 122 ++++++++++++------
 .../fasta_ltrretriever_lai/tests/main.nf.test |  82 ++----------
 .../tests/main.nf.test.snap                   |  96 ++++++++++++++
 workflows/assemblyqc.nf                       |  10 +-
 11 files changed, 232 insertions(+), 133 deletions(-)
 create mode 100644 subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7b5c703b..a7393fc5 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0 - [31-May-2024]
+## v2.0.0 - [03-June-2024]
 
 ### `Added`
 
diff --git a/modules.json b/modules.json
index 894d0ade..51abb62d 100644
--- a/modules.json
+++ b/modules.json
@@ -42,7 +42,7 @@
                     },
                     "custom/shortenfastaids": {
                         "branch": "main",
-                        "git_sha": "da7a50af5eaac9601e23f33e00bf24e61f9c27ab",
+                        "git_sha": "e226301053a2b0f9a6af514b454b3ae7c91daa80",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "gffread": {
@@ -121,7 +121,7 @@
                     },
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
-                        "git_sha": "8088445a270d324cb06e085f18790839c9684754",
+                        "git_sha": "d57f5fb09ab3edca3d4e16dd41beb684a7192d04",
                         "installed_by": ["subworkflows"]
                     },
                     "fastq_bwa_mem_samblaster": {
diff --git a/modules/pfr/custom/shortenfastaids/environment.yml b/modules/pfr/custom/shortenfastaids/environment.yml
index e80fa7cf..a64758c6 100644
--- a/modules/pfr/custom/shortenfastaids/environment.yml
+++ b/modules/pfr/custom/shortenfastaids/environment.yml
@@ -8,4 +8,4 @@ channels:
 
 dependencies:
   - biopython==1.75
-  - python=3.8
+  - python==3.8.13
diff --git a/modules/pfr/custom/shortenfastaids/main.nf b/modules/pfr/custom/shortenfastaids/main.nf
index 92762ef6..cef0796c 100644
--- a/modules/pfr/custom/shortenfastaids/main.nf
+++ b/modules/pfr/custom/shortenfastaids/main.nf
@@ -12,7 +12,7 @@ process CUSTOM_SHORTENFASTAIDS {
 
     output:
     tuple val(meta), path("*.short.ids.fasta")  , emit: short_ids_fasta , optional: true
-    tuple val(meta), path("*.short.ids.tsv")    , emit: short_ids_tsv   , optional: true
+    tuple val(meta), path("*.short.ids.tsv")    , emit: short_ids_tsv
     path "versions.yml"                         , emit: versions
 
     when:
@@ -25,6 +25,7 @@ process CUSTOM_SHORTENFASTAIDS {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
+    touch "${meta.id}.short.ids.tsv"
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         python: \$(python --version | cut -d' ' -f2)
diff --git a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py
index 67d6f565..0ce43903 100755
--- a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py
+++ b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py
@@ -165,6 +165,8 @@ def fail_if_new_ids_not_valid(ids):
 
     if not do_ids_need_to_change(input_ids_and_descriptions):
         print("IDs have acceptable length and character. No change required.")
+        with open(f"{output_files_prefix}.short.ids.tsv", "w") as f:
+            f.write("IDs have acceptable length and character. No change required.")
         exit(0)
 
     new_ids = shorten_ids(
diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test b/modules/pfr/custom/shortenfastaids/tests/main.nf.test
index 4c6fbe58..c87e8ebf 100644
--- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test
+++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test
@@ -5,7 +5,7 @@ nextflow_process {
     process "CUSTOM_SHORTENFASTAIDS"
 
     tag "modules"
-    tag "modules_nfcore"
+    tag "modules_pfr"
     tag "custom"
     tag "custom/shortenfastaids"
 
@@ -25,9 +25,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert process.out.short_ids_fasta == [] },
-                { assert process.out.short_ids_tsv == [] }
+                { assert snapshot(process.out).match() }
             )
         }
 
diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
index 89b9483a..49fa2afb 100644
--- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
+++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
@@ -55,7 +55,12 @@
                     
                 ],
                 "1": [
-                    
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.short.ids.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "2": [
                     "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
@@ -64,7 +69,12 @@
                     
                 ],
                 "short_ids_tsv": [
-                    
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.short.ids.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "versions": [
                     "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
@@ -73,9 +83,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.1"
         },
-        "timestamp": "2024-05-10T15:27:57.244358"
+        "timestamp": "2024-06-02T20:54:36.815875"
     },
     "homo_sapiens-genome_fasta-no_change": {
         "content": [
@@ -84,7 +94,12 @@
                     
                 ],
                 "1": [
-                    
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.short.ids.tsv:md5,642382addc4beba37088b1ebe09d38cf"
+                    ]
                 ],
                 "2": [
                     "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
@@ -93,7 +108,12 @@
                     
                 ],
                 "short_ids_tsv": [
-                    
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.short.ids.tsv:md5,642382addc4beba37088b1ebe09d38cf"
+                    ]
                 ],
                 "versions": [
                     "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba"
@@ -102,9 +122,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.1"
         },
-        "timestamp": "2023-12-07T13:32:54.220188"
+        "timestamp": "2024-06-02T20:54:17.945233"
     },
     "homo_sapiens-genome2_fasta-length_change": {
         "content": [
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
index 5c392795..09a293fc 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
@@ -10,39 +10,65 @@ workflow FASTA_LTRRETRIEVER_LAI {
 
     take:
     ch_fasta                        // channel: [ val(meta), fasta ]
-    ch_monoploid_seqs               // channel: [ val(meta), txt ]; Optional: Set to [] if not needed
-                                    // val(meta) from ch_fasta and ch_monoploid_seqs are only required
-                                    // to have the same `id`
+    ch_monoploid_seqs               // channel: [ val(meta2), txt ]; Optional: Set to [] if not needed
+                                    // val(meta) from ch_fasta and val(meta2) from ch_monoploid_seqs are
+                                    // only required to have the same `id`
     skip_lai                        // val(true|false)
 
     main:
     ch_versions                     = Channel.empty()
 
+    // Prapre input channels
+    ch_monoploid_seqs_plain         = ch_monoploid_seqs
+                                    ?: Channel.empty()
+                                    | filter { meta2, seqs -> seqs }
+                                    // Cater to channel: [ meta2, [] ]
+                                    | map { meta2, seqs -> [ meta2.id, seqs ] }
+
     // MOUDLE: CUSTOM_SHORTENFASTAIDS
     CUSTOM_SHORTENFASTAIDS ( ch_fasta )
 
-    ch_short_ids_fasta              = ch_fasta
-                                    | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true)
-                                    | map { meta, fasta, short_ids_fasta ->
-                                        if ( fasta ) { [ meta, short_ids_fasta ?: fasta ] }
+    ch_short_ids_tsv                = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv
+    ch_shortenfastaids_branch       = ch_short_ids_tsv
+                                    | branch { meta, tsv ->
+                                        change: ! tsv.text.contains('IDs have acceptable length and character')
+                                        nonchange: tsv.text.contains('IDs have acceptable length and character')
                                     }
 
-    ch_short_ids_tsv                = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv
-    ch_short_monoploid_seqs         = ch_short_ids_tsv
+    ch_short_ids_fasta              = ch_shortenfastaids_branch.nonchange
                                     | join(
-                                        ch_monoploid_seqs ?: Channel.empty()
+                                        ch_fasta
+                                    )
+                                    | map { meta, tsv, fasta -> [ meta, fasta ] }
+                                    | mix(
+                                        ch_shortenfastaids_branch.change
+                                        | join(
+                                            CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta
+                                        )
+                                        | map { meta, tsv, fasta -> [ meta, fasta ] }
                                     )
-                                    | filter { meta, tsv, seqs -> seqs } // Cater to channel: [ meta, tsv, [] ]
-                                    | map { meta, short_ids_tsv, monoploid_seqs ->
-                                        map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs)
+
+    ch_versions                     = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first())
+
+    // collectFile: Map monoploid seqs to short IDs
+    ch_short_monoploid_seqs         = ch_short_ids_tsv
+                                    | map { meta, tsv -> [ meta.id, tsv ] }
+                                    | join(ch_monoploid_seqs_plain)
+                                    | map { id, tsv, seqs ->
+                                        map_monoploid_seqs_to_new_ids(id, tsv, seqs)
                                     }
                                     | collectFile(newLine:true)
                                     | map { seqs ->
                                         def id = seqs.name.split('.mapped.monoploid.seqs.txt')[0]
 
-                                        [ [ id: id ], seqs ]
+                                        [ id, seqs ]
                                     }
-    ch_versions                     = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first())
+                                    | join(
+                                        ch_short_ids_tsv
+                                        | map { meta, tsv -> [ meta.id, meta, tsv ] }
+                                    )
+                                    | map { id, seqs, meta, tsv -> [ meta, seqs ] }
+
 
     // MODULE: LTRHARVEST
     LTRHARVEST ( ch_short_ids_fasta )
@@ -78,34 +104,42 @@ workflow FASTA_LTRRETRIEVER_LAI {
     )
 
     ch_pass_list                    = LTRRETRIEVER_LTRRETRIEVER.out.pass_list
-    ch_ltrlib                       = LTRRETRIEVER_LTRRETRIEVER.out.ltrlib
     ch_annotation_out               = LTRRETRIEVER_LTRRETRIEVER.out.annotation_out
+    ch_pass_out                     = ch_pass_list.join(ch_annotation_out)
     ch_annotation_gff               = LTRRETRIEVER_LTRRETRIEVER.out.annotation_gff
+    ch_ltrlib                       = LTRRETRIEVER_LTRRETRIEVER.out.ltrlib
     ch_versions                     = ch_versions.mix(LTRRETRIEVER_LTRRETRIEVER.out.versions.first())
 
-    // MODULE: LAI
-    ch_lai_inputs                   = skip_lai
-                                    ? Channel.empty()
-                                    : ch_short_ids_fasta
-                                    | join(ch_pass_list)
-                                    | join(ch_annotation_out)
-                                    | map { meta, fasta, pass, out ->
-                                        [ meta.id, meta, fasta, pass, out ]
-                                    }
+    // MODULE: LTRRETRIEVER_LAI
+    ch_short_ids_fasta_mono         = ch_short_ids_fasta
                                     | join(
-                                        ch_short_monoploid_seqs
-                                        | map { meta, mono -> [ meta.id, mono ] },
+                                        ch_short_monoploid_seqs,
                                         by:0,
                                         remainder: true
                                     )
-                                    | map { id, meta, fasta, pass, out, mono ->
-                                        [ meta, fasta, pass, out, mono ?: [] ]
+                                    // Danger! This partial join can fail
+                                    | filter { meta, fasta, seqs -> fasta }
+                                    // This filter safeguards against fail on upstream
+                                    // process failure: https://github.com/nextflow-io/nextflow/issues/5043
+                                    // fasta may come from upstream processes
+                                    // seqs also comes from upstream processes, it is optional
+                                    // and may not be present for some of the combinations
+                                    | map { id, fasta, seqs -> [ id, fasta, seqs ?: [] ] }
+
+    ch_lai_inputs                   = skip_lai
+                                    ? Channel.empty()
+                                    : ch_short_ids_fasta_mono
+                                    | join(
+                                        ch_pass_out
+                                    )
+                                    | map { meta, fasta, seqs, pass, out ->
+                                        [ meta, fasta, pass, out, seqs ]
                                     }
     LTRRETRIEVER_LAI(
-        ch_lai_inputs.map { meta, fasta, pass, out, mono -> [ meta, fasta ] },
-        ch_lai_inputs.map { meta, fasta, pass, out, mono -> pass },
-        ch_lai_inputs.map { meta, fasta, pass, out, mono -> out },
-        ch_lai_inputs.map { meta, fasta, pass, out, mono -> mono }
+        ch_lai_inputs.map { meta, fasta, pass, out, seqs -> [ meta, fasta ] },
+        ch_lai_inputs.map { meta, fasta, pass, out, seqs -> pass },
+        ch_lai_inputs.map { meta, fasta, pass, out, seqs -> out },
+        ch_lai_inputs.map { meta, fasta, pass, out, seqs -> seqs }
     )
 
     ch_lai_log                      = LTRRETRIEVER_LAI.out.log
@@ -113,16 +147,20 @@ workflow FASTA_LTRRETRIEVER_LAI {
     ch_versions                     = ch_versions.mix(LTRRETRIEVER_LAI.out.versions.first())
 
     // MODULE: CUSTOM_RESTOREGFFIDS
-    ch_restorable_gff_tsv           = ch_annotation_gff.join(ch_short_ids_tsv)
+    ch_gff_tsv_branch               = ch_annotation_gff.join(ch_short_ids_tsv)
+                                    | branch { meta, gff, tsv ->
+                                        change: ! tsv.text.contains('IDs have acceptable length and character')
+                                        nochange: tsv.text.contains('IDs have acceptable length and character')
+                                    }
 
     CUSTOM_RESTOREGFFIDS (
-        ch_restorable_gff_tsv.map { meta, gff, tsv -> [ meta, gff ] },
-        ch_restorable_gff_tsv.map { meta, gff, tsv -> tsv }
+        ch_gff_tsv_branch.change.map { meta, gff, tsv -> [ meta, gff ] },
+        ch_gff_tsv_branch.change.map { meta, gff, tsv -> tsv }
     )
 
-    ch_restored_gff                 = ch_annotation_gff
-                                    | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true)
-                                    | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] }
+    ch_restored_gff                 = ch_gff_tsv_branch.nochange
+                                    | map { meta, gff, tsv -> [ meta, gff ] }
+                                    | mix(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3)
 
     ch_versions                     = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first())
 
@@ -135,12 +173,12 @@ workflow FASTA_LTRRETRIEVER_LAI {
 }
 
 
-def map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) {
+def map_monoploid_seqs_to_new_ids(id, short_ids_tsv, monoploid_seqs) {
 
     def short_ids_head = short_ids_tsv.text.split('\n')[0]
 
     if (short_ids_head == "IDs have acceptable length and character. No change required.") {
-        return [ "${meta.id}.mapped.monoploid.seqs.txt" ] + monoploid_seqs.text.split('\n')
+        return [ "${id}.mapped.monoploid.seqs.txt" ] + monoploid_seqs.text.split('\n')
     }
 
     def orig_to_new_ids = [:]
@@ -159,5 +197,5 @@ def map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) {
         mapped_ids.add(orig_to_new_ids[original_id])
     }
 
-    return [ "${meta.id}.mapped.monoploid.seqs.txt" ] + mapped_ids
+    return [ "${id}.mapped.monoploid.seqs.txt" ] + mapped_ids
 }
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
index 4eff67ca..192ccef6 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
@@ -6,7 +6,7 @@ nextflow_workflow {
     config "./nextflow.config"
 
     tag "subworkflows"
-    tag "subworkflows_nfcore"
+    tag "subworkflows_pfr"
     tag "subworkflows/fasta_ltrretriever_lai"
     tag "fasta_ltrretriever_lai"
     tag "modules/nf-core/gunzip"
@@ -52,7 +52,8 @@ nextflow_workflow {
                 { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') },
                 { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') },
                 { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 },
-                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }
+                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') },
+                { assert snapshot(workflow.out.versions).match("fasta_gz_versions") }
             )
         }
     }
@@ -87,39 +88,8 @@ nextflow_workflow {
                 { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') },
                 { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') },
                 { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 },
-                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }
-            )
-        }
-    }
-
-    test("actinidia_chinensis-genome_1_fasta_gz-with_empty_mono_channel") {
-
-        when {
-            workflow {
-                """
-                GUNZIP.out.gunzip.map { meta, fasta ->
-                    def fa          = new File('test.fa')
-                    fa.write(fasta.text.replaceAll('>chr1', '>chr_xxxxxxxxxxxxxxx_1'))
-
-                    [ meta, fa.toPath() ]
-                }
-                | set { ch_fa }
-
-                input[0]            = ch_fa
-                input[1]            = ch_fa.map { meta, fasta -> [ meta, [] ] }
-                input[2]            = false
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') },
-                { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') },
-                { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') },
-                { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 },
-                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }
+                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') },
+                { assert snapshot(workflow.out.versions).match("with_mono_versions") }
             )
         }
     }
@@ -151,43 +121,8 @@ nextflow_workflow {
                 { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') },
                 { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') },
                 { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 },
-                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }
-            )
-        }
-    }
-
-    test("actinidia_chinensis-genome_1_fasta_gz-without_lai") {
-
-        options '-stub'
-
-        when {
-            workflow {
-                """
-                GUNZIP.out.gunzip.map { meta, fasta ->
-                    def fa          = new File('test.fa')
-                    fa.write(fasta.text.replaceAll('>chr1', '>chr_xxxxxxxxxxxxxxx_1'))
-
-                    [ meta, fa.toPath() ]
-                }
-                | set { ch_fa }
-
-                def monoploid_seqs  = new File('test.mono.seq.txt')
-                monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1")
-
-                input[0]            = ch_fa
-                input[1]            = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] )
-                input[2]            = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert workflow.out.annotation_gff != null },
-                { assert workflow.out.ltrlib != null },
-                { assert workflow.out.lai_log == [] },
-                { assert workflow.out.lai_out == [] }
+                { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') },
+                { assert snapshot(workflow.out.versions).match("with_empty_mono_versions") }
             )
         }
     }
@@ -211,7 +146,8 @@ nextflow_workflow {
 
         then {
             assertAll(
-                { assert workflow.success }
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
             )
         }
     }
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap
new file mode 100644
index 00000000..4ce42ec1
--- /dev/null
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap
@@ -0,0 +1,96 @@
+{
+    "fasta_gz_versions": {
+        "content": [
+            [
+                "versions.yml:md5,05a74dacbcd6c5906faeca3206f6563f",
+                "versions.yml:md5,52a98b45c3cf73f9314dbe9b34eca9d8",
+                "versions.yml:md5,5d86cda262f5c21e43738433a2781ebc",
+                "versions.yml:md5,608d746d45680f025562c4be455db461",
+                "versions.yml:md5,8468404805dddec679524e75b4fa51e8",
+                "versions.yml:md5,fb42c6cc5c28b6f9d3384171c7b6b143"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-06-02T20:28:52.168753"
+    },
+    "with_empty_mono_versions": {
+        "content": [
+            [
+                "versions.yml:md5,05a74dacbcd6c5906faeca3206f6563f",
+                "versions.yml:md5,52a98b45c3cf73f9314dbe9b34eca9d8",
+                "versions.yml:md5,5d86cda262f5c21e43738433a2781ebc",
+                "versions.yml:md5,608d746d45680f025562c4be455db461",
+                "versions.yml:md5,8468404805dddec679524e75b4fa51e8",
+                "versions.yml:md5,9b2d5d76add82441b1e950d333e31307",
+                "versions.yml:md5,fb42c6cc5c28b6f9d3384171c7b6b143"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-06-02T20:35:11.879068"
+    },
+    "with_mono_versions": {
+        "content": [
+            [
+                "versions.yml:md5,05a74dacbcd6c5906faeca3206f6563f",
+                "versions.yml:md5,52a98b45c3cf73f9314dbe9b34eca9d8",
+                "versions.yml:md5,5d86cda262f5c21e43738433a2781ebc",
+                "versions.yml:md5,608d746d45680f025562c4be455db461",
+                "versions.yml:md5,8468404805dddec679524e75b4fa51e8",
+                "versions.yml:md5,9b2d5d76add82441b1e950d333e31307",
+                "versions.yml:md5,fb42c6cc5c28b6f9d3384171c7b6b143"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-06-02T20:30:58.393054"
+    },
+    "empty_fasta_stub": {
+        "content": [
+            {
+                "0": [
+
+                ],
+                "1": [
+
+                ],
+                "2": [
+
+                ],
+                "3": [
+
+                ],
+                "4": [
+
+                ],
+                "annotation_gff": [
+
+                ],
+                "lai_log": [
+
+                ],
+                "lai_out": [
+
+                ],
+                "ltrlib": [
+
+                ],
+                "versions": [
+
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.1"
+        },
+        "timestamp": "2024-06-02T20:35:25.114358"
+    }
+}
diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf
index 17e0c77f..1fa329ae 100644
--- a/workflows/assemblyqc.nf
+++ b/workflows/assemblyqc.nf
@@ -515,7 +515,13 @@ workflow ASSEMBLYQC {
                                                 | map { meta, mono -> [ meta.id, mono ] },
                                                 remainder: true
                                             )
-                                            | filter { id, fasta, mono -> fasta != null }
+                                            // Danger! This partial join can fail
+                                            | filter { id, fasta, mono -> fasta }
+                                            // This filter safeguards against fail on upstream
+                                            // process failure: https://github.com/nextflow-io/nextflow/issues/5043
+                                            // fasta comes from upstream processes
+                                            // mono comes from input params, it is optional
+                                            // and may not be present for some of the combinations
                                             | map { id, fasta, mono -> [ id, fasta, mono ?: [] ] }
 
     FASTA_LTRRETRIEVER_LAI(
@@ -526,6 +532,8 @@ workflow ASSEMBLYQC {
 
     ch_lai_outputs                          = FASTA_LTRRETRIEVER_LAI.out.lai_log
                                             | join(FASTA_LTRRETRIEVER_LAI.out.lai_out, remainder: true)
+                                            // This partial join can't fail because both outputs are
+                                            // from the same process
                                             | map { meta, log, out -> out ? [ log, out ] : [log] }
 
     ch_versions                             = ch_versions.mix(FASTA_LTRRETRIEVER_LAI.out.versions)

From 04fd4a48de81794b7a0de58c836403aecfae839f Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Mon, 3 Jun 2024 21:01:28 +1200
Subject: [PATCH 73/76] Now copying files in NCBI_FCS_GX_SETUP_SAMPLE rather
 than symlinking in an attempt to support NextFlow Fusion

---
 CHANGELOG.md                              | 1 +
 modules/local/ncbi_fcs_gx_setup_sample.nf | 2 +-
 2 files changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a7393fc5..67bfcd74 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -37,6 +37,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 8. Updated `FASTA_LTRRETRIEVER_LAI` to fix a pipeline crash when `ch_monoploid_seqs` was `[ meta, [] ]` [#83](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/83)
 9. Improved input assembly documentation [#86](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/86)
 10. Added assembly tag to synteny warning message regarding missing `synteny_labels` file
+11. Now copying files in `NCBI_FCS_GX_SETUP_SAMPLE` rather than symlinking in an attempt to support NextFlow Fusion
 
 ### `Dependencies`
 
diff --git a/modules/local/ncbi_fcs_gx_setup_sample.nf b/modules/local/ncbi_fcs_gx_setup_sample.nf
index 8b36ad8f..f486c010 100644
--- a/modules/local/ncbi_fcs_gx_setup_sample.nf
+++ b/modules/local/ncbi_fcs_gx_setup_sample.nf
@@ -22,7 +22,7 @@ process NCBI_FCS_GX_SETUP_SAMPLE {
         error "NCBI_FCS_GX_SETUP_SAMPLE module does not support Conda. Please use Docker / Singularity / Podman instead."
     }
     """
-    ln -s $fasta_file "fasta.file.for.${asm_tag}.fasta"
+    cp $fasta_file fasta.file.for.${asm_tag}.fasta
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From b101564db529efe24cc8ccb2d1ea61e7750ccf2d Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 4 Jun 2024 13:15:59 +1200
Subject: [PATCH 74/76] Updated FASTA_LTRRETRIEVER_LAI

---
 CHANGELOG.md                                    | 2 +-
 modules.json                                    | 2 +-
 subworkflows/pfr/fasta_ltrretriever_lai/main.nf | 5 ++---
 3 files changed, 4 insertions(+), 5 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 67bfcd74..9ae1f69e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0 - [03-June-2024]
+## v2.0.0 - [04-June-2024]
 
 ### `Added`
 
diff --git a/modules.json b/modules.json
index 51abb62d..fe8f75cc 100644
--- a/modules.json
+++ b/modules.json
@@ -121,7 +121,7 @@
                     },
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
-                        "git_sha": "d57f5fb09ab3edca3d4e16dd41beb684a7192d04",
+                        "git_sha": "734297c99ae8b13ed73e3ff9a8f1215e1ca05ecd",
                         "installed_by": ["subworkflows"]
                     },
                     "fastq_bwa_mem_samblaster": {
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
index 09a293fc..b63f8ea1 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
@@ -19,8 +19,7 @@ workflow FASTA_LTRRETRIEVER_LAI {
     ch_versions                     = Channel.empty()
 
     // Prapre input channels
-    ch_monoploid_seqs_plain         = ch_monoploid_seqs
-                                    ?: Channel.empty()
+    ch_monoploid_seqs_plain         = ( ch_monoploid_seqs ?: Channel.empty() )
                                     | filter { meta2, seqs -> seqs }
                                     // Cater to channel: [ meta2, [] ]
                                     | map { meta2, seqs -> [ meta2.id, seqs ] }
@@ -124,7 +123,7 @@ workflow FASTA_LTRRETRIEVER_LAI {
                                     // fasta may come from upstream processes
                                     // seqs also comes from upstream processes, it is optional
                                     // and may not be present for some of the combinations
-                                    | map { id, fasta, seqs -> [ id, fasta, seqs ?: [] ] }
+                                    | map { meta, fasta, seqs -> [ meta, fasta, seqs ?: [] ] }
 
     ch_lai_inputs                   = skip_lai
                                     ? Channel.empty()

From 8a6885c80f0a16a0dfdd9240da98c5f44b174b1a Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 4 Jun 2024 14:34:36 +1200
Subject: [PATCH 75/76] fasta_ltrretriever_lai now supports stub

---
 modules.json                                  |  4 +-
 modules/pfr/custom/shortenfastaids/main.nf    |  5 +-
 .../shortenfastaids/tests/main.nf.test.snap   |  8 +-
 .../pfr/fasta_ltrretriever_lai/main.nf        | 10 +--
 .../fasta_ltrretriever_lai/tests/main.nf.test |  4 +-
 .../tests/main.nf.test.snap                   | 76 +++++++++++++++----
 6 files changed, 80 insertions(+), 27 deletions(-)

diff --git a/modules.json b/modules.json
index fe8f75cc..c0c4132a 100644
--- a/modules.json
+++ b/modules.json
@@ -42,7 +42,7 @@
                     },
                     "custom/shortenfastaids": {
                         "branch": "main",
-                        "git_sha": "e226301053a2b0f9a6af514b454b3ae7c91daa80",
+                        "git_sha": "e9ec2e4c48ead3d760c8e13950c568c19ae86b8b",
                         "installed_by": ["fasta_ltrretriever_lai"]
                     },
                     "gffread": {
@@ -121,7 +121,7 @@
                     },
                     "fasta_ltrretriever_lai": {
                         "branch": "main",
-                        "git_sha": "734297c99ae8b13ed73e3ff9a8f1215e1ca05ecd",
+                        "git_sha": "e9ec2e4c48ead3d760c8e13950c568c19ae86b8b",
                         "installed_by": ["subworkflows"]
                     },
                     "fastq_bwa_mem_samblaster": {
diff --git a/modules/pfr/custom/shortenfastaids/main.nf b/modules/pfr/custom/shortenfastaids/main.nf
index cef0796c..3cdaa07c 100644
--- a/modules/pfr/custom/shortenfastaids/main.nf
+++ b/modules/pfr/custom/shortenfastaids/main.nf
@@ -25,7 +25,10 @@ process CUSTOM_SHORTENFASTAIDS {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch "${meta.id}.short.ids.tsv"
+    echo \\
+        'IDs have acceptable length and character. No change required.' \\
+        > ${meta.id}.short.ids.tsv
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         python: \$(python --version | cut -d' ' -f2)
diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
index 49fa2afb..2506ebd6 100644
--- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
+++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap
@@ -59,7 +59,7 @@
                         {
                             "id": "test"
                         },
-                        "test.short.ids.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.short.ids.tsv:md5,fcf920d9a7b57a1e3c29a9e88673330f"
                     ]
                 ],
                 "2": [
@@ -73,7 +73,7 @@
                         {
                             "id": "test"
                         },
-                        "test.short.ids.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.short.ids.tsv:md5,fcf920d9a7b57a1e3c29a9e88673330f"
                     ]
                 ],
                 "versions": [
@@ -83,9 +83,9 @@
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.04.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-06-02T20:54:36.815875"
+        "timestamp": "2024-06-04T13:58:30.161542"
     },
     "homo_sapiens-genome_fasta-no_change": {
         "content": [
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
index b63f8ea1..3820e69c 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf
@@ -174,23 +174,23 @@ workflow FASTA_LTRRETRIEVER_LAI {
 
 def map_monoploid_seqs_to_new_ids(id, short_ids_tsv, monoploid_seqs) {
 
-    def short_ids_head = short_ids_tsv.text.split('\n')[0]
+    def short_ids_head = short_ids_tsv.text.tokenize('\n')[0]
 
     if (short_ids_head == "IDs have acceptable length and character. No change required.") {
-        return [ "${id}.mapped.monoploid.seqs.txt" ] + monoploid_seqs.text.split('\n')
+        return [ "${id}.mapped.monoploid.seqs.txt" ] + monoploid_seqs.text.tokenize('\n')
     }
 
     def orig_to_new_ids = [:]
     short_ids_tsv.text.eachLine { line ->
-        def (original_id, renamed_id) = line.split('\t')
+        def (original_id, renamed_id) = line.tokenize('\t')
         orig_to_new_ids[original_id] = renamed_id
     }
 
     def mapped_ids = []
     monoploid_seqs.text.eachLine { original_id ->
         if (!orig_to_new_ids[original_id]) {
-            error "Faild to find $original_id in ${monoploid_seqs}" +
-            "The monoploid_seqs file is malformed!"
+            error "Faild to find $original_id in ${short_ids_tsv}" +
+            "\nThe short_ids_tsv file is malformed!"
         }
 
         mapped_ids.add(orig_to_new_ids[original_id])
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
index 192ccef6..a7fc65eb 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test
@@ -135,9 +135,9 @@ nextflow_workflow {
             workflow {
                 """
                 def monoploid_seqs  = new File('test.mono.seq.txt')
-                monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1")
+                monoploid_seqs.write("chr1")
 
-                input[0]            = Channel.empty()
+                input[0]            = GUNZIP.out.gunzip
                 input[1]            = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] )
                 input[2]            = false
                 """
diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap
index 4ce42ec1..e827219f 100644
--- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap
+++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test.snap
@@ -56,41 +56,91 @@
         "content": [
             {
                 "0": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.LTRlib.fa:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "1": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.out.gff3:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "2": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "3": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "4": [
-
+                    "versions.yml:md5,05a74dacbcd6c5906faeca3206f6563f",
+                    "versions.yml:md5,52a98b45c3cf73f9314dbe9b34eca9d8",
+                    "versions.yml:md5,5d86cda262f5c21e43738433a2781ebc",
+                    "versions.yml:md5,608d746d45680f025562c4be455db461",
+                    "versions.yml:md5,8468404805dddec679524e75b4fa51e8",
+                    "versions.yml:md5,fb42c6cc5c28b6f9d3384171c7b6b143"
                 ],
                 "annotation_gff": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.out.gff3:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "lai_log": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "lai_out": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "ltrlib": [
-
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.LTRlib.fa:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "versions": [
-
+                    "versions.yml:md5,05a74dacbcd6c5906faeca3206f6563f",
+                    "versions.yml:md5,52a98b45c3cf73f9314dbe9b34eca9d8",
+                    "versions.yml:md5,5d86cda262f5c21e43738433a2781ebc",
+                    "versions.yml:md5,608d746d45680f025562c4be455db461",
+                    "versions.yml:md5,8468404805dddec679524e75b4fa51e8",
+                    "versions.yml:md5,fb42c6cc5c28b6f9d3384171c7b6b143"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.04.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-06-02T20:35:25.114358"
+        "timestamp": "2024-06-04T13:50:48.75158"
     }
-}
+}
\ No newline at end of file

From 5474d3ab2009831047e538e373ab1ec8891acdee Mon Sep 17 00:00:00 2001
From: Usman Rashid <usman@smme.edu.pk>
Date: Tue, 4 Jun 2024 15:26:35 +1200
Subject: [PATCH 76/76] Updated docs

---
 docs/usage.md | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index b211bf27..8399a19c 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -72,8 +72,8 @@ This section provides additional information for parameters. It does not list al
 
 - `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom. If this parameter is `null`, the assemblies are ordered alphabetically. All assemblies from `input` and `synteny_xref_assemblies` are included by default. If an assembly is missing from this list, that assembly is excluded from the analysis.
 
-> [!NOTE]
-> PLOTSR performs a sequence-wise (preferably chromosome-wise) synteny analysis. The order of the sequences for each assembly is inferred from its `synteny_labels` file.
+> [!WARNING]
+> PLOTSR performs a sequence-wise (preferably chromosome-wise) synteny analysis. The order of the sequences for each assembly is inferred from its `synteny_labels` file and the order of sequences in the FASTA file is ignored. As all the assemblies are included in a single plot and the number of sequences from each assembly should be same, sequences after the common minimum number are excluded. Afterwards, the sequences are marked sequentially as `Chr1`, `Chr2`, `Chr3`,... If a label other than `Chr` is desirable, it can be configured with the `synteny_plotsr_seq_label` parameter.
 
 ### Merqury K-mer analysis