From fdaa2f9746f18e73ca7f02be5b4674b670072679 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 30 Sep 2024 18:33:47 +1300 Subject: [PATCH 01/62] Version bumped to 2.2.0+dev --- CHANGELOG.md | 13 +++++++++++++ CITATION.cff | 2 +- nextflow.config | 2 +- 3 files changed, 15 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index a0a472db..2bfa26e6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,19 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## v2.2.0+dev - [30-Sep-2024] + +### `Added` + +### `Fixed` + +### `Dependencies` + +1. Nextflow!>=23.04.0 +2. nf-validation@1.1.3 + +### `Deprecated` + ## v2.1.1 - [20-Sep-2024] ### `Added` diff --git a/CITATION.cff b/CITATION.cff index eff317d2..3b342d8c 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -25,7 +25,7 @@ authors: - family-names: "Deng" given-names: "Cecilia" title: "AssemblyQC: A Nextflow pipeline for reproducible reporting of assembly quality" -version: 2.1.1 +version: 2.2.0+dev date-released: 2024-07-30 url: "https://github.com/Plant-Food-Research-Open/assemblyqc" doi: 10.1093/bioinformatics/btae477 diff --git a/nextflow.config b/nextflow.config index b161bdd7..6960a069 100644 --- a/nextflow.config +++ b/nextflow.config @@ -283,7 +283,7 @@ manifest { description = """A Nextflow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.1.1' + version = '2.2.0+dev' doi = 'https://doi.org/10.1093/bioinformatics/btae477' } From bbb0177e199a0d9bb304ad526c54f26ca3b2c8ce Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 1 Oct 2024 13:39:44 +1300 Subject: [PATCH 02/62] Reduced the GenomeTools stats figures to 300 DPI and set synteny_mummer_min_bundle_size to 1000000 --- CHANGELOG.md | 5 ++++- bin/report_modules/parsers/genometools_gt_stat_parser.py | 2 +- nextflow.config | 2 +- nextflow_schema.json | 5 +++-- 4 files changed, 9 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2bfa26e6..0ef7601e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0+dev - [30-Sep-2024] +## v2.2.0+dev - [01-Oct-2024] ### `Added` @@ -16,6 +16,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Deprecated` +1. Reduced the GenomeTools stats figures to 300 DPI +2. Now `synteny_mummer_min_bundle_size` is set to `1000000` by default + ## v2.1.1 - [20-Sep-2024] ### `Added` diff --git a/bin/report_modules/parsers/genometools_gt_stat_parser.py b/bin/report_modules/parsers/genometools_gt_stat_parser.py index ddc3bb95..13f975a5 100644 --- a/bin/report_modules/parsers/genometools_gt_stat_parser.py +++ b/bin/report_modules/parsers/genometools_gt_stat_parser.py @@ -335,7 +335,7 @@ def create_dist_graph(groups_dict, x_label, title, file_name): arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), ) - plt.savefig(file_name, dpi=600) + plt.savefig(file_name, dpi=300) with open(file_name, "rb") as f: binary_fc = f.read() diff --git a/nextflow.config b/nextflow.config index 6960a069..02a7acb7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -62,7 +62,7 @@ params { synteny_mummer_plot_type = 'both' synteny_mummer_m2m_align = false synteny_mummer_max_gap = 1000000 - synteny_mummer_min_bundle_size = 1000 + synteny_mummer_min_bundle_size = 1000000 synteny_plot_1_vs_all = false synteny_color_by_contig = true synteny_plotsr_seq_label = 'Chr' diff --git a/nextflow_schema.json b/nextflow_schema.json index 7c8f50bd..fdb21ac2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -214,7 +214,8 @@ "hic": { "type": "string", "description": "HiC reads path provided as a SRA ID or as paired reads such as 'hic_reads{1,2}.fastq.gz'", - "pattern": "^SR\\w+$|^\\S+\\{1,2\\}[\\w\\.]*\\.f(ast)?q\\.gz$" + "pattern": "^SR\\w+$|^\\S+\\{1,2\\}[\\w\\.]*\\.f(ast)?q\\.gz$", + "fa_icon": "fas fa-copy" }, "hic_skip_fastp": { "type": "boolean", @@ -292,7 +293,7 @@ }, "synteny_mummer_min_bundle_size": { "type": "integer", - "default": 1000, + "default": 1000000, "description": "After bundling, any Mummer alignment bundle smaller than this size is filtered out", "fa_icon": "fas fa-ruler-horizontal" }, From bf337bacb94db6ff269123fcce77cc856cdd0b2d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 1 Oct 2024 13:45:04 +1300 Subject: [PATCH 03/62] Updated params --- docs/parameters.md | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/docs/parameters.md b/docs/parameters.md index de300d51..7f8e4ff0 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -85,12 +85,11 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | `synteny_mummer_plot_type` | Synteny plot type from Mummer alignments: 'dotplot', 'circos', or 'both' | `string` | both | | | | `synteny_mummer_m2m_align` | Include Mummer alignment blocks with many-to-many mappings | `boolean` | | | | | `synteny_mummer_max_gap` | Mummer alignments within this distance are bundled together | `integer` | 1000000 | | | -| `synteny_mummer_min_bundle_size` | After bundling, any Mummer alignment bundle smaller than this size is filtered out | `integer` | 1000 | | | +| `synteny_mummer_min_bundle_size` | After bundling, any Mummer alignment bundle smaller than this size is filtered out | `integer` | 1000000 | | | | `synteny_plot_1_vs_all` | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly. This only applies to Mummer plots | `boolean` | | | | | `synteny_color_by_contig` | Mummer synteny plots are colored by contig. Otherwise, they are colored by bundle size | `boolean` | True | | | | `synteny_plotsr_seq_label` | Sequence label prefix for plotsr synteny | `string` | Chr | | | -| `synteny_plotsr_assembly_order` | The order in which the assemblies should be compared, provided as space separated string of assembly tags. If absent, assemblies are ordered by their tags alphabetically. | `string` | -| | | | +| `synteny_plotsr_assembly_order` | The order in which the assemblies should be compared, provided as space separated string of assembly tags. If absent, assemblies are ordered by their tags alphabetically. | `string` | | | | ## Merqury options From 98ff46c2dff8394626b88e00173cfc5bfb2bba32 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 1 Oct 2024 17:36:38 +1300 Subject: [PATCH 04/62] Added gfastats --- CITATIONS.md | 4 + README.md | 4 +- bin/assemblyqc.py | 4 + bin/report_modules/parsers/gfastats_parser.py | 46 ++++++++++ bin/report_modules/templates/base.html | 8 ++ .../templates/gfastats/dropdown.html | 10 +++ .../templates/gfastats/gfastats.html | 16 ++++ .../templates/gfastats/report_contents.html | 17 ++++ conf/modules.config | 10 +++ conf/test_full.config | 2 + docs/output.md | 17 +++- docs/parameters.md | 1 + modules.json | 5 ++ modules/local/createreport.nf | 1 + modules/nf-core/gfastats/environment.yml | 5 ++ modules/nf-core/gfastats/main.nf | 66 +++++++++++++++ modules/nf-core/gfastats/meta.yml | 83 +++++++++++++++++++ nextflow.config | 3 +- nextflow_schema.json | 5 ++ pfr/params.json | 1 + tests/stub/params.json | 1 + workflows/assemblyqc.nf | 23 +++++ 22 files changed, 327 insertions(+), 5 deletions(-) create mode 100644 bin/report_modules/parsers/gfastats_parser.py create mode 100644 bin/report_modules/templates/gfastats/dropdown.html create mode 100644 bin/report_modules/templates/gfastats/gfastats.html create mode 100644 bin/report_modules/templates/gfastats/report_contents.html create mode 100644 modules/nf-core/gfastats/environment.yml create mode 100644 modules/nf-core/gfastats/main.nf create mode 100644 modules/nf-core/gfastats/meta.yml diff --git a/CITATIONS.md b/CITATIONS.md index fbd8036d..22ae2fb0 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -36,6 +36,10 @@ > > Forked from: +- GFASTATS, [MIT](https://github.com/vgl-hub/gfastats/blob/main/LICENSE) + + > Giulio Formenti, Linelle Abueg, Angelo Brajuka, Nadolina Brajuka, Cristóbal Gallardo-Alba, Alice Giani, Olivier Fedrigo, Erich D Jarvis, Gfastats: conversion, evaluation and manipulation of genome sequences using assembly graphs, Bioinformatics, Volume 38, Issue 17, September 2022, Pages 4214–4216, + - BUSCO, [MIT](https://gitlab.com/ezlab/busco/-/blob/master/LICENSE) > Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. 2021. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes, Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654, diff --git a/README.md b/README.md index de13e09d..7adcae92 100644 --- a/README.md +++ b/README.md @@ -42,7 +42,7 @@ flowchart LR VALIDATE_FORMAT ==> GFF_STATS[GENOMETOOLS GT STAT] - Run ==> ASS_STATS[ASSEMBLATHON STATS] + Run ==> ASS_STATS[STATS] Run ==> BUSCO Run ==> TIDK Run ==> LAI @@ -72,7 +72,7 @@ flowchart LR - [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator) + [SEQKIT RMDUP](https://github.com/shenwei356/seqkit): FASTA validation - [GENOMETOOLS GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation -- [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics +- [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl), [GFASTATS](https://github.com/vgl-hub/gfastats): Assembly statistics - [GENOMETOOLS GT STAT](https://genometools.org/tools/gt_stat.html): Annotation statistics - [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail - [NCBI FCS GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py index b5c73f56..c81db840 100755 --- a/bin/assemblyqc.py +++ b/bin/assemblyqc.py @@ -16,6 +16,9 @@ from report_modules.parsers.assemblathon_stats_parser import ( parse_assemblathon_stats_folder, ) +from report_modules.parsers.gfastats_parser import ( + parse_gfastats_folder, +) from report_modules.parsers.genometools_gt_stat_parser import ( parse_genometools_gt_stat_folder, ) @@ -41,6 +44,7 @@ data_from_tools = {**data_from_tools, **parse_ncbi_fcs_adaptor_folder()} data_from_tools = {**data_from_tools, **parse_ncbi_fcs_gx_folder()} data_from_tools = {**data_from_tools, **parse_assemblathon_stats_folder()} + data_from_tools = {**data_from_tools, **parse_gfastats_folder()} data_from_tools = {**data_from_tools, **parse_genometools_gt_stat_folder()} data_from_tools = {**data_from_tools, **parse_busco_folder()} data_from_tools = { diff --git a/bin/report_modules/parsers/gfastats_parser.py b/bin/report_modules/parsers/gfastats_parser.py new file mode 100644 index 00000000..6cfe2104 --- /dev/null +++ b/bin/report_modules/parsers/gfastats_parser.py @@ -0,0 +1,46 @@ +import os +from pathlib import Path +import pandas as pd +from tabulate import tabulate +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_gfastats_folder(folder_name="gfastats"): + dir = os.getcwdb().decode() + reports_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(reports_folder_path): + return {} + + list_of_report_files = reports_folder_path.glob("*.assembly_summary") + + data = {"GFASTATS": []} + + for report_path in list_of_report_files: + report_table = pd.read_csv(report_path, sep="\t") + report_table.columns = ['Stat', 'Value'] + + file_tokens = re.findall( + r"([\w]+).assembly_summary", + os.path.basename(str(report_path)), + )[0] + + data["GFASTATS"].append( + { + "hap": file_tokens, + "report_table": report_table.to_dict("records"), + "report_table_html": tabulate( + report_table, + headers=["Stat", "Value"], + tablefmt="html", + numalign="left", + showindex=False, + ), + } + ) + + return { + "GFASTATS": sort_list_of_results(data["GFASTATS"], "hap") + } diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html index 11fe7d92..c7c94f04 100644 --- a/bin/report_modules/templates/base.html +++ b/bin/report_modules/templates/base.html @@ -32,6 +32,10 @@ {% endif %} + {% if 'GFASTATS' in all_stats_dicts %} + + {% endif %} + {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %} {% endif %} @@ -100,6 +104,10 @@ {% include 'assemblathon_stats/assemblathon_stats.html' %} {% endif %} + {% if 'GFASTATS' in all_stats_dicts %} + {% include 'gfastats/gfastats.html' %} + {% endif %} + {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %} {% include 'genometools_gt_stat/genometools_gt_stat.html' %} {% endif %} diff --git a/bin/report_modules/templates/gfastats/dropdown.html b/bin/report_modules/templates/gfastats/dropdown.html new file mode 100644 index 00000000..e2e76f80 --- /dev/null +++ b/bin/report_modules/templates/gfastats/dropdown.html @@ -0,0 +1,10 @@ + diff --git a/bin/report_modules/templates/gfastats/gfastats.html b/bin/report_modules/templates/gfastats/gfastats.html new file mode 100644 index 00000000..eb94d1c4 --- /dev/null +++ b/bin/report_modules/templates/gfastats/gfastats.html @@ -0,0 +1,16 @@ + diff --git a/bin/report_modules/templates/gfastats/report_contents.html b/bin/report_modules/templates/gfastats/report_contents.html new file mode 100644 index 00000000..e1ca2e9a --- /dev/null +++ b/bin/report_modules/templates/gfastats/report_contents.html @@ -0,0 +1,17 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["GFASTATS"]|length) %} {% set +active_text = 'display: block' if vars.is_first else 'display: none' %} +
+
+
+
{{ all_stats_dicts['GFASTATS'][item]['hap'] }}
+
+
+
+
{{ all_stats_dicts['GFASTATS'][item]['report_table_html'] }}
+
+
+{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/conf/modules.config b/conf/modules.config index 5f857db6..efc06589 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -50,6 +50,16 @@ process { ] } + withName: GFASTATS { + ext.args = '--stats -t --nstar-report' + publishDir = [ + path: { "${params.outdir}/gfastats" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename }, + pattern: '*.assembly_summary' + ] + } + withName: FCS_FCSADAPTOR { ext.args = params.ncbi_fcs_adaptor_empire ? "--${params.ncbi_fcs_adaptor_empire}" : '--prok' diff --git a/conf/test_full.config b/conf/test_full.config index a8e663ab..14f95cb9 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -16,6 +16,8 @@ params { input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv' + gfastats_skip = false + ncbi_fcs_adaptor_skip = false ncbi_fcs_adaptor_empire = 'euk' diff --git a/docs/output.md b/docs/output.md index ae9e7f75..f5bfacff 100644 --- a/docs/output.md +++ b/docs/output.md @@ -14,7 +14,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [FASTA and GFF3 validation](#fasta-and-gff3-validation) - [Assemblathon stats](#assemblathon-stats) -- [Genometools gt stat](#genometools-gt-stat) +- [Gfastats](#gfastats) +- [GenomeTools gt stat](#genometools-gt-stat) - [NCBI FCS adaptor](#ncbi-fcs-adaptor) - [NCBI FCS GX](#ncbi-fcs-gx) - [BUSCO](#busco) @@ -45,7 +46,19 @@ The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fa > [!WARNING] > Contig-related stats are based on the assumption that `assemblathon_stats_n_limit` is specified correctly. If you are not certain of the value of `assemblathon_stats_n_limit`, please ignore the contig-related stats. -### Genometools gt stat +### Gfastats + +
+Output files + +- `gfastats/` + - `*.assembly_summary`: Assembly stats in TSV format. + +
+ +Gfastats is a fast and exhaustive tool for summary statistics. + +### GenomeTools gt stat
Output files diff --git a/docs/parameters.md b/docs/parameters.md index 7f8e4ff0..5a379490 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -21,6 +21,7 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | Parameter | Description | Type | Default | Required | Hidden | | ---------------------------- | ----------------------------------------------------------------------- | --------- | ------- | -------- | ------ | | `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size. NCBI recommendation is 100 | `integer` | 100 | | | +| `gfastats_skip` | | `boolean` | True | | | ## NCBI FCS options diff --git a/modules.json b/modules.json index 86cf046a..21d052a3 100644 --- a/modules.json +++ b/modules.json @@ -165,6 +165,11 @@ "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", "installed_by": ["modules"] }, + "gfastats": { + "branch": "master", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "installed_by": ["modules"] + }, "gunzip": { "branch": "master", "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf index fd53edc0..a7c05566 100644 --- a/modules/local/createreport.nf +++ b/modules/local/createreport.nf @@ -10,6 +10,7 @@ process CREATEREPORT { path ncbi_fcs_adaptor_reports , stageAs: 'ncbi_fcs_adaptor_reports/*' path fcs_gx_reports , stageAs: 'fcs_gx_reports/*' path assemblathon_stats , stageAs: 'assemblathon_stats/*' + path gfastats , stageAs: 'gfastats/*' path genometools_gt_stats , stageAs: 'genometools_gt_stat/*' path busco_outputs , stageAs: 'busco_outputs/*' path busco_gff_outputs , stageAs: 'busco_gff_outputs/*' diff --git a/modules/nf-core/gfastats/environment.yml b/modules/nf-core/gfastats/environment.yml new file mode 100644 index 00000000..b47bbdbb --- /dev/null +++ b/modules/nf-core/gfastats/environment.yml @@ -0,0 +1,5 @@ +channels: + - conda-forge + - bioconda +dependencies: + - bioconda::gfastats=1.3.6 diff --git a/modules/nf-core/gfastats/main.nf b/modules/nf-core/gfastats/main.nf new file mode 100644 index 00000000..8db239ad --- /dev/null +++ b/modules/nf-core/gfastats/main.nf @@ -0,0 +1,66 @@ +process GFASTATS { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gfastats:1.3.6--hdcf5f25_3': + 'biocontainers/gfastats:1.3.6--hdcf5f25_3' }" + + input: + tuple val(meta), path(assembly) // input.[fasta|fastq|gfa][.gz] + val out_fmt // output format (fasta/fastq/gfa) + val genome_size // estimated genome size for NG* statistics (optional). + val target // target specific sequence by header, optionally with coordinates (optional). + path agpfile // -a --agp-to-path converts input agp to path and replaces existing paths. + path include_bed // -i --include-bed generates output on a subset list of headers or coordinates in 0-based bed format. + path exclude_bed // -e --exclude-bed opposite of --include-bed. They can be combined (no coordinates). + path instructions // -k --swiss-army-knife set of instructions provided as an ordered list. + + output: + tuple val(meta), path("*.assembly_summary"), emit: assembly_summary + tuple val(meta), path("*.${out_fmt}.gz") , emit: assembly + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def agp = agpfile ? "--agp-to-path $agp" : "" + def ibed = include_bed ? "--include-bed $include_bed" : "" + def ebed = exclude_bed ? "--exclude-bed $exclude_bed" : "" + def sak = instructions ? "--swiss-army-knife $instructions" : "" + """ + gfastats \\ + $args \\ + --threads $task.cpus \\ + $agp \\ + $ibed \\ + $ebed \\ + $sak \\ + --out-format ${prefix}.${out_fmt}.gz \\ + $assembly \\ + $genome_size \\ + $target \\ + > ${prefix}.assembly_summary + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gfastats: \$( gfastats -v | sed '1!d;s/.*v//' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.${out_fmt}.gz + touch ${prefix}.assembly_summary + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gfastats: \$( gfastats -v | sed '1!d;s/.*v//' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/gfastats/meta.yml b/modules/nf-core/gfastats/meta.yml new file mode 100644 index 00000000..a6213433 --- /dev/null +++ b/modules/nf-core/gfastats/meta.yml @@ -0,0 +1,83 @@ +name: "gfastats" +description: | + A single fast and exhaustive tool for summary statistics and simultaneous *fa* + (fasta, fastq, gfa [.gz]) genome assembly file manipulation. +keywords: + - gfastats + - fasta + - genome assembly + - genome summary + - genome manipulation + - genome statistics +tools: + - "gfastats": + description: "The swiss army knife for genome assembly." + homepage: "https://github.com/vgl-hub/gfastats" + documentation: "https://github.com/vgl-hub/gfastats/tree/main/instructions" + tool_dev_url: "https://github.com/vgl-hub/gfastats" + doi: "10.1093/bioinformatics/btac460" + licence: ["MIT"] + identifier: biotools:gfastats +input: + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - assembly: + type: file + description: Draft assembly file + pattern: "*.{fasta,fastq,gfa}(.gz)?" + - - out_fmt: + type: string + description: Output format (fasta, fastq, gfa) + - - genome_size: + type: integer + description: estimated genome size (bp) for NG* statistics (optional). + - - target: + type: string + description: target specific sequence by header, optionally with coordinates + (optional). + - - agpfile: + type: file + description: converts input agp to path and replaces existing paths. + - - include_bed: + type: file + description: generates output on a subset list of headers or coordinates in + 0-based bed format. + - - exclude_bed: + type: file + description: opposite of --include-bed. They can be combined (no coordinates). + - - instructions: + type: file + description: set of instructions provided as an ordered list. +output: + - assembly_summary: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.assembly_summary": + type: file + description: Assembly summary statistics file + pattern: "*.assembly_summary" + - assembly: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.${out_fmt}.gz": + type: file + description: The assembly as modified by gfastats + pattern: "*.{fasta,fastq,gfa}.gz" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@mahesh-panchal" +maintainers: + - "@mahesh-panchal" diff --git a/nextflow.config b/nextflow.config index 02a7acb7..aafebe96 100644 --- a/nextflow.config +++ b/nextflow.config @@ -15,8 +15,9 @@ params { // Validation options check_sequence_duplicates = true - // Assemblathon stats options + // General stats options assemblathon_stats_n_limit = 100 + gfastats_skip = true // NCBI FCS options ncbi_fcs_adaptor_skip = true diff --git a/nextflow_schema.json b/nextflow_schema.json index fdb21ac2..71d01b8f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -62,6 +62,11 @@ "default": 100, "description": "The number of 'N's for the unknown gap size. NCBI recommendation is 100", "fa_icon": "fas fa-ruler-horizontal" + }, + "gfastats_skip": { + "type": "boolean", + "default": true, + "fa_icon": "fas fa-forward" } } }, diff --git a/pfr/params.json b/pfr/params.json index 8453604b..aabf5fba 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -2,6 +2,7 @@ "input": "/workspace/assemblyqc/testdata/v2/assemblysheet.csv", "check_sequence_duplicates": true, "assemblathon_stats_n_limit": 100, + "gfastats_skip": false, "ncbi_fcs_adaptor_skip": false, "ncbi_fcs_adaptor_empire": "euk", "ncbi_fcs_gx_skip": false, diff --git a/tests/stub/params.json b/tests/stub/params.json index 6e303ac4..573986a7 100644 --- a/tests/stub/params.json +++ b/tests/stub/params.json @@ -2,6 +2,7 @@ "config_profile_name": "Full stub test", "config_profile_description": "Full test of the pipeline in stub mode", "input": "assets/assemblysheetv2.csv", + "gfastats_skip": false, "ncbi_fcs_adaptor_skip": false, "ncbi_fcs_adaptor_empire": "euk", "ncbi_fcs_gx_skip": false, diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 8f615b3e..4dd9067b 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -15,6 +15,7 @@ include { GFF3_GT_GFF3_GFF3VALIDATOR_STAT } from '../subworkflows/gallvp/gff3_ include { FCS_FCSADAPTOR } from '../modules/nf-core/fcs/fcsadaptor/main' include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' +include { GFASTATS } from '../modules/nf-core/gfastats/main' include { FASTA_GXF_BUSCO_PLOT } from '../subworkflows/gallvp/fasta_gxf_busco_plot/main' include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/gallvp/fasta_ltrretriever_lai/main' include { FASTA_KRAKEN2 } from '../subworkflows/local/fasta_kraken2' @@ -433,6 +434,27 @@ workflow ASSEMBLYQC { ch_assemblathon_stats = ASSEMBLATHON_STATS.out.stats ch_versions = ch_versions.mix(ASSEMBLATHON_STATS.out.versions.first()) + // MODULE: GFASTATS + ch_gfastats_assembly = params.gfastats_skip + ? Channel.empty() + : ch_clean_assembly + | map { tag, fasta -> [ [ id: tag ], fasta ] } + + GFASTATS( + ch_gfastats_assembly, + 'gfa', // output format + '', // estimated genome size + '', // target specific sequence by header + [], // agp file + [], // include bed + [], // exclude bed + [] // instructions + ) + + ch_gfastats_stats = GFASTATS.out.assembly_summary + | map { tag, stats -> stats } + ch_versions = ch_versions.mix(GFASTATS.out.versions.first()) + // SUBWORKFLOW: FASTA_GXF_BUSCO_PLOT ch_busco_input_assembly = params.busco_skip ? Channel.empty() @@ -793,6 +815,7 @@ workflow ASSEMBLYQC { ch_fcs_adaptor_report .map { meta, file -> file }.collect().ifEmpty([]), ch_fcs_gx_report .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]), ch_assemblathon_stats .collect().ifEmpty([]), + ch_gfastats_stats .collect().ifEmpty([]), ch_gt_stats .collect().ifEmpty([]), ch_busco_outputs .collect().ifEmpty([]), ch_busco_gff_outputs .collect().ifEmpty([]), From fc0f98908cccaaa51b38c98121287f154a2255c5 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 1 Oct 2024 17:38:47 +1300 Subject: [PATCH 05/62] Updated changelog --- CHANGELOG.md | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 0ef7601e..436cacf8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` +1. Added Gfastats [#126](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/126) + ### `Fixed` ### `Dependencies` @@ -16,8 +18,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Deprecated` -1. Reduced the GenomeTools stats figures to 300 DPI -2. Now `synteny_mummer_min_bundle_size` is set to `1000000` by default +1. Reduced the GenomeTools stats figures to 300 DPI [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) +2. Now `synteny_mummer_min_bundle_size` is set to `1000000` by default [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) ## v2.1.1 - [20-Sep-2024] From fba2ea03e2490e654ceacdee17311d26e70c4262 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 1 Oct 2024 17:44:26 +1300 Subject: [PATCH 06/62] Added description for gfastats_skip --- docs/parameters.md | 2 +- nextflow_schema.json | 1 + 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/docs/parameters.md b/docs/parameters.md index 5a379490..c9d7eefb 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -21,7 +21,7 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | Parameter | Description | Type | Default | Required | Hidden | | ---------------------------- | ----------------------------------------------------------------------- | --------- | ------- | -------- | ------ | | `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size. NCBI recommendation is 100 | `integer` | 100 | | | -| `gfastats_skip` | | `boolean` | True | | | +| `gfastats_skip` | Skip Gfastats | `boolean` | True | | | ## NCBI FCS options diff --git a/nextflow_schema.json b/nextflow_schema.json index 71d01b8f..3c3fb30d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -65,6 +65,7 @@ }, "gfastats_skip": { "type": "boolean", + "description": "Skip Gfastats", "default": true, "fa_icon": "fas fa-forward" } From 87f4f4afb651b2bd407969795b65425d52b720de Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 17:28:36 +1300 Subject: [PATCH 07/62] Template update for nf-core/tools version 3.0.0 --- .editorconfig | 4 + .github/CONTRIBUTING.md | 12 +- .github/ISSUE_TEMPLATE/bug_report.yml | 32 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/ci.yml | 17 +- .github/workflows/download_pipeline.yml | 53 ++- .github/workflows/linting.yml | 23 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 75 --- .../workflows/template_version_comment.yml | 43 ++ .gitpod.yml | 7 +- .nf-core.yml | 37 +- .pre-commit-config.yaml | 2 +- .prettierignore | 1 + CHANGELOG.md | 2 +- CITATIONS.md | 8 - README.md | 10 +- assets/email_template.html | 150 ++++-- assets/methods_description_template.yml | 29 -- assets/multiqc_config.yml | 14 - assets/schema_input.json | 2 +- assets/sendmail_template.txt | 22 - conf/base.config | 34 +- conf/igenomes.config | 440 ------------------ conf/modules.config | 13 +- conf/test.config | 16 +- conf/test_full.config | 4 +- docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/output.md | 43 +- docs/usage.md | 14 +- main.nf | 27 +- modules.json | 21 +- modules/nf-core/fastqc/environment.yml | 7 - modules/nf-core/fastqc/main.nf | 61 --- modules/nf-core/fastqc/meta.yml | 57 --- modules/nf-core/fastqc/tests/main.nf.test | 212 --------- .../nf-core/fastqc/tests/main.nf.test.snap | 88 ---- modules/nf-core/fastqc/tests/tags.yml | 2 - modules/nf-core/multiqc/environment.yml | 7 - modules/nf-core/multiqc/main.nf | 55 --- modules/nf-core/multiqc/meta.yml | 58 --- modules/nf-core/multiqc/tests/main.nf.test | 84 ---- .../nf-core/multiqc/tests/main.nf.test.snap | 41 -- modules/nf-core/multiqc/tests/tags.yml | 2 - nextflow.config | 137 ++---- nextflow_schema.json | 141 +----- .../utils_nfcore_assemblyqc_pipeline/main.nf | 101 ++-- .../nf-core/utils_nextflow_pipeline/main.nf | 24 +- .../tests/nextflow.config | 2 +- .../nf-core/utils_nfcore_pipeline/main.nf | 45 +- .../nf-core/utils_nfschema_plugin/main.nf | 46 ++ .../nf-core/utils_nfschema_plugin/meta.yml | 35 ++ .../utils_nfschema_plugin/tests/main.nf.test | 117 +++++ .../tests/nextflow.config | 8 + .../tests/nextflow_schema.json | 8 +- .../nf-core/utils_nfvalidation_plugin/main.nf | 62 --- .../utils_nfvalidation_plugin/meta.yml | 44 -- .../tests/main.nf.test | 200 -------- .../utils_nfvalidation_plugin/tests/tags.yml | 2 - tower.yml | 2 - workflows/assemblyqc.nf | 61 +-- 63 files changed, 666 insertions(+), 2202 deletions(-) delete mode 100644 .github/workflows/release-announcements.yml create mode 100644 .github/workflows/template_version_comment.yml delete mode 100644 assets/methods_description_template.yml delete mode 100644 assets/multiqc_config.yml delete mode 100644 conf/igenomes.config delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png delete mode 100644 modules/nf-core/fastqc/environment.yml delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml delete mode 100644 modules/nf-core/multiqc/environment.yml delete mode 100644 modules/nf-core/multiqc/main.nf delete mode 100644 modules/nf-core/multiqc/meta.yml delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/multiqc/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config rename subworkflows/nf-core/{utils_nfvalidation_plugin => utils_nfschema_plugin}/tests/nextflow_schema.json (95%) delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 72dda289..e1058815 100644 --- a/.editorconfig +++ b/.editorconfig @@ -11,6 +11,7 @@ indent_style = space [*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 + # These files are edited and tested upstream in nf-core/modules [/modules/nf-core/**] charset = unset @@ -25,9 +26,12 @@ insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset + + [/assets/email*] indent_size = unset + # ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 0144e255..1391e6f0 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -16,7 +16,7 @@ If you'd like to write some code for plant-food-research-open/assemblyqc, the st 1. Check that there isn't already an issue about your idea in the [plant-food-research-open/assemblyqc issues](https://github.com/plant-food-research-open/assemblyqc/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [plant-food-research-open/assemblyqc repository](https://github.com/plant-food-research-open/assemblyqc) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) -4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +4. Use `nf-core pipelines schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). @@ -37,7 +37,7 @@ There are typically two types of tests that run: ### Lint tests `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to. -To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint ` command. +To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core pipelines lint ` command. If any failures or warnings are encountered, please follow the listed URL for more documentation. @@ -68,18 +68,16 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new parameters to `nextflow.config` with a default (see below). -5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool). +5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool). 6. Add sanity checks and validation for all relevant parameters. 7. Perform local tests to validate that the new code works as expected. 8. If applicable, add a new test command in `.github/workflow/ci.yml`. -9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module. -10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. ### Default values Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. -Once there, use `nf-core schema build` to add to `nextflow_schema.json`. +Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`. ### Default processes resource requirements @@ -96,7 +94,7 @@ Please use the following naming schemes, to make it easy to understand what is g ### Nextflow version bumping -If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]` +If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core pipelines bump-version --nextflow . [min-nf-version]` ### Images and figures diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index af436aa6..e3135746 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -9,46 +9,34 @@ body: description: A clear and concise description of what the bug is. validations: required: true + - type: textarea id: command_used attributes: label: Command used and terminal output - description: Steps to reproduce the behaviour. Please paste the command you used - to launch the pipeline and the output from your terminal. + description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal. render: console - placeholder: "$ nextflow run ... - + placeholder: | + $ nextflow run ... Some output where something broke - " - type: textarea id: files attributes: label: Relevant files - description: "Please drag and drop the relevant files here. Create a `.zip` archive - if the extension is not allowed. - - Your verbose log file `.nextflow.log` is often useful _(this is a hidden file - in the directory where you launched the pipeline)_ as well as custom Nextflow - configuration files. + description: | + Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed. + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files. - " - type: textarea id: system attributes: label: System information - description: "* Nextflow version _(eg. 23.04.0)_ - + description: | + * Nextflow version _(eg. 23.04.0)_ * Hardware _(eg. HPC, Desktop, Cloud)_ - * Executor _(eg. slurm, local, awsbatch)_ - - * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, - or Apptainer)_ - + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - * Version of plant-food-research-open/assemblyqc _(eg. 1.1, 1.5, 1.8.2)_ - - " diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 3da295ca..a70d5a05 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -16,7 +16,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/plant-food-r - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md) -- [ ] Make sure your code lints (`nf-core lint`). +- [ ] Make sure your code lints (`nf-core pipelines lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 81e48363..e020ae89 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -7,6 +7,7 @@ on: pull_request: release: types: [published] + workflow_dispatch: env: NXF_ANSI_LOG: false @@ -24,7 +25,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.04.0" + - "24.04.2" - "latest-everything" steps: - name: Check out pipeline code @@ -38,9 +39,21 @@ jobs: - name: Disk space cleanup uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - name: Run pipeline with test data + - name: Run pipeline with test data (docker) # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix run: | nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + + - name: Run pipeline with test data (singularity) + # TODO nf-core: You can customise CI pipeline run tests as required + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results + if: "${{ github.base_ref == 'master' }}" + + - name: Run pipeline with test data (conda) + # TODO nf-core: You can customise CI pipeline run tests as required + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,conda --outdir ./results + if: "${{ github.base_ref == 'master' }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 2d20d644..713dc3e7 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -1,4 +1,4 @@ -name: Test successful pipeline download with 'nf-core download' +name: Test successful pipeline download with 'nf-core pipelines download' # Run the workflow when: # - dispatched manually @@ -8,7 +8,7 @@ on: workflow_dispatch: inputs: testbranch: - description: "The specific branch you wish to utilize for the test execution of nf-core download." + description: "The specific branch you wish to utilize for the test execution of nf-core pipelines download." required: true default: "dev" pull_request: @@ -39,9 +39,11 @@ jobs: with: python-version: "3.12" architecture: "x64" - - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 + + - name: Setup Apptainer + uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0 with: - singularity-version: 3.8.3 + apptainer-version: 1.3.4 - name: Install dependencies run: | @@ -54,33 +56,64 @@ jobs: echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + - name: Make a cache directory for the container images + run: | + mkdir -p ./singularity_container_images + - name: Download the pipeline env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core pipelines download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ --container-system 'singularity' \ - --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io" \ --container-cache-utilisation 'amend' \ - --download-configuration + --download-configuration 'yes' - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} + - name: Count the downloaded number of container images + id: count_initial + run: | + image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) + echo "Initial container image count: $image_count" + echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV} + - name: Run the downloaded pipeline (stub) id: stub_run_pipeline continue-on-error: true env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - name: Run the downloaded pipeline (stub run not supported) id: run_pipeline if: ${{ job.steps.stub_run_pipeline.status == failure() }} env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results + + - name: Count the downloaded number of container images + id: count_afterwards + run: | + image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) + echo "Post-pipeline run container image count: $image_count" + echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV} + + - name: Compare container image counts + run: | + if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then + initial_count=${{ env.IMAGE_COUNT_INITIAL }} + final_count=${{ env.IMAGE_COUNT_AFTER }} + difference=$((final_count - initial_count)) + echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!" + tree ./singularity_container_images + exit 1 + else + echo "The pipeline can be downloaded successfully!" + fi diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 1fcafe88..b882838a 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -1,6 +1,6 @@ name: nf-core linting # This workflow is triggered on pushes and PRs to the repository. -# It runs the `nf-core lint` and markdown lint tests to ensure +# It runs the `nf-core pipelines lint` and markdown lint tests to ensure # that the code meets the nf-core guidelines. on: push: @@ -41,17 +41,32 @@ jobs: python-version: "3.12" architecture: "x64" + - name: read .nf-core.yml + uses: pietrobolcato/action-read-yaml@1.0.0 + id: read_yml + with: + config: ${{ github.workspace }}/.nf-core.yaml + - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core + pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} + + - name: Run nf-core pipelines lint + if: ${{ github.base_ref != 'master' }} + env: + GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} + run: nf-core -l lint_log.txt pipelines lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - - name: Run nf-core lint + - name: Run nf-core pipelines lint --release + if: ${{ github.base_ref == 'master' }} env: GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} - run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md + run: nf-core -l lint_log.txt pipelines lint --release --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - name: Save PR number if: ${{ always() }} diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 40acc23f..42e519bf 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 + uses: dawidd6/action-download-artifact@bf251b5aa9c2f7eeb574a96ee720e24f801b7c11 # v6 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml deleted file mode 100644 index 03ecfcf7..00000000 --- a/.github/workflows/release-announcements.yml +++ /dev/null @@ -1,75 +0,0 @@ -name: release-announcements -# Automatic release toot and tweet anouncements -on: - release: - types: [published] - workflow_dispatch: - -jobs: - toot: - runs-on: ubuntu-latest - steps: - - name: get topics and convert to hashtags - id: get_topics - run: | - echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT - - - uses: rzr/fediverse-action@master - with: - access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} - host: "mstdn.science" # custom host if not "mastodon.social" (default) - # GitHub event payload - # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release - message: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - - ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics - - send-tweet: - runs-on: ubuntu-latest - - steps: - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 - with: - python-version: "3.10" - - name: Install dependencies - run: pip install tweepy==4.14.0 - - name: Send tweet - shell: python - run: | - import os - import tweepy - - client = tweepy.Client( - access_token=os.getenv("TWITTER_ACCESS_TOKEN"), - access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), - consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), - consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), - ) - tweet = os.getenv("TWEET") - client.create_tweet(text=tweet) - env: - TWEET: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} - TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} - TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} - TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} - - bsky-post: - runs-on: ubuntu-latest - steps: - - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 - with: - post: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - env: - BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} - BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} - # diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml new file mode 100644 index 00000000..9dea41f0 --- /dev/null +++ b/.github/workflows/template_version_comment.yml @@ -0,0 +1,43 @@ +name: nf-core template version comment +# This workflow is triggered on PRs to check if the pipeline template version matches the latest nf-core version. +# It posts a comment to the PR, even if it comes from a fork. + +on: pull_request_target + +jobs: + template_version: + runs-on: ubuntu-latest + steps: + - name: Check out pipeline code + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + + - name: Read template version from .nf-core.yml + uses: pietrobolcato/action-read-yaml@1.0.0 + id: read_yml + with: + config: ${{ github.workspace }}/.nf-core.yml + + - name: Install nf-core + run: | + python -m pip install --upgrade pip + pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} + + - name: Check nf-core outdated + id: nf_core_outdated + run: pip list --outdated | grep nf-core + + - name: Post nf-core template version comment + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 + if: | + ${{ steps.nf_core_outdated.outputs.stdout }} =~ 'nf-core' + with: + repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }} + allow-repeats: false + message: | + ## :warning: Newer version of the nf-core template is available. + + Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}. + Please update your pipeline to the latest version. + + For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync). + # diff --git a/.gitpod.yml b/.gitpod.yml index 105a1821..46118637 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,17 +4,14 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - - name: unset JAVA_TOOL_OPTIONS - command: | - unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code + #- esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code - charliermarsh.ruff # Code linter Ruff diff --git a/.nf-core.yml b/.nf-core.yml index 12896981..56ab1ec3 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,5 +1,12 @@ +bump_version: null lint: files_exist: + - conf/igenomes.config + - conf/igenomes_ignored.config + - assets/multiqc_config.yml + - conf/igenomes.config + - conf/igenomes_ignored.config + - assets/multiqc_config.yml - CODE_OF_CONDUCT.md - assets/nf-core-assemblyqc_logo_light.png - docs/images/nf-core-assemblyqc_logo_light.png @@ -8,18 +15,38 @@ lint: - .github/workflows/awstest.yml - .github/workflows/awsfulltest.yml files_unchanged: + - .github/CONTRIBUTING.md + - assets/sendmail_template.txt + - .github/CONTRIBUTING.md + - assets/sendmail_template.txt - CODE_OF_CONDUCT.md - assets/nf-core-assemblyqc_logo_light.png - docs/images/nf-core-assemblyqc_logo_light.png - docs/images/nf-core-assemblyqc_logo_dark.png - .github/ISSUE_TEMPLATE/bug_report.yml - multiqc_config: - - report_comment + multiqc_config: false nextflow_config: - manifest.name - manifest.homePage -nf_core_version: 2.14.1 + - validation.help.beforeText + - validation.help.afterText + - validation.summary.beforeText + - validation.summary.afterText +nf_core_version: 3.0.0 +org_path: null repository_type: pipeline template: - prefix: plant-food-research-open - skip: [] + author: Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy + description: A NextFlow pipeline which evaluates assembly quality with multiple + QC tools and presents the results in a unified html report. + force: true + is_nfcore: false + name: assemblyqc + org: plant-food-research-open + outdir: . + skip_features: + - igenomes + - multiqc + - fastqc + version: 1.0.0dev +update: null diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 4dc0f1dc..9e9f0e1c 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -7,7 +7,7 @@ repos: - prettier@3.2.5 - repo: https://github.com/editorconfig-checker/editorconfig-checker.python - rev: "2.7.3" + rev: "3.0.3" hooks: - id: editorconfig-checker alias: ec diff --git a/.prettierignore b/.prettierignore index 437d763d..610e5069 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,3 +1,4 @@ + email_template.html adaptivecard.json slackreport.json diff --git a/CHANGELOG.md b/CHANGELOG.md index c0ebecc9..7f0eabfe 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.0.0+dev - [date] +## v1.0.0+dev - [date] Initial release of plant-food-research-open/assemblyqc, created with the [nf-core](https://nf-co.re/) template. diff --git a/CITATIONS.md b/CITATIONS.md index 2b468811..06e37ece 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -10,14 +10,6 @@ ## Pipeline tools -- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - -- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) - - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. - ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/README.md b/README.md index 5ea7ea72..73512f1d 100644 --- a/README.md +++ b/README.md @@ -1,8 +1,10 @@ +# plant-food-research-open/assemblyqc + [![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/ci.yml/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/linting.yml/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions/workflows/linting.yml)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -22,9 +24,6 @@ workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. --> -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) - ## Usage > [!NOTE] @@ -58,8 +57,7 @@ nextflow run plant-food-research-open/assemblyqc \ ``` > [!WARNING] -> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; -> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files). ## Credits diff --git a/assets/email_template.html b/assets/email_template.html index 5a56215f..49545d50 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,53 +1,119 @@ - - - - + + + + - - plant-food-research-open/assemblyqc Pipeline Report - - -
+ + plant-food-research-open/assemblyqc Pipeline Report + + +
+ - +

plant-food-research-open/assemblyqc ${version}

+

Run Name: $runName

-

plant-food-research-open/assemblyqc ${version}

-

Run Name: $runName

- -<% if (!success){ - out << """ -
-

plant-food-research-open/assemblyqc execution completed unsuccessfully!

+ <% if (!success){ out << """ +
+

+ plant-food-research-open/assemblyqc execution completed unsuccessfully! +

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

The full error message was:

-
${errorReport}
-
- """ -} else { - out << """ -
+
${errorReport}
+
+ """ } else { out << """ +
plant-food-research-open/assemblyqc execution completed successfully! -
- """ -} -%> +
+ """ } %> -

The workflow was completed at $dateComplete (duration: $duration)

-

The command used to launch the workflow was as follows:

-
$commandLine
+

The workflow was completed at $dateComplete (duration: $duration)

+

The command used to launch the workflow was as follows:

+
+$commandLine
-

Pipeline Configuration:

- - - <% out << summary.collect{ k,v -> "" }.join("\n") %> - -
$k
$v
+

Pipeline Configuration:

+ + + <% out << summary.collect{ k,v -> " + + + + + " }.join("\n") %> + +
+ $k + +
$v
+
-

plant-food-research-open/assemblyqc

-

https://github.com/plant-food-research-open/assemblyqc

- -
- - +

plant-food-research-open/assemblyqc

+

+ https://github.com/plant-food-research-open/assemblyqc +

+
+ diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml deleted file mode 100644 index e2a5645c..00000000 --- a/assets/methods_description_template.yml +++ /dev/null @@ -1,29 +0,0 @@ -id: "plant-food-research-open-assemblyqc-methods-description" -description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "plant-food-research-open/assemblyqc Methods Description" -section_href: "https://github.com/plant-food-research-open/assemblyqc" -plot_type: "html" -## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline -## You inject any metadata in the Nextflow '${workflow}' object -data: | -

Methods

-

Data was processed using plant-food-research-open/assemblyqc v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

-

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

-
${workflow.commandLine}
-

${tool_citations}

-

References

-
    -
  • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
  • -
  • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
  • -
  • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
  • -
  • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
  • - ${tool_bibliography} -
-
-
Notes:
-
    - ${nodoi_text} -
  • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
  • -
  • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
  • -
-
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml deleted file mode 100644 index 0015bfa8..00000000 --- a/assets/multiqc_config.yml +++ /dev/null @@ -1,14 +0,0 @@ -report_comment: > - This report has been generated by the plant-food-research-open/assemblyqc - analysis pipeline. -report_section_order: - "plant-food-research-open-assemblyqc-methods-description": - order: -1000 - software_versions: - order: -1001 - "plant-food-research-open-assemblyqc-summary": - order: -1002 - -export_plots: true - -disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 2a9a79ca..82c757f3 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,5 +1,5 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/assets/schema_input.json", "title": "plant-food-research-open/assemblyqc pipeline - params.input schema", "description": "Schema for the file provided with params.input", diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 0ba5f393..20644943 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -26,28 +26,6 @@ Content-Disposition: inline; filename="plant-food-research-open-assemblyqc_logo_ join( '\n' ) %> <% -if (mqcFile){ -def mqcFileObj = new File("$mqcFile") -if (mqcFileObj.length() < mqcMaxSize){ -out << """ ---nfcoremimeboundary -Content-Type: text/html; name=\"multiqc_report\" -Content-Transfer-Encoding: base64 -Content-ID: -Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" - -${mqcFileObj. - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' )} -""" -}} %> --nfcoremimeboundary-- diff --git a/conf/base.config b/conf/base.config index faaec489..674a6905 100644 --- a/conf/base.config +++ b/conf/base.config @@ -11,9 +11,9 @@ process { // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 1 * task.attempt } + memory = { 6.GB * task.attempt } + time = { 4.h * task.attempt } errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' } maxRetries = 1 @@ -27,30 +27,30 @@ process { // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { - cpus = { check_max( 1 , 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 1 } + memory = { 6.GB * task.attempt } + time = { 4.h * task.attempt } } withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 2 * task.attempt } + memory = { 12.GB * task.attempt } + time = { 4.h * task.attempt } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } + cpus = { 6 * task.attempt } + memory = { 36.GB * task.attempt } + time = { 8.h * task.attempt } } withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72.GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } + cpus = { 12 * task.attempt } + memory = { 72.GB * task.attempt } + time = { 16.h * task.attempt } } withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { 20.h * task.attempt } } withLabel:process_high_memory { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } + memory = { 200.GB * task.attempt } } withLabel:error_ignore { errorStrategy = 'ignore' diff --git a/conf/igenomes.config b/conf/igenomes.config deleted file mode 100644 index 3f114377..00000000 --- a/conf/igenomes.config +++ /dev/null @@ -1,440 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for iGenomes paths -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines reference genomes using iGenome paths. - Can be used by any config that customises the base path using: - $params.igenomes_base / --igenomes_base ----------------------------------------------------------------------------------------- -*/ - -params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" - } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'CHM13' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" - bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" - gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" - mito_name = "chrM" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_5.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } -} diff --git a/conf/modules.config b/conf/modules.config index d203d2b6..3ca9a37a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,17 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: FASTQC { - ext.args = '--quiet' - } - - withName: 'MULTIQC' { - ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } - publishDir = [ - path: { "${params.outdir}/multiqc" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + } diff --git a/conf/test.config b/conf/test.config index 6bfdc87a..8b08d5cf 100644 --- a/conf/test.config +++ b/conf/test.config @@ -10,20 +10,22 @@ ---------------------------------------------------------------------------------------- */ +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + params { config_profile_name = 'Test profile' config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.GB' - max_time = '6.h' - // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - // Genome references - genome = 'R64-1-1' + } diff --git a/conf/test_full.config b/conf/test_full.config index 931bacf5..848e90b7 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -19,6 +19,6 @@ params { // TODO nf-core: Give any required params for the test so that command line flags are not needed input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - // Genome references - genome = 'R64-1-1' + // Fasta references + fasta = params.pipelines_testdata_base_path + 'viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' } diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png deleted file mode 100755 index 361d0e47acfb424dea1f326590d1eb2f6dfa26b5..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 23458 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zXwhtT%ei{n#FzEH|C;yZ>+$!u_x#*+`=L8{b9SH^9&27u3G_Gxqxe`L2UJtdxghk z&-wzDFvLvW{chK5u3{n6GSKKy!P&C6w^IFpbD0bcp^A{{2lcLh_DXj@ybtYvc^;(2 M)78&qol`;+0Fu7JivR!s diff --git a/docs/output.md b/docs/output.md index dd6b3c21..d5e6237d 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,7 +2,7 @@ ## Introduction -This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +This document describes the output produced by the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -12,49 +12,8 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC -- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -### FastQC - -
-Output files - -- `fastqc/` - - `*_fastqc.html`: FastQC report containing quality metrics. - - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. - -
- -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) - -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) - -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) - -:::note -The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -::: - -### MultiQC - -
-Output files - -- `multiqc/` - - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - - `multiqc_plots/`: directory containing static images from the report in various formats. - -
- -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. - -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . - ### Pipeline information
-
-AssemblyQC - Spectra-cn plot -AssemblyQC - Plotsr synteny plot -
-AssemblyQC - Merqury plots -
+GenomeTools `gt stat` tool calculates a basic set of statistics about features contained in GFF3 files. + +
AssemblyQC - GenomeTools gt stat gene length distribution
AssemblyQC - GenomeTools gt stat gene length distribution
+ +### OrthoFinder + +
+Output files + +- `orthofinder/assemblyqc`: OrthoFinder output folder. + +
+ +If more than one assemblies are included along with their annotations, OrthoFinder is executed on the annotation proteins to perform a phylogenetic orthology inference for comparative genomics. + +
AssemblyQC - OrthoFinder species tree
AssemblyQC - OrthoFinder species tree
### Pipeline information diff --git a/docs/parameters.md b/docs/parameters.md index 7ef7843a..1c8fd3f6 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -34,6 +34,15 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | `ncbi_fcs_gx_db_path` | Path to NCBI FCS GX database. See: https://github.com/ncbi/fcs/wiki/FCS-GX | `string` | | | | | `contamination_stops_pipeline` | Skip remaining QC steps for an assembly which has adaptor or GX contamination | `boolean` | True | | | +## tidk options + +| Parameter | Description | Type | Default | Required | Hidden | +| --------------------- | ---------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `tidk_skip` | Skip telomere identification | `boolean` | True | | | +| `tidk_repeat_seq` | Telomere repeat sequence. Typical values for plant: TTTAGGG, fungus, vertebrates: TTAGGG and Insect: TTAGG | `string` | | | | +| `tidk_filter_by_size` | Filter assembly sequences smaller than the specified length | `boolean` | | | | +| `tidk_filter_size_bp` | Filter size in base-pairs | `integer` | 1000000 | | | + ## BUSCO options | Parameter | Description | Type | Default | Required | Hidden | @@ -43,22 +52,13 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | `busco_lineage_datasets` | BUSCO lineages. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' | `string` | | | | | `busco_download_path` | Download path for BUSCO | `string` | | | | -## TIDK options - -| Parameter | Description | Type | Default | Required | Hidden | -| --------------------- | ---------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | -| `tidk_skip` | Skip telomere identification | `boolean` | True | | | -| `tidk_repeat_seq` | Telomere repeat sequence. Typical values for plant: TTTAGGG, fungus, vertebrates: TTAGGG and Insect: TTAGG | `string` | | | | -| `tidk_filter_by_size` | Filter assembly sequences smaller than the specified length | `boolean` | | | | -| `tidk_filter_size_bp` | Filter size in base-pairs | `integer` | 1000000 | | | - ## LAI options | Parameter | Description | Type | Default | Required | Hidden | | ---------- | ------------------- | --------- | ------- | -------- | ------ | | `lai_skip` | Skip LAI estimation | `boolean` | True | | | -## Kraken2 options +## Kraken 2 options | Parameter | Description | Type | Default | Required | Hidden | | ----------------- | --------------------- | --------- | ------- | -------- | ------ | @@ -75,6 +75,13 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | `hic_fastp_ext_args` | Additional parameters for fastp trimming | `string` | --qualified_quality_phred 20 --length_required 50 | | | | `hic_samtools_ext_args` | Additional parameters for samtools view command run after samblaster | `string` | -F 3852 | | | +## Merqury options + +| Parameter | Description | Type | Default | Required | Hidden | +| --------------------- | -------------------------------- | --------- | ------- | -------- | ------ | +| `merqury_skip` | Skip merqury analysis | `boolean` | True | | | +| `merqury_kmer_length` | kmer length for merqury analysis | `integer` | 21 | | | + ## Synteny options | Parameter | Description | Type | Default | Required | Hidden | @@ -93,14 +100,7 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | `synteny_plotsr_seq_label` | Sequence label prefix for plotsr synteny | `string` | Chr | | | | `synteny_plotsr_assembly_order` | The order in which the assemblies should be compared, provided as space separated string of assembly tags. If absent, assemblies are ordered by their tags alphabetically. | `string` | | | | -## Merqury options - -| Parameter | Description | Type | Default | Required | Hidden | -| --------------------- | -------------------------------- | --------- | ------- | -------- | ------ | -| `merqury_skip` | Skip merqury analysis | `boolean` | True | | | -| `merqury_kmer_length` | kmer length for merqury analysis | `integer` | 21 | | | - -## Orthofinder options +## OrthoFinder options | Parameter | Description | Type | Default | Required | Hidden | | ------------------ | ---------------- | --------- | ------- | -------- | ------ | diff --git a/docs/usage.md b/docs/usage.md index 3e8b314b..02b198b3 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -2,17 +2,17 @@ - [Assemblysheet input](#assemblysheet-input) - [External databases](#external-databases) - - [NCBI FCS GX database](#ncbi-fcs-gx-database) - - [Kraken2](#kraken2) + - [NCBI FCS-GX database](#ncbi-fcs-gx-database) - [BUSCO](#busco) + - [Kraken 2](#kraken-2) - [Other parameters](#other-parameters) - [Assemblathon stats](#assemblathon-stats) - - [NCBI FCS GX](#ncbi-fcs-gx) + - [NCBI FCS-GX](#ncbi-fcs-gx) + - [tidk](#tidk) - [BUSCO](#busco-1) - - [TIDK](#tidk) - [HiC](#hic) - - [Synteny analysis](#synteny-analysis) - [Merqury K-mer analysis](#merqury-k-mer-analysis) + - [Synteny analysis](#synteny-analysis) - [Minimum System Requirements](#minimum-system-requirements) - [Running the pipeline](#running-the-pipeline) - [Updating the pipeline](#updating-the-pipeline) @@ -43,9 +43,9 @@ See the [Merqury](#merqury-k-mer-analysis) section For description of assemblysh ## External databases -### NCBI FCS GX database +### NCBI FCS-GX database -If NCBI FCS GX foreign organism contamination check is executed by setting `ncbi_fcs_gx_skip` to `false`, the path to the GX database must be provided with option `ncbi_fcs_gx_db_path`. The user must ensure that the database is correctly downloaded and placed in a location accessible to the pipeline. Setup instructions are available at . The database path must contain following files: +If NCBI FCS-GX foreign organism contamination check is executed by setting `ncbi_fcs_gx_skip` to `false`, the path to the GX database must be provided with option `ncbi_fcs_gx_db_path`. The user must ensure that the database is correctly downloaded and placed in a location accessible to the pipeline. Setup instructions are available at . The database path must contain following files: ```bash all.assemblies.tsv @@ -59,14 +59,14 @@ all.seq_info.tsv.gz all.taxa.tsv ``` -### Kraken2 - -Path to Kraken2 database is provided by the `kraken2_db_path` parameter. This can be a URL to a public `.tar.gz` file such as `https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz`. The pipeline can download and extract the database. This is not the recommended practice owing to the size of the database. Rather, the database should be downloaded, extracted and stored in a read-only location. The path to that location can be provided by the `kraken2_db_path` parameter such as `/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314`. - ### BUSCO BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A persistent location for the database can be provided by specifying `busco_download_path` parameter. +### Kraken 2 + +Path to Kraken 2 database is provided by the `kraken2_db_path` parameter. This can be a URL to a public `.tar.gz` file such as `https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz`. The pipeline can download and extract the database. This is not the recommended practice owing to the size of the database. Rather, the database should be downloaded, extracted and stored in a read-only location. The path to that location can be provided by the `kraken2_db_path` parameter such as `/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314`. + ## Other parameters This section provides additional information for parameters. It does not list all the pipeline parameters. For an exhaustive list, see [parameters.md](./parameters.md). @@ -75,35 +75,22 @@ This section provides additional information for parameters. It does not list al `assemblathon_stats_n_limit` is the number of 'N's for the unknown gap size. This number is used to split the scaffolds into contigs to compute contig-related stats. NCBI's recommendation for unknown gap size is 100 . -### NCBI FCS GX +### NCBI FCS-GX - `ncbi_fcs_gx_tax_id` is the taxonomy ID for all the assemblies listed in the assemblysheet. A taxonomy ID can be obtained by searching a _Genus species_ at . -### BUSCO +### tidk -- `busco_lineage_datasets`: A space-separated list of BUSCO lineages. Any number of lineages can be specified such as "fungi_odb10 hypocreales_odb10". Each assembly is assessed against each of the listed lineage. To select a lineage, refer to . +- `tidk_repeat_seq`: The telomere search sequence. To select an appropriate sequence, see . Commonly used sequences are TTTAGGG (Plant), TTAGGG (Fungus, Vertebrates) and TTAGG (Insect). Further reading: -### TIDK +### BUSCO -- `tidk_repeat_seq`: The telomere search sequence. To select an appropriate sequence, see . Commonly used sequences are TTTAGGG (Plant), TTAGGG (Fungus, Vertebrates) and TTAGG (Insect). Further reading: +- `busco_lineage_datasets`: A space-separated list of BUSCO lineages. Any number of lineages can be specified such as "fungi_odb10 hypocreales_odb10". Each assembly is assessed against each of the listed lineage. To select a lineage, refer to . ### HiC - `hic`: Path to reads provided as a SRA ID or as a path to paired reads such as 'hic_reads{1,2}.fastq.gz'. These reads are applied to each assembly listed by `input`. -### Synteny analysis - -- `synteny_xref_assemblies`: Similar to `--input`, this parameter also provides a CSV sheet listing external reference assemblies which are included in the synteny analysis but are not analysed by other QC tools. See the [example xrefsheet](../assets/xrefsheet.csv) included with the pipeline. Its fields are: - - - `tag:` A unique tag which represents the reference assembly in the final report - - `fasta:` FASTA file - - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) - -- `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom. If this parameter is `null`, the assemblies are ordered alphabetically. All assemblies from `input` and `synteny_xref_assemblies` are included by default. If an assembly is missing from this list, that assembly is excluded from the analysis. - -> [!WARNING] -> PLOTSR performs a sequence-wise (preferably chromosome-wise) synteny analysis. The order of the sequences for each assembly is inferred from its `synteny_labels` file and the order of sequences in the FASTA file is ignored. As all the assemblies are included in a single plot and the number of sequences from each assembly should be same, sequences after the common minimum number are excluded. Afterwards, the sequences are marked sequentially as `Chr1`, `Chr2`, `Chr3`,... If a label other than `Chr` is desirable, it can be configured with the `synteny_plotsr_seq_label` parameter. - ### Merqury K-mer analysis Additional assemblysheet columns: @@ -124,6 +111,19 @@ See following assemblysheet examples for MERQURY analysis. The data for these examples comes from: [umd.edu](https://obj.umiacs.umd.edu/marbl_publications/triobinning/index.html) +### Synteny analysis + +- `synteny_xref_assemblies`: Similar to `--input`, this parameter also provides a CSV sheet listing external reference assemblies which are included in the synteny analysis but are not analysed by other QC tools. See the [example xrefsheet](../assets/xrefsheet.csv) included with the pipeline. Its fields are: + + - `tag:` A unique tag which represents the reference assembly in the final report + - `fasta:` FASTA file + - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and their labels for the synteny plots (second column) + +- `synteny_plotsr_assembly_order`: The order in which Minimap2 alignments are performed and, then, plotted by Plotsr. For assembly A, B and C; if the order is specified as 'B C A', then, two alignments are performed. First, C is aligned against B as reference. Second, A is aligned against C as reference. The order of these assemblies on the Plotsr figure is also 'B C A' so that B appears on top, C in the middle and A at the bottom. If this parameter is `null`, the assemblies are ordered alphabetically. All assemblies from `input` and `synteny_xref_assemblies` are included by default. If an assembly is missing from this list, that assembly is excluded from the analysis. + +> [!WARNING] +> PLOTSR performs a sequence-wise (preferably chromosome-wise) synteny analysis. The order of the sequences for each assembly is inferred from its `synteny_labels` file and the order of sequences in the FASTA file is ignored. As all the assemblies are included in a single plot and the number of sequences from each assembly should be same, sequences after the common minimum number are excluded. Afterwards, the sequences are marked sequentially as `Chr1`, `Chr2`, `Chr3`,... If a label other than `Chr` is desirable, it can be configured with the `synteny_plotsr_seq_label` parameter. + ## Minimum System Requirements All the modules have been tested to work on a single machine with 10 CPUs + 32 GBs of memory, except NCBI FCS GX and Kraken2. Their minimum requirements are: diff --git a/nextflow.config b/nextflow.config index b313f4ac..0e7c24bb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -29,22 +29,22 @@ params { contamination_stops_pipeline = true + // tidk options + tidk_skip = true + tidk_repeat_seq = null + tidk_filter_by_size = false + tidk_filter_size_bp = 1000000 + // BUSCO options busco_skip = true busco_mode = null busco_lineage_datasets = null busco_download_path = null - // TIDK options - tidk_skip = true - tidk_repeat_seq = null - tidk_filter_by_size = false - tidk_filter_size_bp = 1000000 - // LAI options lai_skip = true - // kraken2 options + // kraken 2 options kraken2_skip = true kraken2_db_path = null @@ -55,6 +55,10 @@ params { hic_fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' hic_samtools_ext_args = '-F 3852' + // Merqury options + merqury_skip = true + merqury_kmer_length = 21 + // Synteny options synteny_skip = true synteny_mummer_skip = true @@ -70,11 +74,7 @@ params { synteny_plotsr_seq_label = 'Chr' synteny_plotsr_assembly_order = null - // Merqury options - merqury_skip = true - merqury_kmer_length = 21 - - // Orthofinder options + // OrthoFinder options orthofinder_skip = true // Output options diff --git a/nextflow_schema.json b/nextflow_schema.json index 164e8c9b..a457f73f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -113,6 +113,37 @@ } } }, + "tidk_options": { + "title": "tidk options", + "type": "object", + "description": "", + "default": "", + "properties": { + "tidk_skip": { + "type": "boolean", + "description": "Skip telomere identification", + "default": true, + "fa_icon": "fas fa-forward" + }, + "tidk_repeat_seq": { + "type": "string", + "description": "Telomere repeat sequence. Typical values for plant: TTTAGGG, fungus, vertebrates: TTAGGG and Insect: TTAGG", + "pattern": "^[ACGT]+$", + "fa_icon": "fas fa-dna" + }, + "tidk_filter_by_size": { + "type": "boolean", + "description": "Filter assembly sequences smaller than the specified length", + "fa_icon": "fas fa-question-circle" + }, + "tidk_filter_size_bp": { + "type": "integer", + "default": 1000000, + "description": "Filter size in base-pairs", + "fa_icon": "fas fa-ruler-horizontal" + } + } + }, "busco_options": { "title": "BUSCO options", "type": "object", @@ -145,37 +176,6 @@ } } }, - "tidk_options": { - "title": "TIDK options", - "type": "object", - "description": "", - "default": "", - "properties": { - "tidk_skip": { - "type": "boolean", - "description": "Skip telomere identification", - "default": true, - "fa_icon": "fas fa-forward" - }, - "tidk_repeat_seq": { - "type": "string", - "description": "Telomere repeat sequence. Typical values for plant: TTTAGGG, fungus, vertebrates: TTAGGG and Insect: TTAGG", - "pattern": "^[ACGT]+$", - "fa_icon": "fas fa-dna" - }, - "tidk_filter_by_size": { - "type": "boolean", - "description": "Filter assembly sequences smaller than the specified length", - "fa_icon": "fas fa-question-circle" - }, - "tidk_filter_size_bp": { - "type": "integer", - "default": 1000000, - "description": "Filter size in base-pairs", - "fa_icon": "fas fa-ruler-horizontal" - } - } - }, "lai_options": { "title": "LAI options", "type": "object", @@ -191,7 +191,7 @@ } }, "kraken2_options": { - "title": "Kraken2 options", + "title": "Kraken 2 options", "type": "object", "description": "", "default": "", @@ -246,6 +246,27 @@ } } }, + "merqury_options": { + "title": "Merqury options", + "type": "object", + "description": "", + "default": "", + "properties": { + "merqury_skip": { + "type": "boolean", + "default": true, + "description": "Skip merqury analysis", + "fa_icon": "fas fa-forward" + }, + "merqury_kmer_length": { + "type": "integer", + "default": 21, + "description": "kmer length for merqury analysis", + "minimum": 3, + "fa_icon": "fas fa-ruler-horizontal" + } + } + }, "synteny_options": { "title": "Synteny options", "type": "object", @@ -333,29 +354,8 @@ } } }, - "merqury_options": { - "title": "Merqury options", - "type": "object", - "description": "", - "default": "", - "properties": { - "merqury_skip": { - "type": "boolean", - "default": true, - "description": "Skip merqury analysis", - "fa_icon": "fas fa-forward" - }, - "merqury_kmer_length": { - "type": "integer", - "default": 21, - "description": "kmer length for merqury analysis", - "minimum": 3, - "fa_icon": "fas fa-ruler-horizontal" - } - } - }, "orthofinder_options": { - "title": "Orthofinder options", + "title": "OrthoFinder options", "type": "object", "description": "", "default": "", @@ -466,10 +466,10 @@ "$ref": "#/$defs/ncbi_fcs_options" }, { - "$ref": "#/$defs/busco_options" + "$ref": "#/$defs/tidk_options" }, { - "$ref": "#/$defs/tidk_options" + "$ref": "#/$defs/busco_options" }, { "$ref": "#/$defs/lai_options" @@ -481,10 +481,10 @@ "$ref": "#/$defs/hic_options" }, { - "$ref": "#/$defs/synteny_options" + "$ref": "#/$defs/merqury_options" }, { - "$ref": "#/$defs/merqury_options" + "$ref": "#/$defs/synteny_options" }, { "$ref": "#/$defs/orthofinder_options" From 4f8222e94450d7656fe0b52781d3364e92e584ab Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 13:22:02 +1300 Subject: [PATCH 50/62] Fixed a couple of typos --- README.md | 6 +++--- tests/nextflow.config | 2 +- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 0b4c71e7..aa63b68d 100644 --- a/README.md +++ b/README.md @@ -12,7 +12,7 @@ ## Introduction -**plant-food-research-open/assemblyqc** is a [Nextflow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their references are listed in [CITATIONS.md](./CITATIONS.md). The pipeline includes skip flags to disable execution of many of it tools. +**plant-food-research-open/assemblyqc** is a [Nextflow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their references are listed in [CITATIONS.md](./CITATIONS.md). The pipeline includes skip flags to disable execution of various tools. ## Pipeline Flowchart @@ -29,7 +29,7 @@ - [Kraken 2](https://github.com/DerrickWood/kraken2), [Krona](https://github.com/marbl/Krona): Taxonomy classification - `Alignment and visualisation of HiC data` - [sra-tools](https://github.com/ncbi/sra-tools): HiC data download from SRA or use of local FASTQ files - - [fastp](https://github.com/OpenGene/fastp), [FastQC](https://github.com/s-andrews/FastQC): QC and trimming + - [fastp](https://github.com/OpenGene/fastp), [FastQC](https://github.com/s-andrews/FastQC): Read QC and trimming - [SeqKit sort](https://github.com/shenwei356/seqkit): Alphanumeric sorting of FASTA by sequence ID - [bwa-mem](https://github.com/lh3/bwa): HiC read alignment - [samblaster](https://github.com/GregoryFaust/samblaster): Duplicate marking @@ -43,7 +43,7 @@ - [Merqury](https://github.com/marbl/merqury): Completeness, consensus quality and phasing assessment - `Synteny analysis` - [MUMmer](https://github.com/mummer4/mummer) → [Circos](http://circos.ca/documentation/) + [dotplot](https://plotly.com): One-to-all and all-to-all synteny analysis at the contig level - - [Minimap2](https://github.com/lh3/minimap2) → [Syri](https://github.com/schneebergerlab/syri)/[Plotsr](https://github.com/schneebergerlab/plotsr): One-one to chromosome synteny analysis at the chromosome level + - [Minimap2](https://github.com/lh3/minimap2) → [Syri](https://github.com/schneebergerlab/syri)/[Plotsr](https://github.com/schneebergerlab/plotsr): One-to-one synteny analysis at the chromosome level - `Annotation` - [GenomeTools gt gff3validator](https://genometools.org/tools/gt_gff3validator.html) + [FASTA/GFF correspondence](subworkflows/gallvp/gff3_gt_gff3_gff3validator_stat/main.nf): GFF3 validation - [GenomeTools gt stat](https://genometools.org/tools/gt_stat.html): Annotation statistics diff --git a/tests/nextflow.config b/tests/nextflow.config index 1d5de542..ed1a8053 100644 --- a/tests/nextflow.config +++ b/tests/nextflow.config @@ -5,7 +5,7 @@ */ params { - modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' + modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/' } timeline { enabled = false } From abd8f513523d5444c9f4dc5670c58ac6adc87539 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 13:58:34 +1300 Subject: [PATCH 51/62] Moved around test_full options --- conf/test_full.config | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 818e1c86..9ac77972 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -33,13 +33,13 @@ params { ncbi_fcs_gx_tax_id = 35717 // ncbi_fcs_gx_db_path = 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2023-01-24' + tidk_skip = false + tidk_repeat_seq = 'TTTGGG' + busco_skip = false busco_mode = 'genome' busco_lineage_datasets = 'fungi_odb10 hypocreales_odb10' - tidk_skip = false - tidk_repeat_seq = 'TTTGGG' - lai_skip = false kraken2_skip = true // Skipping this step as the dataset is humengous (126 GB). Please download the dataset manually @@ -47,10 +47,10 @@ params { hic = 'SRR8238190' + merqury_skip = false + synteny_skip = false synteny_mummer_skip = false synteny_plotsr_skip = false synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' - - merqury_skip = false } From c7998d66923e683601f738908f0e6918519e4e4a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 14:12:25 +1300 Subject: [PATCH 52/62] Updated pfr params --- nextflow.config | 8 +++----- pfr/params.json | 17 ++++++++++------- 2 files changed, 13 insertions(+), 12 deletions(-) diff --git a/nextflow.config b/nextflow.config index 0e7c24bb..6541924c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,8 +9,10 @@ // Global default params, used in configs params { - // Input options + // Input/output options input = null + outdir = null + email = null // Validation options check_sequence_duplicates = true @@ -77,10 +79,6 @@ params { // OrthoFinder options orthofinder_skip = true - // Output options - outdir = null - email = null - // Boilerplate options publish_dir_mode = 'copy' email_on_fail = null diff --git a/pfr/params.json b/pfr/params.json index aabf5fba..5d99f9e5 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -1,5 +1,7 @@ { "input": "/workspace/assemblyqc/testdata/v2/assemblysheet.csv", + "outdir": "./results", + "email": null, "check_sequence_duplicates": true, "assemblathon_stats_n_limit": 100, "gfastats_skip": false, @@ -9,14 +11,14 @@ "ncbi_fcs_gx_tax_id": 3750, "ncbi_fcs_gx_db_path": "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24", "contamination_stops_pipeline": false, - "busco_skip": false, - "busco_mode": "genome", - "busco_lineage_datasets": "embryophyta_odb10 eudicots_odb10", - "busco_download_path": "/workspace/ComparativeDataSources/BUSCO/assemblyqc", "tidk_skip": false, "tidk_repeat_seq": "TTTAGGG", "tidk_filter_by_size": true, "tidk_filter_size_bp": 1000000, + "busco_skip": false, + "busco_mode": "genome", + "busco_lineage_datasets": "embryophyta_odb10 eudicots_odb10", + "busco_download_path": "/workspace/ComparativeDataSources/BUSCO/assemblyqc", "lai_skip": false, "kraken2_skip": false, "kraken2_db_path": "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314", @@ -24,6 +26,9 @@ "hic_skip_fastp": false, "hic_skip_fastqc": false, "hic_fastp_ext_args": "--qualified_quality_phred 20 --length_required 50", + "hic_samtools_ext_args": "-F 3852", + "merqury_skip": false, + "merqury_kmer_length": 21, "synteny_skip": false, "synteny_mummer_skip": false, "synteny_plotsr_skip": false, @@ -37,7 +42,5 @@ "synteny_color_by_contig": true, "synteny_plotsr_seq_label": "Chr", "synteny_plotsr_assembly_order": "gddh13_v1p1 m9_v1 m9_v1_h1 m9_v1_h2", - "merqury_skip": false, - "outdir": "./results", - "email": null + "orthofinder_skip": false } From 8fcc5ce1929177448ce9064127469b481cc7dcc5 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 14:22:54 +1300 Subject: [PATCH 53/62] Updated pfr_assembly launch script --- conf/test_full.config | 2 +- pfr_assemblyqc | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 9ac77972..5f9580e4 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -43,7 +43,7 @@ params { lai_skip = false kraken2_skip = true // Skipping this step as the dataset is humengous (126 GB). Please download the dataset manually - // kraken2_db_path = 'https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz' + // kraken2_db_path = 'https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240904.tar.gz' hic = 'SRR8238190' diff --git a/pfr_assemblyqc b/pfr_assemblyqc index 62c34a3f..52ad7dee 100644 --- a/pfr_assemblyqc +++ b/pfr_assemblyqc @@ -27,7 +27,7 @@ shift $((OPTIND -1)) ml unload perl ml apptainer/1.1 -ml nextflow/23.04.4 +ml nextflow/24.04.3 export TMPDIR="/workspace/$USER/tmp" export APPTAINER_BINDPATH="$APPTAINER_BINDPATH,$TMPDIR:$TMPDIR,$TMPDIR:/tmp" From cf11c1bd93b4437413078ce3c4b2f94ab5ed0d6a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 20:13:58 +1300 Subject: [PATCH 54/62] Version bump and a couple of minor fixes --- .github/version_checks.sh | 2 ++ CHANGELOG.md | 2 +- CITATION.cff | 2 +- modules/local/generatekaryotype.nf | 7 ++----- nextflow.config | 2 +- 5 files changed, 7 insertions(+), 8 deletions(-) diff --git a/.github/version_checks.sh b/.github/version_checks.sh index e7b1a90a..3acfa8d6 100755 --- a/.github/version_checks.sh +++ b/.github/version_checks.sh @@ -1,5 +1,7 @@ #!/usr/bin/env bash +set -euo pipefail + config_version=$(sed -n "/^\s*version\s*=\s*'/s/version//p" nextflow.config | tr -d "=[:space:]'") cff_version=$(sed -n '/^version: /s/version: //p' CITATION.cff | tr -d '[:space:]') diff --git a/CHANGELOG.md b/CHANGELOG.md index 5203d463..9a9f8205 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0dev - [04-Nov-2024] +## v2.2.0 - [04-Nov-2024] ### `Added` diff --git a/CITATION.cff b/CITATION.cff index 37a9c841..18bb4b7d 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -25,7 +25,7 @@ authors: - family-names: "Deng" given-names: "Cecilia" title: "AssemblyQC: A Nextflow pipeline for reproducible reporting of assembly quality" -version: 2.2.0dev +version: 2.2.0 date-released: 2024-07-30 url: "https://github.com/Plant-Food-Research-Open/assemblyqc" doi: 10.1093/bioinformatics/btae477 diff --git a/modules/local/generatekaryotype.nf b/modules/local/generatekaryotype.nf index e3771fe6..94cd4f64 100644 --- a/modules/local/generatekaryotype.nf +++ b/modules/local/generatekaryotype.nf @@ -35,8 +35,7 @@ process GENERATEKARYOTYPE { exit 0 fi - tmp_file=\$(mktemp) - printf '%s\\n' "\${ref_seqs[@]}" > "\$tmp_file" + printf '%s\\n' "\${ref_seqs[@]}" > ${target_on_ref}.${seq_tag}.tmp if [[ $seq_tag = "all" ]];then cat $target_seq_len > filtered.target.seq.len @@ -45,7 +44,7 @@ process GENERATEKARYOTYPE { fi cat filtered.target.seq.len | awk '{print \$1,\$2,"grey"}' OFS="\\t" > colored.filtered.target.seq.len - grep -w -f "\$tmp_file" $ref_seq_len > filtered.ref.seq.len + grep -w -f ${target_on_ref}.${seq_tag}.tmp $ref_seq_len > filtered.ref.seq.len cat filtered.ref.seq.len | awk '{print \$1,\$2,"black"}' OFS="\\t" > colored.filtered.ref.seq.len cat colored.filtered.ref.seq.len | sort -k1V > merged.seq.lengths @@ -67,8 +66,6 @@ process GENERATEKARYOTYPE { | sed '/^\$/d' \ | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \ > karyotype_target.tsv - - rm "\$tmp_file" """ stub: diff --git a/nextflow.config b/nextflow.config index 6541924c..0677fb0d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -274,7 +274,7 @@ manifest { description = """A Nextflow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=24.04.2' - version = '2.2.0dev' + version = '2.2.0' doi = 'https://doi.org/10.1093/bioinformatics/btae477' } From af5c449b3c65fca7dd0c9ddf0dde4ea48e86b832 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 21:57:06 +1300 Subject: [PATCH 55/62] Updated PFR's Kraken 2 database to k2_pluspfp_20240904 --- CHANGELOG.md | 1 + docs/usage.md | 2 +- pfr/params.json | 2 +- pfr_assemblyqc | 2 +- 4 files changed, 4 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9a9f8205..e05122f4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 10. Updated the tube map along with the tool list [#166](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/166) 11. Added Orthofinder [#167](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/167) 12. Changed order of tool options in the `nextflow.config` file +13. Updated PFR's Kraken 2 database to `k2_pluspfp_20240904` [#170](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/170) ### `Fixed` diff --git a/docs/usage.md b/docs/usage.md index 02b198b3..dd38f31f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -65,7 +65,7 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p ### Kraken 2 -Path to Kraken 2 database is provided by the `kraken2_db_path` parameter. This can be a URL to a public `.tar.gz` file such as `https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz`. The pipeline can download and extract the database. This is not the recommended practice owing to the size of the database. Rather, the database should be downloaded, extracted and stored in a read-only location. The path to that location can be provided by the `kraken2_db_path` parameter such as `/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314`. +Path to Kraken 2 database is provided by the `kraken2_db_path` parameter. This can be a URL to a public `.tar.gz` file such as `https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz`. The pipeline can download and extract the database. This is not the recommended practice owing to the size of the database. Rather, the database should be downloaded, extracted and stored in a read-only location. The path to that location can be provided by the `kraken2_db_path` parameter such as `/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20240904`. ## Other parameters diff --git a/pfr/params.json b/pfr/params.json index 5d99f9e5..8b4f0625 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -21,7 +21,7 @@ "busco_download_path": "/workspace/ComparativeDataSources/BUSCO/assemblyqc", "lai_skip": false, "kraken2_skip": false, - "kraken2_db_path": "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314", + "kraken2_db_path": "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20240904", "hic": null, "hic_skip_fastp": false, "hic_skip_fastqc": false, diff --git a/pfr_assemblyqc b/pfr_assemblyqc index 52ad7dee..b860ae42 100644 --- a/pfr_assemblyqc +++ b/pfr_assemblyqc @@ -40,7 +40,7 @@ if [ $full_test_flag -eq 1 ]; then --ncbi_fcs_gx_skip false \ --ncbi_fcs_gx_db_path "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" \ --kraken2_skip false \ - --kraken2_db_path "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" \ + --kraken2_db_path "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20240904" \ -resume \ --outdir results else From e14050942418575723fe6fa1199d17acf9ab971c Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 11:29:50 +1300 Subject: [PATCH 56/62] Increased memory requirement for Kraken 2 to 256.GB --- CHANGELOG.md | 3 ++- conf/base.config | 2 +- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index e05122f4..dec4217f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0 - [04-Nov-2024] +## v2.2.0 - [05-Nov-2024] ### `Added` @@ -20,6 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 11. Added Orthofinder [#167](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/167) 12. Changed order of tool options in the `nextflow.config` file 13. Updated PFR's Kraken 2 database to `k2_pluspfp_20240904` [#170](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/170) +14. Increased memory requirement for Kraken 2 to `256.GB` ### `Fixed` diff --git a/conf/base.config b/conf/base.config index f13b4759..e39fbece 100644 --- a/conf/base.config +++ b/conf/base.config @@ -61,7 +61,7 @@ process { memory = { 512.GB * task.attempt } } withName:KRAKEN2 { - memory = { 200.GB * task.attempt } + memory = { 256.GB * task.attempt } } withName:BWA_MEM { time = { 2.day * task.attempt } From d98384a2266b026feffc0a06abdde3534c15743b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 11:56:12 +1300 Subject: [PATCH 57/62] Updated docs and removed resource limits from test_full --- conf/test_full.config | 8 -------- docs/usage.md | 2 +- local_assemblyqc | 1 + 3 files changed, 2 insertions(+), 9 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 5f9580e4..f2ad3d1a 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -10,14 +10,6 @@ ---------------------------------------------------------------------------------------- */ -process { - resourceLimits = [ - cpus: 10, - memory: '32.GB', - time: '6.h' - ] -} - params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' diff --git a/docs/usage.md b/docs/usage.md index dd38f31f..3090d3c5 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -129,7 +129,7 @@ The data for these examples comes from: [umd.edu](https://obj.umiacs.umd.edu/mar All the modules have been tested to work on a single machine with 10 CPUs + 32 GBs of memory, except NCBI FCS GX and Kraken2. Their minimum requirements are: - NCBI FCS GX: 1 CPU + 512 GBs memory -- Kraken2: 1 CPU + 200 GBs memory +- Kraken2: 1 CPU + 250 GBs memory ## Running the pipeline diff --git a/local_assemblyqc b/local_assemblyqc index bcfff8c3..3a211c34 100755 --- a/local_assemblyqc +++ b/local_assemblyqc @@ -17,6 +17,7 @@ nextflow run \ -profile docker,test_full \ -resume \ $stub \ + -c ../nxf-config/resources.config \ --ncbi_fcs_gx_skip false \ --ncbi_fcs_gx_db_path ../dbs/gxdb/test \ --busco_download_path ../dbs/busco \ From 0da7cea5f4a0d04af7ba4a5ce536fb6a30532250 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 12:07:31 +1300 Subject: [PATCH 58/62] Updated synteny_mummer_min_bundle_size for pfr/params --- pfr/params.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/pfr/params.json b/pfr/params.json index 8b4f0625..a4062925 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -37,7 +37,7 @@ "synteny_mummer_plot_type": "both", "synteny_mummer_m2m_align": false, "synteny_mummer_max_gap": 1000000, - "synteny_mummer_min_bundle_size": 1000, + "synteny_mummer_min_bundle_size": 1000000, "synteny_plot_1_vs_all": false, "synteny_color_by_contig": true, "synteny_plotsr_seq_label": "Chr", From e6b968fe0df22fac2f9cdba05d6d0fc2a8003a55 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 16:41:04 +1300 Subject: [PATCH 59/62] Now using _JAVA_OPTIONS in module RUNASSEMBLYVISUALIZER to avoid user preferences related errors --- CHANGELOG.md | 1 + modules/local/runassemblyvisualizer.nf | 10 ++-------- 2 files changed, 3 insertions(+), 8 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index dec4217f..1c5def16 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 1. Fixed a bug where Gene score distribution graph did not appear correctly [#125](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/125) 2. Increased memory requirement for `DNADIFF` to avoid SLURM OOM kills with exit code 2 [#141](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/141) 3. Documented the use explicit use of `-revision` parameter [#160](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/160) +4. Now using `_JAVA_OPTIONS` in module `RUNASSEMBLYVISUALIZER` to avoid user preferences related errors ### `Dependencies` diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf index 5452dfd0..d52bc590 100644 --- a/modules/local/runassemblyvisualizer.nf +++ b/modules/local/runassemblyvisualizer.nf @@ -21,14 +21,9 @@ process RUNASSEMBLYVISUALIZER { assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') file_name="${agp_assembly_file}" - cp -r /usr/src/3d-dna/ \\ - 3d-dna + export _JAVA_OPTIONS="-Djava.util.prefs.userRoot=user_prefs -Xms${avail_mem}g -Xmx${avail_mem}g" - sed -i \\ - 's/-Xms49152m -Xmx49152m/-Xms${avail_mem}g -Xmx${avail_mem}g/1' \\ - 3d-dna/visualize/juicebox_tools.sh - - 3d-dna/visualize/run-assembly-visualizer.sh \\ + /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\ -p false \\ $agp_assembly_file $sorted_links_txt_file @@ -42,7 +37,6 @@ process RUNASSEMBLYVISUALIZER { stub: if ( !task.memory ) { error '[RUNASSEMBLYVISUALIZER] Available memory not known. Specify process memory requirements to fix this.' } - def avail_mem = (task.memory.giga*0.8).intValue() """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') touch "\${assembly_tag}.hic" From 0f9a4eebbf39ae4dda11227ad38db38afdcebca2 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 17:37:36 +1300 Subject: [PATCH 60/62] Now added user.home property to JAVA_OPTIONS --- modules/local/runassemblyvisualizer.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf index d52bc590..e4ca50a4 100644 --- a/modules/local/runassemblyvisualizer.nf +++ b/modules/local/runassemblyvisualizer.nf @@ -21,7 +21,8 @@ process RUNASSEMBLYVISUALIZER { assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') file_name="${agp_assembly_file}" - export _JAVA_OPTIONS="-Djava.util.prefs.userRoot=user_prefs -Xms${avail_mem}g -Xmx${avail_mem}g" + mkdir user_home + export _JAVA_OPTIONS="-Djava.util.prefs.userRoot=user_prefs -Duser.home=user_home -Xms${avail_mem}g -Xmx${avail_mem}g" /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\ -p false \\ From ddc9aae8d9612d5aed6173defc00f23a48632048 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 20:44:26 +1300 Subject: [PATCH 61/62] Fixed version mismatches --- docs/usage.md | 2 +- tests/README.md | 2 +- tests/hicparam/main.nf.test.snap | 2 +- tests/invalid/main.nf.test.snap | 2 +- tests/minimal/main.nf.test.snap | 2 +- tests/noltr/main.nf.test.snap | 2 +- tests/orthofinder/main.nf.test.snap | 4 ++-- tests/stub/main.nf.test.snap | 2 +- tests/tiny/main.nf.test.snap | 2 +- 9 files changed, 10 insertions(+), 10 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 3090d3c5..8590c615 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -129,7 +129,7 @@ The data for these examples comes from: [umd.edu](https://obj.umiacs.umd.edu/mar All the modules have been tested to work on a single machine with 10 CPUs + 32 GBs of memory, except NCBI FCS GX and Kraken2. Their minimum requirements are: - NCBI FCS GX: 1 CPU + 512 GBs memory -- Kraken2: 1 CPU + 250 GBs memory +- Kraken2: 1 CPU + 256 GBs memory ## Running the pipeline diff --git a/tests/README.md b/tests/README.md index 6f09f846..a9af91a8 100644 --- a/tests/README.md +++ b/tests/README.md @@ -20,7 +20,7 @@ The GitHub [CI action](../.github/workflows/ci.yml) included with the pipeline c ## Testing with a Large Dataset at Plant&Food -Before each release, the functionality of the entire pipeline is tested with a large dataset on the on-prem SLURM-based HPC at The New Zealand Institute of Plant and Food Research. +Before each release, the functionality of the entire pipeline is tested with a large dataset on the on-prem SLURM-based HPC at The New Zealand Institute for Plant and Food Research. ## Testing Merqury Datasets diff --git a/tests/hicparam/main.nf.test.snap b/tests/hicparam/main.nf.test.snap index feebbdbc..db89beee 100644 --- a/tests/hicparam/main.nf.test.snap +++ b/tests/hicparam/main.nf.test.snap @@ -68,7 +68,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ diff --git a/tests/invalid/main.nf.test.snap b/tests/invalid/main.nf.test.snap index 9aa72f46..c29b15ad 100644 --- a/tests/invalid/main.nf.test.snap +++ b/tests/invalid/main.nf.test.snap @@ -35,7 +35,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ diff --git a/tests/minimal/main.nf.test.snap b/tests/minimal/main.nf.test.snap index 44a5d9fc..8e139a7e 100644 --- a/tests/minimal/main.nf.test.snap +++ b/tests/minimal/main.nf.test.snap @@ -35,7 +35,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ diff --git a/tests/noltr/main.nf.test.snap b/tests/noltr/main.nf.test.snap index 75ee9f5f..d1580b9d 100644 --- a/tests/noltr/main.nf.test.snap +++ b/tests/noltr/main.nf.test.snap @@ -50,7 +50,7 @@ "seqkit": "v2.8.0" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ diff --git a/tests/orthofinder/main.nf.test.snap b/tests/orthofinder/main.nf.test.snap index 633b6c30..4d34b485 100644 --- a/tests/orthofinder/main.nf.test.snap +++ b/tests/orthofinder/main.nf.test.snap @@ -41,7 +41,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ @@ -80,4 +80,4 @@ }, "timestamp": "2024-11-01T14:11:21.865104" } -} \ No newline at end of file +} diff --git a/tests/stub/main.nf.test.snap b/tests/stub/main.nf.test.snap index 74b3740e..d2e05d4c 100644 --- a/tests/stub/main.nf.test.snap +++ b/tests/stub/main.nf.test.snap @@ -230,7 +230,7 @@ "untar": 1.34 }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ diff --git a/tests/tiny/main.nf.test.snap b/tests/tiny/main.nf.test.snap index 60dfdbbe..b937e1d4 100644 --- a/tests/tiny/main.nf.test.snap +++ b/tests/tiny/main.nf.test.snap @@ -29,7 +29,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0dev" + "plant-food-research-open/assemblyqc": "v2.2.0" } }, "stable paths": [ From ab0f094a0c1925199072d0c34d4cdc6a39e757f4 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 5 Nov 2024 21:18:22 +1300 Subject: [PATCH 62/62] Excluded included_configs from linting --- .nf-core.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.nf-core.yml b/.nf-core.yml index 902fd78b..b62355dd 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -3,6 +3,7 @@ lint: actions_ci: false multiqc_config: false template_strings: false + included_configs: false files_exist: - conf/igenomes.config - conf/igenomes_ignored.config
diff --git a/docs/usage.md b/docs/usage.md index 9b9c7ebd..f29a8082 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -83,9 +83,9 @@ The above pipeline run specified with a params file in yaml format: nextflow run plant-food-research-open/assemblyqc -profile docker -params-file params.yaml ``` -with `params.yaml` containing: +with: -```yaml +```yaml title="params.yaml" input: './samplesheet.csv' outdir: './results/' genome: 'GRCh37' @@ -108,7 +108,7 @@ It is a good idea to specify a pipeline version when running the pipeline on you First, go to the [plant-food-research-open/assemblyqc releases page](https://github.com/plant-food-research-open/assemblyqc/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. -This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. @@ -197,14 +197,6 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -## Azure Resource Requests - -To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. -We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required. - -Note that the choice of VM size depends on your quota and the overall workload during the analysis. -For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes). - ## Running in the background Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. diff --git a/main.nf b/main.nf index e417dccf..c8a0cc72 100644 --- a/main.nf +++ b/main.nf @@ -7,8 +7,6 @@ ---------------------------------------------------------------------------------------- */ -nextflow.enable.dsl = 2 - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS @@ -18,20 +16,6 @@ nextflow.enable.dsl = 2 include { ASSEMBLYQC } from './workflows/assemblyqc' include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline' include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline' - -include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_assemblyqc_pipeline' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = getGenomeAttribute('fasta') - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOWS FOR PIPELINE @@ -54,10 +38,6 @@ workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC { ASSEMBLYQC ( samplesheet ) - - emit: - multiqc_report = ASSEMBLYQC.out.multiqc_report // channel: /path/to/multiqc_report.html - } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -68,27 +48,24 @@ workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC { workflow { main: - // // SUBWORKFLOW: Run initialisation tasks // PIPELINE_INITIALISATION ( params.version, - params.help, params.validate_params, params.monochrome_logs, args, params.outdir, params.input ) - + // // WORKFLOW: Run main workflow // PLANTFOODRESEARCHOPEN_ASSEMBLYQC ( PIPELINE_INITIALISATION.out.samplesheet ) - // // SUBWORKFLOW: Run completion tasks // @@ -99,7 +76,7 @@ workflow { params.outdir, params.monochrome_logs, params.hook_url, - PLANTFOODRESEARCHOPEN_ASSEMBLYQC.out.multiqc_report + ) } diff --git a/modules.json b/modules.json index 8558f6d2..5f5fbb68 100644 --- a/modules.json +++ b/modules.json @@ -4,34 +4,23 @@ "repos": { "https://github.com/nf-core/modules.git": { "modules": { - "nf-core": { - "fastqc": { - "branch": "master", - "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": ["modules"] - }, - "multiqc": { - "branch": "master", - "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] - } - } + "nf-core": {} }, "subworkflows": { "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "d20fb2a9cc3e2835e9d067d1046a63252eb17352", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "git_sha": "2fdce49d30c0254f76bc0f13c55c17455c1251ab", "installed_by": ["subworkflows"] }, - "utils_nfvalidation_plugin": { + "utils_nfschema_plugin": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml deleted file mode 100644 index 1787b38a..00000000 --- a/modules/nf-core/fastqc/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: fastqc -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index d79f1c86..00000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,61 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') - // FastQC memory value allowed range (100 - 10000) - def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) - - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - --memory $fastqc_memory \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index ee5507e0..00000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index 70edae4d..00000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,212 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("sarscov2 single-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
Mon 2 Oct 2023
test.gz
- // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } - ) - } - } - - test("sarscov2 paired-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } - ) - } - } - - test("sarscov2 interleaved [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } - ) - } - } - - test("sarscov2 paired-end [bam]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } - ) - } - } - - test("sarscov2 multiple [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } - ) - } - } - - test("sarscov2 custom_prefix") { - - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } - ) - } - } - - test("sarscov2 single-end [fastq] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } - ) - } - } - -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index 86f7c311..00000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,88 +0,0 @@ -{ - "fastqc_versions_interleaved": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:07.293713" - }, - "fastqc_stub": { - "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:31:01.425198" - }, - "fastqc_versions_multiple": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:55.797907" - }, - "fastqc_versions_bam": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:26.795862" - }, - "fastqc_versions_single": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:27.043675" - }, - "fastqc_versions_paired": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:47.584191" - }, - "fastqc_versions_custom_prefix": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:41:14.576531" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294b..00000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml deleted file mode 100644 index ca39fb67..00000000 --- a/modules/nf-core/multiqc/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: multiqc -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf deleted file mode 100644 index 47ac352f..00000000 --- a/modules/nf-core/multiqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process MULTIQC { - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : - 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" - - input: - path multiqc_files, stageAs: "?/*" - path(multiqc_config) - path(extra_multiqc_config) - path(multiqc_logo) - - output: - path "*multiqc_report.html", emit: report - path "*_data" , emit: data - path "*_plots" , optional:true, emit: plots - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def config = multiqc_config ? "--config $multiqc_config" : '' - def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' - def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' - """ - multiqc \\ - --force \\ - $args \\ - $config \\ - $extra_config \\ - $logo \\ - . - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ - - stub: - """ - mkdir multiqc_data - touch multiqc_plots - touch multiqc_report.html - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml deleted file mode 100644 index 45a9bc35..00000000 --- a/modules/nf-core/multiqc/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: multiqc -description: Aggregate results from bioinformatics analyses across many samples into a single report -keywords: - - QC - - bioinformatics tools - - Beautiful stand-alone HTML report -tools: - - multiqc: - description: | - MultiQC searches a given directory for analysis logs and compiles a HTML report. - It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. - homepage: https://multiqc.info/ - documentation: https://multiqc.info/docs/ - licence: ["GPL-3.0-or-later"] -input: - - multiqc_files: - type: file - description: | - List of reports / files recognised by MultiQC, for example the html and zip output of FastQC - - multiqc_config: - type: file - description: Optional config yml for MultiQC - pattern: "*.{yml,yaml}" - - extra_multiqc_config: - type: file - description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. - pattern: "*.{yml,yaml}" - - multiqc_logo: - type: file - description: Optional logo file for MultiQC - pattern: "*.{png}" -output: - - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" - - data: - type: directory - description: MultiQC data dir - pattern: "multiqc_data" - - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@abhi18av" - - "@bunop" - - "@drpatelh" - - "@jfy133" -maintainers: - - "@abhi18av" - - "@bunop" - - "@drpatelh" - - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test deleted file mode 100644 index f1c4242e..00000000 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ /dev/null @@ -1,84 +0,0 @@ -nextflow_process { - - name "Test Process MULTIQC" - script "../main.nf" - process "MULTIQC" - - tag "modules" - tag "modules_nfcore" - tag "multiqc" - - test("sarscov2 single-end [fastqc]") { - - when { - process { - """ - input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, - { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("multiqc_versions_single") } - ) - } - - } - - test("sarscov2 single-end [fastqc] [config]") { - - when { - process { - """ - input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) - input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, - { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("multiqc_versions_config") } - ) - } - } - - test("sarscov2 single-end [fastqc] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.report.collect { file(it).getName() } + - process.out.data.collect { file(it).getName() } + - process.out.plots.collect { file(it).getName() } + - process.out.versions ).match("multiqc_stub") } - ) - } - - } -} diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap deleted file mode 100644 index bfebd802..00000000 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ /dev/null @@ -1,41 +0,0 @@ -{ - "multiqc_versions_single": { - "content": [ - [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-29T08:48:55.657331" - }, - "multiqc_stub": { - "content": [ - [ - "multiqc_report.html", - "multiqc_data", - "multiqc_plots", - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-29T08:49:49.071937" - }, - "multiqc_versions_config": { - "content": [ - [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-29T08:49:25.457567" - } -} \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml deleted file mode 100644 index bea6c0d3..00000000 --- a/modules/nf-core/multiqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -multiqc: - - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 6bae5f67..7cb553f5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,17 +12,6 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null - // References - genome = null - igenomes_base = 's3://ngi-igenomes/igenomes/' - igenomes_ignore = false - - // MultiQC options - multiqc_config = null - multiqc_title = null - multiqc_logo = null - max_multiqc_email_size = '25.MB' - multiqc_methods_description = null // Boilerplate options outdir = null @@ -33,48 +22,26 @@ params { monochrome_logs = false hook_url = null help = false + help_full = false + show_hidden = false version = false pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' - // Config options config_profile_name = null config_profile_description = null + custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - - // Max resource options - // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' - // Schema validation default options - validationFailUnrecognisedParams = false - validationLenientMode = false - validationSchemaIgnoreParams = 'genomes,igenomes_base' - validationShowHiddenParams = false - validate_params = true - + validate_params = true + } // Load base.config by default for all pipelines includeConfig 'conf/base.config' -// Load nf-core custom profiles from different Institutions -try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") -} - -// Load plant-food-research-open/assemblyqc custom profiles from different institutions. -try { - includeConfig "${params.custom_config_base}/pipeline/assemblyqc.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config/assemblyqc profiles: ${params.custom_config_base}/pipeline/assemblyqc.config") -} profiles { debug { dumpHashes = true @@ -89,7 +56,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false - conda.channels = ['conda-forge', 'bioconda', 'defaults'] + conda.channels = ['conda-forge', 'bioconda'] apptainer.enabled = false } mamba { @@ -178,25 +145,21 @@ profiles { test_full { includeConfig 'conf/test_full.config' } } -// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile -// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled -// Set to your registry if you have a mirror of containers -apptainer.registry = 'quay.io' -docker.registry = 'quay.io' -podman.registry = 'quay.io' -singularity.registry = 'quay.io' +// Load nf-core custom profiles from different Institutions +includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" -// Nextflow plugins -plugins { - id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet -} +// Load plant-food-research-open/assemblyqc custom profiles from different institutions. +// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs +// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/assemblyqc.config" : "/dev/null" +// Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile +// Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled +// Set to your registry if you have a mirror of containers +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' +charliecloud.registry = 'quay.io' -// Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} else { - params.genomes = [:] -} // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -208,8 +171,15 @@ env { JULIA_DEPOT_PATH = "/usr/local/share/julia" } -// Capture exit codes from upstream processes when piping -process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Set bash options +process.shell = """\ +bash + +set -e # Exit if a tool returns a non-zero status/exit code +set -u # Treat unset variables and parameters as an error +set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute +set -C # No clobber - prevent output redirection from overwriting files. +""" // Disable process selector warnings by default. Use debug profile to enable warnings. nextflow.enable.configProcessNamesValidation = false @@ -238,43 +208,26 @@ manifest { homePage = 'https://github.com/plant-food-research-open/assemblyqc' description = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' - nextflowVersion = '!>=23.04.0' - version = '2.0.0+dev' + nextflowVersion = '!>=24.04.2' + version = '1.0.0dev' doi = '' } -// Load modules.config for DSL2 module specific options -includeConfig 'conf/modules.config' +// Nextflow plugins +plugins { + id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} -// Function to ensure that resource requirements don't go beyond -// a maximum limit -def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } +validation { + defaultIgnoreParams = ["genomes"] + help { + enabled = true + command = "nextflow run $manifest.name -profile --input samplesheet.csv --outdir " + fullParameter = "help_full" + showHiddenParameter = "show_hidden" } } + +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + diff --git a/nextflow_schema.json b/nextflow_schema.json index 971d65a2..fe2c1d96 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,10 +1,10 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/nextflow_schema.json", "title": "plant-food-research-open/assemblyqc pipeline parameters", "description": "A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.", "type": "object", - "definitions": { + "$defs": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -35,42 +35,6 @@ "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." - }, - "fasta": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." } } }, @@ -122,41 +86,6 @@ } } }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, "generic_options": { "title": "Generic options", "type": "object", @@ -164,12 +93,6 @@ "description": "Less common options for the pipeline, typically set in a config file.", "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, "version": { "type": "boolean", "description": "Display version and exit.", @@ -199,14 +122,6 @@ "fa_icon": "fas fa-remove-format", "hidden": true }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, "monochrome_logs": { "type": "boolean", "description": "Do not use coloured log outputs.", @@ -220,24 +135,6 @@ "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", "hidden": true }, - "multiqc_config": { - "type": "string", - "format": "file-path", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, "validate_params": { "type": "boolean", "description": "Boolean whether to validate parameters against the schema at runtime", @@ -245,27 +142,6 @@ "fa_icon": "fas fa-check-square", "hidden": true }, - "validationShowHiddenParams": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "validationFailUnrecognisedParams": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters fails when an unrecognised parameter is found.", - "hidden": true, - "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." - }, - "validationLenientMode": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters in lenient more.", - "hidden": true, - "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." - }, "pipelines_testdata_base_path": { "type": "string", "fa_icon": "far fa-check-circle", @@ -278,19 +154,14 @@ }, "allOf": [ { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/reference_genome_options" - }, - { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/$defs/input_output_options" }, + { - "$ref": "#/definitions/max_job_request_options" + "$ref": "#/$defs/institutional_config_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/$defs/generic_options" } ] } diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf index e8fa98a5..fdc1719f 100644 --- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf @@ -8,17 +8,14 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' -include { paramsSummaryMap } from 'plugin/nf-validation' -include { fromSamplesheet } from 'plugin/nf-validation' -include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { UTILS_NFSCHEMA_PLUGIN } from '../../nf-core/utils_nfschema_plugin' +include { paramsSummaryMap } from 'plugin/nf-schema' +include { samplesheetToList } from 'plugin/nf-schema' include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' -include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' -include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' include { imNotification } from '../../nf-core/utils_nfcore_pipeline' include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' -include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' /* ======================================================================================== @@ -30,7 +27,6 @@ workflow PIPELINE_INITIALISATION { take: version // boolean: Display version and exit - help // boolean: Display help text validate_params // boolean: Boolean whether to validate parameters against the schema at runtime monochrome_logs // boolean: Do not use coloured log outputs nextflow_cli_args // array: List of positional nextflow CLI args @@ -51,20 +47,16 @@ workflow PIPELINE_INITIALISATION { workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 ) + // // Validate parameters and generate parameter summary to stdout // - pre_help_text = nfCoreLogo(monochrome_logs) - post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) - def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " - UTILS_NFVALIDATION_PLUGIN ( - help, - workflow_command, - pre_help_text, - post_help_text, + UTILS_NFSCHEMA_PLUGIN ( + workflow, validate_params, - "nextflow_schema.json" + null ) + // // Check config provided to the pipeline @@ -72,16 +64,13 @@ workflow PIPELINE_INITIALISATION { UTILS_NFCORE_PIPELINE ( nextflow_cli_args ) - // - // Custom validation for pipeline parameters - // - validateInputParameters() // // Create channel from input file provided through params.input // + Channel - .fromSamplesheet("input") + .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json")) .map { meta, fastq_1, fastq_2 -> if (!fastq_2) { @@ -91,8 +80,8 @@ workflow PIPELINE_INITIALISATION { } } .groupTuple() - .map { - validateInputSamplesheet(it) + .map { samplesheet -> + validateInputSamplesheet(samplesheet) } .map { meta, fastqs -> @@ -117,13 +106,13 @@ workflow PIPELINE_COMPLETION { email // string: email address email_on_fail // string: email address sent on pipeline failure plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published monochrome_logs // boolean: Disable ANSI colour codes in log output hook_url // string: hook URL for notifications - multiqc_report // string: Path to MultiQC report + main: - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") // @@ -131,11 +120,18 @@ workflow PIPELINE_COMPLETION { // workflow.onComplete { if (email || email_on_fail) { - completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + completionEmail( + summary_params, + email, + email_on_fail, + plaintext_email, + outdir, + monochrome_logs, + [] + ) } completionSummary(monochrome_logs) - if (hook_url) { imNotification(summary_params, hook_url) } @@ -151,12 +147,6 @@ workflow PIPELINE_COMPLETION { FUNCTIONS ======================================================================================== */ -// -// Check and validate pipeline parameters -// -def validateInputParameters() { - genomeExistsError() -} // // Validate channels from input samplesheet @@ -165,39 +155,13 @@ def validateInputSamplesheet(input) { def (metas, fastqs) = input[1..2] // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end - def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1 if (!endedness_ok) { error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") } return [ metas[0], fastqs ] } -// -// Get attribute from genome config file e.g. fasta -// -def getGenomeAttribute(attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null -} - -// -// Exit pipeline if incorrect --genome key provided -// -def genomeExistsError() { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - error(error_string) - } -} - // // Generate methods description for MultiQC // @@ -207,8 +171,8 @@ def toolCitationText() { // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", + + "." ].join(' ').trim() @@ -220,8 +184,8 @@ def toolBibliographyText() { // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
  • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
  • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
  • " + + ].join(' ').trim() return reference_text @@ -239,8 +203,10 @@ def methodsDescriptionText(mqc_methods_yaml) { // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list def temp_doi_ref = "" - String[] manifest_doi = meta.manifest_map.doi.tokenize(",") - for (String doi_ref: manifest_doi) temp_doi_ref += "(doi:
    ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + def manifest_doi = meta.manifest_map.doi.tokenize(",") + manifest_doi.each { doi_ref -> + temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + } meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) } else meta["doi_text"] = "" meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
  • " @@ -261,3 +227,4 @@ def methodsDescriptionText(mqc_methods_yaml) { return description_html.toString() } + diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index ac31f28f..28e32b20 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -2,10 +2,6 @@ // Subworkflow with functionality that may be useful for any Nextflow pipeline // -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - /* ======================================================================================== SUBWORKFLOW DEFINITION @@ -58,7 +54,7 @@ workflow UTILS_NEXTFLOW_PIPELINE { // Generate version string // def getWorkflowVersion() { - String version_string = "" + def version_string = "" as String if (workflow.manifest.version) { def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' version_string += "${prefix_v}${workflow.manifest.version}" @@ -79,10 +75,10 @@ def dumpParametersToJSON(outdir) { def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') def filename = "params_${timestamp}.json" def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) + def jsonStr = groovy.json.JsonOutput.toJson(params) + temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) - FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") temp_pf.delete() } @@ -90,7 +86,7 @@ def dumpParametersToJSON(outdir) { // When running with -profile conda, warn if channels have not been set-up appropriately // def checkCondaChannels() { - Yaml parser = new Yaml() + def parser = new org.yaml.snakeyaml.Yaml() def channels = [] try { def config = parser.load("conda config --show channels".execute().text) @@ -102,14 +98,16 @@ def checkCondaChannels() { // Check that all channels are present // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def required_channels_in_order = ['conda-forge', 'bioconda'] def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + + required_channels_in_order.eachWithIndex { channel, index -> + if (index < required_channels_in_order.size() - 1) { + channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index+1])) + } } if (channels_missing | channel_priority_violation) { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index d0a926bf..a09572e5 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index 14558c39..cbd8495b 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -2,9 +2,6 @@ // Subworkflow with utility functions specific to the nf-core pipeline template // -import org.yaml.snakeyaml.Yaml -import nextflow.extension.FilesEx - /* ======================================================================================== SUBWORKFLOW DEFINITION @@ -34,7 +31,7 @@ workflow UTILS_NFCORE_PIPELINE { // Warn if a -profile or Nextflow config has not been provided to run the pipeline // def checkConfigProvided() { - valid_config = true + def valid_config = true as Boolean if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + @@ -66,11 +63,13 @@ def checkProfileProvided(nextflow_cli_args) { // def workflowCitation() { def temp_doi_ref = "" - String[] manifest_doi = workflow.manifest.doi.tokenize(",") + def manifest_doi = workflow.manifest.doi.tokenize(",") // Using a loop to handle multiple DOIs // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list - for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + manifest_doi.each { doi_ref -> + temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + } return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + @@ -84,7 +83,7 @@ def workflowCitation() { // Generate workflow version string // def getWorkflowVersion() { - String version_string = "" + def version_string = "" as String if (workflow.manifest.version) { def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' version_string += "${prefix_v}${workflow.manifest.version}" @@ -102,8 +101,8 @@ def getWorkflowVersion() { // Get software versions for pipeline // def processVersionsFromYAML(yaml_file) { - Yaml yaml = new Yaml() - versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + def yaml = new org.yaml.snakeyaml.Yaml() + def versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } return yaml.dumpAsMap(versions).trim() } @@ -124,7 +123,7 @@ def workflowVersionToYAML() { def softwareVersionsToYAML(ch_versions) { return ch_versions .unique() - .map { processVersionsFromYAML(it) } + .map { version -> processVersionsFromYAML(version) } .unique() .mix(Channel.of(workflowVersionToYAML())) } @@ -134,19 +133,19 @@ def softwareVersionsToYAML(ch_versions) { // def paramsSummaryMultiqc(summary_params) { def summary_section = '' - for (group in summary_params.keySet()) { + summary_params.keySet().each { group -> def group_params = summary_params.get(group) // This gets the parameters of that particular group if (group_params) { summary_section += "

    $group

    \n" summary_section += "
    \n" - for (param in group_params.keySet()) { + group_params.keySet().sort().each { param -> summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" } summary_section += "
    \n" } } - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + def yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" as String yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" @@ -161,7 +160,7 @@ def paramsSummaryMultiqc(summary_params) { // nf-core logo // def nfCoreLogo(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map String.format( """\n ${dashedLine(monochrome_logs)} @@ -180,7 +179,7 @@ def nfCoreLogo(monochrome_logs=true) { // Return dashed line // def dashedLine(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map return "-${colors.dim}----------------------------------------------------${colors.reset}-" } @@ -188,7 +187,7 @@ def dashedLine(monochrome_logs=true) { // ANSII colours used for terminal logging // def logColours(monochrome_logs=true) { - Map colorcodes = [:] + def colorcodes = [:] as Map // Reset / Meta colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" @@ -287,7 +286,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi } def summary = [:] - for (group in summary_params.keySet()) { + summary_params.keySet().sort().each { group -> summary << summary_params[group] } @@ -344,10 +343,10 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi def sendmail_html = sendmail_template.toString() // Send the HTML e-mail - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map if (email_address) { try { - if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + if (plaintext_email) { throw new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") sendmail_tf.withWriter { w -> w << sendmail_html } @@ -364,13 +363,13 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Write summary e-mail HTML to a file def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); output_hf.delete() // Write summary e-mail TXT to a file def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); output_tf.delete() } @@ -378,7 +377,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Print pipeline summary on completion // def completionSummary(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map if (workflow.success) { if (workflow.stats.ignoredCount == 0) { log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" @@ -395,7 +394,7 @@ def completionSummary(monochrome_logs=true) { // def imNotification(summary_params, hook_url) { def summary = [:] - for (group in summary_params.keySet()) { + summary_params.keySet().sort().each { group -> summary << summary_params[group] } diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf new file mode 100644 index 00000000..4994303e --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf @@ -0,0 +1,46 @@ +// +// Subworkflow that uses the nf-schema plugin to validate parameters and render the parameter summary +// + +include { paramsSummaryLog } from 'plugin/nf-schema' +include { validateParameters } from 'plugin/nf-schema' + +workflow UTILS_NFSCHEMA_PLUGIN { + + take: + input_workflow // workflow: the workflow object used by nf-schema to get metadata from the workflow + validate_params // boolean: validate the parameters + parameters_schema // string: path to the parameters JSON schema. + // this has to be the same as the schema given to `validation.parametersSchema` + // when this input is empty it will automatically use the configured schema or + // "${projectDir}/nextflow_schema.json" as default. This input should not be empty + // for meta pipelines + + main: + + // + // Print parameter summary to stdout. This will display the parameters + // that differ from the default given in the JSON schema + // + if(parameters_schema) { + log.info paramsSummaryLog(input_workflow, parameters_schema:parameters_schema) + } else { + log.info paramsSummaryLog(input_workflow) + } + + // + // Validate the parameters using nextflow_schema.json or the schema + // given via the validation.parametersSchema configuration option + // + if(validate_params) { + if(parameters_schema) { + validateParameters(parameters_schema:parameters_schema) + } else { + validateParameters() + } + } + + emit: + dummy_emit = true +} + diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml new file mode 100644 index 00000000..f7d9f028 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml @@ -0,0 +1,35 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "utils_nfschema_plugin" +description: Run nf-schema to validate parameters and create a summary of changed parameters +keywords: + - validation + - JSON schema + - plugin + - parameters + - summary +components: [] +input: + - input_workflow: + type: object + description: | + The workflow object of the used pipeline. + This object contains meta data used to create the params summary log + - validate_params: + type: boolean + description: Validate the parameters and error if invalid. + - parameters_schema: + type: string + description: | + Path to the parameters JSON schema. + This has to be the same as the schema given to the `validation.parametersSchema` config + option. When this input is empty it will automatically use the configured schema or + "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way + for meta pipelines. +output: + - dummy_emit: + type: boolean + description: Dummy emit to make nf-core subworkflows lint happy +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test new file mode 100644 index 00000000..842dc432 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test @@ -0,0 +1,117 @@ +nextflow_workflow { + + name "Test Subworkflow UTILS_NFSCHEMA_PLUGIN" + script "../main.nf" + workflow "UTILS_NFSCHEMA_PLUGIN" + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/utils_nfschema_plugin" + tag "plugin/nf-schema" + + config "./nextflow.config" + + test("Should run nothing") { + + when { + + params { + test_data = '' + } + + workflow { + """ + validate_params = false + input[0] = workflow + input[1] = validate_params + input[2] = "" + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should validate params") { + + when { + + params { + test_data = '' + outdir = 1 + } + + workflow { + """ + validate_params = true + input[0] = workflow + input[1] = validate_params + input[2] = "" + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } } + ) + } + } + + test("Should run nothing - custom schema") { + + when { + + params { + test_data = '' + } + + workflow { + """ + validate_params = false + input[0] = workflow + input[1] = validate_params + input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should validate params - custom schema") { + + when { + + params { + test_data = '' + outdir = 1 + } + + workflow { + """ + validate_params = true + input[0] = workflow + input[1] = validate_params + input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config new file mode 100644 index 00000000..0907ac58 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config @@ -0,0 +1,8 @@ +plugins { + id "nf-schema@2.1.0" +} + +validation { + parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + monochromeLogs = true +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json similarity index 95% rename from subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json rename to subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json index 7626c1c9..331e0d2f 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json @@ -1,10 +1,10 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", "title": ". pipeline parameters", "description": "", "type": "object", - "definitions": { + "$defs": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -87,10 +87,10 @@ }, "allOf": [ { - "$ref": "#/definitions/input_output_options" + "$ref": "#/$defs/input_output_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/$defs/generic_options" } ] } diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf deleted file mode 100644 index 2585b65d..00000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -// -// Subworkflow that uses the nf-validation plugin to render help text and parameter summary -// - -/* -======================================================================================== - IMPORT NF-VALIDATION PLUGIN -======================================================================================== -*/ - -include { paramsHelp } from 'plugin/nf-validation' -include { paramsSummaryLog } from 'plugin/nf-validation' -include { validateParameters } from 'plugin/nf-validation' - -/* -======================================================================================== - SUBWORKFLOW DEFINITION -======================================================================================== -*/ - -workflow UTILS_NFVALIDATION_PLUGIN { - - take: - print_help // boolean: print help - workflow_command // string: default commmand used to run pipeline - pre_help_text // string: string to be printed before help text and summary log - post_help_text // string: string to be printed after help text and summary log - validate_params // boolean: validate parameters - schema_filename // path: JSON schema file, null to use default value - - main: - - log.debug "Using schema file: ${schema_filename}" - - // Default values for strings - pre_help_text = pre_help_text ?: '' - post_help_text = post_help_text ?: '' - workflow_command = workflow_command ?: '' - - // - // Print help message if needed - // - if (print_help) { - log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text - System.exit(0) - } - - // - // Print parameter summary to stdout - // - log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text - - // - // Validate parameters relative to the parameter JSON schema - // - if (validate_params){ - validateParameters(parameters_schema: schema_filename) - } - - emit: - dummy_emit = true -} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml deleted file mode 100644 index 3d4a6b04..00000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml +++ /dev/null @@ -1,44 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "UTILS_NFVALIDATION_PLUGIN" -description: Use nf-validation to initiate and validate a pipeline -keywords: - - utility - - pipeline - - initialise - - validation -components: [] -input: - - print_help: - type: boolean - description: | - Print help message and exit - - workflow_command: - type: string - description: | - The command to run the workflow e.g. "nextflow run main.nf" - - pre_help_text: - type: string - description: | - Text to print before the help message - - post_help_text: - type: string - description: | - Text to print after the help message - - validate_params: - type: boolean - description: | - Validate the parameters and error if invalid. - - schema_filename: - type: string - description: | - The filename of the schema to validate against. -output: - - dummy_emit: - type: boolean - description: | - Dummy emit to make nf-core subworkflows lint happy -authors: - - "@adamrtalbot" -maintainers: - - "@adamrtalbot" - - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test deleted file mode 100644 index 5784a33f..00000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ /dev/null @@ -1,200 +0,0 @@ -nextflow_workflow { - - name "Test Workflow UTILS_NFVALIDATION_PLUGIN" - script "../main.nf" - workflow "UTILS_NFVALIDATION_PLUGIN" - tag "subworkflows" - tag "subworkflows_nfcore" - tag "plugin/nf-validation" - tag "'plugin/nf-validation'" - tag "utils_nfvalidation_plugin" - tag "subworkflows/utils_nfvalidation_plugin" - - test("Should run nothing") { - - when { - - params { - monochrome_logs = true - test_data = '' - } - - workflow { - """ - help = false - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success } - ) - } - } - - test("Should run help") { - - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } } - ) - } - } - - test("Should run help with command") { - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = "nextflow run noorg/doesntexist" - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } } - ) - } - } - - test("Should run help with extra text") { - - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = "nextflow run noorg/doesntexist" - pre_help_text = "pre-help-text" - post_help_text = "post-help-text" - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('pre-help-text') } }, - { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } }, - { assert workflow.stdout.any { it.contains('post-help-text') } } - ) - } - } - - test("Should validate params") { - - when { - - params { - monochrome_logs = true - test_data = '' - outdir = 1 - } - workflow { - """ - help = false - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = true - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.failed }, - { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } - ) - } - } -} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml deleted file mode 100644 index 60b1cfff..00000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/utils_nfvalidation_plugin: - - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/tower.yml b/tower.yml index 787aedfe..c61323c0 100644 --- a/tower.yml +++ b/tower.yml @@ -1,5 +1,3 @@ reports: - multiqc_report.html: - display: "MultiQC HTML report" samplesheet.csv: display: "Auto-created samplesheet with collated metadata and FASTQ paths" diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index b47addae..61922ddb 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -4,10 +4,9 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-validation' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' + +include { paramsSummaryMap } from 'plugin/nf-schema' + include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_assemblyqc_pipeline' @@ -21,20 +20,10 @@ workflow ASSEMBLYQC { take: ch_samplesheet // channel: samplesheet read in from --input - main: ch_versions = Channel.empty() - ch_multiqc_files = Channel.empty() - - // - // MODULE: Run FastQC - // - FASTQC ( - ch_samplesheet - ) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + // // Collate and save software versions @@ -42,53 +31,15 @@ workflow ASSEMBLYQC { softwareVersionsToYAML(ch_versions) .collectFile( storeDir: "${params.outdir}/pipeline_info", - name: 'nf_core_pipeline_software_mqc_versions.yml', + name: '' + 'pipeline_software_' + '' + 'versions.yml', sort: true, newLine: true ).set { ch_collated_versions } - // - // MODULE: MultiQC - // - ch_multiqc_config = Channel.fromPath( - "$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? - Channel.fromPath(params.multiqc_config, checkIfExists: true) : - Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? - Channel.fromPath(params.multiqc_logo, checkIfExists: true) : - Channel.empty() - - summary_params = paramsSummaryMap( - workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? - file(params.multiqc_methods_description, checkIfExists: true) : - file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value( - methodsDescriptionText(ch_multiqc_custom_methods_description)) - - ch_multiqc_files = ch_multiqc_files.mix( - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix( - ch_methods_description.collectFile( - name: 'methods_description_mqc.yaml', - sort: true - ) - ) - - MULTIQC ( - ch_multiqc_files.collect(), - ch_multiqc_config.toList(), - ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() - ) emit: - multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html versions = ch_versions // channel: [ path(versions.yml) ] + } /* From 9fc79890f07bab6a655eeaf77c6bab2989284da2 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 18:22:48 +1300 Subject: [PATCH 08/62] Updated nf-core/template to 3.0.0 --- CHANGELOG.md | 3 ++- nextflow_schema.json | 1 + 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 436cacf8..5967d72a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,11 +3,12 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0+dev - [01-Oct-2024] +## v2.2.0+dev - [09-Oct-2024] ### `Added` 1. Added Gfastats [#126](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/126) +2. Updated nf-core/template to 3.0.0 [#149](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/149) ### `Fixed` diff --git a/nextflow_schema.json b/nextflow_schema.json index 54954f39..433b8dd1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -582,3 +582,4 @@ } ] } +} From c81f941de23919255ac8bd950cc0a190e25d17a7 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 18:33:59 +1300 Subject: [PATCH 09/62] Fixed schema issues --- nextflow_schema.json | 134 +++++++++++++++++++------------------------ 1 file changed, 60 insertions(+), 74 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 433b8dd1..f414edbf 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -35,10 +35,7 @@ "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" } }, - "required": [ - "input", - "outdir" - ] + "required": ["input", "outdir"] }, "validation_options": { "title": "Validation options", @@ -88,10 +85,7 @@ }, "ncbi_fcs_adaptor_empire": { "type": "string", - "enum": [ - "euk", - "prok" - ], + "enum": ["euk", "prok"], "description": "Empire for NCBI FCS Adaptor checking: 'euk' for eukaryotes, or 'prok' for prokaryotes", "fa_icon": "fas fa-project-diagram" }, @@ -134,11 +128,7 @@ }, "busco_mode": { "type": "string", - "enum": [ - "genome", - "transcriptome", - "proteins" - ], + "enum": ["genome", "transcriptome", "proteins"], "description": "BUSCO mode: 'genome', 'transcriptome', 'proteins'", "fa_icon": "fas fa-cog" }, @@ -293,11 +283,7 @@ "type": "string", "default": "both", "description": "Synteny plot type from Mummer alignments: 'dotplot', 'circos', or 'both'", - "enum": [ - "both", - "dotplot", - "circos" - ], + "enum": ["both", "dotplot", "circos"], "fa_icon": "fas fa-chart-line" }, "synteny_mummer_m2m_align": { @@ -460,14 +446,7 @@ "default": "copy", "description": "Method used to save pipeline results to output directory.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { @@ -531,55 +510,62 @@ "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", "hidden": true } + }, + "validationSchemaIgnoreParams": { + "type": "string" + }, + "pipelines_testdata_base_path": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/" } } + } + }, + "allOf": [ + { + "$ref": "#/$defs/input_output_options" }, - "allOf": [ - { - "$ref": "#/$defs/input_output_options" - }, - { - "$ref": "#/$defs/institutional_config_options" - }, - { - "$ref": "#/defs/validation_options" - }, - { - "$ref": "#/defs/general_stats_options" - }, - { - "$ref": "#/defs/ncbi_fcs_options" - }, - { - "$ref": "#/defs/busco_options" - }, - { - "$ref": "#/defs/tidk_options" - }, - { - "$ref": "#/defs/lai_options" - }, - { - "$ref": "#/defs/kraken2_options" - }, - { - "$ref": "#/defs/hic_options" - }, - { - "$ref": "#/defs/synteny_options" - }, - { - "$ref": "#/defs/merqury_options" - }, - { - "$ref": "#/defs/max_job_request_options" - }, - { - "$ref": "#/defs/institutional_config_options" - }, - { - "$ref": "#/$defs/generic_options" - } - ] - } + { + "$ref": "#/$defs/institutional_config_options" + }, + { + "$ref": "#/$defs/validation_options" + }, + { + "$ref": "#/$defs/general_stats_options" + }, + { + "$ref": "#/$defs/ncbi_fcs_options" + }, + { + "$ref": "#/$defs/busco_options" + }, + { + "$ref": "#/$defs/tidk_options" + }, + { + "$ref": "#/$defs/lai_options" + }, + { + "$ref": "#/$defs/kraken2_options" + }, + { + "$ref": "#/$defs/hic_options" + }, + { + "$ref": "#/$defs/synteny_options" + }, + { + "$ref": "#/$defs/merqury_options" + }, + { + "$ref": "#/$defs/max_job_request_options" + }, + { + "$ref": "#/$defs/institutional_config_options" + }, + { + "$ref": "#/$defs/generic_options" + } + ] } From 2dfe3687aad7632b8d5f245fa878ce9e857ea967 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 18:57:08 +1300 Subject: [PATCH 10/62] Removed unnecessary params --- CHANGELOG.md | 2 ++ docs/parameters.md | 6 ----- nextflow.config | 13 +---------- nextflow_schema.json | 52 +------------------------------------------- 4 files changed, 4 insertions(+), 69 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5967d72a..1bc0b181 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -21,6 +21,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 1. Reduced the GenomeTools stats figures to 300 DPI [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) 2. Now `synteny_mummer_min_bundle_size` is set to `1000000` by default [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) +3. `results` is not the default output directory anymore +4. Removed a number of unnecessary parameters: `monochromeLogs`, `config_profile_contact`, `config_profile_url`, `validationFailUnrecognisedParams`, `validationLenientMode`, `validationSchemaIgnoreParams`, `validationShowHiddenParams` ## v2.1.1 - [20-Sep-2024] diff --git a/docs/parameters.md b/docs/parameters.md index c9d7eefb..8e0867e8 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -119,8 +119,6 @@ Parameters used to describe centralised config profiles. These should not be edi | `custom_config_base` | Base directory for Institutional configs. | `string` | https://raw.githubusercontent.com/nf-core/configs/master | | True | | `config_profile_name` | Institutional config name. | `string` | | | True | | `config_profile_description` | Institutional config description. | `string` | | | True | -| `config_profile_contact` | Institutional config contact information. | `string` | | | True | -| `config_profile_url` | Institutional config URL link. | `string` | | | True | ## Generic options @@ -134,9 +132,5 @@ Less common options for the pipeline, typically set in a config file. | `email_on_fail` | Email address for completion summary, only when pipeline fails. | `string` | | | True | | `plaintext_email` | Send plain-text email instead of HTML. | `boolean` | | | True | | `monochrome_logs` | Do not use coloured log outputs. | `boolean` | | | True | -| `monochromeLogs` | Do not use coloured log outputs. | `boolean` | | | True | | `hook_url` | Incoming hook URL for messaging service | `string` | | | True | | `validate_params` | Boolean whether to validate parameters against the schema at runtime | `boolean` | True | | True | -| `validationShowHiddenParams` | Show all params when using `--help` | `boolean` | | | True | -| `validationFailUnrecognisedParams` | Validation of parameters fails when an unrecognised parameter is found. | `boolean` | | | True | -| `validationLenientMode` | Validation of parameters in lenient more. | `boolean` | | | True | diff --git a/nextflow.config b/nextflow.config index 05a1c922..e22eeb07 100644 --- a/nextflow.config +++ b/nextflow.config @@ -74,7 +74,7 @@ params { merqury_kmer_length = 21 // Output options - outdir = './results' + outdir = null email = null // Max resource options @@ -87,27 +87,16 @@ params { email_on_fail = null plaintext_email = false monochrome_logs = false - monochromeLogs = false hook_url = null - help = false - help_full = false - show_hidden = false version = false - pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null config_profile_description = null custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - config_profile_contact = null - config_profile_url = null // Schema validation default options - validationFailUnrecognisedParams = false - validationLenientMode = false - validationSchemaIgnoreParams = '' - validationShowHiddenParams = false validate_params = true } diff --git a/nextflow_schema.json b/nextflow_schema.json index f414edbf..986db2ea 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -25,8 +25,7 @@ "type": "string", "format": "directory-path", "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open", - "default": "./results" + "fa_icon": "fas fa-folder-open" }, "email": { "type": "string", @@ -413,18 +412,6 @@ "description": "Institutional config description.", "hidden": true, "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" } } }, @@ -468,12 +455,6 @@ "fa_icon": "fas fa-palette", "hidden": true }, - "monochromeLogs": { - "type": "boolean", - "fa_icon": "fas fa-palette", - "description": "Do not use coloured log outputs.", - "hidden": true - }, "hook_url": { "type": "string", "description": "Incoming hook URL for messaging service", @@ -486,37 +467,6 @@ "default": true, "fa_icon": "fas fa-check-square", "hidden": true - }, - "validationShowHiddenParams": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true - }, - "validationFailUnrecognisedParams": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters fails when an unrecognised parameter is found.", - "hidden": true - }, - "validationLenientMode": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters in lenient more.", - "pipelines_testdata_base_path": { - "type": "string", - "fa_icon": "far fa-check-circle", - "description": "Base URL or local path to location of pipeline test dataset files", - "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", - "hidden": true - } - }, - "validationSchemaIgnoreParams": { - "type": "string" - }, - "pipelines_testdata_base_path": { - "type": "string", - "default": "https://raw.githubusercontent.com/nf-core/test-datasets/" } } } From 8817696d387c2ff4229a86fe42cea099b5e78408 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 19:21:56 +1300 Subject: [PATCH 11/62] Removed max resource params --- .nf-core.yml | 17 +------------ CHANGELOG.md | 1 + nextflow.config | 15 +++++++----- nextflow_schema.json | 35 --------------------------- tests/hicparam/params.json | 5 +--- tests/invalid/params.json | 5 +--- tests/merqury/mixed2x/params.json | 5 +--- tests/merqury/phased2x.mp/params.json | 5 +--- tests/merqury/phased2x/params.json | 5 +--- tests/minimal/params.json | 5 +--- tests/noltr/params.json | 5 +--- tests/stub/params.json | 5 +--- 12 files changed, 19 insertions(+), 89 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 8fcc88a4..a6433ac8 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,9 +1,6 @@ bump_version: null lint: files_exist: - - conf/igenomes.config - - conf/igenomes_ignored.config - - assets/multiqc_config.yml - conf/igenomes.config - conf/igenomes_ignored.config - assets/multiqc_config.yml @@ -14,18 +11,6 @@ lint: - .github/ISSUE_TEMPLATE/config.yml - .github/workflows/awstest.yml - .github/workflows/awsfulltest.yml - - assets/multiqc_config.yml - - conf/igenomes.config - files_unchanged: - - .github/CONTRIBUTING.md - - assets/sendmail_template.txt - - .github/CONTRIBUTING.md - - assets/sendmail_template.txt - - CODE_OF_CONDUCT.md - - assets/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_dark.png - - .github/ISSUE_TEMPLATE/bug_report.yml multiqc_config: false template_strings: false nextflow_config: @@ -51,5 +36,5 @@ template: - igenomes - multiqc - fastqc - version: 1.0.0dev + version: 2.2.0+dev update: null diff --git a/CHANGELOG.md b/CHANGELOG.md index 1bc0b181..5b9bd0db 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -23,6 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 2. Now `synteny_mummer_min_bundle_size` is set to `1000000` by default [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) 3. `results` is not the default output directory anymore 4. Removed a number of unnecessary parameters: `monochromeLogs`, `config_profile_contact`, `config_profile_url`, `validationFailUnrecognisedParams`, `validationLenientMode`, `validationSchemaIgnoreParams`, `validationShowHiddenParams` +5. Resource parameters have been removed: `max_memory`, `max_cpus`, `max_time` ## v2.1.1 - [20-Sep-2024] diff --git a/nextflow.config b/nextflow.config index e22eeb07..ea8342eb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -77,11 +77,6 @@ params { outdir = null email = null - // Max resource options - max_memory = '512.GB' - max_cpus = 16 - max_time = '7.day' - // Boilerplate options publish_dir_mode = 'copy' email_on_fail = null @@ -101,6 +96,15 @@ params { } +// // Max resources +process { + resourceLimits = [ + cpus: 16, + memory: '512.GB', + time: '7.day' + ] +} + // Load base.config by default for all pipelines includeConfig 'conf/base.config' @@ -212,7 +216,6 @@ profiles { includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" // Load plant-food-research-open/assemblyqc custom profiles from different institutions. -// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs // includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/assemblyqc.config" : "/dev/null" // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled diff --git a/nextflow_schema.json b/nextflow_schema.json index 986db2ea..082ba205 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -348,38 +348,6 @@ } } }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job. Example: '8.GB'", - "default": "512.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job. Example: '1.day'", - "default": "7.day", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true - } - } - }, "institutional_config_options": { "title": "Institutional config options", "type": "object", @@ -508,9 +476,6 @@ { "$ref": "#/$defs/merqury_options" }, - { - "$ref": "#/$defs/max_job_request_options" - }, { "$ref": "#/$defs/institutional_config_options" }, diff --git a/tests/hicparam/params.json b/tests/hicparam/params.json index 2f0c2d51..c99d6c4b 100644 --- a/tests/hicparam/params.json +++ b/tests/hicparam/params.json @@ -2,8 +2,5 @@ "config_profile_name": "Test to verify hic param validation", "config_profile_description": "Test to verify hic param validation", "input": "tests/hicparam/assemblysheet.csv", - "hic": "tests/hicparam/hic/Dummy_hic_{1,2}.merged.fq.gz", - "max_cpus": 2, - "max_memory": "6.GB", - "max_time": "6.h" + "hic": "tests/hicparam/hic/Dummy_hic_{1,2}.merged.fq.gz" } diff --git a/tests/invalid/params.json b/tests/invalid/params.json index dd017c16..eb2dab01 100644 --- a/tests/invalid/params.json +++ b/tests/invalid/params.json @@ -1,8 +1,5 @@ { "config_profile_name": "Invalid profile", "config_profile_description": "Profile to test invalid files", - "input": "tests/invalid/assemblysheet.csv", - "max_cpus": 2, - "max_memory": "6.GB", - "max_time": "6.h" + "input": "tests/invalid/assemblysheet.csv" } diff --git a/tests/merqury/mixed2x/params.json b/tests/merqury/mixed2x/params.json index 6ce89d4b..aa96496d 100644 --- a/tests/merqury/mixed2x/params.json +++ b/tests/merqury/mixed2x/params.json @@ -3,8 +3,5 @@ "config_profile_description": "Merqury test for a mixed diploid assembly contained in a single fasta", "input": "tests/merqury/mixed2x/assemblysheet.csv", "merqury_skip": false, - "merqury_kmer_length": 21, - "max_cpus": 8, - "max_memory": "32.GB", - "max_time": "6.h" + "merqury_kmer_length": 21 } diff --git a/tests/merqury/phased2x.mp/params.json b/tests/merqury/phased2x.mp/params.json index 60f157ca..0b4479f0 100644 --- a/tests/merqury/phased2x.mp/params.json +++ b/tests/merqury/phased2x.mp/params.json @@ -3,8 +3,5 @@ "config_profile_description": "Merqury test for a phased diploid assembly contained in separate fasta with parental reads", "input": "tests/merqury/phased2x.mp/assemblysheet.csv", "merqury_skip": false, - "merqury_kmer_length": 21, - "max_cpus": 8, - "max_memory": "32.GB", - "max_time": "6.h" + "merqury_kmer_length": 21 } diff --git a/tests/merqury/phased2x/params.json b/tests/merqury/phased2x/params.json index 0b363fab..e37db9ab 100644 --- a/tests/merqury/phased2x/params.json +++ b/tests/merqury/phased2x/params.json @@ -3,8 +3,5 @@ "config_profile_description": "Merqury test for a phased diploid assembly contained in separate fasta", "input": "tests/merqury/phased2x/assemblysheet.csv", "merqury_skip": false, - "merqury_kmer_length": 21, - "max_cpus": 8, - "max_memory": "32.GB", - "max_time": "6.h" + "merqury_kmer_length": 21 } diff --git a/tests/minimal/params.json b/tests/minimal/params.json index c5683622..0b8c5117 100644 --- a/tests/minimal/params.json +++ b/tests/minimal/params.json @@ -1,8 +1,5 @@ { "config_profile_name": "Test profile", "config_profile_description": "Minimal test dataset to check pipeline function", - "input": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv", - "max_cpus": 2, - "max_memory": "6.GB", - "max_time": "6.h" + "input": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv" } diff --git a/tests/noltr/params.json b/tests/noltr/params.json index a46bd34d..e52470db 100644 --- a/tests/noltr/params.json +++ b/tests/noltr/params.json @@ -2,8 +2,5 @@ "config_profile_name": "No LTRs assembly profile", "config_profile_description": "Profile to test an assembly without LTRs", "input": "tests/noltr/assemblysheet.csv", - "lai_skip": false, - "max_cpus": 2, - "max_memory": "6.GB", - "max_time": "6.h" + "lai_skip": false } diff --git a/tests/stub/params.json b/tests/stub/params.json index 573986a7..85bdf0ce 100644 --- a/tests/stub/params.json +++ b/tests/stub/params.json @@ -23,8 +23,5 @@ "synteny_mummer_skip": false, "synteny_plotsr_skip": false, "synteny_xref_assemblies": "assets/xrefsheet.csv", - "merqury_skip": false, - "max_cpus": 2, - "max_memory": "6.GB", - "max_time": "6.h" + "merqury_skip": false } From 30b8e2e2aa75a97f665a20737412aa642a4a9b37 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 19:28:27 +1300 Subject: [PATCH 12/62] Fixed linting issues --- .nf-core.yml | 7 ++ assets/email_template.html | 136 ++++++++++------------------------- assets/sendmail_template.txt | 22 ++++++ 3 files changed, 66 insertions(+), 99 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index a6433ac8..cb312f8c 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -11,6 +11,13 @@ lint: - .github/ISSUE_TEMPLATE/config.yml - .github/workflows/awstest.yml - .github/workflows/awsfulltest.yml + files_unchanged: + - LICENSE + - .github/CONTRIBUTING.md + - .github/PULL_REQUEST_TEMPLATE.md + - .github/workflows/branch.yml + - .github/workflows/linting.yml + - docs/README.md multiqc_config: false template_strings: false nextflow_config: diff --git a/assets/email_template.html b/assets/email_template.html index e150ec26..672f3f2a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,8 +1,8 @@ - - - - + + + + plant-food-research-open/assemblyqc Pipeline Report @@ -10,106 +10,44 @@
    -

    plant-food-research-open/assemblyqc ${version}

    -

    Run Name: $runName

    + - <% if (!success){ out << """ -
    -

    - plant-food-research-open/assemblyqc execution completed unsuccessfully! -

    +

    plant-food-research-open/assemblyqc ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    plant-food-research-open/assemblyqc execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    -
    ${errorReport}
    -
    - """ } else { out << """ -
    +
    ${errorReport}
    +
    + """ +} else { + out << """ +
    plant-food-research-open/assemblyqc execution completed successfully! -
    - """ } %> +
    + """ +} +%> -

    The workflow was completed at $dateComplete (duration: $duration)

    -

    The command used to launch the workflow was as follows:

    -
    -$commandLine
    +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    -

    Pipeline Configuration:

    - - - <% out << summary.collect{ k,v -> " - - - - - " }.join("\n") %> - -
    - $k - -
    $v
    -
    +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    -

    plant-food-research-open/assemblyqc

    -

    - https://github.com/plant-food-research-open/assemblyqc -

    -
    - +

    plant-food-research-open/assemblyqc

    +

    https://github.com/plant-food-research-open/assemblyqc

    + + + + diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 20644943..0ba5f393 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -26,6 +26,28 @@ Content-Disposition: inline; filename="plant-food-research-open-assemblyqc_logo_ join( '\n' ) %> <% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} %> --nfcoremimeboundary-- From 1404b2935bf60ec9c6155aad814b8a456728e294 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 20:29:45 +1300 Subject: [PATCH 13/62] Migrated to nf-schema@2.1.1 --- CHANGELOG.md | 2 +- assets/schema_input.json | 25 +++++++++++-------- .../utils_nfcore_assemblyqc_pipeline/main.nf | 2 +- 3 files changed, 16 insertions(+), 13 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5b9bd0db..3c54ef0f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,7 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Dependencies` 1. Nextflow!>=23.04.0 -2. nf-validation@1.1.3 +2. nf-schema@2.1.1 ### `Deprecated` diff --git a/assets/schema_input.json b/assets/schema_input.json index beb55ec5..83f4915d 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -6,6 +6,7 @@ "type": "array", "items": { "type": "object", + "uniqueEntries": ["tag"], "properties": { "tag": { "type": "string", @@ -72,8 +73,7 @@ "type": "string", "maxLength": 0 } - ], - "dependentRequired": ["reads_1"] + ] }, "maternal_reads_1": { "errorMessage": "maternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'", @@ -86,8 +86,7 @@ "type": "string", "maxLength": 0 } - ], - "dependentRequired": ["reads_1", "paternal_reads_1"] + ] }, "maternal_reads_2": { "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'", @@ -100,8 +99,7 @@ "type": "string", "maxLength": 0 } - ], - "dependentRequired": ["maternal_reads_1"] + ] }, "paternal_reads_1": { "errorMessage": "paternal_reads_1 should be a SRA ID for paired FASTQ files or FASTX file path without spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'", @@ -114,8 +112,7 @@ "type": "string", "maxLength": 0 } - ], - "dependentRequired": ["reads_1", "maternal_reads_1"] + ] }, "paternal_reads_2": { "errorMessage": "FASTX file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na|astq|q)' or '.f(a|asta|as|sa|na|astq|q).gz'", @@ -128,10 +125,16 @@ "type": "string", "maxLength": 0 } - ], - "dependentRequired": ["paternal_reads_1"] + ] } }, - "required": ["tag", "fasta"] + "required": ["tag", "fasta"], + "dependentRequired": { + "reads_2": ["reads_1"], + "maternal_reads_1": ["reads_1", "paternal_reads_1"], + "maternal_reads_2": ["maternal_reads_1"], + "paternal_reads_1": ["reads_1", "maternal_reads_1"], + "paternal_reads_2": ["paternal_reads_1"] + } } } diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf index 32133c87..061bb380 100644 --- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf @@ -76,7 +76,7 @@ workflow PIPELINE_INITIALISATION { // Initialise input channels // - ch_input = Channel.fromSamplesheet('input') + ch_input = Channel.fromList (samplesheetToList(input, "assets/schema_input.json")) // Function: validateInputTags ch_input_validated = ch_input From 7afc159cb9731e73ac08aad0afdd93a2522c163f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 21:36:51 +1300 Subject: [PATCH 14/62] Fixed test_full issues --- assets/schema_xref_assemblies.json | 3 ++- conf/base.config | 14 +++++++------- conf/test_full.config | 8 ++++++++ docs/usage.md | 2 +- local_assemblyqc | 5 ++--- nextflow.config | 2 +- pfr_assemblyqc | 3 ++- .../local/utils_nfcore_assemblyqc_pipeline/main.nf | 2 +- 8 files changed, 24 insertions(+), 15 deletions(-) diff --git a/assets/schema_xref_assemblies.json b/assets/schema_xref_assemblies.json index 0da6ca1c..9a82cfbf 100644 --- a/assets/schema_xref_assemblies.json +++ b/assets/schema_xref_assemblies.json @@ -1,11 +1,12 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/main/assets/schema_xref_assemblies.json", "title": "plant-food-research-open/assemblyqc pipeline - params.synteny_xref_assemblies schema", "description": "Schema for the file provided with params.synteny_xref_assemblies", "type": "array", "items": { "type": "object", + "uniqueEntries": ["tag"], "properties": { "tag": { "type": "string", diff --git a/conf/base.config b/conf/base.config index d9df1884..20473408 100644 --- a/conf/base.config +++ b/conf/base.config @@ -57,23 +57,23 @@ process { maxRetries = 2 } withName:NCBI_FCS_GX_SCREEN_SAMPLES { - time = { check_max( 20.h * task.attempt, 'time' ) } - memory = { check_max( 512.GB * task.attempt, 'memory' ) } + time = { 20.h * task.attempt } + memory = { 512.GB * task.attempt } } withName:KRAKEN2 { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } + memory = { 200.GB * task.attempt } } withName:BWA_MEM { - time = { check_max( 2.day * task.attempt, 'time' ) } + time = { 2.day * task.attempt } } withName:SAMBLASTER { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { 20.h * task.attempt } } withName:DNADIFF { - time = { check_max( 7.day * task.attempt, 'time' ) } + time = { 7.day * task.attempt } } withName:MERQURY_HAPMERS { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { 20.h * task.attempt } } withName:CREATEREPORT { cache = false diff --git a/conf/test_full.config b/conf/test_full.config index 14f95cb9..2eadbf9b 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -10,6 +10,14 @@ ---------------------------------------------------------------------------------------- */ +process { + resourceLimits = [ + cpus: 10, + memory: '32.GB', + time: '6.h' + ] +} + params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' diff --git a/docs/usage.md b/docs/usage.md index 1a5a994a..20f10823 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -126,7 +126,7 @@ The data for these examples comes from: [umd.edu](https://obj.umiacs.umd.edu/mar ## Minimum System Requirements -All the modules have been tested to work on a single machine with 10 CPUs + 30 GBs of memory, except NCBI FCS GX and Kraken2. Their minimum requirements are: +All the modules have been tested to work on a single machine with 10 CPUs + 32 GBs of memory, except NCBI FCS GX and Kraken2. Their minimum requirements are: - NCBI FCS GX: 1 CPU + 512 GBs memory - Kraken2: 1 CPU + 200 GBs memory diff --git a/local_assemblyqc b/local_assemblyqc index 858acea8..bcfff8c3 100755 --- a/local_assemblyqc +++ b/local_assemblyqc @@ -17,10 +17,9 @@ nextflow run \ -profile docker,test_full \ -resume \ $stub \ - --max_cpus 8 \ - --max_memory '32.GB' \ --ncbi_fcs_gx_skip false \ --ncbi_fcs_gx_db_path ../dbs/gxdb/test \ --busco_download_path ../dbs/busco \ --kraken2_skip false \ - --kraken2_db_path ../dbs/kraken2db/k2_minusb + --kraken2_db_path ../dbs/kraken2db/k2_minusb \ + --outdir results diff --git a/nextflow.config b/nextflow.config index ea8342eb..c002d677 100644 --- a/nextflow.config +++ b/nextflow.config @@ -96,7 +96,7 @@ params { } -// // Max resources +// Max resources process { resourceLimits = [ cpus: 16, diff --git a/pfr_assemblyqc b/pfr_assemblyqc index 8f40d31d..62c34a3f 100644 --- a/pfr_assemblyqc +++ b/pfr_assemblyqc @@ -41,7 +41,8 @@ if [ $full_test_flag -eq 1 ]; then --ncbi_fcs_gx_db_path "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" \ --kraken2_skip false \ --kraken2_db_path "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" \ - -resume + -resume \ + --outdir results else nextflow \ main.nf \ diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf index 061bb380..11bdc8bc 100644 --- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf @@ -101,7 +101,7 @@ workflow PIPELINE_INITIALISATION { ch_xref_assembly = params.synteny_skip || ! params.synteny_xref_assemblies ? Channel.empty() - : Channel.fromSamplesheet('synteny_xref_assemblies') + : Channel.fromList(samplesheetToList(params.synteny_xref_assemblies, "assets/schema_xref_assemblies.json")) ch_xref_assembly_validated = ch_xref_assembly | map { row -> row[0] } From 67db39d40caa7bbb40df03c685ea4e8b0a16eb3f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:00:52 +1300 Subject: [PATCH 15/62] Removed validate_params --- CHANGELOG.md | 2 +- conf/test.config | 4 ++-- docs/parameters.md | 19 +++++++++---------- main.nf | 1 - nextflow.config | 5 +---- nextflow_schema.json | 7 ------- .../utils_nfcore_assemblyqc_pipeline/main.nf | 5 ++--- 7 files changed, 15 insertions(+), 28 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3c54ef0f..b7ff96cb 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -22,7 +22,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 1. Reduced the GenomeTools stats figures to 300 DPI [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) 2. Now `synteny_mummer_min_bundle_size` is set to `1000000` by default [#142](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/142) 3. `results` is not the default output directory anymore -4. Removed a number of unnecessary parameters: `monochromeLogs`, `config_profile_contact`, `config_profile_url`, `validationFailUnrecognisedParams`, `validationLenientMode`, `validationSchemaIgnoreParams`, `validationShowHiddenParams` +4. Removed a number of unnecessary parameters: `monochromeLogs`, `config_profile_contact`, `config_profile_url`, `validationFailUnrecognisedParams`, `validationLenientMode`, `validationSchemaIgnoreParams`, `validationShowHiddenParams` `validate_params` 5. Resource parameters have been removed: `max_memory`, `max_cpus`, `max_time` ## v2.1.1 - [20-Sep-2024] diff --git a/conf/test.config b/conf/test.config index 2fae9b1b..2417719d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -12,8 +12,8 @@ process { resourceLimits = [ - cpus: 2, - memory: '6.GB', + cpus: 4, + memory: '15.GB', time: '1.h' ] } diff --git a/docs/parameters.md b/docs/parameters.md index 8e0867e8..6453ce9b 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -124,13 +124,12 @@ Parameters used to describe centralised config profiles. These should not be edi Less common options for the pipeline, typically set in a config file. -| Parameter | Description | Type | Default | Required | Hidden | -| ---------------------------------- | ----------------------------------------------------------------------- | --------- | ------- | -------- | ------ | -| `help` | Display help text. | `boolean` | | | True | -| `version` | Display version and exit. | `boolean` | | | True | -| `publish_dir_mode` | Method used to save pipeline results to output directory. | `string` | copy | | True | -| `email_on_fail` | Email address for completion summary, only when pipeline fails. | `string` | | | True | -| `plaintext_email` | Send plain-text email instead of HTML. | `boolean` | | | True | -| `monochrome_logs` | Do not use coloured log outputs. | `boolean` | | | True | -| `hook_url` | Incoming hook URL for messaging service | `string` | | | True | -| `validate_params` | Boolean whether to validate parameters against the schema at runtime | `boolean` | True | | True | +| Parameter | Description | Type | Default | Required | Hidden | +| ------------------ | --------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `help` | Display help text. | `boolean` | | | True | +| `version` | Display version and exit. | `boolean` | | | True | +| `publish_dir_mode` | Method used to save pipeline results to output directory. | `string` | copy | | True | +| `email_on_fail` | Email address for completion summary, only when pipeline fails. | `string` | | | True | +| `plaintext_email` | Send plain-text email instead of HTML. | `boolean` | | | True | +| `monochrome_logs` | Do not use coloured log outputs. | `boolean` | | | True | +| `hook_url` | Incoming hook URL for messaging service | `string` | | | True | diff --git a/main.nf b/main.nf index b34069dd..7e46ba85 100755 --- a/main.nf +++ b/main.nf @@ -66,7 +66,6 @@ workflow { // PIPELINE_INITIALISATION ( params.version, - params.validate_params, params.monochrome_logs, args, params.outdir, diff --git a/nextflow.config b/nextflow.config index c002d677..b0065cc4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -90,10 +90,6 @@ params { config_profile_description = null custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - - // Schema validation default options - validate_params = true - } // Max resources @@ -292,6 +288,7 @@ validation { fullParameter = "help_full" showHiddenParameter = "show_hidden" } + monochromeLogs = params.monochrome_logs } // Load modules.config for DSL2 module specific options diff --git a/nextflow_schema.json b/nextflow_schema.json index 082ba205..ce8090db 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -428,13 +428,6 @@ "description": "Incoming hook URL for messaging service", "fa_icon": "fas fa-people-group", "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true } } } diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf index 11bdc8bc..c7634358 100644 --- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf @@ -29,7 +29,6 @@ workflow PIPELINE_INITIALISATION { take: version // boolean: Display version and exit - validate_params // boolean: Boolean whether to validate parameters against the schema at runtime monochrome_logs // boolean: Do not use coloured log outputs nextflow_cli_args // array: List of positional nextflow CLI args outdir // string: The output directory where the results will be saved @@ -56,8 +55,8 @@ workflow PIPELINE_INITIALISATION { // UTILS_NFSCHEMA_PLUGIN ( workflow, - validate_params, - null + true, // validate params + null // schema path: nextflow_schema ) From 9096eb8d0fef75c08bfdceff46b41dc79c73575b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:05:48 +1300 Subject: [PATCH 16/62] Updated params --- .editorconfig | 1 + docs/output.md | 1 + docs/parameters.md | 21 +++++---------------- nextflow_schema.json | 3 --- 4 files changed, 7 insertions(+), 19 deletions(-) diff --git a/.editorconfig b/.editorconfig index c320a60c..87e16fad 100644 --- a/.editorconfig +++ b/.editorconfig @@ -51,3 +51,4 @@ indent_style = unset # ignore perl [*.{pl,pm}] indent_size = unset +indent_style = unset diff --git a/docs/output.md b/docs/output.md index 97f67372..f5bfacff 100644 --- a/docs/output.md +++ b/docs/output.md @@ -11,6 +11,7 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: + - [FASTA and GFF3 validation](#fasta-and-gff3-validation) - [Assemblathon stats](#assemblathon-stats) - [Gfastats](#gfastats) diff --git a/docs/parameters.md b/docs/parameters.md index 6453ce9b..a6b2e912 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -4,11 +4,11 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and ## Input/output options -| Parameter | Description | Type | Default | Required | Hidden | -| --------- | ------------------------------------------------------------------------------------------------------------------------ | -------- | --------- | -------- | ------ | -| `input` | Input assembly sheet in CSV format | `string` | | True | | -| `outdir` | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure. | `string` | ./results | True | | -| `email` | Email address for completion summary. | `string` | | | | +| Parameter | Description | Type | Default | Required | Hidden | +| --------- | ------------------------------------------------------------------------------------------------------------------------ | -------- | ------- | -------- | ------ | +| `input` | Input assembly sheet in CSV format | `string` | | True | | +| `outdir` | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure. | `string` | | True | | +| `email` | Email address for completion summary. | `string` | | | | ## Validation options @@ -99,16 +99,6 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and | `merqury_skip` | Skip merqury analysis | `boolean` | True | | | | `merqury_kmer_length` | kmer length for merqury analysis | `integer` | 21 | | | -## Max job request options - -Set the top limit for requested resources for any single job. - -| Parameter | Description | Type | Default | Required | Hidden | -| ------------ | ---------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | -| `max_cpus` | Maximum number of CPUs that can be requested for any single job. | `integer` | 16 | | True | -| `max_memory` | Maximum amount of memory that can be requested for any single job. Example: '8.GB' | `string` | 512.GB | | True | -| `max_time` | Maximum amount of time that can be requested for any single job. Example: '1.day' | `string` | 7.day | | True | - ## Institutional config options Parameters used to describe centralised config profiles. These should not be edited. @@ -126,7 +116,6 @@ Less common options for the pipeline, typically set in a config file. | Parameter | Description | Type | Default | Required | Hidden | | ------------------ | --------------------------------------------------------------- | --------- | ------- | -------- | ------ | -| `help` | Display help text. | `boolean` | | | True | | `version` | Display version and exit. | `boolean` | | | True | | `publish_dir_mode` | Method used to save pipeline results to output directory. | `string` | copy | | True | | `email_on_fail` | Email address for completion summary, only when pipeline fails. | `string` | | | True | diff --git a/nextflow_schema.json b/nextflow_schema.json index ce8090db..eda14c7a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -436,9 +436,6 @@ { "$ref": "#/$defs/input_output_options" }, - { - "$ref": "#/$defs/institutional_config_options" - }, { "$ref": "#/$defs/validation_options" }, From eeae0f2b4b72964f9681570a083dc7b3a732552d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:10:06 +1300 Subject: [PATCH 17/62] Updated CI --- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 11 +++++------ .github/workflows/fix-linting.yml | 2 +- 3 files changed, 7 insertions(+), 8 deletions(-) diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index c5f090a7..81a9eaac 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -11,7 +11,7 @@ jobs: steps: # PRs to the nf-core repo main branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs - if: github.repository == 'plant-food-research-open/assemblyqc' + if: github.repository == 'Plant-Food-Research-Open/assemblyqc' run: | { [[ ${{ github.event.pull_request.head.repo.full_name }} == Plant-Food-Research-Open/assemblyqc ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index cbc81596..489cbfaf 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -54,16 +54,15 @@ jobs: run: | nextflow run \ ${GITHUB_WORKSPACE} \ - --outdir ./results \ - -profile docker \ - -params-file \ - ./tests/${{ matrix.TEST_PARAMS }}/params.json \ - ${{ matrix.OPTION_STUB }} + -profile test,docker \ + -params-file ./tests/${{ matrix.TEST_PARAMS }}/params.json \ + ${{ matrix.OPTION_STUB }} \ + --outdir ./results nf-test: name: Run nf-tests # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'plant-food-research-open/assemblyqc') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'Plant-Food-Research-Open/assemblyqc') }}" runs-on: ubuntu-latest strategy: matrix: diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 31ca9786..f1e67583 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -9,7 +9,7 @@ jobs: if: > contains(github.event.comment.html_url, '/pull/') && contains(github.event.comment.body, '@nf-core-bot fix linting') && - github.repository == 'plant-food-research-open/assemblyqc' + github.repository == 'Plant-Food-Research-Open/assemblyqc' runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later From 3946d57bc11830ca5dae84eec08901acd891c9fe Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:21:01 +1300 Subject: [PATCH 18/62] Fixed CI and linting issues --- .github/workflows/ci.yml | 2 +- .github/workflows/linting.yml | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 489cbfaf..583783db 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -67,7 +67,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.04.0" + - "24.04.2" steps: - name: Check out pipeline code uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 1bb5ff31..48ffb883 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -45,7 +45,7 @@ jobs: uses: pietrobolcato/action-read-yaml@1.0.0 id: read_yml with: - config: ${{ github.workspace }}/.nf-core.yaml + config: ${{ github.workspace }}/.nf-core.yml - name: Install dependencies run: | From 717fbf2eb26554ccf8e3f22762305bf332f5438d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:27:32 +1300 Subject: [PATCH 19/62] Linting fix --- .github/workflows/linting.yml | 2 +- .nf-core.yml | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 48ffb883..6bfe9373 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -42,7 +42,7 @@ jobs: architecture: "x64" - name: read .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.0.0 + uses: pietrobolcato/action-read-yaml@1.1.0 id: read_yml with: config: ${{ github.workspace }}/.nf-core.yml diff --git a/.nf-core.yml b/.nf-core.yml index cb312f8c..a021ce5d 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -43,5 +43,5 @@ template: - igenomes - multiqc - fastqc - version: 2.2.0+dev + version: 3.0.0 update: null From 559266e109b13e47accfa16e5c6756043dff8d6b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:32:06 +1300 Subject: [PATCH 20/62] Fixed nf-test --- tests/hicparam/hicparam.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/hicparam/hicparam.nf b/tests/hicparam/hicparam.nf index f3080271..b15416b4 100644 --- a/tests/hicparam/hicparam.nf +++ b/tests/hicparam/hicparam.nf @@ -3,7 +3,7 @@ import groovy.json.JsonSlurper def checkHiCParam(paramValue, schema) { def jsonSlurper = new JsonSlurper() def jsonContent = jsonSlurper.parse ( file ( schema, checkIfExists: true ) ) - def pattern = jsonContent.definitions.hic_options.properties.hic.pattern + def pattern = jsonContent['$defs'].hic_options.properties.hic.pattern def match = paramValue ==~ pattern return match From eeaaf3f8eae12694db61dbd78ae5e81d73a8e5e0 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 16:10:35 +1300 Subject: [PATCH 21/62] Fixed a bug where Gene score distribution graph did not appear correctly --- CHANGELOG.md | 2 + .../parsers/genometools_gt_stat_parser.py | 84 ++++++++++--------- tests/tiny/assemblysheet.csv | 2 + tests/tiny/params.json | 8 ++ 4 files changed, 55 insertions(+), 41 deletions(-) create mode 100644 tests/tiny/assemblysheet.csv create mode 100644 tests/tiny/params.json diff --git a/CHANGELOG.md b/CHANGELOG.md index b7ff96cb..1e1eeb34 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -12,6 +12,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Fixed` +1. Fixed a bug where Gene score distribution graph did not appear correctly [#125](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/125) + ### `Dependencies` 1. Nextflow!>=23.04.0 diff --git a/bin/report_modules/parsers/genometools_gt_stat_parser.py b/bin/report_modules/parsers/genometools_gt_stat_parser.py index 13f975a5..9881f576 100644 --- a/bin/report_modules/parsers/genometools_gt_stat_parser.py +++ b/bin/report_modules/parsers/genometools_gt_stat_parser.py @@ -22,7 +22,6 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): data = {"GENOMETOOLS_GT_STAT": []} for report_path in list_of_report_files: - NUM_GROUPS = -1 ( report_table_dict, @@ -35,7 +34,7 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): ) = extract_report_data(report_path, NUM_GROUPS) gene_length_distribution_graph = "" - if gene_length_distribution != []: + if len(gene_length_distribution) > 1: gene_length_distribution_graph = create_dist_graph( gene_length_distribution, "Length", @@ -44,7 +43,7 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): ) gene_score_distribution_graph = "" - if gene_score_distribution != []: + if len(gene_score_distribution) > 1: gene_score_distribution_graph = create_dist_graph( gene_score_distribution, "Score", @@ -53,7 +52,7 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): ) exon_length_distribution_graph = "" - if exon_length_distribution != []: + if len(exon_length_distribution) > 1: exon_length_distribution_graph = create_dist_graph( exon_length_distribution, "Length", @@ -62,7 +61,7 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): ) exon_number_distribution_graph = "" - if exon_number_distribution != []: + if len(exon_number_distribution) > 1: exon_number_distribution_graph = create_dist_graph( exon_number_distribution, "Number", @@ -71,7 +70,7 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): ) intron_length_distribution_graph = "" - if intron_length_distribution != []: + if len(intron_length_distribution) > 1: intron_length_distribution_graph = create_dist_graph( intron_length_distribution, "Length", @@ -80,7 +79,7 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): ) cds_length_distribution_graph = "" - if cds_length_distribution != []: + if len(cds_length_distribution) > 1: cds_length_distribution_graph = create_dist_graph( cds_length_distribution, "Length", @@ -196,7 +195,6 @@ def extract_report_data(report_path, num_groups): def create_frequency_groups(data, num_groups): - if num_groups == -1: sorted_data = sorted(data, key=lambda x: x[0]) return [ @@ -282,7 +280,6 @@ def test_create_frequency_groups_repeat(): def create_dist_graph(groups_dict, x_label, title, file_name): - x_list = [i["stop"] for i in groups_dict] y_list = [i["freq"] for i in groups_dict] sum_y = float(sum(y_list)) @@ -299,41 +296,46 @@ def create_dist_graph(groups_dict, x_label, title, file_name): plt.gca().spines["top"].set_visible(False) plt.gca().spines["right"].set_visible(False) - min_x, min_y = (min(x_list), min(y_list)) - x_anno_step = int(float(max(x_list)) * 0.1) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y + 10), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) + if len(y_list) >= 10: + max_x = max(x_list) + min_x, min_y = (min(x_list), min(y_list)) + x_anno_step = int(float(max(x_list)) * 0.1) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y + 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) - near_50 = min([y for y in y_list if y >= 50.0]) - min_x, min_y = (x_list[y_list.index(near_50)], near_50) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) + near_50 = min([y for y in y_list if y >= 50.0]) + min_x, min_y = (x_list[y_list.index(near_50)], near_50) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) - near_90 = min([y for y in y_list if y >= 90.0]) - min_x, min_y = (x_list[y_list.index(near_90)], near_90) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y - 10), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) + near_90 = min([y for y in y_list if y >= 90.0]) + min_x, min_y = (x_list[y_list.index(near_90)], near_90) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y - 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) - near_3_sigma = min([y for y in y_list if y >= 99.7]) - min_x, min_y = (x_list[y_list.index(near_3_sigma)], near_3_sigma) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y - 10), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) + near_3_sigma = min([y for y in y_list if y >= 99.7]) + min_x, min_y = (x_list[y_list.index(near_3_sigma)], near_3_sigma) + x_anno_step_updated = ( + x_anno_step if ((min_x + 2 * x_anno_step) < max_x) else (-2 * x_anno_step) + ) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step_updated, min_y - 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) plt.savefig(file_name, dpi=300) diff --git a/tests/tiny/assemblysheet.csv b/tests/tiny/assemblysheet.csv new file mode 100644 index 00000000..196f70e4 --- /dev/null +++ b/tests/tiny/assemblysheet.csv @@ -0,0 +1,2 @@ +tag,fasta,gff3 +sarscov2,https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/genome/genome.fasta,https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/genome/genome.gff3 diff --git a/tests/tiny/params.json b/tests/tiny/params.json new file mode 100644 index 00000000..58137a9b --- /dev/null +++ b/tests/tiny/params.json @@ -0,0 +1,8 @@ +{ + "config_profile_name": "Test profile", + "config_profile_description": "Tiny test dataset to check pipeline function", + "input": "tests/tiny/assemblysheet.csv", + "max_cpus": 2, + "max_memory": "6.GB", + "max_time": "6.h" +} From 6a77321695e01a006278327a8126d45dda20545b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 9 Oct 2024 22:55:37 +1300 Subject: [PATCH 22/62] Fixed ci and params --- .github/workflows/template_version_comment.yml | 2 +- tests/tiny/params.json | 5 +---- 2 files changed, 2 insertions(+), 5 deletions(-) diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml index 9dea41f0..d01fb43f 100644 --- a/.github/workflows/template_version_comment.yml +++ b/.github/workflows/template_version_comment.yml @@ -12,7 +12,7 @@ jobs: uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Read template version from .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.0.0 + uses: pietrobolcato/action-read-yaml@1.1.0 id: read_yml with: config: ${{ github.workspace }}/.nf-core.yml diff --git a/tests/tiny/params.json b/tests/tiny/params.json index 58137a9b..71262048 100644 --- a/tests/tiny/params.json +++ b/tests/tiny/params.json @@ -1,8 +1,5 @@ { "config_profile_name": "Test profile", "config_profile_description": "Tiny test dataset to check pipeline function", - "input": "tests/tiny/assemblysheet.csv", - "max_cpus": 2, - "max_memory": "6.GB", - "max_time": "6.h" + "input": "tests/tiny/assemblysheet.csv" } From 966c6b25a6e496c208fadb4aee047b085de94aed Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 11:01:34 +1300 Subject: [PATCH 23/62] Template update for nf-core/tools version 3.0.1 --- .editorconfig | 4 - .github/CONTRIBUTING.md | 2 +- .github/workflows/linting.yml | 4 +- .nf-core.yml | 23 +- .prettierignore | 1 - CHANGELOG.md | 2 +- README.md | 2 +- assets/email_template.html | 150 +++------- modules.json | 4 +- nextflow.config | 14 +- .../utils_nfcore_assemblyqc_pipeline/main.nf | 12 +- .../nf-core/utils_nextflow_pipeline/main.nf | 46 ++- .../nf-core/utils_nfcore_pipeline/main.nf | 279 ++++++++++-------- 13 files changed, 245 insertions(+), 298 deletions(-) diff --git a/.editorconfig b/.editorconfig index e1058815..72dda289 100644 --- a/.editorconfig +++ b/.editorconfig @@ -11,7 +11,6 @@ indent_style = space [*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 - # These files are edited and tested upstream in nf-core/modules [/modules/nf-core/**] charset = unset @@ -26,12 +25,9 @@ insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset - - [/assets/email*] indent_size = unset - # ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 1391e6f0..d8dd3c9d 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -81,7 +81,7 @@ Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json ### Default processes resource requirements -Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b882838a..a502573c 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -42,10 +42,10 @@ jobs: architecture: "x64" - name: read .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.0.0 + uses: pietrobolcato/action-read-yaml@1.1.0 id: read_yml with: - config: ${{ github.workspace }}/.nf-core.yaml + config: ${{ github.workspace }}/.nf-core.yml - name: Install dependencies run: | diff --git a/.nf-core.yml b/.nf-core.yml index 56ab1ec3..e4559f27 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,9 +1,6 @@ bump_version: null lint: files_exist: - - conf/igenomes.config - - conf/igenomes_ignored.config - - assets/multiqc_config.yml - conf/igenomes.config - conf/igenomes_ignored.config - assets/multiqc_config.yml @@ -15,15 +12,12 @@ lint: - .github/workflows/awstest.yml - .github/workflows/awsfulltest.yml files_unchanged: + - LICENSE - .github/CONTRIBUTING.md - - assets/sendmail_template.txt - - .github/CONTRIBUTING.md - - assets/sendmail_template.txt - - CODE_OF_CONDUCT.md - - assets/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_dark.png - - .github/ISSUE_TEMPLATE/bug_report.yml + - .github/PULL_REQUEST_TEMPLATE.md + - .github/workflows/branch.yml + - .github/workflows/linting.yml + - docs/README.md multiqc_config: false nextflow_config: - manifest.name @@ -32,14 +26,15 @@ lint: - validation.help.afterText - validation.summary.beforeText - validation.summary.afterText -nf_core_version: 3.0.0 + template_strings: false +nf_core_version: 3.0.1 org_path: null repository_type: pipeline template: author: Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy description: A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. - force: true + force: false is_nfcore: false name: assemblyqc org: plant-food-research-open @@ -48,5 +43,5 @@ template: - igenomes - multiqc - fastqc - version: 1.0.0dev + version: 3.0.0 update: null diff --git a/.prettierignore b/.prettierignore index 610e5069..437d763d 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,4 +1,3 @@ - email_template.html adaptivecard.json slackreport.json diff --git a/CHANGELOG.md b/CHANGELOG.md index 7f0eabfe..c7caa0f1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0.0+dev - [date] +## v3.0.0 - [date] Initial release of plant-food-research-open/assemblyqc, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index 73512f1d..392fad19 100644 --- a/README.md +++ b/README.md @@ -80,7 +80,7 @@ If you would like to contribute to this pipeline, please see the [contributing g An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. -This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). +This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/main/LICENSE). > **The nf-core framework for community-curated bioinformatics pipelines.** > diff --git a/assets/email_template.html b/assets/email_template.html index 49545d50..5a56215f 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,119 +1,53 @@ - - - - + + + + - - plant-food-research-open/assemblyqc Pipeline Report - - -
    - + + plant-food-research-open/assemblyqc Pipeline Report + + +
    -

    plant-food-research-open/assemblyqc ${version}

    -

    Run Name: $runName

    + - <% if (!success){ out << """ -
    -

    - plant-food-research-open/assemblyqc execution completed unsuccessfully! -

    +

    plant-food-research-open/assemblyqc ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    plant-food-research-open/assemblyqc execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    -
    ${errorReport}
    -
    - """ } else { out << """ -
    +
    ${errorReport}
    +
    + """ +} else { + out << """ +
    plant-food-research-open/assemblyqc execution completed successfully! -
    - """ } %> +
    + """ +} +%> -

    The workflow was completed at $dateComplete (duration: $duration)

    -

    The command used to launch the workflow was as follows:

    -
    -$commandLine
    +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    -

    Pipeline Configuration:

    - - - <% out << summary.collect{ k,v -> " - - - - - " }.join("\n") %> - -
    - $k - -
    $v
    -
    +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    -

    plant-food-research-open/assemblyqc

    -

    - https://github.com/plant-food-research-open/assemblyqc -

    -
    - +

    plant-food-research-open/assemblyqc

    +

    https://github.com/plant-food-research-open/assemblyqc

    + +
    + + diff --git a/modules.json b/modules.json index 5f5fbb68..b221651c 100644 --- a/modules.json +++ b/modules.json @@ -10,12 +10,12 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "d20fb2a9cc3e2835e9d067d1046a63252eb17352", + "git_sha": "9d05360da397692321d377b6102d2fb22507c6ef", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "2fdce49d30c0254f76bc0f13c55c17455c1251ab", + "git_sha": "772684d9d66f37b650c8ba5146ac1ee3ecba2acb", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { diff --git a/nextflow.config b/nextflow.config index 7cb553f5..4d55e9cc 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,6 +13,10 @@ params { // Input options input = null + + + + // Boilerplate options outdir = null publish_dir_mode = 'copy' @@ -26,6 +30,7 @@ params { show_hidden = false version = false pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' + // Config options config_profile_name = null config_profile_description = null @@ -34,9 +39,9 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null + // Schema validation default options validate_params = true - } // Load base.config by default for all pipelines @@ -151,6 +156,7 @@ includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${pa // Load plant-food-research-open/assemblyqc custom profiles from different institutions. // TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs // includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/assemblyqc.config" : "/dev/null" + // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled // Set to your registry if you have a mirror of containers @@ -160,6 +166,8 @@ podman.registry = 'quay.io' singularity.registry = 'quay.io' charliecloud.registry = 'quay.io' + + // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -209,7 +217,7 @@ manifest { description = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=24.04.2' - version = '1.0.0dev' + version = '3.0.0' doi = '' } @@ -225,9 +233,9 @@ validation { command = "nextflow run $manifest.name -profile --input samplesheet.csv --outdir " fullParameter = "help_full" showHiddenParameter = "show_hidden" + } } // Load modules.config for DSL2 module specific options includeConfig 'conf/modules.config' - diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf index fdc1719f..e3af7ecb 100644 --- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf @@ -18,9 +18,9 @@ include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW TO INITIALISE PIPELINE -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow PIPELINE_INITIALISATION { @@ -95,9 +95,9 @@ workflow PIPELINE_INITIALISATION { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW FOR PIPELINE COMPLETION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow PIPELINE_COMPLETION { @@ -143,9 +143,9 @@ workflow PIPELINE_COMPLETION { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 28e32b20..2b0dc67a 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -3,13 +3,12 @@ // /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NEXTFLOW_PIPELINE { - take: print_version // boolean: print version dump_parameters // boolean: dump parameters @@ -22,7 +21,7 @@ workflow UTILS_NEXTFLOW_PIPELINE { // Print workflow version and exit on --version // if (print_version) { - log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + log.info("${workflow.manifest.name} ${getWorkflowVersion()}") System.exit(0) } @@ -45,9 +44,9 @@ workflow UTILS_NEXTFLOW_PIPELINE { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -72,11 +71,11 @@ def getWorkflowVersion() { // Dump pipeline parameters to a JSON file // def dumpParametersToJSON(outdir) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = groovy.json.JsonOutput.toJson(params) - temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) + def timestamp = new java.util.Date().format('yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = groovy.json.JsonOutput.toJson(params) + temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") temp_pf.delete() @@ -91,9 +90,14 @@ def checkCondaChannels() { try { def config = parser.load("conda config --show channels".execute().text) channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return + } + catch (NullPointerException e) { + log.warn("Could not verify conda channel configuration.") + return null + } + catch (IOException e) { + log.warn("Could not verify conda channel configuration.") + return null } // Check that all channels are present @@ -106,19 +110,13 @@ def checkCondaChannels() { required_channels_in_order.eachWithIndex { channel, index -> if (index < required_channels_in_order.size() - 1) { - channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index+1])) + channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index + 1])) } } if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + log.warn( + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " There is a problem with your Conda configuration!\n\n" + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + " Please refer to https://bioconda.github.io/\n" + " The observed channel order is \n" + " ${channels}\n" + " but the following channel order is required:\n" + " ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + ) } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index cbd8495b..b78273ca 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -3,13 +3,12 @@ // /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NFCORE_PIPELINE { - take: nextflow_cli_args @@ -22,9 +21,9 @@ workflow UTILS_NFCORE_PIPELINE { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -33,12 +32,9 @@ workflow UTILS_NFCORE_PIPELINE { def checkConfigProvided() { def valid_config = true as Boolean if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " + log.warn( + "[${workflow.manifest.name}] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + "Please refer to the quick start section and usage docs for the pipeline.\n " + ) valid_config = false } return valid_config @@ -49,12 +45,14 @@ def checkConfigProvided() { // def checkProfileProvided(nextflow_cli_args) { if (workflow.profile.endsWith(',')) { - error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + - "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + error( + "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + ) } if (nextflow_cli_args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + - "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + log.warn( + "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + ) } } @@ -70,13 +68,7 @@ def workflowCitation() { manifest_doi.each { doi_ref -> temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" } - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - temp_doi_ref + "\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } // @@ -102,7 +94,7 @@ def getWorkflowVersion() { // def processVersionsFromYAML(yaml_file) { def yaml = new org.yaml.snakeyaml.Yaml() - def versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + def versions = yaml.load(yaml_file).collectEntries { k, v -> [k.tokenize(':')[-1], v] } return yaml.dumpAsMap(versions).trim() } @@ -112,8 +104,8 @@ def processVersionsFromYAML(yaml_file) { def workflowVersionToYAML() { return """ Workflow: - $workflow.manifest.name: ${getWorkflowVersion()} - Nextflow: $workflow.nextflow.version + ${workflow.manifest.name}: ${getWorkflowVersion()} + Nextflow: ${workflow.nextflow.version} """.stripIndent().trim() } @@ -121,11 +113,7 @@ def workflowVersionToYAML() { // Get channel of software versions used in pipeline in YAML format // def softwareVersionsToYAML(ch_versions) { - return ch_versions - .unique() - .map { version -> processVersionsFromYAML(version) } - .unique() - .mix(Channel.of(workflowVersionToYAML())) + return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML())) } // @@ -133,25 +121,31 @@ def softwareVersionsToYAML(ch_versions) { // def paramsSummaryMultiqc(summary_params) { def summary_section = '' - summary_params.keySet().each { group -> - def group_params = summary_params.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - group_params.keySet().sort().each { param -> - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + summary_params + .keySet() + .each { group -> + def group_params = summary_params.get(group) + // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    ${group}

    \n" + summary_section += "
    \n" + group_params + .keySet() + .sort() + .each { param -> + summary_section += "
    ${param}
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" } - summary_section += "
    \n" } - } - def yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" as String - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" + def yaml_file_text = "id: '${workflow.manifest.name.replace('/', '-')}-summary'\n" as String + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" return yaml_file_text } @@ -199,54 +193,54 @@ def logColours(monochrome_logs=true) { colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" return colorcodes } @@ -261,14 +255,15 @@ def attachMultiqcReport(multiqc_report) { mqc_report = multiqc_report.getVal() if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one") } mqc_report = mqc_report[0] } } - } catch (all) { + } + catch (Exception all) { if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email") } } return mqc_report @@ -280,26 +275,35 @@ def attachMultiqcReport(multiqc_report) { def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + def subject = "[${workflow.manifest.name}] Successful: ${workflow.runName}" if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + subject = "[${workflow.manifest.name}] FAILED: ${workflow.runName}" } def summary = [:] - summary_params.keySet().sort().each { group -> - summary << summary_params[group] - } + summary_params + .keySet() + .sort() + .each { group -> + summary << summary_params[group] + } def misc_fields = [:] misc_fields['Date Started'] = workflow.start misc_fields['Date Completed'] = workflow.complete misc_fields['Pipeline script file path'] = workflow.scriptFile misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build + if (workflow.repository) { + misc_fields['Pipeline repository Git URL'] = workflow.repository + } + if (workflow.commitId) { + misc_fields['Pipeline repository Git Commit'] = workflow.commitId + } + if (workflow.revision) { + misc_fields['Pipeline Git branch/tag'] = workflow.revision + } + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp def email_fields = [:] @@ -337,7 +341,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Render the sendmail template def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def smail_fields = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() @@ -346,30 +350,32 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi def colors = logColours(monochrome_logs) as Map if (email_address) { try { - if (plaintext_email) { throw new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } + if (plaintext_email) { +new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { + ['sendmail', '-t'].execute() << sendmail_html + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-") + } + catch (Exception all) { // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address] mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (mail)-") } } // Write summary e-mail HTML to a file def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html") output_hf.delete() // Write summary e-mail TXT to a file def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } - nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt") output_tf.delete() } @@ -380,12 +386,14 @@ def completionSummary(monochrome_logs=true) { def colors = logColours(monochrome_logs) as Map if (workflow.success) { if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Pipeline completed successfully${colors.reset}-") + } + else { + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-") } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + else { + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.red} Pipeline completed with errors${colors.reset}-") } } @@ -394,21 +402,30 @@ def completionSummary(monochrome_logs=true) { // def imNotification(summary_params, hook_url) { def summary = [:] - summary_params.keySet().sort().each { group -> - summary << summary_params[group] - } + summary_params + .keySet() + .sort() + .each { group -> + summary << summary_params[group] + } def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) { + misc_fields['repository'] = workflow.repository + } + if (workflow.commitId) { + misc_fields['commitid'] = workflow.commitId + } + if (workflow.revision) { + misc_fields['revision'] = workflow.revision + } + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp def msg_fields = [:] msg_fields['version'] = getWorkflowVersion() @@ -433,13 +450,13 @@ def imNotification(summary_params, hook_url) { def json_message = json_template.toString() // POST - def post = new URL(hook_url).openConnection(); + def post = new URL(hook_url).openConnection() post.setRequestMethod("POST") post.setDoOutput(true) post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); + post.getOutputStream().write(json_message.getBytes("UTF-8")) + def postRC = post.getResponseCode() + if (!postRC.equals(200)) { + log.warn(post.getErrorStream().getText()) } } From d2f537c74355212220faec4d4bf4771b5324a86a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 12:44:59 +1300 Subject: [PATCH 24/62] Updated version checks --- .github/version_checks.sh | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/.github/version_checks.sh b/.github/version_checks.sh index 23cf619c..e7b1a90a 100755 --- a/.github/version_checks.sh +++ b/.github/version_checks.sh @@ -12,3 +12,8 @@ fi head -10 CHANGELOG.md | grep "## v$config_version - " >/dev/null \ || (echo 'Failed to match CHANGELOG version'; exit 1) + +# Check .nf-core.yml version + +tail -5 .nf-core.yml | grep "version: $config_version" >/dev/null \ + || (echo 'Failed to match .nf-core.yml version'; exit 1) From 530734b73c289022b7ab3eae51c3c4d44541120c Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 12:51:26 +1300 Subject: [PATCH 25/62] Deleted template version comment action --- .../workflows/template_version_comment.yml | 43 ------------------- 1 file changed, 43 deletions(-) delete mode 100644 .github/workflows/template_version_comment.yml diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml deleted file mode 100644 index d01fb43f..00000000 --- a/.github/workflows/template_version_comment.yml +++ /dev/null @@ -1,43 +0,0 @@ -name: nf-core template version comment -# This workflow is triggered on PRs to check if the pipeline template version matches the latest nf-core version. -# It posts a comment to the PR, even if it comes from a fork. - -on: pull_request_target - -jobs: - template_version: - runs-on: ubuntu-latest - steps: - - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - - name: Read template version from .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.1.0 - id: read_yml - with: - config: ${{ github.workspace }}/.nf-core.yml - - - name: Install nf-core - run: | - python -m pip install --upgrade pip - pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} - - - name: Check nf-core outdated - id: nf_core_outdated - run: pip list --outdated | grep nf-core - - - name: Post nf-core template version comment - uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 - if: | - ${{ steps.nf_core_outdated.outputs.stdout }} =~ 'nf-core' - with: - repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }} - allow-repeats: false - message: | - ## :warning: Newer version of the nf-core template is available. - - Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}. - Please update your pipeline to the latest version. - - For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync). - # From b36e86862c3f62c30983dc55d4fa2e702acd9838 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 12:52:20 +1300 Subject: [PATCH 26/62] Removed fix-linting action --- .github/workflows/fix-linting.yml | 89 ------------------------------- 1 file changed, 89 deletions(-) delete mode 100644 .github/workflows/fix-linting.yml diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml deleted file mode 100644 index f1e67583..00000000 --- a/.github/workflows/fix-linting.yml +++ /dev/null @@ -1,89 +0,0 @@ -name: Fix linting from a comment -on: - issue_comment: - types: [created] - -jobs: - fix-linting: - # Only run if comment is on a PR with the main repo, and if it contains the magic keywords - if: > - contains(github.event.comment.html_url, '/pull/') && - contains(github.event.comment.body, '@nf-core-bot fix linting') && - github.repository == 'Plant-Food-Research-Open/assemblyqc' - runs-on: ubuntu-latest - steps: - # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - with: - token: ${{ secrets.nf_core_bot_auth_token }} - - # indication that the linting is being fixed - - name: React on comment - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - comment-id: ${{ github.event.comment.id }} - reactions: eyes - - # Action runs on the issue comment, so we don't get the PR by default - # Use the gh cli to check out the PR - - name: Checkout Pull Request - run: gh pr checkout ${{ github.event.issue.number }} - env: - GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - # Install and run pre-commit - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 - with: - python-version: "3.12" - - - name: Install pre-commit - run: pip install pre-commit - - - name: Run pre-commit - id: pre-commit - run: pre-commit run --all-files - continue-on-error: true - - # indication that the linting has finished - - name: react if linting finished succesfully - if: steps.pre-commit.outcome == 'success' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - comment-id: ${{ github.event.comment.id }} - reactions: "+1" - - - name: Commit & push changes - id: commit-and-push - if: steps.pre-commit.outcome == 'failure' - run: | - git config user.email "core@nf-co.re" - git config user.name "nf-core-bot" - git config push.default upstream - git add . - git status - git commit -m "[automated] Fix code linting" - git push - - - name: react if linting errors were fixed - id: react-if-fixed - if: steps.commit-and-push.outcome == 'success' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - comment-id: ${{ github.event.comment.id }} - reactions: hooray - - - name: react if linting errors were not fixed - if: steps.commit-and-push.outcome == 'failure' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - comment-id: ${{ github.event.comment.id }} - reactions: confused - - - name: react if linting errors were not fixed - if: steps.commit-and-push.outcome == 'failure' - uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 - with: - issue-number: ${{ github.event.issue.number }} - body: | - @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. - See [CI log](https://github.com/plant-food-research-open/assemblyqc/actions/runs/${{ github.run_id }}) for more details. From d7f67154e0be171656ee8f84dd2746665b44e589 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 12:55:07 +1300 Subject: [PATCH 27/62] Updated clean-up issue --- .github/workflows/clean-up.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 0b6b1f27..53e721c7 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -12,9 +12,9 @@ jobs: steps: - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: - stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." - stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." - close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." + stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback. Remove stale label or add a comment otherwise this issue will be closed in 20 days." + stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback. Remove stale label or add a comment if it is still useful." + close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback and then staled for 20 days with no activity." days-before-stale: 30 days-before-close: 20 days-before-pr-close: -1 From 5e4bdf5de5ea47f6ee209a80fa3774849235a317 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 13:22:19 +1300 Subject: [PATCH 28/62] Updated samtools faidx to 1.21 --- CHANGELOG.md | 1 + modules.json | 90 +++---- modules/gallvp/busco/busco/main.nf | 2 +- modules/gallvp/busco/busco/meta.yml | 172 ++++++++----- .../busco/busco/tests/main.nf.test.snap | 38 +-- modules/gallvp/busco/generateplot/meta.yml | 27 +- modules/gallvp/bwa/index/meta.yml | 41 +-- modules/gallvp/bwa/mem/meta.yml | 111 +++++---- modules/gallvp/cat/cat/meta.yml | 39 +-- modules/gallvp/custom/relabelfasta/meta.yml | 57 ++--- modules/gallvp/custom/restoregffids/meta.yml | 56 +++-- .../gallvp/custom/shortenfastaids/meta.yml | 60 +++-- modules/gallvp/gffread/meta.yml | 84 ++++--- modules/gallvp/gt/gff3/meta.yml | 59 +++-- modules/gallvp/gt/gff3validator/meta.yml | 56 +++-- modules/gallvp/gt/stat/meta.yml | 47 ++-- modules/gallvp/ltrfinder/meta.yml | 60 +++-- modules/gallvp/ltrfinder/tests/main.nf.test | 4 +- modules/gallvp/ltrharvest/meta.yml | 59 +++-- modules/gallvp/ltrretriever/lai/meta.yml | 88 ++++--- .../gallvp/ltrretriever/ltrretriever/meta.yml | 142 +++++++---- .../ltrretriever/tests/main.nf.test | 16 +- modules/gallvp/plotsr/meta.yml | 117 ++++----- modules/gallvp/samblaster/meta.yml | 45 ++-- modules/gallvp/samtools/faidx/environment.yml | 4 +- modules/gallvp/samtools/faidx/main.nf | 4 +- modules/gallvp/samtools/faidx/meta.yml | 85 ++++--- .../samtools/faidx/tests/main.nf.test.snap | 50 ++-- modules/gallvp/seqkit/seq/meta.yml | 47 ++-- modules/gallvp/syri/meta.yml | 90 ++++--- .../custom/sratoolsncbisettings/meta.yml | 20 +- modules/nf-core/fastavalidator/meta.yml | 56 +++-- modules/nf-core/fastp/meta.yml | 138 ++++++---- modules/nf-core/fastqc/meta.yml | 57 +++-- modules/nf-core/fcs/fcsadaptor/meta.yml | 91 ++++--- modules/nf-core/gunzip/meta.yml | 37 +-- modules/nf-core/merqury/hapmers/meta.yml | 115 +++++---- modules/nf-core/merqury/merqury/meta.yml | 235 ++++++++++++------ modules/nf-core/meryl/count/meta.yml | 53 ++-- modules/nf-core/meryl/unionsum/meta.yml | 49 ++-- modules/nf-core/minimap2/align/meta.yml | 115 +++++---- modules/nf-core/seqkit/rmdup/meta.yml | 59 +++-- modules/nf-core/seqkit/seq/meta.yml | 47 ++-- modules/nf-core/seqkit/sort/meta.yml | 44 ++-- modules/nf-core/sratools/fasterqdump/meta.yml | 71 +++--- modules/nf-core/sratools/prefetch/meta.yml | 72 +++--- modules/nf-core/tidk/explore/meta.yml | 63 +++-- modules/nf-core/tidk/plot/meta.yml | 47 ++-- modules/nf-core/tidk/search/meta.yml | 65 ++--- modules/nf-core/umitools/extract/meta.yml | 64 +++-- modules/nf-core/untar/meta.yml | 43 ++-- .../tests/main.nf.test.snap | 42 ++-- .../nf-core/utils_nextflow_pipeline/main.nf | 8 +- .../nf-core/utils_nfcore_pipeline/main.nf | 8 +- 54 files changed, 1940 insertions(+), 1410 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 386e75e1..99704fe9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 1. Added Gfastats [#126](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/126) 2. Updated nf-core/template to 3.0.1 [#149](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/149) +3. Updated `samtools faidx` to 1.21 ### `Fixed` diff --git a/modules.json b/modules.json index 2aaa9306..e5e4e979 100644 --- a/modules.json +++ b/modules.json @@ -7,107 +7,107 @@ "gallvp": { "busco/busco": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "92d59e5f578a2929b75f7588985b9bf451f4c370", "installed_by": ["fasta_gxf_busco_plot"] }, "busco/generateplot": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_gxf_busco_plot"] }, "bwa/index": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fastq_bwa_mem_samblaster"] }, "bwa/mem": { "branch": "main", - "git_sha": "a203a6035aed3f9b345ee380f5d497ca98504d98", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fastq_bwa_mem_samblaster"] }, "cat/cat": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_ltrretriever_lai"] }, "custom/relabelfasta": { "branch": "main", - "git_sha": "a203a6035aed3f9b345ee380f5d497ca98504d98", + "git_sha": "a8939d36280e7d9037c7cf164eeede19e46546a4", "installed_by": ["modules"] }, "custom/restoregffids": { "branch": "main", - "git_sha": "a203a6035aed3f9b345ee380f5d497ca98504d98", + "git_sha": "a8939d36280e7d9037c7cf164eeede19e46546a4", "installed_by": ["fasta_ltrretriever_lai"] }, "custom/shortenfastaids": { "branch": "main", - "git_sha": "a203a6035aed3f9b345ee380f5d497ca98504d98", + "git_sha": "a8939d36280e7d9037c7cf164eeede19e46546a4", "installed_by": ["fasta_ltrretriever_lai"] }, "gffread": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_gxf_busco_plot"] }, "gt/gff3": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["gff3_gt_gff3_gff3validator_stat"] }, "gt/gff3validator": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["gff3_gt_gff3_gff3validator_stat"] }, "gt/stat": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["gff3_gt_gff3_gff3validator_stat"] }, "ltrfinder": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_ltrretriever_lai"] }, "ltrharvest": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_ltrretriever_lai"] }, "ltrretriever/lai": { "branch": "main", - "git_sha": "cc6ea1f3e96e8264d2cc99afed31bcf3b0bb03ca", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_ltrretriever_lai"] }, "ltrretriever/ltrretriever": { "branch": "main", - 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"git_sha": "58c5f9e695b9e03d43e4c59d9339af7c93f0acbe", + "git_sha": "92d59e5f578a2929b75f7588985b9bf451f4c370", "installed_by": ["subworkflows"] } } @@ -142,27 +142,27 @@ "nf-core": { "custom/sratoolsncbisettings": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fastq_download_prefetch_fasterqdump_sratools"] }, "fastavalidator": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "fastp": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fastq_fastqc_umitools_fastp"] }, "fastqc": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fastq_fastqc_umitools_fastp"] }, "fcs/fcsadaptor": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "gfastats": { @@ -172,82 +172,82 @@ }, "gunzip": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "merqury/hapmers": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "merqury/merqury": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "meryl/count": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "meryl/unionsum": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "minimap2/align": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "seqkit/rmdup": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "seqkit/seq": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fasta_explore_search_plot_tidk", "fasta_ltrretriever_lai"] }, "seqkit/sort": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fasta_explore_search_plot_tidk"] }, "sratools/fasterqdump": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"] }, "sratools/prefetch": { "branch": "master", - "git_sha": "368e6c90b91adbd171e7c0a1c85a700b86a915af", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fastq_download_prefetch_fasterqdump_sratools", "modules"] }, "tidk/explore": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fasta_explore_search_plot_tidk"] }, "tidk/plot": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fasta_explore_search_plot_tidk"] }, "tidk/search": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fasta_explore_search_plot_tidk"] }, "umitools/extract": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["fastq_fastqc_umitools_fastp"] }, "untar": { "branch": "master", - "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] } } @@ -271,12 +271,12 @@ }, "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "9d05360da397692321d377b6102d2fb22507c6ef", + "git_sha": "f533459a222ac53eb4c6bb7a5f574e4069197cdb", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "772684d9d66f37b650c8ba5146ac1ee3ecba2acb", + "git_sha": "f533459a222ac53eb4c6bb7a5f574e4069197cdb", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { diff --git a/modules/gallvp/busco/busco/main.nf b/modules/gallvp/busco/busco/main.nf index f7c1a662..98cf5b04 100644 --- a/modules/gallvp/busco/busco/main.nf +++ b/modules/gallvp/busco/busco/main.nf @@ -11,7 +11,7 @@ process BUSCO_BUSCO { tuple val(meta), path(fasta, stageAs:'tmp_input/*') val mode // Required: One of genome, proteins, or transcriptome val lineage // Required: lineage to check against, "auto" enables --auto-lineage instead - path busco_lineages_path // Recommended: path to busco lineages - downloads if not set + path busco_lineages_path // Recommended: p_ath to busco lineages - downloads if not set path config_file // Optional: busco configuration file output: diff --git a/modules/gallvp/busco/busco/meta.yml b/modules/gallvp/busco/busco/meta.yml index 29745d2c..7cb6d69c 100644 --- a/modules/gallvp/busco/busco/meta.yml +++ b/modules/gallvp/busco/busco/meta.yml @@ -7,81 +7,135 @@ keywords: - proteome tools: - busco: - description: BUSCO provides measures for quantitative assessment of genome assembly, gene set, and transcriptome completeness based on evolutionarily informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB. + description: BUSCO provides measures for quantitative assessment of genome assembly, + gene set, and transcriptome completeness based on evolutionarily informed expectations + of gene content from near-universal single-copy orthologs selected from OrthoDB. homepage: https://busco.ezlab.org/ documentation: https://busco.ezlab.org/busco_userguide.html tool_dev_url: https://gitlab.com/ezlab/busco doi: "10.1007/978-1-4939-9173-0_14" licence: ["MIT"] + identifier: biotools:busco input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - fasta: - type: file - description: Nucleic or amino acid sequence file in FASTA format. - pattern: "*.{fasta,fna,fa,fasta.gz,fna.gz,fa.gz}" - - mode: - type: string - description: The mode to run Busco in. One of genome, proteins, or transcriptome - pattern: "{genome,proteins,transcriptome}" - - lineage: - type: string - description: The BUSCO lineage to use, or "auto" to automatically select lineage - - busco_lineages_path: - type: directory - description: Path to local BUSCO lineages directory. - - config_file: - type: file - description: Path to BUSCO config file. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Nucleic or amino acid sequence file in FASTA format. + pattern: "*.{fasta,fna,fa,fasta.gz,fna.gz,fa.gz}" + - - mode: + type: string + description: The mode to run Busco in. One of genome, proteins, or transcriptome + pattern: "{genome,proteins,transcriptome}" + - - lineage: + type: string + description: The BUSCO lineage to use, or "auto" to automatically select lineage + - - busco_lineages_path: + type: directory + description: Path to local BUSCO lineages directory. + - - config_file: + type: file + description: Path to BUSCO config file. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - batch_summary: - type: file - description: Summary of all sequence files analyzed - pattern: "*-busco.batch_summary.txt" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco.batch_summary.txt": + type: file + description: Summary of all sequence files analyzed + pattern: "*-busco.batch_summary.txt" - short_summaries_txt: - type: file - description: Short Busco summary in plain text format - pattern: "short_summary.*.txt" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - short_summary.*.txt: + type: file + description: Short Busco summary in plain text format + pattern: "short_summary.*.txt" - short_summaries_json: - type: file - description: Short Busco summary in JSON format - pattern: "short_summary.*.json" - - busco_dir: - type: directory - description: BUSCO lineage specific output - pattern: "*-busco" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - short_summary.*.json: + type: file + description: Short Busco summary in JSON format + pattern: "short_summary.*.json" - full_table: - type: file - description: Full BUSCO results table - pattern: "full_table.tsv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco/*/run_*/full_table.tsv": + type: file + description: Full BUSCO results table + pattern: "full_table.tsv" - missing_busco_list: - type: file - description: List of missing BUSCOs - pattern: "missing_busco_list.tsv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco/*/run_*/missing_busco_list.tsv": + type: file + description: List of missing BUSCOs + pattern: "missing_busco_list.tsv" - single_copy_proteins: - type: file - description: Fasta file of single copy proteins (transcriptome mode) - pattern: "single_copy_proteins.faa" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco/*/run_*/single_copy_proteins.faa": + type: file + description: Fasta file of single copy proteins (transcriptome mode) + pattern: "single_copy_proteins.faa" - seq_dir: - type: directory - description: BUSCO sequence directory - pattern: "busco_sequences" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco/*/run_*/busco_sequences": + type: directory + description: BUSCO sequence directory + pattern: "busco_sequences" - translated_dir: - type: directory - description: Six frame translations of each transcript made by the transcriptome mode - pattern: "translated_dir" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco/*/translated_proteins": + type: directory + description: Six frame translations of each transcript made by the transcriptome + mode + pattern: "translated_dir" + - busco_dir: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*-busco": + type: directory + description: BUSCO lineage specific output + pattern: "*-busco" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@priyanka-surana" - "@charles-plessy" diff --git a/modules/gallvp/busco/busco/tests/main.nf.test.snap b/modules/gallvp/busco/busco/tests/main.nf.test.snap index 1140d5de..825ddb98 100644 --- a/modules/gallvp/busco/busco/tests/main.nf.test.snap +++ b/modules/gallvp/busco/busco/tests/main.nf.test.snap @@ -11,19 +11,19 @@ ] ], "1": [ - + ], "2": [ - + ], "3": [ - + ], "4": [ - + ], "5": [ - + ], "6": [ [ @@ -31,12 +31,12 @@ "id": "test" }, [ - + ] ] ], "7": [ - + ], "8": [ [ @@ -47,7 +47,7 @@ [ [ [ - + ] ] ] @@ -74,7 +74,7 @@ [ [ [ - + ] ] ] @@ -82,10 +82,10 @@ ] ], "full_table": [ - + ], "missing_busco_list": [ - + ], "seq_dir": [ [ @@ -93,21 +93,21 @@ "id": "test" }, [ - + ] ] ], "short_summaries_json": [ - + ], "short_summaries_txt": [ - + ], "single_copy_proteins": [ - + ], "translated_dir": [ - + ], "versions": [ "versions.yml:md5,3fc94714b95c2dc15399a4229d9dd1d9" @@ -159,9 +159,9 @@ ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.04.4" + "nextflow": "23.10.1" }, - "timestamp": "2024-08-22T11:24:24.828742" + "timestamp": "2024-05-03T13:23:50.255602" }, "test_busco_eukaryote_metaeuk": { "content": [ @@ -227,4 +227,4 @@ }, "timestamp": "2024-05-03T13:27:12.724862" } -} \ No newline at end of file +} diff --git a/modules/gallvp/busco/generateplot/meta.yml b/modules/gallvp/busco/generateplot/meta.yml index 796f32b4..72ad2c92 100644 --- a/modules/gallvp/busco/generateplot/meta.yml +++ b/modules/gallvp/busco/generateplot/meta.yml @@ -9,26 +9,31 @@ keywords: - quality control tools: - busco: - description: BUSCO provides measures for quantitative assessment of genome assembly, gene set, and transcriptome completeness based on evolutionarily informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB. + description: BUSCO provides measures for quantitative assessment of genome assembly, + gene set, and transcriptome completeness based on evolutionarily informed expectations + of gene content from near-universal single-copy orthologs selected from OrthoDB. homepage: https://busco.ezlab.org/ documentation: https://busco.ezlab.org/busco_userguide.html tool_dev_url: https://gitlab.com/ezlab/busco doi: "10.1007/978-1-4939-9173-0_14" licence: ["MIT"] + identifier: biotools:busco input: - - short_summary_txt: - type: file - description: One or more short summary txt files from BUSCO - pattern: "short_summary.*.txt" + - - short_summary_txt: + type: file + description: One or more short summary txt files from BUSCO + pattern: "short_summary.*.txt" output: - png: - type: file - description: A summary plot in png format - pattern: "*.png" + - "*.png": + type: file + description: A summary plot in png format + pattern: "*.png" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/bwa/index/meta.yml b/modules/gallvp/bwa/index/meta.yml index 6bbc87a6..4884bca2 100644 --- a/modules/gallvp/bwa/index/meta.yml +++ b/modules/gallvp/bwa/index/meta.yml @@ -14,29 +14,32 @@ tools: documentation: https://bio-bwa.sourceforge.net/bwa.shtml arxiv: arXiv:1303.3997 licence: ["GPL-3.0-or-later"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test', single_end:false ] - - fasta: - type: file - description: Input genome fasta file + - - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Input genome fasta file output: - - meta: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test', single_end:false ] - index: - type: file - description: BWA genome index files - pattern: "*.{amb,ann,bwt,pac,sa}" + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - bwa: + type: file + description: BWA genome index files + pattern: "*.{amb,ann,bwt,pac,sa}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@maxulysse" diff --git a/modules/gallvp/bwa/mem/meta.yml b/modules/gallvp/bwa/mem/meta.yml index b126dd86..37467d29 100644 --- a/modules/gallvp/bwa/mem/meta.yml +++ b/modules/gallvp/bwa/mem/meta.yml @@ -17,55 +17,82 @@ tools: documentation: https://bio-bwa.sourceforge.net/bwa.shtml arxiv: arXiv:1303.3997 licence: ["GPL-3.0-or-later"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - meta2: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test', single_end:false ] - - index: - type: file - description: BWA genome index files - pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" - - fasta: - type: file - description: Reference genome in FASTA format - pattern: "*.{fasta,fa}" - - sort_bam: - type: boolean - description: use samtools sort (true) or samtools view (false) - pattern: "true or false" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: BWA genome index files + pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" + - - meta3: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Reference genome in FASTA format + pattern: "*.{fasta,fa}" + - - sort_bam: + type: boolean + description: use samtools sort (true) or samtools view (false) + pattern: "true or false" output: - bam: - type: file - description: Output BAM file containing read alignments - pattern: "*.{bam}" + - meta: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - "*.bam": + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" - cram: - type: file - description: Output CRAM file containing read alignments - pattern: "*.{cram}" + - meta: + type: file + description: Output CRAM file containing read alignments + pattern: "*.{cram}" + - "*.cram": + type: file + description: Output CRAM file containing read alignments + pattern: "*.{cram}" - csi: - type: file - description: Optional index file for BAM file - pattern: "*.{csi}" + - meta: + type: file + description: Optional index file for BAM file + pattern: "*.{csi}" + - "*.csi": + type: file + description: Optional index file for BAM file + pattern: "*.{csi}" - crai: - type: file - description: Optional index file for CRAM file - pattern: "*.{crai}" + - meta: + type: file + description: Optional index file for CRAM file + pattern: "*.{crai}" + - "*.crai": + type: file + description: Optional index file for CRAM file + pattern: "*.{crai}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@jeremy1805" diff --git a/modules/gallvp/cat/cat/meta.yml b/modules/gallvp/cat/cat/meta.yml index 00a8db0b..81778a06 100644 --- a/modules/gallvp/cat/cat/meta.yml +++ b/modules/gallvp/cat/cat/meta.yml @@ -9,25 +9,32 @@ tools: description: Just concatenation documentation: https://man7.org/linux/man-pages/man1/cat.1.html licence: ["GPL-3.0-or-later"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - files_in: - type: file - description: List of compressed / uncompressed files - pattern: "*" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - files_in: + type: file + description: List of compressed / uncompressed files + pattern: "*" output: - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - file_out: - type: file - description: Concatenated file. Will be gzipped if file_out ends with ".gz" - pattern: "${file_out}" + - meta: + type: file + description: Concatenated file. Will be gzipped if file_out ends with ".gz" + pattern: "${file_out}" + - ${prefix}: + type: file + description: Concatenated file. Will be gzipped if file_out ends with ".gz" + pattern: "${file_out}" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@erikrikarddaniel" - "@FriederikeHanssen" diff --git a/modules/gallvp/custom/relabelfasta/meta.yml b/modules/gallvp/custom/relabelfasta/meta.yml index 7935872a..bf563aad 100644 --- a/modules/gallvp/custom/relabelfasta/meta.yml +++ b/modules/gallvp/custom/relabelfasta/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "custom_relabelfasta" description: | @@ -20,36 +19,38 @@ tools: documentation: "https://docs.python.org/3/" tool_dev_url: "https://github.com/python/cpython" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: Input fasta file - pattern: "*.fasta" - - labels: - type: file - description: | - A TSV file with original (first column) and new ids (second column) - pattern: "*.tsv" - + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: Input fasta file + pattern: "*.fasta" + - - labels: + type: file + description: | + A TSV file with original (first column) and new ids (second column) + pattern: "*.tsv" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - fasta: - type: file - description: Output fasta file - pattern: "*.fasta" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.fasta": + type: file + description: Output fasta file + pattern: "*.fasta" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/custom/restoregffids/meta.yml b/modules/gallvp/custom/restoregffids/meta.yml index 4e42b829..dea75778 100644 --- a/modules/gallvp/custom/restoregffids/meta.yml +++ b/modules/gallvp/custom/restoregffids/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "custom_restoregffids" description: | @@ -22,36 +21,39 @@ tools: documentation: "https://docs.python.org/3/" tool_dev_url: "https://github.com/python/cpython" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" - - ids_tsv: - type: file - description: | - A TSV file with original (first column) and new ids (second column) - if id change was required - pattern: "*.tsv" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" + - - ids_tsv: + type: file + description: | + A TSV file with original (first column) and new ids (second column) + if id change was required + pattern: "*.tsv" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - restored_ids_gff3: - type: file - description: GFF3 file with restored ids - pattern: "*.restored.ids.gff3" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.restored.ids.gff3": + type: file + description: GFF3 file with restored ids + pattern: "*.restored.ids.gff3" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/custom/shortenfastaids/meta.yml b/modules/gallvp/custom/shortenfastaids/meta.yml index 2425810d..bb14b0ae 100644 --- a/modules/gallvp/custom/shortenfastaids/meta.yml +++ b/modules/gallvp/custom/shortenfastaids/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "custom_shortenfastaids" description: | @@ -22,36 +21,45 @@ tools: tool_dev_url: "https://github.com/biopython/biopython" doi: "10.1093/bioinformatics/btp163" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - fasta: - type: file - description: Input fasta file - pattern: "*.{fsa,fa,fasta}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - fasta: + type: file + description: Input fasta file + pattern: "*.{fsa,fa,fasta}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - short_ids_fasta: - type: file - description: Fasta file with shortened ids if id change is required - pattern: "*.{fsa,fa,fasta}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.short.ids.fasta": + type: file + description: Fasta file with shortened ids if id change is required + pattern: "*.{fsa,fa,fasta}" - short_ids_tsv: - type: file - description: | - A TSV file with original (first column) and new ids (second column) - if id change is required - pattern: "*.tsv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.short.ids.tsv": + type: file + description: | + A TSV file with original (first column) and new ids (second column) + if id change is required + pattern: "*.tsv" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/gffread/meta.yml b/modules/gallvp/gffread/meta.yml index c0602820..bebe7f57 100644 --- a/modules/gallvp/gffread/meta.yml +++ b/modules/gallvp/gffread/meta.yml @@ -1,53 +1,73 @@ name: gffread -description: Validate, filter, convert and perform various other operations on GFF files +description: Validate, filter, convert and perform various other operations on GFF + files keywords: - gff - conversion - validation tools: - gffread: - description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more. + description: GFF/GTF utility providing format conversions, region filtering, FASTA + sequence extraction and more. homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread tool_dev_url: https://github.com/gpertea/gffread doi: 10.12688/f1000research.23297.1 licence: ["MIT"] + identifier: biotools:gffread input: - - meta: - type: map - description: | - Groovy Map containing meta data - e.g. [ id:'test' ] - - gff: - type: file - description: A reference file in either the GFF3, GFF2 or GTF format. - pattern: "*.{gff, gtf}" - - fasta: - type: file - description: A multi-fasta file with the genomic sequences - pattern: "*.{fasta,fa,faa,fas,fsa}" + - - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test' ] + - gff: + type: file + description: A reference file in either the GFF3, GFF2 or GTF format. + pattern: "*.{gff, gtf}" + - - fasta: + type: file + description: A multi-fasta file with the genomic sequences + pattern: "*.{fasta,fa,faa,fas,fsa}" output: - - meta: - type: map - description: | - Groovy Map containing meta data - e.g. [ id:'test' ] - gtf: - type: file - description: GTF file resulting from the conversion of the GFF input file if '-T' argument is present - pattern: "*.{gtf}" + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test' ] + - "*.gtf": + type: file + description: GTF file resulting from the conversion of the GFF input file if + '-T' argument is present + pattern: "*.{gtf}" - gffread_gff: - type: file - description: GFF3 file resulting from the conversion of the GFF input file if '-T' argument is absent - pattern: "*.gff3" + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test' ] + - "*.gff3": + type: file + description: GFF3 file resulting from the conversion of the GFF input file if + '-T' argument is absent + pattern: "*.gff3" - gffread_fasta: - type: file - description: Fasta file produced when either of '-w', '-x', '-y' parameters is present - pattern: "*.fasta" + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test' ] + - "*.fasta": + type: file + description: Fasta file produced when either of '-w', '-x', '-y' parameters + is present + pattern: "*.fasta" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@edmundmiller" maintainers: diff --git a/modules/gallvp/gt/gff3/meta.yml b/modules/gallvp/gt/gff3/meta.yml index 5cecd8d0..62c4cbc6 100644 --- a/modules/gallvp/gt/gff3/meta.yml +++ b/modules/gallvp/gt/gff3/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "gt_gff3" -description: "GenomeTools gt-gff3 utility to parse, possibly transform, and output GFF3 files" +description: "GenomeTools gt-gff3 utility to parse, possibly transform, and output + GFF3 files" keywords: - genome - gff3 @@ -14,34 +14,43 @@ tools: tool_dev_url: "https://github.com/genometools/genometools" doi: "10.1109/TCBB.2013.68" licence: ["ISC"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - gt_gff3: - type: file - description: Parsed gff3 file produced only if there is no parsing error - pattern: "*.gt.gff3" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.gt.gff3": + type: file + description: Parsed gff3 file produced only if there is no parsing error + pattern: "*.gt.gff3" - error_log: - type: file - description: Error log if gt-gff3 failed to parse the input gff3 file - pattern: "*.error.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.error.log": + type: file + description: Error log if gt-gff3 failed to parse the input gff3 file + pattern: "*.error.log" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@gallvp" maintainers: diff --git a/modules/gallvp/gt/gff3validator/meta.yml b/modules/gallvp/gt/gff3validator/meta.yml index 3322faf9..98ff256d 100644 --- a/modules/gallvp/gt/gff3validator/meta.yml +++ b/modules/gallvp/gt/gff3validator/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "gt_gff3validator" description: "GenomeTools gt-gff3validator utility to strictly validate a GFF3 file" @@ -15,34 +14,43 @@ tools: tool_dev_url: "https://github.com/genometools/genometools" doi: "10.1109/TCBB.2013.68" licence: ["ISC"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - success_log: - type: file - description: Log file for successful validation - pattern: "*.success.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.success.log": + type: file + description: Log file for successful validation + pattern: "*.success.log" - error_log: - type: file - description: Log file for failed validation - pattern: "*.error.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - "*.error.log": + type: file + description: Log file for failed validation + pattern: "*.error.log" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/gt/stat/meta.yml b/modules/gallvp/gt/stat/meta.yml index fa477f34..3f62f8eb 100644 --- a/modules/gallvp/gt/stat/meta.yml +++ b/modules/gallvp/gt/stat/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "gt_stat" -description: "GenomeTools gt-stat utility to show statistics about features contained in GFF3 files" +description: "GenomeTools gt-stat utility to show statistics about features contained + in GFF3 files" keywords: - genome - gff3 @@ -16,30 +16,33 @@ tools: tool_dev_url: "https://github.com/genometools/genometools" doi: "10.1109/TCBB.2013.68" licence: ["ISC"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - stats: - type: file - description: Stats file in yaml format - pattern: "*.yml" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - ${prefix}.yml: + type: file + description: Stats file in yaml format + pattern: "*.yml" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/ltrfinder/meta.yml b/modules/gallvp/ltrfinder/meta.yml index e3c672b9..547fb67d 100644 --- a/modules/gallvp/ltrfinder/meta.yml +++ b/modules/gallvp/ltrfinder/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "ltrfinder" description: | @@ -19,41 +18,52 @@ tools: tool_dev_url: "https://github.com/oushujun/LTR_FINDER_parallel" doi: "10.1186/s13100-019-0193-0" licence: ["MIT"] + identifier: "" - "LTR_Finder": - description: An efficient program for finding full-length LTR retrotranspsons in genome sequences + description: An efficient program for finding full-length LTR retrotranspsons + in genome sequences homepage: "https://github.com/xzhub/LTR_Finder" documentation: "https://github.com/xzhub/LTR_Finder" tool_dev_url: "https://github.com/xzhub/LTR_Finder" doi: "10.1093/nar/gkm286" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: Genome sequences in fasta format - pattern: "*.{fsa,fa,fasta}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: Genome sequences in fasta format + pattern: "*.{fsa,fa,fasta}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - scn: - type: file - description: Annotation in LTRharvest or LTR_FINDER format - pattern: "*.scn" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.scn": + type: file + description: Annotation in LTRharvest or LTR_FINDER format + pattern: "*.scn" - gff: - type: file - description: Annotation in gff3 format - pattern: "*.gff3" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.gff3": + type: file + description: Annotation in gff3 format + pattern: "*.gff3" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/ltrfinder/tests/main.nf.test b/modules/gallvp/ltrfinder/tests/main.nf.test index c08af00a..4af6bba5 100644 --- a/modules/gallvp/ltrfinder/tests/main.nf.test +++ b/modules/gallvp/ltrfinder/tests/main.nf.test @@ -7,13 +7,13 @@ nextflow_process { tag "modules" tag "modules_gallvp" tag "ltrfinder" - tag "gunzip/main" + tag "gunzip" test("actinidia_chinensis-genome_21_fasta_gz-success") { setup { run('GUNZIP') { - script "../../gunzip/main" + script "../../gunzip/main.nf" process { """ diff --git a/modules/gallvp/ltrharvest/meta.yml b/modules/gallvp/ltrharvest/meta.yml index 256b3ce5..18064183 100644 --- a/modules/gallvp/ltrharvest/meta.yml +++ b/modules/gallvp/ltrharvest/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "ltrharvest" description: | @@ -18,6 +17,7 @@ tools: documentation: "https://github.com/oushujun/EDTA/tree/v2.2.0/bin/LTR_HARVEST_parallel" tool_dev_url: "https://github.com/oushujun/EDTA/tree/v2.2.0/bin/LTR_HARVEST_parallel" licence: ["MIT"] + identifier: "" - "gt": description: "The GenomeTools genome analysis system" homepage: "https://genometools.org/index.html" @@ -25,34 +25,43 @@ tools: tool_dev_url: "https://github.com/genometools/genometools" doi: "10.1109/TCBB.2013.68" licence: ["ISC"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: Input genome fasta - pattern: "*.{fsa,fa,fasta}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: Input genome fasta + pattern: "*.{fsa,fa,fasta}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - gff3: - type: file - description: Predicted LTR candidates in gff3 format - pattern: "*.gff3" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.gff3": + type: file + description: Predicted LTR candidates in gff3 format + pattern: "*.gff3" - scn: - type: file - description: Predicted LTR candidates in scn format - pattern: "*.scn" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.scn": + type: file + description: Predicted LTR candidates in scn format + pattern: "*.scn" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/ltrretriever/lai/meta.yml b/modules/gallvp/ltrretriever/lai/meta.yml index f84cf6ca..56efcccc 100644 --- a/modules/gallvp/ltrretriever/lai/meta.yml +++ b/modules/gallvp/ltrretriever/lai/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "ltrretriever_lai" description: | @@ -20,50 +19,59 @@ tools: tool_dev_url: "https://github.com/oushujun/LTR_retriever" doi: "10.1093/nar/gky730" licence: ["GPL v3"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: The genome file that is used to generate everything - pattern: "*.{fsa,fa,fasta}" - - pass_list: - type: file - description: A list of intact LTR-RTs generated by LTR_retriever - pattern: "*.pass.list" - - annotation_out: - type: file - description: RepeatMasker annotation of all LTR sequences in the genome - pattern: "*.out" - - monoploid_seqs: - type: file - description: | - This parameter is mainly for ployploid genomes. User provides a list of - sequence names that represent a monoploid (1x). LAI will be calculated only - on these sequences if provided. - pattern: "*.txt" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The genome file that is used to generate everything + pattern: "*.{fsa,fa,fasta}" + - - pass_list: + type: file + description: A list of intact LTR-RTs generated by LTR_retriever + pattern: "*.pass.list" + - - annotation_out: + type: file + description: RepeatMasker annotation of all LTR sequences in the genome + pattern: "*.out" + - - monoploid_seqs: + type: file + description: | + This parameter is mainly for ployploid genomes. User provides a list of + sequence names that represent a monoploid (1x). LAI will be calculated only + on these sequences if provided. + pattern: "*.txt" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1', single_end:false ]` - log: - type: file - description: Log from LAI - pattern: "*.LAI.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - "*.LAI.log": + type: file + description: Log from LAI + pattern: "*.LAI.log" - lai_out: - type: file - description: | - Output file from LAI if LAI is able to estimate the index from the inputs - pattern: "*.LAI.out" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - "*.LAI.out": + type: file + description: | + Output file from LAI if LAI is able to estimate the index from the inputs + pattern: "*.LAI.out" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/ltrretriever/ltrretriever/meta.yml b/modules/gallvp/ltrretriever/ltrretriever/meta.yml index a310b04a..9645de2d 100644 --- a/modules/gallvp/ltrretriever/ltrretriever/meta.yml +++ b/modules/gallvp/ltrretriever/ltrretriever/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "ltrretriever_ltrretriever" description: Identifies LTR retrotransposons using LTR_retriever @@ -16,67 +15,104 @@ tools: tool_dev_url: "https://github.com/oushujun/LTR_retriever" doi: "10.1104/pp.17.01310" licence: ["GPL v3"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - genome: - type: file - description: Genomic sequences in fasta format - pattern: "*.{fsa,fa,fasta}" - - harvest: - type: file - description: LTR-RT candidates from GenomeTools ltrharvest in the old tabular format - pattern: "*.tabout" - - finder: - type: file - description: LTR-RT candidates from LTR_FINDER - pattern: "*.scn" - - mgescan: - type: file - description: LTR-RT candidates from MGEScan_LTR - pattern: "*.out" - - non_tgca: - type: file - description: Non-canonical LTR-RT candidates from GenomeTools ltrharvest in the old tabular format - pattern: "*.tabout" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - genome: + type: file + description: Genomic sequences in fasta format + pattern: "*.{fsa,fa,fasta}" + - - harvest: + type: file + description: LTR-RT candidates from GenomeTools ltrharvest in the old tabular + format + pattern: "*.tabout" + - - finder: + type: file + description: LTR-RT candidates from LTR_FINDER + pattern: "*.scn" + - - mgescan: + type: file + description: LTR-RT candidates from MGEScan_LTR + pattern: "*.out" + - - non_tgca: + type: file + description: Non-canonical LTR-RT candidates from GenomeTools ltrharvest in + the old tabular format + pattern: "*.tabout" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - log: - type: file - description: Output log from LTR_retriever - pattern: "*.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.log": + type: file + description: Output log from LTR_retriever + pattern: "*.log" - pass_list: - type: file - description: Intact LTR-RTs with coordinate and structural information in summary table format - pattern: "*.pass.list" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - ${prefix}.pass.list: + type: file + description: Intact LTR-RTs with coordinate and structural information in summary + table format + pattern: "*.pass.list" - pass_list_gff: - type: file - description: Intact LTR-RTs with coordinate and structural information in gff3 format - pattern: "*.pass.list.gff3" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.pass.list.gff3": + type: file + description: Intact LTR-RTs with coordinate and structural information in gff3 + format + pattern: "*.pass.list.gff3" - ltrlib: - type: file - description: All non-redundant LTR-RTs - pattern: "*.LTRlib.fa" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.LTRlib.fa": + type: file + description: All non-redundant LTR-RTs + pattern: "*.LTRlib.fa" - annotation_out: - type: file - description: Whole-genome LTR-RT annotation by the non-redundant library - pattern: "*.out" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - ${prefix}.out: + type: file + description: Whole-genome LTR-RT annotation by the non-redundant library + pattern: "*.out" - annotation_gff: - type: file - description: Whole-genome LTR-RT annotation by the non-redundant library in gff3 format - pattern: "*.out.gff3" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.out.gff3": + type: file + description: Whole-genome LTR-RT annotation by the non-redundant library in + gff3 format + pattern: "*.out.gff3" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/ltrretriever/ltrretriever/tests/main.nf.test b/modules/gallvp/ltrretriever/ltrretriever/tests/main.nf.test index 91b1a104..52dd7ac0 100644 --- a/modules/gallvp/ltrretriever/ltrretriever/tests/main.nf.test +++ b/modules/gallvp/ltrretriever/ltrretriever/tests/main.nf.test @@ -9,7 +9,7 @@ nextflow_process { tag "modules_gallvp" tag "ltrretriever" tag "ltrretriever/ltrretriever" - tag "gunzip/main" + tag "gunzip" tag "ltrharvest" tag "ltrfinder" tag "cat/cat" @@ -19,7 +19,7 @@ nextflow_process { setup { run("LTRHARVEST") { - script "../../../ltrharvest" + script "../../../ltrharvest/main.nf" process { """ @@ -32,7 +32,7 @@ nextflow_process { } run("LTRFINDER") { - script "../../../ltrfinder" + script "../../../ltrfinder/main.nf" process { """ @@ -45,7 +45,7 @@ nextflow_process { } run("CAT_CAT") { - script "../../../cat/cat" + script "../../../cat/cat/main.nf" process { """ @@ -85,7 +85,7 @@ nextflow_process { setup { run('GUNZIP') { - script "../../../gunzip/main" + script "../../../gunzip/main.nf" process { """ @@ -98,7 +98,7 @@ nextflow_process { } run("LTRHARVEST") { - script "../../../ltrharvest" + script "../../../ltrharvest/main.nf" process { """ @@ -108,7 +108,7 @@ nextflow_process { } run("LTRFINDER") { - script "../../../ltrfinder" + script "../../../ltrfinder/main.nf" process { """ @@ -118,7 +118,7 @@ nextflow_process { } run("CAT_CAT") { - script "../../../cat/cat" + script "../../../cat/cat/main.nf" process { """ diff --git a/modules/gallvp/plotsr/meta.yml b/modules/gallvp/plotsr/meta.yml index c728b683..c2bc2993 100644 --- a/modules/gallvp/plotsr/meta.yml +++ b/modules/gallvp/plotsr/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "plotsr" -description: Plotsr generates high-quality visualisation of synteny and structural rearrangements between multiple genomes. +description: Plotsr generates high-quality visualisation of synteny and structural + rearrangements between multiple genomes. keywords: - genomics - synteny @@ -15,64 +15,67 @@ tools: tool_dev_url: "https://github.com/schneebergerlab/plotsr" doi: "10.1093/bioinformatics/btac196" licence: ["MIT"] + identifier: biotools:plotsr input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - syri: - type: file - description: Structural annotation mappings (syri.out) identified by SyRI - pattern: "*syri.out" - - fastas: - type: list - description: Fasta files in the sequence specified by the `genomes` file - pattern: "*.{fasta,fa,fsa,faa}" - - genomes: - type: string - description: | - String containing the genomes.txt file contents including the header, but excluding the path to genome fasta files. - The path to staged genome files is automatically added by the process script. For example, see the included nf-test. - pattern: "*.txt" - - bedpe: - type: file - description: Structural annotation mappings in BEDPE format - pattern: "*.bedpe" - - markers: - type: file - description: File containing path to markers - pattern: "*.bed" - - tracks: - type: file - description: File listing paths and details for all tracks to be plotted - pattern: "*.txt" - - chrord: - type: file - description: | - File containing reference (first genome) chromosome IDs in the order in which they are to be plotted. - File requires one chromosome ID per line. Not compatible with --chr - pattern: "*.txt" - - chrname: - type: file - description: | - File containing reference (first genome) chromosome names to be used in the plot. - File needs to be a TSV with the chromosome ID in first column and chromosome name in the second. - pattern: "*.txt" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - syri: + type: file + description: Structural annotation mappings (syri.out) identified by SyRI + pattern: "*syri.out" + - - fastas: + type: list + description: Fasta files in the sequence specified by the `genomes` file + pattern: "*.{fasta,fa,fsa,faa}" + - - genomes: + type: string + description: | + String containing the genomes.txt file contents including the header, but excluding the path to genome fasta files. + The path to staged genome files is automatically added by the process script. For example, see the included nf-test. + pattern: "*.txt" + - - bedpe: + type: file + description: Structural annotation mappings in BEDPE format + pattern: "*.bedpe" + - - markers: + type: file + description: File containing path to markers + pattern: "*.bed" + - - tracks: + type: file + description: File listing paths and details for all tracks to be plotted + pattern: "*.txt" + - - chrord: + type: file + description: | + File containing reference (first genome) chromosome IDs in the order in which they are to be plotted. + File requires one chromosome ID per line. Not compatible with --chr + pattern: "*.txt" + - - chrname: + type: file + description: | + File containing reference (first genome) chromosome names to be used in the plot. + File needs to be a TSV with the chromosome ID in first column and chromosome name in the second. + pattern: "*.txt" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - png: - type: file - description: Synteny plot - pattern: "*.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.png": + type: file + description: Synteny plot + pattern: "*.png" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/samblaster/meta.yml b/modules/gallvp/samblaster/meta.yml index 5c1e5a97..5faf3a6c 100644 --- a/modules/gallvp/samblaster/meta.yml +++ b/modules/gallvp/samblaster/meta.yml @@ -23,30 +23,33 @@ tools: tool_dev_url: https://github.com/GregoryFaust/samblaster doi: "10.1093/bioinformatics/btu314" licence: ["MIT"] + identifier: biotools:samblaster input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.bam" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.bam" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - bam: - type: file - description: Tagged or filtered BAM file - pattern: "*.bam" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.bam": + type: file + description: Tagged or filtered BAM file + pattern: "*.bam" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@lescai" maintainers: diff --git a/modules/gallvp/samtools/faidx/environment.yml b/modules/gallvp/samtools/faidx/environment.yml index b98cbb99..2bcd47ee 100644 --- a/modules/gallvp/samtools/faidx/environment.yml +++ b/modules/gallvp/samtools/faidx/environment.yml @@ -3,5 +3,5 @@ channels: - bioconda dependencies: - - bioconda::htslib=1.20 - - bioconda::samtools=1.20 + - bioconda::htslib=1.21 + - bioconda::samtools=1.21 diff --git a/modules/gallvp/samtools/faidx/main.nf b/modules/gallvp/samtools/faidx/main.nf index bdcdbc95..28c0a81c 100644 --- a/modules/gallvp/samtools/faidx/main.nf +++ b/modules/gallvp/samtools/faidx/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : - 'biocontainers/samtools:1.20--h50ea8bc_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' : + 'biocontainers/samtools:1.21--h50ea8bc_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/gallvp/samtools/faidx/meta.yml b/modules/gallvp/samtools/faidx/meta.yml index f3c25de2..6721b2cb 100644 --- a/modules/gallvp/samtools/faidx/meta.yml +++ b/modules/gallvp/samtools/faidx/meta.yml @@ -14,47 +14,62 @@ tools: documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] + identifier: biotools:samtools input: - - meta: - type: map - description: | - Groovy Map containing reference information - e.g. [ id:'test' ] - - fasta: - type: file - description: FASTA file - pattern: "*.{fa,fasta}" - - meta2: - type: map - description: | - Groovy Map containing reference information - e.g. [ id:'test' ] - - fai: - type: file - description: FASTA index file - pattern: "*.{fai}" + - - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] + - fasta: + type: file + description: FASTA file + pattern: "*.{fa,fasta}" + - - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] + - fai: + type: file + description: FASTA index file + pattern: "*.{fai}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - fa: - type: file - description: FASTA file - pattern: "*.{fa}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.{fa,fasta}": + type: file + description: FASTA file + pattern: "*.{fa}" - fai: - type: file - description: FASTA index file - pattern: "*.{fai}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.fai": + type: file + description: FASTA index file + pattern: "*.{fai}" - gzi: - type: file - description: Optional gzip index file for compressed inputs - pattern: "*.gzi" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.gzi": + type: file + description: Optional gzip index file for compressed inputs + pattern: "*.gzi" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@ewels" diff --git a/modules/gallvp/samtools/faidx/tests/main.nf.test.snap b/modules/gallvp/samtools/faidx/tests/main.nf.test.snap index 3223b72b..1bbb3ec2 100644 --- a/modules/gallvp/samtools/faidx/tests/main.nf.test.snap +++ b/modules/gallvp/samtools/faidx/tests/main.nf.test.snap @@ -18,7 +18,7 @@ ], "3": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ], "fa": [ @@ -36,15 +36,15 @@ ], "versions": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-05-28T15:42:14.779784761" + "timestamp": "2024-09-16T07:57:47.450887871" }, "test_samtools_faidx_bgzip": { "content": [ @@ -71,7 +71,7 @@ ] ], "3": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ], "fa": [ @@ -95,15 +95,15 @@ ] ], "versions": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-05-28T15:42:20.256633877" + "timestamp": "2024-09-16T07:58:04.804905659" }, "test_samtools_faidx_fasta": { "content": [ @@ -124,7 +124,7 @@ ], "3": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ], "fa": [ [ @@ -142,15 +142,15 @@ ], "versions": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-05-28T15:42:25.632577273" + "timestamp": "2024-09-16T07:58:23.831268154" }, "test_samtools_faidx_stub_fasta": { "content": [ @@ -171,7 +171,7 @@ ], "3": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ], "fa": [ [ @@ -189,15 +189,15 @@ ], "versions": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-05-28T15:42:31.058424849" + "timestamp": "2024-09-16T07:58:35.600243706" }, "test_samtools_faidx_stub_fai": { "content": [ @@ -218,7 +218,7 @@ ], "3": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ], "fa": [ @@ -236,14 +236,14 @@ ], "versions": [ - "versions.yml:md5,2db78952923a61e05d50b95518b21856" + "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-05-28T15:42:36.479929617" + "timestamp": "2024-09-16T07:58:54.705460167" } } \ No newline at end of file diff --git a/modules/gallvp/seqkit/seq/meta.yml b/modules/gallvp/seqkit/seq/meta.yml index 8d4e2b16..7d32aba5 100644 --- a/modules/gallvp/seqkit/seq/meta.yml +++ b/modules/gallvp/seqkit/seq/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "seqkit_seq" -description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...) +description: Transforms sequences (extract ID, filter by length, remove gaps, reverse + complement...) keywords: - genomics - fasta @@ -18,30 +18,33 @@ tools: tool_dev_url: "https://github.com/shenwei356/seqkit" doi: "10.1371/journal.pone.0163962" licence: ["MIT"] + identifier: biotools:seqkit input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Input fasta/fastq file - pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - fastx: - type: file - description: Output fasta/fastq file - pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - ${prefix}.*: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/gallvp/syri/meta.yml b/modules/gallvp/syri/meta.yml index d7801532..9699bacd 100644 --- a/modules/gallvp/syri/meta.yml +++ b/modules/gallvp/syri/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "syri" -description: Syri compares alignments between two chromosome-level assemblies and identifies synteny and structural rearrangements. +description: Syri compares alignments between two chromosome-level assemblies and + identifies synteny and structural rearrangements. keywords: - genomics - synteny @@ -15,49 +15,57 @@ tools: tool_dev_url: "https://github.com/schneebergerlab/syri" doi: "10.1186/s13059-019-1911-0" licence: ["MIT License"] + identifier: biotools:SyRI input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - infile: - type: file - description: File containing alignment coordinates - pattern: "*.{table, sam, bam, paf}" - - query_fasta: - type: file - description: Query genome for the alignments - pattern: "*.fasta" - - reference_fasta: - type: file - description: Reference genome for the alignments - pattern: "*.fasta" - - file_type: - type: string - description: | - Input file type which is one of T: Table, S: SAM, B: BAM, P: PAF - + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - infile: + type: file + description: File containing alignment coordinates + pattern: "*.{table, sam, bam, paf}" + - - query_fasta: + type: file + description: Query genome for the alignments + pattern: "*.fasta" + - - reference_fasta: + type: file + description: Reference genome for the alignments + pattern: "*.fasta" + - - file_type: + type: string + description: | + Input file type which is one of T: Table, S: SAM, B: BAM, P: PAF output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - syri: - type: file - description: Syri output file - pattern: "*syri.out" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*syri.out": + type: file + description: Syri output file + pattern: "*syri.out" - error: - type: file - description: | - Error log if syri fails. This error log enables the pipeline to detect if syri has failed due to one of its - known limitations and pass the information to the user in a user-friendly manner such as a HTML report - pattern: "*.error.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.error.log": + type: file + description: | + Error log if syri fails. This error log enables the pipeline to detect if syri has failed due to one of its + known limitations and pass the information to the user in a user-friendly manner such as a HTML report + pattern: "*.error.log" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/custom/sratoolsncbisettings/meta.yml b/modules/nf-core/custom/sratoolsncbisettings/meta.yml index 46a6cd32..2938a35d 100644 --- a/modules/nf-core/custom/sratoolsncbisettings/meta.yml +++ b/modules/nf-core/custom/sratoolsncbisettings/meta.yml @@ -1,5 +1,6 @@ name: "custom_sratoolsncbisettings" -description: Test for the presence of suitable NCBI settings or create them on the fly. +description: Test for the presence of suitable NCBI settings or create them on the + fly. keywords: - NCBI - settings @@ -13,15 +14,18 @@ tools: documentation: https://github.com/ncbi/sra-tools/wiki tool_dev_url: https://github.com/ncbi/sra-tools licence: ["Public Domain"] + identifier: "" output: - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - ncbi_settings: - type: file - description: An NCBI user settings file. - pattern: "*.mkfg" + - "*.mkfg": + type: file + description: An NCBI user settings file. + pattern: "*.mkfg" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@Midnighter" maintainers: diff --git a/modules/nf-core/fastavalidator/meta.yml b/modules/nf-core/fastavalidator/meta.yml index c5c4371c..94198e62 100644 --- a/modules/nf-core/fastavalidator/meta.yml +++ b/modules/nf-core/fastavalidator/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "fastavalidator" description: | @@ -19,34 +18,43 @@ tools: tool_dev_url: "https://github.com/linsalrob/py_fasta_validator" doi: "10.5281/zenodo.5002710" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing file information - e.g. [ id:'test' ] - - fasta: - type: file - description: Input fasta file - pattern: "*.fasta" + - - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - fasta: + type: file + description: Input fasta file + pattern: "*.fasta" output: - - meta: - type: map - description: | - Groovy Map containing file information - e.g. [ id:'test' ] - success_log: - type: file - description: Log file for successful validation - pattern: "*.success.log" + - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - "*.success.log": + type: file + description: Log file for successful validation + pattern: "*.success.log" - error_log: - type: file - description: Log file for failed validation - pattern: "*.error.log" + - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - "*.error.log": + type: file + description: Log file for failed validation + pattern: "*.error.log" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@gallvp" maintainers: diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml index 8dfecc18..159404d0 100644 --- a/modules/nf-core/fastp/meta.yml +++ b/modules/nf-core/fastp/meta.yml @@ -11,66 +11,100 @@ tools: documentation: https://github.com/OpenGene/fastp doi: 10.1093/bioinformatics/bty560 licence: ["MIT"] + identifier: biotools:fastp input: - - meta: - type: map - description: | - Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads. - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. If you wish to run interleaved paired-end data, supply as single-end data - but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. - - adapter_fasta: - type: file - description: File in FASTA format containing possible adapters to remove. - pattern: "*.{fasta,fna,fas,fa}" - - discard_trimmed_pass: - type: boolean - description: Specify true to not write any reads that pass trimming thresholds. | - This can be used to use fastp for the output report only. - - save_trimmed_fail: - type: boolean - description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` - - save_merged: - type: boolean - description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz` + - - meta: + type: map + description: | + Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads. + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. If you wish to run interleaved paired-end data, supply as single-end data + but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - - adapter_fasta: + type: file + description: File in FASTA format containing possible adapters to remove. + pattern: "*.{fasta,fna,fas,fa}" + - - discard_trimmed_pass: + type: boolean + description: Specify true to not write any reads that pass trimming thresholds. + | This can be used to use fastp for the output report only. + - - save_trimmed_fail: + type: boolean + description: Specify true to save files that failed to pass trimming thresholds + ending in `*.fail.fastq.gz` + - - save_merged: + type: boolean + description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz` output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - reads: - type: file - description: The trimmed/modified/unmerged fastq reads - pattern: "*fastp.fastq.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.fastp.fastq.gz": + type: file + description: The trimmed/modified/unmerged fastq reads + pattern: "*fastp.fastq.gz" - json: - type: file - description: Results in JSON format - pattern: "*.json" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.json": + type: file + description: Results in JSON format + pattern: "*.json" - html: - type: file - description: Results in HTML format - pattern: "*.html" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.html": + type: file + description: Results in HTML format + pattern: "*.html" - log: - type: file - description: fastq log file - pattern: "*.log" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.log": + type: file + description: fastq log file + pattern: "*.log" - reads_fail: - type: file - description: Reads the failed the preprocessing - pattern: "*fail.fastq.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.fail.fastq.gz": + type: file + description: Reads the failed the preprocessing + pattern: "*fail.fastq.gz" - reads_merged: - type: file - description: Reads that were successfully merged - pattern: "*.{merged.fastq.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.merged.fastq.gz": + type: file + description: Reads that were successfully merged + pattern: "*.{merged.fastq.gz}" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@kevinmenden" diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index ee5507e0..4827da7a 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -16,35 +16,44 @@ tools: homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ licence: ["GPL-2.0-only"] + identifier: biotools:fastqc input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.html": + type: file + description: FastQC report + pattern: "*_{fastqc.html}" - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.zip": + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@grst" diff --git a/modules/nf-core/fcs/fcsadaptor/meta.yml b/modules/nf-core/fcs/fcsadaptor/meta.yml index 54fca1bb..83cae5b7 100644 --- a/modules/nf-core/fcs/fcsadaptor/meta.yml +++ b/modules/nf-core/fcs/fcsadaptor/meta.yml @@ -18,45 +18,72 @@ tools: documentation: "https://github.com/ncbi/fcs/wiki/FCS-adaptor" tool_dev_url: "https://github.com/ncbi/fcs" licence: ["United States Government Work"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - assembly: - type: file - description: assembly fasta file + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - assembly: + type: file + description: assembly fasta file output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - cleaned_assembly: - type: file - description: Cleaned assembly in fasta format - pattern: "*.{cleaned_sequences.fa.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.cleaned_sequences.fa.gz": + type: file + description: Cleaned assembly in fasta format + pattern: "*.{cleaned_sequences.fa.gz}" - adaptor_report: - type: file - description: Report of identified adaptors - pattern: "*.{fcs_adaptor_report.txt}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.fcs_adaptor_report.txt": + type: file + description: Report of identified adaptors + pattern: "*.{fcs_adaptor_report.txt}" - log: - type: file - description: Log file - pattern: "*.{fcs_adaptor.log}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.fcs_adaptor.log": + type: file + description: Log file + pattern: "*.{fcs_adaptor.log}" - pipeline_args: - type: file - description: Run arguments - pattern: "*.{pipeline_args.yaml}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.pipeline_args.yaml": + type: file + description: Run arguments + pattern: "*.{pipeline_args.yaml}" - skipped_trims: - type: file - description: Skipped trim information - pattern: "*.{skipped_trims.jsonl}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.skipped_trims.jsonl": + type: file + description: Skipped trim information + pattern: "*.{skipped_trims.jsonl}" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@d4straub" maintainers: diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml index f32973a0..9066c035 100644 --- a/modules/nf-core/gunzip/meta.yml +++ b/modules/nf-core/gunzip/meta.yml @@ -10,25 +10,32 @@ tools: gzip is a file format and a software application used for file compression and decompression. documentation: https://www.gnu.org/software/gzip/manual/gzip.html licence: ["GPL-3.0-or-later"] + identifier: "" input: - - meta: - type: map - description: | - Optional groovy Map containing meta information - e.g. [ id:'test', single_end:false ] - - archive: - type: file - description: File to be compressed/uncompressed - pattern: "*.*" + - - meta: + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be compressed/uncompressed + pattern: "*.*" output: - gunzip: - type: file - description: Compressed/uncompressed file - pattern: "*.*" + - meta: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - $gunzip: + type: file + description: Compressed/uncompressed file + pattern: "*.*" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh" diff --git a/modules/nf-core/merqury/hapmers/meta.yml b/modules/nf-core/merqury/hapmers/meta.yml index 6693f540..2c37d3ac 100644 --- a/modules/nf-core/merqury/hapmers/meta.yml +++ b/modules/nf-core/merqury/hapmers/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "merqury_hapmers" description: A script to generate hap-mer dbs for trios @@ -13,57 +12,85 @@ tools: tool_dev_url: "https://github.com/marbl/merqury" doi: "10.1186/s13059-020-02134-9" licence: ["United States Government Work"] + identifier: biotools:merqury input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - child_meryl: - type: directory - description: Childs' k-mers (all, from WGS reads) - pattern: "*.meryl" - - maternal_meryl: - type: directory - description: Haplotype1 k-mers (all, ex. maternal) - pattern: "*.meryl" - - paternal_meryl: - type: directory - description: Haplotype2 k-mers (all, ex. paternal) - pattern: "*.meryl" - + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - child_meryl: + type: directory + description: Childs' k-mers (all, from WGS reads) + pattern: "*.meryl" + - - maternal_meryl: + type: directory + description: Haplotype1 k-mers (all, ex. maternal) + pattern: "*.meryl" + - - paternal_meryl: + type: directory + description: Haplotype2 k-mers (all, ex. paternal) + pattern: "*.meryl" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - mat_hapmer_meryl: - type: directory - description: Inherited maternal hap-mer dbs - pattern: "*_mat.hapmer.meryl" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*_mat.hapmer.meryl": + type: directory + description: Inherited maternal hap-mer dbs + pattern: "*_mat.hapmer.meryl" - pat_hapmer_meryl: - type: directory - description: Inherited paternal hap-mer dbs - pattern: "*_pat.hapmer.meryl" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*_pat.hapmer.meryl": + type: directory + description: Inherited paternal hap-mer dbs + pattern: "*_pat.hapmer.meryl" - inherited_hapmers_fl_png: - type: file - description: k-mer distribution of the inherited dbs and cutoffs used to generate hap-mer dbs - pattern: "*_inherited_hapmers.fl.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*_inherited_hapmers.fl.png": + type: file + description: k-mer distribution of the inherited dbs and cutoffs used to generate + hap-mer dbs + pattern: "*_inherited_hapmers.fl.png" - inherited_hapmers_ln_png: - type: file - description: k-mer distribution of the inherited dbs and cutoffs used to generate hap-mer dbs - pattern: "*_inherited_hapmers.ln.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*_inherited_hapmers.ln.png": + type: file + description: k-mer distribution of the inherited dbs and cutoffs used to generate + hap-mer dbs + pattern: "*_inherited_hapmers.ln.png" - inherited_hapmers_st_png: - type: file - description: k-mer distribution of the inherited dbs and cutoffs used to generate hap-mer dbs - pattern: "*_inherited_hapmers.st.png" - + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*_inherited_hapmers.st.png": + type: file + description: k-mer distribution of the inherited dbs and cutoffs used to generate + hap-mer dbs + pattern: "*_inherited_hapmers.st.png" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/merqury/merqury/meta.yml b/modules/nf-core/merqury/merqury/meta.yml index 19cb11b3..7e8d875a 100644 --- a/modules/nf-core/merqury/merqury/meta.yml +++ b/modules/nf-core/merqury/merqury/meta.yml @@ -10,92 +10,187 @@ tools: tool_dev_url: "https://github.com/marbl/merqury" doi: "10.1186/s13059-020-02134-9" licence: ["PUBLIC DOMAIN"] + identifier: biotools:merqury input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - meryl_db: - type: file - description: "Meryl read database" - - assembly: - type: file - description: FASTA assembly file + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - meryl_db: + type: file + description: "Meryl read database" + - assembly: + type: file + description: FASTA assembly file output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - assembly_only_kmers_bed: - type: file - description: "The positions of the k-mers found only in an assembly for further investigation in .bed" - pattern: "*_only.bed" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*_only.bed": + type: file + description: "The positions of the k-mers found only in an assembly for further + investigation in .bed" + pattern: "*_only.bed" - assembly_only_kmers_wig: - type: file - description: "The positions of the k-mers found only in an assembly for further investigation in .wig" - pattern: "*_only.wig" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*_only.wig": + type: file + description: "The positions of the k-mers found only in an assembly for further + investigation in .wig" + pattern: "*_only.wig" - stats: - type: file - description: Assembly statistics file - pattern: "*.completeness.stats" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.completeness.stats": + type: file + description: Assembly statistics file + pattern: "*.completeness.stats" - dist_hist: - type: file - description: Histogram - pattern: "*.dist_only.hist" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.dist_only.hist": + type: file + description: Histogram + pattern: "*.dist_only.hist" - spectra_cn_fl_png: - type: file - description: "Unstacked copy number spectra filled plot in PNG format" - pattern: "*.spectra-cn.fl.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-cn.fl.png": + type: file + description: "Unstacked copy number spectra filled plot in PNG format" + pattern: "*.spectra-cn.fl.png" + - spectra_cn_hist: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-cn.hist": + type: file + description: "Copy number spectra histogram" + pattern: "*.spectra-cn.hist" - spectra_cn_ln_png: - type: file - description: "Unstacked copy number spectra line plot in PNG format" - pattern: "*.spectra-cn.ln.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-cn.ln.png": + type: file + description: "Unstacked copy number spectra line plot in PNG format" + pattern: "*.spectra-cn.ln.png" - spectra_cn_st_png: - type: file - description: "Stacked copy number spectra line plot in PNG format" - pattern: "*.spectra-cn.st.png" - - spectra_cn_hist: - type: file - description: "Copy number spectra histogram" - pattern: "*.spectra-cn.hist" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-cn.st.png": + type: file + description: "Stacked copy number spectra line plot in PNG format" + pattern: "*.spectra-cn.st.png" - spectra_asm_fl_png: - type: file - description: "Unstacked assembly spectra filled plot in PNG format" - pattern: "*.spectra-asm.fl.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-asm.fl.png": + type: file + description: "Unstacked assembly spectra filled plot in PNG format" + pattern: "*.spectra-asm.fl.png" + - spectra_asm_hist: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-asm.hist": + type: file + description: "Assembly spectra histogram" + pattern: "*.spectra-asm.hist" - spectra_asm_ln_png: - type: file - description: "Unstacked assembly spectra line plot in PNG format" - pattern: "*.spectra-asm.ln.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-asm.ln.png": + type: file + description: "Unstacked assembly spectra line plot in PNG format" + pattern: "*.spectra-asm.ln.png" - spectra_asm_st_png: - type: file - description: "Stacked assembly spectra line plot in PNG format" - pattern: "*.spectra-asm.st.png" - - spectra_asm_hist: - type: file - description: "Assembly spectra histogram" - pattern: "*.spectra-asm.hist" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.spectra-asm.st.png": + type: file + description: "Stacked assembly spectra line plot in PNG format" + pattern: "*.spectra-asm.st.png" - assembly_qv: - type: file - description: "Assembly consensus quality estimation" - pattern: "*.qv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - ${prefix}.qv: + type: file + description: "Assembly consensus quality estimation" + pattern: "*.qv" - scaffold_qv: - type: file - description: "Scaffold consensus quality estimation" - pattern: "*.qv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - ${prefix}.*.qv: + type: file + description: "Scaffold consensus quality estimation" + pattern: "*.qv" - read_ploidy: - type: file - description: "Ploidy estimate from read k-mer database" - pattern: "*.hist.ploidy" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.hist.ploidy": + type: file + description: "Ploidy estimate from read k-mer database" + pattern: "*.hist.ploidy" - hapmers_blob_png: - type: file - description: "Hap-mer blob plot" - pattern: "*.hapmers.blob.png" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.hapmers.blob.png": + type: file + description: "Hap-mer blob plot" + pattern: "*.hapmers.blob.png" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@mahesh-panchal" maintainers: diff --git a/modules/nf-core/meryl/count/meta.yml b/modules/nf-core/meryl/count/meta.yml index 809a32fe..a110a610 100644 --- a/modules/nf-core/meryl/count/meta.yml +++ b/modules/nf-core/meryl/count/meta.yml @@ -11,34 +11,37 @@ tools: documentation: "https://meryl.readthedocs.io/en/latest/quick-start.html" tool_dev_url: "https://github.com/marbl/meryl" licence: ["GPL"] + identifier: biotools:meryl input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - kvalue: - type: integer - description: An integer value of k to use as the k-mer value. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - - kvalue: + type: integer + description: An integer value of k to use as the k-mer value. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - meryl_db: - type: directory - description: A Meryl k-mer database - pattern: "*.meryl" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.meryl": + type: directory + description: A Meryl k-mer database + pattern: "*.meryl" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@mahesh-panchal" maintainers: diff --git a/modules/nf-core/meryl/unionsum/meta.yml b/modules/nf-core/meryl/unionsum/meta.yml index 77d0784c..e9e13051 100644 --- a/modules/nf-core/meryl/unionsum/meta.yml +++ b/modules/nf-core/meryl/unionsum/meta.yml @@ -11,32 +11,35 @@ tools: documentation: "https://meryl.readthedocs.io/en/latest/quick-start.html" tool_dev_url: "https://github.com/marbl/meryl" licence: ["GPL"] + identifier: biotools:meryl input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - meryl_dbs: - type: directory - description: Meryl k-mer databases - - kvalue: - type: integer - description: An integer value of k to use as the k-mer value. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - meryl_dbs: + type: directory + description: Meryl k-mer databases + - - kvalue: + type: integer + description: An integer value of k to use as the k-mer value. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - meryl_db: - type: directory - description: A Meryl k-mer database that is the union sum of the input databases - pattern: "*.unionsum.meryl" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.unionsum.meryl": + type: directory + description: A Meryl k-mer database that is the union sum of the input databases + pattern: "*.unionsum.meryl" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@mahesh-panchal" maintainers: diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml index 8996f881..a4cfc891 100644 --- a/modules/nf-core/minimap2/align/meta.yml +++ b/modules/nf-core/minimap2/align/meta.yml @@ -14,62 +14,77 @@ tools: homepage: https://github.com/lh3/minimap2 documentation: https://github.com/lh3/minimap2#uguide licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FASTA or FASTQ files of size 1 and 2 for single-end - and paired-end data, respectively. - - meta2: - type: map - description: | - Groovy Map containing reference information - e.g. [ id:'test_ref'] - - reference: - type: file - description: | - Reference database in FASTA format. - - bam_format: - type: boolean - description: Specify that output should be in BAM format - - bam_index_extension: - type: string - description: BAM alignment index extension (e.g. "bai") - - cigar_paf_format: - type: boolean - description: Specify that output CIGAR should be in PAF format - - cigar_bam: - type: boolean - description: | - Write CIGAR with >65535 ops at the CG tag. This is recommended when - doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations) + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FASTA or FASTQ files of size 1 and 2 for single-end + and paired-end data, respectively. + - - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test_ref'] + - reference: + type: file + description: | + Reference database in FASTA format. + - - bam_format: + type: boolean + description: Specify that output should be in BAM format + - - bam_index_extension: + type: string + description: BAM alignment index extension (e.g. "bai") + - - cigar_paf_format: + type: boolean + description: Specify that output CIGAR should be in PAF format + - - cigar_bam: + type: boolean + description: | + Write CIGAR with >65535 ops at the CG tag. This is recommended when + doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations) output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - paf: - type: file - description: Alignment in PAF format - pattern: "*.paf" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.paf": + type: file + description: Alignment in PAF format + pattern: "*.paf" - bam: - type: file - description: Alignment in BAM format - pattern: "*.bam" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.bam": + type: file + description: Alignment in BAM format + pattern: "*.bam" - index: - type: file - description: BAM alignment index - pattern: "*.bam.*" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.bam.${bam_index_extension}": + type: file + description: BAM alignment index + pattern: "*.bam.*" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@heuermh" - "@sofstam" diff --git a/modules/nf-core/seqkit/rmdup/meta.yml b/modules/nf-core/seqkit/rmdup/meta.yml index d0addd45..22e29c11 100644 --- a/modules/nf-core/seqkit/rmdup/meta.yml +++ b/modules/nf-core/seqkit/rmdup/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "seqkit_rmdup" -description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...) +description: Transforms sequences (extract ID, filter by length, remove gaps, reverse + complement...) keywords: - genomics - fasta @@ -16,34 +16,43 @@ tools: tool_dev_url: "https://github.com/shenwei356/seqkit" doi: "10.1371/journal.pone.0163962" licence: ["MIT"] + identifier: biotools:seqkit input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Input fasta/fastq file - pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - fastx: - type: file - description: Output fasta/fastq file - pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - ${prefix}.${extension}: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" - log: - type: file - description: Log containing information regarding removed duplicates - pattern: "*.log" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.log": + type: file + description: Log containing information regarding removed duplicates + pattern: "*.log" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/seqkit/seq/meta.yml b/modules/nf-core/seqkit/seq/meta.yml index 8d4e2b16..7d32aba5 100644 --- a/modules/nf-core/seqkit/seq/meta.yml +++ b/modules/nf-core/seqkit/seq/meta.yml @@ -1,7 +1,7 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "seqkit_seq" -description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...) +description: Transforms sequences (extract ID, filter by length, remove gaps, reverse + complement...) keywords: - genomics - fasta @@ -18,30 +18,33 @@ tools: tool_dev_url: "https://github.com/shenwei356/seqkit" doi: "10.1371/journal.pone.0163962" licence: ["MIT"] + identifier: biotools:seqkit input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Input fasta/fastq file - pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - fastx: - type: file - description: Output fasta/fastq file - pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - ${prefix}.*: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/seqkit/sort/meta.yml b/modules/nf-core/seqkit/sort/meta.yml index 2e61ce15..157ff85c 100644 --- a/modules/nf-core/seqkit/sort/meta.yml +++ b/modules/nf-core/seqkit/sort/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "seqkit_sort" description: Sorts sequences by id/name/sequence/length @@ -15,30 +14,33 @@ tools: tool_dev_url: "https://github.com/shenwei356/seqkit" doi: "10.1371/journal.pone.0163962" licence: ["MIT"] + identifier: biotools:seqkit input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Input fasta/fastq file - pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - fastx: - type: file - description: Output fasta/fastq file - pattern: "*.{fasta.gz,fastq.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - ${prefix}.*: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta.gz,fastq.gz}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/sratools/fasterqdump/meta.yml b/modules/nf-core/sratools/fasterqdump/meta.yml index 6a2151a8..42e2c07c 100644 --- a/modules/nf-core/sratools/fasterqdump/meta.yml +++ b/modules/nf-core/sratools/fasterqdump/meta.yml @@ -1,5 +1,6 @@ name: sratools_fasterqdump -description: Extract sequencing reads in FASTQ format from a given NCBI Sequence Read Archive (SRA). +description: Extract sequencing reads in FASTQ format from a given NCBI Sequence Read + Archive (SRA). keywords: - sequencing - FASTQ @@ -11,42 +12,44 @@ tools: documentation: https://github.com/ncbi/sra-tools/wiki tool_dev_url: https://github.com/ncbi/sra-tools licence: ["Public Domain"] + identifier: "" input: - - meta: - type: map - description: > - Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - - sra: - type: directory - description: Directory containing ETL data for the given SRA. - pattern: "*/*.sra" - - ncbi_settings: - type: file - description: > - An NCBI user settings file. - - pattern: "*.mkfg" - - certificate: - type: file - description: > - Path to a JWT cart file used to access protected dbGAP data on SRA using the sra-toolkit - - pattern: "*.cart" + - - meta: + type: map + description: > + Groovy Map containing sample information e.g. [ id:'test', single_end:false + ] + - sra: + type: directory + description: Directory containing ETL data for the given SRA. + pattern: "*/*.sra" + - - ncbi_settings: + type: file + description: > + An NCBI user settings file. + pattern: "*.mkfg" + - - certificate: + type: file + description: > + Path to a JWT cart file used to access protected dbGAP data on SRA using the + sra-toolkit + pattern: "*.cart" output: - - meta: - type: map - description: > - Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - reads: - type: file - description: Extracted FASTQ file or files if the sequencing reads are paired-end. - pattern: "*.fastq.gz" + - meta: + type: map + description: > + Groovy Map containing sample information e.g. [ id:'test', single_end:false + ] + - "*.fastq.gz": + type: file + description: Extracted FASTQ file or files if the sequencing reads are paired-end. + pattern: "*.fastq.gz" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@Midnighter" maintainers: diff --git a/modules/nf-core/sratools/prefetch/meta.yml b/modules/nf-core/sratools/prefetch/meta.yml index 7ed42d49..3a537bfe 100644 --- a/modules/nf-core/sratools/prefetch/meta.yml +++ b/modules/nf-core/sratools/prefetch/meta.yml @@ -11,45 +11,47 @@ tools: documentation: https://github.com/ncbi/sra-tools/wiki tool_dev_url: https://github.com/ncbi/sra-tools licence: ["Public Domain"] + identifier: "" input: - - meta: - type: map - description: > - Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - - id: - type: string - description: > - A string denoting an SRA id. - - - ncbi_settings: - type: file - description: > - An NCBI user settings file. - - pattern: "*.mkfg" - - certificate: - type: file - description: > - Path to a JWT cart file used to access protected dbGAP data on SRA using the sra-toolkit - - pattern: "*.cart" + - - meta: + type: map + description: > + Groovy Map containing sample information e.g. [ id:'test', single_end:false + ] + - id: + type: string + description: > + A string denoting an SRA id. + - - ncbi_settings: + type: file + description: > + An NCBI user settings file. + pattern: "*.mkfg" + - - certificate: + type: file + description: > + Path to a JWT cart file used to access protected dbGAP data on SRA using the + sra-toolkit + pattern: "*.cart" output: - - meta: - type: map - description: > - Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - sra: - type: directory - description: > - Directory containing the ETL data for the given SRA id. - - pattern: "*/*.sra" + - meta: + type: map + description: > + Groovy Map containing sample information e.g. [ id:'test', single_end:false + ] + pattern: "*/*.sra" + - "id, type: 'dir": + type: map + description: > + Groovy Map containing sample information e.g. [ id:'test', single_end:false + ] + pattern: "*/*.sra" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@Midnighter" maintainers: diff --git a/modules/nf-core/tidk/explore/meta.yml b/modules/nf-core/tidk/explore/meta.yml index 582aaf56..72d15954 100644 --- a/modules/nf-core/tidk/explore/meta.yml +++ b/modules/nf-core/tidk/explore/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "tidk_explore" description: | @@ -10,42 +9,52 @@ keywords: - search tools: - "tidk": - description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + description: tidk is a toolkit to identify and visualise telomeric repeats in + genomes homepage: "https://github.com/tolkit/telomeric-identifier" documentation: "https://github.com/tolkit/telomeric-identifier" tool_dev_url: "https://github.com/tolkit/telomeric-identifier" doi: "10.5281/zenodo.10091385" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: The input fasta file - pattern: "*.{fsa,fa,fasta}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The input fasta file + pattern: "*.{fsa,fa,fasta}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - explore_tsv: - type: file - description: Telomeres and their frequencies in TSV format - pattern: "*.tidk.explore.tsv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.tidk.explore.tsv": + type: file + description: Telomeres and their frequencies in TSV format + pattern: "*.tidk.explore.tsv" - top_sequence: - type: file - description: | - The most frequent telomere sequence if one or more - sequences are identified by the toolkit - pattern: "*.top.sequence.txt" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.top.sequence.txt": + type: file + description: | + The most frequent telomere sequence if one or more + sequences are identified by the toolkit + pattern: "*.top.sequence.txt" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/tidk/plot/meta.yml b/modules/nf-core/tidk/plot/meta.yml index 451195c8..75289b28 100644 --- a/modules/nf-core/tidk/plot/meta.yml +++ b/modules/nf-core/tidk/plot/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "tidk_plot" description: | @@ -11,36 +10,40 @@ keywords: - plot tools: - "tidk": - description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + description: tidk is a toolkit to identify and visualise telomeric repeats in + genomes homepage: "https://github.com/tolkit/telomeric-identifier" documentation: "https://github.com/tolkit/telomeric-identifier" tool_dev_url: "https://github.com/tolkit/telomeric-identifier" doi: "10.5281/zenodo.10091385" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - tsv: - type: file - description: Search results in TSV format from `tidk search` - pattern: "*.tsv" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - tsv: + type: file + description: Search results in TSV format from `tidk search` + pattern: "*.tsv" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - svg: - type: file - description: Telomere search plot - pattern: "*.svg" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.svg": + type: file + description: Telomere search plot + pattern: "*.svg" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/tidk/search/meta.yml b/modules/nf-core/tidk/search/meta.yml index 8ba07350..9a30ff15 100644 --- a/modules/nf-core/tidk/search/meta.yml +++ b/modules/nf-core/tidk/search/meta.yml @@ -1,4 +1,3 @@ ---- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: "tidk_search" description: Searches a genome for a telomere string such as TTAGGG @@ -8,43 +7,53 @@ keywords: - search tools: - "tidk": - description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + description: tidk is a toolkit to identify and visualise telomeric repeats in + genomes homepage: "https://github.com/tolkit/telomeric-identifier" documentation: "https://github.com/tolkit/telomeric-identifier" tool_dev_url: "https://github.com/tolkit/telomeric-identifier" doi: "10.5281/zenodo.10091385" licence: ["MIT"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: The input fasta file - pattern: "*.{fsa,fa,fasta}" - - string: - type: string - description: Search string such as TTAGGG + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The input fasta file + pattern: "*.{fsa,fa,fasta}" + - - string: + type: string + description: Search string such as TTAGGG output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - tsv: - type: file - description: Search results in TSV format - pattern: "*.tsv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.tsv": + type: file + description: Search results in TSV format + pattern: "*.tsv" - bedgraph: - type: file - description: Search results in BEDGRAPH format - pattern: "*.bedgraph" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - "*.bedgraph": + type: file + description: Search results in BEDGRAPH format + pattern: "*.bedgraph" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@GallVp" maintainers: diff --git a/modules/nf-core/umitools/extract/meta.yml b/modules/nf-core/umitools/extract/meta.yml index 7695b271..648ffbd2 100644 --- a/modules/nf-core/umitools/extract/meta.yml +++ b/modules/nf-core/umitools/extract/meta.yml @@ -1,5 +1,6 @@ name: umitools_extract -description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place +description: Extracts UMI barcode from a read and add it to the read name, leaving + any sample barcode in place keywords: - UMI - barcode @@ -8,38 +9,49 @@ keywords: tools: - umi_tools: description: > - UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes + UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random + Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes documentation: https://umi-tools.readthedocs.io/en/latest/ license: "MIT" + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: list - description: | - List of input FASTQ files whose UMIs will be extracted. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: list + description: | + List of input FASTQ files whose UMIs will be extracted. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - reads: - type: file - description: > - Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. - pattern: "*.{fastq.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.fastq.gz": + type: file + description: > + Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. + | For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. + pattern: "*.{fastq.gz}" - log: - type: file - description: Logfile for umi_tools - pattern: "*.{log}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.log": + type: file + description: Logfile for umi_tools + pattern: "*.{log}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@grst" diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml index a9a2110f..290346b3 100644 --- a/modules/nf-core/untar/meta.yml +++ b/modules/nf-core/untar/meta.yml @@ -10,30 +10,33 @@ tools: Extract tar.gz files. documentation: https://www.gnu.org/software/tar/manual/ licence: ["GPL-3.0-or-later"] + identifier: "" input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - archive: - type: file - description: File to be untar - pattern: "*.{tar}.{gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - untar: - type: directory - description: Directory containing contents of archive - pattern: "*/" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - $prefix: + type: directory + description: Directory containing contents of archive + pattern: "*/" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh" diff --git a/subworkflows/gallvp/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap b/subworkflows/gallvp/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap index 660f7e0b..1ec2fb46 100644 --- a/subworkflows/gallvp/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap +++ b/subworkflows/gallvp/gff3_gt_gff3_gff3validator_stat/tests/main.nf.test.snap @@ -18,7 +18,7 @@ ], "3": [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106" + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174" ], "gff3_stats": [ @@ -36,15 +36,15 @@ ], "versions": [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106" + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.3" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-29T16:21:54.482267" + "timestamp": "2024-09-25T11:11:44.756433" }, "sarscov2-genome_gff3-test_fasta-correspondence_fail-out_of_bounds": { "content": [ @@ -53,15 +53,15 @@ ], [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106", + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174", "versions.yml:md5,c89b081a13c68acc5326e43ca9104344" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.3" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-29T16:22:06.684959" + "timestamp": "2024-09-25T11:11:57.27286" }, "sarscov2 - fasta - circular_region - pass": { "content": [ @@ -87,8 +87,8 @@ ], "3": [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174", "versions.yml:md5,80555fe6e28e9564cb534f5478842286", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106", "versions.yml:md5,c89b081a13c68acc5326e43ca9104344" ], "gff3_stats": [ @@ -112,8 +112,8 @@ ], "versions": [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174", "versions.yml:md5,80555fe6e28e9564cb534f5478842286", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106", "versions.yml:md5,c89b081a13c68acc5326e43ca9104344" ] } @@ -122,7 +122,7 @@ "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-09-19T13:53:32.901064" + "timestamp": "2024-09-25T11:12:04.099852" }, "sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail": { "content": [ @@ -131,15 +131,15 @@ ], [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106", + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174", "versions.yml:md5,c89b081a13c68acc5326e43ca9104344" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.3" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-29T16:22:00.684573" + "timestamp": "2024-09-25T11:11:50.869233" }, "sarscov2-genome_gff3-all_pass": { "content": [ @@ -165,8 +165,8 @@ ], "3": [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174", "versions.yml:md5,80555fe6e28e9564cb534f5478842286", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106", "versions.yml:md5,c89b081a13c68acc5326e43ca9104344" ], "gff3_stats": [ @@ -190,16 +190,16 @@ ], "versions": [ "versions.yml:md5,0cb9519e626e5128d8495cf29b7d59ff", + "versions.yml:md5,2f7fe3865b17dd2edde1881a95930174", "versions.yml:md5,80555fe6e28e9564cb534f5478842286", - "versions.yml:md5,8a418ac34d045b0cdac812eb2dc9c106", "versions.yml:md5,c89b081a13c68acc5326e43ca9104344" ] } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.3" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-29T16:21:49.138904" + "timestamp": "2024-09-25T11:11:39.62609" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 2b0dc67a..4d7f09c0 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -3,9 +3,9 @@ // /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NEXTFLOW_PIPELINE { @@ -44,9 +44,9 @@ workflow UTILS_NEXTFLOW_PIPELINE { } /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index b78273ca..805dcc42 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -3,9 +3,9 @@ // /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NFCORE_PIPELINE { @@ -21,9 +21,9 @@ workflow UTILS_NFCORE_PIPELINE { } /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // From 97928c34e8eb12ec9c630da436c7d9916f14f108 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 19:04:15 +1300 Subject: [PATCH 29/62] Now using nf-test for pipeline level testing --- .github/include.yaml | 10 + .github/workflows/ci.yml | 130 ++++---- CHANGELOG.md | 1 + nf-test.config | 12 +- tests/README.md | 17 +- tests/hicparam/assemblysheet.csv | 2 +- tests/hicparam/main.nf.test | 35 ++ tests/hicparam/main.nf.test.snap | 102 ++++++ tests/hicparam/params.json | 6 - tests/hicparam/test_genome.fa.gz | Bin 21 -> 0 bytes tests/invalid/assemblysheet.csv | 4 +- tests/invalid/main.nf.test | 35 ++ tests/invalid/main.nf.test.snap | 54 +++ tests/invalid/params.json | 5 - .../phased2x.mp/assemblysheet.csv.local | 4 - tests/minimal/main.nf.test | 32 ++ tests/minimal/main.nf.test.snap | 53 +++ tests/minimal/params.json | 5 - tests/nextflow.config | 22 ++ tests/noltr/main.nf.test | 55 ++++ tests/noltr/main.nf.test.snap | 89 +++++ tests/noltr/params.json | 6 - tests/stub/main.nf.test | 55 ++++ tests/stub/main.nf.test.snap | 307 ++++++++++++++++++ tests/stub/params.json | 27 -- tests/tiny/main.nf.test | 32 ++ tests/tiny/main.nf.test.snap | 47 +++ tests/tiny/params.json | 5 - 28 files changed, 1023 insertions(+), 129 deletions(-) create mode 100644 .github/include.yaml create mode 100644 tests/hicparam/main.nf.test create mode 100644 tests/hicparam/main.nf.test.snap delete mode 100644 tests/hicparam/params.json delete mode 100644 tests/hicparam/test_genome.fa.gz create mode 100644 tests/invalid/main.nf.test create mode 100644 tests/invalid/main.nf.test.snap delete mode 100644 tests/invalid/params.json delete mode 100644 tests/merqury/phased2x.mp/assemblysheet.csv.local create mode 100644 tests/minimal/main.nf.test create mode 100644 tests/minimal/main.nf.test.snap delete mode 100644 tests/minimal/params.json create mode 100644 tests/nextflow.config create mode 100644 tests/noltr/main.nf.test create mode 100644 tests/noltr/main.nf.test.snap delete mode 100644 tests/noltr/params.json create mode 100644 tests/stub/main.nf.test create mode 100644 tests/stub/main.nf.test.snap delete mode 100644 tests/stub/params.json create mode 100644 tests/tiny/main.nf.test create mode 100644 tests/tiny/main.nf.test.snap delete mode 100644 tests/tiny/params.json diff --git a/.github/include.yaml b/.github/include.yaml new file mode 100644 index 00000000..a3629f4c --- /dev/null +++ b/.github/include.yaml @@ -0,0 +1,10 @@ +".": + - ./.github/workflows/** + - ./nf-test.config + - ./nextflow.config +tests: + - ./assets/* + - ./bin/* + - ./conf/* + - ./main.nf + - ./nextflow_schema.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 583783db..f920d3b9 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,88 +1,106 @@ name: nf-core CI # This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors on: - push: - branches: - - dev pull_request: - release: - types: [published] - workflow_dispatch: env: NXF_ANSI_LOG: false + NFT_VER: "0.9.0" + NFT_WORKDIR: "~" + NFT_DIFF: "pdiff" + NFT_DIFF_ARGS: "--line-numbers --expand-tabs=2" concurrency: group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" cancel-in-progress: true - jobs: - test: - name: Run pipeline with test data - # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'Plant-Food-Research-Open/assemblyqc') }}" + nf-test-changes: + name: Check for changes runs-on: ubuntu-latest - strategy: - matrix: - NXF_VER: - - "24.04.2" - TEST_PARAMS: - - minimal - - invalid - - stub - - noltr - - hicparam - include: - - OPTION_STUB: "" - - OPTION_STUB: "-stub" - TEST_PARAMS: stub - - OPTION_STUB: "-stub" - TEST_PARAMS: hicparam + outputs: + nf_test_files: ${{ steps.list.outputs.components }} steps: - - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - - name: Install Nextflow - uses: nf-core/setup-nextflow@v2 + - uses: actions/checkout@v4.2.1 with: - version: ${{ matrix.NXF_VER }} + fetch-depth: 0 - - name: Disk space cleanup - uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: List nf-test files + id: list + uses: adamrtalbot/detect-nf-test-changes@v0.0.4 + with: + head: ${{ github.sha }} + base: origin/${{ github.base_ref }} + include: .github/include.yaml - - name: Run pipeline with test data + - name: print list of nf-test files run: | - nextflow run \ - ${GITHUB_WORKSPACE} \ - -profile test,docker \ - -params-file ./tests/${{ matrix.TEST_PARAMS }}/params.json \ - ${{ matrix.OPTION_STUB }} \ - --outdir ./results - - nf-test: - name: Run nf-tests - # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'Plant-Food-Research-Open/assemblyqc') }}" + echo ${{ steps.list.outputs.components }} + + test: + name: ${{ matrix.nf_test_files }} ${{ matrix.profile }} NF-${{ matrix.NXF_VER }} + needs: [nf-test-changes] + if: needs.nf-test-changes.outputs.nf_test_files != '[]' runs-on: ubuntu-latest strategy: + fail-fast: false matrix: NXF_VER: - "24.04.2" + + nf_test_files: ["${{ fromJson(needs.nf-test-changes.outputs.nf_test_files) }}"] + profile: + - "docker" + steps: - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + uses: actions/checkout@v4.2.1 - name: Install Nextflow uses: nf-core/setup-nextflow@v2 with: - version: ${{ matrix.NXF_VER }} + version: "${{ matrix.NXF_VER }}" + + - uses: actions/setup-python@v5.2.0 + with: + python-version: "3.11" + architecture: "x64" + + - name: Install pdiff to see diff between nf-test snapshots + run: | + python -m pip install --upgrade pip + pip install pdiff + + - uses: nf-core/setup-nf-test@v1.1.2 + + - name: Run nf-test + run: | + nf-test test --verbose ${{ matrix.nf_test_files }} --profile "+${{ matrix.profile }}" --tap=test.tap + + - uses: pcolby/tap-summary@v1.1.1 + with: + path: >- + test.tap - - name: Install nf-test - uses: nf-core/setup-nf-test@v1.1.2 + - name: Output log on failure + if: failure() + run: | + sudo apt install bat > /dev/null + batcat --decorations=always --color=always ${{ github.workspace }}/.nf-test/tests/*/meta/nextflow.log + + confirm-pass: + runs-on: ubuntu-latest + needs: [test] + if: always() + steps: + - name: All tests ok + if: ${{ !contains(needs.*.result, 'failure') }} + run: exit 0 + - name: One or more tests failed + if: ${{ contains(needs.*.result, 'failure') }} + run: exit 1 - - name: Run nf-tests + - name: debug-print + if: always() run: | - nf-test \ - test \ - --verbose \ - tests + echo "toJSON(needs) = ${{ toJSON(needs) }}" + echo "toJSON(needs.*.result) = ${{ toJSON(needs.*.result) }}" diff --git a/CHANGELOG.md b/CHANGELOG.md index 99704fe9..749d7926 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 1. Added Gfastats [#126](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/126) 2. Updated nf-core/template to 3.0.1 [#149](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/149) 3. Updated `samtools faidx` to 1.21 +4. Now using nf-test for pipeline level testing [#153](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/153) ### `Fixed` diff --git a/nf-test.config b/nf-test.config index 9d4198ff..042da10d 100644 --- a/nf-test.config +++ b/nf-test.config @@ -1,8 +1,10 @@ config { + testsDir "." + workDir System.getenv("NFT_WORKDIR") ?: ".nf-test" + configFile "tests/nextflow.config" - testsDir "tests" - workDir ".nf-test" - configFile "nextflow.config" - profile "" - + plugins { + load "nft-bam@0.4.0" + load "nft-utils@0.0.3" + } } diff --git a/tests/README.md b/tests/README.md index 257defe3..6e935db0 100644 --- a/tests/README.md +++ b/tests/README.md @@ -14,14 +14,17 @@ Or using [singularity](https://docs.sylabs.io/guides/3.0/user-guide/installation nextflow run plant-food-research-open/assemblyqc -r main -profile singularity,test --outdir results ``` -## Local Testing +## nf-test and Continuous Integration (CI) -The test sets included in this directory can be executed by first downloading the pipeline from GitHub and then executing the following command: +The GitHub [CI action](../.github/workflows/ci.yml) included with the pipeline continuously tests the pipeline using [nf-test](https://www.nf-test.com). -```bash -./main.nf -profile docker -params-file tests/minimal/params.json --outdir results -``` +## Testing Merqury Datasets -## Continuous Integration (CI) +Three Merqury datasets are included here which can be tested by pointing to one of the parameters file. -The GitHub [CI action](../.github/workflows/ci.yml) included with the pipeline continuously tests the pipeline with the various test sets listed in this directory. +```bash +./main.nf \ + -profile \ + -params-file tests/merqury//params.json \ + --outdir results +``` diff --git a/tests/hicparam/assemblysheet.csv b/tests/hicparam/assemblysheet.csv index 19e256c0..222ecb0b 100644 --- a/tests/hicparam/assemblysheet.csv +++ b/tests/hicparam/assemblysheet.csv @@ -1,2 +1,2 @@ tag,fasta -test,tests/hicparam/test_genome.fa.gz +test,https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/genome/genome.fasta diff --git a/tests/hicparam/main.nf.test b/tests/hicparam/main.nf.test new file mode 100644 index 00000000..064dc0c6 --- /dev/null +++ b/tests/hicparam/main.nf.test @@ -0,0 +1,35 @@ +nextflow_pipeline { + + name "Test with hic param" + script "main.nf" + + test("hic param - stub") { + + options '-stub' + + when { + params { + input = "$baseDir/tests/hicparam/assemblysheet.csv" + hic = "$baseDir/tests/hicparam/hic/Dummy_hic_{1,2}.merged.fq.gz" + outdir = "$outputDir" + } + } + + then { + def stable_path = getAllFilesFromDir(params.outdir, false, ['pipeline_info/*.{html,json,txt,yml}', 'report.{html,json}'], null, ['**']) + + assertAll( + { assert workflow.success}, + { assert snapshot( + [ + 'successful tasks': workflow.trace.succeeded().size(), + 'versions': removeNextflowVersion("$outputDir/pipeline_info/software_versions.yml"), + 'stable paths': stable_path + ] + ).match() } + ) + } + + } + +} diff --git a/tests/hicparam/main.nf.test.snap b/tests/hicparam/main.nf.test.snap new file mode 100644 index 00000000..6ca72d89 --- /dev/null +++ b/tests/hicparam/main.nf.test.snap @@ -0,0 +1,102 @@ +{ + "hic param - stub": { + "content": [ + { + "successful tasks": 21, + "versions": { + "AGP2ASSEMBLY": { + "juicebox_scripts": "0.1.0" + }, + "ASSEMBLATHON_STATS": { + "assemblathon_stats": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + }, + "ASSEMBLY2BEDPE": { + "python": "3.11.3", + "pandas": "2.1.1" + }, + "BWA_INDEX": { + "bwa": "0.7.18-r1243-dirty" + }, + "BWA_MEM": { + "bwa": "0.7.18-r1243-dirty", + "samtools": 1.2 + }, + "FASTAVALIDATOR": { + "py_fasta_validator": 0.6 + }, + "FASTP": { + "fastp": "0.23.4" + }, + "FASTQC_RAW": { + "fastqc": "0.12.1" + }, + "FASTQC_TRIM": { + "fastqc": "0.12.1" + }, + "HIC2HTML": { + "python": "3.11.3" + }, + "HICQC": { + "hic_qc.py": "0+untagged.261.g6881c33" + }, + "JUICER_SORT": { + "sort": 8.3 + }, + "MAKEAGPFROMFASTA": { + "juicebox_scripts": "0.1.0" + }, + "MATLOCK_BAM2_JUICER": { + "matlock": 20181227 + }, + "RUNASSEMBLYVISUALIZER": { + "run-assembly-visualizer.sh": "18 July 2016" + }, + "SAMBLASTER": { + "samblaster": "0.1.26", + "samtools": "1.19.2" + }, + "SAMTOOLS_FAIDX": { + "samtools": 1.21 + }, + "SEQKIT_RMDUP": { + "seqkit": "v2.8.0" + }, + "SEQKIT_SORT": { + "seqkit": "v2.8.0" + }, + "TAG_ASSEMBLY": { + "pigz": "2.3.4" + }, + "Workflow": { + "plant-food-research-open/assemblyqc": "v2.2.0+dev" + } + }, + "stable paths": [ + "test_stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "test.agp.assembly:md5,d41d8cd98f00b204e9800998ecf8427e", + "test.assembly.bedpe:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.paired.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "Dummy_hic_1.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "Dummy_hic_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "Dummy_hic_2.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "Dummy_hic_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "Dummy_hic.html:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.zip:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.html:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.zip:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.on.test.pdf:md5,d41d8cd98f00b204e9800998ecf8427e", + "test.hic:md5,d41d8cd98f00b204e9800998ecf8427e", + "test.html:md5,bbd8f07f11522eb75bb9429e86e95713" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-10T16:11:00.660108" + } +} \ No newline at end of file diff --git a/tests/hicparam/params.json b/tests/hicparam/params.json deleted file mode 100644 index c99d6c4b..00000000 --- a/tests/hicparam/params.json +++ /dev/null @@ -1,6 +0,0 @@ -{ - "config_profile_name": "Test to verify hic param validation", - "config_profile_description": "Test to verify hic param validation", - "input": "tests/hicparam/assemblysheet.csv", - "hic": "tests/hicparam/hic/Dummy_hic_{1,2}.merged.fq.gz" -} diff --git a/tests/hicparam/test_genome.fa.gz b/tests/hicparam/test_genome.fa.gz deleted file mode 100644 index 1a720c1f68ed6c8c0d0c598ecd22b96aff9a2bd8..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 21 ccmb2|=3q#2d!5F>{FsShGTU_{Mg|5306`K3Hvj+t diff --git a/tests/invalid/assemblysheet.csv b/tests/invalid/assemblysheet.csv index 93e81420..bfee9e8d 100644 --- a/tests/invalid/assemblysheet.csv +++ b/tests/invalid/assemblysheet.csv @@ -1,5 +1,5 @@ tag,fasta,gff3 FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz TT_2021a,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz -MISC,tests/invalid/invalid.fsa.gz -DUPSEQ,tests/invalid/dupseq.fsa.gz +MISC,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/invalid/invalid.fsa.gz +DUPSEQ,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/invalid/dupseq.fsa.gz diff --git a/tests/invalid/main.nf.test b/tests/invalid/main.nf.test new file mode 100644 index 00000000..93cb3310 --- /dev/null +++ b/tests/invalid/main.nf.test @@ -0,0 +1,35 @@ +nextflow_pipeline { + + name "Test with invalid input files" + script "main.nf" + + test("invalid") { + + when { + params { + input = "$baseDir/tests/invalid/assemblysheet.csv" + outdir = "$outputDir" + } + } + + then { + def stable_path = getAllFilesFromDir(params.outdir, false, ['pipeline_info/*.{html,json,txt,yml}', 'report.{html,json}'], null, ['**']) + + assertAll( + { assert workflow.success}, + { assert 'WARN: GFF3 validation failed for TT_2021a' in workflow.stdout }, + { assert 'WARN: FASTA validation failed for MISC' in workflow.stdout }, + { assert 'WARN: FASTA validation failed for DUPSEQ due to presence of duplicate sequences' in workflow.stdout }, + { assert snapshot( + [ + 'successful tasks': workflow.trace.succeeded().size(), + 'versions': removeNextflowVersion("$outputDir/pipeline_info/software_versions.yml"), + 'stable paths': stable_path + ] + ).match() } + ) + } + + } + +} diff --git a/tests/invalid/main.nf.test.snap b/tests/invalid/main.nf.test.snap new file mode 100644 index 00000000..bdd1665d --- /dev/null +++ b/tests/invalid/main.nf.test.snap @@ -0,0 +1,54 @@ +{ + "invalid": { + "content": [ + { + "successful tasks": 25, + "versions": { + "ASSEMBLATHON_STATS": { + "assemblathon_stats": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + }, + "FASTAVALIDATOR": { + "py_fasta_validator": 0.6 + }, + "GT_GFF3": { + "genometools": "1.6.5" + }, + "GT_GFF3VALIDATOR": { + "genometools": "1.6.5" + }, + "GT_STAT": { + "genometools": "1.6.5" + }, + "GUNZIP_FASTA": { + "gunzip": 1.1 + }, + "GUNZIP_GFF3": { + "gunzip": 1.1 + }, + "SAMTOOLS_FAIDX": { + "samtools": 1.21 + }, + "SEQKIT_RMDUP": { + "seqkit": "v2.8.0" + }, + "TAG_ASSEMBLY": { + "pigz": "2.3.4" + }, + "Workflow": { + "plant-food-research-open/assemblyqc": "v2.2.0+dev" + } + }, + "stable paths": [ + "FI1_stats.csv:md5,8d1274e52117e39b413ff71a76c40331", + "TT_2021a_stats.csv:md5,55fa0923a6d47fdd19201486848b48fe", + "FI1.gt.stat.yml:md5,2fc1b9c84af0c2323d78a9ac623a3022" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-10T15:59:12.150838" + } +} \ No newline at end of file diff --git a/tests/invalid/params.json b/tests/invalid/params.json deleted file mode 100644 index eb2dab01..00000000 --- a/tests/invalid/params.json +++ /dev/null @@ -1,5 +0,0 @@ -{ - "config_profile_name": "Invalid profile", - "config_profile_description": "Profile to test invalid files", - "input": "tests/invalid/assemblysheet.csv" -} diff --git a/tests/merqury/phased2x.mp/assemblysheet.csv.local b/tests/merqury/phased2x.mp/assemblysheet.csv.local deleted file mode 100644 index f90aec6f..00000000 --- a/tests/merqury/phased2x.mp/assemblysheet.csv.local +++ /dev/null @@ -1,4 +0,0 @@ -tag,fasta,reads_1,reads_2,maternal_reads_1,paternal_reads_1 -COL,https://gembox.cbcb.umd.edu/triobinning/athal_COL.fasta,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_2.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_CVI.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_COL.fastq.gz -CVI,https://gembox.cbcb.umd.edu/triobinning/athal_CVI.fasta,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR3703081_2.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_CVI.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/athal_COL.fastq.gz -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR8238189_1.fastq.gz,/Users/hrauxr/Projects/test-data/assemblyqc/merqury.fk/phased2x.mp/SRR8238189_2.fastq.gz diff --git a/tests/minimal/main.nf.test b/tests/minimal/main.nf.test new file mode 100644 index 00000000..b4dae9be --- /dev/null +++ b/tests/minimal/main.nf.test @@ -0,0 +1,32 @@ +nextflow_pipeline { + + name "Test with minimal input" + script "main.nf" + + test("minimal") { + + when { + params { + input = "$baseDir/assets/assemblysheetv2.csv" + outdir = "$outputDir" + } + } + + then { + def stable_path = getAllFilesFromDir(params.outdir, false, ['pipeline_info/*.{html,json,txt,yml}', 'report.{html,json}'], null, ['**']) + + assertAll( + { assert workflow.success}, + { assert snapshot( + [ + 'successful tasks': workflow.trace.succeeded().size(), + 'versions': removeNextflowVersion("$outputDir/pipeline_info/software_versions.yml"), + 'stable paths': stable_path + ] + ).match() } + ) + } + + } + +} diff --git a/tests/minimal/main.nf.test.snap b/tests/minimal/main.nf.test.snap new file mode 100644 index 00000000..7538396f --- /dev/null +++ b/tests/minimal/main.nf.test.snap @@ -0,0 +1,53 @@ +{ + "minimal": { + "content": [ + { + "successful tasks": 11, + "versions": { + "ASSEMBLATHON_STATS": { + "assemblathon_stats": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + }, + "FASTAVALIDATOR": { + "py_fasta_validator": 0.6 + }, + "GT_GFF3": { + "genometools": "1.6.5" + }, + "GT_GFF3VALIDATOR": { + "genometools": "1.6.5" + }, + "GT_STAT": { + "genometools": "1.6.5" + }, + "GUNZIP_FASTA": { + "gunzip": 1.1 + }, + "GUNZIP_GFF3": { + "gunzip": 1.1 + }, + "SAMTOOLS_FAIDX": { + "samtools": 1.21 + }, + "SEQKIT_RMDUP": { + "seqkit": "v2.8.0" + }, + "TAG_ASSEMBLY": { + "pigz": "2.3.4" + }, + "Workflow": { + "plant-food-research-open/assemblyqc": "v2.2.0+dev" + } + }, + "stable paths": [ + "FI1_stats.csv:md5,8d1274e52117e39b413ff71a76c40331", + "FI1.gt.stat.yml:md5,2fc1b9c84af0c2323d78a9ac623a3022" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-10T14:37:54.336611" + } +} diff --git a/tests/minimal/params.json b/tests/minimal/params.json deleted file mode 100644 index 0b8c5117..00000000 --- a/tests/minimal/params.json +++ /dev/null @@ -1,5 +0,0 @@ -{ - "config_profile_name": "Test profile", - "config_profile_description": "Minimal test dataset to check pipeline function", - "input": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheetv2.csv" -} diff --git a/tests/nextflow.config b/tests/nextflow.config new file mode 100644 index 00000000..1d5de542 --- /dev/null +++ b/tests/nextflow.config @@ -0,0 +1,22 @@ +/* +======================================================================================== + Nextflow config file for running tests +======================================================================================== +*/ + +params { + modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' +} + +timeline { enabled = false } +report { enabled = false } +trace { enabled = false } +dag { enabled = false } + +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} diff --git a/tests/noltr/main.nf.test b/tests/noltr/main.nf.test new file mode 100644 index 00000000..98f9e1ce --- /dev/null +++ b/tests/noltr/main.nf.test @@ -0,0 +1,55 @@ +nextflow_pipeline { + + name "Test with a genome which does not have LTRs" + script "main.nf" + + test("noltr") { + + when { + params { + input = "$baseDir/tests/noltr/assemblysheet.csv" + lai_skip = false + outdir = "$outputDir" + } + } + + then { + def stable_path = getAllFilesFromDir( + params.outdir, + false, + [ + 'pipeline_info/*.{html,json,txt,yml}', + 'report.{html,json}', + 'lai/*.LAI.log', + 'lai/*.restored.ids.gff3' + ], + null, + ['**'] + ) + + def stable_name = getAllFilesFromDir( + params.outdir, + true, + [ + 'pipeline_info/*.{html,json,txt,yml}' + ], + null, + ['**'] + ) + + assertAll( + { assert workflow.success}, + { assert snapshot( + [ + 'successful tasks': workflow.trace.succeeded().size(), + 'versions': removeNextflowVersion("$outputDir/pipeline_info/software_versions.yml"), + 'stable paths': stable_path, + 'stable names': getRelativePath(stable_name, outputDir), + ] + ).match() } + ) + } + + } + +} diff --git a/tests/noltr/main.nf.test.snap b/tests/noltr/main.nf.test.snap new file mode 100644 index 00000000..c336d8a5 --- /dev/null +++ b/tests/noltr/main.nf.test.snap @@ -0,0 +1,89 @@ +{ + "noltr": { + "content": [ + { + "successful tasks": 26, + "versions": { + "ASSEMBLATHON_STATS": { + "assemblathon_stats": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + }, + "CAT_CAT": { + "pigz": "2.3.4" + }, + "CUSTOM_RESTOREGFFIDS": { + "python": "3.10.2" + }, + "CUSTOM_SHORTENFASTAIDS": { + "python": "3.8.13", + "biopython": 1.75 + }, + "FASTAVALIDATOR": { + "py_fasta_validator": 0.6 + }, + "GUNZIP_FASTA": { + "gunzip": 1.1 + }, + "LTRFINDER": { + "LTR_FINDER_parallel": "v1.1", + "ltr_finder": "v1.07" + }, + "LTRHARVEST": { + "LTR_HARVEST_parallel": "v1.1", + "genometools": "1.6.5" + }, + "LTRRETRIEVER_LAI": { + "lai": "beta3.2" + }, + "LTRRETRIEVER_LTRRETRIEVER": { + "LTR_retriever": "v2.9.9" + }, + "SAMTOOLS_FAIDX": { + "samtools": 1.21 + }, + "SEQKIT_RMDUP": { + "seqkit": "v2.8.0" + }, + "TAG_ASSEMBLY": { + "pigz": "2.3.4" + }, + "UNMASK_IF_ANY": { + "seqkit": "v2.8.0" + }, + "Workflow": { + "plant-food-research-open/assemblyqc": "v2.2.0+dev" + } + }, + "stable paths": [ + "FI1_stats.csv:md5,8d1274e52117e39b413ff71a76c40331", + "sarscov2_stats.csv:md5,9ec4147b73cae550a38337520fc028dd", + "FI1.LAI.out:md5,c958c2f4187e0ae42060d304556e0e6a", + "FI1.LTRlib.fa:md5,e7cc7ee7269dd5a09773cfbedba0de06", + "FI1.short.ids.tsv:md5,948c19c1ccd05463a1f5d23f0615b169", + "sarscov2.short.ids.tsv:md5,d7a2af88e8549586e5616bff6a88bd71" + ], + "stable names": [ + "assemblathon_stats", + "assemblathon_stats/FI1_stats.csv", + "assemblathon_stats/sarscov2_stats.csv", + "lai", + "lai/FI1.LAI.log", + "lai/FI1.LAI.out", + "lai/FI1.LTRlib.fa", + "lai/FI1.restored.ids.gff3", + "lai/FI1.short.ids.tsv", + "lai/sarscov2.short.ids.tsv", + "pipeline_info", + "report.html", + "report.json", + "synteny", + "synteny/plotsr" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-10T15:40:52.594249" + } +} \ No newline at end of file diff --git a/tests/noltr/params.json b/tests/noltr/params.json deleted file mode 100644 index e52470db..00000000 --- a/tests/noltr/params.json +++ /dev/null @@ -1,6 +0,0 @@ -{ - "config_profile_name": "No LTRs assembly profile", - "config_profile_description": "Profile to test an assembly without LTRs", - "input": "tests/noltr/assemblysheet.csv", - "lai_skip": false -} diff --git a/tests/stub/main.nf.test b/tests/stub/main.nf.test new file mode 100644 index 00000000..528ec8b5 --- /dev/null +++ b/tests/stub/main.nf.test @@ -0,0 +1,55 @@ +nextflow_pipeline { + + name "Test the entire pipeline in stub mode" + script "main.nf" + + test("full - stub") { + options '-stub' + + when { + params { + input = "$baseDir/assets/assemblysheetv2.csv" + gfastats_skip = false + ncbi_fcs_adaptor_skip = false + ncbi_fcs_adaptor_empire = "euk" + ncbi_fcs_gx_skip = false + ncbi_fcs_gx_tax_id = 12 + ncbi_fcs_gx_db_path = "$baseDir/tests/stub/gxdb/test" + busco_skip = false + busco_mode = "genome" + busco_lineage_datasets = "fungi_odb10 hypocreales_odb10" + tidk_skip = false + tidk_repeat_seq = "TTTGGG" + lai_skip = false + kraken2_skip = false + kraken2_db_path = "$baseDir/tests/stub/kraken2/k2_minusb_20231009.tar.gz" + hic = "$baseDir/tests/stub/hic/Dummy_hic.R{1,2}.fq.gz" + hic_skip_fastp = true + hic_skip_fastqc = false + synteny_skip = false + synteny_mummer_skip = false + synteny_plotsr_skip = false + synteny_xref_assemblies = "$baseDir/assets/xrefsheet.csv" + merqury_skip = false + outdir = "$outputDir" + } + } + + then { + def stable_path = getAllFilesFromDir(params.outdir, false, ['pipeline_info/*.{html,json,txt,yml}', 'report.{html,json}'], null, ['**']) + + assertAll( + { assert workflow.success}, + { assert snapshot( + [ + 'successful tasks': workflow.trace.succeeded().size(), + 'versions': removeNextflowVersion("$outputDir/pipeline_info/software_versions.yml"), + 'stable paths': stable_path + ] + ).match() } + ) + } + + } + +} diff --git a/tests/stub/main.nf.test.snap b/tests/stub/main.nf.test.snap new file mode 100644 index 00000000..257765b7 --- /dev/null +++ b/tests/stub/main.nf.test.snap @@ -0,0 +1,307 @@ +{ + "full - stub": { + "content": [ + { + "successful tasks": 91, + "versions": { + "AGP2ASSEMBLY": { + "juicebox_scripts": "0.1.0" + }, + "ASSEMBLATHON_STATS": { + "assemblathon_stats": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + }, + "ASSEMBLY2BEDPE": { + "python": "3.11.3", + "pandas": "2.1.1" + }, + "BUNDLELINKS": { + "python": "3.11.3" + }, + "BUSCO_ANNOTATION": { + "busco": "5.7.1" + }, + "BUSCO_ASSEMBLY": { + "busco": "5.7.1" + }, + "BWA_INDEX": { + "bwa": "0.7.18-r1243-dirty" + }, + "BWA_MEM": { + "bwa": "0.7.18-r1243-dirty", + "samtools": 1.2 + }, + "CAT_CAT": { + "pigz": "2.3.4" + }, + "CIRCOS": { + "circos": "v0.69-8", + "perl": 5.032001 + }, + "COLOURBUNDLELINKS": { + "python": "3.11.3", + "perl": "5.32.1" + }, + "CUSTOM_RELABELFASTA": { + "python": "3.8.13", + "biopython": 1.75 + }, + "CUSTOM_SHORTENFASTAIDS": { + "python": "3.8.13", + "biopython": 1.75 + }, + "CUSTOM_SRATOOLSNCBISETTINGS": { + "sratools": "3.0.8" + }, + "DNADIFF": { + "dnadiff": 1.3 + }, + "EXTRACT_PROTEINS": { + "gffread": "0.12.7" + }, + "FASTAVALIDATOR": { + "py_fasta_validator": 0.6 + }, + "FASTQC_RAW": { + "fastqc": "0.12.1" + }, + "FCS_FCSADAPTOR": { + "FCS-adaptor": "0.5.0" + }, + "FILTERSORTFASTA": { + "samtools": "1.16.1" + }, + "FILTER_BY_LENGTH": { + "seqkit": "v2.8.0" + }, + "GENERATEKARYOTYPE": { + "awk": "1.3.4 20200120", + "grep": "(GNU grep) 3.4", + "sed": "(GNU sed) 4.7" + }, + "GETFASTALENGTH": { + "samtools": "1.16.1" + }, + "GFASTATS": { + "gfastats": "1.3.6" + }, + "GT_GFF3": { + "genometools": "1.6.5" + }, + "GT_GFF3VALIDATOR": { + "genometools": "1.6.5" + }, + "GT_STAT": { + "genometools": "1.6.5" + }, + "GUNZIP_FASTA": { + "gunzip": 1.1 + }, + "GUNZIP_GFF3": { + "gunzip": 1.1 + }, + "HIC2HTML": { + "python": "3.11.3" + }, + "HICQC": { + "hic_qc.py": "0+untagged.261.g6881c33" + }, + "JUICER_SORT": { + "sort": 8.3 + }, + "KRAKEN2": { + "kraken2": "2.1.2" + }, + "KRAKEN2_KRONA_PLOT": { + "KronaTools": "2.7.1" + }, + "LINEARSYNTENY": { + "python": "3.11.3", + "pandas": "2.1.1", + "plotly": "5.20.0" + }, + "LTRFINDER": { + "LTR_FINDER_parallel": "v1.1", + "ltr_finder": "v1.07" + }, + "LTRHARVEST": { + "LTR_HARVEST_parallel": "v1.1", + "genometools": "1.6.5" + }, + "LTRRETRIEVER_LAI": { + "lai": "beta3.2" + }, + "LTRRETRIEVER_LTRRETRIEVER": { + "LTR_retriever": "v2.9.9" + }, + "MAKEAGPFROMFASTA": { + "juicebox_scripts": "0.1.0" + }, + "MATLOCK_BAM2_JUICER": { + "matlock": 20181227 + }, + "MERQURY_MERQURY": { + "merqury": 1.3 + }, + "MERYL_COUNT": { + "meryl": "1.4.1" + }, + "MERYL_UNIONSUM": { + "meryl": "1.4.1" + }, + "MINIMAP2_ALIGN": { + "minimap2": "2.28-r1209" + }, + "MUMMER": { + "nucmer": "4.0.0rc1" + }, + "NCBI_FCS_GX_KRONA_PLOT": { + "KronaTools": "2.7.1" + }, + "NCBI_FCS_GX_SCREEN_SAMPLES": { + "fcs_gx": "0.5.4" + }, + "NCBI_FCS_GX_SETUP_SAMPLE": { + "ubuntu": "20.04.6l" + }, + "PLOTSR": { + "plotsr": "1.1.1" + }, + "RELABELBUNDLELINKS": { + "python": "3.11.3" + }, + "RELABELFASTALENGTH": { + "python": "3.11.3" + }, + "RUNASSEMBLYVISUALIZER": { + "run-assembly-visualizer.sh": "18 July 2016" + }, + "SAMBLASTER": { + "samblaster": "0.1.26", + "samtools": "1.19.2" + }, + "SAMTOOLS_FAIDX": { + "samtools": 1.21 + }, + "SEQKIT_RMDUP": { + "seqkit": "v2.8.0" + }, + "SEQKIT_SORT": { + "seqkit": "v2.8.0" + }, + "SORT_BY_LENGTH": { + "seqkit": "v2.8.0" + }, + "SPLITBUNDLEFILE": { + "awk": "1.3.4 20200120" + }, + "SRATOOLS_FASTERQDUMP": { + "sratools": "3.0.8", + "pigz": 2.6 + }, + "SRATOOLS_PREFETCH": { + "sratools": "3.1.0", + "curl": "8.5.0" + }, + "SYRI": { + "syri": "1.7.0" + }, + "TAG_ASSEMBLY": { + "pigz": "2.3.4" + }, + "TIDK_EXPLORE": { + "tidk": "0.2.41" + }, + "TIDK_PLOT_APOSTERIORI": { + "tidk": "0.2.41" + }, + "TIDK_PLOT_APRIORI": { + "tidk": "0.2.41" + }, + "TIDK_SEARCH_APOSTERIORI": { + "tidk": "0.2.41" + }, + "TIDK_SEARCH_APRIORI": { + "tidk": "0.2.41" + }, + "UNMASK_IF_ANY": { + "seqkit": "v2.8.0" + }, + "UNTAR": { + "untar": 1.34 + }, + "Workflow": { + "plant-food-research-open/assemblyqc": "v2.2.0+dev" + } + }, + "stable paths": [ + "FI1_stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.gt.stat.yml:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.assembly_summary:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.hic:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.html:md5,6bd2e97a3a2199609c2f2a5d4d314b1e", + "FI1.agp.assembly:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.assembly.bedpe:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.R.html:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.R.zip:md5,d41d8cd98f00b204e9800998ecf8427e", + "Dummy_hic.R.on.FI1.pdf:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.kraken2.cut:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.kraken2.krona.cut:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.kraken2.krona.html:md5,a629f1aab2e8dce3e921119498d669fd", + "FI1.kraken2.report:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.LTRlib.fa:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.short.ids.tsv:md5,fcf920d9a7b57a1e3c29a9e88673330f", + "FI1.FI1.qv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.completeness.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.dist_only.hist:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.hist.ploidy:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.qv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-asm.fl.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-asm.hist:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-asm.ln.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-asm.st.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-cn.fl.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-cn.hist:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-cn.ln.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.spectra-cn.st.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1_only.bed:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1_only.wig:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.fcs_adaptor_report.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.fcs.gx.krona.cut:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.fcs.gx.krona.html:md5,4e24c1bdb5d3e2666e647390a9ececce", + "FI1.fcs_gx_report.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.inter.tax.rpt.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.taxonomy.rpt:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.on.JAD.all.html:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.on.JAD.all.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.on.JAD.all.svg:md5,d41d8cd98f00b204e9800998ecf8427e", + "bundled.links.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "circos.conf:md5,d41d8cd98f00b204e9800998ecf8427e", + "karyotype.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.on.TT_2021a.all.html:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.on.TT_2021a.all.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.on.TT_2021a.all.svg:md5,d41d8cd98f00b204e9800998ecf8427e", + "bundled.links.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "circos.conf:md5,d41d8cd98f00b204e9800998ecf8427e", + "karyotype.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.plotsr.csv:md5,d2e982f8f4fb8e32dc2f0ae74e688aef", + "JAD.plotsr.csv:md5,1c9f46f53f33361b80e126cc5d410c7d", + "TT_2021a.plotsr.csv:md5,dba0dd1f7c25e345052363030deace6b", + "plotsr.png:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.aposteriori.svg:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.aposteriori.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.apriori.svg:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.apriori.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.tidk.explore.tsv:md5,d41d8cd98f00b204e9800998ecf8427e", + "FI1.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-10T14:56:44.730644" + } +} \ No newline at end of file diff --git a/tests/stub/params.json b/tests/stub/params.json deleted file mode 100644 index 85bdf0ce..00000000 --- a/tests/stub/params.json +++ /dev/null @@ -1,27 +0,0 @@ -{ - "config_profile_name": "Full stub test", - "config_profile_description": "Full test of the pipeline in stub mode", - "input": "assets/assemblysheetv2.csv", - "gfastats_skip": false, - "ncbi_fcs_adaptor_skip": false, - "ncbi_fcs_adaptor_empire": "euk", - "ncbi_fcs_gx_skip": false, - "ncbi_fcs_gx_tax_id": 12, - "ncbi_fcs_gx_db_path": "tests/stub/gxdb/test", - "busco_skip": false, - "busco_mode": "genome", - "busco_lineage_datasets": "fungi_odb10 hypocreales_odb10", - "tidk_skip": false, - "tidk_repeat_seq": "TTTGGG", - "lai_skip": false, - "kraken2_skip": false, - "kraken2_db_path": "tests/stub/kraken2/k2_minusb_20231009.tar.gz", - "hic": "tests/stub/hic/Dummy_hic.R{1,2}.fq.gz", - "hic_skip_fastp": true, - "hic_skip_fastqc": false, - "synteny_skip": false, - "synteny_mummer_skip": false, - "synteny_plotsr_skip": false, - "synteny_xref_assemblies": "assets/xrefsheet.csv", - "merqury_skip": false -} diff --git a/tests/tiny/main.nf.test b/tests/tiny/main.nf.test new file mode 100644 index 00000000..c3254e9f --- /dev/null +++ b/tests/tiny/main.nf.test @@ -0,0 +1,32 @@ +nextflow_pipeline { + + name "Test with a tiny input genome" + script "main.nf" + + test("tiny") { + + when { + params { + input = "$baseDir/tests/tiny/assemblysheet.csv" + outdir = "$outputDir" + } + } + + then { + def stable_path = getAllFilesFromDir(params.outdir, false, ['pipeline_info/*.{html,json,txt,yml}', 'report.{html,json}'], null, ['**']) + + assertAll( + { assert workflow.success}, + { assert snapshot( + [ + 'successful tasks': workflow.trace.succeeded().size(), + 'versions': removeNextflowVersion("$outputDir/pipeline_info/software_versions.yml"), + 'stable paths': stable_path + ] + ).match() } + ) + } + + } + +} diff --git a/tests/tiny/main.nf.test.snap b/tests/tiny/main.nf.test.snap new file mode 100644 index 00000000..c3a241a3 --- /dev/null +++ b/tests/tiny/main.nf.test.snap @@ -0,0 +1,47 @@ +{ + "tiny": { + "content": [ + { + "successful tasks": 9, + "versions": { + "ASSEMBLATHON_STATS": { + "assemblathon_stats": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + }, + "FASTAVALIDATOR": { + "py_fasta_validator": 0.6 + }, + "GT_GFF3": { + "genometools": "1.6.5" + }, + "GT_GFF3VALIDATOR": { + "genometools": "1.6.5" + }, + "GT_STAT": { + "genometools": "1.6.5" + }, + "SAMTOOLS_FAIDX": { + "samtools": 1.21 + }, + "SEQKIT_RMDUP": { + "seqkit": "v2.8.0" + }, + "TAG_ASSEMBLY": { + "pigz": "2.3.4" + }, + "Workflow": { + "plant-food-research-open/assemblyqc": "v2.2.0+dev" + } + }, + "stable paths": [ + "sarscov2_stats.csv:md5,9ec4147b73cae550a38337520fc028dd", + "sarscov2.gt.stat.yml:md5,2504b472be03bdebaa033b0e507e4bfe" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-10T14:46:59.600343" + } +} \ No newline at end of file diff --git a/tests/tiny/params.json b/tests/tiny/params.json deleted file mode 100644 index 71262048..00000000 --- a/tests/tiny/params.json +++ /dev/null @@ -1,5 +0,0 @@ -{ - "config_profile_name": "Test profile", - "config_profile_description": "Tiny test dataset to check pipeline function", - "input": "tests/tiny/assemblysheet.csv" -} From 64d0cabcaef100b3f197e0f2c8a14368ace3ae52 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 19:35:52 +1300 Subject: [PATCH 30/62] Added missing modules --- .github/workflows/ci.yml | 12 +- .nf-core.yml | 1 + modules.json | 10 + modules/gallvp/gunzip/environment.yml | 7 + modules/gallvp/gunzip/main.nf | 55 ++ modules/gallvp/gunzip/meta.yml | 47 ++ modules/gallvp/gunzip/tests/main.nf.test | 121 +++++ modules/gallvp/gunzip/tests/main.nf.test.snap | 134 +++++ modules/gallvp/gunzip/tests/nextflow.config | 5 + modules/gallvp/gunzip/tests/tags.yml | 2 + modules/gallvp/minimap2/align/environment.yml | 8 + modules/gallvp/minimap2/align/main.nf | 78 +++ modules/gallvp/minimap2/align/meta.yml | 99 ++++ .../gallvp/minimap2/align/tests/main.nf.test | 441 ++++++++++++++++ .../minimap2/align/tests/main.nf.test.snap | 476 ++++++++++++++++++ modules/gallvp/minimap2/align/tests/tags.yml | 2 + 16 files changed, 1490 insertions(+), 8 deletions(-) create mode 100644 modules/gallvp/gunzip/environment.yml create mode 100644 modules/gallvp/gunzip/main.nf create mode 100644 modules/gallvp/gunzip/meta.yml create mode 100644 modules/gallvp/gunzip/tests/main.nf.test create mode 100644 modules/gallvp/gunzip/tests/main.nf.test.snap create mode 100644 modules/gallvp/gunzip/tests/nextflow.config create mode 100644 modules/gallvp/gunzip/tests/tags.yml create mode 100644 modules/gallvp/minimap2/align/environment.yml create mode 100644 modules/gallvp/minimap2/align/main.nf create mode 100644 modules/gallvp/minimap2/align/meta.yml create mode 100644 modules/gallvp/minimap2/align/tests/main.nf.test create mode 100644 modules/gallvp/minimap2/align/tests/main.nf.test.snap create mode 100644 modules/gallvp/minimap2/align/tests/tags.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index f920d3b9..5f35a995 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -5,7 +5,6 @@ on: env: NXF_ANSI_LOG: false - NFT_VER: "0.9.0" NFT_WORKDIR: "~" NFT_DIFF: "pdiff" NFT_DIFF_ARGS: "--line-numbers --expand-tabs=2" @@ -71,21 +70,18 @@ jobs: pip install pdiff - uses: nf-core/setup-nf-test@v1.1.2 + with: + version: 0.9.0 - name: Run nf-test run: | - nf-test test --verbose ${{ matrix.nf_test_files }} --profile "+${{ matrix.profile }}" --tap=test.tap - - - uses: pcolby/tap-summary@v1.1.1 - with: - path: >- - test.tap + nf-test test --verbose ${{ matrix.nf_test_files }} --profile "+${{ matrix.profile }}" - name: Output log on failure if: failure() run: | sudo apt install bat > /dev/null - batcat --decorations=always --color=always ${{ github.workspace }}/.nf-test/tests/*/meta/nextflow.log + batcat --decorations=always --color=always ${{ env.NFT_WORKDIR }}/tests/*/meta/nextflow.log confirm-pass: runs-on: ubuntu-latest diff --git a/.nf-core.yml b/.nf-core.yml index e167d7a8..a379021b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -20,6 +20,7 @@ lint: - docs/README.md multiqc_config: false template_strings: false + actions_ci: false nextflow_config: - manifest.name - manifest.homePage diff --git a/modules.json b/modules.json index e5e4e979..f44be8f8 100644 --- a/modules.json +++ b/modules.json @@ -65,6 +65,11 @@ "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["gff3_gt_gff3_gff3validator_stat"] }, + "gunzip": { + "branch": "main", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", + "installed_by": ["modules"] + }, "ltrfinder": { "branch": "main", "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", @@ -85,6 +90,11 @@ "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", "installed_by": ["fasta_ltrretriever_lai"] }, + "minimap2/align": { + "branch": "main", + "git_sha": "ae9714c21ede9199a3118e3c20b65484aa73e232", + "installed_by": ["modules"] + }, "plotsr": { "branch": "main", "git_sha": "a8939d36280e7d9037c7cf164eeede19e46546a4", diff --git a/modules/gallvp/gunzip/environment.yml b/modules/gallvp/gunzip/environment.yml new file mode 100644 index 00000000..c7794856 --- /dev/null +++ b/modules/gallvp/gunzip/environment.yml @@ -0,0 +1,7 @@ +channels: + - conda-forge + - bioconda +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/gallvp/gunzip/main.nf b/modules/gallvp/gunzip/main.nf new file mode 100644 index 00000000..5e67e3b9 --- /dev/null +++ b/modules/gallvp/gunzip/main.nf @@ -0,0 +1,55 @@ +process GUNZIP { + tag "$archive" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:22.04' : + 'nf-core/ubuntu:22.04' }" + + input: + tuple val(meta), path(archive) + + output: + tuple val(meta), path("$gunzip"), emit: gunzip + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def extension = ( archive.toString() - '.gz' ).tokenize('.')[-1] + def name = archive.toString() - '.gz' - ".$extension" + def prefix = task.ext.prefix ?: name + gunzip = prefix + ".$extension" + """ + # Not calling gunzip itself because it creates files + # with the original group ownership rather than the + # default one for that user / the work directory + gzip \\ + -cd \\ + $args \\ + $archive \\ + > $gunzip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def extension = ( archive.toString() - '.gz' ).tokenize('.')[-1] + def name = archive.toString() - '.gz' - ".$extension" + def prefix = task.ext.prefix ?: name + gunzip = prefix + ".$extension" + """ + touch $gunzip + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/gallvp/gunzip/meta.yml b/modules/gallvp/gunzip/meta.yml new file mode 100644 index 00000000..9066c035 --- /dev/null +++ b/modules/gallvp/gunzip/meta.yml @@ -0,0 +1,47 @@ +name: gunzip +description: Compresses and decompresses files. +keywords: + - gunzip + - compression + - decompression +tools: + - gunzip: + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html + licence: ["GPL-3.0-or-later"] + identifier: "" +input: + - - meta: + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be compressed/uncompressed + pattern: "*.*" +output: + - gunzip: + - meta: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - $gunzip: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" + - "@gallvp" diff --git a/modules/gallvp/gunzip/tests/main.nf.test b/modules/gallvp/gunzip/tests/main.nf.test new file mode 100644 index 00000000..f6610575 --- /dev/null +++ b/modules/gallvp/gunzip/tests/main.nf.test @@ -0,0 +1,121 @@ +nextflow_process { + + name "Test Process GUNZIP" + script "../main.nf" + process "GUNZIP" + tag "gunzip" + tag "modules_gallvp" + tag "modules" + + test("Should run without failures") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Should run without failures - prefix") { + + config './nextflow.config' + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id: 'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Should run without failures - stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Should run without failures - prefix - stub") { + + options '-stub' + config './nextflow.config' + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [ id: 'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/gallvp/gunzip/tests/main.nf.test.snap b/modules/gallvp/gunzip/tests/main.nf.test.snap new file mode 100644 index 00000000..069967e7 --- /dev/null +++ b/modules/gallvp/gunzip/tests/main.nf.test.snap @@ -0,0 +1,134 @@ +{ + "Should run without failures - prefix - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-25T11:35:10.861293" + }, + "Should run without failures - stub": { + "content": [ + { + "0": [ + [ + [ + + ], + "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + [ + + ], + "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-25T11:35:05.857145" + }, + "Should run without failures": { + "content": [ + { + "0": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2023-10-17T15:35:37.690477896" + }, + "Should run without failures - prefix": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + { + "id": "test" + }, + "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-25T11:33:32.921739" + } +} \ No newline at end of file diff --git a/modules/gallvp/gunzip/tests/nextflow.config b/modules/gallvp/gunzip/tests/nextflow.config new file mode 100644 index 00000000..dec77642 --- /dev/null +++ b/modules/gallvp/gunzip/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: GUNZIP { + ext.prefix = { "${meta.id}.xyz" } + } +} diff --git a/modules/gallvp/gunzip/tests/tags.yml b/modules/gallvp/gunzip/tests/tags.yml new file mode 100644 index 00000000..fd3f6915 --- /dev/null +++ b/modules/gallvp/gunzip/tests/tags.yml @@ -0,0 +1,2 @@ +gunzip: + - modules/nf-core/gunzip/** diff --git a/modules/gallvp/minimap2/align/environment.yml b/modules/gallvp/minimap2/align/environment.yml new file mode 100644 index 00000000..dc6476b7 --- /dev/null +++ b/modules/gallvp/minimap2/align/environment.yml @@ -0,0 +1,8 @@ +channels: + - conda-forge + - bioconda + +dependencies: + - bioconda::htslib=1.20 + - bioconda::minimap2=2.28 + - bioconda::samtools=1.20 diff --git a/modules/gallvp/minimap2/align/main.nf b/modules/gallvp/minimap2/align/main.nf new file mode 100644 index 00000000..d82dc14d --- /dev/null +++ b/modules/gallvp/minimap2/align/main.nf @@ -0,0 +1,78 @@ +process MINIMAP2_ALIGN { + tag "$meta.id" + label 'process_high' + + // Note: the versions here need to match the versions used in the mulled container below and minimap2/index + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3161f532a5ea6f1dec9be5667c9efc2afdac6104-0' : + 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3161f532a5ea6f1dec9be5667c9efc2afdac6104-0' }" + + input: + tuple val(meta), path(reads) + tuple val(meta2), path(reference) + val bam_format + val bam_index_extension + val cigar_paf_format + val cigar_bam + + output: + tuple val(meta), path("*.paf") , optional: true, emit: paf + tuple val(meta), path("*.bam") , optional: true, emit: bam + tuple val(meta), path("*.bam.${bam_index_extension}"), optional: true, emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def args3 = task.ext.args3 ?: '' + def args4 = task.ext.args4 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def bam_index = bam_index_extension ? "${prefix}.bam##idx##${prefix}.bam.${bam_index_extension} --write-index" : "${prefix}.bam" + def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus-1} -o ${bam_index} ${args2}" : "-o ${prefix}.paf" + def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' + def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' + def bam_input = "${reads.extension}".matches('sam|bam|cram') + def samtools_reset_fastq = bam_input ? "samtools reset --threads ${task.cpus-1} $args3 $reads | samtools fastq --threads ${task.cpus-1} $args4 |" : '' + def query = bam_input ? "-" : reads + def target = reference ?: (bam_input ? error("BAM input requires reference") : reads) + + """ + $samtools_reset_fastq \\ + minimap2 \\ + $args \\ + -t $task.cpus \\ + $target \\ + $query \\ + $cigar_paf \\ + $set_cigar_bam \\ + $bam_output + + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + minimap2: \$(minimap2 --version 2>&1) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def output_file = bam_format ? "${prefix}.bam" : "${prefix}.paf" + def bam_index = bam_index_extension ? "touch ${prefix}.bam.${bam_index_extension}" : "" + def bam_input = "${reads.extension}".matches('sam|bam|cram') + def target = reference ?: (bam_input ? error("BAM input requires reference") : reads) + + """ + touch $output_file + ${bam_index} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + minimap2: \$(minimap2 --version 2>&1) + END_VERSIONS + """ +} diff --git a/modules/gallvp/minimap2/align/meta.yml b/modules/gallvp/minimap2/align/meta.yml new file mode 100644 index 00000000..a4cfc891 --- /dev/null +++ b/modules/gallvp/minimap2/align/meta.yml @@ -0,0 +1,99 @@ +name: minimap2_align +description: A versatile pairwise aligner for genomic and spliced nucleotide sequences +keywords: + - align + - fasta + - fastq + - genome + - paf + - reference +tools: + - minimap2: + description: | + A versatile pairwise aligner for genomic and spliced nucleotide sequences. + homepage: https://github.com/lh3/minimap2 + documentation: https://github.com/lh3/minimap2#uguide + licence: ["MIT"] + identifier: "" +input: + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FASTA or FASTQ files of size 1 and 2 for single-end + and paired-end data, respectively. + - - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test_ref'] + - reference: + type: file + description: | + Reference database in FASTA format. + - - bam_format: + type: boolean + description: Specify that output should be in BAM format + - - bam_index_extension: + type: string + description: BAM alignment index extension (e.g. "bai") + - - cigar_paf_format: + type: boolean + description: Specify that output CIGAR should be in PAF format + - - cigar_bam: + type: boolean + description: | + Write CIGAR with >65535 ops at the CG tag. This is recommended when + doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations) +output: + - paf: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.paf": + type: file + description: Alignment in PAF format + pattern: "*.paf" + - bam: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.bam": + type: file + description: Alignment in BAM format + pattern: "*.bam" + - index: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.bam.${bam_index_extension}": + type: file + description: BAM alignment index + pattern: "*.bam.*" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@heuermh" + - "@sofstam" + - "@sateeshperi" + - "@jfy133" + - "@fellen31" +maintainers: + - "@heuermh" + - "@sofstam" + - "@sateeshperi" + - "@jfy133" + - "@fellen31" diff --git a/modules/gallvp/minimap2/align/tests/main.nf.test b/modules/gallvp/minimap2/align/tests/main.nf.test new file mode 100644 index 00000000..17510cb7 --- /dev/null +++ b/modules/gallvp/minimap2/align/tests/main.nf.test @@ -0,0 +1,441 @@ +nextflow_process { + + name "Test Process MINIMAP2_ALIGN" + script "../main.nf" + process "MINIMAP2_ALIGN" + + tag "modules" + tag "modules_gallvp" + tag "minimap2" + tag "minimap2/align" + + test("sarscov2 - fastq, fasta, true, [], false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, true, 'bai', false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + file(process.out.index[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], fasta, true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, [], true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - bam, fasta, true, [], false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - bam, fasta, true, 'bai', false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + bam(process.out.bam[0][1]).getHeader(), + bam(process.out.bam[0][1]).getReadsMD5(), + file(process.out.index[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - bam, [], true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.failed } + ) + } + + } + + test("sarscov2 - fastq, fasta, true, [], false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, true, 'bai', false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, false, [], false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = false + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam, fasta, true, [], false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam, fasta, true, 'bai', false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + input[2] = true + input[3] = 'bai' + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam, [], true, false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = [] + input[4] = false + input[5] = false + """ + } + } + + then { + assertAll( + { assert process.failed } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/gallvp/minimap2/align/tests/main.nf.test.snap b/modules/gallvp/minimap2/align/tests/main.nf.test.snap new file mode 100644 index 00000000..12264a85 --- /dev/null +++ b/modules/gallvp/minimap2/align/tests/main.nf.test.snap @@ -0,0 +1,476 @@ +{ + "sarscov2 - 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fastq, [], true, false, false": { + "content": [ + [ + "@HD\tVN:1.6\tSO:coordinate", + "@SQ\tSN:ERR5069949.2151832\tLN:150", + "@SQ\tSN:ERR5069949.576388\tLN:77", + "@SQ\tSN:ERR5069949.501486\tLN:146", + "@SQ\tSN:ERR5069949.1331889\tLN:132", + "@SQ\tSN:ERR5069949.2161340\tLN:80", + "@SQ\tSN:ERR5069949.973930\tLN:79", + "@SQ\tSN:ERR5069949.2417063\tLN:150", + "@SQ\tSN:ERR5069949.376959\tLN:151", + "@SQ\tSN:ERR5069949.1088785\tLN:149", + "@SQ\tSN:ERR5069949.1066259\tLN:147", + "@SQ\tSN:ERR5069949.2832676\tLN:139", + "@SQ\tSN:ERR5069949.2953930\tLN:151", + "@SQ\tSN:ERR5069949.324865\tLN:151", + "@SQ\tSN:ERR5069949.2185111\tLN:150", + "@SQ\tSN:ERR5069949.937422\tLN:151", + "@SQ\tSN:ERR5069949.2431709\tLN:150", + "@SQ\tSN:ERR5069949.1246538\tLN:148", + "@SQ\tSN:ERR5069949.1189252\tLN:98", + "@SQ\tSN:ERR5069949.2216307\tLN:147", + "@SQ\tSN:ERR5069949.3273002\tLN:148", + "@SQ\tSN:ERR5069949.3277445\tLN:151", + "@SQ\tSN:ERR5069949.3022231\tLN:147", + "@SQ\tSN:ERR5069949.184542\tLN:151", + "@SQ\tSN:ERR5069949.540529\tLN:149", + "@SQ\tSN:ERR5069949.686090\tLN:150", + "@SQ\tSN:ERR5069949.2787556\tLN:106", + "@SQ\tSN:ERR5069949.2650879\tLN:150", + "@SQ\tSN:ERR5069949.2064910\tLN:149", + "@SQ\tSN:ERR5069949.2328704\tLN:150", + "@SQ\tSN:ERR5069949.1067032\tLN:150", + "@SQ\tSN:ERR5069949.3338256\tLN:151", + "@SQ\tSN:ERR5069949.1412839\tLN:147", + "@SQ\tSN:ERR5069949.1538968\tLN:150", + "@SQ\tSN:ERR5069949.147998\tLN:94", + "@SQ\tSN:ERR5069949.366975\tLN:106", + "@SQ\tSN:ERR5069949.1372331\tLN:151", + "@SQ\tSN:ERR5069949.1709367\tLN:129", + "@SQ\tSN:ERR5069949.2388984\tLN:150", + "@SQ\tSN:ERR5069949.1132353\tLN:150", + "@SQ\tSN:ERR5069949.1151736\tLN:151", + "@SQ\tSN:ERR5069949.479807\tLN:150", + "@SQ\tSN:ERR5069949.2176303\tLN:151", + "@SQ\tSN:ERR5069949.2772897\tLN:151", + "@SQ\tSN:ERR5069949.1020777\tLN:122", + "@SQ\tSN:ERR5069949.465452\tLN:151", + "@SQ\tSN:ERR5069949.1704586\tLN:149", + "@SQ\tSN:ERR5069949.1258508\tLN:151", + "@SQ\tSN:ERR5069949.986441\tLN:119", + "@SQ\tSN:ERR5069949.2674295\tLN:148", + "@SQ\tSN:ERR5069949.885966\tLN:79", + "@SQ\tSN:ERR5069949.2342766\tLN:151", + "@SQ\tSN:ERR5069949.3122970\tLN:127", + "@SQ\tSN:ERR5069949.3279513\tLN:72", + "@SQ\tSN:ERR5069949.309410\tLN:151", + "@SQ\tSN:ERR5069949.532979\tLN:149", + "@SQ\tSN:ERR5069949.2888794\tLN:151", + "@SQ\tSN:ERR5069949.2205229\tLN:150", + "@SQ\tSN:ERR5069949.786562\tLN:151", + "@SQ\tSN:ERR5069949.919671\tLN:151", + "@SQ\tSN:ERR5069949.1328186\tLN:151", + "@SQ\tSN:ERR5069949.870926\tLN:149", + "@SQ\tSN:ERR5069949.2257580\tLN:151", + "@SQ\tSN:ERR5069949.3249622\tLN:77", + "@SQ\tSN:ERR5069949.611123\tLN:125", + "@SQ\tSN:ERR5069949.651338\tLN:142", + "@SQ\tSN:ERR5069949.169513\tLN:92", + "@SQ\tSN:ERR5069949.155944\tLN:150", + "@SQ\tSN:ERR5069949.2033605\tLN:150", + "@SQ\tSN:ERR5069949.2730382\tLN:142", + "@SQ\tSN:ERR5069949.2125592\tLN:150", + "@SQ\tSN:ERR5069949.1062611\tLN:151", + "@SQ\tSN:ERR5069949.1778133\tLN:151", + "@SQ\tSN:ERR5069949.3057020\tLN:95", + "@SQ\tSN:ERR5069949.2972968\tLN:141", + "@SQ\tSN:ERR5069949.2734474\tLN:149", + "@SQ\tSN:ERR5069949.856527\tLN:151", + "@SQ\tSN:ERR5069949.2098070\tLN:151", + "@SQ\tSN:ERR5069949.1552198\tLN:150", + "@SQ\tSN:ERR5069949.2385514\tLN:150", + "@SQ\tSN:ERR5069949.2270078\tLN:151", + "@SQ\tSN:ERR5069949.114870\tLN:150", + "@SQ\tSN:ERR5069949.2668880\tLN:147", + "@SQ\tSN:ERR5069949.257821\tLN:139", + "@SQ\tSN:ERR5069949.2243023\tLN:150", + "@SQ\tSN:ERR5069949.2605155\tLN:146", + "@SQ\tSN:ERR5069949.1340552\tLN:151", + "@SQ\tSN:ERR5069949.1561137\tLN:150", + "@SQ\tSN:ERR5069949.2361683\tLN:149", + "@SQ\tSN:ERR5069949.2521353\tLN:150", + "@SQ\tSN:ERR5069949.1261808\tLN:149", + "@SQ\tSN:ERR5069949.2734873\tLN:98", + "@SQ\tSN:ERR5069949.3017828\tLN:107", + "@SQ\tSN:ERR5069949.573706\tLN:150", + "@SQ\tSN:ERR5069949.1980512\tLN:151", + "@SQ\tSN:ERR5069949.1014693\tLN:150", + "@SQ\tSN:ERR5069949.3184655\tLN:150", + "@SQ\tSN:ERR5069949.29668\tLN:89", + "@SQ\tSN:ERR5069949.3258358\tLN:151", + "@SQ\tSN:ERR5069949.1476386\tLN:151", + "@SQ\tSN:ERR5069949.2415814\tLN:150", + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a test_1.fastq.gz test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + ], + "16c1c651f8ec67383bcdee3c55aed94f", + [ + "versions.yml:md5,3548eeba9066efbf8d78ea99f8d813fd" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-23T11:18:34.246998277" + } +} \ No newline at end of file diff --git a/modules/gallvp/minimap2/align/tests/tags.yml b/modules/gallvp/minimap2/align/tests/tags.yml new file mode 100644 index 00000000..39dba374 --- /dev/null +++ b/modules/gallvp/minimap2/align/tests/tags.yml @@ -0,0 +1,2 @@ +minimap2/align: + - "modules/nf-core/minimap2/align/**" From b193a4c156c193e21e6b79f14543552a8a11245d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 20:00:07 +1300 Subject: [PATCH 31/62] Updated config selectors --- .github/workflows/ci.yml | 6 ---- conf/modules.config | 68 ++++++++++++++++++++-------------------- 2 files changed, 34 insertions(+), 40 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 5f35a995..b9fdd842 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -77,12 +77,6 @@ jobs: run: | nf-test test --verbose ${{ matrix.nf_test_files }} --profile "+${{ matrix.profile }}" - - name: Output log on failure - if: failure() - run: | - sudo apt install bat > /dev/null - batcat --decorations=always --color=always ${{ env.NFT_WORKDIR }}/tests/*/meta/nextflow.log - confirm-pass: runs-on: ubuntu-latest needs: [test] diff --git a/conf/modules.config b/conf/modules.config index efc06589..c1e8113a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -12,7 +12,7 @@ process { - withName: SEQKIT_RMDUP { + withName: '.*:ASSEMBLYQC:SEQKIT_RMDUP' { ext.args = '--by-seq --ignore-case' ext.prefix = { "${meta.id}.seqkit.rmdup" } } @@ -42,7 +42,7 @@ process { ] } - withName: ASSEMBLATHON_STATS { + withName: '.*:ASSEMBLYQC:ASSEMBLATHON_STATS' { publishDir = [ path: { "${params.outdir}/assemblathon_stats" }, mode: params.publish_dir_mode, @@ -50,7 +50,7 @@ process { ] } - withName: GFASTATS { + withName: '.*:ASSEMBLYQC:GFASTATS' { ext.args = '--stats -t --nstar-report' publishDir = [ path: { "${params.outdir}/gfastats" }, @@ -60,7 +60,7 @@ process { ] } - withName: FCS_FCSADAPTOR { + withName: '.*:ASSEMBLYQC:FCS_FCSADAPTOR' { ext.args = params.ncbi_fcs_adaptor_empire ? "--${params.ncbi_fcs_adaptor_empire}" : '--prok' publishDir = [ @@ -70,7 +70,7 @@ process { ] } - withName: NCBI_FCS_GX_SCREEN_SAMPLES { + withName: '.*:NCBI_FCS_GX:NCBI_FCS_GX_SCREEN_SAMPLES' { publishDir = [ path: { "${params.outdir}/ncbi_fcs_gx" }, mode: params.publish_dir_mode, @@ -78,7 +78,7 @@ process { ] } - withName: NCBI_FCS_GX_KRONA_PLOT { + withName: '.*:NCBI_FCS_GX:NCBI_FCS_GX_KRONA_PLOT' { publishDir = [ path: { "${params.outdir}/ncbi_fcs_gx" }, mode: params.publish_dir_mode, @@ -128,7 +128,7 @@ process { ] } - withName: KRAKEN2 { + withName: '.*:FASTA_KRAKEN2:KRAKEN2' { publishDir = [ path: { "${params.outdir}/kraken2" }, mode: params.publish_dir_mode, @@ -136,7 +136,7 @@ process { ] } - withName: KRAKEN2_KRONA_PLOT { + withName: '.*:FASTA_KRAKEN2:KRAKEN2_KRONA_PLOT' { publishDir = [ path: { "${params.outdir}/kraken2" }, mode: params.publish_dir_mode, @@ -144,7 +144,7 @@ process { ] } - withName: CIRCOS { + withName: '.*:FASTA_SYNTENY:CIRCOS' { publishDir = [ path: { "${params.outdir}/synteny/${target_on_ref_seq}" }, mode: params.publish_dir_mode, @@ -152,7 +152,7 @@ process { ] } - withName: LINEARSYNTENY { + withName: '.*:FASTA_SYNTENY:LINEARSYNTENY' { publishDir = [ path: { "${params.outdir}/synteny/${target_on_ref_seq}" }, mode: params.publish_dir_mode, @@ -164,7 +164,7 @@ process { ext.prefix = { "${meta.id}.plotsr" } } - withName: MINIMAP2_ALIGN { + withName: '.*:FASTA_SYNTENY:MINIMAP2_ALIGN' { ext.args = '-x asm5 --eqx' } @@ -187,17 +187,17 @@ process { ] } - withName: FILTER_BY_LENGTH { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:FILTER_BY_LENGTH' { ext.args = params.tidk_filter_by_size ? "-m ${params.tidk_filter_size_bp}" : '' ext.prefix = { "${meta.id}.filtered" } } - withName: SORT_BY_LENGTH { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:SORT_BY_LENGTH' { ext.args = '--quiet --reverse --by-length' ext.prefix = { "${meta.id}.sorted" } } - withName: TIDK_EXPLORE { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:TIDK_EXPLORE' { ext.args = '--minimum 5 --maximum 30' publishDir = [ path: { "${params.outdir}/tidk" }, @@ -206,7 +206,7 @@ process { ] } - withName: TIDK_SEARCH_APRIORI { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:TIDK_SEARCH_APRIORI' { ext.prefix = { "${meta.id}.apriori" } ext.args = '--extension tsv' publishDir = [ @@ -216,7 +216,7 @@ process { ] } - withName: TIDK_SEARCH_APOSTERIORI { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:TIDK_SEARCH_APOSTERIORI' { ext.prefix = { "${meta.id}.aposteriori" } ext.args = '--extension tsv' publishDir = [ @@ -226,7 +226,7 @@ process { ] } - withName: TIDK_PLOT_APRIORI { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:TIDK_PLOT_APRIORI' { ext.prefix = { "${meta.id}.apriori" } publishDir = [ path: { "${params.outdir}/tidk" }, @@ -235,7 +235,7 @@ process { ] } - withName: TIDK_PLOT_APOSTERIORI { + withName: '.*:FASTA_EXPLORE_SEARCH_PLOT_TIDK:TIDK_PLOT_APOSTERIORI' { ext.prefix = { "${meta.id}.aposteriori" } publishDir = [ path: { "${params.outdir}/tidk" }, @@ -248,7 +248,7 @@ process { ext.args = '-u' } - withName: CUSTOM_SHORTENFASTAIDS { + withName: '.*:FASTA_LTRRETRIEVER_LAI:CUSTOM_SHORTENFASTAIDS' { publishDir = [ path: { "${params.outdir}/lai" }, mode: params.publish_dir_mode, @@ -256,11 +256,11 @@ process { ] } - withName: LTRHARVEST { + withName: '.*:FASTA_LTRRETRIEVER_LAI:LTRHARVEST' { ext.prefix = { "${meta.id}_ltrharvest" } } - withName: LTRFINDER { + withName: '.*:FASTA_LTRRETRIEVER_LAI:LTRFINDER' { ext.args = '-harvest_out -size 1000000 -time 300' } @@ -268,7 +268,7 @@ process { ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } } - withName: LTRRETRIEVER_LTRRETRIEVER { + withName: '.*:FASTA_LTRRETRIEVER_LAI:LTRRETRIEVER_LTRRETRIEVER' { publishDir = [ path: { "${params.outdir}/lai" }, mode: params.publish_dir_mode, @@ -276,7 +276,7 @@ process { ] } - withName: CUSTOM_RESTOREGFFIDS { + withName: '.*:FASTA_LTRRETRIEVER_LAI:CUSTOM_RESTOREGFFIDS' { publishDir = [ path: { "${params.outdir}/lai" }, mode: params.publish_dir_mode, @@ -284,7 +284,7 @@ process { ] } - withName: LTRRETRIEVER_LAI { + withName: '.*:FASTA_LTRRETRIEVER_LAI:LTRRETRIEVER_LAI' { publishDir = [ path: { "${params.outdir}/lai" }, mode: params.publish_dir_mode, @@ -321,17 +321,17 @@ process { ext.args = '--ignore-case --natural-order' } - withName: BWA_MEM { + withName: '.*:FASTQ_BWA_MEM_SAMBLASTER:BWA_MEM' { ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } ext.args = '-5SP' } - withName: SAMBLASTER { + withName: '.*:FASTQ_BWA_MEM_SAMBLASTER:SAMBLASTER' { ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } ext.args3 = '-h -F 2316' } - withName: AGP2ASSEMBLY { + withName: '.*:FQ2HIC:AGP2ASSEMBLY' { publishDir = [ path: { "${params.outdir}/hic/assembly" }, mode: params.publish_dir_mode, @@ -339,7 +339,7 @@ process { ] } - withName: ASSEMBLY2BEDPE { + withName: '.*:FQ2HIC:ASSEMBLY2BEDPE' { publishDir = [ path: { "${params.outdir}/hic/bedpe" }, mode: params.publish_dir_mode, @@ -347,7 +347,7 @@ process { ] } - withName: HIC2HTML { + withName: '.*:FQ2HIC:HIC2HTML' { publishDir = [ path: { "${params.outdir}/hic" }, mode: params.publish_dir_mode, @@ -355,7 +355,7 @@ process { ] } - withName: HICQC { + withName: '.*:FQ2HIC:HICQC' { publishDir = [ path: { "${params.outdir}/hic/hicqc" }, mode: params.publish_dir_mode, @@ -363,7 +363,7 @@ process { ] } - withName: RUNASSEMBLYVISUALIZER { + withName: '.*:FQ2HIC:RUNASSEMBLYVISUALIZER' { publishDir = [ path: { "${params.outdir}/hic" }, mode: params.publish_dir_mode, @@ -371,11 +371,11 @@ process { ] } - withName: TAG_ASSEMBLY { + withName: '.*:ASSEMBLYQC:TAG_ASSEMBLY' { ext.prefix = { "${meta.id}.fasta" } } - withName: MERQURY_MERQURY { + withName: '.*:ASSEMBLYQC:MERQURY_MERQURY' { publishDir = [ [ path: { "${params.outdir}/merqury/${meta.id}" }, @@ -385,7 +385,7 @@ process { ] } - withName: CREATEREPORT { + withName: '.*:ASSEMBLYQC:CREATEREPORT' { publishDir = [ path: { "$params.outdir" }, mode: params.publish_dir_mode, From beb0c7be9634fb20da4e2acb4623fac590eb1802 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 21:11:10 +1300 Subject: [PATCH 32/62] Updated snapshots to account for 4 CPUs --- .github/workflows/ci.yml | 4 ++ conf/modules.config | 10 +-- .../gallvp/bwa/mem/tests/main.nf.test.snap | 20 +++--- .../minimap2/align/tests/main.nf.test.snap | 60 ++++++++-------- modules/nf-core/fastp/tests/main.nf.test.snap | 72 +++++++++---------- .../minimap2/align/tests/main.nf.test.snap | 60 ++++++++-------- .../tests/main.nf.test.snap | 10 +-- .../tests/main.nf.test.snap | 48 ++++++------- tests/noltr/main.nf.test | 8 ++- tests/noltr/main.nf.test.snap | 4 +- 10 files changed, 152 insertions(+), 144 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index b9fdd842..0b4c607d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -73,6 +73,10 @@ jobs: with: version: 0.9.0 + - name: Disk space cleanup + if: matrix.nf_test_files == 'tests/stub/main.nf.test' + uses: jlumbroso/free-disk-space@v1.3.1 + - name: Run nf-test run: | nf-test test --verbose ${{ matrix.nf_test_files }} --profile "+${{ matrix.profile }}" diff --git a/conf/modules.config b/conf/modules.config index c1e8113a..24722302 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -292,7 +292,7 @@ process { ] } - withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_RAW' { + withName: '.*:FQ2HIC:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_RAW' { publishDir = [ path: { "${params.outdir}/hic/fastqc_raw" }, mode: params.publish_dir_mode, @@ -300,7 +300,7 @@ process { ] } - withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' { + withName: '.*:FQ2HIC:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' { publishDir = [ path: { "${params.outdir}/hic/fastqc_trim" }, mode: params.publish_dir_mode, @@ -308,7 +308,7 @@ process { ] } - withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTP' { + withName: '.*:FQ2HIC:FASTQ_FASTQC_UMITOOLS_FASTP:FASTP' { ext.args = params.hic_fastp_ext_args publishDir = [ path: { "${params.outdir}/hic/fastp" }, @@ -321,12 +321,12 @@ process { ext.args = '--ignore-case --natural-order' } - withName: '.*:FASTQ_BWA_MEM_SAMBLASTER:BWA_MEM' { + withName: '.*:FQ2HIC:FASTQ_BWA_MEM_SAMBLASTER:BWA_MEM' { ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } ext.args = '-5SP' } - withName: '.*:FASTQ_BWA_MEM_SAMBLASTER:SAMBLASTER' { + withName: '.*:FQ2HIC:FASTQ_BWA_MEM_SAMBLASTER:SAMBLASTER' { ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } ext.args3 = '-h -F 2316' } diff --git a/modules/gallvp/bwa/mem/tests/main.nf.test.snap b/modules/gallvp/bwa/mem/tests/main.nf.test.snap index 2079ea22..5b456333 100644 --- a/modules/gallvp/bwa/mem/tests/main.nf.test.snap +++ b/modules/gallvp/bwa/mem/tests/main.nf.test.snap @@ -13,14 +13,14 @@ [ "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" ], - "b6d9cb250261a4c125413c5d867d87a7", + "895cf107b1c13b0f0cabc1421a4f5db9", "798439cbd7fd81cbcc5078022dc5479d" ], "meta": { "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-08-02T12:22:28.051598" + "timestamp": "2024-10-10T20:57:38.428887" }, "Single-End Sort": { "content": [ @@ -36,14 +36,14 @@ [ "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" ], - "848434ae4b79cfdcb2281c60b33663ce", + "98350949b5a0931930c69cff0e380274", "94fcf617f5b994584c4e8d4044e16b4f" ], "meta": { "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-08-02T12:22:39.671154" + "timestamp": "2024-10-10T20:57:52.438702" }, "Paired-End": { "content": [ @@ -59,14 +59,14 @@ [ "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" ], - "5b34d31be84478761f789e3e2e805e31", + "a42d8e219e0df24f3d72a646b7c26dbb", "57aeef88ed701a8ebc8e2f0a381b2a6" ], "meta": { "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-08-02T12:22:51.919479" + "timestamp": "2024-10-10T20:58:05.532827" }, "Paired-End Sort": { "content": [ @@ -82,14 +82,14 @@ [ "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" ], - "69003376d9a8952622d8587b39c3eaae", + "ee5c324f68d45c4d01c937b4abbc5de", "af8628d9df18b2d3d4f6fd47ef2bb872" ], "meta": { "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-08-02T12:23:00.833562" + "timestamp": "2024-10-10T20:58:20.859431" }, "Single-end - stub": { "content": [ @@ -182,14 +182,14 @@ [ "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" ], - "5b34d31be84478761f789e3e2e805e31", + "a42d8e219e0df24f3d72a646b7c26dbb", "57aeef88ed701a8ebc8e2f0a381b2a6" ], "meta": { "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-08-02T12:23:09.942545" + "timestamp": "2024-10-10T20:58:35.460705" }, "Paired-end - stub": { "content": [ diff --git a/modules/gallvp/minimap2/align/tests/main.nf.test.snap b/modules/gallvp/minimap2/align/tests/main.nf.test.snap index 12264a85..bafaabd7 100644 --- a/modules/gallvp/minimap2/align/tests/main.nf.test.snap +++ b/modules/gallvp/minimap2/align/tests/main.nf.test.snap @@ -4,8 +4,8 @@ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta -", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta -", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam##idx##test.bam.bai --write-index" ], "5d426b9a5f5b2c54f1d7f1e4c238ae94", "test.bam.bai", @@ -14,10 +14,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-25T09:03:00.827260362" + "timestamp": "2024-10-10T20:47:38.40726" }, "sarscov2 - bam, fasta, true, 'bai', false, false - stub": { "content": [ @@ -236,8 +236,8 @@ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz test_2.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta test_1.fastq.gz test_2.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "1bc392244f228bf52cf0b5a8f6a654c9", [ @@ -245,18 +245,18 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:18:18.964586894" + "timestamp": "2024-10-10T20:47:04.155509" }, "sarscov2 - fastq, fasta, true, [], false, false": { "content": [ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "f194745c0ccfcb2a9c0aee094a08750", [ @@ -264,18 +264,18 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:17:48.667488325" + "timestamp": "2024-10-10T20:46:42.073683" }, "sarscov2 - fastq, fasta, true, 'bai', false, false": { "content": [ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam##idx##test.bam.bai --write-index" ], "f194745c0ccfcb2a9c0aee094a08750", "test.bam.bai", @@ -284,18 +284,18 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:18:02.517416733" + "timestamp": "2024-10-10T20:46:53.814566" }, "sarscov2 - bam, fasta, true, [], false, false": { "content": [ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta -", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta -", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "5d426b9a5f5b2c54f1d7f1e4c238ae94", [ @@ -303,10 +303,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-25T09:02:49.64829488" + "timestamp": "2024-10-10T20:47:26.993111" }, "sarscov2 - bam, fasta, true, [], false, false - stub": { "content": [ @@ -459,8 +459,8 @@ "@SQ\tSN:ERR5069949.3258358\tLN:151", "@SQ\tSN:ERR5069949.1476386\tLN:151", "@SQ\tSN:ERR5069949.2415814\tLN:150", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a test_1.fastq.gz test_1.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a test_1.fastq.gz test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "16c1c651f8ec67383bcdee3c55aed94f", [ @@ -468,9 +468,9 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:18:34.246998277" + "timestamp": "2024-10-10T20:47:14.585958" } } \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap index 54be7e45..5561ac62 100644 --- a/modules/nf-core/fastp/tests/main.nf.test.snap +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -96,7 +96,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + "test.fastp.json:md5,63273f642b5a4495ce7ccba2d4419edf" ] ], [ @@ -122,10 +122,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:43:28.665779" + "timestamp": "2024-10-10T20:59:33.270219" }, "test_fastp_paired_end_merged_adapterlist": { "content": [ @@ -135,7 +135,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,5914ca3f21ce162123a824e33e8564f6" + "test.fastp.json:md5,7412a2cca178cc149757b229d2730d24" ] ], [ @@ -167,10 +167,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:44:18.210375" + "timestamp": "2024-10-10T21:00:25.951101" }, "test_fastp_single_end_qc_only": { "content": [ @@ -180,7 +180,7 @@ "id": "test", "single_end": true }, - "test.fastp.json:md5,5cc5f01e449309e0e689ed6f51a2294a" + "test.fastp.json:md5,8117ddbdb1ae856821eb66f40b14ba05" ] ], [ @@ -206,10 +206,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:44:27.380974" + "timestamp": "2024-10-10T21:00:36.271913" }, "test_fastp_paired_end_trim_fail": { "content": [ @@ -247,7 +247,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,4c3268ddb50ea5b33125984776aa3519" + "test.fastp.json:md5,5b7664268c0537423ffaa162701dae70" ] ], [ @@ -255,10 +255,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:43:58.749589" + "timestamp": "2024-10-10T21:00:04.189799" }, "fastp - stub test_fastp_interleaved": { "content": [ @@ -708,7 +708,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,b712fd68ed0322f4bec49ff2a5237fcc" + "test.fastp.json:md5,defba10ab9bb3e4235a86d90a51a2e79" ] ], [ @@ -740,10 +740,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:44:08.68476" + "timestamp": "2024-10-10T21:00:13.75505" }, "test_fastp_paired_end - stub": { "content": [ @@ -860,7 +860,7 @@ "id": "test", "single_end": true }, - "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" + "test.fastp.json:md5,898dafe80449209d752985698a8cded0" ] ], [ @@ -883,10 +883,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:43:18.834322" + "timestamp": "2024-10-10T20:59:23.552733" }, "test_fastp_single_end_trim_fail - stub": { "content": [ @@ -1145,7 +1145,7 @@ "id": "test", "single_end": true }, - "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22" + "test.fastp.json:md5,d09c3ec938c4a7bd04bd6398f42fa9ca" ] ], [ @@ -1153,10 +1153,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:43:38.910832" + "timestamp": "2024-10-10T20:59:41.725562" }, "test_fastp_single_end_trim_fail": { "content": [ @@ -1166,7 +1166,7 @@ "id": "test", "single_end": true }, - "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5" + "test.fastp.json:md5,4415a44b64ff54a7a450b0acec32bc46" ] ], [ @@ -1195,10 +1195,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:43:48.22378" + "timestamp": "2024-10-10T20:59:52.36865" }, "test_fastp_paired_end_qc_only": { "content": [ @@ -1208,7 +1208,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,623064a45912dac6f2b64e3f2e9901df" + "test.fastp.json:md5,6ad01e6f6a98daf02410273af0b8ecea" ] ], [ @@ -1234,10 +1234,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-05T13:44:36.334938" + "timestamp": "2024-10-10T21:00:46.609595" }, "test_fastp_paired_end_qc_only - stub": { "content": [ diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap index 12264a85..bafaabd7 100644 --- a/modules/nf-core/minimap2/align/tests/main.nf.test.snap +++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap @@ -4,8 +4,8 @@ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta -", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta -", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam##idx##test.bam.bai --write-index" ], "5d426b9a5f5b2c54f1d7f1e4c238ae94", "test.bam.bai", @@ -14,10 +14,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-25T09:03:00.827260362" + "timestamp": "2024-10-10T20:47:38.40726" }, "sarscov2 - bam, fasta, true, 'bai', false, false - stub": { "content": [ @@ -236,8 +236,8 @@ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz test_2.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta test_1.fastq.gz test_2.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "1bc392244f228bf52cf0b5a8f6a654c9", [ @@ -245,18 +245,18 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:18:18.964586894" + "timestamp": "2024-10-10T20:47:04.155509" }, "sarscov2 - fastq, fasta, true, [], false, false": { "content": [ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "f194745c0ccfcb2a9c0aee094a08750", [ @@ -264,18 +264,18 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:17:48.667488325" + "timestamp": "2024-10-10T20:46:42.073683" }, "sarscov2 - fastq, fasta, true, 'bai', false, false": { "content": [ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam##idx##test.bam.bai --write-index" ], "f194745c0ccfcb2a9c0aee094a08750", "test.bam.bai", @@ -284,18 +284,18 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:18:02.517416733" + "timestamp": "2024-10-10T20:46:53.814566" }, "sarscov2 - bam, fasta, true, [], false, false": { "content": [ [ "@HD\tVN:1.6\tSO:coordinate", "@SQ\tSN:MT192765.1\tLN:29829", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a genome.fasta -", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a genome.fasta -", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "5d426b9a5f5b2c54f1d7f1e4c238ae94", [ @@ -303,10 +303,10 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-25T09:02:49.64829488" + "timestamp": "2024-10-10T20:47:26.993111" }, "sarscov2 - bam, fasta, true, [], false, false - stub": { "content": [ @@ -459,8 +459,8 @@ "@SQ\tSN:ERR5069949.3258358\tLN:151", "@SQ\tSN:ERR5069949.1476386\tLN:151", "@SQ\tSN:ERR5069949.2415814\tLN:150", - "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 2 -a test_1.fastq.gz test_1.fastq.gz", - "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 1 -o test.bam" + "@PG\tID:minimap2\tPN:minimap2\tVN:2.28-r1209\tCL:minimap2 -t 4 -a test_1.fastq.gz test_1.fastq.gz", + "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.20\tCL:samtools sort -@ 3 -o test.bam" ], "16c1c651f8ec67383bcdee3c55aed94f", [ @@ -468,9 +468,9 @@ ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-23T11:18:34.246998277" + "timestamp": "2024-10-10T20:47:14.585958" } } \ No newline at end of file diff --git a/subworkflows/gallvp/fastq_bwa_mem_samblaster/tests/main.nf.test.snap b/subworkflows/gallvp/fastq_bwa_mem_samblaster/tests/main.nf.test.snap index a2b24322..270a96d7 100644 --- a/subworkflows/gallvp/fastq_bwa_mem_samblaster/tests/main.nf.test.snap +++ b/subworkflows/gallvp/fastq_bwa_mem_samblaster/tests/main.nf.test.snap @@ -8,7 +8,7 @@ "id": "test", "ref_id": "genome" }, - "test.on.genome.samblaster.bam:md5,38ade4c8ae74d49a197286e09a6cb3ad" + "test.on.genome.samblaster.bam:md5,f1552dd0431bc22320567699a3a70893" ] ], "1": [ @@ -22,7 +22,7 @@ "id": "test", "ref_id": "genome" }, - "test.on.genome.samblaster.bam:md5,38ade4c8ae74d49a197286e09a6cb3ad" + "test.on.genome.samblaster.bam:md5,f1552dd0431bc22320567699a3a70893" ] ], "versions": [ @@ -33,10 +33,10 @@ } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-05-22T15:55:44.323082" + "timestamp": "2024-10-10T20:56:44.071963" }, "sarscov2-fq-gz-stub": { "content": [ diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap index e7d1f51e..1ea1ff6e 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap @@ -28,7 +28,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + "test.fastp.json:md5,63273f642b5a4495ce7ccba2d4419edf" ] ], [ @@ -55,9 +55,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:56:01.933832" + "timestamp": "2024-10-10T20:51:01.289498" }, "save_trimmed_fail": { "content": [ @@ -88,7 +88,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,4c3268ddb50ea5b33125984776aa3519" + "test.fastp.json:md5,5b7664268c0537423ffaa162701dae70" ] ], [ @@ -127,9 +127,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:57:38.736" + "timestamp": "2024-10-10T20:53:09.142373" }, "skip_umi_extract": { "content": [ @@ -160,7 +160,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + "test.fastp.json:md5,63273f642b5a4495ce7ccba2d4419edf" ] ], [ @@ -189,9 +189,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:56:47.905105" + "timestamp": "2024-10-10T20:52:17.865965" }, "umi_discard_read = 2": { "content": [ @@ -222,7 +222,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + "test.fastp.json:md5,63273f642b5a4495ce7ccba2d4419edf" ] ], [ @@ -251,9 +251,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:57:05.436744" + "timestamp": "2024-10-10T20:52:36.15093" }, "umi_discard_read = 2 - stub": { "content": [ @@ -569,7 +569,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,b712fd68ed0322f4bec49ff2a5237fcc" + "test.fastp.json:md5,defba10ab9bb3e4235a86d90a51a2e79" ] ], [ @@ -604,9 +604,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:57:57.472342" + "timestamp": "2024-10-10T20:53:27.17416" }, "skip_trimming": { "content": [ @@ -668,7 +668,7 @@ "id": "test", "single_end": true }, - "test.fastp.json:md5,d39c5c6d9a2e35fb60d26ced46569af6" + "test.fastp.json:md5,b8dca1b3f56429748ab1ee6b84d9880a" ] ], [ @@ -695,9 +695,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:56:26.778625" + "timestamp": "2024-10-10T20:51:59.583782" }, "min_trimmed_reads = 26": { "content": [ @@ -728,7 +728,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,b712fd68ed0322f4bec49ff2a5237fcc" + "test.fastp.json:md5,defba10ab9bb3e4235a86d90a51a2e79" ] ], [ @@ -763,9 +763,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:58:16.36697" + "timestamp": "2024-10-10T20:53:43.614487" }, "min_trimmed_reads = 26 - stub": { "content": [ @@ -1676,7 +1676,7 @@ "id": "test", "single_end": false }, - "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + "test.fastp.json:md5,63273f642b5a4495ce7ccba2d4419edf" ] ], [ @@ -1705,9 +1705,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T16:55:50.614571" + "timestamp": "2024-10-10T20:50:49.176138" }, "sarscov2 paired-end [fastq] - stub": { "content": [ diff --git a/tests/noltr/main.nf.test b/tests/noltr/main.nf.test index 98f9e1ce..b42eaa68 100644 --- a/tests/noltr/main.nf.test +++ b/tests/noltr/main.nf.test @@ -21,7 +21,9 @@ nextflow_pipeline { 'pipeline_info/*.{html,json,txt,yml}', 'report.{html,json}', 'lai/*.LAI.log', - 'lai/*.restored.ids.gff3' + 'lai/*.restored.ids.gff3', + 'lai/*.LAI.out', + 'lai/*.LTRlib.fa' ], null, ['**'] @@ -39,6 +41,10 @@ nextflow_pipeline { assertAll( { assert workflow.success}, + { + def lai = file("$outputDir/lai/FI1.LAI.out").readLines()[1].split('\t')[6] + assert Math.abs(lai - 4.84) <= 1.0 + }, { assert snapshot( [ 'successful tasks': workflow.trace.succeeded().size(), diff --git a/tests/noltr/main.nf.test.snap b/tests/noltr/main.nf.test.snap index c336d8a5..68b8513b 100644 --- a/tests/noltr/main.nf.test.snap +++ b/tests/noltr/main.nf.test.snap @@ -56,8 +56,6 @@ "stable paths": [ "FI1_stats.csv:md5,8d1274e52117e39b413ff71a76c40331", "sarscov2_stats.csv:md5,9ec4147b73cae550a38337520fc028dd", - "FI1.LAI.out:md5,c958c2f4187e0ae42060d304556e0e6a", - "FI1.LTRlib.fa:md5,e7cc7ee7269dd5a09773cfbedba0de06", "FI1.short.ids.tsv:md5,948c19c1ccd05463a1f5d23f0615b169", "sarscov2.short.ids.tsv:md5,d7a2af88e8549586e5616bff6a88bd71" ], @@ -84,6 +82,6 @@ "nf-test": "0.9.0", "nextflow": "24.04.4" }, - "timestamp": "2024-10-10T15:40:52.594249" + "timestamp": "2024-10-10T20:38:08.028244" } } \ No newline at end of file From a03629a2db5054c9cc6cb8b3f8ee6855bd386840 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 21:36:00 +1300 Subject: [PATCH 33/62] Updated tests --- .github/include.yaml | 2 +- tests/noltr/main.nf.test | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/include.yaml b/.github/include.yaml index a3629f4c..5d850e13 100644 --- a/.github/include.yaml +++ b/.github/include.yaml @@ -1,10 +1,10 @@ ".": - ./.github/workflows/** - ./nf-test.config - - ./nextflow.config tests: - ./assets/* - ./bin/* - ./conf/* - ./main.nf - ./nextflow_schema.json + - ./nextflow.config diff --git a/tests/noltr/main.nf.test b/tests/noltr/main.nf.test index b42eaa68..5d20a1ff 100644 --- a/tests/noltr/main.nf.test +++ b/tests/noltr/main.nf.test @@ -42,7 +42,7 @@ nextflow_pipeline { assertAll( { assert workflow.success}, { - def lai = file("$outputDir/lai/FI1.LAI.out").readLines()[1].split('\t')[6] + def lai = Float.parseFloat(path("$outputDir/lai/FI1.LAI.out").text.split("\n")[1].split("\t")[6]) assert Math.abs(lai - 4.84) <= 1.0 }, { assert snapshot( From 49f9a8a02d0eb32cac4b210ebd7eae39a5dc4858 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 10 Oct 2024 22:02:02 +1300 Subject: [PATCH 34/62] Added text/html as content mime type for the report file --- CHANGELOG.md | 1 + conf/modules.config | 15 ++++++++++++--- 2 files changed, 13 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 749d7926..0ce92b0d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 2. Updated nf-core/template to 3.0.1 [#149](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/149) 3. Updated `samtools faidx` to 1.21 4. Now using nf-test for pipeline level testing [#153](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/153) +5. Added `text/html` as content mime type for the report file [#146](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/146) ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 24722302..817e6f0c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -387,9 +387,18 @@ process { withName: '.*:ASSEMBLYQC:CREATEREPORT' { publishDir = [ - path: { "$params.outdir" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + [ + path: { "$params.outdir" }, + mode: params.publish_dir_mode, + pattern: 'report.json', + contentType: 'application/json' + ], + [ + path: { "$params.outdir" }, + mode: params.publish_dir_mode, + pattern: 'report.html', + contentType: 'text/html' + ] ] } } From f98f3c6c93824ddb79e15166fd857d249efbfc7f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 14 Oct 2024 10:52:11 +1300 Subject: [PATCH 35/62] Version string --- .nf-core.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.nf-core.yml b/.nf-core.yml index a379021b..ec027f90 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -44,5 +44,5 @@ template: - igenomes - multiqc - fastqc - version: 2.2.0+dev + version: 2.2.0dev update: null From fec91354103a7555b7ac4e3d23e9236798e268f1 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 14 Oct 2024 11:02:11 +1300 Subject: [PATCH 36/62] Merge TEMPLATE version 3.0.2 --- .gitignore | 1 + .nf-core.yml | 8 +++---- CHANGELOG.md | 4 ++-- CITATION.cff | 2 +- modules.json | 4 ++-- nextflow.config | 2 +- .../utils_nfcore_assemblyqc_pipeline/main.nf | 9 +++----- .../nf-core/utils_nextflow_pipeline/main.nf | 22 ++++++++++--------- .../nf-core/utils_nfcore_pipeline/main.nf | 2 +- 9 files changed, 27 insertions(+), 27 deletions(-) diff --git a/.gitignore b/.gitignore index 837a2cd1..3950461f 100644 --- a/.gitignore +++ b/.gitignore @@ -14,3 +14,4 @@ testing* # nf-test files .nf-test/ .nf-test.log +null/ diff --git a/.nf-core.yml b/.nf-core.yml index ec027f90..3be93146 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,5 +1,8 @@ bump_version: null lint: + actions_ci: false + multiqc_config: false + template_strings: false files_exist: - conf/igenomes.config - conf/igenomes_ignored.config @@ -18,9 +21,6 @@ lint: - .github/workflows/branch.yml - .github/workflows/linting.yml - docs/README.md - multiqc_config: false - template_strings: false - actions_ci: false nextflow_config: - manifest.name - manifest.homePage @@ -28,7 +28,7 @@ lint: - validation.help.afterText - validation.summary.beforeText - validation.summary.afterText -nf_core_version: 3.0.1 +nf_core_version: 3.0.2 org_path: null repository_type: pipeline template: diff --git a/CHANGELOG.md b/CHANGELOG.md index 0ce92b0d..d2308569 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,12 +3,12 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0+dev - [10-Oct-2024] +## v2.2.0dev - [10-Oct-2024] ### `Added` 1. Added Gfastats [#126](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/126) -2. Updated nf-core/template to 3.0.1 [#149](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/149) +2. Updated nf-core/template to 3.0.2 [#149](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/149) 3. Updated `samtools faidx` to 1.21 4. Now using nf-test for pipeline level testing [#153](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/153) 5. Added `text/html` as content mime type for the report file [#146](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/146) diff --git a/CITATION.cff b/CITATION.cff index 3b342d8c..37a9c841 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -25,7 +25,7 @@ authors: - family-names: "Deng" given-names: "Cecilia" title: "AssemblyQC: A Nextflow pipeline for reproducible reporting of assembly quality" -version: 2.2.0+dev +version: 2.2.0dev date-released: 2024-07-30 url: "https://github.com/Plant-Food-Research-Open/assemblyqc" doi: 10.1093/bioinformatics/btae477 diff --git a/modules.json b/modules.json index f44be8f8..a1a83606 100644 --- a/modules.json +++ b/modules.json @@ -281,12 +281,12 @@ }, "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "f533459a222ac53eb4c6bb7a5f574e4069197cdb", + "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "f533459a222ac53eb4c6bb7a5f574e4069197cdb", + "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { diff --git a/nextflow.config b/nextflow.config index 4adfe987..88134d24 100644 --- a/nextflow.config +++ b/nextflow.config @@ -272,7 +272,7 @@ manifest { description = """A Nextflow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=24.04.2' - version = '2.2.0+dev' + version = '2.2.0dev' doi = 'https://doi.org/10.1093/bioinformatics/btae477' } diff --git a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf index 78ad7033..d9fbc33a 100644 --- a/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_assemblyqc_pipeline/main.nf @@ -49,7 +49,6 @@ workflow PIPELINE_INITIALISATION { workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 ) - // // Validate parameters and generate parameter summary to stdout // @@ -59,7 +58,6 @@ workflow PIPELINE_INITIALISATION { null // schema path: nextflow_schema ) - // // Check config provided to the pipeline // @@ -190,10 +188,9 @@ workflow PIPELINE_COMPLETION { take: email // string: email address email_on_fail // string: email address sent on pipeline failure - plaintext_email // boolean: Send plain-text email instead of HTML - - outdir // path: Path to output directory where results will be published - monochrome_logs // boolean: Disable ANSI colour codes in log output + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output hook_url // string: hook URL for notifications diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 4d7f09c0..0fcbf7b3 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -106,17 +106,19 @@ def checkCondaChannels() { def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order - def channel_priority_violation = false - - required_channels_in_order.eachWithIndex { channel, index -> - if (index < required_channels_in_order.size() - 1) { - channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index + 1])) - } - } + def channel_priority_violation = required_channels_in_order != channels.findAll { ch -> ch in required_channels_in_order } if (channels_missing | channel_priority_violation) { - log.warn( - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " There is a problem with your Conda configuration!\n\n" + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + " Please refer to https://bioconda.github.io/\n" + " The observed channel order is \n" + " ${channels}\n" + " but the following channel order is required:\n" + " ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - ) + log.warn """\ + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + There is a problem with your Conda configuration! + You will need to set-up the conda-forge and bioconda channels correctly. + Please refer to https://bioconda.github.io/ + The observed channel order is + ${channels} + but the following channel order is required: + ${required_channels_in_order} + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + """.stripIndent(true) } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index 805dcc42..5cb7bafe 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -62,7 +62,7 @@ def checkProfileProvided(nextflow_cli_args) { def workflowCitation() { def temp_doi_ref = "" def manifest_doi = workflow.manifest.doi.tokenize(",") - // Using a loop to handle multiple DOIs + // Handling multiple DOIs // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list manifest_doi.each { doi_ref -> From 05b9eedac3310517ace080286d1653833f096e4f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 14 Oct 2024 11:15:52 +1300 Subject: [PATCH 37/62] Updated snapshots --- .nf-core.yml | 1 + tests/hicparam/main.nf.test.snap | 4 ++-- tests/invalid/main.nf.test.snap | 4 ++-- tests/minimal/main.nf.test.snap | 2 +- tests/noltr/main.nf.test.snap | 4 ++-- tests/stub/main.nf.test.snap | 4 ++-- tests/tiny/main.nf.test.snap | 4 ++-- 7 files changed, 12 insertions(+), 11 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 3be93146..902fd78b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -16,6 +16,7 @@ lint: - .github/workflows/awsfulltest.yml files_unchanged: - LICENSE + - .gitignore - .github/CONTRIBUTING.md - .github/PULL_REQUEST_TEMPLATE.md - .github/workflows/branch.yml diff --git a/tests/hicparam/main.nf.test.snap b/tests/hicparam/main.nf.test.snap index 6ca72d89..feebbdbc 100644 --- a/tests/hicparam/main.nf.test.snap +++ b/tests/hicparam/main.nf.test.snap @@ -68,7 +68,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0+dev" + "plant-food-research-open/assemblyqc": "v2.2.0dev" } }, "stable paths": [ @@ -99,4 +99,4 @@ }, "timestamp": "2024-10-10T16:11:00.660108" } -} \ No newline at end of file +} diff --git a/tests/invalid/main.nf.test.snap b/tests/invalid/main.nf.test.snap index bdd1665d..9aa72f46 100644 --- a/tests/invalid/main.nf.test.snap +++ b/tests/invalid/main.nf.test.snap @@ -35,7 +35,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0+dev" + "plant-food-research-open/assemblyqc": "v2.2.0dev" } }, "stable paths": [ @@ -51,4 +51,4 @@ }, "timestamp": "2024-10-10T15:59:12.150838" } -} \ No newline at end of file +} diff --git a/tests/minimal/main.nf.test.snap b/tests/minimal/main.nf.test.snap index 7538396f..44a5d9fc 100644 --- a/tests/minimal/main.nf.test.snap +++ b/tests/minimal/main.nf.test.snap @@ -35,7 +35,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0+dev" + "plant-food-research-open/assemblyqc": "v2.2.0dev" } }, "stable paths": [ diff --git a/tests/noltr/main.nf.test.snap b/tests/noltr/main.nf.test.snap index 68b8513b..75ee9f5f 100644 --- a/tests/noltr/main.nf.test.snap +++ b/tests/noltr/main.nf.test.snap @@ -50,7 +50,7 @@ "seqkit": "v2.8.0" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0+dev" + "plant-food-research-open/assemblyqc": "v2.2.0dev" } }, "stable paths": [ @@ -84,4 +84,4 @@ }, "timestamp": "2024-10-10T20:38:08.028244" } -} \ No newline at end of file +} diff --git a/tests/stub/main.nf.test.snap b/tests/stub/main.nf.test.snap index 257765b7..74b3740e 100644 --- a/tests/stub/main.nf.test.snap +++ b/tests/stub/main.nf.test.snap @@ -230,7 +230,7 @@ "untar": 1.34 }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0+dev" + "plant-food-research-open/assemblyqc": "v2.2.0dev" } }, "stable paths": [ @@ -304,4 +304,4 @@ }, "timestamp": "2024-10-10T14:56:44.730644" } -} \ No newline at end of file +} diff --git a/tests/tiny/main.nf.test.snap b/tests/tiny/main.nf.test.snap index c3a241a3..60dfdbbe 100644 --- a/tests/tiny/main.nf.test.snap +++ b/tests/tiny/main.nf.test.snap @@ -29,7 +29,7 @@ "pigz": "2.3.4" }, "Workflow": { - "plant-food-research-open/assemblyqc": "v2.2.0+dev" + "plant-food-research-open/assemblyqc": "v2.2.0dev" } }, "stable paths": [ @@ -44,4 +44,4 @@ }, "timestamp": "2024-10-10T14:46:59.600343" } -} \ No newline at end of file +} From af9a257d88d488b8f1b35c809e8e57f31aab8a7a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 15 Oct 2024 11:37:53 +1300 Subject: [PATCH 38/62] Added a sequence labels table below the HiC contact map --- CHANGELOG.md | 5 ++-- bin/report_modules/parsers/hic_parser.py | 18 +++++++++++++++ .../templates/hic/report_contents.html | 23 ++++++++++--------- subworkflows/local/fq2hic.nf | 1 + workflows/assemblyqc.nf | 7 +++++- 5 files changed, 40 insertions(+), 14 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d2308569..7a652177 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0dev - [10-Oct-2024] +## v2.2.0dev - [15-Oct-2024] ### `Added` @@ -12,6 +12,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 3. Updated `samtools faidx` to 1.21 4. Now using nf-test for pipeline level testing [#153](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/153) 5. Added `text/html` as content mime type for the report file [#146](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/146) +6. Added a sequence labels table below the HiC contact map [#147](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/147) ### `Fixed` @@ -19,7 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Dependencies` -1. Nextflow!>=23.04.0 +1. Nextflow!>=24.04.2 2. nf-schema@2.1.1 ### `Deprecated` diff --git a/bin/report_modules/parsers/hic_parser.py b/bin/report_modules/parsers/hic_parser.py index 825852ec..01992ecd 100644 --- a/bin/report_modules/parsers/hic_parser.py +++ b/bin/report_modules/parsers/hic_parser.py @@ -1,5 +1,7 @@ import os from pathlib import Path +import pandas as pd +from tabulate import tabulate import re from report_modules.parsers.parsing_commons import sort_list_of_results @@ -24,10 +26,26 @@ def parse_hic_folder(folder_name="hic_outputs"): hic_file_name, )[0] + labels_table = pd.read_csv(f"{folder_name}/{file_tokens}.agp.assembly", sep=" ") + + labels_table = labels_table[labels_table.iloc[:, 0].str.startswith(">")].iloc[ + :, [0, 2] + ] + labels_table.columns = ["Sequence", "Length"] + labels_table.Length = labels_table.Length.astype(int) + data["HIC"].append( { "hap": file_tokens, "hic_html_file_name": hic_file_name, + "labels_table": labels_table.to_dict("records"), + "labels_table_html": tabulate( + labels_table, + headers=["Sequence", "Length"], + tablefmt="html", + numalign="left", + showindex=False, + ), } ) diff --git a/bin/report_modules/templates/hic/report_contents.html b/bin/report_modules/templates/hic/report_contents.html index 312a0fdd..4a7f3089 100644 --- a/bin/report_modules/templates/hic/report_contents.html +++ b/bin/report_modules/templates/hic/report_contents.html @@ -1,17 +1,18 @@ {% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["HIC"]|length) %} {% set active_text = 'display: block' if vars.is_first else 'display: none' %}
    -
    -
    -
    {{ all_stats_dicts['HIC'][item]['hap'] }}
    -
    -
    - -
    +
    +
    +
    {{ all_stats_dicts['HIC'][item]['hap'] }}
    +
    + +
    +
    +
    +
    +
    {{ all_stats_dicts['HIC'][item]['labels_table_html'] }}
    +
    +
    {% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/subworkflows/local/fq2hic.nf b/subworkflows/local/fq2hic.nf index 34a7fa4e..a37a325a 100644 --- a/subworkflows/local/fq2hic.nf +++ b/subworkflows/local/fq2hic.nf @@ -97,5 +97,6 @@ workflow FQ2HIC { emit: hic = ch_hic html = HIC2HTML.out.html + assembly = AGP2ASSEMBLY.out.assembly versions = ch_versions } diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 4dd9067b..c892263c 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -591,6 +591,11 @@ workflow ASSEMBLYQC { ) ch_hic_html = FQ2HIC.out.html + ch_hic_assembly = FQ2HIC.out.assembly + ch_hic_report_files = ch_hic_html + | mix( + ch_hic_assembly.map { tag, assembly -> assembly } + ) ch_versions = ch_versions.mix(FQ2HIC.out.versions) // SUBWORKFLOW: FASTA_SYNTENY @@ -822,7 +827,7 @@ workflow ASSEMBLYQC { ch_tidk_outputs .collect().ifEmpty([]), ch_lai_outputs .collect().ifEmpty([]), ch_kraken2_plot .collect().ifEmpty([]), - ch_hic_html .collect().ifEmpty([]), + ch_hic_report_files .collect().ifEmpty([]), ch_synteny_outputs .collect().ifEmpty([]), ch_merqury_outputs .collect().ifEmpty([]), ch_versions_yml, From cbbee9d2885ab3aa9acad84daf2548038b6fa8ad Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 15 Oct 2024 12:36:28 +1300 Subject: [PATCH 39/62] Increased memory requirement for DNADIFF to avoid SLURM OOM kills with exit code 2 --- CHANGELOG.md | 1 + conf/base.config | 1 + 2 files changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7a652177..42bd00e1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Fixed` 1. Fixed a bug where Gene score distribution graph did not appear correctly [#125](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/125) +2. Increased memory requirement for `DNADIFF` to avoid SLURM OOM kills with exit code 2 [#141](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/141) ### `Dependencies` diff --git a/conf/base.config b/conf/base.config index 20473408..f13b4759 100644 --- a/conf/base.config +++ b/conf/base.config @@ -71,6 +71,7 @@ process { } withName:DNADIFF { time = { 7.day * task.attempt } + memory = { 12.GB * task.attempt } } withName:MERQURY_HAPMERS { time = { 20.h * task.attempt } From 27a8d3b4d3081a987615f0b75464c385466e3147 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 25 Oct 2024 15:09:31 +1300 Subject: [PATCH 40/62] Added explicit revision param to docs --- README.md | 1 + conf/test.config | 2 +- conf/test_full.config | 2 +- docs/usage.md | 4 ++-- 4 files changed, 5 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 4737e2e8..8d9b478e 100644 --- a/README.md +++ b/README.md @@ -102,6 +102,7 @@ Now, you can run the pipeline using: ```bash nextflow run plant-food-research-open/assemblyqc \ + -revision \ -profile \ --input assemblysheet.csv \ --outdir diff --git a/conf/test.config b/conf/test.config index 2417719d..e86178f3 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run plant-food-research-open/assemblyqc -profile test, --outdir + nextflow run plant-food-research-open/assemblyqc -revision -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_full.config b/conf/test_full.config index 2eadbf9b..818e1c86 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run plant-food-research-open/assemblyqc -profile test_full, --outdir + nextflow run plant-food-research-open/assemblyqc -revision -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/usage.md b/docs/usage.md index 20f10823..3e8b314b 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -136,7 +136,7 @@ All the modules have been tested to work on a single machine with 10 CPUs + 32 G The typical command for running the pipeline is as follows: ```bash -nextflow run plant-food-research-open/assemblyqc --input ./assemblysheet.csv --outdir ./results -profile docker +nextflow run plant-food-research-open/assemblyqc -revision --input ./assemblysheet.csv --outdir ./results -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -160,7 +160,7 @@ Pipeline settings can be provided in a `yaml` or `json` file via `-params-file < The above pipeline run specified with a params file in yaml format: ```bash -nextflow run plant-food-research-open/assemblyqc -profile docker -params-file params.yaml +nextflow run plant-food-research-open/assemblyqc -revision main -profile docker -params-file params.yaml ``` with: From 62e19f584b769e9cd48763bd962a69005acd8248 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 25 Oct 2024 15:12:21 +1300 Subject: [PATCH 41/62] Updated changelog --- CHANGELOG.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 42bd00e1..b53c1203 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0dev - [15-Oct-2024] +## v2.2.0dev - [25-Oct-2024] ### `Added` @@ -18,6 +18,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 1. Fixed a bug where Gene score distribution graph did not appear correctly [#125](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/125) 2. Increased memory requirement for `DNADIFF` to avoid SLURM OOM kills with exit code 2 [#141](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/141) +3. Documented the use explicit use of `-revision` parameter [#160](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/160) ### `Dependencies` From c1f83cc16c09a7f14b34b1b145a3b1437e8775d8 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 25 Oct 2024 16:27:13 +1300 Subject: [PATCH 42/62] Added parameter hic_samtools_ext_args --- CHANGELOG.md | 1 + conf/modules.config | 5 ++++- docs/parameters.md | 13 +++++++------ nextflow.config | 1 + nextflow_schema.json | 6 ++++++ tests/README.md | 6 +++++- 6 files changed, 24 insertions(+), 8 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index b53c1203..78f86c63 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 4. Now using nf-test for pipeline level testing [#153](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/153) 5. Added `text/html` as content mime type for the report file [#146](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/146) 6. Added a sequence labels table below the HiC contact map [#147](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/147) +7. Added parameter `hic_samtools_ext_args` and set its default value to `-F 3852` [#159](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/159) ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 817e6f0c..0f05443c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -328,7 +328,10 @@ process { withName: '.*:FQ2HIC:FASTQ_BWA_MEM_SAMBLASTER:SAMBLASTER' { ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } - ext.args3 = '-h -F 2316' + ext.args3 = [ + '-h', + params.hic_samtools_ext_args ? params.hic_samtools_ext_args.split("\\s(?=-+)") : '' + ].flatten().unique(false).join(' ').trim() } withName: '.*:FQ2HIC:AGP2ASSEMBLY' { diff --git a/docs/parameters.md b/docs/parameters.md index a6b2e912..90a39759 100644 --- a/docs/parameters.md +++ b/docs/parameters.md @@ -67,12 +67,13 @@ A Nextflow pipeline which evaluates assembly quality with multiple QC tools and ## HiC options -| Parameter | Description | Type | Default | Required | Hidden | -| -------------------- | ---------------------------------------------------------------------------------------- | --------- | ------------------------------------------------- | -------- | ------ | -| `hic` | HiC reads path provided as a SRA ID or as paired reads such as 'hic_reads{1,2}.fastq.gz' | `string` | | | | -| `hic_skip_fastp` | Skip HiC read trimming | `boolean` | | | | -| `hic_skip_fastqc` | Skip HiC read QC | `boolean` | | | | -| `hic_fastp_ext_args` | Additional parameters for fastp trimming | `string` | --qualified_quality_phred 20 --length_required 50 | | | +| Parameter | Description | Type | Default | Required | Hidden | +| ----------------------- | ---------------------------------------------------------------------------------------- | --------- | ------------------------------------------------- | -------- | ------ | +| `hic` | HiC reads path provided as a SRA ID or as paired reads such as 'hic_reads{1,2}.fastq.gz' | `string` | | | | +| `hic_skip_fastp` | Skip HiC read trimming | `boolean` | | | | +| `hic_skip_fastqc` | Skip HiC read QC | `boolean` | | | | +| `hic_fastp_ext_args` | Additional parameters for fastp trimming | `string` | --qualified_quality_phred 20 --length_required 50 | | | +| `hic_samtools_ext_args` | Additional parameters for samtools view command run after samblaster | `string` | -F 3852 | | | ## Synteny options diff --git a/nextflow.config b/nextflow.config index 88134d24..91e2ddf2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -53,6 +53,7 @@ params { hic_skip_fastp = false hic_skip_fastqc = false hic_fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' + hic_samtools_ext_args = '-F 3852' // Synteny options synteny_skip = true diff --git a/nextflow_schema.json b/nextflow_schema.json index eda14c7a..6b5b9d63 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -237,6 +237,12 @@ "default": "--qualified_quality_phred 20 --length_required 50", "description": "Additional parameters for fastp trimming", "fa_icon": "fas fa-terminal" + }, + "hic_samtools_ext_args": { + "type": "string", + "default": "-F 3852", + "fa_icon": "fas fa-terminal", + "description": "Additional parameters for samtools view command run after samblaster" } } }, diff --git a/tests/README.md b/tests/README.md index 6e935db0..6f09f846 100644 --- a/tests/README.md +++ b/tests/README.md @@ -16,7 +16,11 @@ nextflow run plant-food-research-open/assemblyqc -r main -profile singularity,te ## nf-test and Continuous Integration (CI) -The GitHub [CI action](../.github/workflows/ci.yml) included with the pipeline continuously tests the pipeline using [nf-test](https://www.nf-test.com). +The GitHub [CI action](../.github/workflows/ci.yml) included with the pipeline continuously tests the pipeline using [nf-test](https://www.nf-test.com). Many components included with the pipeline such as [minimap2/align](../modules/nf-core/minimap2/align) include their own [tests](../modules/nf-core/minimap2/align/tests/main.nf.test) with test data from nf-core. + +## Testing with a Large Dataset at Plant&Food + +Before each release, the functionality of the entire pipeline is tested with a large dataset on the on-prem SLURM-based HPC at The New Zealand Institute of Plant and Food Research. ## Testing Merqury Datasets From 5c769fa85b3c6266d1cabfdd7d671eba11cca12d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 31 Oct 2024 16:22:30 +1300 Subject: [PATCH 43/62] Added the HiC QC report to the final report --- CHANGELOG.md | 3 ++- bin/report_modules/parsers/hic_parser.py | 19 +++++++++++++++---- bin/report_modules/templates/header.html | 12 ++++++++++++ .../templates/hic/report_contents.html | 13 +++++++++++-- subworkflows/local/fq2hic.nf | 2 ++ workflows/assemblyqc.nf | 4 ++++ 6 files changed, 46 insertions(+), 7 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 78f86c63..0be19d33 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0dev - [25-Oct-2024] +## v2.2.0dev - [31-Oct-2024] ### `Added` @@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 5. Added `text/html` as content mime type for the report file [#146](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/146) 6. Added a sequence labels table below the HiC contact map [#147](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/147) 7. Added parameter `hic_samtools_ext_args` and set its default value to `-F 3852` [#159](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/159) +8. Added the HiC QC report to the final report so that users don't have to navigate to the results folder [#162](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/162) ### `Fixed` diff --git a/bin/report_modules/parsers/hic_parser.py b/bin/report_modules/parsers/hic_parser.py index 01992ecd..17231e17 100644 --- a/bin/report_modules/parsers/hic_parser.py +++ b/bin/report_modules/parsers/hic_parser.py @@ -15,28 +15,38 @@ def parse_hic_folder(folder_name="hic_outputs"): return {} list_of_hic_files = hic_folder_path.glob("*.html") + list_of_hic_files = [ + x for x in list_of_hic_files if re.match(r"^\w+\.html$", x.name) + ] data = {"HIC": []} for hic_path in list_of_hic_files: hic_file_name = os.path.basename(str(hic_path)) - file_tokens = re.findall( + tag = re.findall( r"([\w]+).html", hic_file_name, )[0] - labels_table = pd.read_csv(f"{folder_name}/{file_tokens}.agp.assembly", sep=" ") - + # Get the labels table + labels_table = pd.read_csv(f"{folder_name}/{tag}.agp.assembly", sep=" ") labels_table = labels_table[labels_table.iloc[:, 0].str.startswith(">")].iloc[ :, [0, 2] ] labels_table.columns = ["Sequence", "Length"] labels_table.Length = labels_table.Length.astype(int) + # Get the HiC QC report + hicqc_report = [ + x + for x in hic_folder_path.glob("*.pdf") + if re.match(rf"[\S]+\.on\.{tag}_qc_report\.pdf", x.name) + ][0] + data["HIC"].append( { - "hap": file_tokens, + "hap": tag, "hic_html_file_name": hic_file_name, "labels_table": labels_table.to_dict("records"), "labels_table_html": tabulate( @@ -46,6 +56,7 @@ def parse_hic_folder(folder_name="hic_outputs"): numalign="left", showindex=False, ), + "hicqc_report_pdf": os.path.basename(str(hicqc_report)), } ) diff --git a/bin/report_modules/templates/header.html b/bin/report_modules/templates/header.html index 989b37f0..795ecd3d 100644 --- a/bin/report_modules/templates/header.html +++ b/bin/report_modules/templates/header.html @@ -213,6 +213,18 @@ .iframe-wrapper { text-align: center; + width: 90%; + margin-left: auto; + margin-right: auto; + margin-bottom: 32px; + } + + .iframe-wrapper-hic { + width: 700px; + height: 850px; + margin-left: auto; + margin-right: auto; + margin-bottom: 32px; } .tab { diff --git a/bin/report_modules/templates/hic/report_contents.html b/bin/report_modules/templates/hic/report_contents.html index 4a7f3089..b35fa5dd 100644 --- a/bin/report_modules/templates/hic/report_contents.html +++ b/bin/report_modules/templates/hic/report_contents.html @@ -5,14 +5,23 @@
    {{ all_stats_dicts['HIC'][item]['hap'] }}
    -
    - +
    +
    +
    +

    Sequence labels and lengths

    +
    {{ all_stats_dicts['HIC'][item]['labels_table_html'] }}
    +
    +

    HiC QC report

    +
    +
    + +
    {% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/subworkflows/local/fq2hic.nf b/subworkflows/local/fq2hic.nf index a37a325a..7b00cae2 100644 --- a/subworkflows/local/fq2hic.nf +++ b/subworkflows/local/fq2hic.nf @@ -64,6 +64,7 @@ workflow FQ2HIC { HICQC ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3, bam ] } ) + ch_hicqc_pdf = HICQC.out.pdf ch_versions = ch_versions.mix(HICQC.out.versions) // MODULE: MAKEAGPFROMFASTA | AGP2ASSEMBLY | ASSEMBLY2BEDPE @@ -95,6 +96,7 @@ workflow FQ2HIC { ch_versions = ch_versions.mix(HIC2HTML.out.versions.first()) emit: + hicqc_pdf = ch_hicqc_pdf hic = ch_hic html = HIC2HTML.out.html assembly = AGP2ASSEMBLY.out.assembly diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index c892263c..103e1618 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -590,12 +590,16 @@ workflow ASSEMBLYQC { params.hic_skip_fastqc ) + ch_hicqc_pdf = FQ2HIC.out.hicqc_pdf ch_hic_html = FQ2HIC.out.html 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z-hIP;!|(YY?pRI)oB>1j*X*x?zXfl9UIA#}NFsco%EOByRy3R$Gydm;12@BSlp2JG zm7Hzx+Wh5rf8MKqx%;RR;EY5b>i-|V{+CySzxr!|@zk+Zw({+Nz64ZYbo~Dx@o$IS iKmCyW|HFm4!dlt&`GP3n0)qqpNxYDKUieJc>;D1reb+Pq literal 0 HcmV?d00001 diff --git a/docs/output.md b/docs/output.md index f5bfacff..f124df2d 100644 --- a/docs/output.md +++ b/docs/output.md @@ -198,7 +198,12 @@ Kraken2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to seq Hi-C contact mapping experiments measure the frequency of physical contact between loci in the genome. The resulting dataset, called a “contact map,” is represented using a [two-dimensional heatmap](https://github.com/igvteam/juicebox.js) where the intensity of each pixel indicates the frequency of contact between a pair of loci. -
    AssemblyQC - HiC interactive contact map
    AssemblyQC - HiC interactive contact map
    +
    +AssemblyQC - HiC QC report +AssemblyQC - HiC interactive contact map +
    +AssemblyQC - HiC results +
    ### Synteny From d4624de8337451d8c31c292c29b19713158ccc85 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 31 Oct 2024 21:55:09 +1300 Subject: [PATCH 45/62] Added the fastp log to the final report --- CHANGELOG.md | 1 + bin/report_modules/parsers/hic_parser.py | 54 ++++++++++++++++ bin/report_modules/templates/hic/hic.html | 59 +++++++++++++----- .../templates/hic/report_contents.html | 10 ++- docs/images/fastp.png | Bin 0 -> 209262 bytes docs/output.md | 5 +- subworkflows/local/fq2hic.nf | 6 +- workflows/assemblyqc.nf | 4 ++ 8 files changed, 117 insertions(+), 22 deletions(-) create mode 100644 docs/images/fastp.png diff --git a/CHANGELOG.md b/CHANGELOG.md index 0be19d33..fb8159fa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 6. Added a sequence labels table below the HiC contact map [#147](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/147) 7. Added parameter `hic_samtools_ext_args` and set its default value to `-F 3852` [#159](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/159) 8. Added the HiC QC report to the final report so that users don't have to navigate to the results folder [#162](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/162) +9. Added the fastp log to the final report [#163](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/163) ### `Fixed` diff --git a/bin/report_modules/parsers/hic_parser.py b/bin/report_modules/parsers/hic_parser.py index 17231e17..45d52fbf 100644 --- a/bin/report_modules/parsers/hic_parser.py +++ b/bin/report_modules/parsers/hic_parser.py @@ -7,6 +7,50 @@ from report_modules.parsers.parsing_commons import sort_list_of_results +def colorize_fastp_log(log: Path): + section_colors = { + "adapter": "color: blue;", + "before_filtering": "color: goldenrod;", + "after_filtering": "color: green;", + "filtering_result": "color: green;", + "duplication": "color: red;", + "fastp": "color: gray;", + "version": "color: blue;", + } + + patterns = { + "adapter": re.compile(r"Detecting adapter sequence for read\d..."), + "before_filtering": re.compile(r"Read\d before filtering:"), + "after_filtering": re.compile(r"Read\d after filtering:"), + "filtering_result": re.compile(r"Filtering result:"), + "duplication": re.compile(r"Duplication rate:"), + "fastp": re.compile(r"fastp --in"), + "version": re.compile(r"fastp v"), + } + + html_log = "
    \n"
    +
    +    for line in log.read_text().split("\n"):
    +        colored_line = line.strip()
    +        # Apply HTML color style based on section patterns
    +        for section, pattern in patterns.items():
    +            if pattern.search(line):
    +                colored_line = (
    +                    f"{line.strip()}"
    +                )
    +                break
    +        else:
    +            # Default styling for uncolored lines
    +            colored_line = f"{line.strip()}"
    +
    +        html_log += f"{colored_line}\n"
    +
    +    # Close HTML tags
    +    html_log += "
    " + + return html_log + + def parse_hic_folder(folder_name="hic_outputs"): dir = os.getcwdb().decode() hic_folder_path = Path(f"{dir}/{folder_name}") @@ -44,6 +88,15 @@ def parse_hic_folder(folder_name="hic_outputs"): if re.match(rf"[\S]+\.on\.{tag}_qc_report\.pdf", x.name) ][0] + # Get FASTP log if it is there + fastp_log = [x for x in hic_folder_path.glob("*.log")] + + if fastp_log != []: + fastp_log = fastp_log[0] + fastp_log = colorize_fastp_log(fastp_log) + else: + fastp_log = None + data["HIC"].append( { "hap": tag, @@ -57,6 +110,7 @@ def parse_hic_folder(folder_name="hic_outputs"): showindex=False, ), "hicqc_report_pdf": os.path.basename(str(hicqc_report)), + "fastp_log": fastp_log, } ) diff --git a/bin/report_modules/templates/hic/hic.html b/bin/report_modules/templates/hic/hic.html index 868dc089..2af561e7 100644 --- a/bin/report_modules/templates/hic/hic.html +++ b/bin/report_modules/templates/hic/hic.html @@ -1,18 +1,45 @@
    diff --git a/bin/report_modules/templates/hic/report_contents.html b/bin/report_modules/templates/hic/report_contents.html index b35fa5dd..c7f19be8 100644 --- a/bin/report_modules/templates/hic/report_contents.html +++ b/bin/report_modules/templates/hic/report_contents.html @@ -8,8 +8,6 @@
    - -

    Sequence labels and lengths

    @@ -22,6 +20,14 @@
    + {% if all_stats_dicts['HIC'][item]['fastp_log'] is not none %} +
    +

    fastp log

    +
    +
    + {{ all_stats_dicts['HIC'][item]['fastp_log'] }} +
    + {% endif %}
    {% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/docs/images/fastp.png b/docs/images/fastp.png new file mode 100644 index 0000000000000000000000000000000000000000..b968889ae6c6b7d7cdd6e2e629133424ff3b6fec GIT binary patch literal 209262 zcmbTdbzD@>`v*!%htf!k0)nt~ryvMOcS}o4gT#uIQj3IiNOz~i(jC$z-QB(H-s89Q zbAR`*yRWl*&dxb=W}auBnWx_KMMXBo7T5)u-g+-qqyBqS6#5)#@bCOS}}%aP-T zgoLYPEhVKQCnZIv;_PT?ZD)am^!iJjHinLRA8DGt8a*1Ow1oV&;=`|m67raA=aF=-bS9BaFnS8Co-l>d4S9Eq$`38ySmTrXQq4`T{p>R?Gxj<8_PQQnD z>l;H4Up8TyQocjo%4WTo-7(%VhbwaXO2>?hP_-56pyLQiyoldC-GZg>TrWKXX>*E% z@D}gtRz~Sq401RsJ6i@muMp~Ve75sd^`R~5$5qpnjAlaaDq?yp7Vilex~=vUWX8^z z^#-RVdNHkD0?dG!hpV`94(8WUimJf^udPwfLbN`gd7V7mlQdw6#^}bIO^F`*Or3UR z85}-9vD$c3DM=f9n@%E#MHMHOoBC`OZ^tY$NcTkUp*lT2^Ft z9AT7#b`0vBEM^rSbr_@3?uyPUPH}Zr_M`3(MQ+~j&lJ4i1UX|G4~>KpsBU0;KUpTY zB`|Bdl_pebFOPgJCg>wT`kk3hg+y41RE}^}L!^{x=nA44(RGmBFe-If4`t@p=}VTR z0)akL(&$GKkmbqKg1&9a_7uT7S*vcokFtP|@9C)COCr5Reo(yg7QGjJ^>VS>$TM#j 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    AssemblyQC - HiC results diff --git a/subworkflows/local/fq2hic.nf b/subworkflows/local/fq2hic.nf index 7b00cae2..22eed2ef 100644 --- a/subworkflows/local/fq2hic.nf +++ b/subworkflows/local/fq2hic.nf @@ -34,6 +34,7 @@ workflow FQ2HIC { 1 // min_trimmed_reads ) + ch_fastp_log = FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_log ch_trim_reads = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions) @@ -64,7 +65,7 @@ workflow FQ2HIC { HICQC ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3, bam ] } ) - ch_hicqc_pdf = HICQC.out.pdf + ch_hicqc_pdf = HICQC.out.pdf ch_versions = ch_versions.mix(HICQC.out.versions) // MODULE: MAKEAGPFROMFASTA | AGP2ASSEMBLY | ASSEMBLY2BEDPE @@ -96,7 +97,8 @@ workflow FQ2HIC { ch_versions = ch_versions.mix(HIC2HTML.out.versions.first()) emit: - hicqc_pdf = ch_hicqc_pdf + fastp_log = ch_fastp_log + hicqc_pdf = ch_hicqc_pdf hic = ch_hic html = HIC2HTML.out.html assembly = AGP2ASSEMBLY.out.assembly diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 103e1618..9116a5ea 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -590,6 +590,7 @@ workflow ASSEMBLYQC { params.hic_skip_fastqc ) + ch_hic_fastp_log = FQ2HIC.out.fastp_log ch_hicqc_pdf = FQ2HIC.out.hicqc_pdf ch_hic_html = FQ2HIC.out.html ch_hic_assembly = FQ2HIC.out.assembly @@ -600,6 +601,9 @@ workflow ASSEMBLYQC { | mix( ch_hicqc_pdf.map { meta, pdf -> pdf } ) + | mix( + ch_hic_fastp_log.map { meta, log -> log } + ) ch_versions = ch_versions.mix(FQ2HIC.out.versions) // SUBWORKFLOW: FASTA_SYNTENY From 2e8844d3d8c865ffee80495fe1d50cbd4709916a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Sun, 3 Nov 2024 01:06:56 +1300 Subject: [PATCH 46/62] Added orthofinder --- README.md | 74 +---- bin/assemblyqc.py | 2 + .../parsers/orthofinder_parser.py | 100 +++++++ bin/report_modules/templates/base.html | 10 + .../templates/orthofinder/orthofinder.html | 14 + .../orthofinder/report_contents.html | 33 +++ conf/modules.config | 12 + docs/images/assemblyqc.png | Bin 0 -> 547780 bytes docs/parameters.md | 6 + modules.json | 10 + modules/local/createreport.nf | 1 + modules/nf-core/gffread/environment.yml | 5 + modules/nf-core/gffread/main.nf | 60 ++++ modules/nf-core/gffread/meta.yml | 75 +++++ modules/nf-core/gffread/tests/main.nf.test | 223 ++++++++++++++ .../nf-core/gffread/tests/main.nf.test.snap | 272 ++++++++++++++++++ .../gffread/tests/nextflow-fasta.config | 5 + .../gffread/tests/nextflow-gff3.config | 5 + modules/nf-core/gffread/tests/nextflow.config | 5 + modules/nf-core/gffread/tests/tags.yml | 2 + modules/nf-core/orthofinder/environment.yml | 6 + modules/nf-core/orthofinder/main.nf | 80 ++++++ modules/nf-core/orthofinder/meta.yml | 71 +++++ .../nf-core/orthofinder/tests/main.nf.test | 161 +++++++++++ .../orthofinder/tests/main.nf.test.snap | 171 +++++++++++ modules/nf-core/orthofinder/tests/tags.yml | 2 + nextflow.config | 3 + nextflow_schema.json | 17 ++ tests/orthofinder/assemblysheet.csv | 5 + tests/orthofinder/main.nf.test | 36 +++ tests/orthofinder/main.nf.test.snap | 83 ++++++ workflows/assemblyqc.nf | 35 +++ 32 files changed, 1521 insertions(+), 63 deletions(-) create mode 100644 bin/report_modules/parsers/orthofinder_parser.py create mode 100644 bin/report_modules/templates/orthofinder/orthofinder.html create mode 100644 bin/report_modules/templates/orthofinder/report_contents.html create mode 100644 docs/images/assemblyqc.png create mode 100644 modules/nf-core/gffread/environment.yml create mode 100644 modules/nf-core/gffread/main.nf create mode 100644 modules/nf-core/gffread/meta.yml create mode 100644 modules/nf-core/gffread/tests/main.nf.test create mode 100644 modules/nf-core/gffread/tests/main.nf.test.snap create mode 100644 modules/nf-core/gffread/tests/nextflow-fasta.config create mode 100644 modules/nf-core/gffread/tests/nextflow-gff3.config create mode 100644 modules/nf-core/gffread/tests/nextflow.config create mode 100644 modules/nf-core/gffread/tests/tags.yml create mode 100644 modules/nf-core/orthofinder/environment.yml create mode 100644 modules/nf-core/orthofinder/main.nf create mode 100644 modules/nf-core/orthofinder/meta.yml create mode 100644 modules/nf-core/orthofinder/tests/main.nf.test create mode 100644 modules/nf-core/orthofinder/tests/main.nf.test.snap create mode 100644 modules/nf-core/orthofinder/tests/tags.yml create mode 100644 tests/orthofinder/assemblysheet.csv create mode 100644 tests/orthofinder/main.nf.test create mode 100644 tests/orthofinder/main.nf.test.snap diff --git a/README.md b/README.md index 8d9b478e..2283fc0b 100644 --- a/README.md +++ b/README.md @@ -16,61 +16,7 @@ ## Pipeline Flowchart -```mermaid -%%{init: { - 'theme': 'base', - 'themeVariables': { - 'fontSize': '52px", - 'primaryColor': '#9A6421', - 'primaryTextColor': '#ffffff', - 'primaryBorderColor': '#9A6421', - 'lineColor': '#B180A8', - 'secondaryColor': '#455C58', - 'tertiaryColor': '#ffffff' - } -}}%% -flowchart LR - forEachTag(Assembly) ==> VALIDATE_FORMAT[VALIDATE FORMAT] - - VALIDATE_FORMAT ==> ncbiFCS[NCBI FCS ADAPTOR] - ncbiFCS ==> Check{Check} - - VALIDATE_FORMAT ==> ncbiGX[NCBI FCS GX] - ncbiGX ==> Check - Check ==> |Clean|Run(Run) - - Check ==> |Contamination|Skip(Skip All) - Skip ==> REPORT - - VALIDATE_FORMAT ==> GFF_STATS[GENOMETOOLS GT STAT] - - Run ==> ASS_STATS[STATS] - Run ==> BUSCO - Run ==> TIDK - Run ==> LAI - Run ==> KRAKEN2 - Run ==> HIC_CONTACT_MAP[HIC CONTACT MAP] - Run ==> MUMMER - Run ==> MINIMAP2 - Run ==> MERQURY - - MUMMER ==> CIRCOS - MUMMER ==> DOTPLOT - - MINIMAP2 ==> PLOTSR - - ASS_STATS ==> REPORT - GFF_STATS ==> REPORT - BUSCO ==> REPORT - TIDK ==> REPORT - LAI ==> REPORT - KRAKEN2 ==> REPORT - HIC_CONTACT_MAP ==> REPORT - CIRCOS ==> REPORT - DOTPLOT ==> REPORT - PLOTSR ==> REPORT - MERQURY ==> REPORT -``` +

    - [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator) + [SEQKIT RMDUP](https://github.com/shenwei356/seqkit): FASTA validation - [GENOMETOOLS GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation @@ -85,6 +31,7 @@ flowchart LR - [HIC CONTACT MAP](https://github.com/igvteam/juicebox.js): Alignment and visualisation of HiC data - [MUMMER](https://github.com/mummer4/mummer) → [CIRCOS](http://circos.ca/documentation/) + [DOTPLOT](https://plotly.com) & [MINIMAP2](https://github.com/lh3/minimap2) → [PLOTSR](https://github.com/schneebergerlab/plotsr): Synteny analysis - [MERQURY](https://github.com/marbl/merqury): K-mer completeness, consensus quality and phasing assessment +- [ORTHOFINDER](https://github.com/davidemms/OrthoFinder): Phylogenetic orthology inference for comparative genomics ## Usage @@ -140,31 +87,32 @@ The pipeline uses nf-core modules contributed by following authors:
    - - + + + + - + + - - + + + - - - diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py index c81db840..c808e4de 100755 --- a/bin/assemblyqc.py +++ b/bin/assemblyqc.py @@ -29,6 +29,7 @@ from report_modules.parsers.hic_parser import parse_hic_folder from report_modules.parsers.synteny_parser import parse_synteny_folder from report_modules.parsers.merqury_parser import parse_merqury_folder +from report_modules.parsers.orthofinder_parser import parse_orthofinder_folder if __name__ == "__main__": params_dict, params_table = parse_params_json("params_json.json") @@ -57,6 +58,7 @@ data_from_tools = {**data_from_tools, **parse_hic_folder()} data_from_tools = {**data_from_tools, **parse_synteny_folder()} data_from_tools = {**data_from_tools, **parse_merqury_folder()} + data_from_tools = {**data_from_tools, **parse_orthofinder_folder()} with open("software_versions.yml", "r") as f: versions_from_ch_versions = yaml.safe_load(f) diff --git a/bin/report_modules/parsers/orthofinder_parser.py b/bin/report_modules/parsers/orthofinder_parser.py new file mode 100644 index 00000000..d31fdfe8 --- /dev/null +++ b/bin/report_modules/parsers/orthofinder_parser.py @@ -0,0 +1,100 @@ +import pandas as pd +import base64 +import os +import re + +import matplotlib.pyplot as plt +from tabulate import tabulate +from pathlib import Path +from io import StringIO +from Bio import Phylo + + +def parse_orthofinder_folder(folder_name="orthofinder_outputs/assemblyqc"): + dir = os.getcwdb().decode() + results_root_path = Path(f"{dir}/{folder_name}") + + if not results_root_path.exists(): + return {} + + data = {"ORTHOFINDER": {}} + + # Species tree + tree = Phylo.read( + f"{results_root_path}/Species_Tree/SpeciesTree_rooted.txt", "newick" + ) + + fig = plt.figure(figsize=(6, 6)) + ax = fig.add_subplot(1, 1, 1) + Phylo.draw(tree, do_show=False, axes=ax) + + plt.gca().spines["top"].set_visible(False) + plt.gca().spines["right"].set_visible(False) + + plt.savefig("speciestree_rooted.png", format="png", dpi=300) + + with open("speciestree_rooted.png", "rb") as f: + binary_fc = f.read() + + base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") + data["ORTHOFINDER"]["speciestree_rooted"] = ( + f"data:image/png+xml;base64,{base64_utf8_str}" + ) + + # Overall statistics + overall_statistics = Path( + f"{results_root_path}/Comparative_Genomics_Statistics/Statistics_Overall.tsv" + ).read_text() + + ## General stats + general_stats = re.findall( + r"(Number of species.*)Orthogroups file", overall_statistics, flags=re.DOTALL + )[0] + general_stats_pd = pd.read_csv(StringIO(general_stats), sep="\t") + + data["ORTHOFINDER"]["general_stats"] = general_stats_pd.to_dict("records") + data["ORTHOFINDER"]["general_stats_html"] = tabulate( + general_stats_pd, + headers=["Stat", "Value"], + tablefmt="html", + numalign="left", + showindex=False, + ) + + ## Genes per-species + genes_per_species = re.findall( + r"(Average number of genes per-species in orthogroup.*)Number of species in orthogroup", + overall_statistics, + flags=re.DOTALL, + )[0] + genes_per_species_pd = pd.read_csv(StringIO(genes_per_species), sep="\t", header=0) + data["ORTHOFINDER"]["genes_per_species"] = genes_per_species_pd.to_dict("records") + data["ORTHOFINDER"]["genes_per_species_html"] = tabulate( + genes_per_species_pd, + headers=genes_per_species_pd.columns.to_list(), + tablefmt="html", + numalign="left", + showindex=False, + ) + + ## Number of species in orthogroup + num_species_orthogroup = re.findall( + r"(Number of species in orthogroup.*)", + overall_statistics, + flags=re.DOTALL, + )[0] + num_species_orthogroup_pd = pd.read_csv( + StringIO(num_species_orthogroup), sep="\t", header=0 + ) + data["ORTHOFINDER"]["num_species_orthogroup"] = num_species_orthogroup_pd.to_dict( + "records" + ) + data["ORTHOFINDER"]["num_species_orthogroup_html"] = tabulate( + num_species_orthogroup_pd, + headers=num_species_orthogroup_pd.columns.to_list(), + tablefmt="html", + numalign="left", + showindex=False, + ) + + return data diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html index c7c94f04..4da53369 100644 --- a/bin/report_modules/templates/base.html +++ b/bin/report_modules/templates/base.html @@ -79,6 +79,11 @@ {% if 'MERQURY' in all_stats_dicts %} {% endif %} + + {% if 'ORTHOFINDER' in all_stats_dicts %} + + {% endif %} + {% include 'params/params.html' %} @@ -151,6 +156,11 @@ {% if 'MERQURY' in all_stats_dicts %} {% include 'merqury/merqury.html' %} {% endif %} + + {% if 'ORTHOFINDER' in all_stats_dicts %} + {% include 'orthofinder/orthofinder.html' %} + {% endif %} + {% include 'js.html' %} diff --git a/bin/report_modules/templates/orthofinder/orthofinder.html b/bin/report_modules/templates/orthofinder/orthofinder.html new file mode 100644 index 00000000..35928c4d --- /dev/null +++ b/bin/report_modules/templates/orthofinder/orthofinder.html @@ -0,0 +1,14 @@ + diff --git a/bin/report_modules/templates/orthofinder/report_contents.html b/bin/report_modules/templates/orthofinder/report_contents.html new file mode 100644 index 00000000..8afa502a --- /dev/null +++ b/bin/report_modules/templates/orthofinder/report_contents.html @@ -0,0 +1,33 @@ +
    +
    + +
    +

    Species tree (rooted)

    +
    +
    + +
    + +
    +

    General statistics

    +
    +
    +
    {{ all_stats_dicts['ORTHOFINDER']['general_stats_html'] }}
    +
    + +
    +

    Genes per-species in orthogroup

    +
    +
    +
    {{ all_stats_dicts['ORTHOFINDER']['genes_per_species_html'] }}
    +
    + +
    +

    Number of species in orthogroup

    +
    +
    +
    {{ all_stats_dicts['ORTHOFINDER']['num_species_orthogroup_html'] }}
    +
    + +
    +
    diff --git a/conf/modules.config b/conf/modules.config index 0f05443c..090ebd3a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -388,6 +388,18 @@ process { ] } + withName: '.*:ASSEMBLYQC:GFFREAD' { + ext.args = '-y -S' + } + + withName: '.*:ASSEMBLYQC:ORTHOFINDER' { + publishDir = [ + path: { "${params.outdir}/orthofinder" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } + withName: '.*:ASSEMBLYQC:CREATEREPORT' { publishDir = [ [ diff --git a/docs/images/assemblyqc.png b/docs/images/assemblyqc.png new file mode 100644 index 0000000000000000000000000000000000000000..5701fcd97fcf6d85b4eb796310ee1c3fef65bd0d GIT binary patch literal 547780 zcmeFZWn7e7)IJR3fQUht3XCG%tspQe-4a8GG*Z$fF<68kN;lG764IcAbc=+5bR*sH z?(rOtM>ywspXdMie9+%0%zfW`?X|A8*0rvE`^d|RUBteOjfRGHQ9@i;0S)a!HX0h% z3oK0VKUx+T@6gcDX-)6llQ+93cF)S(%0}_g1AW6g23C583SxKJZ{50yhDP^VUsqQ_ 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z*7AZ+2T*qkKjY@%Y5M-%W97?7B^ZvlehI#Pide`2pEYON%goFV@dB=ujSo8(XO6_^ zuS!S<(xbJBzS@EwDhl=ZgakfVOwGCcjr=`yrCZ<2dCdPQgLPS7^CaJ)Lwq{}m-yv} z1LMkl{(SK>>SCk(tm>lbYLic&J~jUM;SIm~ap74NXk!1~2<(Cchk3+`4@E zIxZ0z854LU-(*kExL0-d31RdxH!6pO_$8}VD*I?bMr;e4Y&bQYr!$Et9q7Dw@FJcy1YKZ+$` zdGnQ3pB*566{I+_W1GJ+tm$Cl2Y2i-Y=~dG+W$WFzq|4OXl-!(UWdPTl%I=Rd%`PZ z+qRw0Rh1QVx8CUgEz#dM>VGfn|L@z^ezd`EZo7f?sa^QDV%IkKM-_Kkxj^w;!2bcs CbIHsA From fb206c4f68767c198d8b7114fbe380c915927806 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Nov 2024 13:03:00 +1300 Subject: [PATCH 49/62] Updated docs --- CHANGELOG.md | 5 +- CITATIONS.md | 46 +++++----- README.md | 53 +++++++---- docs/images/assemblyqc.png | Bin 561056 -> 581306 bytes docs/images/orthofinder.png | Bin 0 -> 107612 bytes docs/output.md | 177 ++++++++++++++++++++---------------- docs/parameters.md | 36 ++++---- docs/usage.md | 58 ++++++------ nextflow.config | 24 ++--- nextflow_schema.json | 116 +++++++++++------------ 10 files changed, 279 insertions(+), 236 deletions(-) create mode 100644 docs/images/orthofinder.png diff --git a/CHANGELOG.md b/CHANGELOG.md index fb8159fa..5203d463 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0dev - [31-Oct-2024] +## v2.2.0dev - [04-Nov-2024] ### `Added` @@ -16,6 +16,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 7. Added parameter `hic_samtools_ext_args` and set its default value to `-F 3852` [#159](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/159) 8. Added the HiC QC report to the final report so that users don't have to navigate to the results folder [#162](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/162) 9. Added the fastp log to the final report [#163](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/163) +10. Updated the tube map along with the tool list [#166](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/166) +11. Added Orthofinder [#167](https://github.com/Plant-Food-Research-Open/assemblyqc/issues/167) +12. Changed order of tool options in the `nextflow.config` file ### `Fixed` diff --git a/CITATIONS.md b/CITATIONS.md index 22ae2fb0..bf095d43 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -18,15 +18,15 @@ > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: -- SAMTOOLS, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE) +- samtools, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE) > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, -- NCBI/FCS, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt) +- NCBI FCS, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt) > Astashyn A, Tvedte ES, Sweeney D, Sapojnikov V, Bouk N, Joukov V, Mozes E, Strope PK, Sylla PM, Wagner L, Bidwell SL, Clark K, Davis EW, Smith-White B, Hlavina W, Pruitt KD, Schneider VA, Murphy TD. 2023. Rapid and sensitive detection of genome contamination at scale with FCS-GX. bioRxiv 2023.06.02.543519; doi: -- KRONA, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt) +- Krona, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt) > Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30;12:385. doi: @@ -36,7 +36,7 @@ > > Forked from: -- GFASTATS, [MIT](https://github.com/vgl-hub/gfastats/blob/main/LICENSE) +- gfastats, [MIT](https://github.com/vgl-hub/gfastats/blob/main/LICENSE) > Giulio Formenti, Linelle Abueg, Angelo Brajuka, Nadolina Brajuka, Cristóbal Gallardo-Alba, Alice Giani, Olivier Fedrigo, Erich D Jarvis, Gfastats: conversion, evaluation and manipulation of genome sequences using assembly graphs, Bioinformatics, Volume 38, Issue 17, September 2022, Pages 4214–4216, @@ -44,15 +44,15 @@ > Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. 2021. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes, Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654, -- GFFREAD, [MIT](https://github.com/gpertea/gffread/blob/master/LICENSE) +- GffRead, [MIT](https://github.com/gpertea/gffread/blob/master/LICENSE) > Pertea G, Pertea M. GFF Utilities: GffRead and GffCompare. F1000Res. 2020 Apr 28;9:ISCB Comm J-304. doi: . PMID: 32489650; PMCID: PMC7222033. -- TIDK, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE) +- tidk, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE) > -- SEQKIT, [MIT](https://github.com/shenwei356/seqkit/blob/master/LICENSE) +- SeqKit, [MIT](https://github.com/shenwei356/seqkit/blob/master/LICENSE) > Shen W, Le S, Li Y, Hu F. 2016. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation. PLoS ONE 11(10): e0163962. @@ -72,19 +72,19 @@ > Shujun O, Ning J 2018. LTR_retriever: A Highly Accurate and Sensitive Program for Identification of Long Terminal Repeat Retrotransposons, Plant Physiology, 176, 2 (2018). -- KRAKEN2, [MIT](https://github.com/DerrickWood/kraken2/blob/master/LICENSE) +- Kraken 2, [MIT](https://github.com/DerrickWood/kraken2/blob/master/LICENSE) > Wood DE, Salzberg SL, Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2. Genome Biol 20, 257 (2019). -- JUICEBOX.JS, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE) +- juicebox.js, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE) > Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. 2018. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. doi: . Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. -- FASTP, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE) +- fastp, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE) > Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor, Bioinformatics, Volume 34, Issue 17, 01 September 2018, Pages i884–i890, -- FASTQC, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt) +- FastQC, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt) > @@ -92,7 +92,7 @@ > Dudchenko O, Batra SS, Omer AD, Nyquist SK, Hoeger M, Durand NC, Shamim MS, Machol I, Lander, Aiden AP, Aiden EL 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.Science356, 92-95(2017). doi: . Available at: -- HIC_QC, [AGPL v3](https://github.com/phasegenomics/hic_qc/blob/master/LICENSE) +- hic_qc, [AGPL v3](https://github.com/phasegenomics/hic_qc/blob/master/LICENSE) > @@ -100,42 +100,46 @@ > -- BWA, [GPL v3](https://github.com/lh3/bwa/blob/master/COPYING) +- bwa-mem, [GPL v3](https://github.com/lh3/bwa/blob/master/COPYING) > Li H. 2013. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. -- MATLOCK, [AGPL v3](https://github.com/phasegenomics/matlock/blob/master/LICENSE) +- Matlock, [AGPL v3](https://github.com/phasegenomics/matlock/blob/master/LICENSE) > ; -- SAMBLASTER, [MIT](https://github.com/GregoryFaust/samblaster/blob/master/LICENSE.txt) +- samblaster, [MIT](https://github.com/GregoryFaust/samblaster/blob/master/LICENSE.txt) > Faust GG, Hall IM. 2014. SAMBLASTER: fast duplicate marking and structural variant read extraction, Bioinformatics, Volume 30, Issue 17, September 2014, Pages 2503–2505, -- CIRCOS, [GPL v3](https://www.gnu.org/licenses/gpl-3.0.txt) +- Circos, [GPL v3](https://www.gnu.org/licenses/gpl-3.0.txt) > Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R. Horsman D, ... Marra MA. 2009. Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645. -- MUMMER, [Artistic 2.0](https://github.com/mummer4/mummer/blob/master/LICENSE.md) +- MUMmer, [Artistic 2.0](https://github.com/mummer4/mummer/blob/master/LICENSE.md) > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: . PMID: 29373581; PMCID: PMC5802927. -- PLOTSR, [MIT](https://github.com/schneebergerlab/plotsr/blob/master/LICENSE) +- Plotsr, [MIT](https://github.com/schneebergerlab/plotsr/blob/master/LICENSE) > Goel M, Schneeberger K. 2022. plotsr: visualizing structural similarities and rearrangements between multiple genomes. Bioinformatics. 2022 May 13;38(10):2922-2926. doi: . PMID: 35561173; PMCID: PMC9113368. -- SYRI, [MIT](https://github.com/schneebergerlab/syri/blob/master/LICENSE) +- Syri, [MIT](https://github.com/schneebergerlab/syri/blob/master/LICENSE) > Goel M, Sun H, Jiao WB, Schneeberger K. 2019. SyRI: finding genomic rearrangements and local sequence differences from whole-genome assemblies. Genome Biol. 2019 Dec 16;20(1):277. doi: . PMID: 31842948; PMCID: PMC6913012. -- MINIMAP2, [MIT](https://github.com/lh3/minimap2/blob/master/LICENSE.txt) +- Minimap2, [MIT](https://github.com/lh3/minimap2/blob/master/LICENSE.txt) > Li H. 2021. New strategies to improve minimap2 alignment accuracy, Bioinformatics, Volume 37, Issue 23, December 2021, Pages 4572–4574, doi: -- MERQURY, [United States Government Work](https://github.com/marbl/merqury?tab=License-1-ov-file#readme) +- Merqury, [United States Government Work](https://github.com/marbl/merqury?tab=License-1-ov-file#readme) > Rhie, A., Walenz, B.P., Koren, S. et al. 2020. Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies. Genome Biol 21, 245. doi: +- OrthoFinder, [GPL v3](https://github.com/davidemms/OrthoFinder/blob/master/License.md) + + > Emms, D.M., Kelly, S. OrthoFinder: phylogenetic orthology inference for comparative genomics. Genome Biol 20, 238 (2019). doi: 10.1186/s13059-019-1832-y + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/README.md b/README.md index 2283fc0b..0b4c71e7 100644 --- a/README.md +++ b/README.md @@ -12,26 +12,43 @@ ## Introduction -**plant-food-research-open/assemblyqc** is a [Nextflow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their references are listed in [CITATIONS.md](./CITATIONS.md). +**plant-food-research-open/assemblyqc** is a [Nextflow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their references are listed in [CITATIONS.md](./CITATIONS.md). The pipeline includes skip flags to disable execution of many of it tools. ## Pipeline Flowchart

      -- [FASTA VALIDATOR](https://github.com/linsalrob/fasta_validator) + [SEQKIT RMDUP](https://github.com/shenwei356/seqkit): FASTA validation -- [GENOMETOOLS GT GFF3VALIDATOR](https://genometools.org/tools/gt_gff3validator.html): GFF3 validation -- [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl), [GFASTATS](https://github.com/vgl-hub/gfastats): Assembly statistics -- [GENOMETOOLS GT STAT](https://genometools.org/tools/gt_stat.html): Annotation statistics -- [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail -- [NCBI FCS GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail -- [BUSCO](https://gitlab.com/ezlab/busco): Gene-space completeness estimation -- [TIDK](https://github.com/tolkit/telomeric-identifier): Telomere repeat identification -- [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences -- [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification -- [HIC CONTACT MAP](https://github.com/igvteam/juicebox.js): Alignment and visualisation of HiC data -- [MUMMER](https://github.com/mummer4/mummer) → [CIRCOS](http://circos.ca/documentation/) + [DOTPLOT](https://plotly.com) & [MINIMAP2](https://github.com/lh3/minimap2) → [PLOTSR](https://github.com/schneebergerlab/plotsr): Synteny analysis -- [MERQURY](https://github.com/marbl/merqury): K-mer completeness, consensus quality and phasing assessment -- [ORTHOFINDER](https://github.com/davidemms/OrthoFinder): Phylogenetic orthology inference for comparative genomics +- `Assembly` + - [fasta_validator](https://github.com/linsalrob/fasta_validator) + [SeqKit rmdup](https://github.com/shenwei356/seqkit): FASTA validation + - [assemblathon_stats](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl), [gfastats](https://github.com/vgl-hub/gfastats): Assembly statistics + - [NCBI FCS-adaptor](https://github.com/ncbi/fcs): Adaptor contamination pass/fail + - [NCBI FCS-GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail + - [tidk](https://github.com/tolkit/telomeric-identifier): Telomere repeat identification + - [BUSCO](https://gitlab.com/ezlab/busco): Gene-space completeness estimation + - [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences + - [Kraken 2](https://github.com/DerrickWood/kraken2), [Krona](https://github.com/marbl/Krona): Taxonomy classification + - `Alignment and visualisation of HiC data` + - [sra-tools](https://github.com/ncbi/sra-tools): HiC data download from SRA or use of local FASTQ files + - [fastp](https://github.com/OpenGene/fastp), [FastQC](https://github.com/s-andrews/FastQC): QC and trimming + - [SeqKit sort](https://github.com/shenwei356/seqkit): Alphanumeric sorting of FASTA by sequence ID + - [bwa-mem](https://github.com/lh3/bwa): HiC read alignment + - [samblaster](https://github.com/GregoryFaust/samblaster): Duplicate marking + - [hic_qc](https://github.com/phasegenomics/hic_qc): HiC read and alignment statistics + - [Matlock](https://github.com/phasegenomics/matlock): BAM to juicer conversion + - [3d-dna/visualize](https://github.com/aidenlab/3d-dna/tree/master/visualize): `.hic` file creation + - [juicebox.js](https://github.com/igvteam/juicebox.js): HiC contact map visualisation + - `K-mer completeness, consensus quality and phasing assessment` + - [sra-tools](https://github.com/ncbi/sra-tools): Assembly, maternal and paternal data download from SRA or use of local FASTQ files + - [Merqury hapmers](https://github.com/marbl/merqury/blob/master/trio/hapmers.sh): Hapmer generation if parental data is available + - [Merqury](https://github.com/marbl/merqury): Completeness, consensus quality and phasing assessment + - `Synteny analysis` + - [MUMmer](https://github.com/mummer4/mummer) → [Circos](http://circos.ca/documentation/) + [dotplot](https://plotly.com): One-to-all and all-to-all synteny analysis at the contig level + - [Minimap2](https://github.com/lh3/minimap2) → [Syri](https://github.com/schneebergerlab/syri)/[Plotsr](https://github.com/schneebergerlab/plotsr): One-one to chromosome synteny analysis at the chromosome level +- `Annotation` + - [GenomeTools gt gff3validator](https://genometools.org/tools/gt_gff3validator.html) + [FASTA/GFF correspondence](subworkflows/gallvp/gff3_gt_gff3_gff3validator_stat/main.nf): GFF3 validation + - [GenomeTools gt stat](https://genometools.org/tools/gt_stat.html): Annotation statistics + - [GffRead](https://github.com/gpertea/gffread), [BUSCO](https://gitlab.com/ezlab/busco): Gene-space completeness estimation in annotation proteins + - [OrthoFinder](https://github.com/davidemms/OrthoFinder): Phylogenetic orthology inference for comparative genomics ## Usage @@ -50,9 +67,9 @@ Now, you can run the pipeline using: ```bash nextflow run plant-food-research-open/assemblyqc \ -revision \ - -profile \ - --input assemblysheet.csv \ - --outdir + -profile \ + --input assemblysheet.csv \ + --outdir ``` > [!WARNING] diff --git a/docs/images/assemblyqc.png b/docs/images/assemblyqc.png index 54ef8b9dfe39b6c8d61ad434f730f772f29066dd..3fd97dd1bca463d44c7e7f5471c9074709dfffd2 100644 GIT binary patch literal 581306 zcmeFZbzGEN_dX2cpd%q6A&np+QW7Fk4pP!cH%KF;(jYN#loAl7kr1R)xZ;bN4^_=o4LYyFG&DY6!uk9W1$u*o;A!bu zg>%qQ8v(-yO%rGu^eMHC@EvR%BRV4d;2Sq?7@`a|P12%K*=?%+UaQwV6OhHh8J=3Havix;ZKy(E`{9_`TR$c-*?R^%!sX6z%+@Zd++^aQEN9UV%Q&rXKxyy!K%~jcOPXxmC`d!J^mk0rP4C2Aqo3CAQY{{%IBkGe2=K1g5Df$JMjH#;BSt~k6lUbF`5u>czWeOcMgSP{On z!nMvsx0hCG?(sf9bN0oho@{U2nrFr%xcGh%4bh9$M&ZdOR}y!LqUZ=VK`K9xBt@x906IwNS9FjAN~~i z95z$|1`Fu_@?aFi2Q+8~zI5u{|LL#6bC>?71^@fp|C`zVwe$aDME&c}|EECy*RTHd ztKVAS-#Gd=j{buM{>6>oM$PG@{%;)p2QNDPqksMCU%&dT1^$hr)4}o|KKVC}e(Od5 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zw<~(CTeoptC$+T@h89a_GE@C$y0C16O}?e*WuJ2Yl`gtBBaXIvd81RMF5tB(u%+vq z{m;~np<8=j9OhtV=c~0Jx2^xk_=37lF+6YlmGsXHI^a(=cf;jnaclF>beo||^TGUP zkF=NmZ$FcbgfEi-UxwFj^N`xcyZl>oXlow~Em}!v`#-a=k(%f&Aww>fa-%fYM+NM{0p^usJnK4Z8@0=#;RQicaf&T+b#B419 literal 0 HcmV?d00001 diff --git a/docs/output.md b/docs/output.md index dd5dd998..68adc287 100644 --- a/docs/output.md +++ b/docs/output.md @@ -8,26 +8,27 @@ The directories listed below will be created in the results directory after the ## Pipeline overview -The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: +The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data to produce following outputs: -- [FASTA and GFF3 validation](#fasta-and-gff3-validation) +- [Format validation](#format-validation) - [Assemblathon stats](#assemblathon-stats) -- [Gfastats](#gfastats) -- [GenomeTools gt stat](#genometools-gt-stat) -- [NCBI FCS adaptor](#ncbi-fcs-adaptor) -- [NCBI FCS GX](#ncbi-fcs-gx) +- [gfastats](#gfastats) +- [NCBI FCS-adaptor](#ncbi-fcs-adaptor) +- [NCBI FCS-GX](#ncbi-fcs-gx) +- [tidk](#tidk) - [BUSCO](#busco) -- [TIDK](#tidk) - [LAI](#lai) -- [Kraken2](#kraken2) +- [Kraken 2](#kraken-2) - [HiC contact map](#hic-contact-map) -- [Synteny](#synteny) - [Merqury](#merqury) +- [Synteny](#synteny) +- [GenomeTools gt stat](#genometools-gt-stat) +- [OrthoFinder](#orthofinder) - [Pipeline information](#pipeline-information) -### FASTA and GFF3 validation +### Format validation The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fails. The error log from the validator is reported in the `report.html`. The remaining QC tools are skipped for the assembly with invalid fasta file. @@ -46,7 +47,7 @@ The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fa > [!WARNING] > Contig-related stats are based on the assumption that `assemblathon_stats_n_limit` is specified correctly. If you are not certain of the value of `assemblathon_stats_n_limit`, please ignore the contig-related stats. -### Gfastats +### gfastats

      Output files @@ -56,35 +57,21 @@ The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fa
      -Gfastats is a fast and exhaustive tool for summary statistics. +gfastats is a fast and exhaustive tool for summary statistics. -### GenomeTools gt stat - -
      -Output files - -- `genometools_gt_stat/` - - `*.gt.stat.yml`: Assembly annotation stats in yaml format. - -
      - -GenomeTools `gt stat` tool calculates a basic set of statistics about features contained in GFF3 files. - -
      AssemblyQC - GenomeTools gt stat gene length distribution
      AssemblyQC - GenomeTools gt stat gene length distribution
      - -### NCBI FCS adaptor +### NCBI FCS-adaptor
      Output files - `ncbi_fcs_adaptor/` - - `*_fcs_adaptor_report.tsv`: NCBI FCS adaptor report in CSV format. + - `*_fcs_adaptor_report.tsv`: NCBI FCS-adaptor report in CSV format.
      -[FCS-adaptor detects](https://github.com/ncbi/fcs/wiki/FCS-adaptor#rules-for-action-assignment) adaptor and vector contamination in genome sequences. +[FCS-adaptor](https://github.com/ncbi/fcs/wiki/FCS-adaptor#rules-for-action-assignment) detects adaptor and vector contamination in genome sequences. -### NCBI FCS GX +### NCBI FCS-GX
      Output files @@ -98,42 +85,47 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c
      -[FCS-GX detects](https://github.com/ncbi/fcs/wiki/FCS-GX#outputs) contamination from foreign organisms in genome sequences. +[FCS-GX](https://github.com/ncbi/fcs/wiki/FCS-GX#outputs) detects contamination from foreign organisms in genome sequences. -### BUSCO +### tidk
      Output files -- `busco/` - - `busco_figure.png`: Summary figure created from all the BUSCO summaries. - - `tag` - - `short_summary.specific.*_odb10.tag_*.txt`: BUSCO summary for the assembly represented by `tag`. +- `tidk/` + - `*.apriori.tsv`: Frequencies for successive windows in forward and reverse directions for the pre-specified telomere-repeat sequence. + - `*.apriori.svg`: Plot of `*.apriori.tsv` + - `*.tidk.explore.tsv`: List of the most frequent repeat sequences. + - `*.top.sequence.txt`: The top sequence from `*.tidk.explore.tsv`. + - `*.aposteriori.tsv`: Frequencies for successive windows in forward and reverse directions for the top sequence from `*.top.sequence.txt`. + - `*.aposteriori.svg`: Plot of `*.aposteriori.tsv`.
      -[BUSCO estimates](https://busco.ezlab.org/busco_userguide.html) the completeness and redundancy of processed genomic data based on universal single-copy orthologs. +tidk toolkit is designed to [identify and visualize](https://github.com/tolkit/telomeric-identifier) telomeric repeats for the Darwin Tree of Life genomes. -
      AssemblyQC - BUSCO summary plot
      AssemblyQC - BUSCO summary plot
      +
      AssemblyQC - tidk plot
      AssemblyQC - tidk plot
      -### TIDK +### BUSCO
      Output files -- `tidk/` - - `*.apriori.tsv`: Frequencies for successive windows in forward and reverse directions for the pre-specified telomere-repeat sequence. - - `*.apriori.svg`: Plot of `*.apriori.tsv` - - `*.tidk.explore.tsv`: List of the most frequent repeat sequences. - - `*.top.sequence.txt`: The top sequence from `*.tidk.explore.tsv`. - - `*.aposteriori.tsv`: Frequencies for successive windows in forward and reverse directions for the top sequence from `*.top.sequence.txt`. - - `*.aposteriori.svg`: Plot of `*.aposteriori.tsv`. +- `busco/` + - `fasta` + - `busco_figure.png`: Summary figure created from all the BUSCO summaries. + - `tag` + - `short_summary.specific.*_odb10.tag_*.txt`: BUSCO summary for the assembly represented by `tag`. + - `gff` + - `busco_figure.png`: Summary figure created from all the BUSCO summaries. + - `tag` + - `short_summary.specific.*_odb10.tag_*.txt`: BUSCO summary for the annotation of the assembly represented by `tag`.
      -TIDK toolkit is designed to [identify and visualize](https://github.com/tolkit/telomeric-identifier) telomeric repeats for the Darwin Tree of Life genomes. +[BUSCO](https://busco.ezlab.org/busco_userguide.html) estimates the completeness and redundancy of processed genomic data based on universal single-copy orthologs. -
      AssemblyQC - TIDK plot
      AssemblyQC - TIDK plot
      +
      AssemblyQC - BUSCO summary plot
      AssemblyQC - BUSCO summary plot
      ### LAI @@ -154,22 +146,22 @@ LTR Assembly Index (LAI) is a reference-free genome metric that [evaluates assem > [!WARNING] > Soft masked regions are unmasked when calculating LAI. However, hard masked regions are left as is. The pipeline will fail to calculate LAI if all the LTRs are already hard masked. -### Kraken2 +### Kraken 2
      Output files - `kraken2/` - - `*.kraken2.report`: [Kraken2 report](https://github.com/DerrickWood/kraken2/wiki/Manual#output-formats). - - `*.kraken2.cut`: [Kraken2 output](https://github.com/DerrickWood/kraken2/wiki/Manual#output-formats). + - `*.kraken2.report`: [Kraken 2 report](https://github.com/DerrickWood/kraken2/wiki/Manual#output-formats). + - `*.kraken2.cut`: [Kraken 2 output](https://github.com/DerrickWood/kraken2/wiki/Manual#output-formats). - `*.kraken2.krona.cut`: [Select columns](../modules/local/kraken2_krona_plot.nf) from `*.kraken2.cut` used for generation of a Krona taxonomy plot. - `*.kraken2.krona.html`: Interactive Krona taxonomy plot.
      -Kraken2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to sequencing reads for metagenomics projects. Further reading regarding performance of Kraken2: +Kraken 2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to sequencing reads for metagenomics projects. Further reading regarding performance of Kraken 2: -
      AssemblyQC - Interactive Krona plot from Kraken2 taxonomy
      AssemblyQC - Interactive Krona plot from Kraken2 taxonomy
      +
      AssemblyQC - Interactive Krona plot from Kraken 2 taxonomy
      AssemblyQC - Interactive Krona plot from Kraken 2 taxonomy
      ### HiC contact map @@ -178,17 +170,17 @@ Kraken2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to seq - `hic/` - `fastqc_raw/` - - `*_1_fastqc.html/*_2_fastqc.html`: FASTQC html report for the raw reads - - `*_1_fastqc.zip/*_2_fastqc.zip`: FASTQC stats for the raw reads + - `*_1_fastqc.html/*_2_fastqc.html`: FastQC html report for the raw reads + - `*_1_fastqc.zip/*_2_fastqc.zip`: FastQC stats for the raw reads - `fastp/` - - `*.fastp.html`: FASTP HTML report - - `*.fastp.json`: FASTP statistics in JSON format - - `*.fastp.log`: FASTP log - - `*_1.fastp.fastq.gz/*_2.fastp.fastq.gz`: Reads passed by FASTP - - `*_1.fail.fastq.gz/*_2.fail.fastq.gz`: Reads failed by FASTP + - `*.fastp.html`: fastp HTML report + - `*.fastp.json`: fastp statistics in JSON format + - `*.fastp.log`: fastp log + - `*_1.fastp.fastq.gz/*_2.fastp.fastq.gz`: Reads passed by fastp + - `*_1.fail.fastq.gz/*_2.fail.fastq.gz`: Reads failed by fastp - `fastqc_trim/` - - `*_1_fastqc.html/*_2_fastqc.html`: FASTQC html report for the reads passed by FASTP. - - `*_1_fastqc.zip/*_2_fastqc.zip`: FASTQC stats for the reads passed by FASTP. + - `*_1_fastqc.html/*_2_fastqc.html`: FastQC html report for the reads passed by FASTP. + - `*_1_fastqc.zip/*_2_fastqc.zip`: FastQC stats for the reads passed by FASTP. - `hicqc` - `*.on.*_qc_report.pdf`: HiC QC report for reads mapped to an assembly. - `assembly/` @@ -206,6 +198,28 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe AssemblyQC - HiC results +### Merqury + +
      +Output files + +- `merqury/` + - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`. + - `*.completeness.stats`: Assembly completeness statistics + - `*.qv`: Assembly consensus quality QV statistics + - `*.fl.png`: Spectra plots + - `*.hapmers.blob.png`: Hap-mer blob plot +
      + +[Merqury](https://github.com/marbl/merqury) is used for the k-mer analysis. + +
      +AssemblyQC - Spectra-cn plot +AssemblyQC - Plotsr synteny plot +
      +AssemblyQC - Merqury plots +
      + ### Synteny
      @@ -225,37 +239,42 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe - `plotsr.png`: Plotsr synteny plot
      -[Circos](https://circos.ca) and linear synteny plots are created from genome-wide alignments performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl). +[Circos](https://circos.ca) and dotplots are created from genome-wide alignments performed with [MUMmer](https://github.com/mummer4/mummer?tab=readme-ov-file). Whereas, [Plotsr](https://github.com/schneebergerlab/plotsr) plots are created from genome-wide alignments performed with [Minimap2](https://github.com/lh3/minimap2).
      AssemblyQC - Circos synteny plot AssemblyQC - Plotsr synteny plot -AssemblyQC - Dotplot synteny plot +AssemblyQC - dotplot synteny plot
      AssemblyQC - Synteny plots
      -### Merqury +### GenomeTools gt stat
      Output files -- `merqury/` - - `tag1-and-tag2`: Results folder for haplotype `tag1` and `tag2`. - - `*.completeness.stats`: Assembly completeness statistics - - `*.qv`: Assembly consensus quality QV statistics - - `*.fl.png`: Spectra plots - - `*.hapmers.blob.png`: Hap-mer blob plot -
      +- `genometools_gt_stat/` + - `*.gt.stat.yml`: Assembly annotation stats in yaml format. -[MERQURY](https://github.com/marbl/merqury) is used for the k-mer analysis. +

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interactive contact map +AssemblyQC - fastp log for HiC reads +AssemblyQC - HiC QC report +AssemblyQC - HiC interactive contact map