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I tried using fastplong to strip barcodes from long reads. I added all barcodes to a fasta file (adapter.fasta). Fastplong seems to ignore the --adapter_fasta as far as I could tell and still detects the adapter (here barcode+flanking sequences):
Command:
fastplong --in in.fastq.gz --out out.fastq.gz --adapter_fasta adapter.fasta ...
Terminal output:
Trying to detect adapter sequence at read start
Detected: AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT
Trying to detect adapter sequence at read end
Detected: AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT
...
Also I would prefer if it would be possible to specify a fasta file for the -s and -e parameters so I could define multiple sequences per position. Using only the common flanking sequence in my case remove way to many bases.
The text was updated successfully, but these errors were encountered:
Hello,
I tried using fastplong to strip barcodes from long reads. I added all barcodes to a fasta file (adapter.fasta). Fastplong seems to ignore the --adapter_fasta as far as I could tell and still detects the adapter (here barcode+flanking sequences):
Command:
fastplong --in in.fastq.gz --out out.fastq.gz --adapter_fasta adapter.fasta ...
Terminal output:
Trying to detect adapter sequence at read start
Detected: AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT
Trying to detect adapter sequence at read end
Detected: AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT
...
Adapter.fasta:
Also I would prefer if it would be possible to specify a fasta file for the -s and -e parameters so I could define multiple sequences per position. Using only the common flanking sequence in my case remove way to many bases.
The text was updated successfully, but these errors were encountered: