README.md

Comparing the Influence of Assembly Processes Governing Bacterial Community Succession Based on DNA and RNA Data

The following is the scripts related to this paper: Comparing the influence of assembly processes governing bacterial community succession based on DNA and RNA data, authored by Xiu Jia, Francisco Dini-Andreote and Joana Falcão Salles; Microorganisms 2020, 8(6), 798; https://doi.org/10.3390/microorganisms8060798

Analysis 16S rRNA sequences (DNA- and RNA-based)

Work at QIIME2/2019.10 environment, using the following script

• script_DNA_RNA_DADA2_xiu_28-11-2019.sh

Community and statistic analysis

• alpha & beta-diversity analysis:

  • alpha_diversity_dna_rna.R
  • beta_diversity_dna_rna.R

• Summarize the relative abundance of phyla:

  • taxa_summarize_dna_rna.R

• Analyze the rare biopphere:

  • Rare_biosphere_analysis_RNA_DNA.R
  • TruncateTable.R

• Community assembly analysis:

  • Raup Crick scripts updated from https://github.com/stegen/Stegen_etal_ISME_2013
  • Franction.Addembly.Processes_rna_dna.Rmd

Author

• Jia, Xiu