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Medium_2016Interlab.xml
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Medium_2016Interlab.xml
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</sbol:SequenceAnnotation>
</sbol:sequenceAnnotation>
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<sbh:ownedBy rdf:resource="https://synbiohub.org/user/jakebeal"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<obo:OBI_0001617>29928012</obo:OBI_0001617>
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<sbol:displayId>Medium_2016Interlab_SequenceConstraint1</sbol:displayId>
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<sbh:topLevel rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/jakebeal"/>
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<sbol:object rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/RBS_Component/1"/>
</sbol:SequenceConstraint>
</sbol:sequenceConstraint>
<sbol:sequenceConstraint>
<sbol:SequenceConstraint rdf:about="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Medium_2016Interlab_SequenceConstraint2/1">
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<sbol:displayId>Medium_2016Interlab_SequenceConstraint2</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/1"/>
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<sbol:subject rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/RBS_Component/1"/>
<sbol:object rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/CDS_Component/1"/>
</sbol:SequenceConstraint>
</sbol:sequenceConstraint>
<sbol:sequenceConstraint>
<sbol:SequenceConstraint rdf:about="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Medium_2016Interlab_SequenceConstraint3/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Medium_2016Interlab_SequenceConstraint3"/>
<sbol:displayId>Medium_2016Interlab_SequenceConstraint3</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/jakebeal"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<obo:OBI_0001617>29928012</obo:OBI_0001617>
<sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/>
<sbol:subject rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/CDS_Component/1"/>
<sbol:object rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Ter_Component/1"/>
</sbol:SequenceConstraint>
</sbol:sequenceConstraint>
<sbol:sequenceConstraint>
<sbol:SequenceConstraint rdf:about="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Medium_2016Interlab_SequenceConstraint/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Medium_2016Interlab_SequenceConstraint"/>
<sbol:displayId>Medium_2016Interlab_SequenceConstraint</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/jakebeal"/>
<obo:OBI_0001617>29928012</obo:OBI_0001617>
<sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/>
<sbol:subject rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Cir_Component/1"/>
<sbol:object rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016Interlab/Pro_Component/1"/>
</sbol:SequenceConstraint>
</sbol:sequenceConstraint>
<sbol:sequence rdf:resource="https://synbiohub.org/public/iGEM_2016_interlab/Medium_2016InterlabSequence/1"/>
</sbol:ComponentDefinition>
<sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_B0034/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0034"/>
<sbol:displayId>BBa_B0034</sbol:displayId>
<sbol:version>1</sbol:version>
<prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_B0034"/>
<prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/>
<dcterms:title>BBa_B0034</dcterms:title>
<dcterms:description>RBS (Elowitz 1999) -- defines RBS efficiency</dcterms:description>
<dcterms:created>2003-01-31T12:00:00Z</dcterms:created>
<dcterms:modified>2015-08-31T04:07:20Z</dcterms:modified>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0034/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/james"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<igem:partStatus>Released HQ 2013</igem:partStatus>
<sbh:mutableDescription>RBS based on Elowitz repressilator.</sbh:mutableDescription>
<igem:discontinued>false</igem:discontinued>
<igem:dominant>true</igem:dominant>
<igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/Works"/>
<igem:group_u_list>_1_</igem:group_u_list>
<igem:m_user_id>0</igem:m_user_id>
<igem:owner_id>24</igem:owner_id>
<igem:owning_group_id>7</igem:owning_group_id>
<igem:sampleStatus>In stock</igem:sampleStatus>
<igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Available"/>
<sbh:bookmark>false</sbh:bookmark>
<sbh:mutableNotes>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:([email protected])">Brian Chow</a></sbh:mutableNotes>
<sbh:star>true</sbh:star>
<dc:creator>Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.</dc:creator>
<sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/>
<sbol:role rdf:resource="http://identifiers.org/so/SO:0000139"/>
<sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/RBS"/>
<sbol:sequenceAnnotation>
<sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_B0034/annotation23325/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0034/annotation23325"/>
<sbol:displayId>annotation23325</sbol:displayId>
<sbol:version>1</sbol:version>
<dcterms:title>conserved</dcterms:title>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0034/1"/>
<sbol:location>
<sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325"/>
<sbol:displayId>range23325</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0034/1"/>
<igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/>
<sbol:start>5</sbol:start>
<sbol:end>8</sbol:end>
<sbol:orientation rdf:resource="http://sbols.org/v2#inline"/>
</sbol:Range>
</sbol:location>
<sbol:role rdf:resource="http://identifiers.org/so/SO:0000330"/>
<sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/conserved"/>
</sbol:SequenceAnnotation>
</sbol:sequenceAnnotation>
<sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_B0034_sequence/1"/>
</sbol:ComponentDefinition>
<sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_E0040/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_E0040"/>
<sbol:displayId>BBa_E0040</sbol:displayId>
<sbol:version>1</sbol:version>
<prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_E0040"/>
<prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/>
<dcterms:title>GFP</dcterms:title>
<dcterms:description>green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212</dcterms:description>
<dcterms:created>2004-09-29T11:00:00Z</dcterms:created>
<dcterms:modified>2016-01-26T02:09:38Z</dcterms:modified>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_E0040/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/james"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<igem:partStatus>Released HQ 2013</igem:partStatus>
<sbh:mutableDescription>GFP (mut3b) [note that this part does not have a barcode]</sbh:mutableDescription>
<igem:discontinued>false</igem:discontinued>
<igem:dominant>true</igem:dominant>
<igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/Works"/>
<igem:group_u_list>_11_1_</igem:group_u_list>
<igem:m_user_id>4206</igem:m_user_id>
<igem:owner_id>61</igem:owner_id>
<igem:owning_group_id>7</igem:owning_group_id>
<igem:sampleStatus>In stock</igem:sampleStatus>
<igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Available"/>
<sbh:bookmark>false</sbh:bookmark>
<sbh:star>true</sbh:star>
<dc:creator>jcbraff</dc:creator>
<sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/>
<sbol:role rdf:resource="http://identifiers.org/so/SO:0000316"/>
<sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/Coding"/>
<sbol:sequenceAnnotation>
<sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_E0040/annotation1934520"/>
<sbol:displayId>annotation1934520</sbol:displayId>
<sbol:version>1</sbol:version>
<dcterms:title>GFP protein</dcterms:title>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_E0040/1"/>
<sbol:location>
<sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520"/>
<sbol:displayId>range1934520</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_E0040/1"/>
<igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/>
<sbol:start>1</sbol:start>
<sbol:end>720</sbol:end>
<sbol:orientation rdf:resource="http://sbols.org/v2#inline"/>
</sbol:Range>
</sbol:location>
<sbol:role rdf:resource="http://identifiers.org/so/SO:0000316"/>
<sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/protein"/>
</sbol:SequenceAnnotation>
</sbol:sequenceAnnotation>
<sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_E0040_sequence/1"/>
</sbol:ComponentDefinition>
<sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_B0015/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0015"/>
<sbol:displayId>BBa_B0015</sbol:displayId>
<sbol:version>1</sbol:version>
<prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_B0015"/>
<prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/>
<dcterms:title>BBa_B0015</dcterms:title>
<dcterms:description>double terminator (B0010-B0012)</dcterms:description>
<dcterms:created>2003-07-16T11:00:00Z</dcterms:created>
<dcterms:modified>2015-08-31T04:07:20Z</dcterms:modified>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/james"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<igem:partStatus>Released HQ 2013</igem:partStatus>
<sbh:mutableDescription>Double terminator consisting of BBa_B0010 and BBa_B0012</sbh:mutableDescription>
<igem:discontinued>false</igem:discontinued>
<igem:dominant>true</igem:dominant>
<igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/Works"/>
<igem:group_u_list>_1_</igem:group_u_list>
<igem:m_user_id>0</igem:m_user_id>
<igem:owner_id>24</igem:owner_id>
<igem:owning_group_id>7</igem:owning_group_id>
<igem:sampleStatus>In stock</igem:sampleStatus>
<igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Available"/>
<sbh:bookmark>false</sbh:bookmark>
<sbh:star>true</sbh:star>
<dc:creator>Reshma Shetty</dc:creator>
<sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/>
<sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/Terminator"/>
<sbol:role rdf:resource="http://identifiers.org/so/SO:0000141"/>
<sbol:component>
<sbol:Component rdf:about="https://synbiohub.org/public/igem/BBa_B0015/component1916612/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/component1916612"/>
<sbol:displayId>component1916612</sbol:displayId>
<sbol:version>1</sbol:version>
<dcterms:title>BBa_B0012</dcterms:title>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B0012/1"/>
<sbol:access rdf:resource="http://sbols.org/v2#public"/>
</sbol:Component>
</sbol:component>
<sbol:component>
<sbol:Component rdf:about="https://synbiohub.org/public/igem/BBa_B0015/component1916610/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/component1916610"/>
<sbol:displayId>component1916610</sbol:displayId>
<sbol:version>1</sbol:version>
<dcterms:title>BBa_B0010</dcterms:title>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B0010/1"/>
<sbol:access rdf:resource="http://sbols.org/v2#public"/>
</sbol:Component>
</sbol:component>
<sbol:sequenceAnnotation>
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<sbol:displayId>annotation1916610</sbol:displayId>
<sbol:version>1</sbol:version>
<dcterms:title>BBa_B0010</dcterms:title>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<sbol:location>
<sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610"/>
<sbol:displayId>range1916610</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/>
<sbol:start>1</sbol:start>
<sbol:end>80</sbol:end>
<sbol:orientation rdf:resource="http://sbols.org/v2#inline"/>
</sbol:Range>
</sbol:location>
<sbol:component rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/component1916610/1"/>
</sbol:SequenceAnnotation>
</sbol:sequenceAnnotation>
<sbol:sequenceAnnotation>
<sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/annotation1916612"/>
<sbol:displayId>annotation1916612</sbol:displayId>
<sbol:version>1</sbol:version>
<dcterms:title>BBa_B0012</dcterms:title>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<sbol:location>
<sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612"/>
<sbol:displayId>range1916612</sbol:displayId>
<sbol:version>1</sbol:version>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/1"/>
<igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/>
<sbol:start>89</sbol:start>
<sbol:end>129</sbol:end>
<sbol:orientation rdf:resource="http://sbols.org/v2#inline"/>
</sbol:Range>
</sbol:location>
<sbol:component rdf:resource="https://synbiohub.org/public/igem/BBa_B0015/component1916612/1"/>
</sbol:SequenceAnnotation>
</sbol:sequenceAnnotation>
<sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_B0015_sequence/1"/>
</sbol:ComponentDefinition>
<sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_J23106/1">
<sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J23106"/>
<sbol:displayId>BBa_J23106</sbol:displayId>
<sbol:version>1</sbol:version>
<prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_J23106"/>
<prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/>
<dcterms:title>BBa_J23106</dcterms:title>
<dcterms:description>constitutive promoter family member</dcterms:description>
<dcterms:created>2006-08-13T11:00:00Z</dcterms:created>
<dcterms:modified>2015-08-31T04:08:40Z</dcterms:modified>
<sbh:mutableProvenance>Isolated from library of promoters</sbh:mutableProvenance>
<sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_J23106/1"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/james"/>
<sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
<igem:partStatus>Released HQ 2013</igem:partStatus>
<sbh:mutableDescription>Later</sbh:mutableDescription>
<igem:discontinued>false</igem:discontinued>
<igem:dominant>true</igem:dominant>
<igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/Works"/>
<igem:group_u_list>_52_</igem:group_u_list>
<igem:m_user_id>0</igem:m_user_id>
<igem:owner_id>483</igem:owner_id>
<igem:owning_group_id>95</igem:owning_group_id>
<igem:sampleStatus>In stock</igem:sampleStatus>
<igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Available"/>
<sbh:bookmark>true</sbh:bookmark>
<sbh:mutableNotes>N/A</sbh:mutableNotes>
<sbh:star>true</sbh:star>
<dc:creator>John Anderson</dc:creator>
<sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/>
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<dcterms:title>pSB1C3</dcterms:title>
<dcterms:description>High copy BioBrick assembly plasmid</dcterms:description>
<dcterms:created>2008-09-07T11:00:00Z</dcterms:created>
<dcterms:modified>2015-05-08T01:14:55Z</dcterms:modified>
<sbh:mutableProvenance>This part was derived from pSB1AC3-P1010.</sbh:mutableProvenance>
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<sbh:mutableDescription>This is a high copy plasmid for use in assembly of BioBrick&reg; standard biological parts.</sbh:mutableDescription>
<igem:discontinued>false</igem:discontinued>
<igem:dominant>false</igem:dominant>
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<igem:sampleStatus>It's complicated</igem:sampleStatus>
<igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Planning"/>
<sbh:bookmark>false</sbh:bookmark>
<sbh:mutableNotes>Eliminated ampicillin resistance.</sbh:mutableNotes>
<sbh:star>true</sbh:star>
<dc:creator>Austin Che</dc:creator>
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<dcterms:title>BBa_B0012</dcterms:title>
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<sbh:mutableNotes><P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.</sbh:mutableNotes>
<sbh:star>false</sbh:star>
<dc:creator>Reshma Shetty</dc:creator>
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<dcterms:title>BBa_B0010</dcterms:title>
<dcterms:description>T1 from E. coli rrnB</dcterms:description>
<dcterms:created>2003-11-19T12:00:00Z</dcterms:created>
<dcterms:modified>2015-08-31T04:07:20Z</dcterms:modified>
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<dc:creator>Randy Rettberg</dc:creator>
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