diff --git a/tools/xls2tsv/.shed.yml b/tools/xls2tsv/.shed.yml
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+categories:
+  - Text Manipulation
+description: Convert an xlsx file to a tabular
+long_description: Extract one sheet from an xlsx/xls file and convert it to a tabular format
+name: xlsx2tsv
+owner: ufz
+homepage_url: https://github.com/Helmholtz-UFZ/galaxy-tools
+remote_repository_url: https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/xls2tsv
diff --git a/tools/xls2tsv/test-data/excel_test.xlsx b/tools/xls2tsv/test-data/excel_test.xlsx
new file mode 100644
index 00000000..b9428aa9
Binary files /dev/null and b/tools/xls2tsv/test-data/excel_test.xlsx differ
diff --git a/tools/xls2tsv/test-data/output_sheet_1.tsv b/tools/xls2tsv/test-data/output_sheet_1.tsv
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index 00000000..7c040524
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+++ b/tools/xls2tsv/test-data/output_sheet_1.tsv
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+column0	column1
+test1	value1
+test2	value2
+test3	value3
diff --git a/tools/xls2tsv/test-data/output_sheet_2.tsv b/tools/xls2tsv/test-data/output_sheet_2.tsv
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index 00000000..b4ddb905
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+++ b/tools/xls2tsv/test-data/output_sheet_2.tsv
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+column2	column3
+test4	value4
+test5	value5
+test6	value6
diff --git a/tools/xls2tsv/xlsx2tsv.py b/tools/xls2tsv/xlsx2tsv.py
new file mode 100644
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+++ b/tools/xls2tsv/xlsx2tsv.py
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+import argparse
+
+import pandas as pd
+
+
+def convert_xlsx_to_tsv(input_file, sheet_name, output):
+    try:
+        # Read the specified sheet and convert them to tsv
+        df = pd.read_excel(input_file, sheet_name=sheet_name)
+        df.to_csv(output, sep='\t', index=False)
+        print(f"Extracted sheet '{sheet_name}' from {input_file}")
+
+    except Exception as e:
+        print(f"Failed to convert sheet '{sheet_name}' from {input_file}: {e}")
+
+
+def main():
+    parser = argparse.ArgumentParser(description="Convert specific sheets from a single .xlsx file to .tsv format in the same directory.")
+    parser.add_argument("--input-file", type=str, required=True, help="Path to the input .xlsx file.")
+    parser.add_argument("--sheet-names", type=str, required=True, help="Comma-separated list of sheet names to convert.")
+    parser.add_argument("--output", type=str, default="extracted_sheet.tsv", required=False, help="Suffix for the tsv file")
+    args = parser.parse_args()
+
+    # Convert sheet names from str to list
+    sheet_names = args.sheet_names
+
+    # Call the conversion function with the provided arguments
+    convert_xlsx_to_tsv(args.input_file, sheet_names, args.output)
+
+
+if __name__ == "__main__":
+    main()
diff --git a/tools/xls2tsv/xlsx2tsv.xml b/tools/xls2tsv/xlsx2tsv.xml
new file mode 100644
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+++ b/tools/xls2tsv/xlsx2tsv.xml
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+<tool id="xlsx2tsv" name="Excel to Tabular" version="0.1.0+galaxy0" license="MIT" profile = "23.0">
+    <description>with pandas</description>
+    <requirements>
+        <requirement type="package" version="2.2.1">pandas</requirement>
+        <requirement type="package" version="3.1.5">openpyxl</requirement>
+    </requirements>
+    <command detect_errors="aggressive"><![CDATA[
+    python '$__tool_directory__/xlsx2tsv.py'
+    --input-file '$input_file'
+    --sheet-names '$sheet_names'
+    --output '$output'
+    ]]></command>
+    <inputs>
+        <param name="input_file" type="data" format="excel.xls,xlsx" optional="false" label="Input excel file" help="Input XLS/XLSX file"/>
+        <param name="sheet_names" type="text" optional="false" label="Name of the excel sheet" help="Excel sheet to convert to tsv"/>
+    </inputs>
+    <outputs>
+        <data name="output" format="tabular"/>
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_file" value="excel_test.xlsx"/>
+            <param name="sheet_names" value="Sheet1"/>
+            <output name="output" value="output_sheet_1.tsv" ftype="tabular">
+                <assert_contents>
+                        <has_text text="column0"/>
+                        <has_n_columns n="2"/>
+                    </assert_contents>
+                </output>
+        </test>
+        <test>
+            <param name="input_file" value="excel_test.xlsx"/>
+            <param name="sheet_names" value="Sheet2"/>
+            <output name="output" value="output_sheet_2.tsv" ftype="tabular">
+                <assert_contents>
+                    <has_text text="column2"/>
+                    <has_n_columns n="2"/>
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    <help>
+Description
+-----------
+Extract a sheet from XLS/XLSX file to a tabular file
+    </help>
+    <citations>
+        <citation type="doi">10.5281/zenodo.13819579</citation>
+    </citations>
+</tool>
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