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I wasn't unable to run SEACR (top 0.01 run just fine) when I use control bedgraphs with the following command: bash SEACR_1.3.sh $sample_bed $control_bed non stringent ${sample}_seacr_ctrl.peak
Using stringent threshold
Creating experimental AUC file: Fri Jun 3 03:17:29 PDT 2022
Creating control AUC file: Fri Jun 3 03:18:20 PDT 2022
Calculating optimal AUC threshold: Fri Jun 3 03:18:30 PDT 2022
Calculating threshold using non-normalized control: Fri Jun 3 03:18:30 PDT 2022
Creating thresholded feature file: Fri Jun 3 03:19:41 PDT 2022
Empirical false discovery rate = 7.62799780318346e-06
Merging nearby features and eliminating control-enriched features: Fri Jun 3 03:19:42 PDT 2022
Error: Unable to determine type for file oS9kQIRuTq0Ya.auc.threshold.bed
The bed file in question either seems to be either corrupted or something in binary.
The text was updated successfully, but these errors were encountered:
I'm guessing this is an input issue because there are no compression steps in the SEACR code--is it possible you're inputting a control bigwig instead of a bedgraph? It's possible that wouldn't be caught.
I wasn't unable to run SEACR (top 0.01 run just fine) when I use control bedgraphs with the following command:
bash SEACR_1.3.sh $sample_bed $control_bed non stringent ${sample}_seacr_ctrl.peak
The bed file in question either seems to be either corrupted or something in binary.
The text was updated successfully, but these errors were encountered: