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Flow chart.xml
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Flow chart.xml
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<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<?mso-application progid="Word.Document"?>
<pkg:package xmlns:pkg="http://schemas.microsoft.com/office/2006/xmlPackage"><pkg:part pkg:name="/_rels/.rels" pkg:contentType="application/vnd.openxmlformats-package.relationships+xml" pkg:padding="512"><pkg:xmlData><Relationships xmlns="http://schemas.openxmlformats.org/package/2006/relationships"><Relationship Id="rId3" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/extended-properties" Target="docProps/app.xml"/><Relationship Id="rId2" Type="http://schemas.openxmlformats.org/package/2006/relationships/metadata/core-properties" Target="docProps/core.xml"/><Relationship Id="rId1" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/officeDocument" Target="word/document.xml"/></Relationships></pkg:xmlData></pkg:part><pkg:part pkg:name="/word/_rels/document.xml.rels" pkg:contentType="application/vnd.openxmlformats-package.relationships+xml" pkg:padding="256"><pkg:xmlData><Relationships xmlns="http://schemas.openxmlformats.org/package/2006/relationships"><Relationship Id="rId8" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/theme" Target="theme/theme1.xml"/><Relationship Id="rId3" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/styles" Target="styles.xml"/><Relationship Id="rId7" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/fontTable" Target="fontTable.xml"/><Relationship Id="rId2" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/numbering" Target="numbering.xml"/><Relationship Id="rId1" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/customXml" Target="../customXml/item1.xml"/><Relationship Id="rId6" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/webSettings" Target="webSettings.xml"/><Relationship Id="rId5" Type="http://schemas.openxmlformats.org/officeDocument/2006/relationships/settings" Target="settings.xml"/><Relationship Id="rId4" Type="http://schemas.microsoft.com/office/2007/relationships/stylesWithEffects" Target="stylesWithEffects.xml"/></Relationships></pkg:xmlData></pkg:part><pkg:part pkg:name="/word/document.xml" pkg:contentType="application/vnd.openxmlformats-officedocument.wordprocessingml.document.main+xml"><pkg:xmlData><w:document mc:Ignorable="w14 wp14" xmlns:wpc="http://schemas.microsoft.com/office/word/2010/wordprocessingCanvas" xmlns:mc="http://schemas.openxmlformats.org/markup-compatibility/2006" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:r="http://schemas.openxmlformats.org/officeDocument/2006/relationships" xmlns:m="http://schemas.openxmlformats.org/officeDocument/2006/math" xmlns:v="urn:schemas-microsoft-com:vml" xmlns:wp14="http://schemas.microsoft.com/office/word/2010/wordprocessingDrawing" xmlns:wp="http://schemas.openxmlformats.org/drawingml/2006/wordprocessingDrawing" xmlns:w10="urn:schemas-microsoft-com:office:word" xmlns:w="http://schemas.openxmlformats.org/wordprocessingml/2006/main" xmlns:w14="http://schemas.microsoft.com/office/word/2010/wordml" xmlns:wpg="http://schemas.microsoft.com/office/word/2010/wordprocessingGroup" xmlns:wpi="http://schemas.microsoft.com/office/word/2010/wordprocessingInk" xmlns:wne="http://schemas.microsoft.com/office/word/2006/wordml" xmlns:wps="http://schemas.microsoft.com/office/word/2010/wordprocessingShape"><w:body><w:bookmarkStart w:id="0" w:name="_GoBack"/><w:bookmarkEnd w:id="0"/><w:p w:rsidR="00C23A02" w:rsidRDefault="006400EA" w:rsidP="00903B82"><w:pPr><w:pStyle w:val="a3"/><w:ind w:left="360"/><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:r><w:rPr><w:noProof/><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><mc:AlternateContent><mc:Choice Requires="wpg"><w:drawing><wp:anchor distT="0" distB="0" distL="114300" distR="114300" simplePos="0" relativeHeight="251693056" behindDoc="0" locked="0" layoutInCell="1" allowOverlap="1"><wp:simplePos x="0" y="0"/><wp:positionH relativeFrom="column"><wp:posOffset>-477276</wp:posOffset></wp:positionH><wp:positionV relativeFrom="paragraph"><wp:posOffset>-430383</wp:posOffset></wp:positionV><wp:extent cx="7525874" cy="10257692"/><wp:effectExtent l="0" t="0" r="18415" b="29845"/><wp:wrapNone/><wp:docPr id="34" name="Группа 34"/><wp:cNvGraphicFramePr/><a:graphic xmlns:a="http://schemas.openxmlformats.org/drawingml/2006/main"><a:graphicData uri="http://schemas.microsoft.com/office/word/2010/wordprocessingGroup"><wpg:wgp><wpg:cNvGrpSpPr/><wpg:grpSpPr><a:xfrm><a:off x="0" y="0"/><a:ext cx="7525874" cy="10257692"/><a:chOff x="0" y="0"/><a:chExt cx="7525874" cy="10257692"/></a:xfrm></wpg:grpSpPr><wps:wsp><wps:cNvPr id="1" name="Скругленный прямоугольник 1"/><wps:cNvSpPr/><wps:spPr><a:xfrm><a:off x="656493" y="914400"/><a:ext cx="2543810" cy="808355"/></a:xfrm><a:prstGeom prst="roundRect"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="2"><a:schemeClr val="accent1"><a:shade val="50000"/></a:schemeClr></a:lnRef><a:fillRef idx="1"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="lt1"/></a:fontRef></wps:style><wps:txbx><w:txbxContent><w:p w:rsidR="00BC6FDB" w:rsidRDefault="00BC6FDB" w:rsidP="00CE2A6C"><w:pPr><w:pStyle w:val="a3"/><w:ind w:left="360"/><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:proofErr w:type="gramStart"/><w:r w:rsidRPr="00265DB8"><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>Bacterial culture (suspension)</w:t></w:r><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>.</w:t></w:r><w:proofErr w:type="gramEnd"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve"> R</w:t></w:r><w:r w:rsidRPr="00265DB8"><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve">emove a </w:t></w:r><w:proofErr w:type="spellStart"/><w:r w:rsidRPr="00265DB8"><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>loopful</w:t></w:r><w:proofErr w:type="spellEnd"/><w:r w:rsidRPr="00265DB8"><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve"> of bacteria from tube</w:t></w:r></w:p><w:p w:rsidR="00BC6FDB" w:rsidRDefault="00BC6FDB" w:rsidP="00BC6FDB"><w:pPr><w:jc w:val="center"/></w:pPr></w:p></w:txbxContent></wps:txbx><wps:bodyPr rot="0" spcFirstLastPara="0" vertOverflow="overflow" horzOverflow="overflow" vert="horz" wrap="square" lIns="91440" tIns="45720" rIns="91440" bIns="45720" numCol="1" spcCol="0" rtlCol="0" fromWordArt="0" anchor="ctr" anchorCtr="0" forceAA="0" compatLnSpc="1"><a:prstTxWarp prst="textNoShape"><a:avLst/></a:prstTxWarp><a:noAutofit/></wps:bodyPr></wps:wsp><wps:wsp><wps:cNvPr id="11" name="Овал 11"/><wps:cNvSpPr/><wps:spPr><a:xfrm><a:off x="1148862" y="0"/><a:ext cx="1898650" cy="655955"/></a:xfrm><a:prstGeom prst="ellipse"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="2"><a:schemeClr val="accent1"><a:shade val="50000"/></a:schemeClr></a:lnRef><a:fillRef idx="1"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="lt1"/></a:fontRef></wps:style><wps:txbx><w:txbxContent><w:p w:rsidR="00903B82" w:rsidRDefault="00903B82" w:rsidP="00903B82"><w:pPr><w:jc w:val="center"/></w:pPr><w:r><w:t>Begin</w:t></w:r></w:p></w:txbxContent></wps:txbx><wps:bodyPr rot="0" spcFirstLastPara="0" vertOverflow="overflow" horzOverflow="overflow" vert="horz" wrap="square" lIns="91440" tIns="45720" rIns="91440" bIns="45720" numCol="1" spcCol="0" rtlCol="0" fromWordArt="0" anchor="ctr" anchorCtr="0" forceAA="0" compatLnSpc="1"><a:prstTxWarp prst="textNoShape"><a:avLst/></a:prstTxWarp><a:noAutofit/></wps:bodyPr></wps:wsp><wps:wsp><wps:cNvPr id="13" name="Прямая со стрелкой 13"/><wps:cNvCnPr/><wps:spPr><a:xfrm><a:off x="2004646" y="562707"/><a:ext cx="0" cy="375285"/></a:xfrm><a:prstGeom prst="straightConnector1"><a:avLst/></a:prstGeom><a:ln><a:tailEnd type="arrow"/></a:ln></wps:spPr><wps:style><a:lnRef idx="1"><a:schemeClr val="accent1"/></a:lnRef><a:fillRef idx="0"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="tx1"/></a:fontRef></wps:style><wps:bodyPr/></wps:wsp><wps:wsp><wps:cNvPr id="14" name="Прямая со стрелкой 14"/><wps:cNvCnPr/><wps:spPr><a:xfrm><a:off x="2028093" y="1512276"/><a:ext cx="0" cy="468630"/></a:xfrm><a:prstGeom prst="straightConnector1"><a:avLst/></a:prstGeom><a:ln><a:tailEnd type="arrow"/></a:ln></wps:spPr><wps:style><a:lnRef idx="1"><a:schemeClr val="accent1"/></a:lnRef><a:fillRef idx="0"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="tx1"/></a:fontRef></wps:style><wps:bodyPr/></wps:wsp><wps:wsp><wps:cNvPr id="17" name="Прямая со стрелкой 17"/><wps:cNvCnPr/><wps:spPr><a:xfrm flipV="1"><a:off x="3774831" y="6951784"/><a:ext cx="691515" cy="0"/></a:xfrm><a:prstGeom prst="straightConnector1"><a:avLst/></a:prstGeom><a:ln><a:tailEnd type="arrow"/></a:ln></wps:spPr><wps:style><a:lnRef idx="1"><a:schemeClr val="accent1"/></a:lnRef><a:fillRef idx="0"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="tx1"/></a:fontRef></wps:style><wps:bodyPr/></wps:wsp><wps:wsp><wps:cNvPr id="4" name="Ромб 4"/><wps:cNvSpPr/><wps:spPr><a:xfrm><a:off x="4829908" y="2708030"/><a:ext cx="2215515" cy="1547446"/></a:xfrm><a:prstGeom prst="diamond"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="2"><a:schemeClr val="accent6"><a:shade val="50000"/></a:schemeClr></a:lnRef><a:fillRef idx="1"><a:schemeClr val="accent6"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent6"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="lt1"/></a:fontRef></wps:style><wps:txbx><w:txbxContent><w:p w:rsidR="00121A12" w:rsidRPr="00121A12" w:rsidRDefault="00121A12" w:rsidP="00121A12"><w:pPr><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:r w:rsidRPr="00121A12"><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>Dry the smear over the fire.</w:t></w:r></w:p><w:p w:rsidR="00121A12" w:rsidRDefault="00121A12" w:rsidP="00121A12"><w:pPr><w:jc w:val="center"/></w:pPr></w:p></w:txbxContent></wps:txbx><wps:bodyPr rot="0" spcFirstLastPara="0" vertOverflow="overflow" horzOverflow="overflow" vert="horz" wrap="square" lIns="91440" tIns="45720" rIns="91440" bIns="45720" numCol="1" spcCol="0" rtlCol="0" fromWordArt="0" anchor="ctr" anchorCtr="0" forceAA="0" compatLnSpc="1"><a:prstTxWarp prst="textNoShape"><a:avLst/></a:prstTxWarp><a:noAutofit/></wps:bodyPr></wps:wsp><wps:wsp><wps:cNvPr id="5" name="Ромб 5"/><wps:cNvSpPr/><wps:spPr><a:xfrm><a:off x="4489939" y="6072553"/><a:ext cx="3035935" cy="1887220"/></a:xfrm><a:prstGeom prst="diamond"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="2"><a:schemeClr val="accent6"><a:shade val="50000"/></a:schemeClr></a:lnRef><a:fillRef idx="1"><a:schemeClr val="accent6"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent6"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="lt1"/></a:fontRef></wps:style><wps:txbx><w:txbxContent><w:p w:rsidR="00121A12" w:rsidRPr="00121A12" w:rsidRDefault="00121A12" w:rsidP="00121A12"><w:pPr><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:r w:rsidRPr="00121A12"><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve"> Cover the smear with crystal violet solution without drying smear.</w:t></w:r></w:p><w:p w:rsidR="00121A12" w:rsidRDefault="00121A12" w:rsidP="00121A12"><w:pPr><w:pStyle w:val="a3"/><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:proofErr w:type="spellStart"/><w:proofErr w:type="gramStart"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>ith</w:t></w:r><w:proofErr w:type="spellEnd"/><w:proofErr w:type="gramEnd"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve"> crystal violet solution without drying smear.</w:t></w:r></w:p><w:p w:rsidR="00121A12" w:rsidRDefault="00121A12" w:rsidP="00121A12"><w:pPr><w:jc w:val="center"/></w:pPr></w:p></w:txbxContent></wps:txbx><wps:bodyPr rot="0" spcFirstLastPara="0" vertOverflow="overflow" horzOverflow="overflow" vert="horz" wrap="square" lIns="91440" tIns="45720" rIns="91440" bIns="45720" numCol="1" spcCol="0" rtlCol="0" fromWordArt="0" anchor="ctr" anchorCtr="0" forceAA="0" compatLnSpc="1"><a:prstTxWarp prst="textNoShape"><a:avLst/></a:prstTxWarp><a:noAutofit/></wps:bodyPr></wps:wsp><wps:wsp><wps:cNvPr id="23" name="Ромб 23"/><wps:cNvSpPr/><wps:spPr><a:xfrm><a:off x="0" y="1969476"/><a:ext cx="4044315" cy="2930770"/></a:xfrm><a:prstGeom prst="diamond"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="2"><a:schemeClr val="accent1"><a:shade val="50000"/></a:schemeClr></a:lnRef><a:fillRef idx="1"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="lt1"/></a:fontRef></wps:style><wps:txbx><w:txbxContent><w:p w:rsidR="006E63D1" w:rsidRDefault="006E63D1" w:rsidP="006E63D1"><w:pPr><w:pStyle w:val="a3"/><w:ind w:left="0"/><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:proofErr w:type="gramStart"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>Glass slide.</w:t></w:r><w:proofErr w:type="gramEnd"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve"> Place the </w:t></w:r><w:proofErr w:type="spellStart"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>loopful</w:t></w:r><w:proofErr w:type="spellEnd"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t xml:space="preserve"> of bacteria in the center of the target circle on the slide. Spread the suspension of bacteria over the slide center. </w:t></w:r><w:proofErr w:type="gramStart"/><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>Dry smear in air.</w:t></w:r><w:proofErr w:type="gramEnd"/></w:p><w:p w:rsidR="006E63D1" w:rsidRDefault="006E63D1" w:rsidP="006E63D1"><w:pPr><w:jc w:val="center"/></w:pPr></w:p></w:txbxContent></wps:txbx><wps:bodyPr rot="0" spcFirstLastPara="0" vertOverflow="overflow" horzOverflow="overflow" vert="horz" wrap="square" lIns="91440" tIns="45720" rIns="91440" bIns="45720" numCol="1" spcCol="0" rtlCol="0" fromWordArt="0" anchor="ctr" anchorCtr="0" forceAA="0" compatLnSpc="1"><a:prstTxWarp prst="textNoShape"><a:avLst/></a:prstTxWarp><a:noAutofit/></wps:bodyPr></wps:wsp><wps:wsp><wps:cNvPr id="24" name="Прямая со стрелкой 24"/><wps:cNvCnPr/><wps:spPr><a:xfrm><a:off x="3774831" y="3423138"/><a:ext cx="1054735" cy="34925"/></a:xfrm><a:prstGeom prst="straightConnector1"><a:avLst/></a:prstGeom><a:ln><a:tailEnd type="arrow"/></a:ln></wps:spPr><wps:style><a:lnRef idx="1"><a:schemeClr val="accent1"/></a:lnRef><a:fillRef idx="0"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="tx1"/></a:fontRef></wps:style><wps:bodyPr/></wps:wsp><wps:wsp><wps:cNvPr id="25" name="Ромб 25"/><wps:cNvSpPr/><wps:spPr><a:xfrm><a:off x="222739" y="5310553"/><a:ext cx="3691890" cy="3340735"/></a:xfrm><a:prstGeom prst="diamond"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="2"><a:schemeClr val="accent1"><a:shade val="50000"/></a:schemeClr></a:lnRef><a:fillRef idx="1"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="lt1"/></a:fontRef></wps:style><wps:txbx><w:txbxContent><w:p w:rsidR="006E63D1" w:rsidRDefault="006E63D1" w:rsidP="006E63D1"><w:pPr><w:pStyle w:val="a3"/><w:ind w:left="360"/><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr></w:pPr><w:r><w:rPr><w:sz w:val="24"/><w:szCs w:val="24"/></w:rPr><w:t>Cover the smear with crystal violet solution and let stand for 30-60 seconds. Briefly wash of stain with distilled water and dry the slide.</w:t></w:r></w:p><w:p w:rsidR="006E63D1" w:rsidRDefault="006E63D1" w:rsidP="006E63D1"><w:pPr><w:jc w:val="center"/></w:pPr></w:p></w:txbxContent></wps:txbx><wps:bodyPr rot="0" spcFirstLastPara="0" vertOverflow="overflow" horzOverflow="overflow" vert="horz" wrap="square" lIns="91440" tIns="45720" rIns="91440" bIns="45720" numCol="1" spcCol="0" rtlCol="0" fromWordArt="0" anchor="ctr" anchorCtr="0" forceAA="0" compatLnSpc="1"><a:prstTxWarp prst="textNoShape"><a:avLst/></a:prstTxWarp><a:noAutofit/></wps:bodyPr></wps:wsp><wps:wsp><wps:cNvPr id="29" name="Прямая соединительная линия 29"/><wps:cNvCnPr/><wps:spPr><a:xfrm><a:off x="2074985" y="8639907"/><a:ext cx="35364" cy="1617785"/></a:xfrm><a:prstGeom prst="line"><a:avLst/></a:prstGeom></wps:spPr><wps:style><a:lnRef idx="1"><a:schemeClr val="accent1"/></a:lnRef><a:fillRef idx="0"><a:schemeClr val="accent1"/></a:fillRef><a:effectRef idx="0"><a:schemeClr val="accent1"/></a:effectRef><a:fontRef idx="minor"><a:schemeClr val="tx1"/></a:fontRef></wps:style><wps:bodyPr/></wps:wsp></wpg:wgp></a:graphicData></a:graphic></wp:anchor></w:drawing></mc:Choice><mc:Fallback><w:pict><v:group id="Группа 34" o:spid="_x0000_s1026" style="position:absolute;left:0;text-align:left;margin-left:-37.6pt;margin-top:-33.9pt;width:592.6pt;height:807.7pt;z-index:251693056" coordsize="75258,102576" o:gfxdata="UEsDBBQABgAIAAAAIQC2gziS/gAAAOEBAAATAAAAW0NvbnRlbnRfVHlwZXNdLnhtbJSRQU7DMBBF
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