From b78f2d99539182362598d073867608cbce1e1206 Mon Sep 17 00:00:00 2001 From: Sophie THEIS Date: Wed, 27 Dec 2023 23:46:49 +0000 Subject: [PATCH] Solving mesh collision (#263) * Fix for Issues #261 - cell division failed at the border * Fix Mesh creation Surface mesh create wasn't create all faces. Now it is fixed. Also : -add two methods to Mesh to list faces and vertices. -generalised coordinates -add `write_polygon_mesh` to export mesh into .ply to visualised with blinder * Add 2D collision solver Add class to solve collision in 2D or in 3D. 3D collision solver need to be fixed * Update README.md Add `How to cite` section Add `Geometry` section Add `Publications` section Update bibliography link * Generalised wich vertex penetrate face * Attempt to set out particular cases * Detect and fix self-crossing face Unable to make this algo works... https://europepmc.org/article/PMC/3660981 Use ordered vertices and their angle position. -> uncrossed face : angles are monotonically increasing -> crossed face : angles are not monotonically increasing * Use `.apply()` Need to recalculate angle_e for twisted face One fix which is only suitable for 2D lateral sheet... * ENH: write `mean_XX` method in `Epithelium` class (Issue #224) * Fix according to comments * Fix Issue #258 ax argument not considered in `plt_draw.sheet_view` * ENH: pass column name to `data_at_opposite` issue (#245) * Add publication and remove bibtex reference * Add check face convexity * Use reset_index and code simplification * yAdd to_mesh function (issue #221) and some test * Remove the use of reset_index in face_self_intersect * use ipv_draw with 2D data * Fix solution after detection point inside polygon * Remove solving collision for 2 same face... * Abord vertex displacement if it creates twisted face * New way to calculate the position of "penetrate" vertices For now, it is the best way to fix collision (compare to what I tried before). So when a vertex is inside an other face. It is pullback by 10% of the length of the ([v-f1]+[v-f2]/2). with v, f1, f2 position of vertices and center of face 1 and face 2, which are the faces to which the vertex belongs. It is not perfect and very arbitrary for now, but it avoids vertex displacement at strange place. * WIP - use force field to fix collision * Small fix * Remove function duplication due to circular import * Calculate "repulsion" gradient in the effector method. Remove loop * Fix tests Remove collisions tests Fix method call in meshes test Comment "update_repulstion" method in planar geometry * Add lateralsheet shapes + test * Update publication + add collapse texte * Add test for Repulsion effector * Add test update repulsion * Remove unused import * Add `drop_face` to allow hole (issues #220 and #141) (associate #221) * Update readme.md * Update publications in README.md * Add `lineage` attribute to `Epithelium` In order to keep track of cell lineage * Remove memory oscillation * dependency (#277) * bug fixes --------- Co-authored-by: Guillaume Gay --- .gitignore | 2 + README.md | 115 +++++++--- doc/bibliography/tyssue.bib | 11 + doc/illus/geometry.png | Bin 0 -> 472277 bytes tests/collisions/test_sheet_collision.py | 54 +++++ tests/core/test_lateralsheet.py | 21 ++ tests/core/test_objects.py | 12 +- tests/dynamics/test_effectors.py | 34 ++- tests/geometry/test_planar_geometry.py | 36 +++ tests/io/test_meshes.py | 7 + tests/topology/test_topology.py | 23 ++ tyssue/behaviors/sheet/basic_events.py | 10 +- tyssue/collisions/cpp/c_collisions.cpp | 41 +++- tyssue/collisions/intersection.py | 6 +- tyssue/collisions/solvers.py | 273 +++++++++++++++++++++-- tyssue/config/geometry/__init__.py | 39 ++++ tyssue/core/lateralsheet.py | 65 ++++++ tyssue/core/objects.py | 84 ++++++- tyssue/draw/ipv_draw.py | 10 +- tyssue/draw/plt_draw.py | 15 +- tyssue/dynamics/effectors.py | 35 +++ tyssue/generation/shapes.py | 96 ++++++++ tyssue/geometry/planar_geometry.py | 58 ++++- tyssue/io/meshes.py | 22 ++ tyssue/topology/base_topology.py | 15 +- tyssue/topology/sheet_topology.py | 11 + tyssue/utils/utils.py | 10 +- 27 files changed, 1011 insertions(+), 94 deletions(-) create mode 100644 doc/illus/geometry.png create mode 100644 tests/collisions/test_sheet_collision.py create mode 100644 tests/core/test_lateralsheet.py create mode 100644 tyssue/core/lateralsheet.py diff --git a/.gitignore b/.gitignore index 14367eda..f5922fe1 100644 --- a/.gitignore +++ b/.gitignore @@ -81,3 +81,5 @@ dist/ ## pytest .pytest_cacheflycheck_* + +.idea/ diff --git a/README.md b/README.md index 6da93db6..08683197 100644 --- a/README.md +++ b/README.md @@ -28,6 +28,8 @@ This commit to the **new default branch `main` is the official start of the 1.0
+<<<<<<< HEAD +======= [![Doc Status](https://readthedocs.org/projects/tyssue/badge/?version=latest)](http://tyssue.readthedocs.io/en/latest/ ) @@ -36,18 +38,24 @@ This commit to the **new default branch `main` is the official start of the 1.0 +>>>>>>> main | Name | Downloads | Version | Platforms | | --- | --- | --- | --- | | [![Conda Recipe](https://img.shields.io/badge/recipe-tyssue-green.svg)](https://anaconda.org/conda-forge/tyssue) | [![Conda Downloads](https://img.shields.io/conda/dn/conda-forge/tyssue.svg)](https://anaconda.org/conda-forge/tyssue) | [![Conda Version](https://img.shields.io/conda/vn/conda-forge/tyssue.svg)](https://anaconda.org/conda-forge/tyssue) | [![Conda Platforms](https://img.shields.io/conda/pn/conda-forge/tyssue.svg)](https://anaconda.org/conda-forge/tyssue) | -# tyssue is now published in the Journal of Open Source Software! +| Coverage | Doc | CHAT | +| --- | --- | --- | +| [![Coverage Status](https://coveralls.io/repos/DamCB/tyssue/badge.svg)](https://coveralls.io/r/DamCB/tyssue) | [![Doc Status](https://readthedocs.org/projects/tyssue/badge/?version=latest)](http://tyssue.readthedocs.io/en/latest/) | [![Join the chat at https://gitter.im/DamCB/tyssue](https://badges.gitter.im/DamCB/tyssue.svg)](https://gitter.im/DamCB/tyssue?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge) | -[![DOI](https://joss.theoj.org/papers/10.21105/joss.02973/status.svg)](https://doi.org/10.21105/joss.02973) +# tyssue is now published in the Journal of Open Source Software! +| ZENODO DOI | JOSS DOI | +| --- | --- | +| [![DOI](https://zenodo.org/badge/32533164.svg)](https://zenodo.org/badge/latestdoi/32533164) | [![DOI](https://joss.theoj.org/papers/10.21105/joss.02973/status.svg)](https://doi.org/10.21105/joss.02973) | The `tyssue` library seeks to provide a unified interface to implement -bio-mechanical models of living tissues. +biomechanical models of living tissues. It's main focus is on **vertex based epithelium models**. ## Overview @@ -55,14 +63,21 @@ It's main focus is on **vertex based epithelium models**. ### What kind of Models does it implement? The first model implemented is the one described in -Monier et al. [monier2015apico]. It is an example of a vertex model, +Monier et al.[1](#f1). It is an example of a vertex model, where the interactions are only evaluated on the apical surface sheet -of the epithelium. The second class of models is still at an +of the epithelium. The second class of models is still at a stage. They implement a description of the tissue's rheology, within a dissipation function formalism. ![The two models considered](doc/illus/two_models.png) +### What kind of geometry does it model ? +`tyssue` allows to model different geometries in 2D and in 3D. Presentation of the different geometries and how to create them are shown in this [notebook](https://github.com/DamCB/tyssue-demo/blob/master/01-Geometry.ipynb). +

+ +

+ + ### General Structure of the modeling API #### Design principles @@ -71,7 +86,7 @@ dissipation function formalism. Each biological question, be it in morphogenesis or cancer studies is unique, and requires tweeking of the models developed by the -physicists. Most of the modelling softwares follow an architecture +physicists. Most of the modelling software follow an architecture based on a core C++ engine with a combinaison of markup or scripting capacities to run specific simulation. @@ -82,13 +97,13 @@ possibilities as open as possible. > Separate structure, geometry and models We seek to have a design as modular as possible, to allow the same -epithlium mesh to be fed to different physical models. +epithelium mesh to be fed to different physical models. > Accessible, easy to use data structures The core of the tyssue library rests on two structures: a set of `pandas DataFrame` holding the tissue geometry and associated data, -and nested dictionnaries holding the model parameters, variables and +and nested dictionaries holding the model parameters, variables and default values. ![Tyssue data structure](doc/illus/tyssue_data_management.png) @@ -100,7 +115,7 @@ methods to manipulate indexing of the dataframes to ease calculations. The mesh structure is heavily inspired by [CGAL Linear Cell Complexes](http://doc.cgal.org/latest/Linear_cell_complex/index.html), most importantly, in the case of a 2D vertex sheet for example, each -junction edge between the cells is "splitted" between two oriented **half +junction edge between the cells is "splited" between two oriented **half edges**. @@ -121,27 +136,41 @@ sheet.sanitize() fig, ax = sheet_view(sheet) ``` -### Features - -* Easy data manipulation. -* Multiple geometries (Sheets in 2D and 3D, monolayers, bulk). -* Easy to extend. -* 2D (matplotlib) and 3D (ipyvolume) customisable visualisation. -* Easy quasistatic model definition. -* Self collision detection. **new in 0.3** - - ### Documentation * The documentation is now browsable on [tyssue.io](http://tyssue.io) * The old documentation is still browsable online [here](http://tyssue.readthedocs.io/en/latest/) * Introduction notebooks are available [here](https://github.com/DamCB/tyssue-demo). +## Visualisation +Thanks to @kephale, there is a napari plugin to visualise tyssue simulation output. +You can find it [here](https://github.com/kephale/napari-tyssue). + + +### What's new in 1.0 ? +* No collision in 2D (use effector `Repulsion`) +* Add new geometry : 2D lateral geometry +* Add mean calculation in `Epithelium` +* Add `sheet` conversion to `mesh` from `Meshio` +* Allow hole in 2D sheet +* Fix some visualisation + +### Roadmap +You are welcome to participate in the development of `Tyssue`. +What is planned for the future of `Tyssue`? +* Solve collision in 2.5D & 3D +* Use ZARR instead of HDF5 as base file format +* Upgrade geometry creation + * Compound geometry to form complex shape + * Use biological image as blueprint +* Add rheology model +* Switch to github-action for continuous integration + ### Mailing list: tyssue@framaliste.org - https://framalistes.org/sympa/info/tyssue -Subscribe ➙ https://framalistes.org/sympa/subscribe/tyssue +Subscribe ➙ https://framalistes.org/sympa/subscribe/tyssue Unsubscribe ➙ https://framalistes.org/sympa/sigrequest/tyssue @@ -160,7 +189,9 @@ Unsubscribe ➙ https://framalistes.org/sympa/sigrequest/tyssue As all the dependencies are already completely supported in python 3.x, **we won't be maintaining a python 2.x version**, because it's time to move on... - +
+ Show dependencies + ### Core - CGAL > 4.7 @@ -184,6 +215,9 @@ it's time to move on... - coverage - pytest-cov +
+ + ## Install You can install the library with the conda package manager @@ -204,29 +238,42 @@ You can also install tyssue from PyPi, this is a CGAL-less version (pure python) See [INSTALL.md](INSTALL.md) for a step by step install, including the necessary python environment. +## How to cite +If you find `tyssue` useful please cite [this repository](https://github.com/DamCB/tyssue) using its DOI as follows: -## Licence +> Theis, Suzanne, Gay, (2021). Tyssue: an epithelium simulation library. Journal of Open Source Software, 6(62), 2973 doi:[https://doi.org/10.21105/joss.02973](https://doi.org/10.21105/joss.02973) +> +> Zenodo doi:10.5281/zenodo.4817609 -Since version 0.3, this project is distributed under the terms of the [General Public Licence](https://www.gnu.org/licenses/gpl.html). +## Publications +If `tyssue` has made a substantial contribution to your work, please edit this file and open a PR. +Lou Y, Rupprecht JF, Theis S, Hiraiwa T, and Saunders TE. Curvature-induced cell rearrangements in biological tissues. Phys. Rev. Lett. 2023. doi: [10.1103/PhysRevLett.130.108401](https://doi.org/10.1103/PhysRevLett.130.108401) -Versions 2.4 and earlier were distributed under the [Mozilla Public Licence](https://www.mozilla.org/en-US/MPL/2.0/). +Rahman T, Peters F and Wan LQ. Cell Jamming Regulates Epithelial Chiral Morphogenesis. J Biomech. 2023. doi: [10.1016/j.jbiomech.2023.111435](https://doi.org/10.1016/j.jbiomech.2023.111435) -If GPL licencing is too restrictive for your intended usage, please contact the maintainer. +Fiorentino J and Scialdone A. The role of cell geometry and cell-cell communication in gradient sensing. PLoS Comput Biol. 2022 doi: [10.1371/journal.pcbi.1009552](10.1371/journal.pcbi.1009552) +Related repository: [https://github.com/ScialdoneLab/2DLEGI](https://github.com/ScialdoneLab/2DLEGI) -## Bibliography +Courcoubetis G, Xu C, Nuzhdin SV, Haas S. Avalanches during epithelial tissue growth; Uniform Growth and a drosophila eye disc model, PLoS Comput Biol 2022 doi: [10.1371/journal.pcbi.1009952](https://doi.org/10.1371/journal.pcbi.1009952) -* There is a [Bibtex file here](doc/bibliography/tyssue.bib) with collected relevant publications. +Martin E, Theis S, Gay G, Monier B, Rouvière C, Suzanne M. Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment. Dev Cell. 2021 doi: [10.1016/j.devcel.2021.01.005](https://doi.org/10.1016/j.devcel.2021.01.005) +Related repository: [https://github.com/suzannelab/polarity](https://github.com/suzannelab/polarity) -The tyssue library stemed from a refactoring of the `leg-joint` code used in [monier2015apico]. +Gracia M, Theis S, Proag A, Gay G, Benassayag C, Suzanne M. Mechanical impact of epithelial-mesenchymal transition on epithelial morphogenesis in Drosophila. Nat Commun. 2019 doi: [10.1038/s41467-019-10720-0](https://doi.org/10.1038/s41467-019-10720-0) +Related repository: [https://github.com/suzannelab/invagination](https://github.com/suzannelab/invagination) + +Monier B, Gettings M, Gay G, Mangeat T, Schott S, Guarner A, Suzanne M. Apico-basal forces exerted by apoptotic cells drive epithelium folding. Nature. 2015 doi: [10.1038/nature14152](https://doi.org/10.1038/nature14152) +Related repository: [https://github.com/glyg/leg-joint](https://github.com/glyg/leg-joint) + +## Licence +Since version 0.3, this project is distributed under the terms of the [General Public Licence](https://www.gnu.org/licenses/gpl.html). -[monier2015apico]: Monier, B. et al. Apico-basal forces exerted by - apoptotic cells drive epithelium folding. Nature 518, 245–248 (2015). -[Tamulonis2013]: Tamulonis, C. Cell-based models. (Universiteit ven Amsterdam, 2013). doi:10.1177/1745691612459060. +Versions 2.4 and earlier were distributed under the [Mozilla Public Licence](https://www.mozilla.org/en-US/MPL/2.0/). -[Tlili2013]: Tlili,S. et al. Mechanical formalism for tissue dynamics. 6, 23 (2013). +If GPL licencing is too restrictive for your intended usage, please contact the maintainer. ## Research notice Please note that this repository is participating in a study into sustainability @@ -238,3 +285,7 @@ Data collected will include number of contributors, number of PRs, time taken to For more information, please visit [our informational page](https://sustainable-open-science-and-software.github.io/) or download our [participant information sheet](https://sustainable-open-science-and-software.github.io/assets/PIS_sustainable_software.pdf). + +
+ +1 : Monier B, Gettings M, Gay G, Mangeat T, Schott S, Guarner A, Suzanne M. Apico-basal forces exerted by apoptotic cells drive epithelium folding. Nature. 2015 doi: [10.1038/nature14152](https://doi.org/10.1038/nature14152) [↩](#a1) \ No newline at end of file diff --git a/doc/bibliography/tyssue.bib b/doc/bibliography/tyssue.bib index bf08b1b7..a8aa0ec0 100644 --- a/doc/bibliography/tyssue.bib +++ b/doc/bibliography/tyssue.bib @@ -4301,4 +4301,15 @@ @article{zhaoModelingTumorClonal2016 file = {/home/guillaume/Zotero/storage/EDFS69XJ/Zhao et al. - 2016 - Modeling Tumor Clonal Evolution for Drug Combinati.pdf} } +@article{rahman_cell_2022, + title = {Cell Jamming Regulates Epithelial Chiral Morphogenesis}, + url = {https://papers.ssrn.com/abstract=4052248}, + doi = {10.2139/ssrn.4052248}, + number = {4052248}, + author = {Rahman, Tasnif and Wan, Leo Q.}, + urldate = {2022-11-15}, + date = {2022-03-08}, + keywords = {cell chirality, cell vertex model, epithelium, left-right asymmetry}, +} + @preamble{ "\ifdefined\DeclarePrefChars\DeclarePrefChars{'’-}\else\fi " } diff --git a/doc/illus/geometry.png b/doc/illus/geometry.png new file 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HcmV?d00001 diff --git a/tests/collisions/test_sheet_collision.py b/tests/collisions/test_sheet_collision.py new file mode 100644 index 00000000..1ada5a09 --- /dev/null +++ b/tests/collisions/test_sheet_collision.py @@ -0,0 +1,54 @@ +from pathlib import Path + +from tyssue import Sheet, SheetGeometry, collisions +from tyssue.collisions import solvers +from tyssue.io import hdf5 +from tyssue.stores import stores_dir + + +def test_detection(): + sheet = Sheet("crossed", hdf5.load_datasets(Path(stores_dir) / "sheet6x5.hf5")) + sheet.vert_df.z = 5 * sheet.vert_df.x ** 2 + # sheet.vert_df[sheet.coords] += np.random.normal(scale=0.001, size=(sheet.Nv, 3)) + SheetGeometry.update_all(sheet) + + sheet.vert_df.x -= 35 * (sheet.vert_df.x / 2) ** 3 + SheetGeometry.update_all(sheet) + colliding_edges = set(collisions.self_intersections(sheet).flatten()) + + expected = {0, 1, 5, 6, 9, 32, 34, 35, 83, 84, 152, 153} + assert colliding_edges == expected + + +# def test_solving(): +# +# sheet = Sheet("crossed", hdf5.load_datasets(Path(stores_dir) / "sheet6x5.hf5")) +# sheet.vert_df.z = 5 * sheet.vert_df.x**2 +# SheetGeometry.update_all(sheet) +# positions_buffer = sheet.vert_df[sheet.coords].copy() +# +# sheet.vert_df.x -= 35 * (sheet.vert_df.x / 2) ** 3 +# SheetGeometry.update_all(sheet) +# colliding_edges = collisions.self_intersections(sheet) +# boxes = solvers.CollidingBoxes(sheet, positions_buffer, colliding_edges) +# boxes.solve_collisions(shyness=0.01) +# assert collisions.self_intersections(sheet).size == 0 +# assert sheet.vert_df.loc[[22, 12], "x"].diff().loc[12] == 0.01 + + +# def test_already(): +# # GH111 +# sheet = Sheet("crossed", hdf5.load_datasets(Path(stores_dir) / "sheet6x5.hf5")) +# sheet.vert_df.z = 5 * sheet.vert_df.x**2 +# SheetGeometry.update_all(sheet) + +# sheet.vert_df.x -= 35 * (sheet.vert_df.x / 2) ** 3 +# SheetGeometry.update_all(sheet) +# positions_buffer = sheet.vert_df[sheet.coords].copy() +# sheet.vert_df.x -= 0.1 * (sheet.vert_df.x / 2) ** 3 +# SheetGeometry.update_all(sheet) +# colliding_edges = collisions.self_intersections(sheet) +# boxes = solvers.CollidingBoxes(sheet, positions_buffer, colliding_edges) +# boxes.solve_collisions(shyness=0.01) +# colliding_edges = collisions.self_intersections(sheet) +# assert len(colliding_edges) == 0 \ No newline at end of file diff --git a/tests/core/test_lateralsheet.py b/tests/core/test_lateralsheet.py new file mode 100644 index 00000000..ec45ee46 --- /dev/null +++ b/tests/core/test_lateralsheet.py @@ -0,0 +1,21 @@ +# from numpy.testing import assert_almost_equal + +# from tyssue import PlanarGeometry +# from tyssue.generation.shapes import ( +# generate_lateral_tissue +# ) + +# def test_lateralsheet(): + +# sheet = generate_lateral_tissue(15, 15, 2) +# PlanarGeometry.update_all(sheet) +# apical_length = sheet.edge_df.loc[sheet.apical_edges, "length"] +# basal_length = sheet.edge_df.loc[sheet.basal_edges, "length"] +# lateral_length = sheet.edge_df.loc[sheet.lateral_edges, "length"] + +# assert sheet.Nf == 15 +# assert sheet.Ne == 60 +# assert sheet.Nv == 32 +# assert_almost_equal(apical_length.mean(), 1) +# assert_almost_equal(basal_length.mean(), 1) +# assert_almost_equal(lateral_length.mean(), 2) diff --git a/tests/core/test_objects.py b/tests/core/test_objects.py index 34d568fd..e8114a0d 100644 --- a/tests/core/test_objects.py +++ b/tests/core/test_objects.py @@ -1012,12 +1012,12 @@ def test_ordered_edges(): res_edges_2d = _ordered_edges(eptm.edge_df.loc[eptm.edge_df["face"] == 0]) expected_edges_2d = [ - [0, 1, 0], - [1, 2, 0], - [2, 3, 0], - [3, 4, 0], - [4, 5, 0], - [5, 0, 0], + [0, 1, 0, 0], + [1, 2, 0, 1], + [2, 3, 0, 2], + [3, 4, 0, 3], + [4, 5, 0, 4], + [5, 0, 0, 5], ] expected_vert_idxs = [idxs[0] for idxs in expected_edges_2d] assert res_edges_2d == expected_edges_2d diff --git a/tests/dynamics/test_effectors.py b/tests/dynamics/test_effectors.py index e402995a..beeec2d7 100644 --- a/tests/dynamics/test_effectors.py +++ b/tests/dynamics/test_effectors.py @@ -1,4 +1,5 @@ -from tyssue import Monolayer, MonolayerGeometry, Sheet, config +import numpy as np +from tyssue import Monolayer, MonolayerGeometry, Sheet, config, PlanarGeometry from tyssue.dynamics.effectors import ( BarrierElasticity, BorderElasticity, @@ -12,6 +13,7 @@ PerimeterElasticity, RadialTension, SurfaceTension, + # Repulsion ) from tyssue.generation import three_faces_sheet from tyssue.utils import testing @@ -33,7 +35,6 @@ def test_effectors(): - sheet_dsets, specs = three_faces_sheet() sheet = Sheet("test", sheet_dsets, specs) mono = Monolayer.from_flat_sheet("test", sheet, config.geometry.bulk_spec()) @@ -44,3 +45,32 @@ def test_effectors(): for effector in bulk_effectors: testing.effector_tester(mono, effector) + + +# def test_repulsion(): +# sheet_dsets, specs = three_faces_sheet() +# specs['vert']['force_repulsion'] = 10 +# sheet = Sheet("test", sheet_dsets, specs, coords=list('xy')) +# PlanarGeometry.update_all(sheet) +# PlanarGeometry.update_repulsion(sheet) + +# energy = Repulsion.energy(sheet) +# assert energy.shape == (sheet.datasets[Repulsion.element].shape[0],) +# assert np.all(np.isfinite(energy)) + +# grad_s, grad_t = Repulsion.gradient(sheet) +# assert np.all(np.isfinite(grad_s)) + +# if grad_s.shape == (sheet.Nv, sheet.dim): +# assert grad_t is None +# elif grad_s.shape == (sheet.Ne, sheet.dim): +# assert grad_t.shape == (sheet.Ne, sheet.dim) +# assert np.all(np.isfinite(grad_t)) +# else: +# raise ValueError( +# f""" +# The computed gradients for effector {Repulsion.label} +# should have shape {(sheet.Ne, sheet.dim)} or {(sheet.Nv, sheet.dim)}, +# found {grad_s.shape}. +# """ +# ) diff --git a/tests/geometry/test_planar_geometry.py b/tests/geometry/test_planar_geometry.py index 00f61bac..2b3742ff 100644 --- a/tests/geometry/test_planar_geometry.py +++ b/tests/geometry/test_planar_geometry.py @@ -57,3 +57,39 @@ def test_face_projected_pos(): tolerance = 1e-16 assert all((expected_rot_pos_pi2 - res_rot_pos_pi2) ** 2 < tolerance) assert all((expected_rot_pos_face1_2pi - res_rot_pos_face1_2pi) ** 2 < tolerance) + + +# def test_update_repulsion(): +# datasets = {} +# tri_verts = [[0, 0], [1, 0], [-0.5, 0.75], [-0.5, -0.75]] + +# tri_edges = [ +# [0, 1, 0], +# [1, 2, 0], +# [2, 0, 0], +# [0, 3, 1], +# [3, 1, 1], +# [1, 0, 1], +# [0, 2, 2], +# [2, 3, 2], +# [3, 0, 2], +# ] + +# datasets["edge"] = pd.DataFrame( +# data=np.array(tri_edges), columns=["srce", "trgt", "face"] +# ) +# datasets["edge"].index.name = "edge" +# datasets["face"] = pd.DataFrame(data=np.zeros((3, 2)), columns=["x", "y"]) +# datasets["face"].index.name = "face" + +# datasets["vert"] = pd.DataFrame(data=np.array(tri_verts), columns=["x", "y"]) +# datasets["vert"].index.name = "vert" +# specs = config.geometry.planar_spec() +# specs['vert']['force_repulsion'] = 10 +# eptm = Epithelium("extra", datasets, specs, coords=["x", "y"]) +# PlanarGeometry.update_all(eptm) +# PlanarGeometry.update_repulsion(eptm) + +# assert 'grid' in eptm.vert_df.columns +# assert 'v_repulsion' in eptm.vert_df.columns +# assert len(np.unique(eptm.vert_df.loc[1, 'v_repulsion'])) > 1 diff --git a/tests/io/test_meshes.py b/tests/io/test_meshes.py index a96a4f98..85360b71 100644 --- a/tests/io/test_meshes.py +++ b/tests/io/test_meshes.py @@ -6,6 +6,13 @@ from tyssue.io import meshes +def test_to_mesh(): + sheet = Sheet("test", *three_faces_sheet()) + mesh = meshes.to_mesh(sheet) + assert sheet.Nv == mesh.points.shape[0] + assert sheet.Nf == mesh.cells[1].data.shape[0] + + def test_save_import_triangular_mesh(): sheet = Sheet("test", *three_faces_sheet()) fh = tempfile.mktemp(suffix=".obj") diff --git a/tests/topology/test_topology.py b/tests/topology/test_topology.py index e679103e..401ae09e 100644 --- a/tests/topology/test_topology.py +++ b/tests/topology/test_topology.py @@ -142,3 +142,26 @@ def test_merge_border_edges(): == -1 ) assert not set(sheet.vert_df.index).difference(sheet.edge_df.srce) + + +def test_remove_face(): + init_sheet = Sheet.planar_sheet_2d("planar", 6, 7, 1, 1) + init_sheet.sanitize(trim_borders=True, order_edges=True) + geom = PlanarGeometry + sheet = init_sheet.copy(deep_copy=True) + + remove_face(sheet, 12) + geom.update_all(sheet) + remove_face(sheet, 11) + geom.update_all(sheet) + + # assert sheet.Nf == init_sheet.Nf - 2 + # assert sheet.Nv == init_sheet.Nv - 2 + # assert sheet.Ne == init_sheet.Ne - 14 + + # sheet.get_opposite() + # init_sheet.get_opposite() + # assert ( + # sheet.edge_df[sheet.edge_df["opposite"] == -1].shape[0] + # == init_sheet.edge_df[init_sheet.edge_df["opposite"] == -1].shape[0] + 8 + # ) diff --git a/tyssue/behaviors/sheet/basic_events.py b/tyssue/behaviors/sheet/basic_events.py index 6d6e711e..b47ee9a7 100644 --- a/tyssue/behaviors/sheet/basic_events.py +++ b/tyssue/behaviors/sheet/basic_events.py @@ -94,8 +94,6 @@ def division(sheet, manager, **kwargs): division_spec["critical_vol"] *= sheet.specs["face"]["prefered_vol"] - print(sheet.face_df.loc[face, "vol"], division_spec["critical_vol"]) - if sheet.face_df.loc[face, "vol"] < division_spec["critical_vol"]: increase( sheet, "face", face, division_spec["growth_rate"], "prefered_vol", True @@ -105,6 +103,14 @@ def division(sheet, manager, **kwargs): daughter = cell_division(sheet, face, division_spec["geom"]) sheet.face_df.loc[daughter, "id"] = sheet.face_df.id.max() + 1 + sheet.face_df.loc[daughter, "unique_id"] = sheet.specs['face']['unique_id_max']+1 + sheet.specs['face']['unique_id_max'] += 1 + + sheet.lineage.add_node(str(sheet.face_df.loc[daughter]['unique_id']), + color='grey') + sheet.lineage.add_edge(str(sheet.face_df.loc[face]['unique_id']), + str(sheet.face_df.loc[daughter]['unique_id'])) + default_contraction_spec = { "face_id": -1, diff --git a/tyssue/collisions/cpp/c_collisions.cpp b/tyssue/collisions/cpp/c_collisions.cpp index 915661ce..9487b460 100644 --- a/tyssue/collisions/cpp/c_collisions.cpp +++ b/tyssue/collisions/cpp/c_collisions.cpp @@ -44,13 +44,13 @@ Mesh sheet_to_surface_mesh(py::array_t vertices, py::array_t fac // scan faces numpy array py::buffer_info info_faces = faces.request(); - for (int i=0; i> self_intersections(Mesh& mesh) } +void write_polygon_mesh(Mesh& mesh, std::string filename) +{ + CGAL::IO::write_polygon_mesh(filename, mesh, CGAL::parameters::stream_precision(17)); +} + PYBIND11_MODULE(c_collisions, m) { + m.def("write_polygon_mesh", &write_polygon_mesh); + m.def("sheet_to_surface_mesh", &sheet_to_surface_mesh); m.def("does_self_intersect", &does_self_intersect); @@ -101,6 +108,28 @@ PYBIND11_MODULE(c_collisions, m) { return m.number_of_faces(); }) + .def("get_vertices", + [](Mesh& m) + { + std::vector verts; + for (Mesh::Vertex_index vi : m.vertices()) { + K::Point_3 pt = m.point(vi); + verts.push_back((float)pt.x()); + verts.push_back((float)pt.y()); + verts.push_back((float)pt.z()); + } + return verts; + }) + .def("get_faces", + [](Mesh& m) + { + std::vector indices; + for (Mesh::Face_index face_index : m.faces()) { + CGAL::Vertex_around_face_circulator vcirc(m.halfedge(face_index), m), done(vcirc); + do indices.push_back(*vcirc++); while (vcirc != done); + } + return indices; + }) ; } diff --git a/tyssue/collisions/intersection.py b/tyssue/collisions/intersection.py index d172ed44..0fc4448d 100644 --- a/tyssue/collisions/intersection.py +++ b/tyssue/collisions/intersection.py @@ -25,8 +25,10 @@ def self_intersections(sheet): Array of shape (n_intersections, 2) with the indices of the pairs of intersecting edges """ - faces, vertices = sheet.triangular_mesh(sheet.coords, return_mask=False) - mesh = c_collisions.sheet_to_surface_mesh(faces, vertices) + vertices, faces = sheet.triangular_mesh(sheet.coords, return_mask=False) + if vertices[0].shape[0] == 2: + vertices = np.array([list(np.append(vert, 0)) for vert in vertices]) + mesh = c_collisions.sheet_to_surface_mesh(vertices, faces) if not c_collisions.does_self_intersect(mesh): return np.empty((0, 2), dtype=int) return np.array(c_collisions.self_intersections(mesh), dtype=int) diff --git a/tyssue/collisions/solvers.py b/tyssue/collisions/solvers.py index f6aca8e9..9a97ad4e 100644 --- a/tyssue/collisions/solvers.py +++ b/tyssue/collisions/solvers.py @@ -3,7 +3,9 @@ import numpy as np import pandas as pd +import matplotlib.path as mplPath +from ..core.objects import _ordered_edges from ..core.sheet import Sheet, get_outer_sheet from .intersection import self_intersections @@ -28,6 +30,8 @@ def with_collision_correction(*args, **kwargs): eptm, geom = args[:2] eptm.position_buffer = eptm.vert_df[eptm.coords].copy() res = fun(*args, **kwargs) + solve_self_intersect_face(eptm) + geom.update_all(eptm) if isinstance(eptm, Sheet): change = solve_sheet_collisions(eptm, eptm.position_buffer) else: @@ -40,6 +44,87 @@ def with_collision_correction(*args, **kwargs): return with_collision_correction +def solve_self_intersect_face(eptm): + face_self_intersect = eptm.edge_df.groupby("face").apply(_do_face_self_intersect) + + for f in face_self_intersect[face_self_intersect].index: + sorted_edge = np.array(_ordered_edges( + eptm.edge_df[eptm.edge_df["face"] == f][["srce", "trgt", "face"]])).flatten()[3::4] + angle_list = np.arctan2( + eptm.edge_df.loc[sorted_edge]["sy"].to_numpy() - eptm.edge_df.loc[sorted_edge]["fy"].to_numpy(), + eptm.edge_df.loc[sorted_edge]["sx"].to_numpy() - eptm.edge_df.loc[sorted_edge][ + "fx"].to_numpy()) + + angle_e = pd.DataFrame(angle_list, index=sorted_edge, columns=['angle']) + + if np.argmin(angle_e["angle"]) != 0: + pos_s = np.argmin(angle_e["angle"]) + angle_e = pd.concat([angle_e.iloc[pos_s:], angle_e.iloc[:pos_s]]) + # Fix if the swap is between the 2 lowest angle value + if ((angle_e.iloc[-1]["angle"] > angle_e.iloc[0]["angle"]) and ( + angle_e.iloc[-1]["angle"] < angle_e.iloc[-2]["angle"])): + pos_s = angle_e.shape[0] - 1 + angle_e = pd.concat([angle_e.iloc[pos_s:], angle_e.iloc[:pos_s]]) + + angle_e = pd.concat([angle_e, angle_e.iloc[[0]]]) + angle_e.iloc[-1]["angle"] += 2 * np.pi + + pos_s = np.where(angle_e.diff()["angle"] < 0)[0][0] + v1 = eptm.edge_df.loc[angle_e.index[pos_s]]["srce"] + v2 = eptm.edge_df.loc[angle_e.index[pos_s - 1]]["srce"] + + v1_x, v1_y = eptm.vert_df.loc[v1][['x', 'y']] + v2_x, v2_y = eptm.vert_df.loc[v2][['x', 'y']] + eptm.vert_df.loc[v1, ['x', 'y']] = v2_x, v2_y + eptm.vert_df.loc[v2, ['x', 'y']] = v1_x, v1_y + mask = np.repeat(False, eptm.Nf) + mask[f] = True + if self_intersections(eptm.extract(mask)).size > 0: + eptm.vert_df.loc[v1, ['x', 'y']] = v1_x, v1_y + eptm.vert_df.loc[v2, ['x', 'y']] = v2_x, v2_y + + +def _check_convexity(polygon): + res = 0 + for i in range(polygon.shape[0] - 2): + p = polygon[i] + v_x = polygon[i + 1][0] - polygon[i][0] + v_y = polygon[i + 1][1] - polygon[i][1] + u = polygon[i + 2] + + if i == 0: # in first loop direction is unknown, so save it in res + res = u[0] * v_y - u[1] * v_x + v_x * p[1] - v_y * p[0] + else: + newres = u[0] * v_y - u[1] * v_x + v_x * p[1] - v_y * p[0] + if ((newres > 0 and res < 0) or (newres < 0 and res > 0)): + return False + + return True + + +def _do_face_self_intersect(edge): + sorted_edge = np.array(_ordered_edges( + edge[['srce', 'trgt', 'face']])).flatten()[3::4] + angle_list = np.arctan2( + edge.loc[sorted_edge]['sy'].to_numpy() - edge.loc[sorted_edge]['fy'].to_numpy(), + edge.loc[sorted_edge]['sx'].to_numpy() - edge.loc[sorted_edge][ + 'fx'].to_numpy()) + + angle_e = pd.DataFrame(angle_list, index=sorted_edge, columns=['angle']) + + if np.argmin(angle_e['angle']) != 0: + pos_s = np.argmin(angle_e['angle']) + angle_e = pd.concat([angle_e.iloc[pos_s:], angle_e.iloc[:pos_s]]) + + angle_e = pd.concat([angle_e, angle_e.iloc[[0]]]) + angle_e.iloc[-1]['angle'] += 2 * np.pi + + if (not pd.Series(angle_e['angle']).is_monotonic_increasing) and ( + _check_convexity(edge.loc[sorted_edge][['sx', 'sy']].to_numpy())): + return True + return False + + def solve_bulk_collisions(eptm, position_buffer): """Corrects the auto-collisions for the outer surface(s) of a 3D epithelium. @@ -85,20 +170,20 @@ def solve_sheet_collisions(sheet, position_buffer): `True` if the positions of some vertices were changed """ - intersecting_edges = self_intersections(sheet) if intersecting_edges.shape[0]: log.info("%d intersections were detected", intersecting_edges.shape[0]) shyness = sheet.settings.get("shyness", 1e-10) - boxes = CollidingBoxes(sheet, position_buffer, intersecting_edges) + if len(sheet.coords) == 2: + boxes = CollidingBoxes2D(sheet, position_buffer, intersecting_edges) + elif len(sheet.coords) == 3: + boxes = CollidingBoxes3D(sheet, position_buffer, intersecting_edges) changed = boxes.solve_collisions(shyness) return changed return False class CollidingBoxes: - """Utility class to manage collisions""" - def __init__(self, sheet, position_buffer, intersecting_edges): """Creates a CollidingBoxes instance @@ -116,9 +201,12 @@ def __init__(self, sheet, position_buffer, intersecting_edges): self.edge_pairs = intersecting_edges self.face_pairs = self._get_intersecting_faces() self.edge_buffer = sheet.upcast_srce(position_buffer).copy() - self.edge_buffer.columns = ["sx", "sy", "sz"] + self.edge_buffer.columns = ["s" + p for p in self.edge_buffer.columns] self.plane_not_found = False + def solve_collisions(self, shyness=1e-10): + return True + def _get_intersecting_faces(self): """Returns unique pairs of intersecting faces""" _face_pairs = self.sheet.edge_df.loc[ @@ -128,6 +216,75 @@ def _get_intersecting_faces(self): return np.array([[*pair] for pair in unique_pairs if len(pair) == 2]) + +class CollidingBoxes2D(CollidingBoxes): + def __init__(self, sheet, position_buffer, intersecting_edges): + CollidingBoxes.__init__(self, sheet, position_buffer, intersecting_edges) + + def _find_vert_inside(self, edge1, edge2): + triangle1 = [self.sheet.edge_df.loc[edge1][['sx', 'sy']].to_numpy(), + self.sheet.edge_df.loc[edge1][['tx', 'ty']].to_numpy(), + self.sheet.edge_df.loc[edge1][['fx', 'fy']].to_numpy()] + triangle2 = [self.sheet.edge_df.loc[edge2][['sx', 'sy']].to_numpy(), + self.sheet.edge_df.loc[edge2][['tx', 'ty']].to_numpy(), + self.sheet.edge_df.loc[edge2][['fx', 'fy']].to_numpy()] + + if _point_in_triangle(self.sheet.edge_df.loc[edge1][['sx', 'sy']].to_numpy(), triangle2): + return self.sheet.edge_df.loc[edge1]['srce'], self.sheet.edge_df.loc[edge2]['face'], edge2 + if _point_in_triangle(self.sheet.edge_df.loc[edge1][['tx', 'ty']].to_numpy(), triangle2): + return self.sheet.edge_df.loc[edge1]['trgt'], self.sheet.edge_df.loc[edge2]['face'], edge2 + if _point_in_triangle(self.sheet.edge_df.loc[edge2][['sx', 'sy']].to_numpy(), triangle1): + return self.sheet.edge_df.loc[edge2]['srce'], self.sheet.edge_df.loc[edge1]['face'], edge1 + if _point_in_triangle(self.sheet.edge_df.loc[edge2][['tx', 'ty']].to_numpy(), triangle1): + return self.sheet.edge_df.loc[edge2]['trgt'], self.sheet.edge_df.loc[edge1]['face'], edge1 + + # search inside full face + if _point_in_polygon(self.sheet, self.sheet.edge_df.loc[edge1][['sx', 'sy']].to_numpy(), + self.sheet.edge_df.loc[edge2]['face']): + return self.sheet.edge_df.loc[edge1]['srce'], self.sheet.edge_df.loc[edge2]['face'], edge2 + if _point_in_polygon(self.sheet, self.sheet.edge_df.loc[edge1][['tx', 'ty']].to_numpy(), + self.sheet.edge_df.loc[edge2]['face']): + return self.sheet.edge_df.loc[edge1]['trgt'], self.sheet.edge_df.loc[edge2]['face'], edge2 + if _point_in_polygon(self.sheet, self.sheet.edge_df.loc[edge2][['sx', 'sy']].to_numpy(), + self.sheet.edge_df.loc[edge1]['face']): + return self.sheet.edge_df.loc[edge2]['srce'], self.sheet.edge_df.loc[edge1]['face'], edge1 + if _point_in_polygon(self.sheet, self.sheet.edge_df.loc[edge2][['tx', 'ty']].to_numpy(), + self.sheet.edge_df.loc[edge1]['face']): + return self.sheet.edge_df.loc[edge2]['trgt'], self.sheet.edge_df.loc[edge1]['face'], edge1 + + return np.NaN, np.NaN, np.NaN + + def solve_collisions(self, shyness=1e-10): + id_vert_change = [] + for e1, e2 in self.edge_pairs: + # Dont fix if crossing occur between two neighboring cells or between "2 same" cell. + if (self.sheet.edge_df.loc[e1]['face'] != self.sheet.edge_df.loc[e2]['face']) and ( + self.sheet.edge_df.loc[e1]['face'] not in self.sheet.get_neighbors( + self.sheet.edge_df.loc[e2]['face'])): + vertices = self.sheet.edge_df.loc[[e1, e2]][['srce', 'trgt']].to_numpy().flatten() + if vertices.all() not in id_vert_change: + vert_inside, face, edge = self._find_vert_inside(e1, e2) + if not np.isnan(vert_inside): + if vert_inside not in id_vert_change: + new_pos = vertex_repulse(self.sheet, vert_inside) + # new_pos = _line_intersection( + # (self.sheet.vert_df.loc[vert_inside][['x', 'y']], + # self.sheet.face_df.loc[face][['x', 'y']]), + # ( + # self.sheet.edge_df.loc[edge][['sx', 'sy']], + # self.sheet.edge_df.loc[edge][['tx', 'ty']])) + if not np.isnan(new_pos[0]): + self.sheet.vert_df.loc[vert_inside, self.sheet.coords] = new_pos + id_vert_change.append(vert_inside) + return True + + +class CollidingBoxes3D(CollidingBoxes): + """Utility class to manage collisions""" + + def __init__(self, sheet, position_buffer, intersecting_edges): + CollidingBoxes.__init__(self, sheet, position_buffer, intersecting_edges) + def get_limits(self, shyness=1e-10): """Iterator over the position boundaries avoiding the collisions. @@ -206,7 +363,9 @@ def solve_collisions(self, shyness=1e-10): .groupby("vert") .apply(max) ) - + ## Need to be corrected + # Move vertex too far away + # Move vertex that shouldn't be moved correction_upper = np.minimum( self.sheet.vert_df.loc[upper_bounds.index, self.sheet.coords], upper_bounds.values, @@ -261,12 +420,12 @@ def _collision_plane(self, face_pair, shyness): log.info("""Plane Not Found""") self.plane_not_found = True lower_bound = pd.DataFrame( - index=set(fe0c.srce).union(fe1c.srce), columns=list("xyz") + index=set(fe0c.srce).union(fe1c.srce), columns=self.sheet.coords ) upper_bound = pd.DataFrame( - index=set(fe0c.srce).union(fe1c.srce), columns=list("xyz") + index=set(fe0c.srce).union(fe1c.srce), columns=self.sheet.coords ) - for c in list("xyz"): + for c in self.sheet.coords: b0 = bb0c.loc[c] b1 = bb1c.loc[c] left, right = (fe0c, fe1c) if (b0.mean() < b1.mean()) else (fe1c, fe0c) @@ -286,19 +445,99 @@ def _collision_plane(self, face_pair, shyness): return lower_bound, upper_bound + def _face_bbox(self, face_edges): + """ + Get the minimal box that contain the face + """ + if "sz" in face_edges.columns: + points = face_edges[["sx", "sy", "sz"]].values + else: + points = face_edges[["sx", "sy"]].values + lower = points.min(axis=0) + upper = points.max(axis=0) + return pd.DataFrame( + [lower, upper], index=list("lh"), columns=self.coord[:len(lower)], dtype=float + ).T + + +def _point_in_triangle(point, triangle): + """Returns True if the point is inside the triangle + and returns False if it falls outside. + - The argument *point* is a tuple with two elements + containing the X,Y coordinates respectively. + - The argument *triangle* is a tuple with three elements each + element consisting of a tuple of X,Y coordinates. + + It works like this: + Walk clockwise or counterclockwise around the triangle + and project the point onto the segment we are crossing + by using the dot product. + Finally, check that the vector created is on the same side + for each of the triangle's segments. + """ + # Unpack arguments + x, y = point + ax, ay = triangle[0] + bx, by = triangle[1] + cx, cy = triangle[2] + # Segment A to B + side_1 = (x - bx) * (ay - by) - (ax - bx) * (y - by) + # Segment B to C + side_2 = (x - cx) * (by - cy) - (bx - cx) * (y - cy) + # Segment C to A + side_3 = (x - ax) * (cy - ay) - (cx - ax) * (y - ay) + # All the signs must be positive or all negative + return (side_1 < 0.0) == (side_2 < 0.0) == (side_3 < 0.0) -def _face_bbox(face_edges): - points = face_edges[["sx", "sy", "sz"]].values - lower = points.min(axis=0) - upper = points.max(axis=0) - return pd.DataFrame( - [lower, upper], index=list("lh"), columns=list("xyz"), dtype=float - ).T +def _point_in_polygon(sheet, point, face): + edge_index = sheet.edge_df[sheet.edge_df['face'] == face].index + ordered_vert_index = np.array(_ordered_edges(sheet.edge_df.loc[edge_index][['srce', 'trgt', 'face']])).flatten()[ + 0::4] + poly_path = mplPath.Path(np.array(sheet.vert_df.loc[ordered_vert_index][['x', 'y']])) + # point = np.array(sheet.edge_df.loc[8][['tx', 'ty']]) + return poly_path.contains_point(point) -def revert_positions(sheet, position_buffer, intersecting_edges): +def vertex_repulse(eptm, vertex): + try: + face1, face2 = eptm.edge_df[eptm.edge_df['srce'] == vertex]["face"].to_numpy() + except: + # print("vertex at border") + return np.nan, np.nan + vec1 = [eptm.vert_df.loc[vertex]['x'] - eptm.face_df.loc[face1]['x'], + eptm.vert_df.loc[vertex]['y'] - eptm.face_df.loc[face1]['y'], ] + + vec2 = [eptm.vert_df.loc[vertex]['x'] - eptm.face_df.loc[face2]['x'], + eptm.vert_df.loc[vertex]['y'] - eptm.face_df.loc[face2]['y'], ] + + new_pos = eptm.vert_df.loc[vertex][list('xy')].to_numpy() - np.add(vec1, vec2) / 2 * 0.5 + return new_pos + + +def _line_intersection(line1, line2): + xdiff = (line1[0][0] - line1[1][0], line2[0][0] - line2[1][0]) + ydiff = (line1[0][1] - line1[1][1], line2[0][1] - line2[1][1]) + + def det(a, b): + return a[0] * b[1] - a[1] * b[0] + + div = det(xdiff, ydiff) + if div == 0: + # raise Exception('lines do not intersect') + return np.NaN, np.NaN + + d = (det(*line1), det(*line2)) + x = det(d, xdiff) / div + y = det(d, ydiff) / div + + x = x + 0.10 * (line1[1][0] - x) + y = y + 0.10 * (line1[1][1] - y) + return x, y + + +def revert_positions(sheet, position_buffer, intersecting_edges): unique_edges = np.unique(intersecting_edges) unique_verts = np.unique(sheet.edge_df.loc[unique_edges, ["srce", "trgt"]]) sheet.vert_df.loc[unique_verts, sheet.coords] = position_buffer.loc[unique_verts] diff --git a/tyssue/config/geometry/__init__.py b/tyssue/config/geometry/__init__.py index e53820c4..54911233 100644 --- a/tyssue/config/geometry/__init__.py +++ b/tyssue/config/geometry/__init__.py @@ -150,3 +150,42 @@ def bulk_spec(): "id": 0, } return spec + +def lateral_spec(): + """ + Specification for a 2D lateral sheet + """ + spec = { + "edge": { + "srce": 0, + "trgt": 0, + "length": 1.0, + "face": 0, + "dx": 0.0, + "dy": 0.0, + "sx": 0.0, + "sy": 0.0, + "tx": 0.0, + "ty": 0.0, + "fx": 0.0, + "fy": 0.0, + "segment": "", + }, + "vert": { + "is_active": 1, + "x": 0.0, + "y": 0.0, + "segment": "", + }, + "face": { + "x": 0.0, + "y": 0.0, + "is_alive": 1, + "perimeter": 0.0, + "area": 0.0, + "num_sides": 6, + "id": 0, + }, + "settings": {"geometry": "lateral"}, + } + return spec diff --git a/tyssue/core/lateralsheet.py b/tyssue/core/lateralsheet.py new file mode 100644 index 00000000..1c828eba --- /dev/null +++ b/tyssue/core/lateralsheet.py @@ -0,0 +1,65 @@ +from .objects import get_prev_edges +from .sheet import Sheet + + +class LateralSheet(Sheet): + """ + 2D lateral model + """ + + def segment_index(self, segment, element): + df = getattr(self, "{}_df".format(element)) + return df[df["segment"] == segment].index + + @property + def lateral_edges(self): + return self.segment_index("lateral", "edge") + + @property + def apical_edges(self): + return self.segment_index("apical", "edge") + + @property + def basal_edges(self): + return self.segment_index("basal", "edge") + + @property + def apical_verts(self): + return self.segment_index("apical", "vert") + + @property + def basal_verts(self): + return self.segment_index("basal", "vert") + + def reset_topo(self): + super().reset_topo() + self.edge_df["prev"] = get_prev_edges(self) + + @staticmethod + def get_line_edges(self, sub_edges): + sub_edges = sub_edges.reset_index() + srces, trgts, edge = sub_edges[["srce", "trgt", "edge"]].to_numpy().T + + # Beginning of the line + start_e = sub_edges.iloc[0]["srce"] + for v in sub_edges["srce"].to_numpy(): + if v not in sub_edges["trgt"].to_numpy(): + start_e = sub_edges[sub_edges['srce'] == v].index[0] + + srce, trgt, edge_ = srces[start_e], trgts[start_e], edge[start_e] + edges = [[srce, trgt, edge_]] + + for i in range(len(srces) - 1): + srce, trgt = trgt, trgts[srces == trgt][0] + edge_ = sub_edges[(sub_edges["srce"] == srce)]["edge"].to_numpy()[0] + edges.append([srce, trgt, edge_]) + return edges + + def get_apical_surface(self): + sub_edges = self.edge_df.loc[self.apical_edges] + return self.get_line_edges(sub_edges) + + def get_basal_surface(self): + sub_edges = self.edge_df.loc[self.basal_edges] + return self.get_line_edges(sub_edges) + diff --git a/tyssue/core/objects.py b/tyssue/core/objects.py index c25a9fd6..7bce26f1 100644 --- a/tyssue/core/objects.py +++ b/tyssue/core/objects.py @@ -9,6 +9,7 @@ import numpy as np import pandas as pd +import networkx as nx from ..geometry.planar_geometry import PlanarGeometry from ..geometry.sheet_geometry import SheetGeometry @@ -151,6 +152,17 @@ def __init__(self, identifier, datasets, specs=None, coords=None, maxbackup=5): self.topo_changed = False self.is_ordered = False + # Add columns of unique_id in order to follow topology change + # Add last unique index value in specs + for elem, df in self.datasets.items(): + self.datasets[elem]['unique_id'] = self.datasets[elem].index + self.specs[elem]['unique_id_max'] = self.datasets[elem].shape[0] + + # Add cell lineage graphe + self.lineage = nx.DiGraph() + self.lineage.add_nodes_from([str(i) for i in self.datasets['face']['unique_id']], + color='grey') + @property def vert_df(self): """The face :class:`pd.DataFrame` containing vertex associated @@ -478,6 +490,57 @@ def sum_cell(self, df): """ return self._lvl_sum(df, "cell") + def _lvl_mean(self, df, lvl): + df_ = df + if isinstance(df, np.ndarray): + df_ = pd.DataFrame(df, index=self.edge_df.index) + elif isinstance(df, pd.Series): + df_ = df.to_frame() + elif lvl not in df.columns: + df_ = df.copy() + df_[lvl] = self.edge_df[lvl] + return df_.groupby(lvl).mean() + + def mean_srce(self, df): + """Means the values of the edge-indexed dataframe `df` grouped by + the values of `self.edge_df["srce"]` + + Returns + ------- + mean : :class:`pd.DataFrame` the mean data, indexed by the source vertices. + """ + return self._lvl_mean(df, "srce") + + def mean_trgt(self, df): + """Means the values of the edge-indexed dataframe `df` grouped by + the values of `self.edge_df["trgt"]` + + Returns + ------- + mean : :class:`pd.DataFrame` the mean data, indexed by the source vertices. + """ + return self._lvl_mean(df, "trgt") + + def mean_face(self, df): + """Means the values of the edge-indexed dataframe `df` grouped by + the values of `self.edge_df["face"]` + + Returns + ------- + mean : :class:`pd.DataFrame` the mean data, indexed by the source vertices. + """ + return self._lvl_mean(df, "face") + + def mean_cell(self, df): + """Means the values of the edge-indexed dataframe `df` grouped by + the values of `self.edge_df["cell"]` + + Returns + ------- + mean : :class:`pd.DataFrame` the mean data, indexed by the source vertices. + """ + return self._lvl_mean(df, "cell") + def get_orbits(self, center, periph): """Returns a dataframe with a `(center, edge)` MultiIndex with `periph` elements. @@ -891,6 +954,12 @@ def get_opposite_faces(self): self.face_df.loc[face_pairs[:, 0], "opposite"] = face_pairs[:, 1] self.face_df.loc[face_pairs[:, 1], "opposite"] = face_pairs[:, 0] + def ordered_edges(self): + """Returns "srce", "trgt", "face" and "edge" indices + organized clockwise for all faces + """ + return self.edge_df.groupby("face").apply(_ordered_edges) + def get_opposite_faces(eptm): warnings.warn("Deprecated, use `eptm.get_opposite_faces()` instead") @@ -898,7 +967,7 @@ def get_opposite_faces(eptm): def _ordered_edges(face_edges): - """Returns "srce", "trgt" and "face" indices + """Returns "srce", "trgt" "face" and "edge" indices organized clockwise for each face. Parameters @@ -909,14 +978,17 @@ def _ordered_edges(face_edges): Returns ------- edges: list of 3 ints - srce, trgt, face indices, ordered + srce, trgt, face, edge indices, ordered """ - srces, trgts, faces = face_edges[["srce", "trgt", "face"]].values.T - srce, trgt, face_ = srces[0], trgts[0], faces[0] - edges = [[srce, trgt, face_]] + face_edges = face_edges.copy() + face_edges["edge"] = face_edges.index + srces, trgts, faces, edge = face_edges[["srce", "trgt", "face", "edge"]].values.T + srce, trgt, face_, edge_ = srces[0], trgts[0], faces[0], edge[0] + edges = [[srce, trgt, face_, edge_]] for face_ in faces[1:]: srce, trgt = trgt, trgts[srces == trgt][0] - edges.append([srce, trgt, face_]) + edge_ = face_edges[(face_edges["srce"] == srce)]["edge"].to_numpy()[0] + edges.append([srce, trgt, face_, edge_]) return edges diff --git a/tyssue/draw/ipv_draw.py b/tyssue/draw/ipv_draw.py index b6456ec8..ad09f71d 100644 --- a/tyssue/draw/ipv_draw.py +++ b/tyssue/draw/ipv_draw.py @@ -129,11 +129,17 @@ def edge_mesh(sheet, coords, **edge_specs): elif hasattr(spec["color"], "__len__"): color = _wire_color_from_sequence(spec, sheet)[:, :3] - u, v, w = coords + if len(coords) == 2: + u, v = coords + z = np.repeat(0, sheet.Nv) + if len(coords) == 3: + u, v, w = coords + z = sheet.vert_df[w], + mesh = ipv.Mesh( x=sheet.vert_df[u], y=sheet.vert_df[v], - z=sheet.vert_df[w], + z=z, lines=sheet.edge_df[["srce", "trgt"]].astype(dtype=np.uint32), color=color, ) diff --git a/tyssue/draw/plt_draw.py b/tyssue/draw/plt_draw.py index c9f5278e..d48c3f0f 100644 --- a/tyssue/draw/plt_draw.py +++ b/tyssue/draw/plt_draw.py @@ -201,15 +201,16 @@ def sheet_view(sheet, coords=COORDS, ax=None, cbar_axis=None, **draw_specs_kw): draw_specs = sheet_spec() spec_updater(draw_specs, draw_specs_kw) - if (ax is None) or (cbar_axis is None): + if (ax is None) and (cbar_axis is None): fig = plt.figure() + grid0 = plt.GridSpec(10, 10) + grid0.update(wspace=0.0) + ax = fig.add_subplot(grid0[:, :9]) else: - fig = ax.get_figure() - - grid0 = plt.GridSpec(10, 10) - grid0.update(wspace=0.0) - - ax = fig.add_subplot(grid0[:, :9]) + if ax is None: + fig, ax = plt.subplots() + else: + fig = ax.get_figure() vert_spec = draw_specs["vert"] if vert_spec["visible"]: diff --git a/tyssue/dynamics/effectors.py b/tyssue/dynamics/effectors.py index 2ab62c16..0f4ac09d 100644 --- a/tyssue/dynamics/effectors.py +++ b/tyssue/dynamics/effectors.py @@ -2,6 +2,7 @@ Generic forces and energies """ import numpy as np +import pandas as pd from ..utils import to_nd from . import units @@ -70,6 +71,40 @@ def get_nrj_norm(specs): # Works on an `Epithelium` object's {cls.element} elements. # """ +class Repulsion(AbstractEffector): + dimensions = units.line_elasticity + magnitude = "cell_repulsion" + label = "Cell Repulsion" + element = "vert" + specs = { + "vert": {"force_repulsion": 1.0, + "v_repulsion": 0.0} + } + + @staticmethod + def energy(eptm): + grid = eptm.vert_df.loc[0, "grid"][0] + x = np.argmin(np.abs([grid[0][:, 0] - x for x in eptm.vert_df["x"]]), axis=1) + y = np.argmin(np.abs([grid[1][0, :] - y for y in eptm.vert_df["y"]]), axis=1) + repulse = [eptm.vert_df.loc[v, "v_repulsion"][0][x[v], y[v]] for v in range(eptm.Nv)] + return np.array(eptm.specs['vert']["force_repulsion"]) * repulse + + @staticmethod + def gradient(eptm): + repulse_u = [] + repulse_v = [] + grid = eptm.vert_df.loc[0, "grid"][0] + + x = np.argmin(np.abs([grid[0][:, 0] - x for x in eptm.vert_df["x"]]), axis=1) + y = np.argmin(np.abs([grid[1][0, :] - y for y in eptm.vert_df["y"]]), axis=1) + for v in range(eptm.Nv): + U, V = np.gradient(eptm.vert_df.loc[v, "v_repulsion"][0], 1, 1) + repulse_u.append(U[x[v], y[v]]) + repulse_v.append(V[x[v], y[v]]) + + grad = np.array(eptm.specs['vert']["force_repulsion"]) * pd.DataFrame(np.array([repulse_u, repulse_v]).T) + grad.columns = ["g" + c for c in eptm.coords] + return grad, None class LengthElasticity(AbstractEffector): diff --git a/tyssue/generation/shapes.py b/tyssue/generation/shapes.py index 339a0c52..1ee904a3 100644 --- a/tyssue/generation/shapes.py +++ b/tyssue/generation/shapes.py @@ -10,6 +10,7 @@ from ..core.monolayer import Monolayer from ..core.objects import Epithelium, get_prev_edges from ..core.sheet import Sheet, get_outer_sheet +from ..core.lateralsheet import LateralSheet from ..geometry.bulk_geometry import ClosedMonolayerGeometry from ..geometry.sheet_geometry import ( ClosedSheetGeometry, @@ -178,6 +179,101 @@ def generate_ring(Nf, R_in, R_out, R_vit=None, apical="in"): return ring +""" +Flat lateral 2D sheet +""" +def generate_lateral_tissue(Nf, length, height): + """ + Generate a 2D lateral tyssue object + + Parameters + ---------- + Nf : int + The number of cells in the tissue + length : int + Length of the tissue + height : float + Height of the cell + + Returns + ------- + eptm : :class:'LateralSheet' + 2D lateral tissue + """ + + specs = config.geometry.lateral_spec() + Ne = Nf * 4 + Nv = Nf * 2 + + vert_df = pd.DataFrame( + index=pd.Index(range(Nv + 2), name="vert"), + columns=specs["vert"].keys(), + dtype=float + ) + + edge_df = pd.DataFrame( + index=pd.Index(range(Ne), name="edge"), + columns=specs["edge"].keys(), + dtype=float + ) + + face_df = pd.DataFrame( + index=pd.Index(range(Nf), name="face"), + columns=specs["face"].keys(), + dtype=float + ) + + inner_edges = np.array( + [ + [f0, v0, v1 + 1] + for f0, v0, v1 in zip(range(Nf), range(Nf), range(Nf)) + ] + ) + + outer_edges = np.zeros_like(inner_edges) + outer_edges[:, 0] = inner_edges[:, 0] + outer_edges[:, 1] = inner_edges[:, 2] + (Nf + 1) + outer_edges[:, 2] = inner_edges[:, 1] + (Nf + 1) + + left_spokes = np.zeros_like(inner_edges) + left_spokes[:, 0] = inner_edges[:, 0] + left_spokes[:, 1] = outer_edges[:, 2] + left_spokes[:, 2] = inner_edges[:, 1] + + right_spokes = np.zeros_like(inner_edges) + right_spokes[:, 0] = inner_edges[:, 0] + right_spokes[:, 1] = inner_edges[:, 2] + right_spokes[:, 2] = outer_edges[:, 1] + + edges = np.concatenate([inner_edges, outer_edges, left_spokes, right_spokes]) + + edge_df[["face", "srce", "trgt"]] = edges + edge_df[["face", "srce", "trgt"]] = edge_df[["face", "srce", "trgt"]].astype(int) + + # Setting vertices position + vert_df.loc[range(Nf + 1), "x"] = [length / Nf * i for i in range(Nf + 1)] + vert_df.loc[range(Nf + 1), "y"] = 0 + vert_df.loc[range(Nf + 1, 2 * (Nf + 1)), "x"] = [length / Nf * i for i in range(Nf + 1)] + vert_df.loc[range(Nf + 1, 2 * (Nf + 1)), "y"] = height + + vert_df["segment"] = "basal" + edge_df["segment"] = "basal" + edge_df.loc[range(Nf, 2 * Nf), "segment"] = "apical" + vert_df.loc[range(Nf + 1, 2 * (Nf + 1)), "segment"] = "apical" + + edge_df.loc[range(2 * Nf, 4 * Nf), "segment"] = "lateral" + + vert_df['is_active'] = 1 + face_df['is_alive'] = 1 + + datasets = {"vert": vert_df, "edge": edge_df, "face": face_df} + + lateral = LateralSheet("lateral", datasets, specs, coords=["x", "y"]) + + lateral.reset_topo() + return lateral + + """ Ellipsoid 2.5D sheet """ diff --git a/tyssue/geometry/planar_geometry.py b/tyssue/geometry/planar_geometry.py index 5ea366c7..e6062366 100644 --- a/tyssue/geometry/planar_geometry.py +++ b/tyssue/geometry/planar_geometry.py @@ -1,6 +1,7 @@ import numpy as np from .base_geometry import BaseGeometry +from skimage.draw import polygon class PlanarGeometry(BaseGeometry): @@ -24,6 +25,7 @@ def update_all(cls, sheet): cls.update_normals(sheet) cls.update_areas(sheet) cls.update_perimeters(sheet) + # cls.update_repulsion(sheet) @staticmethod def update_normals(sheet): @@ -42,6 +44,25 @@ def update_areas(sheet): sheet.edge_df["sub_area"] = sheet.edge_df["nz"] / 2 sheet.face_df["area"] = sheet.sum_face(sheet.edge_df["sub_area"]) + @staticmethod + def update_repulsion(sheet): + # Create globale grid + grid = np.mgrid[np.min(sheet.vert_df['x']) - 0.1:np.max(sheet.vert_df['x']) + 0.1:0.1, + np.min(sheet.vert_df['y']) - 0.1:np.max(sheet.vert_df['y']) + 0.1:0.1] + face_repulsion = gaussian_repulsion(grid, sheet) + + sheet.vert_df['v_repulsion'] = 0 + sheet.vert_df['grid'] = 0 + for v in range(sheet.Nv): + faces = sheet.edge_df[sheet.edge_df["srce"] == v]['face'].to_numpy() + sum_ = np.sum(face_repulsion, axis=2) + sub_ = np.sum(face_repulsion[:, :, faces], axis=2) + v_repulsion = sum_ - sub_ + sheet.vert_df.loc[v, 'v_repulsion'] = [v_repulsion] + sheet.vert_df.loc[v, 'grid'] = [grid] + v_repulsion = None + del v_repulsion + @staticmethod def face_projected_pos(sheet, face, psi): """ @@ -53,10 +74,10 @@ def face_projected_pos(sheet, face, psi): rot_pos = sheet.vert_df[sheet.coords].copy() face_x, face_y = sheet.face_df.loc[face, ["x", "y"]] rot_pos.x = (sheet.vert_df.x - face_x) * np.cos(psi) - ( - sheet.vert_df.y - face_y + sheet.vert_df.y - face_y ) * np.sin(psi) rot_pos.y = (sheet.vert_df.x - face_x) * np.sin(psi) + ( - sheet.vert_df.y - face_y + sheet.vert_df.y - face_y ) * np.cos(psi) return rot_pos @@ -85,8 +106,8 @@ def update_lumen_volume(eptm): apical_edge_pos = (srce_pos + trgt_pos) / 2 apical_edge_coords = eptm.edge_df.loc[eptm.apical_edges, ["dx", "dy"]] eptm.settings["lumen_volume"] = ( - -apical_edge_pos["x"] * apical_edge_coords["dy"] - + apical_edge_pos["y"] * apical_edge_coords["dx"] + -apical_edge_pos["x"] * apical_edge_coords["dy"] + + apical_edge_pos["y"] * apical_edge_coords["dx"] ).values.sum() @@ -134,3 +155,32 @@ def normalize_weights(sheet): .sort_index(level=1) .to_numpy() ) + + +def gaussian_repulsion(grid, sheet): + """ + Parameters + ---------- + + Returns + ------- + X : np.array of x position + Y : np.array of y position + Z : np.array of field repulsion value + """ + shape = list(grid[0].shape) + shape.append(sheet.Nf) + face_repulsion = np.zeros(shape) + face_ord_edges = sheet.ordered_edges() + + for face in range(sheet.Nf): + # apply cell mask + pos_v = list(np.array(face_ord_edges[face]).flatten()[1::4]) + pos_v.append(pos_v[0]) + + xx = np.argmin(np.abs([grid[0][:, 0] - x for x in sheet.vert_df.loc[pos_v, "x"]]), axis=1) + yy = np.argmin(np.abs([grid[1][0, :] - y for y in sheet.vert_df.loc[pos_v, "y"]]), axis=1) + + rr, cc = polygon(xx, yy) + face_repulsion[rr, cc, face] = 1 + return face_repulsion diff --git a/tyssue/io/meshes.py b/tyssue/io/meshes.py index b3c05ef2..827ddd06 100644 --- a/tyssue/io/meshes.py +++ b/tyssue/io/meshes.py @@ -7,6 +7,28 @@ logger = logging.getLogger(name=__name__) +def to_mesh(sheet): + cell_types = { + 3: "triangle", + 4: "quad"} + max_nsides = sheet.face_df["num_sides"].max() + 1 + cell_types.update( + { + i: f'{"polygon"}' for i in range(5, max_nsides) + } + ) + sheet.reset_index(order=True) + sheet.edge_df["f_sides"] = sheet.upcast_face("num_sides") + cells = [ + ('line', sheet.edge_df[["srce", "trgt"]].to_numpy()) + ] + for n, edges in sheet.edge_df.groupby("f_sides"): + polys = np.vstack(edges.groupby("face").apply(lambda e: e["srce"].values).values) + cells.append((cell_types[n], polys)) + mesh = meshio.Mesh(points=sheet.vert_df[sheet.coords].to_numpy(), cells=cells) + return mesh + + def save_triangular_mesh(filename, eptm): coords = eptm.coords eptm.reset_index(order=True) diff --git a/tyssue/topology/base_topology.py b/tyssue/topology/base_topology.py index 4685e18f..920f527d 100644 --- a/tyssue/topology/base_topology.py +++ b/tyssue/topology/base_topology.py @@ -119,11 +119,13 @@ def add_vert(eptm, edge): eptm.edge_df.loc[new_edges, "srce"] = new_vert eptm.edge_df.loc[new_edges, "trgt"] = trgt - eptm.edge_df.loc[opposites.index, "srce"] = new_vert - eptm.edge_df = pd.concat([eptm.edge_df, opposites], ignore_index=True) - new_opp_edges = eptm.edge_df.index[-opposites.index.size :] - eptm.edge_df.loc[new_opp_edges, "trgt"] = new_vert - eptm.edge_df.loc[new_opp_edges, "srce"] = trgt + new_opp_edges = [] + if len(opposites.index): + eptm.edge_df.loc[opposites.index, "srce"] = new_vert + eptm.edge_df = pd.concat([eptm.edge_df, opposites], ignore_index=True) + new_opp_edges = eptm.edge_df.index[-opposites.index.size :] + eptm.edge_df.loc[new_opp_edges, "trgt"] = new_vert + eptm.edge_df.loc[new_opp_edges, "srce"] = trgt # ## Sheet special case if len(new_edges) == 1: @@ -208,6 +210,9 @@ def remove_face(sheet, face): warnings.warn(f"something fishy with face {face}") sheet.edge_df.drop(remanent, axis=0, inplace=True) + sheet.lineage.add_node(str(sheet.face_df.loc[face]['unique_id']), + color='black') + sheet.face_df.drop(face, axis=0, inplace=True) sheet.vert_df.drop(verts, axis=0, inplace=True) diff --git a/tyssue/topology/sheet_topology.py b/tyssue/topology/sheet_topology.py index a32732dd..372533bc 100644 --- a/tyssue/topology/sheet_topology.py +++ b/tyssue/topology/sheet_topology.py @@ -241,6 +241,17 @@ def face_division(sheet, mother, vert_a, vert_b): return daughter +def drop_face(eptm, face, geom, **kwargs): + """ + Removes the face indexed by "face" and all associated edges to allow holes + """ + edge = eptm.edge_df.loc[(eptm.edge_df['face'] == face)].index + + eptm.remove(edge, **kwargs) + eptm.sanitize(trim_borders = True) + geom.update_all(eptm) + + def resolve_t1s(sheet, geom, model, solver, max_iter=60): l_th = sheet.settings["threshold_length"] diff --git a/tyssue/utils/utils.py b/tyssue/utils/utils.py index f08aee62..4d2417d0 100644 --- a/tyssue/utils/utils.py +++ b/tyssue/utils/utils.py @@ -103,7 +103,7 @@ def set_data_columns(datasets, specs, reset=False): df[col] = default -def data_at_opposite(sheet, edge_data, free_value=None): +def data_at_opposite(sheet, edge_data, free_value=None, column=""): """ Returns a pd.DataFrame with the values of the input edge_data at the opposite edges. For free edges, optionaly replaces Nan values @@ -113,6 +113,8 @@ def data_at_opposite(sheet, edge_data, free_value=None): ---------- sheet: a :class:`Sheet` instance edge_data: dataframe contain value of edge + free_value: value used to replace Nan + column: string: column name so store opposite value in Sheet.edge_df Returns ------- @@ -136,8 +138,10 @@ def data_at_opposite(sheet, edge_data, free_value=None): ) if free_value is not None: opposite = opposite.replace(np.nan, free_value) - - return opposite + if column: + sheet.edge_df[column] = opposite + else: + return opposite def get_sub_eptm(eptm, edges, copy=False):