diff --git a/covidRefSequences/PacBioHiFi.mmi b/covidRefSequences/PacBioHiFi.mmi new file mode 100755 index 0000000..054cfb2 Binary files /dev/null and b/covidRefSequences/PacBioHiFi.mmi differ diff --git a/nextflow.config b/nextflow.config index f023e1e..27480a3 100755 --- a/nextflow.config +++ b/nextflow.config @@ -60,9 +60,10 @@ process { } withName: 'freyjaVariantCaller' { - time = '2 h' + time = '3 h' maxForks = 20 cpus = 15 + // cpus = 20 memory = '24 GB' } diff --git a/startWorkflow.nf b/startWorkflow.nf index ad0fb5a..b0ce8b1 100755 --- a/startWorkflow.nf +++ b/startWorkflow.nf @@ -152,7 +152,7 @@ process referenceAlignment { minimap2 -a --sam-hit-only -2 -x map-ont ${refSeqBasename}.mmi R1.fastq.gz -t \$numThreads -o aligned.sam ;; PacBio) - minimap2 -a --sam-hit-only -2 -x map-hifi ${refSeqBasename}.mmi R1.fastq.gz -t \$numThreads -o aligned.sam + minimap2 -a --sam-hit-only -2 -x map-hifi $projectDir/covidRefSequences/PacBioHiFi.mmi R1.fastq.gz -t \$numThreads -o aligned.sam ;; esac """