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The pipeline fails on bam2bg (and others downstream) when running with "no_dedup" option due to mismatch with column names.
Solution: Replace "nodedup" with "no_dedup"
workflow/scripts/_get_nreads_stats.py, line 29
-stats["nodedup_nreads_genome"] = sum(list(nodedup_idxstats[nodedup_idxstats[0].isin(list(genomelen[0]))][2])) +stats["no_dedup_nreads_genome"] = sum(list(nodedup_idxstats[nodedup_idxstats[0].isin(list(genomelen[0]))][2])) -stats["nodedup_nreads_spikein"] = sum(list(nodedup_idxstats[nodedup_idxstats[0].isin(list(spikeinlen[0]))][2])) +stats["no_dedup_nreads_spikein"] = sum(list(nodedup_idxstats[nodedup_idxstats[0].isin(list(spikeinlen[0]))][2]))
workflow/scripts/_make_alignment_stats_table.R, line 54
- "nodedup_nreads_genome", + "no_dedup_nreads_genome", - "nodedup_nreads_spikein", + "no_dedup_nreads_spikein",
workflow/scripts/_make_library_norm_table.R, line 49
-for (dedup_type in c("dedup_nreads_genome","nodedup_nreads_genome")){ +for (dedup_type in c("dedup_nreads_genome","no_dedup_nreads_genome")){
workflow/scripts/_make_library_norm_table.R, line 73 + df$dedup_type=gsub("no","no_dedup",df$dedup_type)
+ df$dedup_type=gsub("no","no_dedup",df$dedup_type)
The text was updated successfully, but these errors were encountered:
Recapitulated with test data, but only when using "library" normalization instead of "spikein"
Sorry, something went wrong.
slsevilla
Successfully merging a pull request may close this issue.
The pipeline fails on bam2bg (and others downstream) when running with "no_dedup" option due to mismatch with column names.
Solution: Replace "nodedup" with "no_dedup"
workflow/scripts/_get_nreads_stats.py, line 29
workflow/scripts/_make_alignment_stats_table.R, line 54
workflow/scripts/_make_library_norm_table.R, line 49
workflow/scripts/_make_library_norm_table.R, line 73
+ df$dedup_type=gsub("no","no_dedup",df$dedup_type)
The text was updated successfully, but these errors were encountered: