Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

run BUS on inDrop samples nomenclature fastq files #6

Open
droplet-lab opened this issue Jun 21, 2019 · 0 comments
Open

run BUS on inDrop samples nomenclature fastq files #6

droplet-lab opened this issue Jun 21, 2019 · 0 comments

Comments

@droplet-lab
Copy link

Thank you for this amazing tool.

I was wondering how to run inDrop files on BUS. From the notebooks, I can see that the initial step is ran using:
kallisto bus -i index/Mus_musculus.GRCm38.cdna.all.fa.kidx -x inDrop -t 8 -o kallisto_activity_out/B1_1_0h \ indrop/B1/SRR5945694_R2.fastq.gz indrop/B1/SRR5945694_R1.fastq.gz

I was wondering if you could elaborate on what R1 and R2 stand for. I assume R1 is the biological read and R2 is dualbarcode+UMI. I was wondering if the tools could also take a library tag into account for demultiplexing (or should I manually demultiplex and run samples independently?

Would you please be able to confirm this is the expected nomenclature?

Thanks !!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant