diff --git a/tools/spot_detection_2d/spot_detection_2d.py b/tools/spot_detection_2d/spot_detection_2d.py
index 80f5a0f0..473fba3a 100644
--- a/tools/spot_detection_2d/spot_detection_2d.py
+++ b/tools/spot_detection_2d/spot_detection_2d.py
@@ -87,6 +87,12 @@ def spot_detection(
         blobs = blob_filter(img, threshold=abs_threshold, threshold_rel=rel_threshold, min_sigma=min_scale, max_sigma=max_scale)
         for blob in blobs:
             y, x, scale = blob
+
+            # Skip the detection if it is too close to the boundary of the image
+            if y < boundary or x < boundary or y >= img.shape[0] - boundary or x >= img.shape[1] - boundary:
+                continue
+
+            # Add the detection to the list of detections
             radius = scale * np.sqrt(2) * 2
             intensity = mean_intensity(img, round(y), round(x), round(radius))
             detections.append(
diff --git a/tools/spot_detection_2d/test-data/output1.tsv b/tools/spot_detection_2d/test-data/output1.tsv
index a2eb0825..15d59c59 100644
--- a/tools/spot_detection_2d/test-data/output1.tsv
+++ b/tools/spot_detection_2d/test-data/output1.tsv
@@ -406,7 +406,6 @@ frame	pos_x	pos_y	scale	radius	intensity
 3	214	178	1.00	2.83	9065.83
 3	42	86	1.00	2.83	11837.97
 3	265	177	1.56	4.40	20759.84
-3	256	188	1.00	2.83	19457.31
 3	67	154	1.00	2.83	39978.07
 3	318	154	1.33	3.77	9323.84
 3	170	176	1.11	3.14	13875.28
@@ -740,7 +739,6 @@ frame	pos_x	pos_y	scale	radius	intensity
 5	214	178	1.00	2.83	9065.83
 5	42	86	1.00	2.83	11837.97
 5	265	177	1.56	4.40	20759.84
-5	256	188	1.00	2.83	19457.31
 5	67	154	1.00	2.83	39978.07
 5	318	154	1.33	3.77	9323.84
 5	170	176	1.11	3.14	13875.28
diff --git a/tools/spot_detection_2d/test-data/output2.tsv b/tools/spot_detection_2d/test-data/output2.tsv
index ba0c7e74..47efffa6 100644
--- a/tools/spot_detection_2d/test-data/output2.tsv
+++ b/tools/spot_detection_2d/test-data/output2.tsv
@@ -7,11 +7,9 @@ frame	pos_x	pos_y	scale	radius	intensity
 1	61	27	1.56	4.40	18052.18
 1	158	39	1.44	4.09	18377.02
 1	190	14	1.33	3.77	18548.86
-1	24	75	1.56	4.40	23031.16
 1	182	33	1.78	5.03	26467.79
 1	39	39	1.44	4.09	14782.43
 1	169	26	1.33	3.77	14203.41
-1	10	83	2.00	5.66	21493.92
 1	61	54	1.33	3.77	23248.06
 1	95	52	1.33	3.77	21480.71
 1	23	60	1.89	5.34	25203.43
@@ -20,16 +18,12 @@ frame	pos_x	pos_y	scale	radius	intensity
 1	66	49	1.11	3.14	23858.07
 1	115	36	2.00	5.66	16389.10
 1	55	51	1.33	3.77	23548.90
-1	201	21	2.00	5.66	25498.97
 1	130	72	1.67	4.71	15769.02
-1	151	78	1.78	5.03	18372.90
 1	117	23	1.33	3.77	16763.14
 1	45	52	1.56	4.40	22877.61
 1	36	71	1.56	4.40	20780.96
-1	0	50	1.56	4.40	19538.34
 1	78	17	1.33	3.77	16844.51
 1	101	38	1.56	4.40	21376.59
-1	183	6	1.78	5.03	16774.07
 1	147	31	1.78	5.03	16597.14
 1	163	55	2.00	5.66	18301.54
 1	164	23	1.33	3.77	17073.82
@@ -40,9 +34,6 @@ frame	pos_x	pos_y	scale	radius	intensity
 1	69	17	1.11	3.14	15601.83
 1	83	52	1.33	3.77	18315.00
 1	16	54	2.00	5.66	22140.66
-1	12	0	2.00	5.66	17703.35
-1	175	2	1.11	3.14	14856.11
 1	166	61	1.78	5.03	18488.78
 1	163	43	1.44	4.09	16925.49
 1	130	53	1.78	5.03	15101.96
-1	204	32	2.00	5.66	25289.27