diff --git a/changelog/update-e2e-setup-script-env-var b/changelog/update-e2e-setup-script-env-var
new file mode 100644
index 00000000000..457978d1fae
--- /dev/null
+++ b/changelog/update-e2e-setup-script-env-var
@@ -0,0 +1,5 @@
+Significance: patch
+Type: dev
+Comment: Define WooPay Blog ID for e2e env setup
+
+
diff --git a/tests/e2e/README.md b/tests/e2e/README.md
index 7d77591fd19..35b5e5c5e5f 100644
--- a/tests/e2e/README.md
+++ b/tests/e2e/README.md
@@ -45,6 +45,7 @@ E2E_WCPAY_STRIPE_TEST_SECRET_KEY=<stripe sk_test_xxx>
 E2E_WCPAY_STRIPE_TEST_WEBHOOK_SIGNATURE_KEY=<stripe whsec_xxx>
 # This should be the Stripe Account ID of a connected merchant account. For example, after onboarding an account, you can obtain the ID from WCPay Dev Tools.
 E2E_WCPAY_STRIPE_ACCOUNT_ID=<stripe acct_id>
+E2E_WOOPAY_BLOG_ID=<WPCOM Site ID for https://pay.woo.com>
 ```
 
 **Using Live Server**
diff --git a/tests/e2e/env/setup.sh b/tests/e2e/env/setup.sh
index 04ddafd834e..50d4313f235 100755
--- a/tests/e2e/env/setup.sh
+++ b/tests/e2e/env/setup.sh
@@ -61,6 +61,7 @@ if [[ "$E2E_USE_LOCAL_SERVER" != false ]]; then
 	define( 'WCPAY_STRIPE_LIVE_SECRET_KEY', 'sk_live_XXXXXXX' );
 	define( 'WCPAY_ONBOARDING_ENCRYPT_KEY', str_repeat( 'a', SODIUM_CRYPTO_SECRETBOX_KEYBYTES ) );
 	define( 'WOOPAY_URL', 'https://pay.woo.com' );
+	define( 'WOOPAY_BLOG_ID', '$E2E_WOOPAY_BLOG_ID' );
 	"
 	printf "$SECRETS" > "local/secrets.php"
 	echo "Secrets created"