Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Question about -f and -v input file? #5

Open
YAYA530 opened this issue Mar 16, 2022 · 1 comment
Open

Question about -f and -v input file? #5

YAYA530 opened this issue Mar 16, 2022 · 1 comment

Comments

@YAYA530
Copy link

YAYA530 commented Mar 16, 2022

I'm confused a little bit about the input fils -f: input genomes/scaffolds and -v: prophage coordinates input from VIBRANT. I want to analyze the activation prophage in several case/control samples but I'm not sure if input scaffolds and prophage coordinates files are individual sample or these two files should combine all samples' scaffold together (like a database)?
So far, I finished reads assembly into scaffold in each sample. May I know whether I should combine all samples' scaffold together and do VIBRANT then do PropagAtE OR I should do VIBRANT and PropagAtE in each samples' assembly files?

@KrisKieft
Copy link
Member

You can do either option mentioned. It depends on what you are interested in identifying/studying and what you hope to accomplish from your data. Based on the information given, I'd suggest running VIBRANT and PropagAtE separately on all samples (option 2).

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants