You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hello! I'm processing samples with multiple replicates, and I've found that the system works fine as long as the number of treated and untreated samples is the same. But in many cases, I have a single mock file and 2-8 treated samples.
I've tried setting my command script up with tsamp = "T1name, T2name, T3name..." and usamp = "U1name", but each time the pipeline gets stuck during overlap analysis:
"################ DELTA AND HITS ################ "
"CAST0CCR5OT0WTRNP0DUP_S1_L001 vs. CAST0CCR5OT0MOCK_S1"
"Error: File not found -> ../samples/CCR5_OT_WTRNP/U2/fastq_aln/CAST0CCR5OT0MOCK_S1_Alignment.bed"
"################ OVERLAP ANALYSIS ################"
"../samples/CCR5_OT_WTRNP/OVL2_SIGNIF1"
Error in read.xlsx.default(X[[i]], ...) : File does not exist.
Calls: runPipelineCRISPR ... getUnion -> lapply -> lapply -> FUN -> read.xlsx.default
Execution halted
I can get around this by simply duplicating the U1 file and changing the name slightly, but it massively increases the processing time because it treats each untreated file as a distinct sample. Is there a simpler way to do this?? Thank you!
The text was updated successfully, but these errors were encountered:
Hello! I'm processing samples with multiple replicates, and I've found that the system works fine as long as the number of treated and untreated samples is the same. But in many cases, I have a single mock file and 2-8 treated samples.
I've tried setting my command script up with tsamp = "T1name, T2name, T3name..." and usamp = "U1name", but each time the pipeline gets stuck during overlap analysis:
"################ DELTA AND HITS ################ "
"CAST0CCR5OT0WTRNP0DUP_S1_L001 vs. CAST0CCR5OT0MOCK_S1"
"Error: File not found -> ../samples/CCR5_OT_WTRNP/U2/fastq_aln/CAST0CCR5OT0MOCK_S1_Alignment.bed"
"################ OVERLAP ANALYSIS ################"
"../samples/CCR5_OT_WTRNP/OVL2_SIGNIF1"
Error in read.xlsx.default(X[[i]], ...) : File does not exist.
Calls: runPipelineCRISPR ... getUnion -> lapply -> lapply -> FUN -> read.xlsx.default
Execution halted
I can get around this by simply duplicating the U1 file and changing the name slightly, but it massively increases the processing time because it treats each untreated file as a distinct sample. Is there a simpler way to do this?? Thank you!
The text was updated successfully, but these errors were encountered: